最新刊期
卷 19 ， 期 1 ， 2013
- 摘要：Objective:To prepare uniform-sized silybin loaded poly (lactic-co-glycolic acid) (PLGA) microspheres and optimize its preparation technology. Method: Silybin PLGA microspheres was prepared by premix membrane emulsification-solvent extraction/evaporation method,with the average particle size and span as indexes,oil phase solvent,membrane pore size,membrane pressure and volume ratio of oil-water phase were investigated by single factor test,influence of pharmaceutical-accessories ratio,the mass fraction of PVA and volume ratio of oil-water phase on preparation technology was investigated by orthogonal test;The mean particle size,particle size distribution,drug loading,entrapment efficiency,morphology and other physical-chemical properties were evaluated. Result: Pore size of SPG membrane was 2.8 μm,membrane pressure was 1.0 MPa,centrifuged time of 20 min,physiological saline as hardening liquid;Optimum preparation technology of silybin PLGA microspheres was as following:pharmaceutical-accessories ratio of 1:4,the mass fraction of PVA of 3%,volume ratio of oil-water phase of 1:19.Prepared microspheres were round with smooth surface,the mean diameter was (2.634±0.35) μm,span was (13.326±3.06),drug loading was (14.84±0.76)% and entrapment efficiency was (56.16±3.77)%. Conclusion: Premix membrane emulsification method could be used to prepare silybin PLGA microspheres,which was an insoluble component from Chinese meteria medica,and particle size of prepared silybin PLGA microspheres was uniform and controllable.关键词：premix membrane emulsification method;silybin;PLGA microspheres;uniformity
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of Cornus officinalis,and investigate its purification technology by macroporous resin. Method: With the content of morroniside as index,the concentration of ethanol,solid-liquid ratio,extraction time and times were selected as factors,extraction technology of total glycosides was optimized by single factor test and orthogonal test.With sampling volume,elution speed,eluent concentration and dosage as factors,single factor test was used to investigate purification technology of C. officinialis with HPD-300 macroporous resin. Result: Optimum extraction technology was as following:extracted 3 times with 50% ethanol of 8 times amount,3 h each time.Purification technology was:eluted with 3 BV 30% ethanol at 1.5 BV·h-1,elution rate of 94.9%. Conclusion: Optimized extracting technology was stable and feasible;It showed that total glycosides from C. officinalis could be effectively purify by HPD-300 macroporous resin.关键词：Cornus officinalis;total glycosides;orthogonal test;extraction process;Macroporous resin
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发布时间：2024-01-04 - 摘要：Objective:To investigate spray drying process of Coptidis Rhizoma extract. Method: With the amount of spray dry powder,the content of berberine and moisture content as indexes,effect of air inlet temperature,outlet temperature and extract specific gravity on spray drying process of Coptidis Rhizoma extract was investigated by orthogonal test. Result: Optimal spray drying process was:air inlet temperature 180℃,outlet temperature 80℃ and extract specific gravity of 1.15. Conclusion: This optimized process was rational and stable,it could provide experimental basis for industrial production of Coptidis Rhizoma.关键词：orthogonal test;spray drying;Coptidis Rhizoma extract;powder amount;moisture content;berberine
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发布时间：2024-01-04 - 摘要：Objective:Taking Gegen Qinlian decoction as study object,To study comprehensively on relationship between parameters which influence quality of decoction (such as water amount,decocting time,et al.) and dissolution of main ingredients,and obtain optimum decocting process of Gegen Qinlian decoction. Method: Decocting time had 20,35,50,65,80 min;the amount of water were 6,9,12,15,18 times;decocting times of one time;Decocting process was determined by permutation and combination of the above parameters with comprehensive experimental method.Each process of modified Gegen Qinlian decoction took one formula to decoct three times.The content of main ingredients were determined by HPLC,and influence of different decocting processes on dissolution was compared. Result: ①Under the same decocting time and decocting times,dissolution of main ingredients showed a upward trend with increasing of water amount,significant changeed at 9 times the amount of water.②Under the same water amount and decocting times,while different decocting time,dissolution of main ingredients showed a upward trend with extension of decocting time,obvious change point at 50 min. Conclusion: The minimum amount of water for Gegen Qinlian decoction was 9 times,decocting time was 50 min.关键词：Gegen Qinlian decoction;decocting times;water amount;decocting time;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction conditions of total saponins from Panax japonicus by foam separation technique. Method: Taking enrichment ratio,purity ratio and recovery as indexes,effect of pH,bubble size,temperature of mixture,electrolyte concentration and other main influencing factor on foam separation was investigated,single factor test was used to optimize extraction technology of total saponins from P. japonicus by foam separation. Result: Optimum extraction conditions were as followings:bubble diameter 0.4-0.5 mm,temperature 65℃, pH 5.6,the concentration of NaCl 0.015 mol·L-1,under these conditions,enrichment ratio of total saponins was 2.1,purity ratio was above 2.6 and recovery was 98.33%. Conclusion: Foam separation technique could be applied to separate total saponins from P. japonicus with stability,reliability and high yield,it provided a reference for development and utilization of natural total saponins in P. japonicus.关键词：foam separation;saponins from Panax japonicus;enrichment ratio;recovery;purity ratio
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发布时间：2024-01-04 - 摘要：Objective:To establish a simple,feasible and precise quality control strategy for determination of concentration and entrapment efficiency of isopropylidene shikimic acid liposome. Method: Chromatographic conditions were: Agilent Eclipse Plus C18 column (4.6 mm×100 mm,3.5 μm),column temperature of 30℃,mobile phase of acetonitrile-0.05% phosphoric acid solution(10:90),flow rate of 1.0 mL·min-1,injection volume of 20 μL and detection wavelength at 220 nm.The content of ISA in liposomes was determined by HPLC;Encapsulation efficiency was determined by ultrafiltration,gel chromatography and dialysis method,respectively,and results were compared with each other. Result: After demulsification by adding 4 times the amount of methanol into ISA liposome and high speed centrifugal sedimentation,supernatant was used to determine,determination results showed a good specificity, precision and accuracy,regression equation showed a good linearity (r=0.999 9) within range of 1.004-150.6 mg·L-1,recovery was (102.01±1.18)%,reference substance solution of ISA was stable in 6 h;Entrapment efficiency of ISA liposome determined by ultrafiltration method,gel permeation method and dialysis method were (92.96±1.91)%, (91.23±2.23)%,(73.66±7.10)%,respectively. Conclusion: This established HPLC method was stable and reliable,it could be used for quality control and in vitro analysis;In determination of encapsulation efficiency,ultrafiltration method was simple and efficient,its results were close to gel permeation chromatography;Due to limit of measurement conditions,determination result of dialysis method might be lower for its time-consuming process and higher temperature, and also depending on stability of liposome solution and existence state of drugs in liposome.关键词：isopropylidene shikimic acid;entrapment efficiency;ultrafiltration method;dialysis method;gel column chromatography;precipitation with methanol
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发布时间：2024-01-04 - 摘要：Objective:To prepare single-chamber and single-layer osmotic pump-controlled release tablets of bergenin with high drug loading,which was a poorly water-soluble drug. Method: Solid dispersion was prepared with common excipients of osmotic pump tablets as mixed carrier material,prescription of tablets core was optimized by isoosmotic principle;With cumulative release of bergenin as index,coating formulation was selected by central composite design-response surface methodology. Result: When the mass fraction of PEG 1500 was 1.30% and coating gain was 9.5%,cumulative release of prepared osmotic pump tablets of bergenin with high drug loading was nearly 80%,it fitted zero-order release profile. Conclusion: It could effectively increase drug loading of osmotic pump tablets by giving full play to functions of excipients;Methods of optimizing formulation of tablet core and coating could significantly shorten experiment cycle,and this study could provide a new approaches of prescription design of osmotic pump preparation.关键词：poorly water-soluble drug;bergenin;mixed carrier;isoosmotic principle;central composite design
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发布时间：2024-01-04 - 摘要：Objective:To investigate dynamic changes of chemical constituents before and after concentration process of Chuanxiong Chatiao granules by membrane technique. Method: Single-factor test was used to optimize membrane technique industrial production process parameters of Chuanxiong Chatiao granules,by combining with quality standard and HPLC characteristic spectrum of Chuanxiong Chatiao granules,chemical constituents changes of Chuanxiong Chatiao granules with membrane technique and traditional concentration technique were compared. Result: Optimal membrane process parameters were:adopted SMN-130A 2350054 membrane with concentration pressure of 17×104 Pa,concentration temperature of 36℃;Comparative studies of HPLC Characteristic spectrum and quality standards showed that the characteristic peaks’ quantity was equal and peak area was no significant differences of samples,which were prepared by 2 kinds of process,their similarity was 0.97. Conclusion: Membrane technique could be used in industrial production of Chuanxiong Chatiao granules.关键词：Chuanxiong Chatiao granules;membrane technique;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective:To optimize processing technology for Evodia rutaecarpa with Zingiber officinale. Method: Fingerprints of hydrophilic and lipophilic components in E. rutaecarpa were selected as indexes,single factor test was employed to investigate impact of four factors on processing technology of E. rutaecarpa,including the amount of Z. officinale,stuffy embellish time,processing temperature and time. Result: The amount of Z. officinale, processing temperature and time had significant impact on fingerprint of E. rutaecarpa,optimum processing technology was as following:ratio of Z. officinale and raw material 7:100,stir-baked 8 min at 150℃ after moistened 2 h. Conclusion: Optimized processing technology was stable and feasible,it could provide experimental basis for standardization for processing technology of E. rutaecarpa with Z. officinale.关键词：fingerprint;processing Evodia rutaecarpa with Zingiber officinale;processing technology
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of total flavonoids in pinecone of Pinus yunnanensis. Method: Total flavonoids in pinecone of P. yunnanensis were extracted by ultrasonic assisted extraction method,with extraction rate of total flavonoids as index,orthogonal test was applied to investigate influence of four factors (including ratio of material-liquid,ultrasonic extraction temperature,ethanol concentration and extraction time) on extraction technology of total flavonoids from pinecone of P. yunnanensis. Result: Optimum extraction technology was as following:ultrasonic assisted extracted 30 min with 30 times the amount of 60% ethanol at 60℃,the content of total flavonoids in pinecone of P. yunnanensis was 3.23%. Conclusion: Optimized extraction technology was simple and stable with high extraction rate of total flavonoids.关键词：Pinus yunnanensis;pinecone;total flavonoids;orthogonal test;extraction technology
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of Baiqing gel. Method: With the content of paeonol as index,distillation extraction technology of Cynanchum paniculatum was investigated by single factor test;With the content of matrine,berberine hydrochloride and extract yield as comprehensive index,soaking time,the amount of water reflux times and time were chosen as factors,extraction technology of Baiqing gel was optimized by orthogonal test. Result: Distillate was collected with 10 times the amount of C. paniculatum,dregs(after distillation),Phellodendron chinense, Sophora flavescens and other herbal pieces were extracted 3 times with 10 times the amount of water,1.5 h each time. Conclusion: This optimized technology was rational and stable with high extraction rate.
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of total flavonoids from Lysimachia paridiformis,and investigate its in vitro antopxidation. Method: With yield of total flavonoids as index,single factor and orthogonal test were used to optimized extraction technology of total flavonoids from L. paridiformis with solid-liquid ratio,the concentration of ethanol,reflux time and temperature as factors;Scavenging action and reducing power of total flavonoids on hydroxyl radical and superoxide anion free radical were determined by UV spectrophotometry. Result: Optimum extraction technology of total flavonoids from L. paridiformis was:ethanol concentration 65%,ratio of solid-liquid 1:25,reflux temperature 65℃,extraction time 4 h,yield of total flavonoids of 1.69%;Total flavonoids from L. paridiformis had good reducing power and ability to inhibit free radical,and reducing power and scavenging hydroxyl radical activity increased with increasing the concentration of total flavonoids. Conclusion: total flavonoids from L. paridiformis had significant antopxidation.关键词：Lysimachia paridiformis;flavonoids;extraction technology;antopxidation
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发布时间：2024-01-04 - 摘要：Objective:To grope preparation technology of β-cyclodextrin(β-CD) inclusion compound of volatile oil in Guanxinkang capsule,in order to improve stability of volatile oil. Method: Inclusion complexes was prepared by saturated aqueous solution ultrasonic method,with inclusion rate of volatile oil,inclusion oil content and inclusion yield as comprehensive evaluation index,influence of ratio of β-CD and volatile oil,inclusion temperature and ultrasonic time on inclusion technology was investigated by central composite design,test data was fitted by multi-linear regression and binomial equation,inclusion technology conditions were optimized by response surface method,and to predictive analysis. Result: Optimum inclusion process conditions were as follows:ratio of β-CD and volatile oil of 7: 1,inclusion temperature at 45℃ and ultrasonic time of 40 min,deviation between observed and predicted values was less than 3%. Conclusion: Volatile oil was powdered and its stability had been increased after being included by β-CD.This optimized inclusion process was simple with high predictability,it could provide test basis for industrial production of Guanxinkang capsule.关键词：Guanxinkang capsule;volatile oil;central composite design-response surface methodology;β-cyclodextrin;inclusion technique
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发布时间：2024-01-04 - 摘要：Objective:To explore indexes in quality control of decocted extract. Method: Under guidance of traditional concentrated and cream collection experience,to determine relative density,moisture content,kinetic viscosity and other parameters of semi-finished extract and decocted extract,and correlation study of the above indexes was carried out. Result: Three indexes(relative density,moisture content,kinetic viscosity) was stable,reliable and easily measured,relative density had a negative correlation with moisture content. Conclusion: Relative density, moisture content and kinetic viscosity could be selected as reasonable indexes in quality control of decocted extract,this study provided experimental basis for establishment of decocted extract quality standard.关键词：decocted extract;quality control;relative density;moisture content;kinetic viscosity
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发布时间：2024-01-04 - 摘要：Objective:To optimize preparation technology of Changyan granule,and investigate its precipitation and molding technology. Method: Taking composite score of dry extract rate and berberine hydrochloride content as an indicator,orthogonal test was used to optimize extraction technology of Changyan granule with extraction time,extraction times and the amount of water as factors;Its precipitation technology was investigated by single factor test;With granule formability,mobility,hygroscopicity and pelletization as indexes,kinds of accessories were selected,and to determine the best moulding technology. Result: Optimum extraction technology was as following: decoction extracted 2 times with 10 times the amount of water,1.5 h per time;The concentration of alcohol precipitation of 60%;Optimal granulation technology was as following:the ratio of extract-icing sugar-dextrin 1: 2: 1, the critical relative humidity of61%. Conclusion: Optimized technologies of Changyan granule was stable and feasible,it could be applied to industrial production.关键词：Changyan granule;orthogonal test;berberine hydrochloride;preparation technology
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发布时间：2024-01-04 - 摘要：Objective:To optimize water extraction technology of total flavones from Crataegus pinnatifida. Method: Taking yield of total flavonoids as index,the content of total flavonoids was determined by UV,orthogonal test method was used to optimize ultrasonic extraction technology of total flavonoids with extraction time,ratio of solid-liquid,extraction temperature and powder size as factors. Result: Influence sequence of 4 factors on soaking technology was:extraction temperature>powder size>extraction time>ratio of solid-liquid,optimum extraction technology was as following:extraction temperature 95℃,ratio of solid-liquid 1:300,extraction time 10 min, gringing degree 60 mesh,average yield of total flavonoids could be up to 9.36 g·L-1. Conclusion: Optimized water extraction condition was stable and feasible,it could be used for taking of C. pinnatifida teabag.关键词：orthogonal test;soaking with water;total flavones from Crataegus pinnatifida
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发布时间：2024-01-04 - 摘要：Objective:To optimize clarification process for extract of Aike capsule by chitosan. Method: With the content of paeoniflorin,saikosaponin-a and extract yield as comprehensive evaluation index,solid-liquid ratio,the amount of chitosan,stirring temperature and stirring time were selected as factors,orthogonal test was carried out to optimize clarification process of Aike capsule. Result: Optimum clarification process of Aike capsule extract was:solid-liquid ratio 1: 5,stirring time 10 min at 60℃,added chitosan 14 mL per 100 mL extraction solution. Conclusion: This optimized process had advantages of good stability,low cost and high extraction rate of paeoniflorin and saikosaponin-a,it was suitable for clinical development of Aike capsule.关键词：chitosan;paeoniflorin;saikosapoin-a;refining process
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发布时间：2024-01-04 - 摘要：Objective:To optimize flash extraction technology of fresh Rehmannia glutinosa. Method: With catalpol and yield of dry extract as indexes,extraction time,extraction times and solvent consumption were chosen as factors,flash extraction technology of fresh R. glutinosa was optimized by orthogonal test and single factor test.The content of indicator ingredients was determined by HPLC. Result: Optimum flash extraction process was:extrated 1 times with 3 times the amount of 95% ethanol,extraction time of 3 minutes. Conclusion: This optimized process was fast,scientific and reasonable.关键词：fresh Rehmannia glutinosa;flash extraction;orthogonal test;catalpol;yield of dry extract
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发布时间：2024-01-04 - 摘要：Objective:To prepare chemical marker and establish HPLC method for determining this marker in the whole herb of Flickingeria fimbriata. Method: A Pak-C18(4.6 mm×250 mm,5 μm)column was adopted with a mobile phase of acetonitrile-water (20:80) at the flow rate of 1.0 mL·min-1 and the column temperature was set at 25℃. The detection wavelength was 340 nm. Result: Vitexin 2″-O-β-D-glucopyranoside was firstly isolated as a chemical marker from F. fimbriata. This marker showed a good linear relationship in the range of 0.016-1.6 μg(r=0.999 8)and the average recovery was 99.66% (RSD 1.05%). Conclusion: The method is quick, simple, reliable, and reproducible, which can be used for quality control of F. fimbriata.关键词：Flickingeria fimbriata;Orchidaceae;Flickingeria;vitexin 2″-O-β-D-glucopyranoside;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To explore the effects of processing on the content and components on the fatty acid in Semen Raphani and decocting. Method: The content of the fatty acid in the product and decocting was determined by gravimetric method. The oil from Semen Raphani was esterified with carbinol and analyzed by GC-MS. Result: The major fatty acids in Semen Raphani were 13-(Z)-docosenoic acid, 9-(Z)-octadecenoic acid, 9,12-(Z,Z)-octad-ecadienoic acid,9,12,15-(Z,Z,Z)-octadecatrienoic acid, 11-eicosenoic acid, hexadecanoic acid, octadecanoic acid, eicosanoic acid and behenic acid. Conclusion: The constituents and reletive content on the fatty acid in crude Semen Raphani were very close to the fatty acid in roasted Semen Raphani. The processing of Semen Raphani facilitate the quantity of fatty acid, and had no effect on the constituents and reletive content on the fatty acid.关键词：Semen Raphani;the crude and roasted products;fatty acids;GC-MS
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发布时间：2024-01-04 - 摘要：Objective:To analyze the chemical components of the essential oil from different parts of the Ilex hainanensis and provide scientific basis for its quality control. Method: The volatile oil from the leaves,stem of I. hainanensis was obtained by steam distillation and analyzed by gas chromatography-mass spectrometry. The relative content was determined by area normalization. Result: Twenty-four components were identified from the stem, in which accounting for 94.97% of total volatile oil. 33 components were identified from the leaves, in which accounting for 98.48% of total volatile oil. Stems and leaves contain volatile oil composition of compounds:furfural, (2E,4E)-hexadiene, (6Z)-2,6-dimethyl-2,6-octadiene,hexylacetate,dipentene,1-octanol,5-ethenyltetrahydro-.alpha,alpha-5-trimethyl-,cis-2-furanmethanol,terpinolen,inalool,alpha-terpineol,beta-cyclocitral,(Z)-geraniol,geraniol, cyclohexyl n-hexanoate,irisone. Conclusion: The results can be used as the quality standard and provide scientific basis for further development and utilization of I. hainanensis.关键词：ilex hainanensis;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for determination of nine elements of Ca, Mg, Fe, Mn, Cu, Zn, Al, Sr, Sn and Pb in the kernels of Prunus mongolica. Method: The samples were digested by nitric acid and perchloric acid. The content of nine elements in the kernels of P. mongolica was determined by ICP-AES, a blank experiment was made at the same time. Result: The results showed that the linear relationship was good and the correlation coefficients were all greater than 0.999, and the detection limits was between 0.000 7-0.089 μg·g-1. The recovery rate of ten elements was between 93% and 110%, and the relative standard deviation was between 0.30% and 1.69%. Conclusion: This method is fast, simple and accurate. It can provide a scientific basis for the comprehensive utilization of resources.关键词：ICP-AES;Prunus mongolica;determination of elements
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发布时间：2024-01-04 - 摘要：Objective:To study the influence of different processed methods on the content of 6-gingerol in ginger. Method: HPLC was used to determine 6-gingerol in ginger processed by fried yellow, fried carbon (clean fry, stewed calcined), wheat bran fry, and sand fry respectively. Result: The content of 6-gingerol from high to low was fried yellow, wheat bran fry, crude ginger, sand fry, fried carbon (clean fry), stewed calcined. Conclusion: The amount and timing of heat in processing procedure were the main factors affecting the content of 6-gingerol in ginger.The temperature of fried yellow was lower than wheat bran fry, however the content of 6-gingerol in fried yellow ginger was higher than that in wheat bran fry Ginger with 8.57 mg·g-1 and 8.14 mg·g-1 respectively. A certain degree of heat can improve the content of 6-gingerol in ginger.关键词：Rhizoma Zingiber;processing Rhizoma Zingiber;6-gingerol;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC method for determination of acteoside and luteolin in Callicarpa nudiflora. Method: HPLC method was applied on Phenomenex Gemini C18 column (4.6 mm×250 mm, 5 μm) at 30℃ under the condition of gradient elution with mobile A (methanol) and mobile B (0.3% phosphate). The flow rate was 1.0 mL·min-1, the detection wavelength was set at 350 nm. Result: Two components had a good liner relationship in concentration of 29.6-740.0, 1.0-25.0 μg (r=0.999 9). The average recoveries were 99.08%, 99.93% and the RSD was 1.53%, 1.65% respectively. Conclusion: The method is accurate and reproducible for determination of acteoside and luteolin in C. nudiflora.关键词：Callicarpa nudiflora;acteoside;luteolin;HPLC;determination
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC-ELSD method for simultaneous quantitation of six triterpene acids from Eriobotrya japonica. Method: Samples were analyzed with Ultimate XB C18 column (4.6 mm×250 mm,5 μm) and eluted with acetonitrile-methanol-0.5% ammonium acetate solution as mobile phases in gradient mode,flow rate was 1.0 mL·min-1,column temperature of 35℃,drift tube temperature of ELSD was set at 95℃,and flow rate of nitrogen was 2.5 L·min-1. Result: There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0.436-2.180,0.392-1.960,0.39-1.960,0.856-4.280,0.424-2.120,1.592-7.960 μg for rosolic acid,tormentic acid,maslinic acid,corosolic acid,oleanolic acid,ursolic acid,respectively.The average recoveries of these six triterpene acids were 98.9%(RSD 3.8%),96.3%(RSD 2.7%),97.7%(RSD 2.6%),97.6%(RSD 3.2%),96.6%(RSD 1.9%),102.5%(RSD 2.5%),respectively. Conclusion: This established method was simple,accurate and repeatable with good separation,it could provide experimental basis for a better quality control of E. japonica.关键词：Eriobotrya japonica;rosolic acid;tormentic acid;maslinic acid;corosolic acid;oleanolic acid;ursolic acid
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发布时间：2024-01-04 - 摘要：Objective:To describe a method for determining of salidroside and paeoniflorin in compound Qitian capsules by HPLC. Method: The determination was carried out over a COSMOSIL column (4.6 mm×250 mm, 5 μm) at the temperature (35℃). The mobile phase consisted of methanol (A)-0.02％ phosphoric (B) solution with the gradient elution (0-15 min, 19%; 15-50 min, 28%A; 50-60 min, 28%A, the flow rate:1 mL·min-1 and the detection wavelength was set at 275 nm during 0-25 min and 230 nm during 26-60 min. Result: Two regression equation showed good linear relationship between the peak area ratio of each marker to internal standard and amounts. The average recovery of salidroside was 98.33% (RSD 0.71%), of paeoniflorin was 97.77% (RSD 1.18%). Conclusion: The method is simple, accurate, sensitive, and can be used for determination of two components in Qitian capsules with satisfactory accuracy and repeatability.关键词：Qitian capsules;HPLC;salidroside;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective:To compare the changes of chlorogenic acid and luteolin content in sulfur fumigation and drying Lonicera japonica, in order to study the chemical components of L. japonica. Method: The content of chlorogenic acid and luteolin from different origin and different processing methods was determined with HPLC. Result: Chlorogenic acid was significantly increased, while the content of luteolin changed very little after sulfur smoked. Conclusion: The content of chlorogenic acid has increased significantly after sulfur smoked, but the content of luteolin almost had no effect. The content of two components from different areas was changed very widely.关键词：HPLC;sulfur;drying;Lonicera japonica;chlorogenic acid;luteolin;comparison
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发布时间：2024-01-04 - 摘要：Objective:To analyze and identify the chemical components and compare the chemical constituents of volatile oil from leaves and stems of Glycosmis parviflora, providing scientific basis for its further research. Method: The essential oil was extracted by steam distillation. The constituents were separated and identified by GC-MS. The relative content of each constituent was determined by area normalization method. Result: 38 and 36 compounds were identified respectively in the volatile compounds of leaves and stems, accounted for 92.28% and 80.57% of total essential oil. Conclusion: The chemical constituents of volatile oils were different between the leaves and stems, but the relative content of caryophyllene was the highest in the essential oil both.
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发布时间：2024-01-04 - 摘要：Objective:To establish the quality control method and study the stability of Ganju dropping pills at accelerated testing and room temperature. Method: Radix Glycyrrhizae and Inula cappa in the pills were identified by TLC. The content of glycyrrhizic acid was determined by HPLC. Result: After the accelerated testing and the long-term testing, various indexes of 3 pilot test samples accorded with the quality standard of Ganju dropping pills. Conclusion: Ganju dropping pills are stable and controllable in quality, excellent in stability.关键词：Ganju dropping pills;stability;TLC;HPLC;glycyrrhizic acid
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发布时间：2024-01-04 - 摘要：Objective:To establish UPLC determination method of Phragmitis Rhizoma in Baiyangdian. Method: Ten samples of Phragmitis Rhizoma in Baiyangdian from different place were extracted by 70% methanol with ultrasonic extractor. Sample solutions were determined by Waters UPLC equipped with BEH C18 column and PDA detector, gradient eluted with acetonitrile-water as mobile phase. The flow rate was set to 0.1 mL·min-1, while the column temperature was set at 25℃, and the wavelength for detection was set to 320 nm. Result: The standard curve was in good linearity over the range of 0.038-0.38 μg (R2=0.999 9). Conclusion: The method established is simple, accurate and can be used to control the quality of Pharagmitis Rhizoma producted in Baiyangdian.
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发布时间：2024-01-04 - 摘要：Objective:To establish the quantitative analysis method for determining the content of gallic acid in Sanguisorba Granule by HPLC so as to provide a scientific basis for development of the drug as well as establishment of its quality standards. Method: Diamonsil C18(2) column (4.6 mm×250 mm,5 μm) was used with methanol-0.05% phosphoric acid (5:95) as the mobile phase; the detection wavelength was 272 nm, the velocity of flow was 1.0mL·min-1, and the column temperature was 20℃. Result: Under the above chromatographic conditions, the injection volume of gallic acid showed a linear relationship with peak area when ranging between 0.009 72-0.097 2 mg·kg-1, the corresponding regression equation being Y=34 347.2X+10.887 (r=0.999 9); the average recovery of gallic acid was 99.83% with corresponding relative standard deviation (RSD) being 2.3% (n=6). Conclusion: The method is simple, accurate, reproducible and can be used for determination of the content of gallic acid in Sanguisorba Granule.关键词：Sanguisorba Granule;gallic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To develop a high performance liquid chromatographic method for simultaneous determination of myricetin and quercetin in Rose roxburghii. Method: The optimized method was achieved for the separation and detection of myricetin and quercetin using Zorbax SB-C18 (4.6 mm×150 mm, 5 μm) as the stationary phase, methanol-0.4% aqueous phosphoric acid solution (65:35) as the mobile phase at a flow rate of 1.0 mL·min-1, 368 nm as the detection wavelength. Result: Myricetin and quercetin showed good relationship in the range of 0.036 2-0.253 7 μg and 0.056-0.196 μg, respectively. The average recoveries of myricetin and quercetin were 98.32% and 98.84% with relative standard derivation (RSD) of 2.18% and 1.78%. Conclusion: The results showed that the method is simple, accurate and repeatable and it is suitable for quality control of R. roxburghii.关键词：high performance liquid chromatography;Rose roxburghii;myricetin;quercetin
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发布时间：2024-01-04 - 摘要：Objective:To improve the quality standard of Wanbiqing Pill. Method: The TLC method was used to identify Arnebiae Radix, Euodiae Fructus and Smilacis Glabrae Rhizoma. The HPLC method was used to detect the content of loganin in Lonicerae japonicae caulis. Result: This TLC method is good in separation, strong in specificity and negative in interference. Loganin was detected in the range of 0.013 4-0.080 4 g·L-1, indicating good linear relationship (r=0.999 8). The mean sample recovery rate was 97.07% and the RSD was 1.24%. Conclusion: The TLC and HPLC methods are simple and accurate in operation, high precision and good in reproducibility, and they can be taken as the standard of quality control for Wanbiqing Pill.关键词：Wanbiqing Pill;TLC;HPLC;loganin
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the determination of volatile oil and the determination of eucalyptol in volatile oil as an indicator of Magnoliae Flos (Magnolia biondii) by GC. Method: The content of volatile oil of the mainstream 10 cultivars of M. biondii was determined by determination of volatile oil from the 2010 edition of Chinese Pharmacopoeia, and the content of eucalyptol of the mainstream 10 cultivars was determined by GC. The GC-MS conditions with HP-5MS column (0.25 μm×0.32 mm×30 m) was used, FID detector, the hold air consisted of high purity He, temperature programmed: initial temperature 80℃, retain 6 min, rise temperature to 160℃ at the rate of 20℃·min-1, retain 2 min; rise temperature to 220℃ at the rate of 60℃·min-1, retain 5 min; inlet temperature 240℃; detector temperature 240℃. Result: The content of volatile oil: 3.24%,4.64%,6.12%,3.64%,5.48%,6.98%,3.64%,3.24%,7.68%,2.88%. Content of eucalyptol determined by GC: the linear range was 1.1-10.45 μg(r=0.999 8); the average recovery was 100.02%,RSD 1.12%. The content of volatile oil: 2.076%,4.212%,3.689%,0.1041%,1.590%,3.523%,2.930%,1.940%,4.520%,1.494%. Conclusion: According to the volatile oil content and the content of eucalyptol determined by GC, there were differences in different cultivars of M. biondii, which provided scientific basis for the quality assessment and quality control standards of different cultivated species of M. biondii.关键词：Magnoliae Flos;Magnolia biondii;different cultivated species;content determination of volatile oil;gas chromatography;eucalyptol
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发布时间：2024-01-04 - 摘要：Objective:To develop a new method for simultaneous determination of daucosterol and β-sitosterol in Alysicarpus vaginalis. Method: HPLC-ELSD method was used to determine daucosterol and β-sitosterol. The separation was performed on a Diamonsil C18 column (4.6 mm×150 mm, 5 μm) with methanol-water (95:5) as the mobile phase at the flow rate of 1.0 mL·min-1. The temperature in drift tube was 80℃ and the carrier gas flow was 2.5 L·min-1. Result: The linear ranges of daucosterol and β-sitosterol were 10-100 μg·mL-1 (r=0.999 3) and 25-250 mg·L-1 (r=0.999 1), respectively. The average recoveries were 99.5%, 98.9%, and RSDs were 2.4% and 2.1%, respectively. Conclusion: This method is selective, simple, accurate, which can be applied for simultaneous determination of daucosterol and β-sitosterol in A. vaginalis.关键词：Alysicarpus vaginalis;daucostero;β-sitosterol;HPLC-ELSD
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发布时间：2024-01-04 - 摘要：Objective:To establish the content determination method for ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii. Method: HPLC method was adopted. The chromatographic column was Diamonsil C18 (4.6 mm×250 mm, 5 μm) column. The mobile phases were acetonitrile-0.1% phosphate solution (gradient elution) and methanol-0.1% phosphate solution (3:97) respectively. The detection wavelengths were set at 203 nm and 254 nm. Result: The concentration have good linear correlations within the range of 0.050 4-1.26 μg for notoginsenoside Rg1, 0.336-8.4 μg for ginsenoside Re, 1.052-26.3 μg for notoginsenoside Rb1 and 0.806 4-20.16 μg for vitamin C. The average recovery rates were 98.59%, 99.11%, 98.12% and 97.50% respectively. Conclusion: The method is simple, reproducible and accurate, and applicable for the quality control of the product.关键词：compound effervescent tablets of Radix Panacis Quinquefolii;HPLC;ginsenoside;vitamin C
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发布时间：2024-01-04 - 摘要：Objective:To develop an ultra performance liquid chromatography (UPLC) method for fingerprints of Jinlida granule. Method: The Waters Acquity UPLC BEH C18(2.1 mm×100 mm, 1.7 μm) column was used. Acetonitrile and water containing 0.1% phosphoric acid were used as mobile phase with gradient elution. The detection wavelength was set at 203 nm. Flow rate was set at 0.3 mL·min-1 and column temperature was maintained at 40℃. Result: UPLC fingerprint was established with 50 common peaks, nine of 50 common peaks were identified, and the similar degrees of the UPLC fingerprints of Jinlida granule from 10 batches were between 0.991 and 1.000. Conclusion: The method of fingerprints has acceptable precision, reproducibility and stability, which can be used as a new means for the quality control of Jinlida granule.关键词：UPLC;fingerprints;Jinlida granule
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发布时间：2024-01-04 - 摘要：Objective:To develop a method of determination of trace elements in Fructus Forsythiae. Method: The content of Cu, Zn, Mn, Fe, Ca, Mg, K, Na in Fructus Forsythiae was directly determined by flame atomic absorption spectrophotometry after digestion with HNO3-HClO4(4:1) at heating and normal pressure. Result: All calibration curves showed good linearity (r>0.990 6). The RSD (n=6) of reproducibility was between 1.59% and 2.98%. The recoveries (n=6) were from 96.2% to 106.6%. The RSD (n=6) of recovery was between 1.4% and 3.9%. Conclusion: The method was simple, accurate and sensitivity. It is a reliable method for the determination of the trace elements in Fructus Forsythiae.关键词：FAAS;Fructus forsythiae;trace elements
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发布时间：2024-01-04 - 摘要：Objective:To establish a method of flavone and organic acid compounds by HPLC fingerprint, providing the basis for whole control and evaluating the quality of medicinal materials. Method: The separation was performed on an BDS C18(4.6 mm×250 mm, 5 μm) column of Hypersil. The mobile phase was methanol-0.1% phosphoric acid solution, gradient elution. The flow rate was 1.0 mL·min-1 and the wavelength was set at 330 nm. Result: HPLC fingerprint of flavone and organic acid compounds was established. found 17 co-possessing peaks, RSD of precision and reproducibility was in the range of<5%, and the similarity of 13 batches of Glechoma longituba (Nakai) Kupr was between 0.713-0.987. Conclusion: This method is simple and accurate, reproducible and can be used to provide a base for the quality of the herbs.关键词：Glechoma longituba;flavone;organic acid;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: Using reversed-phase high performance liquid chromatography (RP-HPLC) method to analyze 6 ingredients of Zhitong prescription one-time. Method: Using Diamond C18 column (4.6 mm×250 mm, 5 μm), the column temperature was 30℃; methanol acetonitrile 0.1% ammonia water as the mobile phase, 1 mL·min-1 gradient elution; detection wavelength was 230 nm (paeoniflorin), 235 nm (the aconitine hypaconitine), 280 nm (tetrahydropalmatine), 280 nm (protopine) and 235 nm (mesaconitine). Result: The peak area and concentration of the six components showed good linearity (r>0.999 4) the paeoniflorin aconitine hypaconitine, tetrahydropalmatine protopine, new aconitine the recoveries were 97.2% (RSD 1.4%), 96.5% (RSD 1.2%), 102.5% (RSD 0.7%), 96.8%(RSD 0.7%), 100.8%(RSD 1.1%), 98.5%(RSD 1.1%). Conclusion: By using RP-HPLC simultaneous determination of the active ingredients of the the analgesic prescription of several simple, we can provide a basis for the quality control of the preparation of Zhitong prescription.关键词：high performance liquid chromatography;analgesic side;ingredients
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发布时间：2024-01-04 - 摘要：Objective:To study on the purification and chemical composition of polysaccharides from Chrysanthemum indicum. Method:Polysaccharides were extracted from C. indicum with hot water, followed by precipitating with ethanol, decolorizing with LSA-21 and removing of free protein by the method of Sevag, and finally separated into three fractions CIP-1'、CIP-2'and CIP-3'by DEAE-52 cellulose anion exchange and Sephadex G75 gel column chromatograph. Result:The relative molecular weight (Mw) of CIP-1'was estimated to be about 8,242 Da by using high-performance gel permeation chromatography (HPGPC),while that of CIP-2'was 8,383 Da and of CIP-3'was 1.920 1×103 Da. According to GC analysis, CIP-1'was composed of six kinds of monosaccharides, namely rhamnose, arabinose, xylose, mannose, glucose and galactose in the molar ratio of 5.44:16.40:20.35:3.53:15.43:21.46, following that CIP-2'was composed of the same kinds of monosaccharides in the molar ration of 8.47:13.17:2.43:2.75:14.51:15.76. However, CIP-3'was composed of rhamnose, arabinose, mannose, glucose and galactose in a molar ratio of 17.46:15.88:18.90:63.76:36.44. The FT- IR spectrum of the three also indicated typical characteristics of polysaccharides. Conclusion:This study provided experimental basis for the development of polysaccharide resources from C. indicum.关键词：Chrysanthemum indicum;polysaccharide;HPGPC;GC;IR;chemical composition
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发布时间：2024-01-04 - 摘要：Objective:To investigate the chemical compositions of the leaves of Syringa oblata. Method: The separation was performed by using silica gel column chromatography, recrystallization and the HPLC preparation methods. The compound structures were identified according to the spectral analytical methods UV, IR,1H-NMR,13C-NMR, HSQC and HMBC. Result: The structures of the nine compounds were fully determined by the chemical and physicochemical evidences. They were (+)pinoresinol-4″-O-β-D-glucopyranoside(1). lariciresinol-4-O-β-D-glucopyranoside (2). (+)epipinoresinol-4-O-β-D-glucopyranoside (3). 3,4: 3',4'-bis(methylene-dioxy)-9'-hydroxyl-lignane-9-methyl-O-β-D-glucopyranoside (4). 2-(3,4-dihydroxyphenyl) ethyl (1R,4aS,8R,8aS)-8-methyl-6-oxo-1-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethy)oxan-2-yl]oxy-4a,5,8,8a-tetrahydro-1H-pyrano[3, 4-c]pyran-4-carboxylate(5). 3,4-dihydroxybenzene-Styrene glycol (6). 4-hydroxyphenethyl alcohol (7). 6-O-(E)-feruloyl-(α)-glucopyr-anoside (8). 6-O-(E)-feruloyl-(β)-glucopyranoside (9). Conclusion: All of the constituents are obtained in leaves of S. oblata; among them, compounds 1-6 are reported in this species for the first time.关键词：Syringa oblata;chemical constituents;lignan glycosides;iridoid glycoside
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发布时间：2024-01-04 - 摘要：Objective:To analyze the volatile constituents in Cardamine urbaniana. Method: The volatile oil from C. urbaniana was extracted by steam distillation (SD) and solid phase micro extraction (SPME). The constituents were separated and analyzed by gas chromatography-mass spectrometry with the area normalization method to determine the relative percentage content of each component. Result: Sixty-seven compounds were isolated and 34 compounds were identified by SD that composed about 94.34% of the total volatile oil; and 62 compounds were isolated and 48 compounds were identified by SPME that composed about 98.29% of the volatile oil. Five kinds of the volatile components are identified by the two methods together and the content of 4-isothiocyanato-1-butene is highest. Conclusion: SPME and SD were applied to the extraction of different types of volatile components, combined with the two methods together to analyze the volatile components in C. urbaniana can get more comprehensive information.关键词：SD;HS-SPME;GC-MS;Cardamine urbaniana;volatile constituents
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发布时间：2024-01-04 - 摘要：Objective:To establish chromatographic fingerprint of volatile oil and investigate the variance of Rhizoma Zingiberis collected from different places. Method: First, gather Rhizoma Zingiberis from different places, then smashed and dried out. Second, mark off the different samples and extract the volatile constituents. Third, assay the final samples by GC and correct their fingerprints and make evaluations at last. The chromatography analysis was carried out on an HP-5 capillary column (320 μm×30 m,0.25 μm), the temperature program was as follows: 60℃ for six minutes, rose to 146℃ at 5℃·min-1, then rose to 165℃ at 2℃·min-1 for five minutes, and then rose to 250℃ at 8℃·min-1, by using helium as the carries gas, the flow rate of 1 mL·min-1, the split ratio 30: 1, sample inlet temperature of 250℃. Result: A total of twenty common peaks identified from the GC fingerprints of volatile oil in Rhizoma Zingiberis.More than eleven samples of representative Rhizoma Zingiberis were chosen for preliminary fingerprint assay. The similarity, repetitiveness, steadiness and precision of these different Rhizoma Zingiberis were obtained. Conclusion: The quality of most of these Rhizoma Zingiberis is stable and controllable, which meet the requirements of the 2005-edition Pharmacopoeia. However, a minority of these Rhizoma Zingiberis has some differences in quality, and requires further investigation.关键词：Rhizoma Zingiberis;volatile oil;fingerprint atlas;GC
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发布时间：2024-01-04 - 摘要：Objective:To study the absorption, distribution and excretion of hyperoside after intragastric administration to rats. Method: Hyperoside was fed to rats by intragastric and intravenous administration, respectively. Plasma, tissues and excretion samples were collected at different time. The samples were analyzed by HPLC for the detection of quercetin after enzymatic hydrolysis treatment with combined β-glucuronidase and sulphatase, using kaempferol as internal standard. Result: After intragastric administration of hyperoside (12.5, 25, 50 mg·kg-1) to rats, the main pharmacokinetic parameters t1/2e was (3.47±0.76), (3.52±0.87), (4.17±1.02) h-1, respectively; tmax was 0.5 h; Cmax was (0.528±0.230), (1.136±0.451), (2.033±1.147) mg·L-1, respectively; AUC was (2.67±0.28), (4.11±0.37), (6.72±0.83) mg·L-1·h-1, respectively. Based on the weighted regression analysis of the pooled data, Cmax and AUC values increased as the dose increased in a linear manner (r was 0.998 and 0.999, respectively), consistent with the linear pharmacokinetics. As compared to the intravenous administration at a single dose of (25 mg·kg-1), the absolute bioavailability after intragastric administration of hyperoside was estimated to be 26.0%. After intragastric administration to rats, the rank order of normalized tissue distribution was stomach>intestines>kidney, liver, muscle, lung, heart>cerebrum, uterus>spleen, testis>fat. After intragastric administration hyperoside (25 mg·kg-1), excretion amount was only (0.71±0.13)% from urine and (2.04±0.36)% from faeces during 72 h, only (1.56±0.22)% from bile during 24 h, respectively. Conclusion: Hyperoside was rapidly absorbed in rats with a long t1/2e. There was no accumulation in blood and main tissues of hyperoside. The main excretion way of hyperoside was not prototype or glucoside forms from urine, faeces or bile excretion.关键词：hyperoside;pharmacokinetics;absorption;distribution;excretion
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发布时间：2024-01-04 - 摘要：Objective:To study the regulation of Cinnabar, Zhusha Anshen wan(ZSASW) and HgS on absorption and distribution in mice. Method: Oral gavage was given to Kunming mice with cinnabar (0.2 g·kg-1), high dose of cinnabar (11.2 g·kg-1), ZSASW (2 g·kg-1), HgS (0.07 g·kg-1) or saline daily for 1, 2, 4, 8, 16, 24 hours, mercury accumulation in blood, liver, kidney and brain was determined by atomic fluoresence detector. Result: In comparison, the maximal serum concentration and serum concentration in each time of cinnabar clinical dose group and ZSASW group have obvious difference with the HgCl2 group. The mercury accumulation in liver and kidney in each time of clinical dose group and ZSASW group have obvious difference with the HgCl2 group. The mercury accumulation in blood and organs also had obvious difference between cinnabar clinical dose group and high dose group, and the high dose has the maximal accumulation in brain. Conclusion: Both cinnabar clinical dose group and ZSASW group have obvious difference with cinnabar high dose group and HgS group in absorption and mercury accumulation in organs.关键词：cinnabar;Zhusha Anshen wan;HgS;absorption;distribution
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发布时间：2024-01-04 - 摘要：Objective:To obtain chemical constituent information of rat serum after oral administration of Rhizoma Corydalis extraction. Method: ABI 4000 Q TRAP Mass was applied to determine the serum of rat and the compounds were analyzed with mass combined with references and standards. Result: Eight compounds in rat serum were identified. Conclusion: They are tetrahydropalmatine, dehydrocorydaline, tetrahydrocoptisine, corypalmine, isocorypalmine, berberine, yuanhunine and palmatine/dehydrocorybulbine.关键词：Rhizoma Corydalis;qualitative analysis;LC-MS-MS
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发布时间：2024-01-04 - 摘要：Objective: The factorial design method of effective parts of Astragalus 2×2×2 (Astragalus flavonoids, polysaccharides of astragalus and Astragalus saponin) was used analyze intervention of effect and mechanism in hemorrhagic anemia model mice and their interaction. Method: One hundred and eight mouse randomly were divided into 9 groups, 12 each group. In addition to normal group, orbital venous bleeding was executed at 1, 4,7 day, rasprctively, bloodletting is 0.5 mL·20 g-1, normal groups, model groups and intragastric infusion were given with distilled water 10 g·kg-1, other groups were given corresponding drugs with dose of 0.166, 0.445, 0.611, 0.069, 0.235, 0.514, 0.680 g·kg-1. Factorial design analysis of variance methods was used to analyze peripheral hemoglobin (Hb) concentration, red blood cell (RBC) count, hematocrit (Hct), hematopoietic factors such as indicators for analysis of variance. Result: Compared with normal group, peripheral blood Hb, RBC, Hct, erythropoietin(EPO), serum transcription factor(GATA-1) in serum and kidney tissue, bone marrow, such as stem cell factor(SCF) index differences are statistically significant in model group(P<0.05). Flavone,polysaccharide and saponins glycoside had an obvious effect on peripheral blood (P<0.05), combined application of flavone and saponins glycoside and polysaccharide and saponins glycoside showed a better action than single medication; flavone and polysaccharide had influence on serum EPO, GATA-1 and bone marrow SCF concentration, polysaccharide and saponins glycoside had effect on kidney organization EPO concentration. Conclusion: Combination of polysaccharides and saponins can improve peripheral blood in anemia mice picture.关键词：factorial design;effective parts of Astragalus;hemorrhagic anemia model;interaction relationship
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发布时间：2024-01-04 - 摘要：Objective:To explore possible molecular mechanism of Tangluoning(TLN) formula in improving oxidative stress. Method: The SD rats were divided into control group, the others were given intraperitoneal injection of streptozocin(STZ) (60 mg·kg-1) after 12 hours of fasting to build diabetic model. The rats with blood glucose level being more than 16.7 mmol·L-1 after stop 8 hours of fasting were regarded as modelling successfully after 3 days.The rats modeling successfully were randomly divided into the model group, TLN group and alpha-lipoic acid group (LA), 15 in each group. Drugs were administrated orally for 8 weeks after the STZ treatment.Blood glucose, cool or thermal response time,thermal threshold were determined during the experimental process. After eight weeks of treatment,Real-time PCR technology was employed to measure Mn superoxide dismutase(SOD) mRNA,CuZnSOD mRNA and glutathione peroxidase(GPx) mRNA expression in the sciatic nerve. Result: The expression of MnSOD mRNA,CuZnSOD mRNA and GPx mRNA was increased after STZ treatment. Compared with the model group,the cool or thermal response time was decreased, the thermal threshold was increased, the gene expression of MnSOD,CuZnSOD and GPx was increased by TLN. Conclusion: Tangluoning formula could improve hyperalgesia, dysesthesia,increase antioxidant enzymes gene expression to improve oxidative stress.关键词：diabetic peripheral neuropathy;MnSOD mRNA;CuZnSOD mRNA;GPx mRNA;Tangluoning formula;oxidative stress.
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发布时间：2024-01-04 - 摘要：Objective:To study the inhibition of fatty acid synthase (FAS) by Xuezhiling tablets in vitro, and explore its mechanism of action. Method: The ultraviolet spectroscopy was used to evaluate the activity of FAS through monitoring the alteration of absorbance(A) value of reduced nicotinamide-adenine dinucleotide phosphate(NADPH) and the substrates were respectively acetyl coenzyme A(AcCoA), malonyl-CoA(MalCoA), acetoacetyl-coenzymeA(AcAcCoA), ethyl acetoacetate, ethyl crotonate, NADPH for different active sites. The inhibition of FAS by overall reduction and different active sites was separately detected after the treatment with different doses of Xuezhiling tablets. Result: The activity of FAS could be inhibited by Xuezhiling tablets. After the treatment with Xuezhiling tablets (150 mg·L-1), the inhibition ratio was 51% with manner if time and dose relationship. For the different active sites of FAS, Xuezhiling tablets displayed different abilities. It showed more potential in inhibiting the enoyl reduction and AcAcCoA reduction than keto-acyl reduction, and it was also dose dependent for the different active sites. The residual activity of different active sites was less than 70%,and more than 50%, when FAS was treated with Xuezhiling tablets at the concentration of 150 mg·L-1. Conclusion: FAS can be inhibited by Xuezhiling tablets, and this ability was attributed to inhibit the different active sites of FAS. This article prove that the ratiocination about the lowering blood lipids of Xuezhiling tablets is related to FAS and it can be a reference for Xuezhiling tablets used for obesity and other disease that related to FAS.关键词：Xuezhiling tablets;fatty acid synthase (FAS);inhibitory action;inhibition mechanisms;lowering blood fat
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发布时间：2024-01-04 - 摘要：Objective:To establish the characteristic Chart of Xiaobanxia plus Fuling decoction to find the basic substances of its antiemetic effects. Method: HPLC was used to establish the characteristic chart of Xiaobanxia plus Fuling decoction. Fifty pigeons were divided into several groups, treated with salt water or different dosage of Xiaobanxia plus Fuling decoction, Xiaobanxia decoction and Rhizoma Pinelliae. Pigeon vomiting models were estabished by copper sulphate. The latency period of vomiting, vomiting times and frequency were observed to study the antiemetic effect of Xiaobanxia plus Fuling decoction extract and the separating prescription(pinellia temate and ginger, pinellia temate) aqueous extract. Result: The content of ginger phenols in the original decoction was the highest, and among which 6-gingerol was the highest. Both the original decoction and its separating prescription had different degree of antiemetic effect. Xiaobanxia plus Fuling decoction>Xiaobanxia decoction>Rhizoma Pinelliae. Conclusion: Xiaobanxia plus Fuling decoction and its separating decoction have certain antiemetic effect. Considering the chart obtained form HPLC ginger phenols is the major content, which suggests that gingerol may be one of the major basis substance for its antiemetic effect in this prescription.关键词：Xiaobanxia plus Fuling decoction;separating prescription;antiemetic effect;gingerol;characteristic spectrum
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发布时间：2024-01-04 - 摘要：Objective:To probe the intervention effects of Jiawei Wendan decoction on intracellular calciumion concentration of hippocampal neuron in rat depression model. Method: A total of 196 Sptague-Dawley rats were equally and randomly assigned to four group:blank control, model, traditional Chinese medicine treatment group,and western medicine treatment group.Depression model was established,exception for the blank group,by independent feeding and chronic stress for 21 days.In addition,traditional Chinese medicine and western medicine treatment were administered by Jiawei Wendan decoction 12 g·kg-1·d-1and fluoxetine 1.8 mg·kg-1·d-1,respectively.Model and control group were treated with saline for 28 days. At the 7th, 14th, 21st, 28th day of the experiment, we detected changes of Ca2+ concentration in hippocampus neuron of each group by confocal laser technology. Result: During the progress of depression, the concentration of Ca2+ in depressive rat's hippocampus became higher and higher. At the 21st and 28th day, fluorescence intensity of Ca2+in the model control group was higher than that of the blank group. After administration, this situation had been improved that fluorescence intensity of Ca2+in Jiawei Wendan decoction-treated group and fluoxetine-treated group was both lower than that of the model control group at the 21st and 28th day(P<0.05 or P<0.01). Conclusion: During the progress of depression, the concentration of Ca2+ increase obviously. Excessive influx of Ca2+ in hippocampus neuron can be inhibited by giving Jiawei Wendan decoction as to improve neuroplasticity for producing antidepressive effect.关键词：depression;Jiawei Wendan decoction;hippocampus;Ca2+;laser scanning confocal microscope
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发布时间：2024-01-04 - 摘要：Objective:To optimize the processing technology of Hanshuishi and obtain the basic pharmacodynamic parameters. Method: An orthogonal experiment of four factors and three levels was conducted to evaluate the Hanshuishi processing technology, and the characters of calcined products and CaCO3 content were used as observation indexes. The animals were divided into model group, 100 mg·kg-1 Ranitidine group, 360.4 mg·kg-1Jiebai Wan group, 334.3 mg·kg-1the Jiebai Wan removed Hanshuishi group, 26.1 mg·kg-1 henan Hanshuishi, zejiang Hanshuishi and xinjiang Hanshuishi groups, Pylorus-ligated rat model was applied to observe the effect on gastric juice volume, acidity and pH value of the rats subjected to intragastric administration. Result: The processed traits of Hanshuishi were crisp, white color and powder. The optimum conditions of the Hanshuishi were particle size of powder (20 mesh), temperature of 1 000℃, time 1.5 h; In addition to the 13th sample produced in Zhejiang, the other Hanshuishi groups and the Jiebai Wan group removed Hanshuishi could significantly decrease the volume, total acidity and 1 h acid excretion of gastric juice of experimental rats and increase pH value; Except of increasing the pH value, Jiebai Wan group had no effect, The efficacy of henan Hanshuishi was optimal and the Zejiang one had a poor performance. Conclusion: Hanshuishi inhibited gastric secretion of rats; moreover, the efficacy of Hanshuishi from different areas has slightly different.关键词：Hanshuishi;processing;pharmacodynamics
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the effect of Euphorbia fischeriana extract (EFE) on metastatic ability of melanoma B16 cells in vivo. Method: The B16 mouse transplanted tumor model and B16 spontaneous motility melanoma metastatic model were established, and C57BL/6 mice were randomly divided intocontrol group, group EFE 15.0,20.0 g·kg-1 and cyclophosphamide (CTX) group to detect the inhibition effect of EFE for growth of transplanted tumors and metastatic incidence on lung. The expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) and protein kinase B(p-Akt) of transplanted tumors were observed by immunohistochemical method. Result: EFE could inhibit the growth of B16 cells and the lung metastasis significantly in vivo. The growth inhibition rate of EFE when injected intrapetitoneally to mice at concentration of 15.0, 20.0 g·kg-1 were 52.9% and 63.6% respectively (cyclophosphamide was 59.5%); inhibition rate of lung metastasis were 37.4% and 63.5% (inhibiton rate of cyclophosphamide was 56.8%). Immunohistochemical results indicated that compared with control group, expression of p-Akt in EFE group was less apparent while PTEN expression was more visible than control group. Conclusion: EFE could inhibit the metastasis of B16 cells in vivo, probably through down-regulating of p-Akt and up-regulating of PTEN expression, and further study are warranted.关键词：Euphorbia fischeriana extrat;melanoma;B16 cells;metastasis
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发布时间：2024-01-04 - 摘要：Objective:To investigate the change of immunologic function in deficiency cold model induced by hydrocortisone. Method: Forty eight male SD rats were divided into two groups. The control group was intramuscularly injected saline 0.25 mL, the experimental group was intramuscularly injected hydrocortisone sodium succinate saline 20 mg·kg-1. 10 μL blood sample was collected from tail on 7th day, 14th day and 21th day.WBC count was detected by the microscope. 5 mL blood sample was collected from abdominal main vein on 15th day and 22th day. Serum IgG and IgM levels were detected by automatic biochemical analyzer. Plasma interleukin-4(IL-4) and interferon-γ(IFN-γ) levels were determinated by elisa. Then thymus and spleen were removed and weighed to calculate the organ index. Result: Compared with the control group, the thymus index and serum IgG of experimental group were significantly lower on 14th day and 21th day(P<0.01,P<0.05), the spleen index and serum IgM were significantly lower on 14th day(P<0.05)and no change on 21th day, the WBC count were significantly lower on 7th day, 14th day and 21th day(P<0.01, P<0.05), plasma IL-4 was low(P<0.05), IFN-γ level was high(P<0.05) and IFN-γ/ IL-4 was high(P<0.05)on 21th day. Conclusion: During the experimental process, the immunologic function was significantly lower, and associated with shift of Th1/Th2 in deficiency cold status.关键词：deficiency cold status;immune system;hydrocortisone
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发布时间：2024-01-04 - 摘要：Objective:To observe the effects of ethyl acetate extraction from Gastrodia elata on the neuroplasticity of rat cerebral ischemia injury model. Method: Sprague-Dawley rats were randomly divided into sham-operation group,model group and ethyl acetate extraction from G. elata group(ethyl acetate extraction group, 7.29 g·kg-1). The rat models of middle cerebral artery occlusion were replicated 0.5 h later after the last dose of continuous oral administration of drug on the 7th day and keep administration of drug for another 7 days. Statistic data were collected on death rate of model on 7th day,as well as neurological behavior and expression of GAP-43 at specified time point. Result: Compared with the sham-operation group, the death rate and neurological syndromes of model group were markedly increased and the hanging wire and expression of GAP-43 were significantly decreased. Compared with the model group, the death rate and neurological syndromes of the ethyl acetate extraction group were significantly decreased and the hanging wire and expression of GAP-43 were significantly increased (P<0.05,P<0.01). Conclusion: The results suggest that the ethyl acetate extraction from G. elata can improve the neuroplasticity of cerebral ischemia injury model rat.关键词：ethyl acetate extraction from Gastrodia elata;death rate;neurological syndromes;hanging wire;expression of GAP-43
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发布时间：2024-01-04 - 摘要：Objective:To observe the impact of Scropio-Scolopendra on airway inflammation and airway remodeling of asthmatic rats, and to explore the mechanism of action of Scropio-Scolopendra for asthma. Method: Thirty SD rats were randomly divided into three groups:normal control group(group A),model control group(group B), Scropio-Scolopendra group(group C),10 in each group.The asthmatic rat airway remodeling model was established by ovalbumin sensitized method, The rats in C group was administrated Scropio-Scolopendra by ig(0.625 g·kg-1·d-1),and in A,B group rats were administrated the equal physiological saline in the same way. After three weeks of treatment, the number and the percentage of eosinophil(E),macrophage(M),lymphocyte(L),neutrophilic in bronchoalveolar lavage fluid(BALF).Pulmonary pathological changes were observed by HE stain and Masson stain.The thickness of bronchial wall (Wat/Pbm),smooth muscle(Wam/Pbm) and collagen fibers(Wac/Pbm)were determined in medical image analysis software. Result: :Compared with normal group,number of total leukocytes and the percentage of eosinophil,neutrophil,lymphocyte in BALF were significantly higher in the model group(P<0.01). The thickness of bronchial wall (Wat/Pbm), smooth muscel layer(Wam/Pbm) and collagen fibers(Wac/Pbm) in model group [(155.32±39.92),(22.11±3.42),(8.47±3.01) μm2/μm] were all significantly increased than those in normal group [(71.01±11.02),(5.51±1.76),(2.37±1.03) μm2/μm](P<0.01). Compared with model group, number of total leukocytes and the percentage of eosinophil,neutrophil,lymphocyte in BALF were significantly lower in Scropio-Scolopendra group [(100.67±15.04),(17.08±4.92),(4.07±1.08) μm2/μm](P<0.01).The thickness of bronchial wall (Wat/Pbm), smooth muscel layer(Wam/Pbm) and collagen fibers(Wac/Pbm) were significantly decreased in Scropio-Scolopendra group (P<0.01). Conclusion: Scropio-Scolopendra can reduce airway inflammation of asthmatic rats, and play an inhibitory role in airway remodeling by reducing the bronchial wall and smooth muscle thickness, the proliferation of collagen fibers.关键词：Scropio;Scolopendra;asthma;airway inflammation;airway remodeling
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the effects of Huangqin Tang on the IL-23/IL-17 pathway in 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis model. Method: SD rats were distributed into four group, containing TNBS-induced colitis,Huangqin Tang(1.2 g·kg-1) or mesalazine treatment group(10 mL·kg-1,ig),negative control with no treatment and the last one was normal sham rats. Except for control group,experimental colitis was induced by rectal instillation of 2,4,6-trinitrobenzene sulfonic acid (TNBS) into rats. Drugs were given intragastrically for one week in each group continuously according to respectively-designed doses. The protein expressions of IL-17 and IL-23 in serum were determined by ELISA. The mRNA expressions of IL-17, IL-23, IL-17R, IL-23R and RORγt were determined by Real-time PCR. Result: Huangqin Tang markedly attenuated TNBS-induced colitis and effectively inhibited the protein expressions of IL-17(262.75±17.19) ng·L-1 and IL-23(56.75±6.20) ng·L-1. Furthermore, mRNA levels of IL-17(2.606±0.8),IL-17R(5.33±1.10),IL-23(2.16±0.19),IL-23R(3.34±0.70),RORγt(0.74±0.17)showed a marked reduction after Huangqin Tang teatment. Conclusion: Taken together, our findings demonstrate that Huangqin Tang can exerte therapeutic effects on TNBS-induced colitis, mediate probably by the IL-23/IL-17 pathway.关键词：IL-23/IL-17 pathway;colitis;Huangqin Tang
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发布时间：2024-01-04 - 摘要：Objective:To study the active fractions in liver protection from Erzhi Pill(AFEP) on acute hepatic injury induced by carbon tetrachloride(CCl4)in mice. Method: Sixty Kunming mice were randomly divided into six groups:the normal group,the model group,bifendate group (150 mg·kg-1),high AFEP group (19.8 g·kg-1),middle AFEP group (13.2 g·kg-1)and low AFEP group (6.6 g·kg-1).The treatment groups were orally administered once per day for 7 d separately,whereas the normal and model groups were orally administered with saline. Except normal rats,all the other rats were injected intraperitoneally CCl4 20 mL·kg-1 once. The rats were sacrificed 16 h after CCl4 administration. Serum and liver samples were collected for analysis. The acute hepatic injury model was prepared by CCl4 injected intraperitoneally. Then,the therapeutic effects of AFEP on the model were evaluated by the activity determination of serum alanine aminotransferase(ALT) and aspirate aminotransferase (AST),superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver,and the hepatic pathohistological changes following the treatment. Result: The activities of ALT and AST and the MDA content in liver was significantly increased and the activity of SOD was largely inhibited in the animals of modeling group. Following the treatment with AFEP,ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group.Furthermore,AFEP could ameliorate the hepatic pathological changes. Conclusion: AFEP have protective effects on acute hepatic injury induced by CCl4 in mice,and are the effect of the liver protecting active sites.关键词：Erzhi Pill;liver protection;active fractions;carbon tetrachloride;acute hepatic injury
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发布时间：2024-01-04 - 摘要：Objective:To observe the effects of Yulangsan polysaccharides (YLSPA) on behavior, structure changes of hippocampus in mice with chronic stress-induced depression. Method: Fifty Kunming mice were randomly divided into 5 groups: normal control, animal model, fluoxetine 20 mg·kg-1 and YLSPA 1 200 mg·kg-1, YLSPA 600 mg·kg-1 groups with continuous oral administration for 21 d, and chronic unpredictable mild stress (CUMS) with isolated support was imposed simultaneously to establish mouse depression model. The behavior changes of mice were evaluated by sucrose water consumption and the spontaneous activity; the structure changes of hippocampus were detected by the brain tissue HE staining. Result: The weight, the number of active stand, the consumption and preference for sucrose solution of the animal model group were significantly decreased compared with normal control group (P<0.01 or P<0.05), and were increased in YLSPA group and fluoxetine group (P<0.01 or P<0.05). Chronic unpredictable mild stress with isolated support could damage the structure of hippocampus, reduce the number of cells, YLSPA and fluoxetine could ameliorate these changes. Conclusion: YLSPA can improve the behavior scores of the mice with depression and ameliorate the damage of hippocamus induced by chronic unpredicted mild stress.关键词：YLSPA;chronic stress-induced depression;behavior;hippocampus
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发布时间：2024-01-04 - 摘要：Objective:To study the anti-inflammatory and antibacterial effects of Gegen Qinlian decoction prepared by different technology. Method: The swelling of ear induced by xylene in mice was employed to evaluate the anti-inflammatory effects of Gegen Qinlian decoction prepared by different technology. Additionally, the model of bacteriostasis in vitro by the serum with drug was used to examine the antibacterial activities of those. Result: Compared with mixed decoction and separated decoction, extract of active ingredients (1.95 g·kg-1) strongly inhibited swelling of ear in mice(P<0.01). The serum with extract of active ingredients significantly inhibited 10 kinds of strains such as Escherichia coli, Staphylococcus aureus and Salmonella typhi, etc. (P<0.01). Conclusion: Extract of active ingredients can exert significant inhibitory action against inflammation caused by chemical agents, and the serum with extract of active ingredients also has strong antibacterial activity.关键词：Gegen Qinlian decoction;preparation process;anti-infection;serum pharmacology
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发布时间：2024-01-04 - 摘要：Objective:To investigate the intervention effect of Shenqi Wan on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. Method: DMN (10 mg·kg-1) was administrated intraperitoneally to DMN-treated rats for the first three days per week for 4 weeks, at the second weekend, DMN rats were randomly divided into Shenqi Wan group(n=10)and modle group(n=10). At the first day of the third week, both groups continued to receive weekly DMN treatment for another 2 weeks in addition to daily administration of Shenqi Wan. At the end of the experiment, all rats were sacrificed. Liver tissue specimens and serum were remained to observe liver function, liver tissue hydroxyproline (Hyp) and liver histopathological changes of rats, expression changes of liver tissue hepatocyte growth factor (HGF), smooth muscle actin (alpha-SMA) mRNA were observed by real-time quantitative PCR. Result: Liver dysfunction was based on DMN modeling for four weeks, compared with normal group, the serum aspartate aminotransferase (ALT) and alanine aminotransferase (AST) activity and total bile acid(TBA) content in the fourth week model group were significantly increased (P<0.01), serum albumin (ALB) were significantly decreased (P<0.01), liver hydroxyproline content was significantly increased 4.58 fold (P<0.01), HGF mRNA expression was decreased significantly (P<0.01), α-SMA mRNA levels were significantly increased (P<0.01). Compared with model group, serum ALB content in Shenqi Wan group was significantly increased, the serum aspartate aminotransferase (ALT) and alanine aminotransferase (AST) activity and TBA content were significantly decreased. Shenqi Wan could significantly reduce liver tissue hydroxyproline content (P<0.05). Shenqi Wan could significantly reduce α-SMA mRNA expression (P<0.01), and elevated HGF mRNA level (P<0.05). Conclusion: Shenqi Wan has a good effect for liver fibrosis and provides an experimental basis for liver disease treatment from kidney.关键词：Shenqi Wan;DMN;liver fibrosis
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发布时间：2024-01-04 - 摘要：Objective:To study the antioxidant activity of different polarity chemical constituents from Tetrastigma planicaule. Method: The capacity of ferric reducing antioxidant power (FRAP) and scavenging hydroxyl free radical (OH·) and·) of the total extracts, petroleum ether fraction, ethylacetate fraction, n-butanol fraction and water fraction from T. planicaule were evaluated by FRAP assay, OH·scavenging assay, and ABTS+·scavenging assay at different concentration (0.005-0.050 g·L-1). Results were compared with that of positive control vitamin C(VC) and tea polyphenol. Result: First, ferric reducing antioxidant power of different polarity chemical constituents from T. planicaule were weaker than VC, but higher than tea polyphenol except water fraction. When the concentration of different polarity chemical constituents was 0.050 g·L-1, ferric reducing antioxidant power was as follows: VC (FRAP value 2 377.8)>total extracts (FRAP value 683.8)>ethyl acetate (FRAP value 419.8)>petroleum ether (FRAP value 201.8)>n-butanol (FRAP value 185.8)>tea polyphenol (FRAP value 73.8)>water (FRAP value 63.8). Secondly, the results demonstrated that OH·scavenging activity in ethylacetate fraction (IC50 0.028 g·L-1) was higher than VC (IC50 0.044 g·L-1) and tea polyphenol (IC50 0.032 g·L-1). Finaly, different polarity chemical constituents showed higher ABTS+·scavenging activity than tea polyphenol (IC50 0.416 g·L-1), also ethylacetate fraction (IC50 0.008 g·L-1) was higher than VC (IC50 0.011 g·L-1). Conclusion: T. planicaule has a strong antioxidative activity, which provides scientific basis for exploiting functional antioxidant.关键词：Tetrastigma planicaule;antioxidant activity;FRAP assay;OH·;ABTS assay
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of Lanyin Fengyang Huazhuo Jiedu prescription on expressions of CD4+/CD29+,IL-8 and IL-10 in rats with ulcerative colitis. Method: Sixty healthy male Wistar rats were randomly divided into 5 groups: the normal group(NG), model control group (MG), high-dose group of Lanyin Fengyang Huazhuo Jiedu decoction (HDG,20.0 g·kg-1), low-dose group of Lanyin Fengyang Huazhuo Jiedu decoction (LDG,5.0 g·kg-1) and the sulfasalazine group(SPAP 0.3 g·kg-1),12 rats in every group. Trinitrobenzenesu l-fonicacid/ethanol(TNBs/ethanol) was used to induce ulceratvie colitis. After successful modeling, continuous treatment lasted 14 days. The rat's general state, disease activity index(DAI) scores,colonic pathological morphology change of T-lymphocyte cytokines IL-8 and IL-10 were observed. Result: DAI scores: compared with NG,the scores of other groups were elevated. compared with the MG, the scores of HDG and LDG were reduced. The result showed statistical significance (P<0.05). CD4+,CD29+: compared with the MG, the levels of NG,HDG and LDG were reduced. The result showed statistical significance (P<0.05). IL-8: compared with the MG, the levels of NG,HDG and LDG were reduced (P<0.05). There was no difference between the levels of LDG and SG. IL-10: compared with the MG, the levels of SPAP,HDG and LDG were elevated(P<0.05). Conclusion: Lanyin Fengyang Huazhuo Jiedu decoction can raise the suppression of inflammatory cytokines,reduce promoting the inflammatory factor and suppressioned of CD4+/CD29+expression关键词：ulceratvie colitis;Lanyin Fengyang Huazhuo Jiedu prescription;IL-8;IL-10;T cell subsets
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发布时间：2024-01-04 - 摘要：Objective:To observe the effects of 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glycoside (TSG) on expression of β-amyloid precursor protein (APP) mRNA and presenilin-1(PS1) mRNA in senescence accelerated mouse (SAMP8). Method: Fifty six-month-old SAMP8 mice were randomized into 5 groups: SAMP8 untreated control group, huperzine A control group, low-, mid-and high-dose groups of TSG(0.033, 0.1, 0.3 g·kg-1·d-1), with 10 mice in each group. Another ten 6-month-old SAMR1 mice were assigned to normal control group. After medication for 60 days, expression of APP and presenilin-1 (PS1) mRNA in hippocampus was assayed by real-time quantitative reverse transcription-PCR. Result: The expression of APP and PS1 mRNA in all dose of TSG groups was down-regulated. Compared with SAMP8 untreated control group, there was significant differences (P<0.05). Conclusion: TSG can inhibit the expression of APP mRNA in SAMP8 hippocampus, so as to play the role of anti-senile dementia.关键词：2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glycoside;Alzheimer's disease;APP;PS1;real-time quantitative reverse transcription-PCR
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发布时间：2024-01-04 - 摘要：Objective:To study the protective effect of the Gynostemma pentaphyllum polysaccharide on the CCl4-induced liver injure in rats. Method: The liver injure was induced by carbon tetrachloride(CCl4) in rats, rats were divided into four groups randomly: model group, Bifendate group (40 mg·kg-1), low-and high-dose groups of G. pentaphyllum polysaccharide (40, 80 g·kg-1), as well as normal group was set up for 30 consecutive days. At the end of 30 days, the blood was collected and the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were examined, the expression of inducible nitric oxide synthase(iNOS) mRNA in liver were examined by reverse transcription polymerase chain reaction(RT-PCR) analysis. The expression of B cell lymphoma/leukmia-2(Bcl-2)/Bcl-2 associated X protein(Bax) in hepatic tissue was determined by Western blot analysis, and the changes of hepatic histopathology was observed by HE-staining. Result: Compared with model control group, G. pentaphyllum polysaccharide significantly decreased the levels of AST and ALT in liver injure induced by CCl4in rats (P<0.05), while the iNOS mRNA expression in hepatic tissue was down-regulated (P<0.05). The level of Bcl-2/Bax was elevated in hepatic tissue (P<0.05), and liver injure was alleviated. Conclusion: The results disclose that G. pentaphyllum polysaccharide has protective effect on the CCl4-induced liver injure in rats, mechanism of which may be related to inhibiting the cytotoxicity and anti-apoptotic pathways.关键词：Gynostemma pentaphyllum polysaccharide;liver injure;iNOS;anti-apoptotic effects
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发布时间：2024-01-04 - 摘要：Objective:To observe the influence of energy charge and the expression of ciliary neurotrophic factor mRNA(CNTF mRNA) by Fuzi Lizhong decoction in rats with spleen yang deficiency syndrome. Method: Animals were divided into the control goup,the model group and the Fuzi Lizhong decoction group. The model group was insised brown adipose tissue then fed with high fat diet under 19℃ circumstance,which lasted for 3 weeks.The Fuzi Lizhong decoction group was ig 5 mL·kg-1 Fuzi Lizhong decoction based on the model group once a day, for 4 weeks. Testing energy charge of skeletal muscle and the expression of CNTF mRNA were assayed by the method of reverse transcription-polymerase chain reaction(RT-PCR). Result: Compared with the control group(0.042±0.018), energy charge decreased in the model group (0.032±0.014)and the Fuzi Lizhong decoction group (0.024±0.012 )( P<0.05 or P<0.01). Compared with the model group, energy charge decreased significantly (P<0.05). Compared with the control group(0.862±0.072),the expression of CNTF mRNA in the model group (0.426±0.056) decreased(P<0.01). Compared with the model group, the expression of CNTF mRNA in the Fuzi Lizhong decoction group (0.838±0.068)increased (P<0.01). Conclusion: It is suggested that Fuzi Lizhong decoction can regulate the expression of CNTF mRNA, which indicated an effect on nutrition and energy metabolism of skeletal muscle by up-regulating the expression of CNTF mRNA and increasing the content of CNTF.关键词：Fuzi Lizhong decoction;spleen yang deficiency syndrome;energy charge;ciliary neurotrophic factor
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of dencichine injection on nervous system. Method: The rats were given drug by iv once daily for 7 consecutive days. The SD rats were divided into 4 groups randomly: normal saline(NS)group, the low dose group, middle dose group and high dose group, the effect of dencichine for injection on autonomic activities was observed after administration of 1, 2 h,7 d and withdrawal of injection. The SD rats were divided into 5 groups randomly: normal saline (NS) group, the low dose group, middle dose group, high dose group and positive control group(7.5 mg·kg-1 Pentobarbital sodium), the effect of dencichine for injection on concordant ability was observed after administration of 1 h, 7 d and withdrawal of injection. Result: Dencichine injection at the dose of 135 mg·kg-1 reduced autonomic and concordant ability of rats,this phenomenon disappeared after stopping injecting drugs. Dencichine injection at the dose of 45 mg·kg-1 increased autonomic ability of rats, but the phenomenon disappeared soon, and the dose of 45 mg·kg-1 had no effect on the concordant ability. Dencichine for injection at the dose of 15 mg·kg-1 increase autonomic ability of rats in a short time after stopping injecting drugs,but it had no effect on the concordant ability. Conclusion: Dencichine injection at the dose of 135 mg·kg-1 has obvious effect on nervous system. Dencichine injection at the dose of 45 mg·kg-1 has excitatory effects on the nervous system in a short period of time. Dencichine for injection at the dose of 15 mg·kg-1 have no effect on the nervous system, which can be believed a safety dose.关键词：dencichine for injection;safety pharmacology research;nervous system;autonomic ability;concordant ability
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发布时间：2024-01-04 - 摘要：Objective:To investigate the protective effects of Xinjining (XJN) on acute myocardial ischemia. Method: Acute myocardial ischemia model in rats was established by injecting pituitrin(Pit) into sublingua vein. SD rats were randomly divided into 6 groups: control group, model group, Wenxin granule group (9.0 g·kg-1), XJN groups (6.0, 3.0, 1.5 g·kg-1). We monitored electrocardiography(ECG) and recorded the cardiographs of all the groups at the times of Pit pro-injection and post-injection, moreover, compared the change ratio of the T wave height and heart rate (HR), detected the contents of malonaldehyde (MDA), superoxide dismutase (SOD), phosphocreatine kinase (CK) and lactate dehydrogenase (LDH) of blood serum. The acute myocardial ischemia model in SD rats was established by ligating the left anterior descending coronary artery (LAD). The rats were randomly divided into control group, model group, Wenxin granule group (9.0 g·kg-1) and XJN group (3.0 g·kg-1). After 4-day intervention, the acute myocardial ischemia model was established by LAD. After the model established successfully, the rats were given another 3-day intervention, we recorded the hemodynamic indexes, such as, arterial pressure systolic blood pressure(SBP),diastolic blood presure(DBP), heart rate(HR), the left ventricular systolic pressure (LVSP), the left ventricular end diastolic pressure (LVEDP) and the maximum pressure variation rate of left ventricular in isovolumic phase (±dp/dtmax). Meanwhile,we calculated the data of the left ventricular developed pressure (LVDP). Moreover the activity of SOD, the contents of MDA, CK and LDH in blood serum were determined, and the myocardial infarct size (MIS) was measured after tripheryltetrazolium chloride (TTC) dyeing. Result: XJN (6.0,3.0 g·kg-1) could obviously reduce the changes of T wave at the times of 15 s, 2,5 min (P<0.01 or P<0.05).XJN (6.0, 3.0, 1.5 g·kg-1) could ameliorate HR at the times of 15 s (P<0.05), 30 s (P<0.01).XNJ (6.0, 3.0 g·kg-1) could markedly increase the activity of SOD, decrease the contents of LDH, CK, MDA in rats induced by Pit (P<0.01 or P<0.05). XJN (3.0 g·kg-1) could increase the activity of SOD without significance, increase LVSP, LVDP and+dp/dtmax significantly, decrease the contents of MDA, LDH, CK, LVEDP and MIS significantly (P<0.01 or P<0.05) in rats induced by LAD. Conclusion: XJN has significantly protective effects on acute myocardial ischemia in rats.关键词：Xinjining;myocadial ischemia;Pituitrin;coronary artery ligation;ECG;biochemical indexes of blood serum;hemodynamics
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发布时间：2024-01-04 - 摘要：Objective:To study the protective effect of the Niuxin persimmon leaf polysaccharide on kidney injure in diabetic mice induced by streptozotocin(STZ) and its mechanisms. Method: Being established of diabetic mice model, the mice were randomly assigned to 5 groups: model group, melbinum group (0.32 g·kg-1), low, medium and high-dose groups of Niuxin persimmon leaf polysaccharide (0.3, 0.6, 1.2 g·kg-1) for 15 consecutive days. At the end of 15 days, oxidase method was used to examine the blood glucose levels (fasting blood-glucose,FBG), while the endpoint method was used to measured urinary albumin content (24 h). The changes in renal morphology were tested through HE-staining. The expressions of insulin-like growth factors-1(IGF-1) in renal tissue were determined using Western blot analysis. Result: Compared to model control group, Niuxin persimmon leaf polysaccharide could notably diminish the FBG in diabetic mice (P<0.05), and measured urinary albumin content was reduced statistically (P<0.05). The IGF-1 protein level in renal tissue was up-regulated (P<0.05), and the condition of renal injury alleviated finally. Conclusion: The results indicate that Niuxin persimmon leaf polysaccharide has protective effect on renal injury in diabetic mice induced by STZ. The mechanism may be related to inhibition of IGF-1 expression in renal tissue to regulate its downstream pathway.
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发布时间：2024-01-04 - 摘要：Objective:To study the protect effects of Ganbao capsule on liver-injury induced by carbon tetrachlotide(CCl4)ethanol and acetaminophen in mice. Method: Sixty KM mice were divided into 6 groups randomly: blank control group, model group with liver injury, three experimental groups (Ganbao capsule 0.125, 0.25, 0.5 g·kg-1 respectively), positive control group (biphenyl double ester 0.2 g·kg-1). CCl4, ethanol and acetaminophen were accordingly given to mice by oral gavage once in order to make the acute liver injury model at the end of experiment. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) were measured, the liver index was simultaneously determined. In addition in CCl4-induced model, hemolysin, spleen and thymus indexes were measured. Result: Ganbao capsule high, middle dose could obviously reduce ALT, AST, liver index for the liver injury models(P<0.05 or P<0.01). High dose could reduce AKP's content in the mice model induced by acetaminophen(P<0.05), and significantly increase hemolysin content in the CCl4 model, and reduce the thymus index and spleen index in the same model(P<0.05 or P<0.01). Conclusion: Ganbao capsule has the function of protecting the liver from liver injury induced by CCl4, ethanol and acetaminophen.关键词：Ganbao capsule;liver injury;CCl4;acetaminophen
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发布时间：2024-01-04 - 摘要：Objective:To study the effects of epigallocatechin-3-gallate(EGCG)on transferrin(Tf)in liver of mice with alcoholic liver disease(ALD) and to explore its mechanisms. Method: C57/BL6 mice were randomly divided into two groups: normal group and model group. Alcoholic liver disease was induced by gavage of alcohol for 12 weeks. At the end of 8 weeks, the alcohol group was divided into four subgroups: model group, EGCG (20 mg·kg-1) group, EGCG (10 mg·kg-1) group, EGCG (30 mg·kg-1) group. Mice in the EGCG groups were received an intraperitoneal injection of EGCG via simultaneous intragastric administration with normal saline or alcohol for 4 weeks. Liver injuries were assessed by histopathologic examination and serum alanine aminotransferase(ALT), aspartate aminotransferase(AST)levels. In addition, liver iron levels and Tf, transferin receptor 1(TfR1)of liver tissue were evaluated. Result: Model group mice had marked increase in serum ALT, AST levels and liver iron concentration compared with normal group, and their liver tissues showed moderate hepatocytes fatty degeneration. However, every treatments groups resulted in decreased ALT, AST levels and liver iron concentration and improved pathological changes. Liver Tf and TfR1 protein expression levels were elevated significantly in model group compared with normal group, but markedly suppressed by EGCG treatments. Conclusion: Compared with model group, treatment of ALD mice with EGCG decreased Tf and TfR1 protein expression in the liver. EGCG might play a protective role in the development of ALD. This beneficial effect of EGCG may be attributed to its iron chelation ability. The possible mechanisms is that EGCG decreases the hepatic iron uptake by the downregulation of Tf and TfR1.关键词：epigallocatechin-3-gallate;alcoholic liver disease;iron overload;transferrin;transferin receptor 1
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发布时间：2024-01-04 - 摘要：Objective:By measuring the level of myeloperoxidase (MPO), infarction weight ratio, myocardial cell morphology, to observe the myocardial protective effects and possible mechanism of Tiaozhi Tongluo capsules for myocardial ischemia-reperfusion injury (MIRI). Method: Ninety-six male Wistar rats were fed with high fat diet for 4 weeks except the non-fat model group. The rats were randomized into six groups and drug intervention or saline for 4 weeks: (1) fat model group; (2) non-fat model group; (3) statin group; (4) Tiaozhi Tongluo capsules 8.64 g·kg-1·d-1; (5) Tiaozhi Tongluo capsules 4.32 g·kg-1·d-1; (6) Tiaozhi Tongluo capsules 2.16 g·kg-1·d-1. The same volume of saline was infused in fat model and non-fat model group. After 2 hours of the last intragastric infusion, the chest was opened and the left anterior desending coronary arteries (LAD) was ligated for 30 min and unclamped for 24 hours for all the rats. After 24 h of reperfusion, the level of blood lipids, cardiac enzymes in serum or the content of MPO in ischemic myocardium tissue (half of the animals of each group) were measured. Myocardial tissue near the apical about 0.5 cm2 was taken for HE staining, morphological changes were observed. The myocardial tissue of the rest animals in each group was stained by Evans blue and TTC. Then these slices were immersed in 4% paraformaldehyde formalin, ischemic zone size (IZS), infarct size (IS), the ischemic weight ratio and the infarction weight ratio were determined. Result: The level of cardial enzymes, MPO and infarction weight ratio were significantly higher in fat model group compared to the non-fat model group(P<0.01). The drug treatment groups could significantly reduce the level of cardial enzymes, MPO and infarction weight ratio compared to fat model group(P<0.01). Conclusion: Tiaozhi Tongluo capsules and atorvastatin could protect myocardial cells by reducing the infiltration of inflammatory cells, which might be related to the effects of lowering blood lipids and protecting endothelial function. No clear does-dependent effects were found for Tiaozhi Tongluo capsules.关键词：Tiaozhi Tongluo capsules;myocardial ischemia-reperfusion injury;myeloperoxidase;blood lipid
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发布时间：2024-01-04 - 摘要：Objective:To observe the effects of Furong Tongmai capsule (FTC) on the plasma superoxide dismutase(SOD),malondialdehyde (MDA)levels and the expression of nerve growth factor(NGF) in sciatic nerve in diabetic rats, and to explore possible mechanism on diabetic neuropathy. Method: Wistar rats were randomly divided into normal group and diabetic groups. The diabetic rats were randomly divided into model group,FTC groups (0.7,1.4, 2.2 g·kg-1 accordingly). After treated 8 weeks, levels of plasma glucose,SOD, MDA were observed. The NGF of sciatic nerve was detected by immuneoshistochemistry array. Result: The levels of blood glucoses in diabetic rats were significantly higher than that in control group. Compared with model group, FTC 1.4, 2.2 g·kg-1 groups showed increase in SOD were significantly. Higher level of SOD was found in FTC 1.4, 2.2 g·kg-1 groups than those in the model group, while the levels of MDA were lower in the FTC 1.4, 2.2 g·kg-1 groups than in the model group. By analysing the NGF of sciatic nerve, the positive rate of NGF expression in sciatic nerve was significantly lower in model and FTC 0.7 g·kg-1 group than that in the control group. The positive rate of NGF expression was significantly higher in the FTC 1.4, 2.2 g·kg-1 groups than those in the model and FTC 0.7 g·kg-1 group. Conclusion: FTC may antagonise oxidative stress by increasing plasma SOD level and reducing plasma MDA level. FTC could increase NGF expression in sciatic nerve in diabetic rats.关键词：Furong Tongmai capsule;diabetic neuropathy;oxidative stress;nerve growth factor
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发布时间：2024-01-04 - 摘要：Objective:To study the effect and possible mechanism of Poge Jiuxin decoction(PGJXD) on heart failure(HF) after infarction induced by Yang weak and body fluid flood syndrome in rats. Method: Rat model of HF was induced by adriamycin. The model-established rats were randomized into model group,PGJXD high-dosage group(18.66 g·kg-1),low-dosage group(9.33 g·kg-1) and the fosinopril sodium group(4.67 mg·kg-1). A blank control group was also set up. Drugs were given by continuous intragastric administration for 28 days. Then cardiac function of each group was evaluated by echocardiography,plasma aldosterone(ALD) and angiotensin Ⅱ(AngⅡ) levels were also examined by radioimmunoassay(RIA) in all groups,and myocardial pathology biopsy was carried out. Result: Compared with the blank group,the left ventricular end-diastolic diameter(LVEDd) and left ventricular end-systolic diameter(LVEDs) of model group increased significantly(P<0.05),ejection fraction(EF)decreased significantly(P<0.05). After given the high-dose and middle-dose of PGJXD and the fosinopril sodium, EF and cardiac function were improved. Moreover,PGJXD could effectively shorten the LVEDd and LVEDs,and fosinopril sodium did not work. RIA results showed that ALD and AngⅡ in plasma of model group was significantly higher than those in blank group(P<0.05);the high-dose and middle-dose of PGJXD and the fosinopril sodium could down-regulate the plasma ALD and AngⅡ(P<0.05). In the models, myocardial pathology investigation suggested myocardial fibers derangement,with visible dyeing depth,fracture and zoning,part of the muscle fibers were separated from each other. Compared with the model group,the high-dose and middle-dose of PGJXD and the fosinopril sodium showed obvious reduced myocardial fiber breakage. Conclusion: PGJXD can shorten the LVEDd and LVEDs,and thus reverse myocardial remodeling and strength the cardiac function. The mechanism may be related to decrease in plasma ALD and AngⅡ and reduce in renin-angiotensinrenin-angiotensinaldosterone system(RAAS) activity thus to improve cardiac afterload in HF Rats.关键词：Poge Jiuxin decoction;heart failure;cardiac function;aldosterone;angiotensin Ⅱ
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of Gegen Qinlian decoction on myocardial ischemia reperfusion induced arrhythmia in rats, to provide experimental evidence for clinical application. Method: Healthy SD rats were randomly divided into six groups (n=10, each), sham operation group (given equal volume distilled water), model group (given equal volume distilled water), Wenxin keli group (2.43 g·kg-1), Gegenqinlian decoction high, medium, low dose (8.64, 4.32, 2.16 g crude drug·kg-1) groups. Beside the sham operation group, the other groups were performed by liquating anterior interventricular branch of the left desending coronary artery to establish myocardial ischemia reperfusion induced arrhythmia. After different intervention for continuous 7 days, electrocardiogram of lead II was examined, and tissue Na+-K+-ATP enzyme (Na+-K+-ATPase), Ca2+-ATP enzyme (Ca2+-ATPase) and serum superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. Result: Gegenqinlian decoction could significantly reduce the incidence of arrhythmia, serum MDA and increase serum SOD, also significantly increase Na+-K+-ATPase, Ca2+-ATPase activity (P<0.01 or P<0.05). Conclusion: Gegenqinlian decoction has good anti-arrhythmia effect in the way of increasing enzymes activity and removing oxygen free radicals, to improve arrhythmia and protect the ischemic myocardium.关键词：Gegenqinlian decoction;anti-arrythmia;myocardial ischemia/reperfusion;serum superoxide dismutase;malondialdehyde;Na+-K+-ATPase;Ca2+-ATP ase
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发布时间：2024-01-04 - 摘要：Objective:To study the expectorant,analgesic and antibacterial effects of Yanshuning granules. Method: Mice were divided into 6 groups randomly: normal control group, positive groups, high dose group, middle dose group and low dose group of Yanshuning granules (11.00,5.50,2.75 g·kg-1), after 7 d by ig administration,the expectorant effect was studied by a mouse model of phenol red test. The analgesic effect was studied by mouse models of hot-plate induced pain and acetic acid-induced writhing reaction.Experimental research on antibacterial action in vitro of Yanshuning granules was applied to observe the antibacterial effect. Result: The experimental results showed that Yanshuning granules could significantly increase phenol red excretion in mice (P<0.05 or P<0.01). It could significantly prolong the latent time of reaction on hot-plate in mice(P<0.05), reduce the writhing times in mice(P<0.05 or P<0.01).The granules showed obvious antibacterial actions against Staphylococcus aureus[minimal inhibitory loncentration(MIC) is 0.250 g·mL-1, minimal bactericidal concentration(MBC) is 0.500 g·mL-1], A-hemolytic streptococcus(MIC is 0.062 g·mL-1,MBC is 0.250 g·mL-1),Beta hemolytic streptococcus(MIC is 0.031 g·mL-1,MBC is 0.062 g·mL-1),Proteus(MIC is 0.125 g·mL-1,MBC is 0.250 g·mL-1) and Streptococcus pneumoniae(MIC is 0.125 g·mL-1,MBC is 0.500 g·mL-1). Conclusion: The granules has obvious expectorant,analgesic and antibacterial effects.关键词：Yanshuning granules;pharmacodynamics;expectorant effect;analgesic effect;antibacterial effect
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发布时间：2024-01-04 - 摘要：Objective:To discuss the effects and active mechanism of gallic acid (GA) as main active compound in Ampelopsis japonica (AJ) to HepG2 cells proliferation. Method: The MTT method was used to determine restriction activity of GA to HepG2 cells proliferation after HepG2 cells were treated with different GA concentration. Hoechst dyeing method, fluorescence microscope, annexin V-FITC/PI double-labeling method and flow cytometry method were used to observe morphological structure change of apoptotic cells and to detect cell apoptosis quantitatively and qualitatively. Finally, JC-1 dyeing method was used to detect the potential change of HepG2 cells mitochondrial membrane. Result: The restriction of GA to HepG2 proliferation is effective in concentration of 12.5-200 mg·L-1. GA is concentration-dependence, and has an ability of inducing HepG2 cells apoptosis and reducing potential of mitochondria. Conclusion: GA is one of principle anti-tumor constitutes of AJ. And one of its anti-tumor mechanism is inducing cell apoptosis by reducing potential change of mitochondria.关键词：gallic acid(GA);HepG2 cells;cell apoptosis;potential of mitochondrial membrane
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of Sini decoction(it consist of Radix Aconiti Praeparata, Rhizoma Zingiberis and Radix Glycyrrhizae Preparata) on high density lipoprotein cholesteol(HDL-CH),low density hpoprotein cholested( LDL-CH) and apolipoprotein (Apo)-A, B on the hyperlipidemia (HLP) combined with atherosclerosis in rabbits. Method: The rabbits were fed with high fat food everyday and injected with BSA, egg-white protein to make HLP combined AS modle, then randomized them into model group and three dose groups treated by Sini decoction(high, medium and low) and Atorvastatin respectively. Colorimetric assay method was used to determine LDL-CH, HDL-CH levels and electrophoresis wa used to measure levels of apolipoprotein A and B changes. Result: Sini decoction high-dose group, decreased LDL and Apo-B level compared with the model group (P<0.05). Sini decoction hihg dose group incresed HDL,Apo-A content, HDL/LDL and Apo-A/Apo-B ratio compared with the model group (P<0.01). Conclusion: Sini decoction can increase HDL and Apo-A content, reduce the content of LDL and Apo-B, which may be one of the mechanisms of the anti-hyperlipidemia with atherosclerosis rationale by Sini decoction.关键词：Sini Decoction;hyperlipidemia;artherosclerosis;HDL-CH;LDL-CH;Apo-A, Apo-B
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发布时间：2024-01-04 - 摘要：Objective: To explore the impact of Xinjikang program on syndrome of the acute phase of viral myocarditis. Method: One hundred and sixty-one cases from Henan Chinese medical hospital, Heilongjiang Academy of traditional Chinese medicine(TCM), Shanghai University of TCM, Longhua Hospital three hospital, entered the study. Cases were randomly divided into treatment group and control group. The treatment group was treated by Xinjikang program plus basic treatment, control group was treated by conventional western medicine plus the basic measures. The course lasted for 30 days. The patient's syndromes were observed. Result: The difference of syndrome total score between the two groups was statistically significant (P<0.05). The difference of total score syndrome at various time point between the two groupswas statistically significant (P<0.05). The difference of total score syndrome before and after treatment was statistically significant (P<0.05). Conclusion: Xinjikang program and routine western medicine can improve the syndromes in patients with syndrome of the acute phase of viral myocarditis but efficiency of Xinjikang program was significantly superior to conventional western treatment.关键词：Xinjikang, programs;viral myocarditis;traditional Chinese medicine syndromes
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发布时间：2024-01-04 -
Comparison of Efficacy in Treatment of Premature Beat between Buchang Wenxin Granule and Propafenone
摘要：Objective: To compare the efficacy and clinical features of Buchang Wenxin granule and propafenone in the treatment of premature beat. Method: One hundred and seven patients with premature beats (supraventricular SV, n=52;ventricular V, n=55)randomly were divided into Wenxin granule group(W group, n=62)and propafenone group(P group, n=45),the patients in W group were given Buchang Wenxin Granule 9 g·d-1, 3/d, the patients inP group were given propafenone 150 mg·d-1, 3/d. 4 weeks later, all patients were evaluated by 12-lead simultaneous body surface electrocardiogram and 24 hours ambulatory Holter, respectively compares the percentage of reduced premature beat, 24 h average heart rate, the slowest heart rate, QTcd before and after treatment. Result: Wenxin Granule in therapeutic SV,V are respectively 73.53% and 78.57%, the total efficiency is 75.81%, have no significantly difference compared with propafenone; the 24 h average heart rate and the slowest heart rate of W group had not decreased significantly before and after treatment(77.67±5.86) bpm vs (77.42±4.99) bpm, (60.89±5.40) bpm vs (60.55±4.71) bpm; while P group had decreased significantly before and after treatment(78.51±5.42) bpm vs (76.96±5.57) bpm (P<0.05),(61.31±5.69) bpm vs (58.53±4.80) bpm (P<0.05). Wenxin rranule also can reduce the QTcd of patients with Diabetes mellitus(DM) and coronary heart disease(CHD). Conclusion: Buchang Wenxin granule is effective for treating premature beats, suitable for the patients with slower heart rate,DM,CHD.- 2
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发布时间：2024-01-04 - 摘要：Objective:To investigate the therapeutic efficacy and mechanism of Huanglian Jiedu decoction in treatment of perianal abscess of severe type. Method: Patients with perianal abscess of severe type received operation in hospital were divided into two groups randomly, treatment group (n=30) took Huanglian Jiedu decoction orally on the basis of routine therapy after operation, control group (n=26) only received routine therapy. The body temperature, red and swollen around cut were observed. The level of white blood cell (WBC), serum endotoxin(ET), tumour necrosis factor α (TNF-α) of two groups before treatment and on the third day after treatment were tested. Result: There were no significant differences in all data before treatment between two groups. The body temperature and the number of WBC in treatment group was lower than those in control group on the third day, but there was no statistical difference. The degree of red and swollen around cut, serum ET, TNF-α on the third day after treatment in treatment group were lower than those in control group (P<0.01). Conclusion: Huanglian Jiedu decoction is an effective method in treatment of perianal abscess of severe type, which plays a therapeutic action through reducing serum endotoxin and down regulating the level of TNF-α.关键词：Huanglian Jiedu Decoction;perianal abscess of severe type;endotoxin;tumour necrosis factor α
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发布时间：2024-01-04 - 摘要：Objective:To study effect of Guling gao(GLG) and its components like Guo Gao(GUG),Ling Gao(LG) on osteoarthritis and determine the efficacy of optimal compatibility. Method: Experiment was divided into 3 groups,GLG, GUG and LG group for treatment of osteoarthritis(OA)in 153 cases,51 cases in each group; according to the visual analog scale(VAS),Lysholm knee function scores (LKSS) and other criteria were used to compare efficacy. Result: The treatment group greatly improved the VAS and LKSS score,reduce joint swelling and activity index,GLG, compared with GUG and LG group, was more obvious(P<0.05). Conclusion: GLG, compared with GUG and LG,has notablely curative effect in treatment of knee osteoarthritis.关键词：Drynaria fortunei;Clematis chinensis;soft extract;osteoarthritis
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发布时间：2024-01-04 - 摘要：Objective:To evaluate 5-hydroxytryptamine(5-HT) available for the pathogenic factors of irritable bowel syndrome(IBS), we use Meta-analysis method to study the role of in the pathogenesis of IBS. Method: The articles about the relationship with 5-HT and diarrhea predominant irritable bowel syndrome(IBS-D) and constipation predominant irritable bowel syndrome (IBS-C) were collected in CNKI, Weipu, PubMed from 2000 to 2011. Result: A total of 108 Chinese literatures and 98 foreign literatures were searched, and 5 studies met the inclusion criteria were identified for meta-analysis. There were significant differences between IBS-D group and the control group (P<0.05), IBS-C group and the control group (P<0.05); there was no difference between IBS-D group and IBS-C group. Conclusion: 5-HT plays a role in the pathogenesis of IBS-D and IBS-C, the role of 5-HT in IBS-D and IBS-C may be the same.关键词：irritable bowel syndrome;5-hydroxytryptamine;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective:To discuss the firmula regularity of Chinese herbal compound in the treatment of hand-feet-mouth disease in children. Method: The data was collected from January, 2000 to December, 2010.More than 126 Chinese herbal formulas used in 20 cases and without adverse reaction, were searched through the CNKI medical database; and the database was set up using Excel 2003 software, the name of each herbal medicine in the compound was collected for a statistical analysis. The herbs which were frequently used were analyzed to obtain formula rules. Result: Of the total 126 Chinese herbal compound in the treatment of hand-feet-mouth disease in Children, for 156 kind of hebs the frequence of application reached 1 625, and the top 10 in application were honeysuckle, glycyrrhiza and weeping forsythia,etc. Conclusion: The relationship between the three factors(heat evil, pathogenic wind, and pathogenic dampness) and hand foot and mouth disease was verified through the study of the Chinese herbal compound in the treatment of hand-feet-mouth disease in Children.关键词：hand foot and mouth disease;Chinese herbal compound formula;association rules
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发布时间：2024-01-04 - 摘要：Saponins of panax notoginseng is a mainly effective ingredient in the plant sanqi, which contains different kinds of monomer saponins. Sanqi belongs to the ginseng family. With the general application of sanqi in clinical, its function that improves the ischemia microenvironment has drew the attentions of researchers in recent years. This article summarizes the notoginsenoside efficacy in regulating blood vessels, protecting the vascular endothelial cells, eliminating edema, inhibiting oxidative stress, inhibiting apoptosis, affecting blood rheology and regulating immune function, etc, provides bases and new ideas for the researches of notoginsenoside improves abnormal microenvironment under experiment.关键词：panax notoginseng;abnormalities microenvironment of experiment brain;improvement;mechanism
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发布时间：2024-01-04 - 摘要：By reviewing the of Chinese and foreign literatures in recent two decades,the relative researchs of dissipating phlegm and resolving masses treating tumors had been arranged and analysed.The status that dissipating phlegm and resolving masses treats caicinoma had been summarized in the theoretical study, clinical trial, an experimental study,universal researchs and outstanding results had been obtained,however,the clinical utilization owes the standard and is short of foresightedness, multi-centers,random, blind trails,meanwhile,there was limited in depth and efforts,therefore,the understanding about dissipating phlegm and resolving masses should be searched deeply.It is obvious that dissipating phlegm and resolving masses is an essencial way in treating tumor and has universal prospects.关键词：resolving phlegm and dissipating a mass;tumor;theory investigation;clinical research;experiment study
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发布时间：2024-01-04 - 摘要：Molecular Compatibility theory was put forward to provide theoretical guidance for modern Chinese medicine(molecular Chinese medicine).They were expounded to the background, scientific connotation, necessity,features of Molecular Compatibility theory and characteristics of modern Chinese medicine. Molecular compatibility theory had three features.①It reflected the concept of organic combination of Chinese and western medicine.②Systematic thinking combined with analysis reduction method organically.③The overall concept of TCM theory was adhered to;Characteristics of modern Chinese medicine by molecular compatibility theory was three.①They were clear of the chemical structure, molecular formula, molecular weight of all ingredients in prescription. ②The principle of dunchenzuoshi was followed. ③It had clear mechanism, definite effect, advanced dosage forms, stable quality control. ④ Multichannel, multi-evel and multi-channel network pharmacological effects were produced in sick body.⑤Efficacy was obvious and side effects were small for complexity, systemic diseases.The key to modernization of TCM was the modernization of TCM theory.Molecular compatibility theory, was the inevitable outcome of the development of The Times, which would promote the prosperity of Chinese medicine.关键词：molecular compatibility;modern Chinese medicine(molecular Chinese medicine);network pharmacological;complex diseases
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发布时间：2024-01-04 - 摘要：To study the methods of grade classification in Gastrodia elata pieces.The combined traditional and modern methods were used to evaluate G. elata pieces comprehensively based on the study of documents & origin investigation, and then classify the grade. According to the investigation, there have not been the grade of G. elata Bl. pieces but the one of G. elata materials. The same situation is also appeared in enterprises, hospitals and drugstore.There are only two grades of G. elata Bl. pieces. One is the first,and the other is the commom. The processing pieces grade is only for G. elata growed in winter. It is good for improving the quality of G. elata.pieces and achieving the aim ‘high quality with high price’ according to classify the grade comprehensively. This study can provide ideas and reference for other similar study on Chinese herbal pieces.关键词：Gastrodia elata;pieces;grades;quality evaluation
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