最新刊期
卷 23 ， 期 16 ， 2017
- 摘要：To review the development of connotation of Pharmaceutical Botany,and analyze the trends of teaching reform on Pharmaceutical Botany.Pharmaceutical Botany is the subject for the morphology and microstructure,classification and identification,growth and development,formation and variation of active ingredients in medicinal plants and guides the exploitation of new resources of medicinal plants.The trends of teaching reform on Pharmaceutical Botany is to cut the content of taxonomy and increase the basic knowledge of medicinal plant resources.The textbook trend is to be printed in color.Connotation,teaching content and teaching material form of Pharmaceutical Botany should be in advance with times.关键词：Pharmaceutical Botany;teaching;plant morphology;classification and identification;growth and development;active ingredients;new resources
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发布时间：2024-01-04 - 摘要：The blended teaching combines the advantages of traditional classroom teaching and online teaching,which is an important direction for reform of traditional Chinese medicine (TCM) higher education.The first use of online teaching in the course of Pharmaceutical Botany of Shandong University of TCM,through the planning of teaching content,design of knowledge points,selection of teaching methods,recording of online teaching video,the establishment of evaluation system and other aspects,we make full use of the complementary advantages of online learning and classroom teaching,to meet the needs of students' autonomous learning and improve their cognitive effect.At the same time,the overall level of teaching team was also improved.The reform of the blended teaching model will have some enlightenment and radiation effect on the construction of related courses.关键词：blended teaching model;ubiquitous learning;Pharmaceutical Botany;online school
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发布时间：2024-01-04 - 摘要：To cultivate applied talents with high-quality is the goal of our school as a local college and university in Guizhou province.Pharmaceutical Botany is an important part of curriculum system in Chinese materia medica and its field practice teaching is one of the characteristics of teaching,which lasted more than 30 years.Through the collation,this paper summarizes our contents,experiences and methods of field practice teaching,and puts forward our achievements of the local talent cultivation.It also puts forward the ideas and measures of educational reform of Pharmaceutical Botany,which will be of great significance to further improve the quality of teaching and the quality of students.关键词：applied talents;field practice teaching;Pharmaceutical Botany
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发布时间：2024-01-04 - 摘要：Pharmaceutical Botany is an important basic course for Chinese medicine and pharmacy,which including theory,experiment,practice training and other teaching links.Field practice is an important link in the curriculum system and the best combination of theory and practice,which can not only enable students to acquire knowledge,master skills,improve their comprehensive quality,and to stimulate interest in learning,complete the teaching task well.But the field practice has many contents without enough time,after many years of exploration and reform in the field practice of Pharmaceutical Botany,the authors explore a set of more scientific and perfect teaching methods.In this paper,the traditional method of diagnosis of disease was adopted,the plants were classified by the method of "look,listen,question and feel the pulse".This method can get multiplier effect on improving the level of teaching,the interests of scholars and enhancing the effectiveness of field teaching.关键词：look-listen-question-feel;Pharmaceutical Botany;practice;application;field teaching
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发布时间：2024-01-04 - 摘要：The massive open online courses (MOOC) is concerned by domestic and foreign learners in recent years.In order to improve the teaching effect of Pharmaceutical Botany and explore new mode and methods of teaching,the Chinese university MOOC platform provides Pharmaceutical Botany course for teachers and students.Through 8-week learning,the learners can know the compositions of this course well,and realize new learning mode on teaching evaluation,tests,and interacts between teachers and students.Meanwhile,the teachers of Pharmaceutical Botany can be inspired from MOOC,which would help them to think the improvement of teaching mode and methods and carry out inquiry teaching.In addition,the students' independent learning capacity,learning interest and learning efficiency can also be improved.关键词：Pharmaceutical Botany;massiveopenonlinecourses;learningpractice;teachingenlightenment
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发布时间：2024-01-04 - 摘要：Pharmaceutical botany was basic course to the majors of the resources and development,traditional Chinese medicine in our school.The problems to be solved in the process of teaching and learning were too many knowledge points,scattered,difficult to form a standardized knowledge system,cultivating students' learning interest and professional identity.The contents design of teaching and learning could update students' learning ideas,master knowledges of the points,lines and surface,and cultivate students' attitudes,feelings and values.The teaching contents design with the points to line and surface was been used in Pharmaceutical Botany,and we hope to optimize the instructional design and improve the teaching quality of Pharmaceutical Botany.关键词：basic course;instructional design;Pharmaceutical Botany;professional knowledge;values
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发布时间：2024-01-04 - 摘要：With more than 10 years of exploration experience,the authors made remarkable achievements in teaching reforms of Pharmaceutical Botany.We established the overall direction with practice as the center and improvement of student practice level as the goal,information technology teaching was used in a large area in teaching of Pharmaceutical Botany for guiding students to use information learning tools and platforms in teaching of Pharmaceutical Botany,among varieties of reform measures,we put emphasis on new proceedings of information technology,such as smartphones and "internet+",and these methods successfully roused students' interest in the study of this course and in the identification of important medicinal plants,improved their positivity in learning initiative and innovative.Before and after the reform,the degree of the students' enthusiasm for the course,the ability to identify the plant and the capability of comprehensive knowledge utilization has been significantly improved.This training was effective,and the students we nurtured meet the requirements of higher (vocational) education.关键词：Pharmaceutical Botany;educational reform;smartphone;“internet+”
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发布时间：2024-01-04 - 摘要：Pharmaceutical Botany is a kind of subjects linking theory with practice,practical teaching plays an important role in the course of Pharmaceutical Botany.Traditional teaching mode was single,which was not suitable for this course.Based on the establishment of a multi-level platform for practice teaching of Pharmaceutical Botany,including design of comprehensive experiments,application of interactive microsystem in experimental teaching,improvement of teaching method of problem-based learning (PBL),the construction of network teaching platform,establishment of the practical skills in the campus and improvements of the field practice.Integrating many kinds of education information and teaching resources together to further improve the quality and effect of the practice teaching of Pharmaceutical Botany.The traditional teaching method,teaching content and teaching mode of Pharmaceutical Botany are explored and reformed,which can provide practical reference for the establishment of new teaching mode in the end.关键词：Pharmaceutical Botany;multiplicity;practice teaching;platform construction
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发布时间：2024-01-04 - 摘要：With the great support and development of the traditional Chinese medicine(TCM),the TCM propaganda programs,film and television works are constantly emerging.Appropriate use of the above video teaching can effectively stimulate students' learning interest and potential to improve the teaching effect of Pharmaceutical Botany.This article actively discusses the film and television works,and they can practice to the teaching of this course,we selected the teaching list of film and television works for this course.This paper probes into the problems in the process of video projection teaching,such as collection and arrangement,simple processing of video,teaching design,etc.Finally,we look forward to working together with peers to explore and optimize the teaching mode and the roles of film and television works.关键词：film and television works;Pharmaceutical Botany;teaching methods
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发布时间：2024-01-04 - 摘要：Pharmaceutical Botany is a professional basic course based on practical teaching,the knowledge of this course is complicated.In order to improve the quality of experimental teaching,we make use of smartphone to all aspects of teaching,including technical standardization operation,photos shooting in plant,interactive communication,data searching and so on.Through the comparison in practice assessment results,we determined that the new attempt can effectively stimulate the enthusiasm of students and improve the students' ability of operating,analyzing and solving problems.关键词：Pharmaceutical Botany;experimental teaching;smartphone;massive open online courses
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发布时间：2024-01-04 - 摘要：Objective: This study is aimed to clone the key enzyme 1-deoxylated xylose-5-phosphate synthase (DXS) genes and analyze its tissue-specific expression in Atractylodes lancea. Method: The full-length cDNA sequence was cloned from the DXS gene fragment based on transcriptome sequencing data of A. lancea ge nerated in our previous study. The expression levels of DXS gene in different tissues of A. lancea were detected by Real-time PCR, with tublin as the reference gene. Result: The full-length cDNA sequence of DXS gene is 2 151 bp, encoding 716 amino acids, which has been deposited in GenBank (accession number KY659208). Results of phylogenetic analysis showed that the sequence had high homology with DXS gene of other compositae plants, such as Stevia rebaudiana. Real-time PCR analysis showed that the DXS gene was mostly expressed in leaves, followed by flowers, rhizome and roots. Conclusion: Full-length cDNA sequence of DXS gene in A. lancea has been obtained for preliminary bioinformatics analysis, revealing differential expression in different tissues. The results will provide a groundwork for studying the function of DXS in terpenoid biosynthesis of A. lancea.关键词：Atractylodes lancea;1-deoxy-xylose-5-phosphate synthase;gene cloning;sequence analysis;terpenoids
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发布时间：2024-01-04 - 摘要：Objective: To study on the expression of genes involved in the biosynthesis of terpenoid secondary metabolism, and explore the functional genes in Aconitum carmichaelii based on transcriptomics sequencing analysis of six A. carmichaelii organs (including main root, lateral root, stem, leaves, flower and fruit). Method: Illumina Hiseq 2500 was used to detect the sequencing and assemble into unigennes. By comparing with public database, the genes were annotated and differential expressed genes were obtained. Subsequently, Real-time PCR method was used to verify the key genes involved in the terpenoid secondary metabolism biosynthesis. Result: Total 156 967 635 Clean Reads (28 254 174 300 bp) were obtained, and 103 337 unigenes were obtained after sequence merging. Homology analysis of nucleotide and protein sequences indicated that 37.31% (38 554 unigenes) had different degree of homology with other creatures. In analysis of the functional classification and metabolic pathway (KEGG), 158 unigenes (encoding 5 enzymes) were involved in terpenoid synthesis. Conclusion: These genes will provide the basic data for the terpenoids biosynthetic pathway and molecular mechanism of A. carmichaelii.关键词：Aconitum carmichaelii;transcriptome;terpenoid;secondary metabolism biosynthesis
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发布时间：2024-01-04 - 摘要：Objective: To explore the antibacterial activity differences of various extracts of Coptidis Rhizoma from the perspective of biothermodynamics, and to provide a new idea and evidence for the study on active components of Coptidis Rhizoma. Method: Microcalorimetry was used to establish biothermodynamics of Escherichia coli (EC), Staphylococcus aureus (SA) and Shigella dysenteriae (SD) on Luria-Bertani (LB) medium under the sterile conditions of 37℃ with an inoculum concentration of 1×105cell/mL. This method was used to plot growth thremogram curves of these three bacteria upon the treatments by aqueous extracts, alkaloids and non-alkaloid constituents of Coptidis Rhizoma. The differences in the growth metabolism of these bacteria upon various treatments were assayed on the basis of corresponding biothermodynamics parameters (mainly including growth rate constant (k), growth inhibition ratio (I), inhibition ratio in half of drug concentration (IC50), maximum heat output (Pmax) and the time to reach Pmax(tmax). Result: The aqueous extracts and alkaloids significantly inhibited the growth of the three bacteria, and all the orders were alkaloids>aqueous extracts; non-alkaloid constituents had no significant inhibitory effects, and even had promotion effects to these three bacteria. The order of the antibacterial ability was SA> SD> EC for the same extraction part. Conclusion: In summary, the microcalorimetry analysis reveals that in Coptidis Rhizoma, the main active components are alkaloids that display the strongest antibacterial activities against SA.关键词：biothermodynamics;microcalorimetry;Coptidis Rhizoma;aqueous extract;alkaloids constitutents;monalkaloids constituents
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发布时间：2024-01-04 - 摘要：Objective: To study on chemical constituents from the seeds of Celastrus angulatus by means of modern separation method and spectrum technology, and provide experimental data for its research, development and utilization. Method: Dry seeds of C. angulatus were extracted by petroleum ether for 3 times. Its extracts were then further extracted by 90% methanol solution for 3 times. From the methanol layer, the compounds were isolated by silica gel column chromatography and petroleum ether-ethyl acetate solvent system (10:1-2:8) was used for gradient elution. The eluent received decompressed drying and was further separated and purified by preparative HPLC with methanol-H2O solvent system, and finally purified by recrystallization. The compounds were identified on the basis of physic-chemical constants and spectral analysis. Result: Six compounds were obtained and identified as 1β, 15-diacetoxy-8β, 9β-dibenzoyloxy-β-dihydroagarofuran (1), angulatueoid H (2), angulatinoid Ⅱ (3), angulatinoid Ⅲ (4), 1β,15-diacetoxy-8α-hydroxy-9β-benzoyloxy-β-dihydroagarofuran (5) and 1β,2β,8α-triacetoxy-9β-benzoyloxy-β-15-nicotinoyloxy-β-dihydroagarofuran (6) respectively, with their NMR data detected through 1D (1H, 13C-NMR and DEPT) and 2D NMR (1H-1H COSY, HSQC, HMBC and NOESY) techniques. Conclusion: Compounds 1, 3 and 5 were obtained from C. angulatus for the first time. Compound 4 was obtained from the nature for the first time.关键词：Celastrus angulatus;chemical constituents;sesquiterpenes
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发布时间：2024-01-04 - 摘要：Objective: To study on the chemical compositions of medium polarity part in 70% ethanol extract of Millettia nitita var.hirsutissima. Method: Silica gel column, Sephadex LH-20 column, reverse phase chromatography technology and preparative chromatography were used for separation and purification, and their chemical structures were identified according to the nuclear magnetic resonance spectrum results. Result: The 15 compounds were isolated from 70% ethanol extract by chromatography and their structures were identified as formononetin (1), 8-methylretusin (2), astragaluquinone (3), salicylic acid (4), calycosin(5), genistein (6), wistin (7), 3',4',7-trihydroxy flavone (8), butein(9), sissotrin(10), mncodianin F (11), ononin (12), vitexin (13), 4',8-dimethoxyl-7-O-β-D-glucopyranosyl isoflavone (14), and (2S)-1-O-heptatriacontanoyl glycerol (15). Conclusion: Compounds 2 and 11 were isolated from this plant for the first time; compounds 3, 8, 9, 13 and 14 were isolated from this genus for the first time.关键词：Millettia nitita var.hirsutissima;chemical component;separation and identification;flavanes;isoflavone
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发布时间：2024-01-04 - 摘要：Objective: The anti-influenza virus activity of Galla Chinensis extracts was evaluated by an influenza virus neuraminidase (NA) inhibition assay. The constituents in the active extract were identified using ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) major constituents were used to further evaluate the NA inhibitory activity. Method: Using Neuraminidase Inhibitors Screen Kit to screen the four extracts of Galla Chinensis with anti-influenza virus activity. The chromatographic separation was performed on a C18 column with a gradient elution of methanol-aqueous formic acid 0.1%. The mass spectrometer equipped with electron spray ionization source was used as detector and the data was collected under the negative ion modes. Result: The results showed that four extracts of Galla Chinensis both presented NA inhibition activity. The ethyl acetate extract (EA) and the ethanol extract (E) presented strong. NA inhibitory activity with inhibition ratio of 69.64% and 78.24%, respectively. Eighteen constituents were characterized in these two active extracts by UPLC-Q-TOF-MS analysis,and three constituents (pentagalloyl glucose,ellagic acid, gallic acid) exhibited high activity against the influenza virus,IC50 values of 46.15,143.42,249.51 μmol ·L-1 respectively. Conclusion: Galla Chinensis has significant influenza virus neuraminidase inhibitory activity. Eighteen phenolic acids constituents were identified by UPLC-Q-TOF-MS. The results indicate that Galla Chinensis contains phenolic acids constituents as inhibitors of NA and provide a basis for anti-Influenza Virus Activity of Galla Chinensis study on its mechanism.关键词：Galla Chinensis;neuraminidase inhibition;chemical constituents;anti-influenza activity;UPLC-Q-TOF-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents and analgesic activity of Primula denticulata ssp. sinodenticulata and to lay the foundation for the development of Primula plants in the future. Method: The major compounds from 85%ethanol extract of P. denticulata ssp. sinodenticulata were isolated by solvent extraction, recrystallization method, as well as various column chromatography methods including silica gel, RP-18, and Sephadex LH-20 column chromatography. The structures of the compounds were identified by spectral technique combined with TLC method, and their analgesic activity of samples and selected compounds from P. denticulata ssp. sinodenticulata was evaluated by using acetic acid induced writhing method and hot plate method. Result: Seven monomeric compounds were obtained from ethyl acetate and n-butanol fractions and identified as 2,2'-dihydroxychalcone (1), 5-hydroxyflavone (2), 3'-hydroxyflavone (3), quercetin 3-O-(2,6-di-O-β-D-glucopyranosyl)-β-D-glucopyranoside (4), riccardin C (5), primeverin (6), and hexabecanoic acid (7). All of the compounds were obtained from this plant for the first time. Compounds 2, 3, and 7 could reduce the times of writhing in mice induced by acetic acid, but could not increase the pain threshold of mice induced by hot plate. Conclusion: P. denticulata ssp. sinodenticulata was rich in flavonoids and phenolic compounds. Some of the flavonoids had peripheral analgesic activity, and the analgesic effect was similar to or even better than that of aspirin.关键词：Primula denticulate ssp.sinodenticulata;chemical constituents;flavonoids;analgesic activity
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发布时间：2024-01-04 - 摘要：Objective: According to observation of the anti-cancer effect of bufadienolides from cinobufacin injection in vivo and in vitro and the anti-pancreatic cancer cells of bufadienolide monomers in vitro,to indentify the foundation of bufadienolides on anti-pancreatic cancer. Method: Modern chromatographic separation technology was adopted to obtain bufadienolides from cinobufacin injection,HPLC-DAD-FT-ICR-MS was employed to analysis and identify the main monomers of bufadienolides.Anti-cancer activity of bufadienolides was screened by in vitro cells,then setting up the pancreatic cancer animal model in nude mice in vivo for evaluating the efficacy of bufadienolides,the anti-pancreatic cancer activity of the main bufadienolide monomers in vitro was screened. Result: IC50 of bufadienolides from cinobufacin injection on hepatocellular carcinoma cells(BEL7402),gastric carcinoma cells(BGC823) and pancreatic cancer cells (SW1990 and MIA PaCa-2) in vitro were (0.47±0.03),(0.06±0.01),(0.06±0.02),(0.11±0.03) g· L-1,respectively.LD50 of bufadienolides was 20 mg·kg-1 and the treatment effect on SW1990 nude mice in vivo was close to gemcitabine.Gamabufotalin,arenobufagin and hellebrigenin had obvious inhibitory effect on SW1990 and BxPC-3 cells,which were isolated and identified from bufadienolides. Conclusion: Bufadienolides from cinobufacin injection shows almost the same inhibition rate on pancreatic cancer cells as gastric cancer in vitro,and it has apparently treatment effect on human pancreatic cancer bearing xenograft nude mice in vivo.Gamabufotalin,arenobufagin and hellebrigenin from bufadienolides show significantly inhibition on pancreatic cancer cells in vitro.关键词：bufadienolides;anti-cancer;anti-pancreatic cancer;in vivo experiment;in vitro screening;cinobufacin injection;arenobufagin
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发布时间：2024-01-04 - 摘要：Objective: To study the decoction pieces and water extracts of Genkwa Ramulus by using high performance liquid chromatography (HPLC) and Tri-Step infrared identification method. Method: The 10 batches of crude drugs of Genkwa Ramulus were collected; their decoction pieces and water extracts were prepared separately. Tri-Step infrared spectroscopy was used to analyze various batches of decoction pieces and water extracts; HPLC was used to determine the content of daphnetin. The data were analyzed to determine the characteristic spectrum of Genkwa Ramulus decoction pieces and water extracts, and then the component differences and similarities between these two forms were investigated. Result: From the one-dimensional spectroscopy, Genkwa Ramulus decoction pieces and water extracts showed maximum absorption at 1 055 and 1 073 cm-1 respectively. From the second derivative spectroscopy, the results of Genkwa Ramulus water extracts (1 707, 1 650 and 1 624 cm-1) were asically consistent with those of daphnetin (1 709, 1651 and 1 625 cm-1). Two-dimensional correlation infrared spectroscopy showed that both Genkwa Ramulus decoction pieces and water extracts had three obvious auto-peaks at different regions. From HPLC, the results showed that the content of daphnetin in the water extracts was significantly higher than that in decoction pieces, indicating that this kind of components can be effectively enriched after water extraction. Conclusion: Tri-Step infrared spectroscopy provided the overall composition information of Genkwa Ramulus decoction pieces and water extracts; two dimensional infrared spectroscopy can be used for the identification of these two forms; and HPLC was used to detect the content of main effective compositions and further explain the changes of compositions.关键词：Genkwa Ramulus;decoction pieces;water extract;infrared spectroscopy;identification
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发布时间：2024-01-04 - 摘要：Objective: To develop a certified reference material (CRM) of β-sitosterol according to the Directives for CRM General and Statistical Principle for Certification (GB/T 15000.3-2008) based on the CRM project approved by Standardization Administration of the People's Republic of China (SAC). Method: β-Sitosterol was obtained from the dried seeds of Sinapisalba by means of silica gel column chromatography and recrystallization. The structure of β-sitosterol was confirmed by elemental analysis (EA), UV, IR, MS, NMR and XRD. Thin layer chromatography was carried out for the identification, and after sample of β-sitosterol was divided into 300 bottles with 10 mg per bottle, the homogeneity test, stability test, and cooperative certification were carried out by high performance liquid chromatography with evaporative light-scattering detector (HPLC-ELSD). Result: The homogeneitywas good within 95% confidence interval. The stability was good within 24 months storage period at 0-8℃. The certified purity value was 95.41%with an expanded uncertainty of 0.30% (k=2). The CRM of β-sitosterol complied with the technical requirement of a series of GB/T 15000.3 guides about CRM, and it was checked and accepted by SAC. Conclusion: The national CRM of β-sitosterol was developed for the first time, which can be used in the content determination, method validation, testing and quality control of related products.关键词：β-sitosterol;CRM;homogeneity;stability;certification;uncertainty
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from Dischidia chinensis. Method: The air-dried powder of the whole plant of D.chinensis (5kg)was extracted with 95% ethyl alcoholthree times at room temperature to give crude extract. Compounds were isolated and purified by using MCI gel,silica gel, Sephadex LH-20 and their structures were elucidated by physicochemical and organic spetra. Result: Ten compounds were obtained and identified as β-amyrin (1), lupeol(2), β-amyrin acetate(3), 3-epifriedelinol(4), vanillin(5), 4-hydroxy-3,5-dimethoxybenzaldehyde (6), 2-hydroxy-1-(4-hydroxy-3-methoxyp-henyl)propan-1-one(7), 1-(4-hy droxy-3-methoxyphenyl)-1-methoxypropan-2-ol(8), pavonisol(9), and S (+)-dehydro-vomifoliol(10), respectively. Conclusion: The compounds 1-10 were obtained from the D. chinensis for the first time.关键词：Dischidia chinensis;chemical constituents;isolation and identification;eghyl acetate extract
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antidiabetic effect of Acanthopanax senticosus total glycosides extract based on GC-MS metabonomics and to explore its mechanism. Method: Diabetic mice model was established by intraperitoneal injection of streptozotocin and alloxan,multivariate statistical analysis was employed to analyze serum metabolites,NIST 11 database and authentic standards were used to analyze and identify differential metabolites,and MetPA was employed to analyze the corresponding metabolic pathways. Result: The diabetic mice model was duplicated successfully.After administration of A.senticosus total glycosides extract,the levels of metabolites in the model group were detected,hexadecanoic acid,octadecanoic acid,linoleic acid,tyrosine,phenylalanine,aspartic acid and serine showed decrease;alanine,lactic acid,citric acid,glutamine,valine,iso(leucine),β-hydroxybutyric acid and glycine showed increase,which indicated a tendency towards normality.Pathway enrichment analysis by MetPA displayed that these metabolites were mainly involved in six metabolic pathways which therefore were closely related to diabetes. Conclusion: A.senticosus total glycosides extract displays a significant hypoglycemic effect,which may be achieved by regulation of energy metabolism,amino acid metabolism,amino acid biosynthesis and organic acid metabolism.关键词：Acanthopanacis Senticosi Radix et Rhizoma seu Caulis;total glycosides;antidiabetic effect;diabetes;metabonomics
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of combined prescription of Linggui Zhugan Tang (LGZGT) and Yinchenhao Tang (YCHT) on Nrf2/ARE signaling pathway in rats with non-alcoholic steatohepatitis (NASH) from the perspective of oxidative stress, and investigate its mechanism for treating non-alcoholic steatohepatitis. Method: SPF grade SD rats were randomly divided into normal control group, model group, sulforaphane group (0.5 mg·kg-1), LGZGT group (3.465 g·kg-1),YCHT group (3.465 g·kg-1) and LGZGT+YCHT combination group (6.93 g·kg-1). High-fat diet was given to establish NASH models, and the treatment was given at the same time for 8 weeks. Then the serum was harvested separately to detect the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), and low density lipoprotein (LDL-C); part liver tissues were taken for HE staining; Western blot and reverse transcription PCR were used respectively to detect the protein and mRNA expression levels of Kelch-like ECH-associated protein 1 (Keap1), nuclear factor E2-related factor 2 (Nrf2), quinone oxidoreductase-1 (NQO1), and heme oxygenase-1 (HO-1). Result: As compared with normal control group, the levels of TC, TG, HDL-C and LDL-C in blood lipid and ALT, AST levels in serum were increased;liver fat became obvious; the expression level of Keap1 in liver was decreased; and, Nrf2, NQO1, HO-1 protein and mRNA expression levels were increased in the model group (P <0.05).As compared with the model group, the levels of TC, TG, HDL-C and LDL-C in blood lipid and ALT, AST levels in serum were decreased in various treatment groups, liver pathology was also improved to some extent; and Nrf2, NQO1, HO-1 protein and mRNA expression levels were increased in treatment groups (P <0.01, P <0.05) except YCHT group. However, the levels of Keap1 didn't show obvious differences among the treatment groups. Conclusion: LGZGT and its combination formula may improve oxidative stress to prevent and treat NASH via activating Nrf2/ARE signaling pathway in the liver.关键词：Linggui Zhugan Tang;Yinchenhao Tang;non-alcoholic steatohepatitis;oxidative stress;Wenyun Qingli method
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发布时间：2024-01-04 - 摘要：Objective: To predict and investigate the molecular mechanism of active principle region of Yangxing Tongmai formula (apr-YTF) in inducing bone marrow mesenchymal stem cells (BMSCs) myocardial differentiation based on miRNA level. Method: BMSCs from acute myocardial infarction (AMI) rats of blood-stasis model were extracted and cultivated. Then apr-YTF serum was used as experimental group and negative control serum was used as control group, respectively. Total RNAs were extracted and differently expressed miRNAs were detected by expression profile chip for hierarchical clustering analysis. Bioinformatics method was used to predict target genes and analyze the functions of differential miRNAs regulation. Result: There were a total of 26 differently expressed miRNAs, 8 were up-regulated, and 18 were down-regulated. In screening and predicting, 100 target genes were involved in 19 kinds of biological processes, 11 kinds of cellular components, and 10 kinds of molecular functions. Axon guidance, Transcriptional misregulation in cancer, and AMPK signaling pathway were enriched. Rno-miR-93-5p, rno-miR-204-5p, rno-miR-128-3p were the center of the molecular network, and rno-miR-204-5p was down-regulated significantly. Conclusion: The results showed that rno-miR-204-5p, rno-miR-93-5p and Axon guidance pathway may play a role in the differentiation process of BMSCs into cardiomyocytes induced by apr-YTF.关键词：active principle region of Yangxin Tongmai formula;bone marrow mesenchymal stem cells;acute myocardial infarction;miRNA;bioinformatics
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of ‘hoop circumference’(Hongzhongxiao tincture) on the gene expression levels of interleukin-10 (IL-10) and interleukin-1β (IL-1β) in the tissues around the wound of rats with skin abscess under the guidance of the theory of ‘defended field’. Method: The 32 rats were divided into blank group, model group, Mupirocin ointment group and Hongzhongxiao tincture group. Except the rats in blank group, all the rats in other three groups received subcutaneous injection of Staphylococcus aureus 1 mL (3×109-5×109 cfu·mL-1) to establish subcutaneous abscess models. The rats in model group received normal saline for treatment. Mupirocin ointment 2 g dressing was given in Mupirocin ointment group; and 2 mL Hongzhongxiao dressing was given in Hongzhongxiao tincture group, 1 cm over the swelling range, 1 time a day. The tissues 1, 2, 3 cm around the wound were taken on day 3, 7, 14 and 18 of treatment to observe the healing time and wound abscess limitation time; in addition, the transcription levels of IL-10 and L-1β mRNA were detected. Result: As compared with model group, Hongzhongxiao tincture group could shorten the time of the abscess and wound healing time, (P <0.05, P <0.01); however, there was no significant difference between Hongzhongxiao tincture group and Mupirocin ointment group. The IL-10 level was increased in Hongzhongxiao tincture group, and higher than that in the model group and Mupirocin ointment group during the treatment; while IL-1β level in Hongzhongxiao tincture group was lower than that in the Mupirocin ointment group and model group at the early treatment stage (P <0.05, P <0.01). Conclusion: The Hongzhongxiao tincture can increase IL-10 level, and inhibit the expression of IL-1β in the tissues in the early stage of abscess formation, thus reducing the local inflammatory reaction of the abscess effectively, promoting the limitation of abscess and wound healing, and providing conditions for the formation of the "defended field".关键词：defended field;the method of hoop circumference;rat skin abscess;Hongzhongxiao tincture;interleukin-10(IL-10);interleukin-1β(IL-1β)
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effects of Erzhiwan by prophylactic and therapeutic administrations for the liver cytothesis obstacle in rats after injury, and investigate its mechanism. Method: The 50 Wistar rats were randomly divided into 5 groups:normal group, model group, Erzhiwan prophylactic group (6.48 g·kg-1), Erzhiwan therapeutic group (6.48 g·kg-1), and Rapamycin group (1 mg·kg-1). In normal group, partial hepatectomy (PHx) was conducted to establish PHx models; in the other groups, animals received intragastric administration of 2-acetylaminofluorene/partial hepatectomy (2-AAF) for 7 days; meanwhile, in the Erzhiwan prophylactic group, those rats were administrated with Erzhiwan for 7 days, and on the 8th day, PHx was conducted to establish compound models of liver cytothesis obstacle after injury (2AAF/PHx models). 24 h after surgery, the rats in the prophylactic and therapeutic groups were administrated with Erzhiwan for three days. After rats were killed, the index of liver weight was calculated; liver pathomorphological changes were observed; and the levels of alanine transarninase (ALT), aspartate aminotransferase (AST) and albumin in serum were detected by using automatic biochemical analyzer. In addition, liver apoptosis and Caspase-3 expression levels were analyzed by flow cytometry. Result: As compared with the normal group, the index of liver weight, albumin level and count of living cell(LC) were significantly decreased, while ALT and AST levels, non-viable non-apoptotic cell(NVNAC),viable apoptotic cell(VAC),non-viable apoptotic cell(NVAC) and Caspase-3 expression levels were significantly incereased in model group (P <0.05,P <0.01). As compared with the model group, the index of liver weight, level of albumin and count of LC were increased, however, the levels of ALT, AST, NVNAC,VAC,NVAC and Caspase-3 expression levels were decreased in both Erzgu pill prophylactic and therapeutic groups (P <0.05,P <0.01). Meanwhile pathological observation hinted that injured livers were improved markedly. Conclusion: Erzhiwan remarkably inhibited liver apoptosis, and decreased Caspse-3 expression of 2-AAF/PHx rats to remit damaged liver function. So Erzhiwan play a favorable and protective role in treatment of cytothesis obstacle of damaged liver in rats关键词：Erzhiwan;prevent;cytothesis obstacle of damaged liver;therapeutic effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-liver fibrosis effect of Ganlong capsule combined with compound glycyrrhizin glucoside on human hepatic stellate cells by detecting the content of type I collagen (Col-I), type III procollagen (PCIII), type IV collagen (IV-C), laminin (LN), hyaluronic acid (HA), transforming growth factor-β1 (TGF-β1) and expression levels of Col-I, matrix metalloproteinase inhibitor 1 (TIMP-1) mRNA in human hepatic stellate cell line (LX-2). Method: LX-2 cells were cultured in vitro, and the logarithmic phase cells were divided into compound glycyrrhizin glucoside treatment groups (3.125, 1.562, 0.781 g·L-1), blank control group, Ganlong capsule (0.938, 0.469, 0.235 g·L-1) combined with compound glycyrrhizin glucoside (3.125, 1.562, 0.781 g·L-1) groups. Then enzyme-linked immunosorbent assay (ELISA) method was used to detect the content of four markers of liver fibrosis (PCIII, IV-C, LN, HA) and the levels of Col-I and TGF-β1; the expression levels of Col-I and TIMP-1 mRNA were detected by reverse transcription-PCR (RT-PCR). Result: As compared with compound glycyrrhizin glucoside treatment group, combined drug use could decrease the content of Col-I, HA at 24 h and 48 h (P <0.05), and reduce the content of LN, HA, Col-I, PCIII, TGF-β1 at 72 h (P <0.05). As compared with the blank control group, the combined drug use can significantly decrease the content of TGF-β1 (P <0.05, P <0.01). As compared with compound glycyrrhizin glucoside treatment group, the combined drug use could significantly decrease the expression of TIMP-1 mRNA (P <0.05) in time-dependent manner; and as compared with the blank control group, the combined drug use showed the inhibition effect for Col-I mRNA at 24 h (P <0.05), but the expression was increased at 72 h. Conclusion: Ganlong capsule combined with compound glycyrrhizin glucoside can decrease the content of LN, HA, Col-I, TGF-β1 and inhibit the expression of Col-I and TIMP-1 in LX-2 cells, and the effect was better than that of compound glycyrrhizin glucoside treatment group. Ganlong capsule could enhance the anti-fibrosis effect in vitro of compound glycyrrhizin glucoside.关键词：Ganlong capsule;compound glycyrrhizin glucoside;durg combination;extracellular matrix;hepatic fibrosis
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of baicalein on ultraviolet B(UVB) induced-human skin keratinocyte (HaCaT) cell light aging and p38 mitogen activated protein kinase (p38MAPK) signaling pathways. Method: The 1×10-11, 1×10-10, 1×10-9, 1×10-8, 1×10-7, 1×10-6, 1×10-5, 1×10-4, 1×10-3 mol·L-1 baicalein were used on HaCaT cells, and the best effective concentration was determined by methyl thiazolyl tetrazolium(MTT) method. In the preliminary experiments, UVB with radiation intensity of 0.5 mW·cm-2 was selected, and with radiation time for 5 min to establish light aging models. Then the best effective concentration was used on the light aging models. MTT method was used to detect the cells activity in each group; superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT) and malondialdehyde(MDA) kits were used to detect SOD, GSH-Px, CAT activity and MDA content respectively. Real-time quantitative PCR and Western blot were used respectively to detect mRNA and protein expression levels in each group. Result: 1×10-7, 1×10-6, 1×10-5 mol·L-1 baicalein were selected as the best effective concentrations. As compared with the blank group, UVB had no significant proliferation effect on HaCaT cells. As compared with UVB group, 1×10-7, 1×10-6, and 1×10-5mol·L-1 baicalein had no obvious effect on the proliferation of light aging models. As compared with the blank group, SOD, GSH-Px and CAT activities were significantly decreased while MDA contend was increased in UVB group (P <0.01). As compared with UVB model group, 1×10-7, 1×10-6, 1×10-5 mol·L-1 baicalein could increase SOD, GSH-Px and CAT activities, and decrease MDA contend (P <0.05, P <0.01). As compared with the blank group, the expression levels of tumor necrosis factor (TNF)-α mRNA as well as TNF-α and p38 protein were significantly increased in UVB group (P <0.01). As compared with UVB model group, 1×10-7, 1×10-6, 1×10-5 mol·L-1 baicalein could decrease the expression levels of TNF-α mRNA as well as TNF-α and p38 protein (P <0.05, P <0.01). Conclusion: Baicalein has the protective effect on UVB-induced cell light aging, and its mechanism is mainly associated with tenhancing GSH-Px,SOD and CAT activities, blocking p38MAPK signaling pathway, and inhibiting inflammatory cytokines.关键词：baicalein;ultraviolet B;HaCaT;light aging;oxidase;p38MAPK signaling pathway
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发布时间：2024-01-04 - 摘要：Objective: To explore the mechanism of Qutan Huoxue granules in the treatment of non-alcoholic fatty liver disease (NAFLD) in rats. Method: The 40 SD rats were randomly divided into normal group (n=6) and model group (n=34). NAFLD rat models were induced by high fat diet, and after successful modeling, the successful modeling group (n=30) were further randomly divided into 5 groups, 6 in each group:model group, Qutan Huoxue granules high, middle and low dose groups (10, 5, 2.5 g·kg-1) and SB203580 group (3 mg·kg-1). Intragastric administration of Qutan Huoxue granules and intraperitoneal injection of p38MAPK inhibitor SB203580 were given respectively for 4 weeks. Then the pathological changes of liver were observed by htoxylin-eosin(HE) staining and oil red O staining; Kits were used to detect the triglyceride (TG), total cholesterol (TC), aspartate transaminase (AST), and alanine transferase (ALT) levels in serum, and TG levels in liver tissues; the adipose tissue aquaporin7 (AQP7) and p38 mitogen activated protein kinase (p38MAPK) mRNA expression levels were detected by Real-time PCR; enzyme linked immunosorbent assay(ELISA) was used to detect the expression of free fatty acid (FFA) in liver, AQP7 and phosphorylated p38MAPK (p-p38MAPK) expression in adipose tissues. Result: As compared with the normal group, the model group showed an obvious hepatic steatosis and a significant increase in serum TG, TC, AST, ALT and liver TG, FFA levels, a significant decrease in the expression of AQP7 mRNA and AQP7 protein and an increase of p38MAPK mRNA and p-p38MAPK protein (P <0.05). As compared with the model group, Qutan Huoxue granules and SB203580 can reduce the degree of liver steatosis, and significantly decrease the serum TG, TC, AST, ALT and liver TG, FFA levels in NAFLD rats (P <0.05). The Chinese medicine groups and SB203580 group showed a significant increase in the expression of AQP7 mRNA and AQP7 protein and a decrease of p38MAPK mRNA and p-p38MAPK protein in adipose (P <0.05). Conclusion: Qutan Huoxue granules can reduce or reverse the liver steatosis, and the mechanism may be related to inhibiting the activation of p38MAPK, up-regulating the expression of AQP7 in adipose tissues, increasing plasma glycerol metabolism, reducing glycerol and FFA intake into liver, hereby reducing TG accumulation in liver.关键词：nonalcoholic fatty liver disease (NAFLD);adipose tissue;aquaporin7(AQP7);p38MAPK pathway;mechanism
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发布时间：2024-01-04 - 摘要：Objective: To explore the effects of compound Shenlu granule (FSG) on the apoptosis of CD34+ cells of lower-risk myelodysplastic syndromes (MDS) bone marrow based on p38 mitogen-activated protein kinase (MAPK) pathway. Method: The 60 patients with lower-risk MDS patients were randomly divided into FSG group and andriol treatment group,30 cases in each group. All of the patients were treated for 3 months. Changes of blood routine, rates of apoptosis of CD34+cells, p-p38, p38, mitogen activated protein kinase kinase(MKK)3/6, p-p53, Bcl-xl, B-cell lymphoma-2(Bcl-2) protein and p38 mRNA expression levels in bone marrow mononuclear cells (BMMNC) were observed before and after treatment. 7 cases from control group were age-matched patients with non-MDS cytopenia. Result: After treatment, the red blood cells count, platelet count and hemoglobin count were increased in FSG group (P <0.05, P <0.01); hemoglobin count was increased in Andriol group after treatment and there was no significant difference in the count of WBC, RBC and PLT. As compared with control group, apoptosis rate of CD34+cells and expression levels of p-p38,MKK3/6,p-p53 protein were higher, and expression of Bcl-xl was lower in FSG group (P <0.05,P <0.01); while there was no significant difference in p38, Bcl-2 protein expression and p38 mRNA expression. After FSG treatment, CD34+cell apoptosis rate and levels of p-p38,MKK3/6, p-p53 protein expression in FSG group were lower (P <0.05), p-p38 and Bcl-xl protein expression levels were higher than those in control group (P <0.05). There was no significant difference in the apoptosis rate of CD34+, p-p38, MKK3/6, p-p53, Bcl-xl protein expression in Andriol group before and after treatment. P-p38 protein expression and CD34+ cell apoptosis rate were positively correlated before treatment in control group (P <0.01), and the decrease in apoptosis rate of CD34+ was positively correlated with decrease in p-p38 protein expression after treatment in FSG group. Conclusion: FSG could inhibit the apoptosis of bone marrow CD34+cells by regulating the p38MAPK pathway, and improve the ineffective hematopoiesis of bone marrow.关键词：myelodysplastic syndromes;p38 mitogen-activated protein kinase (MAPK);compound Shenlu granule;cell apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effect of Shashen Maidong Tang combined with chemotherapy on the immune function and inflammatory reaction of patients with lung cancer of Qi and Yin deficiency, and investigate its mechanism of action. Method: The 110 patients with non small cell lung cancer in Zunyi Hospital of Traditional Chinese Medicine were randomly divided into treatment group and control group, 55 cases in each group. Both groups of patients were given with corresponding treatment measures. The patients in control group were treated with chemotherapy of gemcitabine+cisplatin, and the patients in treatment group were treated with Shashen Maidong Tang on the basis of the treatment in control group, 200 mL/time, 1 dose/d, one time in the morning and one time in the evening. 21 days were regarded as one treatment course, and they were treated for two consecutive courses in both groups. Serum CD4+, CD8+, CD4+/CD8+, natural killer cell (NK), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), effective rate, Kamofsky patients health status score (KPS), body mass change and adverse reaction incidence were observed and compared before and after treatment in two groups. Result: As compared with the conditions before treatment, CD8+, IL-6, and TNF-α levels were decreased, and the level of CD4+, CD4+/CD8+ were increased in both groups after treatment (P <0.05). After treatment, the CD8+, IL-6, TNF-α levels were lower, while CD4+, CD4+/CD8+ levels were higher in treatment group than those in control group (P <0.05); and there was no significant difference in the level of NK cells between two groups before and after treatment. After treatment, the effective rate of clinical treatment, KPS score and body mass stable rate were higher, and incidence of adverse reactions was lower in treatment group as compared with those in control group (P <0.05). Conclusion: Shashen Maidong Tang combined with chemotherapy can improve the immune function of patients with Qi and Yin deficiency type lung cancer, and reduce the inflammatory reaction in patients, with higher clinical efficacy and safety.关键词：Shashen Maidong tang;Qi and Yin deficiency syndrome;lung cancer;immune function;inflammatory reaction
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发布时间：2024-01-04 - 摘要：Objective: To explore the clinical efficacy of Ziyin Zhuyang Kangshuai decoction combined with western medicine for Yang insufficiency and water overflowing type chronic heart failure (CHF) and observe its effect on patients' oxidative stress and quality of life. Method: The 116 patients with CHF in our hospital from June 2014 to June 2016 were selected and divide into two groups according to random number table. Routine therapy was given in both groups, and patients in control group also received standard western anti heart failure drug; while the patients in observation group received Ziyin Zhuyang Kangshuai decoction based on the treatment in control group. The efficacy for traditional Chinese medicine (TCM) syndromes, cardiac function, echocardiographic index, the level of N-terminal pro-brain natriuretic peptide (NT-proBNP), oxidative stress, the overall score of Minnesota Living with Heart Failure Questionnaire (MLHFQ) and drug safety evaluation were recorded and compared between two groups. Result: After therapy for 3 months, overall effective rate of TCM syndromes and cardiac function was 90.6% and 84.9% respectively in observation group, significantly higher than 75.5% and 67.9% in control group (P <0.05). As compared with the conditions before treatment, the left ventricular ejection fraction (LVEF), mitral flow E/A, left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic diameter (LVEDD) and NT-proBNP level were significantly improved in both groups after treatment (P <0.01). The improvement in echocardiographic index and NT-proBNP in observation group was better than that in control group after treatment (P <0.01). As compared with the conditions before treatment, the level of plasma oxidative stress index malonaldehyde (MDA) was decreased significantly after treatment in both groups (P <0.01); the levels of catalase (CAT), superoxide dismutase (SOD) and GSH-Px were increased significantly (P <0.01). The improvement in oxidative stress in observation group was more obvious than that in control group after treatment (P <0.01). The overall score of MLHFQ after therapy was decreased in both groups after treatment (P <0.01); and the MLHFQ score in observation group was significantly lower than that of control group at the same time point after treatment (P <0.01). In addition, there was no statistically significant difference in adverse reactions incidence between observation group and control group. Conclusion: The application of Ziyin Zhuyang Kangshuai decoction combined with western medicine could help to improve TCM syndromes and cardiac function, maintain the balance of body's oxidation and antioxidant system, delay myocardial remodeling and improve the quality of life for patients with CHF. Ziyin Zhuyang Kangshuai decoction is effective and safe, and it brings new direction for clinical prevention and therapy of CHF.
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Fangji Huangqi Tang combined with Zhenwutang for chronic heart failure (Qi deficiency and blood stasis type). Method: According to the inclusion criteria, 80 patients with chronic heart failure (Qi deficiency and blood stasis type) were randomly divided into control group and treatment group (40 cases in each group). The patients in control group received conventional anti-heart failure western medicine (including angiotensin converting enzyme inhibitors or angiotensin Ⅱ receptor blockers, vasodilator drugs, beta blockers, diuretics, digoxin, et al); patients in treatment group received Fangji Huangqi Tang combined with Zhenwutang on the basis of routine treatment, for a total of 4 weeks. Two groups of patients were observed before and after treatment for traditional Chinese medicine (TCM) syndrome curative effect, cardiac function grading, left ventricular end diastolic diameter (LVED), left ventricular end systolic diameter (LVSD), and left ventricular ejection fraction (LVEF). Result: After treatment, the total effective rate for TCM syndrome was 82.5% in treatment group, better than 65% in control group (P <0.05); the total effective rate of cardiac functions grading was 85% in treatment group, better than the 60% in control group (P <0.05); in the index of heart function, LVED and LVSD levels were decreased, and LVEF was increased in both treatment group and control group after treatment (P <0.05); the LVED and LVSD levels in treatment group were lower while LVEF was higher than those in the control group (P <0.01). Conclusion: Fangji Huangqi Tang combined with Zhenwutang has a certain role in improving TCM syndrome, cardiac functions grading, cardiac functions and ventricular remodeling in patients with chronic heart failures.关键词：Fangji Huangqi tang;Zhenwutang;chronic heart failure;cardiac function;clinical observation
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of oral and enema treatment with modified Taohe Chengqitang on early intestinal obstruction after abdominal surgery and its effect on neurological and inflammatory factors. Method: One hundred and thirty-eight patients were randomly divided into control group and observation group by random number table, with 69 cases in each group. Patients in control group got comprehensive therapies including persistent gastrointestinal decompression, rehydration, correction of acid-base balance and water and electrolyte disorder, and anti-infection. And they got ceftazidime for injection, 2 g/time, for 7 days, somatostatin for injection for continuous micro pump pumped, 6 mg/24 hours, for 3 days, and methicillin neostigmine injection, 2 mL/time. intramuscular injection. The observation group in control group to the basis of modified Taohe Chengqitang into stomach and enema, 2 dose/day. The course of treatment was for 7 days. Times of abdominal pain relief, abdominal distension relief, bowel sounds recovery and ventilation defecation, gastric tube retention, eating, body temperature recovery and white blood cell count recovery, length of stay and surgical transit were recorded. Before and after treatment, damp heat stasis syndromes were scored. And levels of vasoactive intestinal peptide (VIP), procalcitonin (PCT), nitric oxide (NO), monocyte chemoattractant protein-1 (MCP-1), serum interleukin-6 (IL-6), tumor necrosis factor -α (TNF-α) and C-reactive protein (CRP) were detected. Result: The total clinical effective rate of traditional Chinese medicine(TCM)in observation group was 91.31%, which was higher than 75.37% in control group (χ2=6.618, P <0.01). Time of abdominal pain relief, abdominal distension relief, bowel sounds recovery, ventilation defecation, gastric tube retention, eating, body temperature recovery and white blood cell count recovery, length of stay and surgical transit were all shorter than those in control group (P <0.01). Transfection rate in observation group was 5.8%, which was lower than 18.84% in control group (χ2=5.434, P <0.05). After treatment, levels of VIP, PCT, NO, MCP-1, IL-6, TNF-α and CRP were lower than those in control group (P <0.01). Conclusion: In addition to western medicine therapy, oral and enema treatment of modified Taohe Chengqitang can regulate nerve mediators, reduce inflammation damage, relieve intestinal obstruction, shorten the course of disease, and improve the success rate of conservative treatment for patients of early intestinal obstruction after abdominal surgery, with a better effect than pure western medicine therapy.关键词：postoperative intestinal obstruction;Taohe Chengqitang;nerve mediator;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Huashi Jiedu decoction on patients infected with high-grade cervical intraepithelial neoplasia (CIN) and high-risk human papillomavirus(HPV) Shire Xiazhu symptom after loop electrosurgical excision procedure(LEEP) operation, and the effect on disease outcome according to the expressions of apoptosis inhibition gene surviving and proliferating cell nuclear antigen Ki67 and the HPV viral load. Method: One hundred and sixty patients with high-grade cervical intraepithelial neoplasia CIN and high-risk HPV were randomly divided into control group (80 cases) and observation group (80 cases) according to random tables generated by SAS software. The two group's patients got LEEP operation. After operation, patients in control group were given LEEP operation recombinant human interferon α-2 b gel outside of cervix. A course of treatment was 10 days, and the treatment shall last for 3 continued menstrual cycles. In addition to the therapy of control group, patients in observation group were also given Huashi Jiedu decoction, 1 dose/day for 3 continued menstrual cycles. And follow-up visit continued for 3 menstrual cycles. Before the operation and at the 6th month after the operation, positive expressions of Survivin, Ki67 and human papilloma virus (HPV) DNA gene (HPV-DNA) virus were detected. At the 3th month before and after the operation, chronic cervicitis and Shirexiazhu symptom were evaluated, and levels of γ-interferon (IFN-γ), and levels of interleukin-2, 4, 10, 12 (IL-2, 4, 10, 12) were detected. Result: The total clinical rate in observation group was 94.52%, which was higher than 81.69% in control group (χ2=6.691, P <0.05). The total rate of traditional Chinese medicine(TCM) syndrome in observation group was 95.89%, which was higher than 78.87% in control group (χ2=9.529, P <0.01). Six months later after the operation, negative rate of human papilloma virus (HPV) was 91.78%, which was higher than 77.76% in control group (χ2=5.699, P <0.05). The hr\|HPV viral load of observation group was lower than that of control group (P <0.01). And positive expressions of Survivin and Ki67 in observation group were lower than that in control group (P <0.05). The negative rate of Survivin in observation group was 73.97%, which was higher than 56.34% in control group (χ2=4.938, P <0.05), the negative rate of Ki67 in observation group was 80.82%, which was higher than 59.15% in control group (χ2=8.067, P <0.01). After 3 months of the operation, levels of IFN-γ, IL-2 and IL-12 in observation group were higher than those in control group, and levels of IL-4 and IL-10 in observation group were lower than those in control group (P <0.01). Conclusion: Huashi Jiedu decoction can improve the clinical efficacy of patients infected with high-grade cervical intraepithelial neoplasia CIN and high-risk HPV, promote the negative conversion rate of hr\|HIV, decrease the rate of viral load and the positive expressions of Survivin and Ki67, regulate the balance of Th1/Th2, so as to prevent CIN from developing to cervical cancer and improve the prognosis.关键词：cervical intraepithelial neoplasia;human papilloma virus;high-risk type;Huashi Jiedu decoction;Survivin gene;proliferating cell nuclear antigen;viral load
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Qihuang Tongluo decoction on uremia peripheral neuropathy (UPN) based on sympathetic skin reaction(SSR), and the mechanism according to uremic toxins and levels of prophylactic factors. Method: One hundred and fifty-four patients with uremia were randomly divided into control group and observation group by random number table. Both groups' patients were given hemodialysis and blood perfusion. Patients in control group got three-dimensional B injection for intramuscular injection, 2 mL/time, 3 times/week, methylcobalamin tablets, 0.5 mg/time, 3 times/day. In addition to the therapty of control group, patients in observation group were also given Qihuang Tongluo decoction, 1 dose/day. One course of treatment was three months. Before and after treatment, Latency (Lat) and peak-pesk amplitude (Amp) were detected by electromyography evoked potentiometer, and sensory nerve conduction velocity of tibial nerve, peroneal nerve and sural nerve were also detected. Before and after treatment, spleen and kidney Qi deficiency and blood stasis syndrome were scored. Before and after treatment, levels of parathyroid hormone (PTH), β2-microglobulin (β2-MG), leptin (LP), homocysteine (Hcy), tumor necrosis factor-α (TNF-α), serum C-reactive protein (CRP), interleukin-6 (IL-6) and interleukin-8 (IL-8) were also detected. Result: The total effect rate of traditional Chinese medicine(TCM) in observation group was 93.06%, which was higher than 74.29% in control group (χ2=9.212, P <0.01). Lat of upper and lower limbs in observation group were shorter than those in control group, and Amp of upper and lower limbs in observation group were longer than those in control group (P <0.01). After treatment, the abnormality rate of SSR in observation group was 26.39%, which was lower than 66.67% in control group before treatment (χ2=23.471, P <0.01), and 44.29% in control group after treatment (χ2=4.983, P <0.01). After treatment, sensory nerve conduction velocity of tibial nerve, common peroneal nerve and sural nerve in observation group were all faster than those in control group (P <0.05 or P <0.01). Levels of PTH, β 2-MG, LP, Hcy, TNF-α, CRP, IL-6, IL-8 were lower than those in control group (P <0.01). Conclusion: Qihuang Tongluo decoction can improve the patient's neurological function, and promote the recovery of peripheral nerve function. Its mechanism may be related to the removal of uremic toxins and the reduction of inflammatory responses.关键词：Uremia;peripheral neuropathy;Qihuang Tongluo decoction;sympathetic skin reaction;uremic toxins;proinflammatory cytokines
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy and compatibility mechanism of Si Junzitang. Method: Chemical components, targets and diseases related to the four traditional Chinese medicine (TCM) herbs were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Compound-target network, target-disease network, protein-protein interaction(PPI) network, and target-pathway network were constructed to explore the mechanism of Si Junzitang. Result: Totally 27 components, 93 targets and 215 diseases related to the four TCM herbs were obtained through screening. The PPI network contains 68 targets, in which key targets involving FOS, ESR1, AR, PPARG and so on. There were 121 gene ontology(GO) entries, including 96 biological process entries, 13 molecular function entries and 12 cellar component entries. Besides, there were 3 KEGG pathways, involving Neuroactive ligand-receptor interaction, calcium signaling pathway and cancer pathway. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Si Junzitang, in order to lay a solid foundation for further studies on the mechanism of action.关键词：Si Junzitang;network pharmacology;target;mechanism
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy and compatibility mechanism of Wuzhuyu Tang. Method: The chemical components and targets related to the four Chinese herbal medicines were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The compound-target network, protein-protein interaction(PPI) network, and target-pathway network were constructed to explore the mechanism of Wuzhuyu Tang. Result: The compound-target network contain 29 chemicalcomponents and 77 targets. The hub targets involve PTGS2, PTGS1 and so on.The PPI network contain 70 proteins, in which the hub proteins involve AP-1, BCL2, HSP90α and so on. In the gene ontology(GO) functional enrichment analysis, there are 145 GO terms (100 Biological Process, 23 Molecular Function, 22 Cellar Component). The pathway enrichment analysis contains 3 pathways:calcium signaling pathway, neuroactive ligand-receptor interaction, and pathways in cancer. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Wuzhuyu Tang, thuslaying a solid foundation for deeply study on the mechanism of action.关键词：Wuzhuyu Tang;network pharmacology;protein-protein interaction (PPI);gene ontology (GO);pathway
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发布时间：2024-01-04 - 摘要：Objective: To investigate the efficacy and mechanism of Shenfutang. Method: The chemical components and corresponding targets related to Shenfutang were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The compound-target network and the protein-protein interaction(PPI) network were established, and the GO functional enrichment analysis and the KEGG pathway enrichment analysis were made to explore the mechanism of Shenfutang. Result: The compound-target network contained 23 chemical components and 118 targets. The hub targets involved PTGS2, PTGS1, NCOA2, ADRB2.The PPI network contained 114 proteins, in which the hub proteins involved AKT, AP-1, p56.The GO functional enrichment analysis showed 236 GO entries (including 191 for biological process, 20 for molecular function, 25 for cellar component). The KEGG pathway enrichment analysis showed 10 pathways, involving calcium signaling pathway, neuroactive ligand-receptor interaction, apoptosis, and pathways in cancer. Conclusion: The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Shenfutang, thus laying a solid foundation for further study on the mechanism of action.关键词：Shenfutang;network pharmacology;protein-protein interaction network (PPI);gene ontdogy (GO);signaling pathway
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of action of Shengmaisan. Method: Potential targets related to 33 active chemical components from Ginseng Radix et Rhizoma,Ophiopogonis Radix and Schisandrae Chinensis Fructus of Shengmaisan were predicted through PharmMapper server. Compound-target network, protein-protein interaction (PPI) network, gene ontology (GO) enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were constructed to explore the mechanism of action of Shengmaisan. Result: Compound-target network contained 249 nodes, in which the key targets involved Transthyretin (TTR), etc. PPI network contained 155 nodes, in which the key targets involved Proto-oncogene tyrosine-protein kinase Src (SRC), RAC-alpha serine/threonine-protein kinase (Akt1), etc. In GO enrichment analysis, there were 95 GO terms, including 67 terms related to biological process, 22 related to molecular function and 6 related to cellar components. In KEGG pathway enrichment analysis, there were 8 KEGG pathways, involving pathways in cancer, prostate cancer, insulin signaling pathway and so on. Conclusion: The results of the study have preliminarily verified the basic pharmacological effects and related mechanisms of Shengmaisan, and laid a solid foundation for further studies on the mechanism of action of Shengmaisan.关键词：Shengmaisan;network pharmacology;protein-protein interaction (PPI);gene ontology (GO);Kyoto encyclopedia of genes and genomes (KEGG)
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发布时间：2024-01-04 - 摘要：Since the medical history has been recorded, tumor is always a major disease which threats human life and health, and how to cure tumor effectively is still one of the most important problems in current medical field. Chinese medicinal formula is the treasure of traditional Chinese medicine (TCM), and it has developed for over two thousand years. Application of Chinese medicinal formula in treating various diseases has a long history under the guide of TCM basic theory. There is no exact word for ‘tumor’ in TCM, but based on the understanding of the pathogenesis of tumor, it can be classified into the category of ‘abnormal mass accumulation’, thus, tumor therapy is an important part of clinical application of Chinese medicinal formula. In the process of tumor treatment, Chinese medicinal formula can fully mobilize the prevention mechanism of human body, play the comprehensive multi-target, multi-level and multi-link regulation effects, and has significant advantages in inhibiting tumor occurrence and development, improving the quality of life and prolonging the survival time of patients. Chinese medicinal formula treatment emphasizes the holistic concept, but because of its variety of chemical compositions, diversity of prescriptions, complexity of pharmacological mechanism and numerous interference factors, the understanding of the interrelation and influence between multi links and multi targets of Chinese medicinal formula is not deep enough. Therefore, the research progress is slow and the international recognition is low. In this paper, the related literature in recent years was collected, collated and summarized, with a researches review on mechanism of anti-tumor effects of Chinese medicinal formula and compound preparations in aspects of inhibiting cell proliferation, affecting cell cycle, inducing differentiation, apoptosis and autophagy, suppressing telomerase activity, tumor invasion and metastasis, controlling angiogenesis and regulating immune status. This paper could provide theoretical basis and scientific basis for prevention and treatment of tumors by TCM.关键词：Chinese medicinal formula;tumor;mechanism;research progress;anti-tumor mechanism
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发布时间：2024-01-04
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