最新刊期
卷 21 ， 期 4 ， 2015
- 摘要：Objective: To explore effects of medicated leaven and enzymes on contents of diester-type alkaloids in fermentation process of parent drug in Huafeng Dan. Method: The α-amylase group,pepsin group,trypsin group,lipase group,mixed enzymes group,original prescription group and negative group were set,HPLC was employed to determine contents of three diester-type alkaloids(aconitine,masaconitine and hypaconitine) from parent drug of Huafeng Dan in seven groups after four weeks in fermentation,gradient elution(0-22 min,20%-23%A;22-40 min,23%-25%A) with mobile phase consisted of [acetonitrile-tetrahydrofuran(25: 15)](A)-0.1 mol · L-1ammonium acetate solution(contained glacial acetic acid 0.5 mL per 1 L)(B) and detection wavelength of 235 nm. Result: After four weeks in fermentation,contents of diester-type alkaloids in all groups were significantly decreased,the content of aconitine was not detected in all groups.Reduction rates of contents of three diester-type alkaloids was between 66.67% and 81.72%,among which the α-amylase group was the lowest.Multiple comparison showed total content of three diester-type alkaloids had significant differences among all groups.Total content of three diester-type alkaloids in the α-amylase group was lower than that in the original prescription group significantly,but it in the pepsin group and the negative group were higher than that in the original prescription group significantly,it in the other six groups was significantly lower than that in the negative group.By comparing with the original prescription group,total content of three diester-type alkaloids in the trypsin group,the lipase group,the mixed enzymes group were no significant difference. Conclusion: Medicated leaven and enzymes in fermentation have some promoting effect on degradation of diester-type alkaloids,α-amylase in promoting effect is significant.关键词：partent drug of Huafeng Dan;medicated leaven;fermentation;diester-type alkaloids;aconitine;mesaconitine;hypaconitine
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发布时间：2024-01-04 - 摘要：Objective: To optimize technological parameters of purification process of total balsamic acids from Benzoinum by anion exchange resin. Method: UV was adopted to determine the content of total balsamic acids with detection wavelength at 223 nm.With adsorption capacity and eluting ratio of total balsamic acids as indexes,three types(717,D211 and D301) of anion exchange resin was seleted by static adsorption-elution test,then technological parameters of purification process was optimized by single factor test. Result: Exchange capacity of 717 anion resin was better than the other two kinds of resins,optimal technological parameters were as follows:the concentration of sample solution 0.02 g · mL-1,pH of 12.0,sample volume of 8 BV,adsorption flow rate of 2 BV · h-1,removed impurities with 3 BV of water and 1 BV of dilute hydrochloric acid,eluted with 5 BV of 95% ethanol,elution flow rate of 1 BV · h-1.Under these conditions,purity of total balsamic acids could reached 73.49%,which increased 2.78 times by comparing with 26.47% before purification. Conclusion: Purification efficiency of 717 anion exchange resin for total balsamic acids from Benzoinum is good,and this optimized purification process is stable and feasible.关键词：anion exchange resin;Benzoinum;total balsamic acids;benzoic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Hyperici Perforati Herba dropping pills. Method: Effects of aqueous extract and alcohol extract of Hyperici Perforati Herba dropping pills on lung index of virus infection in mice were compared by pharmacodynamic trials,which was in order to select extraction method.Taking composite score of extracting amounts of hyperoside,dryocrassin and baicalin as index,effects of ethanol concentration,solvent volume,extracting times and time on extraction process were investigated by orthogonal design.HPLC was employed to determine contents of hyperoside,dryocrassin and baicalin with mobile phase of acetonitrile-0.2% phosphoric acid solution(15: 85) and detection wavelength of 280 nm. Result: Optimum extraction process was refluxing extracted 3 times with 8 times the amont of 70% ethanol for 1.5 h each time.Extracting amounts of hyperoside,dryocrassin and baicalin were 1.50,74.57,167.23 mg · g-1,respectively. Conclusion: This optimized extraction technology is stable,feasible and suitable for industrial production of Hyperici Perforati Herba dropping pills.关键词：Hyperici Perforati Herba dropping pills;lung index;anti-influenza virus;dryocrassin
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发布时间：2024-01-04 - 摘要：Objective: To optimize honey processing technology of Rosae Laevigatae Fructus and establish fingerprint of ethyl acetate extract of this processed product. Method: Taking composite score of contents of total polysaccharides,total flavonoids and extract as index,U7 (76) uniform design was adopted to investigate influences of the content of honey,the amount of dilution water,stir-frying temperature and time on honey processing technology of Rosae Laevigatae Fructus.Fingerprint analysis was employed by 2004B version of 'Chromatographic Fingerprint Similarity Evaluation System of Traditional Chinese Medicine' with mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-20 min,5%-9%A;20-30 min,9%-15%A;30-35 min,15%-17%A;35-60 min,17%-27%A;60-75 min,27%-30%A;75-90 min,30%-40%A) and detection wavelength at 360 nm. Result: Optimum honey processing technology was as following:added 20% honey by comparing with the content of Rosae Laevigatae Fructus,diluted honey by water to 0.4(water compared with honey),heating in 180 ℃ and frying for 6 min.Fingerprints of ten batches of Rosae Laevigatae Fructus had seven common peaks,similarities among ten batches of samples were all>0.8. Conclusion: This optimized honey processing technology is reasonable,liable and stable.Fingerprint analysis method is simple and accurate with good reproducibility,it can be used in quality control of Rosae Laevigatae Fructus processed by honey.关键词：Rosae Laevigatae Fructus;comprehensive evaluation method;honey processing technology;fingerprint;rutin;similarity
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发布时间：2024-01-04 - 摘要：Objective: To investigate feasibility of multi-objective genetic algorithm(MOGA) for optimizing β-cyclodextrin(β-CD) inclusion process of volatile oil from Moslae Herba,thus allowing for subsequent assessment. Method: Taking ratio of β-CD to valatile oil,inclusion temperature and stirring time as factors,with oil content and yield of inclusion compound of volatile oil from Moslae Herba as indexes,β-CD inclusion process of volatile oil was optimized by SGAIAB beta 5008,a plug-in toolbox of Matlab 2009a software,optimization of genetic algorithm entailed statistical analysis by SPSS 19.0 software,whose result was compared with orthogonal test. Result: Optimum inclusion process was:ratio of β-CD to volatile oil 8: 1,inclusion temperature at 59 ℃,inclusion time of 2.8 hours.Following application of MOGA,yield and oil content of inclusion complex were 84.18% and 79.97%,respectively. Conclusion: For multiple targets to be optimized simultaneously,MOGA provides a more reasonable approach for optimizing condition of β-CD inclusion process of volatile oil from Moslae Herba compared with Pareto non-inferior solution and can provide some theoretical basis for preparation of Chinese patent medicines containing Moslae Herba.关键词：Moslae Herba;volatile oil;β-cyclodextrin;orthogonal test;multi-objective genetic algorithm
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发布时间：2024-01-04 - 摘要：Objective: To optimize spray drying process of Stigma Maydis water extract and provide theory support for preparation of Stigma Maydis dispensing granules. Method: According to single factor tests,with composite score of contents of total flavonoids and total polysaccharides,moisture content,flour yield as index,influence of inlet air temperature,air flow and feed rate on spray drying process of Stigma Maydis water extract was investigated by orthogonal test.Contents of total flavonoids and total polysaccharides were determined by UV,detection wavelengths were 400 nm and 490 nm,respectively. Result: Optimum spray drying process was as follows:inlet air temperature of 180 ℃,air flow rate of 800 L · h-1,inlet rate of 20%.Contents of total flavonoids and total polysaccharides,moisture content,flour yield were 0.14%,2.71%,5.81% and 91.33%. Conclusion: This optimized process is rational and stable,it can provide experimental basis for industrial production and product development of Stigma Maydis dispensing granules.关键词：Stigma Maydis;moisture content;spray drying;total flavonoids;total polysaccharides;flour yield
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of compound Changtai granules for industrial production of this preparation. Method: Orthogonal test was employed to investigate influences of the amount of water,extracting time and decoction times on extraction process by taking contents of ginsenoside Rb1,hederagenin and extract as comprehensive evaluation indexes.Contents of ginsenoside Rb1 and hederagenin were determined by HPLC,mobile phases were consisted of acetonitrile(A)-water(B) for gradient elution(0-5 min,10%-30%A;5-20 min,30%-45%A;20-22 min,45%-10%A) and acetonitrile-0.1% aqueous formic acid(70: 30). Result: Optimum extraction technology was:refluxing extracted 3 times with 10,8,8 times the amount of water with 1 h per time.Under these conditions,contents of ginsenoside Rb1 and hederagenin were 2.48,10.50 mg · g-1,respectively;yield of extract was 16.72%. Conclusion: This optimized extraction technology is feasible,stable and suitable for industrial production of compound Changtai granules.关键词：compound Changtai granules;ginsenoside Rb1;hederagenin;yield of extract
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发布时间：2024-01-04 - 摘要：Objective: To optimize separation and purification technology conditions of evodiamine from ethanol extract of Euodiae Fructus by macroporous resin. Method: With adsorption volume and elution rate of evodiamine as indexes,five kinds(AB-8,S-8,NKA-9,HPD-100,D-101) of macroporous resins were selected by static adsorption and desorption test.Sample solution concentration,sample volume,sample flow rate,eluent type,eluent amount and other parameters were investigated by single factor tests. Result: D-101 macroporous resin had the best separation and purification capacity for evodiamine in following technology conditions:sample concentration of 3 g · L-1,sample volume of 4 BV,sample flow rate of 1 BV · h-1,removed water-soluble impurities with 5 BV of water,eluted with 5 BV of 80% ethanol,eluting velocity of 2 BV · h-1.Elution rate of evodiamine was 92.81%,its purity increased from 1.29% to 26.77%. Conclusion: D-101 macroporous resin is suitable for purifying evodiamine from Euodiae Fructus.This optimized method is stable and feasible,it can provide a reference for development and utilization of evodiamine.关键词：Euodiae Fructus;evodiamine;macroporous resin;polar;elution rate;adsorption quantity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents in the aqueous extract of Citri Reticulatae Pericarpium. Method: The chemical constituents were isolated by several chromatographic methods, including silica gel, Sephadex LH-20 and preparative HPLC. Their structures were elucidated by their physical and spectral data. Result: Seven compounds were isolated, including one flavone C-glycoside, two flavanone glycosides, two polymethoxylated flavones, one meliacane and one disaccharide, and elucidated as follows:vicenin-2(1), hesperidin(2), narirutin(3), nobiletin(4), tangeretin(5), limonin(6), maltose(7). Conclusion: Compounds 1 and 7 were isolation from this species for the first time.关键词：Citri Reticulatae Pericarpium;the aqueous extract;chemical constituent;chromatographic method
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发布时间：2024-01-04 - 摘要：Objective: To study chemical constituents of CHCl3extract from Phyllanthus matsumurae and provide the scientific evidence for further development. Method: Various techniques such as silica gel column and Sephadex LH-20 chromatography were used for the isolation and purification. Physicochemical properties and spectroscopic methods including1H-NMR,13C-NMR and MS were used for the identification of structures. Result: Seven compounds were isolated from the CHCl3 extract of 75% alcohol extract of the whole plant and identified to be quercetin (1), 3,3'-dimethylquercetin (2), 3',4'-dimethylquercetin (3), (-)-epigallocatechin (4), 3,3'-O-dimethylellagic acid (5), 3,4-O-dimethylellagic acid (6), and β-daucosterol (7). Conclusion: Compounds 2-7 were reported from this plant for the first time.关键词：Phyllanthus matsumurae;CHCl3 extract;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: In order to make the berberine and splicing of certain chemical composition, needing to form an intermediate largely,through design and experiment, synthesis of 9-O aminoberberine derivatives. Method: With berberine as starting material, through to methyl,bromo, amination reaction, obtain berberrabine, 9-O-(4'-bromobutane) berberine, the final synthesis of 9-O-(4'-butyl amine) berberine, and to determine its relative molecular mass and molecular structure by means of spectroscopic. Result: Through a series of chemicalreactions in organic synthesis are berberrabine and 9-O-(4'-bromo-butane) of berberine, through the optimization of the literature method to prepare 9-O-(4'-butyl amine) berberine derivative, and through the ESI-MS, NMR spectroscopy to determine the structure. Conclusion: By optimizing the method greatly improves the product of 9-O-(4'-butyl amine) berberine yield, and the synthesis process is simple and easy to operate.From the object structure, the terminal amino, can react with various chemical groups, and then introduce the new group, to synthesize more derivatives.关键词：berberine;derivative;butyl amine;synthetise
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发布时间：2024-01-04 - 摘要：Objective: To study the differences of chemical components in raw and processed Atractylodis Rhizoma and to provide a scientific basis and evidence for clarifying the mechanism of processing. Method: Agilent C18column(4.6 mm×250 mm,5 μm)was used with the mobile phase of acetonitrile and water,the flow rate was 1 mL · min-1, the detection wavelength was set at 270 nm. Result: The HPLC fingerprints of raw and processed Atractylodis Rhizoma were established and 18 common peaks were detected,the similarity of 10 batches raw and processed Atractylodis Rhizoma were over 0.9,the peak areas of multi-components including atractylodin,atractylodes lancea and (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate were decreased after processing. Conclusion: HPLC fingerprint has been established to analyze the chemical components in raw and processed Atractylodis Rhizoma,the method is accurate and reliable.关键词：Atractylodis Rhizoma;process;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprints of water-soluble components in Atractylodis Rhizoma and determine the content of atractyloside A. Method: HPLC was adopted to determine the content of atractyloside A with mobile phase of acetonitrile(A)-0.25% aqueous formic acid(B) for gradient elution(0-17 min,2%-5%A;17-25 min,5%-12%A;25-35 min,12%A;35-50 min,12%-17%A;50-70 min,17%-34%A;70-80 min,34%-40%A),detection wavelength at 221 nm and flow rate of 0.6 mL · min-1.The external standard method was employed to determine the content of atractyloside A in ten batches of Atractylodis Rhizoma. Result: Fingerprint of water extract in Atractylodis Rhizoma was established by HPLC,and 18 common peaks were detected.RSD of common peaks relative retention time and relative peak area in precision,stability and repeatability tests were less than 3%.Water extract of ten batches of samples showed high stability with the content of atractyloside A in Atractylodis Rhizoma was 2.775 mg · g-1. Conclusion: This established method is specific,accurate and reproducible,it can be used as a quality control method for Atractylodis Rhizoma.关键词：Atractylodis Rhizoma;water-soluble components;fingerprint;atractyloside A
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发布时间：2024-01-04 - 摘要：Objective: To provide scientific basis for the utilization and development of vines of Mappianthus iodoides by setting up the quality standards. Method: The pharmacognostical methods were applied. The morphological and histological characters of vines of M. iodoides were observed. The extracts, moisture, total ash, and acid-insoluble ash were determined, and the bioactive constituents were analyzed by TLC and HPLC. Result: The quality specification of vines of M. iodoides were as follows:total ash was not more than 8%;acid-insoluble ash was not more than 3%;moisture was not more than 11%, water extracts not less than 4% and alcohol extracts not less than 3%. The qualitative analysis of taraxerone and taraxerol were performed by TLC and quantitative analysis of vanillin and vanillic acid were performed by HPLC, respectively. Conclusion: The established method can be used for the quality control of vines M. iodoides.关键词：vines of Mappianthus iodoides;quality specification;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To rapid determine the content of water-extract in Jingutongxiao pills by near-infrared spectroscopy (NIRS) combined with partial least squares (PLS). Method: The near-infrared spectra was associated with the content of water-extract in Jingutongxiao pills, and the spectral pretreatment method and modeling interval were studied. The quantitative analysis model of water-extract in Jingutongxiao pills was established by combining the NIRS with PLS. Result: The 98 samples of the quantitative analysis model for correlation coefficients (R2), root mean square error of calibration (RMSEC) and root mean square error of predication (RMSEP) were 0.983 26, 0.21 and 0.22, respectively. 18 validation samples of real value and prediction value of the mean relative deviation is 0.09%. Conclusion: It is feasible to rapid determine the content of water-extract in Jingutongxiao pills by NIRS and PLS.关键词：near-infrared spectroscopy;partial least squares;Jingutongxiao pills;water-extract;rapid determination
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发布时间：2024-01-04 - 摘要：Objective: To determine two main active constituents, dihydromyricetin and myricetin, in Tengchasu extracts which are derived from Ampelopsis Grossdentatae Caulis, and to provide scientific basis for the development and utilization of Ampelopsis Grossdentatae Caulis. Method: ODS was used as stationary phase, the mobile phase consisted of methanol and 0.1%H3PO4 water solution, and a gradient elution was used. The detection wavelengths were set at 291 nm for dihydromyricetin and 375 nm for myricetin. Result: The content of dihydromyricetin in the extract from the tender leave was higher than that from the aerial parts, and simple stir-fried can make the content of dihydromyricetin in the extract from the tender leave increased, but as the decocting time extended, the content of dihydromyricetin in the extract decreased. Conclusion: The preparation methods and decocting time have great influences on the content of the active ingredients in tengchasu extracts.关键词：Ampelopsis Grossdentatae Caulis;extract;dihydromyricetin;myricetin;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for simultaneous determination of baicalin, ginsenosides Rg1, ginsenoside Rb1, notoginsenoside R1 and special privet glycosides five in Qibai granules. Method: HPLC analysis was performed on a Prevail C18 column (4.6 mm×250 mm, 5 μm) with methanol(A) -0.2% phosphoric acid (B) as mobile phase by gradient elution [0-5min, 45%A, 5-15 min, 45%-50% A, 15-60 min, 50% A]. The flow rate was 1.0 mL ·min-1, column temperature was maintained at room temperature, and the detection wavelength was set at 203 nm. Result: The linear ranges of baicalin, ginsenosides Rg1, ginsenoside Rb1, notoginsenoside and special privet glycosides were 2.16-21.6, 1.95-19.5, 2.64-26.4, 3.51-35.1 and 1.01-10.1 mg · L-1,respectively. The RSD of precision, reproducibility and stability tests were less than 2%,the average recoveries were ranging from 95.92% to 101.90%. Conclusion: This method is simple, reasonable, reliable and could be used for quality control of Qibai granules.关键词：Qibai granules;HPLC;baicalin;ginsenosides Rg1;ginsenoside Rb1;notoginsenoside R1;special privet glycosides
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发布时间：2024-01-04 - 摘要：Objective: To establish an UPLC-Q-TOF method for the determination of 8 kinds of chemical medicines illegally added in traditional Chinese medicines for relieving cough and asthma. Method: UPLC-Q-TOF and MS mode was adopted to perform the quantitative determination with the Waters Acquity BEH C18 column (2.1 mm ×100 mm, 1.7 μm), methanol-10 mmol ·L-1 ammonium formate (containing 0.1% formic acid) for the gradient elution, and the flow rate was 0.4 mL ·min-1. The ion source was electrospray ionization (ESI) with the positive ion detection. The scan range was m/z 100-600.Theophylline, sulfamethoxazole, chlorphenamine, diphenhydramine, pentoxyverine, benproperine, prednisone acetate and diazepam added in the traditional Chinese medicines were quantitatively identified. Result: The linear ranges of 8 kinds of chemical medicines were 1.251-62.55, 1.043-52.15, 1.057-52.85, 1.002-50.10, 1.147-57.35,1.069-53.45,0.991-49.55,1.111-55.55 mg ·L-1. The average recovery rate of 8 kinds of chemical medicines was 97.8%-103.2%. Conclusion: This method is rapid, simple and accurate, and could be used to detect 8 kinds of chemical medicines added in traditional Chinese medicines for relieving cough and asthma.关键词：UPLC-Q-TOF;relieving cough and asthma;traditional Chinese medicines;illegally added
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发布时间：2024-01-04 - 摘要：Objective: As the long chain hydrocarbons,punicic acid (C18: 3) on the structure of 9(Z), 11(E), 13(Z)-octadecatrienoic acid, which was in 200-400 nm absorption-free and can't be measured directly by UV detector. This experiment adopted column derivatization methods, made the punicic acid with a strong chromophore in maximum absorption in 200-400 nm. The determination method of punicicacid in pomegranate seed oil by HPLC was established in this thesis. Method: The punicic acid were derivatized with ω-bromoacetophenone as derivative reagent, triethanolamine as catalyst, the Diamonsi C18column(4.6 mm×250 mm,5 μm)was used. The detection wavelength was set at 272 nm, column temperature 30 ℃, with methanol-acetonitrile-water(68.5: 20: 11.5)aseluent, the flow rate was 1.0 mL · min-1. Result: The standard curves of punicic acid is linear within in the range of 0.026 6-0.133 0 g · L-1 and the coefficient is 0.999.The average recovery of punicic acid is 98.7%, RSD is 1.8%. Conclusion: The method is reproducible and accurate, which can be used as the determination method of punicic acid inpomegranate seed oil, and used for the quality control of pomegranate seed oil.关键词：pomegranate seed oil;punicic acid;precolumn derivatization;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for simultaneous determination of rehmannioside D, leonuride, acteoside in crude and processed Rehmanniae Radix, then compared the content variation before and after processing. Method: Kromasil 100-5 C18 column (4.6 mm×250 mm,5 μm), the mobile phase was a mixture of acetonitrile (A)-water (B) at a flow rate of 1.0 mL · min-1 for gradient elution, the column temperature was 30 ℃ with analysis was monitored at 203 nm. Result: Three components had good linear relationships:rehmannioside D in the range of 68.10-3 405 ng, leonuride in the range of 99.40-4 970 ng, acteoside in the range of 14.52-726.0 ng, the average recoveries in the range of 97.7%-99.0%. Conclusion: The method was steady with high precision and good sensitivity. It could be used for the quality control of Rehmanniae Radix.
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of exogenous magnesium(Mg) on biomass and contents of effective constituents of Blumea balsamifera in slow growth period of winter. Method: One-year-old B. balsamifera seedlings were applied with MgSO4 · 7H2O which supplied Mg in slow growth period of winter three times.Height,ground diameter,length of leaf,width of leaf and biomass were measured.UV was adopted to determine the content of total flavonoids in different parts of B. balsamifera with detection wavelength of 500 nm.The content of l-bornel in leaves of B. balsamifera were determined by GC,initial temperature was 80 ℃ for 2 min,then warmed to 100 ℃ by 5 ℃ · min-1 at 4 min,and warmed to 200 ℃ by 10 ℃ · min-1 at 10 min.Temperature of injection port was 220 ℃ and FID detector temperature was 240 ℃,injection volume was 0.6 μL and split ratio was 9: 1. Result: Exogenous Mg significantly enhanced growth indexes of this plant,especially of ground diameter,length and width of leaf.As a result,exogenous Mg increased significantly biomasses of leaves,stems and roots,respectively.Biomasses of leaves and stems under 10 g · L-1 Mg treatment were 226.47 g and 140.40 g,which were 10.09 and 5.28 times compared with the control black treatment(CK),respectively.Biomasses of leaves and stems under 15 g · L-1 Mg treatment were 244.88 g and 146.02 g.However,exogenous Mg could not enhance or affect indistinctively the mass fraction of total flavonoids and l-borneol,but it increased qualities of total flavonoids and l-borneol significantly.Qualities of l-borneol under 10,15 g · L-1 Mg treatments were 8.5 and 10.0 times by comparing with CK,respectively. Conclusion: Exogenous Mg can promote plant growth and biomass accumulation,significantly increase qualities of total flavonoids and l-borneol in B. balsamifera in slow growth period of winter.关键词：magnesium;Blumea balsamifera;slow growth period;l-borneol;total flavonoids;biomass
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发布时间：2024-01-04 - 摘要：Objective: To obtain high quality DNA from Curcuma kwangsiensis for simple sequence repeat(SSR) analysis,and provide a reference for research of genetic diversity of this variety. Method: Taking C. kwangsiensis leaves as experimental material,total DNA was extracted by the classic cetyl trimethyl ammonium bromide(CTAB) method and the modified CTAB method,difference between machine grinding and hand grinding was investigated,concentration and purity were determined by UV.DNA as a template for polymerase chain reaction(PCR) amplification,taking clest SSR-01,clest SSR-03,clest SSR-06,clest SSR-08,clest SSR-14 and clest SSR-15 as primers,polyacrylamide gel electrophoresis was adopted to detect DNA amplification effect. Result: When DNA extracted by the modified CTAB method,A260 nm/A280 nm of them was 1.8-2.0,proteins,polysaccharides,RNA and other substances were removed completely,DNA conformed to requirements of PCR amplification result,it could amplify out clear bands by C. universal primers and was suitable for SSR analysis.A260 nm/A280 nm of DNA extracted by the classic CTAB method was 1.5-1.6,it contained some impurities,which cloud not be used for PCR amplification and other molecular biology researches. Conclusion: DNA quality is good which was extracted by the modified CTAB method,this method can effectively remove interferenceof the secondary metabolites for DNA,it is suitable for SSR molecular marker and genetic diversity analysis analysis of C. kwangsiensis.关键词：Curcuma kwangsiensis;genomic DNA;simple sequence repeat;primer;grinding way
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发布时间：2024-01-04 - 摘要：Objective: To detect pharmacokinetics process of quercetin-poly(lactic-co-glycolic acid)(PLGA) block copolymer nanoparticles lyophilized powder in rats,then provide a reference for development and utilization of quercetin preparation. Method: HPLC was employed to determine plasma concentration of quercetin in rats with mobile phase of methanol-4.3% acetic acid solution(75: 25) and detection wavelength at 254 nm,bioequivalence of quercetin-PLGA block copolymer nanoparticles lyophilized powder and intravenons emulsion was compared. Result: Main pharmacokinetic parameters of quercetin-PLGA block copolymer nanoparticles in mice displayed that Cmax 16.129 mg · L-1,t1/2 7.384 h,CL 0.362 L · h-1 · kg-1,AUC0-t 99.461 mg · L-1 · h-1.At the same dose,main pharmacokinetic parameters of the quercetin emulsions group and the quercetin nanoparticles group were highly significant differences.Elimination half-life of the quercetin nanoparticles group was 1.52 times of the quercetin emulsions group.Compared with quercetin emulsions,relative bioavailability of quercetin nanoparticles was 172.92%. Conclusion: Quercetin-PLGA block copolymer nanoparticles significantly change pharmacokinetic behavior of quercetin,drug elimination time slows down,while bioavailability increases.It has a broad application prospects in anti-tumor,especially in treatment of tumor resection of residual disease.关键词：quercetin;block copolymer nanoparticles;poly(lactic-co-glycolic acid);emulsion;pharmacokinetics
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发布时间：2024-01-04 - 摘要：Objective: To study the in situ intestinal absorption kinetics of bajijiasu in rats. Method: The absorption of bajijiasu in the small intestine (duodenum, jejunum and ileum) and colon of rats was investigated using an in situ single-pass perfusion method. The drug concentration was measured by HPLC-ELSD. The effects on intestinal absorption of different drug concentration (43.92, 87.84, 175.68 mg · L-1), different pH in perfusate (5.4, 6.8, 7.4) and P-glycoprotein (P-gp)inhibitor (0, 0.2, 0.5 mmol · L-1) were conducted. Result: Bajijiasu could be absorbed in the whole intestine, and its absorption rate was influenced by the pH of the perfusion solution and intestinal segments. The absorption rate in duodenum, jejunum, ileum and colon was 2.752-2.861, 1.435-1.574, 1.353-1.403, 1.144-1.301 h-1, respecvticvely, the rate declined in turn. Papp in the group containing the P-gp inhibitor increased markedly. Conclusion: The absorption of bajijiasu shows passive diffusion in the general intestinal segments with the first-order kinetic process.关键词：bajijiasu;intestinal absorption;single-pass intestinal perfusion;P-glycoprotein
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发布时间：2024-01-04 - 摘要：Objective: To analyze metabolites of hypaconitine in rats' urine. Method: Urine samples of rats were purified by liquid-liquid extraction,they were detected by liquid chromatography coupled with ion trap mass spectrometry.HPLC was performed by applying gradient elution with mobile phase composed of buffer of 2% ammonia combined with 0.2% acetic acid (A)-methanol blended with acetonitrile of equal volume (B)-methanol(C). Result: Prototype (M1) and three metabolites (M2, M3, M4) of hypaconitine were detected,M2 was identified as 18-O-demethylhypaconitine,M3 was identified as N-demethylhypaconitine,M4 was identified as a hydroxylated metabolite. Conclusion: Hypaconitine may occur demethylation,hydroxylation metabolic reactions,which could provide a reference for further research on pharmacodynamics of hypaconitine.关键词：hypaconitine;metabolites;liquid chromatography-ion trap mass spectrometry;metabolic pathways
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发布时间：2024-01-04 - 摘要：Objective: To study the pharmacokinetic features of microencapsulated canthaxanthin in healthy rabbit. Method: Blood samples were collected from the hind limb vein at different time point after administration of a single dose of microencapsulated canthaxanthin. Plasma canthaxanthin concentration was determined by LC-MS. The main pharmacokinetic parameters were calculated by DAS2.1 program. Result: The concentration-time data of canthaxanthin fitted to a two-compartment model. The main pharmacokinetic parameters were as follows:Tmax=300 min, Cmax=(75.761±0.739) μg · L-1, t1/2z=(527.408±208.401) min, AUC(0-t)=(23 057.221±1 582.299) μg · L-1 · min-1, Vz/F=(1 569.453±258.575) L · kg-1. Conclusion: The microencapsulated canthaxanthin is slowly absorbed, possesses large volume of distribution, and shows stable and sustained-release characteristics in body.关键词：canthaxanthin;microencapsule;pharmacokinetics;rabbit
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect and underlying mechanism of genistein from Hydrococyle sibthorpioides on lipopolysaccharide (LPS)/D-galactosamine (D-GalN) -induced acute hepatic injury in mice. Method: Male mice were randomly divided into normal group, model group, low-, middle-and high-doses of genistein-treated groups. The mice in the normal and model groups were given saline. The animals in the genistein-treated groups were administered intraperitoneally 0.5, 1, 2 mg · kg-1 genistein once daily for 3 days. At the end of treatment, except the mice in the normal group, all mice were intraperitoneally injected with 50 μg · kg-1 LPS plus 800 mg · kg-1 D-GalN. Blood samples were collected from mice eyes 1.5 h after LPS/D-GalN administration, then the mice were killed and liver samples were dissected out after 6 h. The activities of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST) and bilirubin, the contents of hepatic tumor necrosis factor-α(TNF-α), nitric oxide(NO) and malandialdehyde(MDA), as well as the expression of TNF-α, inducible nitric oxide synthase(iNOS), nuclear factor(NF) -κB p65, Caspase-3 and B cell lymphoma/leukemia-2(Bcl-2) were detected. In addition, the damage of liver tissues was observed by light microscope. Result: Compared to the model control, treatment with genistein significantly decreased the AST and ALT activities, further increased the bilirubin level. Histologic examination showed that genistein markedly alleviated the mice livers tissue damage. Furthermore, genistein reduced the pro-inflammatory cytokines including TNF-α and NO/iNOS via inhibiting the NF-κB activity. In addition, genistein inhibited the expression of Caspase-3, enhanced levels of Bcl-2 and total bilirubin. Conclusion: Genistein could prevent LPS/D-GalN-induced acute liver damage in mice, and its underlying mechanisms were mainly due to its ability to block NF-κB signaling pathway for anti-inflammation response and attenuate hepatocellular apoptosis.关键词：genistein;Hydrocotyle sibthorpioides;lipopolysaccharide;D-galactosamine;acute hepatic failure
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of combined therapy of epigallocatechin gallate, taurine, and genistein on CCl4-induced liver fibrosis in rats and its mechanism. Method: The SD rats were divided into six groups randomly:normal group (n=10), model group (n=12), colchicine group (1 mg · kg-1,n=12), combination therapy low [taurine 50 mg · kg-1+epigallocatechin gallate(EGCG) 7.5 mg · kg-1+genistein 5 mg · kg-1, n=12], middle (taurine 100 mg · kg-1+EGCG 15 mg · kg-1+genistein 10 mg · kg-1, n=12) and high (taurine 200 mg · kg-1+EGCG 30 mg · kg-1+genistein 20 mg · kg-1, n=12) dose group. The rats except normal group were induced by intragastric administration (ig) of 50%CCl4 for 14 weeks,and the normal group was given peanut oil. Treatment was started at the 9th week for 6 weeks. Rats were sacrificed after the last administration, and the serum and liver tissue were taken quickly. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), transforming growth factor β1(TGF-β1) and collagen I in serum and total antioxidamt capacity(T-AOC), superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities in liver were measured, and HE staining was performed to evaluate histopathologic changes. Result: Compared with normal group, levels of ALT, AST, TGF-β1 and collagen I increased and liver T-AOC, SOD and GSH-Px activities decreased significantly in the model group (P<0.05). Compared with the model group, levels of ALT, AST, TGF-β1 and collagen I decreased significantly in combined therapy of middle and high dose group, and liver T-AOC, SOD and GSH-Px activities increased significantly in combined therapy of high dose group (P<0.05). Conclusion: Combined therapy has certain curative effect on CCl4-induced liver fibrosis in rats. Its mechanism may involve scavenging free radical, alleviating the lipid peroxidation, inhibiting the expression of TGF-β1 and reducing the synthesis of extracellular matrix.关键词：combination therapy;epigallocatechin gallate;taurine;genistein;liver fibrosis
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of glycyrrhetinic acid on growth and apoptosis of human esophageal carcinoma cell. Method: Eca9706 cells were inoculated in 96-well plates, and 1×104 cells per hole. Cultured for 24 h 100 μL the culture medium containing different concentrations of glycyrrhetinic acid was added respectively, the concentration was 95,114,138,166, 200 mg · L-1. Inhibitive effect of glycyrrhetinic acid on proliferation of esophageal carcinom Eca9706 cells was detected by MTT assay,and cell apoptosis was detected by flow cytometer and confocal laser scanning microscope. Result: In the range of 95-200 mg · L-1,glycyrrhetinic acid with various concentrations effectively inhibited the proliferation of Eca9706 cells in dose dependent manner (P<0.05). Compared with the control group,glycyrrhetinic acid in a concentration of 83,108, 133 mg · L-1 significantly increased the apoptosis rate of Eca9706 cells in 48 h (P<0.01). The confocal laser scanning microscopewas used to observe cell membrane,for control group showed green fluorescence, which may be at the early apoptotic stage;cell membrane indicated green fluorescence and nuclearwith red fluorescence after glycyrrhetinic acid treatment,which was at early and late apoptosis. Conclusion: Glycyrrhetinic acid could significantly inhibit the proliferation of Eca9706 cells through regulating cell apoptosis.关键词：glycyrrhetinic acid;esophageal cancer;Eca9706 cell;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To study the mechanism of Fufang Yuyang powder on the diabetic skin ulcers healing. Method: The SD male rats were given alloxan and skin was scratched to establish the diabetic wound model. The modeling rats were randomly divided into 5 groups:model group, positive control group(Kangfuxin prescription 10.0 g · kg-1, ig), Fufang Yuyang powder group (Fufang Yuyang powder 13.0 g · kg-1,ig), reinforce deficiency group (Buxu prescription 5.1 g ·kg-1,ig), removing blood stasiss group (Quyu prescription 7.9 g · kg-1,ig). enzyme linked immunosorbent assay(ELISA) was used to assay type Ⅰ and Ⅲ collagen, Smad3, Smad7, transforming groowth factor(TGF)-beta at 10, 16, 20 days after modeling. Result: Compared with the model group, Fufang Yuyang powder increased Ⅲ type collagen, Smad7 expression(P<0.05), decreased Smad3 expression(P<0.05), on the 20th day, the action was most significant, Buxu prescription increasedⅠ type collagen expression (P<0.05), increased TGF-β1, Smad3 expression(P<0.05);Quyu prescription increasedⅠ, Ⅲ type collagen expression(P<0.05), increased, Smad7 expression(P<0.05). Kangfuxin group regulated Ⅲ collagen, increased TGF-β1, Smad3 expression(P<0.05). Conclusion: Fufang Yuyang powder can promote the wound healing in diabetes rats, the mechanism may be related to bidirectional regulation of TGF β1-Smad3,Smad7 pathway and adjusting of the proportion of Ⅰ, Ⅲ type collagen in wound tissue.关键词：Fufang Yuyang powder;diabetic wound;collagen;signal transduction
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of dihydromyricetin of Ampelopsis grossedentata on inflammatory factors and secretory phospholipase A2 of group ⅡA (sPLA2-ⅡA) mRNA expression of aortic arch in atherosclerosis rats. Method: Sixty rats were randomly divided into the control group, the model group, the simvastatin group (5 mg · kg-1 ·d-1) and the high-, low-dose dihydromyricetin groups (40,10 mg · kg-1 ·d-1). The atherosclerosis model was induced by feeding a high fat diet and injecting vitamin D3 except rats in the control group. After 24 weeks, the serum C-reactive protein (CRP), tumor necrosis factor (TNF-α), interleukin-1 (IL-1) and sPLA2-ⅡA mRNA expression of the aortic arch were observed. Result: Compared with the normal group, the thickened blood vessel wall, rough endothelial and marked hyperplasia of the smooth muscle fiber were found in the model group. Besides, levels of serum CRP, TNF-α, IL-1 and sPLA2-ⅡA mRNA expression were remarkably increased in the model group (P<0.01). Compared with the model group, the area of vascular lesions and the thickened blood vessel wall were slightly improved in the low-dose dihydromyricetin group. Moreover, the local rough endothelial, smooth endomembrane and mild hyperplasia of the smooth muscle fiber were found in the high-dose dihydromyricetin group. The serum levels of CRP and IL-1 decreased significantly in the high-dose dihydromyricetin group, sPLA2-ⅡA mRNA expression was inhibited in the high-and low-dose dihydromyricetin group (P<0.05). Conclusion: Dihydromyricetin has good therapeutic effect on atherosclerosis rats. Its mechanism may be related to reducing inflammatory factors level and inhibiting sPLA2-ⅡA mRNA expression of aortic arch.关键词：dihydromyricetin;atherosclerosis;inflammatory factors;sPLA2-ⅡA
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shenqi compound recipe on the glucose and lipid metabolism and the protective effect on skeletal muscle of mice with type 2 diabetic macroangiopathy. Method: Selected 60 KKAy mice were randomly divided into the KKAy group, the model group, the Shenqi compound recipe group (14.4 mg · g-1 · d-1) and the rosiglitazone group (1.33 μg · g-1 · d-1) (n=15), another 15 C57BL/6J mice were assigned to the normal group. Spontaneous type 2 diabetic KKAy mice were induced by feeding water containing nitric oxide synthase inhibitor L-arginine methyl ester (L-NAME) and a high fat diet for 8 weeks except for mice in the KKAy group and the normal group. The general conditions, as well as blood sugar levels of animals were monitored. The levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterin (LDL-C) were detected. The morphological changes of the abdominal aorta and skeletal muscle were analyzed. Result: Compared with the normal group, the food intake, the water intake, the urine volume, the weight and the blood sugar increased in the KKAy group, the scratches and wounds were found in the body surface of the model and KKAy groups, the levels of TC, TG, HDL-C and LDL-C raised in the model (P<0.01) and the KKAy (P<0.05) groups. Compared with the model group, the blood sugar, the levels of TC, TG, HDL-C and LDL-C declined significantly in the Shenqi compound recipe group (P<0.05,P<0.01). Moreover, Shenqi compound recipe could improve abdominal aortic intimal edema, improve the skeletal muscle atrophy, edema, fracture, inflammatory state situation when compared with the model group. Conclusion: Shenqi compound recipe could improve the glucose and lipid metabolism, the vascular lesion of mice with type 2 diabetic macroangiopathy, and protect skeletal muscle.
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of different combinations of herbal medicines and foods on weight loss, glucose and lipid-lowering, this is beneficial to humans and would have a broad application value. Method: Fouty-four Kunming mice were divided into the control group and different combination groups [containing different concents of α-amylase inhibitor extracted from red kidney bean, Cassiae Semen, Cratagei Fructus (Crataegus) and Radix et Rhizoma]. The body weight and organ coefficient were evaluated, the lipid, total cholesterol, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin/globulin, total protein (TP), blood sugarg (glucose, GLU) and blood fat were tested after 30-day treatment. Result: Compared with the normal group, the body weight declined, organ coefficient changed (P<0.05, P<0.01), the ALT, AST and TP contents reduced, albumin/globulin values raised, GLU level reduced, high-density lipoprotein raised, total cholesterol and triglyceride declined in all combination groups (P<0.05, P<0.01). Different effects on low density lipoprotein were obtained from the different combination groups (P<0.05, P<0.01). Conclusion: The experiment results showed that the α-amylase inhibitor played a significant role in reducing body weight, glucose and lipid-lowering when combined with cassia seed, hawthorn and red sage root.关键词：α-amylase inhibitor of kidney bean;Cassiae Semen;Cratagei Fructus;Solviae Miltionhizae Rodix et Rhizoma;reducing body weight;glucose and lipid lowering
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Lianxia formula granule (LXFG) on left ventricular remodeling and cardiac function of rats after acute myocardial infarction (AMI). Method: Ninty healthy adult male Wistar rats were randomly assigned to six groups:the sham operated group, the model group, the metoprolol group, the low-, moderate- and high-dose LXFG groups (47.25, 94.50, 189.00 mg · kg-1). The AMI rats were induced by the ligation of the left anterior descending coronary artery except for rats in the sham operated group. The rats in the sham operated group and model group were given with normal saline, and the other rats received the corresponding medicines by intragastric administration for 30 days. The left ventricular ejection fractions (EF), factional shortening (FS), left ventricular mass (LV Mass), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic dimension (LVEDD), left ventricular end-systolic dimension (LVESD), end diastolic left ventricular posteriol wall thickness (LVPWd) and the end systolic left ventricular posterior wall thickness (LVPWs) of each rat were detected by echocardiogram. Result: Compared with the the model group, EF and FS of LXFG group at 189.00 mg · kg-1 dose enhanced (P<0.05),and LVEDV, LVESV, LVEDD, LVESD, LV Mass, LVPWd and LVPWs of LXFG group at 189.00 mg · kg-1 dose decreased (P<0.05), LV Mass and LVPWs of LXFG group at 47.25 mg · kg-1 dose decreased (P<0.05, P<0.01). Compared with the metoprolol group, LV Mass of LXFG group at 189.00 mg · kg-1 dose decreased (P<0.05). Conclusion: Application of LXFG could improve left ventricular remodeling and left ventricular function in rats after AMI.关键词：Lianxia formula granule;acute myocardial infarction;ventricular remodeling;left ventricular function
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect of total flavonoids of Clerodendranthus spicatus on Parkinson disease (PD) in rat and cell models. Method: The PD model was induced by positioning injection of 6-hydroxydopamine on right substantia nigra of rats. Seventy PD rats were randomly divided into 5 groups:the model group;the high-, middle-, low-dose total flavonoids of C. spicatus groups (45, 22, 11 mg · kg-1 · d-1);the madopar group (7.8 mg · kg-1 · d-1). Another 10 healthy rats were assigned to the control group. After 14 days of treatment, the levels of malondialdehyde (MDA), catalase (CAT), glutathion peroxidase (GSH-Px), superoxide dismutase (SOD), dopamine (DA) and 4-hydroxy-3-methoxyphenylacetic (HVA) in brain tissues of rats were detected by ELISA. SH-SY5Y cells were divided into the control group, the model group, and different dosage of total flavonoids of C. spicatus groups (2, 1, 0.5, 0.25 g · L-1). The PD cell model was induced by adding 30 μmol ·L-1 6-OHDA. The survival rate and morphology were observed and compared after 20-h incubation. Result: Compared with the control group, rotating frequency of rats in PD model group increased, the levels of CAT, GSH-Px, SOD and HVA in brain tissue decreased significantly, MDA level increased with statistically significant difference (P<0.01). Compared with the model group, rotated times of PD rats decreased significantly in the high-dose total flavonoids of C. spicatus group (P<0.05), the levels of CAT, GSH-Px, SOD and HVA increased, MDA concentration decreased in all doses of total flavonoids of C. spicatus groups (P<0.05, P<0.01). However, the improvement of neurological behavior and DA level in the total flavonoids of C. spicatus groups were not as good as madopar. Moreover, the total flavonoids of C. spicatus(2, 1 g · L-1) pretreatment could reduce the cellular damage caused by 6-OHDA as compared with the control group(P<0.05,P<0.01). Conclusion: Total flavonoids of C. spicatus have obvious protective effect on the PD rat model and cell model induced by 6-OHDA. The protective effects may be related to reducing cell damage by resisting oxidative stress.关键词：total flavonoids of Clerodendranthus spicatus;dopamine;parkinson disease;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To identify the anti-inflammatory active fraction from fruits of Capparis spinosa preliminarily of Uighur medicine. Method: Fruits of Capparis spinosa were extracted by distilled water, 80% ethanol and petroleum ether to obtain the different polar fractions groups. Every polar extractions was divided into high, middle, low groups, respectively. The tripterygium glycosides tablet was selected as a positive control drug. The anti-inflammatory active fraction was screened through auricle edema induced by xylene and paw edema induced by carrageen in mice. After 14 days of treatment, the degree of auricle edema and paw edema were measured, the prostaglandin E2 (PGE2) of ankle was detected. Result: The results suggested the foot swelling degree was inhibited, the PGE2 content decreased in the tripterygium glycosides tablet and all extracts groups in xylene-induced auricle edema mice (P <0.05). The paw edema degree was inhibited in the tripterygium glycosides tablet and all extracts groups in carrageen-induced paw edema mice (P <0.05). The distilled water extracts at all doses from Capers fruits had the best results on acute inflammatory reactions than the other groups(P <0.05). Conclusion: Distilled water extracts from Capers fruits have potential anti-inflammation effect. The distilled water extracts may be the anti-inflammatory active fraction from fruits of C. spinosa.关键词：Capparis spinosa;anti-inflammation active;extract
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Bushen Guben decoction (BGD) on bone mineral density (BMD), bone mineral content and serum calcitonin (CT) level in an ovariectomized rat model of osteoporosis. Method: One hundred and five female Wistar rats were randomly divided into the control group, the model group, the Xianlin Gubao group(500 mg · kg-1), the estradiol valerate group (90 μg · kg-1)and high-, medium-, low-dose BGD groups (16,8,4 g · kg-1). The rats except the control group were subjected to bilateral ovariectomy and killed after 3-month treatment. BMD and bone mineral content in the femur and lumbar vertebra, their biomechanical properties were measured by dual-energy X-ray absorptiometry measurement instrument and X-ray. The serum CT level was detected by ELISA method. Result: Compared with the normal group, the BMD and bone mineral content decreased significantly (P<0.05), elasticity modulus, maximum loads and yield strength of femur and lumbar vertebra reduced (P<0.05), serum CT level declined (P<0.01) in the model group. Compared with the model group, the BMD and bone mineral content increased in all treatment groups (P<0.05, P<0.01), the biomechanical properties had good improvement in Xiaoling Gubao group and high-, medium-dose BGD groups (P<0.05, P<0.01), serum CT level raised in high-, medium-, low-dose BGD groups (P<0.05, P<0.01). High-dose BGD had better results in the elasticity modulus of lumbar vertebra and CT level than other groups. Conclusion: BGD had good improvement on osteoporosis in ovariectomized rats by affecting BMD and bone mineral content, CT level and biomechanical properties.关键词：Bushen Guben decoction;osteoporosis;bone mineral density;bone mineral;calcitonin level
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Arctii Fructus on the content of testosterone, correlated hormones and anti-fatigue ability of rats after exercise. Method: By using the model of high-intensity endurance training, fifty-five 6-week-old male Wistar rats were randomly divided into 5 groups, with 10 in each group (5 rats which did not meet the requirement were removed):still in control group (C group), motion control group (M group), exercise+ig low-dose Arctii Fructus group (AML group), exercise+ig middle-dose Arctii Fructus group (AMM group), and exercise+ig high-dose Arctii Fructus group (AMH group). Gavage was performed using professional device once a day. The rats in Arctii Fructus groups were gavaged with 0.5, 1, 3 g · kg-1 with ig volume of 5 mL · kg-1. The rats in C and M groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: Body weight of the rats in M groups lower than that in C group (P<0.05), and that in all doses of Arctii Fructus groups was higher than in M group (P<0.05) which did not show any differences between groups. Swimming time in all doses of Arctii Fructus groups was longer than in M group (P<0.01), and there were dose-response. But there were no differences between C and M groups. Serum testosterone in M group was lower than C group (P<0.01), in the same time, serum testosterone was higher in all doses of Arctii Fructus groups than M group (P<0.01). The serum corticosterone levels in each group showed no significant differences. Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. There were no differences in luteinizing hormone and follicle-stimulating hormone between C group and M group. But luteinizing hormone in all doses of Arctii Fructus groupswas higher than in M group (P<0.05). In the mean time, follicle-stimulating hormone in all doses of Arctii Fructus groups was higher than in M group (P<0.05). There were no differences in both luteinizing hormone and follicle-stimulating hormone between groups in all doses of Arctii Fructus groups and there were dose-response. Conclusion: The supplement of Arctii Fructus can alleviate the hypothalamus-pituitary-gonad axis function disorder caused by exercise through multiple targets and multiple ways.Arctii Fructus can prevent the decrease of serum testosterone after high-intensity exercise and improve anti-fatigue ability.关键词：Arctii Fructus;testosterone;corticosterone;luteinizing hormone;follicle-stimulating hormone;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: To observe growth inhibition of Huangqi injections on human immortalized cervical epithelial cells(H8 cells) and make research on cell cycle regulation mechanism. Method: Effect of Huangqi injections on H8 cells was detected by MTT,form of cells after being injected was observed on optical microscope,cells cycle of H8 was analyzed by flow cytometry(FCM).First antibodies of immunofluorescence assay were cyclins D1 and B1,cyclin-dependent kinases(CDK) 1 and 4,cyclin-dependent kinase inhibitor(p21),second antibody was fluorescein labeled,fluorescence degree of H8 cells after being injected Huangqi injections was determined by FCM. Result: There was inhibiting effect on growth of H8 cells after being injected,function was the most obvious of 20 g · L-1.Some of the Huangqi injections treatment group appeared to be sparse in cell growth,death or apoptosis,cells stained pyknosis hyperchromatic circular corpuscle.When Huangqi injections on H8 cells,S and G2 phase cells decreased,cell blocked in Gl period.Expression of cyelin D1 protein in H8 cells significantly decreased;expression of cyclin B1,CDK4 and P21 were slightly reduced,but without statistical significance;expression of CDK1 protein reduced significantly. Conclusion: Huangqi injections can be regarded as a cell cycle regulation agent for prevention or adjuvant therapy of cervical cancer.关键词：Huangqi injections;cervical immortalized;cell cycle;expression of protein;cell morphology
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发布时间：2024-01-04 - 摘要：Objective: To observe the impact of Saposhnikoviae Radix of Tongxie Yaofang decoction on peroxisome proliferator-activated receptor (PPAR-γ) protein expression in the ulcerative colitis rats, and to explore the role of Saposhnikoviae Radix. Method: Sixty female SD rats were randomly divided into 6 groups, 10 rats in each group. The rat ulcerative colitis(UC) model was made with 50% 2,4,6-trinitrobenzenesulfonic acid(TNBS)ethanol solution.The corresponding prescription without and with Saposhnikoviae Radix,half dose of Saposhnikoviae Radix were orally given, the dose was 18,20,22 g · kg-1, positive group was given salicylazosulfapyridine (SASP) at dose of 0.3 g · kg-1. The weight change, the colon mucosa damage index and colon pathology were observed, and immunohistochemical detection of PPAR-γ protein expression level was carried out. Result: The model group rats had colon mucosa inflammation and ulcer formation, compared with the blank group the result was statistically significant (P<0.05). Expression of PPAR-γ protein in model group rats colon tissue was lower than that in the control group, the difference was statistically significant (P<0.05).After the treatment, PPAR-γ protein expression level in Tongxie Yaofang decoction group was significantly higher than that in groups without or reduced Saposhnikoviae Radix (P<0.05). Conclusion: Saposhnikoviae Radix is minister drug in Tongxie Yaofang decoction, its dosage has significant impact on action of the decoction.关键词：ulcerative colitis;Tongxie Yaofang decoction;Saposhnikoviae Radix;minister drug
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of flavonoids from Psidium guajava leaves on the hepatic glucose metabolism and insulin signaling pathway in streptozotocin (STZ) induced diabetic mice. Method: Male BALB/c mice were injected with STZ (60 mg · kg-1, ip) for 5 consecutive days to generate diabetic model, which was indicated by fasting blood glucose concentration ≥11.1 mmol · L-1 for 3 consecutive days. Diabetic mice were randomly divided into model group and P. guajava leaves flavonoids low, medium and high-dose treatment group,every group has 10 mice, the normal control with 10 mice was set.Flavonoids from P. guajava leaves was administered intragastrically at 0.093,0.186,0.372 g · kg-1 · d-1. After 2 weeks of administration, the fasting blood glucose level and fasting weight were measured. In addition, the mice were euthanized and the number of islets, pathological changes were observed. Real time fluorescence PCR was used to investigate the mRNA expression of hepatic glucokinase(GK), glucokinase regulatory protein(GKRP), glucose transporter protein 2(GLUT2),insulin growth factor (IGF-1), insulin receptor substrate-1(IRS-1) and insulin receptor substrate-2(IRS-2). Result: Compared with the diabetic mice, the blood glucose level decreased (P<0.05), the numbers of islets, the hepatic mRNA expression of GK, GLUT2, IGF-1, IRS-1 and IRS-1 were increased significantly increased (P<0.01) and the hepatic mRNA expression of GKRP in P. guajava leaves flavonoids groups was decreased (P<0.01),the medium dose group showed the best action(P<0.05). Conclusion: Flavonoids from P. guajava leaves has the hypoglycemic effect, and the metabolism is likely through the regulation of hepatic glucose metabolic moleculars and insulin signaling pathway, and also the islet regenerating effects.关键词：flavonoids of Psidium guajava;diabetes;liver;glucose metabolism
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of Fuling prescription(FLP) on anti-senility of Caenorhabditis elegans. Method: C. elegans were divided into the control group and high dose,medium dose and low dose groups of FLP.Effect of FLP on lifespan of C. elegans was observed by life test,effect of FLP on fertility of C. elegans was detected,acute heat stress and oxidative stress were conducted to test ability of stress-resistance and to explore mechanism of FLP. Result: Average lifes of each group were (19.96±1.48),(36.03±1.76),(28.47±1.01),(19.39±0.61) d,maximum lifespans were (21.01±1.36),(37.71±2.38),(29.19±3.12),(22.00±2.01) d.Compared with the control group,FLP could significantly extend average life,maximum lifespan and lifespan in acute heat stress and oxidative stress environment of C. elegans.But there wAs no significant difference of total subalgebra between the control group and FLP groups. Conclusion: FLP has a remarkable effect of anti-senility,its mechanism may have relation to improvement of stress resistance ability of C. elegans.关键词：Fuling prescription;Caenorhabditis elegans;lifespan;fertility;heat stress;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To observe the acutea hepatotoxicity and nephrotoxicity of geniposide on α-naphthylisothiocyanate (ANIT) -induced jaundice rats, and to provide scientific basis for the clinical application of gardenia. Method: One hundred and ninety rats were divided into three following groups:the normal group, the model group and the geniposide group. The alanine aminotransferase (ALT), aspartic transaminase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL), blood urea nitrogen (BUN) and creatinine (CREA) activity in serum were detected at different time. The morphological changes of liver and kidney tissue were observed under light microscope. Result: Compared with the normal group, the levels of AST, ALP, ALT, TBIL, BUN and CREA increased in the model and geniposide groups. The results in the geniposide group were even worse for AST, ALP and ALT at 48, 72 h, for BUN and CREA at 24, 48, 72 h than the model group. Under light microscope, serious pathological damage in liver and kidney tissue of rats were observed in the model and geniposide groups, which were more serious at 48, 72 h. Conclusion: Geniposide could cause acute liver and kidney injury on jaundice rats.关键词：gniposide;jaundice animal models;hepatotoxicity;nephrotoxicity
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of Shixiang plaster on treating chronic ulcer by randomized multicenter research. Method: One hundred and fifty-five patients with chronic ulcer from multicenter were randomly divided into the experimental group and the control group. Eighty patients in the experimental group were treated with Shixiang plaster, and the others in the control group were treated with Kangfuxin liquid. The wound healing rate, healing time, and visual analogue scale were recorded. Result: The wound healing rate showed statistical differences between two groups after 14 days treatment. There were no statistical differences in visual analogue scale between the two groups before treatment, while the visual analogue scale decreased more in the experimental group after treatment than the control group. The healing time in,the experimental group were much lower than the control group. The total curative effect in the experimental group was better than the control group. Conclusion: Shixiang plaster could improve the healing of the wound, which is worthy of clinical promotion.关键词：Shixiang plaster;chronic ulcer;draining pus and eliminating slough;pus-retaining and muscle-generating
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Bailong Lingsha Tang (BLLST) on cellular immune function in the treatment of advanced non-small cell lung cancer. Method: Totally 60 patients with advanced non-small cell lung cancer were randomly divided into the control group and the treatment group of 30 patients each. Patients in the control group were treated by routine chemotherapy, while patients in the treatment group were treated by combination of BLLST and routine chemotherapy. Changes of KPS and symptom scores were observed in both groups. Result: Compared with the control group, percentage levels of CD3+, CD4+,CD4+／CD8+, and NK cells increased, percentage levels of CD8+, CD19+decreased in the treatment group with significant difference (P<0.05) after 2-cycle chemotherapy. Improvement of symptoms in the treatment group (86.70%) was higher than that in the control group (56.70%) with significant difference (P<0.05). Improvement of KPS, quality of life in the treated group after treatment was superior to those in the control group with significant difference (P<0.05). Conclusion: Combination of BLLST and chemotherapy could increase the cellular immune function, improve and relax lesions symptoms, and improve the quality of life.关键词：advanced non-small cell lung cancer;Bailong Lingsha Tang;cellular immune function;chemotherapy
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Haima Bushen Zhuangyang pill on sperm concentration, activity ratio and linear velocity of idiopathic asthenospermia. Method: One hundred and forty patients were divided into the control group and the treatment group according to SAS 8.0 randomization procedure. Finally, 66 patients in the treatment group and 62 patients in the control group were enrolled. The patients in the treatment received self-made Chinese medicine of Haima Bushen Zhuangyang pill and the patients in the control group received Yougui pill. All patients received 60 days of treatment. Result: The total efficiency of the treatment group was 86.36%, which was better than that of the control group (P<0.05). After treatment, concentration of forward movement sperm was (18.60±10.45)×106/mL, activity ratio was (33.78±16.22)%, total activity rate of sperm was (50.43±19.93)%, and linear velocity was (46.88±15.93) μm · s-1 in the treatment group, which was better than the control group (P<0.05). Conclusion: Haima Bushen Zhuangyang pill could improve the main clinical indicators of idiopathic asthenospermia effectively.关键词：Haima Bushen Zhuangyang pill;idiopathic asthenospermia;clinical curative effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Huoxue Ditan decoction on the recovery of neural function and to investigate its mechanism of action in treating hypertensive cerebral hemorrhage disease (HICH). Method: Ninety-four patients with HICH were randomly divided into the control group (47 cases) and the observation group (47 cases) by a random number table. Patients in the control group received Western medicine comprehensive treatment including surgical treatment, dehydration of intracranial pressure, blood pressure control, prevent infection, nerve nutrition and expectant support. Based on the treatment of the control group, patients in the observation group added one does Huoxue Ditan decoction once daily. All patients received 14 days of treatment. The national institutes of health USA nerve function defect (NIHSS) scores and cerebral edema were evaluated before treatment, at day 7,14.Levels of complement C3, C4, hypersensitive c-reactive protein (hs-CRP), neuropeptide Y (NPY) and serum neuron specific enolization enzyme (NSE) were tested. Result: The clinical curative effect of the observation group was superior to that in the control group (P<0.05). Levels of NPY, NSE and hs-CRP in the observation group were less than those of control group (P<0.01). Compared with before, levels of C3, C4 increased in the control group after treatment (P<0.01). However, changes in the observation group were not obvious. Levels in C3, C4 in the observation group were lower than those in the control group (P<0.01). NIHSS score in the observation group was lower than that in the control group at day 7, 14 (P<0.01). Cerebral edema volume in the observation group decreased compared with the data before and seven days after treatment (P<0.01). Ccompared with before and day 7, volume of cerebral edema at day 14 in the observation group went down (P<0.01). Edema volume at day 7, 14 in the observation group decreased persistently (P<0.01), and they were lower than those in control group (P<0.01). Conclusion: Based on the conventional Western medicine comprehensive therapy, Huoxue Ditan decoction could receive edema and improve the recovery of neurological function. Its mechanism of action may be related to reducing the vascular resistance, the inflammatory response and the nerve injury caused by the complement system.关键词：hypertensive cerebral hemorrhage;Huoxue Ditan decoction;neuron specific enolization enzyme;hypersensitive c-reactive protein;neuropeptide Y
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发布时间：2024-01-04 - 摘要：Objective: To discuss the influence of Tongluo Huazhuo decoction on carotid intima-media thickness (IMT), serum inflammatory factors and endothelial function in treating diabetes disease. Method: Ninety-six patients with carotid artery IMT of type 2 diabetes were randomly divided into the control group (48 cases) and the observation group (48 cases) by a random number table. Patients in the control group received 20 mg atorvastatin calcium tablets at night once daily. Based on the treatment of the control group, patients in the observation group added 1 dose Tongluo Huazhuo decoction once daily. All patients in two groups received 4 months of treatment. Before and after the treatment, carotid IMT and artery plaque area were detected, and levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein-C (HDL-C) and low density lipoprotein-C (LDL-C) were also detected. Besides, fasting blood glucose (FBG), fasting insulin (FINS) and glycosylated hemoglobin (HbAlc) were detected and homeostasis model assessment of insulin resistance (HOMA-IR) was calculated, while levels of tumour necrosises factor-α (TNF-α), high-sensitivity C-reactive protein (hs-CRP), adiponectin (APN), homocysteine (HCY) and von Willebrand factor (vWF) were detected. Result: The carotid IMT and artery plaques area in two groups declined compared with the data before treatment (P<0.01). The carotid IMT and artery plaques area in the observation group were less than those in the control group (P<0.01). TC level in the observation group was lower, while the HDL-C level was higher than that in the control group (P<0.05). FBG, HbA1c, FINS and HOMA-IR of both groups decreased after treatment (P<0.01). There was no statistically significant difference between two groups. TNF-α, hs-CRP, Hcy and vWF levels in the observation group were lower than those in the control group (P<0.01). Besides, the APN level was lower than that in the control group (P<0.01). Conclusion: Tongluo Huazhuo decoction could reduce the carotid IMT and artery plaques area. Its mechanism may be related to adjusting lipid metabolism, reducing inflammation and improving endothelial function in type 2 diabetes patients.关键词：type 2 diabetes;carotid intima-media thickness;Tongluo Huazhuo decoction;inflammatory response;endothelial function
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发布时间：2024-01-04 - 摘要：Objective: To discuss the curative efficacy of Tiaochong Xiaozheng decoction in treating hyperplasia of mammary glands and to investigate its influence on endocrinology and endothelin. Method: One hundred and twenty-six patients with hyperplasia of mammary glands were randomly divided into the control group (63 cases) and the observation group (63 cases) by a random number table. Patients in the control group received 4 grains Rubi Sanjie capsules thrice daily. Patients in the observation group received Tiaochong 1 dose Xiaozheng decoction once daily. All patients in two groups received 12 weeks of treatment. Before and after treatment, scores of pain and mass were graded. Contents of estrogen (E2), prolactin (PRL), progesterone (P), testosterone (T), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were detected. Molybdenum target X-ray was detected, and relapse constitution of patients who had curative efficacy was recorded during the 6-month follow-up. Result: The curative efficacy in the observation group was superior to that in the observation group (P<0.05). X-ray molybdenum target photography effect in the observation group was better than that in the control group by ridit analysis (P<0.05). Scores of breast pain, breast tenderness, lump hardness, mass distribution and lump size in the observation group were less than those in the control group after therapy (P<0.01). Levels of E2 and PRL in the observation group were lower than those in the control group (P<0.01), while the P level was higher than that in the control group (P<0.01). After treatment, serum VEGF and bFGF levels in the observation group were lower to those in the control group (P<0.01). The recurrence rate in the observation group was 27.12%, which was less than 47.06% in the control group (P<0.05). Conclusion: Tiaochong Xiaozheng decoction could reduce pain feeling, soften and narrow the mass, and reduce the recurrence rate with mammary gland hyperplasia disease. Its clinical curative effect is obviously distinct. Its mechanism may be related to regulating endocrine and inhibiting angiogenesis factor.关键词：mammary gland hyperplasia disease;Tiaochong Xiaozheng decoction;endocrine;vascular endothelial growth factor;basic fibroblast growth factor
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effect of Xiaoyu Kuanxiong decoction combined with closed drainage of the pleural cavity in treating traumatic hemothorax and to investigate its influence on patients' blood coagulation and hemorheology. Method: Seventy-six patients were randomly divided into the control group (38 cases) and the observation group (38 cases) by a random number table. Patients in the control group received combined treatment, mainly closed drainage of pleural cavity therapy. Based on the treatment of the control group, patients in the observation group added 1 dose Xiaoyu Kuanxiong decoction once daily. All patients in two groups received 12 weeks of treatment. The volume and duration of drainage, and the length of hospital stays were recorded. The occurrence rates of coagulated hemothorax and fibrothorax were observed during the follow-up. Before and after one week of treatment, prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and D-dimer (D-D) in plasma were detected, and the hemorheology indexes, levels of thromboxane B2(TXB2) and 6-ketone-prostaglandin F1α(6-Keto-PGF1α) were also detected. Result: The clinical curative effect of the observation group was superior to that of control group. There was statistically significant difference between two groups (P<0.05). The volume and duration of drainage, and the length of hospital stays in the observation group were less than those of the control group (P<0.05). The incidence of coagulated hemothorax or fibrothorax in the control group was 18.42%, which was higher than 2.63% in the observation group, but there was no statistically significant difference between two groups. After a week of treatment, the levels of D-D and FIB in the observation group were lower than those in the control group (P<0.01). Improvement of blood rheology indexes (whole blood viscosity, plasma viscosity, erythrocyte sedimentation rate, platelet aggregation rate, whole blood reduced viscosity) in the observation group were superior to those in the control group (P<0.01). TXB2level of the observation group was less than that of control group (P<0.01), while the 6-Keto-PGF1α level in the observation group was higher than that in the control group (P<0.01). Conclusion: Xiaoyu Kuanxiong decoction could reduce drainage of traumatic hemothorax patients, shorten the distance of disease, improve blood circulation and promote the blood running smoothly. Besides, it could reduce the incidence of coagulated hemothorax or fibrothorax.关键词：hemothorax;Xiaoyu Kuanxiong decoction;blood coagulation function;hemorheology
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effectiveness and safety of Tianma Gouteng Decoction (TGD) combined with angiotensin-converting enzyme inhibitors (ACEI) in treating patients with primary hypertension. Method: Systematic literature searches were performed in Chinese biomedical, China National Knowledge Infrastructure (CNKI) and Wanfang database. Randomised control trials (RCT) about TGD combined with ACEI in treating patients with primary hypertension were enrolled. The quality of the RCT were assessed using Jadad scores. The effectiveness (clinical effect, blood pressure) and safety were evaluated using meta-analysis. Result: A total of 18 articles were included. Only one article scored 3 according to the Jadad, and the others scored less than 3.Compared with ACEI, TGD combined with ACEI had significant higher clinical efficacy [RR=1.18, 95% CI (1.12, 1.24)]. After treatment, weighted mean difference of systolic blood pressure and diastolic blood pressure were [-4.78, 95%CI (-8.35, -1.21)], and [-4.32 95%CI(-6.34,-2.30)], respectively. TGD combined with ACEI did not increase the risks for side effect. Conclusion: TGD combined with ACEI has good effectiveness, and there was no increase in the side effect. Therefore, it is recommended in clinical practice. However, it is necessary to carry out high-quality double-blinded RCT with large sample size.关键词：Tianma Gouteng decoction;hypertension;angiotensin-converting enzyme inhibitors;Meta-analysis
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发布时间：2024-01-04 - 摘要：To analyze the chemical constituents and antitumor effects of the root of Actinidia chinensis, in order to provide scientific and theoretical basis for investigating its pharmacodynamic substances. The chemical constituents and antitumor effects were reviewed from related domestic and abroad published literatures in the past 30 years. The chemical constituents of the root of A. chinensis are complex. There are about 90 chemical constituents isolated and identified up to now, including 38 pentacyclic triterpenes, 16 flavonoids, 6 anthraquinones, 5 steroids, 5 alkaloids and 25 other type of compounds. It has significant antitumor activities on gastric cancer, esophageal cancer, colon cancer and lung cancer. Herein, we obtained some progress on its chemical constituents and pharmacological activities, especially its antitumor effects. However, limited research has focused on its aqueous extract. Further research is helpful for the development of new medicines and making the most use of plant resources.关键词：root of Actinidia chinensis;chemical component;antitumor effects;pharmacological action
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发布时间：2024-01-04 - 摘要：Lipid is a key cell signaling molecule. It is also involved in the regulation of many important life activities, including material transport, structural support, energy conversion, cell growth, differentiation and apoptosis. As a subdiscipline of metabonomics, lipidomics is an important approach to comprehensive analysis of lipids metabolism. Through analyzing the lipid profiles under different physiological conditions, lipidomics research can indicate the changes of the lipid metabolic network and obtain the lipid-based biomarkers contributed to the changes. Many studies have demonstrated that abnormal metabolism of lipids is closely related to the development of respiratory system diseases. Using lipidomics technique can reveal the changes of respiratory system diseases-associated lipids, find out the abnormal metabolic pathways and identify the biomarkers finally. Thus, lipidomics have shown great potential applications in the early diagnosis and development of disease, as well as the discovery of drug targets. The paper summarized and analyzed the progress and prospects of structured lipids and signal lipids in bronchial pneumonia, asthma, acute lung injury, pulmonary fibrosis and other respiratory diseases in recent years. We focus on the structure lipid including phosphatidylcholine, phosphatidylglycerol, sphingolipids, and signaling lipids including eicosanoids (such as prostaglandins), thromboxanes, leukotrienes, lipoxins, hydroxyeicosatetraenoic acids, isoprostanes and so on. Take together, lipidomics are developing rapidly, and analysis techniques will also further promote its research and clinical application.关键词：lipids;lipidomics;respiratory system diseases
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发布时间：2024-01-04 - 摘要：Puerarin, a kind of flavonoid compound, was extracted from Puerariae Radix. It showed neuroprotective effects on a variety of brain damage. Studies have found that puerarin had many activities such as reducing the toxic of excitatory amino acids, inhibiting oxidative stress, antagonising Ca2+ overload, reducing inflammation, inhibiting apoptosis, relieving cerebral edema and so on. Multiple studies have demonstrated that puerarin has significant efficacy in the treatment of Parkinson, cerebral infarction, cerebral ischemia syndrome and other diseases of the nervous system. In recent years, the researches relevant to puerarin continue to emerge. This paper provides a summarization of the nearest decade research progress of the puerarin neuroprotective mechanisms, in order to provide some references for studying the neuroprotective effect of puerarin. Through consulting a number of literature materials, the authors found that the study on neural protection mechanism of puerarin in some parts is still not deep enough, especially the genetic level. Neuroprotective mechanisms of puerarin are very complex, and therefore need to strengthen multidisciplinary cooperation to study of its mechanisms in-depth, so as to find more ways of puerarin neuroprotective mechanisms. With the development of modern research methods and techniques of molecular biology, exploring puerarin neuroprotective mechanisms at gene level will be a new orientation. That will bring a new breakthrough for the clinical application of puerarin and explore a new way for prevention and treatment of neurological diseases.关键词：puerarin;neuroprotective;mechanism
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发布时间：2024-01-04 - 摘要：As an early isolated organ perfusion method, the isolated perfused kidney (IPK) has many advantages, such as the removal of nerves and humoral factors, convenience to exert interference factors and real-time monitoring the diversity of renal function. The IPK has great application value in studying drug metabolism, excretion and revelant mechanisms in kidney. This article aims to expand the domestic perspective of using IPK. This paper discussed the applications of IPK in the study of changes in kidney molecules, pathogenesis and interaction of various adjustment factors in nephropathy development process from many aspects, such as regulatory mechanisms of glomerular filtration, reabsorption and secretion of tubule and collecting duct, and renal ischemia injury model. By analyzing a large number of documents, we found that the using of IPK in foreign is relatively mature, while the domestic is more limited, mainly applied in metabolic excretion mechanism of drugs, mechanisms of drug-induced renal injury or donor kidney transplants, etc. This article described the use of IPK technology in the research of the molecular level changes in the kidney and renal mechanisms of pathogenesis. It could provide a reference for the domestic study of changes in renal function and mechanism stimulated by medication and risk factors. It also noted that IPK technology would be very important in morphological and functional changes of podocytes and its molecular mechanisms in the perspective of the whole kidney.关键词：isolated perfused kidney;kidney;renal function
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