最新刊期
卷 21 ， 期 6 ， 2015
- 摘要：Objective: To establish a measuring method for glass transition temperature of traditional Chinese medicine(TCM) extract powder by differential scanning calorimetry(DSC) and provide a reference for application of glass transition theory in solid preparation process of TCM. Method: DSC was developed to determine glass transition temperature of TCM extract powder in order to confirm existence of glass transition phenomenon in TCM extract powder.Single factor tests were adopted to investigate effects of thermal history, flow rate of carrier gas, heating rate, sample volume and other parameters on glass transition temperature, precision of this determination was investigated by Dixion. Result: Nitrogen as a carrier gas, flow rate of carrier gas was 40 mL · min-1, sample amount was 6 mg, heating rate was 10 mg · min-1.Outlier analysis showed under 95% confidence level, determination of glass transition temperature met requirements of test method.Glass transition temperature of Angelicae Sinensis Radix, Astragali Radix, Isatidis Radix, Crataegi Fructus, Lycii Fructus, Chrysanthemi Flos, Rhei Radix et Rhizoma, Shenlingcao were 68.9, 77.0, 75.8, 35.8, 3.4, 56.0, 56.3, 80.0 ℃, respectively. Conclusion: This method is simple with good precision, which can be used to determine glass transition temperature of TCM extract powder.关键词：extract powder of traditional Chinese medicine;glass transition;differential scanning calorimetry;thermal history;Rhei Radix et Rhizoma;Isatidis Radix
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction conditions of volatile oil from Olibanum and Myrrha in Tangshang ointments and analyze its compositions. Method: Taking 1, 1, 1, 2-tetrafluoroethane(R134a) as extraction solvent, with yield of volatile oil as index, based on single factor tests, effects of materials granularity, extration temperature and time on extraction process were investigated by orthogonal test.Composition of volatile oil was analyzed by gas chromatography-mass spectrometry(GC-MS). Result: Optimum conditions was as following:materials granularity of 80 mash, subcirtical extraction temperature of 50 ℃, extraction once for 30 min.Under these conditions, yield of volatile oil from Olibanum and Myrrha was 5.19%.Thirty-nine components were identified by GC-MS, terpenes, aromatic hydrocarbons(hydrocarbons, alcohols, esters) were the major components among them, contents of 1-phenoxy-2-propanone and [1R-(1α, 7β, 8a. α)]-1, 2, 3, 5, 6, 7, 8, 8a-octahydro-1, 8a-dimethyl-7-(1-methylethenyl)-naphthalene were 37.04% and 8.16%, respectively. Conclusion: This extracting techniques is feasible and stable, this study can provide a scientific basis for quality control and further research of Tangshang ointments.关键词：subcritical extraction technology;Olibanum;Myrrha;Tangshang ointments;volatile oil;1,1,1,2-tetrafluoroethane
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发布时间：2024-01-04 - 摘要：Objective: To prepare chitosan-Kudingcha nanoparticles and investigate its physicochemical properties and in vitro release characteristics. Method: Total flavonoids in Ilex kudingcha were extracted by gradient ethanol and its content was determined by UV-spectrophotometer, detection wavelength was 510 nm.Chitosan-Kudingcha nanoparticles were prepared by ionic crosslinking method, morphology, particle distribution, drug-loading, encapsulation efficiency and in vitro release rateof this preparation were examined. Result: The content of total flavonoids in I. kudingcha was 31.84%.These nanoparticles were spherical in shape and the size range was between 200-400 nm, the polydispersity coefficient was 0.137.Optimal prescription of chitosan-Kudingcha nanoparticles was as following:concentrations of chitosan, sodium tripolyphosphate and total flavonoids of 1 g · L-1.In vitro release profile showed that these nanoparticles had sudden release effect in the first two hours with cumulative release amount of 45.6% to 48.9%.Total release amount was 91.9% in 24 hours. Conclusion: Chitosan nanoparticles is an excellent carrier for I. kudingcha.This study can develop a new idea for development of lipid-lowering medicinal preparation.关键词：chitosan;Ilex kudingcha;sodium tripolyphosphate;nanoparticles;total flavonoids
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different permeation enhancer on in vitro transdermal permeation of glabridin through rat's skin and provide a reference for development of transdermal drug delivery system of this compound. Method: HPLC was adopted to determine the content of glabridin with mobile phase of acetonitrile-water-glacial acetic acid(55:44:1) and wavelength at 282 nm.The back skin of rats was used, Franz vertical diffusion cell was adopted to investigate transdermal performance of glabridin, effects of azone, borneol and propylene glycol on transdermal permeation of glabridin were observed. Result: Glabridin was difficult to penetrate the skin by itself, but it could penetrate the skin effectively by adding permeation enhancer.Effect of enhancers on glabridin was ranked as follows:5% azone > 5% propylene glycol > 5% borneol > monomer, permeation rates of steady state were 4.938, 3.041, 2.950 and 2.583 μg · cm-2 · h-1, the lag time were 0.056, 0.180, 0.059 and 0.167 h, respectively. Conclusion: Each penetration enhancers can promote transdermal effect of glabridin, and azone is the best one.关键词：glabridin;Glycyrrhiza glabra;penetration enhancers;transdermal absorption test;permeation rate of steady state;lag time
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发布时间：2024-01-04 - 摘要：Objective: To optimize formula of Yuhong nanoemulsion and provide a reference for improving of quality stability of this preparation. Method: When composition of nanoemulsion changed, conductivity change was observed on the basis of magnetic stirring at a constant temperature, quadratic regression model was adopted to determine critical points of phase transition, then formula of Yuhong nanoemulsion was optimized by making classic ternary phase diagram. Result: Optimum formula was as following:mixed emulsifier with hydrophilic-lipophilic balance value of 11.26, ratio of emulsifier and co-emulsifier 2.603:1, with mixture of glycerol-ethanol(3:1) as a co-emulsifier, refined sesame oil as oil phase, Wahaha purified water as water phase, oil phase-water phase-mixed emulsifier(28.99:63.74:7.27). Conclusion: This optimized formula is stable and feasible with higher drug-loading, it is suitable for improving stability of Yuhong ointments.关键词：Yuhong ointments;ternary phase diagram;nanoemulsion;conductivity
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of starch sodium octenyl succinate(SSOS) inclusion compound of volatile oil from Foeniculi Fructus and investigate its stability. Method: Ultrasonic cell disruption was adopted to prepare SSOS inclusion compound of volatile oil from Foeniculi Fructus, taking comprehensive score of yield of inclusion compound and inclusion rate of volatile oil as index, orthogonal test was employed to optimize inclusion process with working-interval time, total inclusion time and ratio of volatile oil from Foeniculi Fructus to SSOS as factors.Differential scanning calorimetry(DSC), thin layer chromatography(TLC) and infrared spectroscopy(IR) were used for structure characterization of inclusion compound.Stability of inclusion compound was examined under strong light, high temperature and high humidity. Result: Optimum inclusion conditions were as follows:working time of 15 s, interval time of 20 s, total inclusion time of 12 min, ratio of volatile oil from Foeniculi Fructus to SSOS of 1:6.SSOS inclusion compound of volatile oil from Foeniculi Fructus formed successfully.Under affecting factors, the content of volatile oil in inclusion compound decreased 14.5%, 19.5%-38.6% and 15.5%-30.0%, respectively. Conclusion: This optimized inclusion process was stable and feasible with high inclusion rate, it provides a reference for application of SSOS in inclusion process of volatile oil.关键词：starch sodium octenyl succinate;Foeniculi Fructus;volatile oil;differential scanning calorimetry;stability test
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发布时间：2024-01-04 - 摘要：Objective: To optimize formula of Kangfuxin gastric floating tablets and provide a reference for development and utilization of Kangfuxin. Method: Kangfuxin gastric floating tablets were prepared by direct powder compression method.With in vitro floating properties and release as indexes, based on single factor tests, mixture design were adopted to optimize formula by taking dosages of hydroxypropyl methyl cellulose(HPMC), crosslinking povidone(PVPP) and NaHCO3 as factors.HPLC was used to determine the content of amino acids with [0.1 mol · L-1 sodium acetate buffer solution(pH 6.5)-acetonitrile(93:7)]-[acetonitrile-water(80:20)]as mobile phase for gradient elution, detection wavelength of 254 nm and column temperature at 40 ℃. Result: Optimum formulation included 25% freeze-dried powder of Kangfuxin, 28.38% HPMC K4M, 13.38% NaHCO3, 18.24% PVPP, 14% microcrystalline cellulose and 1% magnesium stearate.Kangfuxin gastric floating tablets could float within (2±0.2) min and maintain for over 12 h, in vitro release of this tablets fitted zero-order kinetics model. Conclusion: These prepared gastric floating tablets show a good sustained-release and continual floating property, residence time of Kangfuxin in the stomach increases, it has great significance for treatment of peptic ulcer.关键词：Kangfuxin;gastric floating tablets;mixture design;amino acids;Periplaneta americana;expansive agent;hydrogel matrix
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发布时间：2024-01-04 - 摘要：Objective: The extraction and purification methods for phenolics in Morus alba leaves were studied and the constituents of phenolic were identified, provided suitable experimental drug specimen for exploring the hypoglycemic effect of M. alba leaves. Method: The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index. The factors affecting separation, such as resin dosage, pH and concentration of eluting agent were considerate. The chemical components of phenolic extracted from M. alba were analyzed and identified by UPLC-MS. Result: The optimized extracting conditions were as follows:extracted two times with 70% alcohol, each 1.5 h. The ratio of solvent to material 10:1 and 8:1, respectively;and the purification process with macroporous resin HPD-826 was using 1%Na2CO3, 60% ethanol to wash out phenolics;eight phenolics compounds were identified by UPLC-MS. Conclusion: This method is simple, short cycle, low cost, high content and suitable for industrialized production. This purification technology could be used to extract phenolics of M. alba leaves for studies on its hypoglycemic effect.关键词：Morus alba leaves total phenolic;extraction;purification;qualitative analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish a sensitive and specific HPLC-fingerprint method for the quality control of Trionycis Carapax from different areas. Method: The samples from different areas were determined with the method. Mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the mean chromatogram was also calculated using the 'Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine' (Version 2004A). Samples were clustered using Hierarchical cluster analysis (HCA) and principal component analysis (PCA) based on the ratio of characteristic peaks and standard peak. Result: Seven common peaks in the HPLC-fingerprint of Trionycis Carapax were obtained, and one peak was identified. In addition, all the similarities were over 0.9.12 batches of Trionycis Carapax from different areas were classified into two groups based on hierarchical cluster, which showed the quality of Trionycis Carapax from different areas was in good consistency. The classification result according to PCA was consistent with the HCA. The PCA showed that seven common peaks were integrated into three principal components (PC) and their accumulation contributing rate amounted to 83.21%. Conclusion: SPSS 19.0 software can be used to cluster samples. Its operation is simple and convenient. The result of statistics is reliable and able to provide a reference for the chemometrics classification and quality evaluation of Trionycis Carapax.关键词：Trionycis Carapax;HPLC;chromatographic fingerprint;clustering analysis;principal component analysis (PCA)
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发布时间：2024-01-04 - 摘要：Objective: To establish the fingerprint of the Keteling capsules and content determination of vitexin and isovitexin by UPLC. Method: The Acquity UPLC HSS C18(2.1 mm×100 mm, 1.7 μm)column was used, the column temperature was 25 ℃, the mobile phase consisted of methanol-0.05% formic acid with gradient elution. The flow rate was 0.2 mL · min-1, and the detection wavelength was 325 nm. Fingerprint Similarity Evaluation Software(edition 2012.130723) was used to calculate the similarity and Acquity Waters system was used for the assay. Result: The fingerprint of the Keteling capsules was established, 13 characteristic peaks were identified and the content of vitexin and isovitexin were determined. Conclusion: Established the UPLC fingerprint and content determination methods and reproducibility with good precision and stability, which could manifest the overall quality characteristics of the Keteling capsules. It could provide a scientific basis for the production and quality control of the Keteling capsules.关键词：Keteling capsules;UPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To build up a headspacemethod for detecting organic residues of D-101 macroreticular resin in liquiritin. Method: Using HP-INNOWAX capillary column (0.32 mm×30 m, 0.25 μm).The purpose was to detect hexane, acetone, benzene, toluene, xylene, styrene and other residues, using N, N-dimethyl formamide as solvent. Result: Analytes can be well separated, the RSD were less than 3.0%, peak area and concentration had a good linear relationship, the recoverywas 93.2%-100.7%. Conclusion: This method is stable, accurate and suitable for use D-101 macroporous resin after the detection of residues of organic solvents.关键词：Macroreticular resin;organic residues;gas chromatography;liquiritin
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发布时间：2024-01-04 - 摘要：Objective: To find the steps where problems may occur easily by the method and point out the notes when determining sulfur dioxide in traditional Chinese medicine, thus the operations can be improved and the results can be more accurate. Understanding SO2 pollution of medicinal Codonopsis Radix and Codonopsis Radix samples. Method: The methods for determining sulfur dioxide according to Chinese Pharmacopoeia and European Pharmacopoeia were used to determine sulfur dioxide in Chrysanthemi Flos and Codonopsis Radix. The results and operations were compared, and then the causes of error were analysed. Result: According to the results of Chinese Pharmacopoeia method, 6 of 20 samples of Codonopsis Radix over 400 mg · kg-1, 10 Chrysanthemi Flos samples were under 30 mg · kg-1, it is up to the grade of 150 mg · kg-1. Conclusion: The experimental results show that the starting point of titration, sulfur dioxide absorption process, the titration end point judgment will affect the results, should be strictly controlled. Chrysanthemi Flos of sulfur dioxide pollution less, Codonopsis Radix pollution is relatively serious, we should strengthen supervision and management the market to provide qualified drugs to ensure drug safety.关键词：sulfur dioxide;Chinese pharmacopoeia;European Pharmacopoeia;Codonopsis Radix;Chrysanthemi Flos;contaminant
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of catechin, chlorogenic acid, crocin-Ⅰ, and crocin-Ⅱ in Yinchenhao decoction by HPLC-DAD. Method: The Symmetry C18 column (4.6 mm×250 mm, 5 μm) was used, the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL · min-1. The column temperature was kept at 30 ℃ and the wavelength was set at 202 nm for catechin, 330 nm for chlorogenic acid, and 440 nm for crocin-Ⅰand crocin-Ⅱ, respectively. Result: The linear range of catechin, chlorogenic acid, crocin-Ⅰ, and crocin-Ⅱ were 0.18-0.90 (r=0.999 8), 0.020 8-0.374 4 (r=0.999 9), 0.147-2.646 (r=0.999 9) and 0.007 84-0.141 12 (r=0.999 9) μg, respectively. The mean recovery of these 4 components were 98.56% (RSD 1.8%), 99.07% (RSD 1.8%), 97.41% (RSD 1.9%), and 98.46% (RSD 1.9%), respectively. Conclusion: This method is simple, accurate and specific, and can be used for the determination of 4 constituents in Yinchenhao decoction.关键词：Yinchenhao decoction;catechin;chlorogenic acid;crocin-Ⅰ;crocin-Ⅱ;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC fingerprint of different size of yacon leaves, and determine the content of chlorogenic acid and enhydrin. Method: The fingerprints were obtained on a Kromasil C18 (4.6 mm×250 mm, 5 μm) with the gradient elution solvent system composed of acetonitrile and 0.4% phosphoric acid. The flow rate was 1.0 mL · min-1. The column temperature was maintained at 30 ℃, and the detection wavelength was 0-18 min, 327 nm, 18.1-60 min, 210 nm. Result: The fingerprint of yacon leaves was established. Larger leaves samples with high content of chlorogenic acid(1.588 2 mg · g-1), and the small yacon leaves with high content of enhydrin(5.637 4 mg · g-1). Conclusion: The chlorogenic acid and enhydrin content in yacon leaves was correlated with the size. This method is easy to operate with the high exactitude and the excellent repetition for the quantitative analysis of yacon leaves.关键词：yacon;HPLC fingerprint;chorogenic acid;enhydrin
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the quality of medicinal material of Zanthoxyli Radix in Chinese herbal medicine market. Method: The 18 batches of commercial medicinal material of Zanthoxyli Radix were identified and analysed by macroscopical identification, TLC, HPLC according to the Chinese Pharmacopoeia published in 2010.HPLC fingerprint was applied to evaluate the quality of Zanthoxyli Radix. Result: There were adulterants detected in 9 batches by the macroscopical identification, TLC and HPLC. The total ashes were between 2.4% and 6.4%, the moisture ranged from 7.0% to 12.1%, and the alcohol-soluble extract was in the range of 2.2% to 8.6%. Nitidine chloride was not be detected in 2 batches by HPLC. Compared to the HPLC fingerprint common mode of Zanthoxyli Radix, the similarity of 11 batches were less than 0.8. The 18 batches of samples were not complied with Chinese Pharmacopoeia in 2010. Conclusion: Currently, the quality of medicinal material of Zanthoxyli Radix in Chinese herbal medicine market varies greatly, and the phenomenon of adulterants doping is serious.关键词：Zanthoxyli Radix;Chinese medicinal material;quality evaluation;TLC;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for simultaneous determination of tanshinone ⅡA, tanshinone I, cryptotanshinone and dihydrotanshinone I in Salviae Albae Radix by HPLC. Method: Each of ten batches of Salviae Albae Radix were collected from the same place-Shandong province. A simple and effective high-performance liquid chromatographic (HPLC) method has been developed for simultaneous quantification of four main diterpenes (tanshinone IIA, tanshinone I, cryptotanshinone, dihydrotanshinone I) in Salviae Albae Radix. Chromatography was performed on a (4.6 mm×150 mm, 5 μm) particle size, extend-C18 column. The mobile phase was a linear gradient prepared from 0.2% aqueous formic acid and acetonitrile (43:57) at a flow-rate of 1.0 mL · min-1. Detection was accomplished at 270 nm and column temperature was set at 30 ℃. Result: The contents of tanshinone IIA, tanshinone I, cryptotanshinone, dihydrotanshinone I in Salviae Albae Radix were between 2.677-7.150, 0.320-1.747, 0.916-5.935, 0.132-2.601 mg · g-1, repectively. The average recovery was 100.06%, 99.60%, 99.91%, 100.06%, repectively. Conclusion: The developed method is simple, accurate and good separation, which is suitable for the quality control of tanshinone ⅡA, tanshinone I, cryptotanshinone and dihydrotanshinone I in Salviae Albae Radix.
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发布时间：2024-01-04 -
Determination of Bilobalide, Ginkgolide A, Ginkgolicle B and Ginkgolicle C in Maiping Tablet by HPLC
摘要：Objective: To establish an method for determination bilobalide, ginkgolide A, ginkgolicle B and ginkgolicle C in Maiping tablet. Method: The bilobalide, ginkgolide A, ginkgolicle B and ginkgolicle C in Maiping tablet was determined by HPLC. CAPCELL PAK C18(4.6 mm×250 mm 5 μm)column was used. The modbile phase was tetrahydrofuran- acetonitrile-water(12:10:78)with the flow rate of 1.0 mL ·min-1.The column temperature was 30 ℃ and evaporative light-scatting mass detector. Result: The ginkgolicle C, bilobalide, ginkgolide A and ginkgolicle B were linear at the range of 1.11-6.66, 2.232-13.392, 1.912-11.472, 1.502-9.004 μg, respectively. The average recovery was 96.36%, 97.18%, 97.71%, 96.57%and RSD was 1.0%, 1.4%, 2.1%, 1.4%(n=6). Conclusion: The established method is accurate, reliable and specific. The results are stable with good reproducibility. The quality of Maiping tablet can be controlled by the method.关键词：bilobalide;ginkgolide A;ginkgolicle B;ginkgolicle C;HPLC;Maiping tablet- 3
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发布时间：2024-01-04 - 摘要：Objective: To study the contents and distribution of inorganic elements in Lonicera japonica from different habitats, and analyze the correlations between planting soil. Method: The content of inorganic elements in different parts of L. japonica was determined by atomic absorption spectroscopy and inductively coupled plasma mass spectrometry. The principal component analysis and cluster analysis were conducted by SPSS 19.0. Result: Zn, Fe, K, Cu, Cr and Cd were the characteristic elements of L. japonica. Conclusion: This paper is providing useful information for the quality control of L. japonica.关键词：Lonicera japonica;inorganic elements;principal component analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish optimal inter-simple sequence repeat-polymerase chain reaction(ISSR-PCR) reaction system for Gentiana straminea and provide a reference for medicinal germplasm identification and genetic diversity research. Method: Influencing factors of ISSR-RCR reaction system(template DNA, Mg2+, dNTPs, Taq DNA polymerase, primer and annealing temperature) were selected and optimized by single factor tests and orthogonal test. Result: Optimum reaction system(20 μL) contained template DNA of 1 μL(36 mg · L-1), Mg2+ (10×PCR buffer) of 1.75 μL(20 mmol · L-1), dNTPs of 0.8 μL(10 mmol · L-1), Taq DNA polymerase of 0.1 μL(0.5%) and primers of 0.6 μL(10 mmol · L-1).The suitable annealing temperature of 12 primers was determined, which had rich polymorphism and stable amplification.The best annealing temperature of UBC-809 primer was 55.5 ℃, there were 6 polymorphism bands in amplification of 7 bands, the percentage of polymorphism loci reached 85.71%. Conclusion: This established ISSR reaction system is stable and credible.The polymorphic bands, which is located at 500 bp of strip, can be used as the species characteristic band.关键词：Gentiana straminea;inter-simple sequence repeat reaction system;annealing temperature;UBC-809 primer
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发布时间：2024-01-04 - 摘要：Objective: To determine 22 inorganic elements in the aerial and underground parts of Nardostachys chinensis from different growth stages and investigate the content of heavy metal in N. chinensis. Method: Samples were dissolved by microwave digestion using nitric acid.Contents of Pb and Cd were determined by atomic absorption spectrophotometry(AAS), contents of Hg and As were determined by atomic fluorescence spectrometry(AFS).Contents of Cu, P, S and other elements were determined by inductively coupled plasma atomic emission spectrometry(ICP-OES), plasma radiofrequency transmission power was 1.20 kW, plasma gas flow was 15.0 L · min-1, atomizer gas flow was 0.75 L · min-1, auxiliary gas flow was 1.50 L · min-1, observation height was 10 mm, one reading time was 3.0 s, stability time was 15 s. Result: Contents of Pb, Cd, Hg, As and Cu in different parts of N. chinensis were in line with the 2010 edition of Chinese Pharmacopoeia requirements.Contents of K, Mg, P, Ca, S and Ba were rich, contents of Al, Fe and Na were higher, contents of trace elements of Li, Mn, Sr, Ti, V and Zn were low.Mo was not detected in all samples. Conclusion: Contents of 22 inorganic elements in different parts of N. chinensis are closely related to their growth stages and growing environment, this study can provide a scientific basis for standardized cultivation and comprehensive utilization of N. chinensis.关键词：Nardostachys chinensis;growth stages;medicinal parts;inorganic elements;inductively coupled plasma atomic emission spectrometry
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of artificial vine cutting method on output, quality and seed yeild of Baitiao Codonopsis Radix. Method: By taking seed yield, output and quality as indexes, top of climbing vine of Baitiao Codonopsis Radix was cut when stems and leaves flourished.At the first time the top 30 cm was removed on the middle of July while the second time the top 20 cm was removed on foundation of growth after the first cutting on the beginning of August.Seeds and plants from the middle of October to the beginnning of November were collected, seed yields and outputs per unit and qualities of Baitiao Codonopsis Radix were determined by comparing with the natural growth plants. Result: Seed yields of the plants in the first period were 21.4% over those of the normal plants with natural growth, seed yields of the plants in the second period were 16.5% below those of the normal plants with natural growth.Increase of output per unit in the first period was 25%-35% and artificial vine cutting had little influence on quality of Baitiao Codonopsis Radix. Conclusion: Cutting vine can control output and seed yields per unit area of Codonopsis Radix in Gansu effectively, which can meet rules of quality standards in the 2010 edition of Chinese Pharmacopoeia, this study provides a reference for good agricultural practice(GAP) planting of Baitiao Codonopsis Radix.关键词：regulation technology;Baitiao Codonopsis Radix;seed yield;total ash;extract;lobetyolin
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发布时间：2024-01-04 - 摘要：Objective: To analyze genetic diversities of Mucuna pruriens from different habitats according to their DNA molecule and investigate genetic relationships among them. Method: Inter-simple sequence repeat(ISSR) molecular marker technique was adopted to analyze genetic diversity of M. pruriens from different habitats, ISSR-polymerase chain reaction(PCR) amplification procedure was as following:preliminary denaturation at 94 ℃ for 4 min;40 cycles each involved denaturation at 94 ℃ for 45 s, annealed at 48-54 ℃ for 1 min and extended 90 s at 72 ℃;extended at 72 ℃ for 7 min and kept at 10 ℃.Based on single factor tests, effects of five main reaction elements(Mg2+, Taq DNA polymerase, dNTPs, template DNA and primers) on ISSR-PCR were optimized by orthogonal test.Eleven samples was analyzed by Ntsys-pc and Popgene software. Result: Optimum reaction system was as following:template DNA of 20 ng, ISSR primers of 0.3 μmol · L-1, dNTPs of 0.15 mmol · L-1, MgCl2 of 2.4 mmol · L-1, Taq DNA polymerase of 1 U with 5 U · μL-1, PCR buffer of 2 μL, adding sterilized water to 20 μL.Ten ISSR primers with good repeatability and clear loci were selected from 40 primers and used for ISSR amplication, 97 loci were amplicated, in which 25 were polymorphic loci, accounting for 25.8%.Genetic similarity coefficient of 11 samples was 0.866 0-0.969 1 and samples could be classified into three groups. Conclusion: Genetic variation of M. pruriens has a close relationship with geographical distribution, but genetic variation is small with strong genetic stability.关键词：Mucuna pruriens;inter-simple sequence repeat molecular markers;genetic relationships;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of shikonin on proliferation, invasion and migration of human cervical cancer Hela cells and to explore the possible mechanism. Method: The human cervical cancer Hela cells were cultivated and divided into control group and shikonin group (the concentration was respectively 4, 8, 16, 32, 64 μmol · L-1). The effect of shikonin on cell viability, apoptosis, invasion and migration of cells cultivated after 24 h were studied by MTT assay, flow cytometric and Transwell assay, respectively. When cells were cultivated after 24 h, the expression of focal adhesion kinase(FAK) protein, the level of phosphorylated FAK, and mRNA were assessed by Western blot and quantitative real-time PCR analysis. Result: Shikonin inhibited proliferation and induced apoptosis of Hela cells in a dose-dependent manner. When treated with low-toxic doses of shikonin (4, 8, 16 μmol · L-1), the cell invasion and migration were markedly suppressed. Furthermore, shikonin significantly down-regulated the expression of FAK and the level of phosphorylated FAK. In addition, shikonin could increase the expression of FAK mRNA. Conclusion: Shikonin inhibits proliferation and invasion of cervical cancer Hela cells by inhibiting phosphorylation of FAK.关键词：shikonin;proliferative;invasion;migration;focal adhesion kinase
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Tongxinluo capsule on adhesion molecule in diabetic peripheral neuropathy KK/Upj-Ay mice and its mechanisms. Method: KK/Upj-Ay mice were randomly divided into model, Tongxinluo low-, middle- and high group(4, 2, 1 g · kg-1). C57BL/6 mice were selected as control group. Mice were given intragastrically for 12 weeks. Motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were detected. The content of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) in blood were detected by flow cytometer(FCM). The expression of ICAM-1, VCAM-1 and nuclear factor kappa B (NF-κB) in sciatic nerve were examined by real time PCR and Western blot. Result: Compared with control group, MNCV and SNCV were slower, ICAM-1 and VCAM-1 contents in blood were increased and ICAM-1, VCAM-1 and NF-κB expression in sciatic nerve increased in model group(P<0.01). Compared with model group, MNCV and SNCV were faster and ICAM-1 and VCAM-1 contents were decreased in Tongxinluo middle, high group (P<0.05, P<0.01).The expression of ICAM-1, VCAM-1 and NF-κB mRNA and protein in sciatic nerve of Tongxinluo group significantly decreased (P<0.05, P<0.01). Conclusion: Tongxinluo capsule ameliorates diabetic peripheral neuropathy via inhibiting adhesion molecule expression in the diabetic peripheral neuropathy mice, and this is due to down-regulating NF-κB.关键词：Tongxinluo capsule;diabetic peripheral neuropathy;adhesion molecule;nuclear factor-κB pathway;KK/Upj-Ay mice
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Wenyang Jieyu(WYJY) decoction on behavior and serum cortisol(CORT) in the rat models with chronic stress depression. Method: One hundred and twenty male Wistar rats were randomly divided into six groups:the normal control group, the model control group, high, medium and low dose of WYJY group(8, 6, 4 g · kg-1);and the fluoxetine control group(3.33 mg · kg-1). All rats were fed via intragastric administration at doses of 0.02 mL · g-1. Depression model groups were treated by chronic unpredictable mild stress(CUMS) and single house for 21 d. All rats' behavior changes were observed through open field test(OFT), forced swimming test(FST) and tail suspension test(TST) at 21 d. The serum CORT and adrenocorticotropic hormone(ACTH) level was also detected by radio-immunoassay at 21 d. Result: Compared with the normal control group, the model control group rats' behavior and hormone level in serum were changed significantly(P < 0.05), and WYJY could improve these changes. In FST and TST, the immobility time of the rats in high and medium dose(8, 6 g · kg-1) of WYJY groups were decreased significantly(P < 0.05). In OFT the cross number of the rats in high dose(8 g · kg-1) of WYJY groups were increased significantly(P < 0.05). The serum CORT and ACTH level of rats in high dose(8 g · kg-1) of WYJY groups were decreased significantly(P < 0.05). Conclusion: WYJU can improve the depressive behavior of CUMS rats and it also can reduce the serum cortisol and adreno-cortico-tropic-hormone level.关键词：Wenyang Jieyu decoction;depressive disorder;chronic unpredictable mild stress;cortisol;adreno-cortico-tropic-hormone
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of tetramethylpyrazine(TMP) on the proliferation, migration, tube formation and secretion of the stromal cell-derived factor-1(SDF-1) and vascular endothelial cell growth factor(VEGF) from brain microvascular endothelial cells(BMECs). Method: Primary isolated BMECs of passage 3 to 6 were used in this experiment. BMECs that treated with TMP(2, 10, 50 mg · L-1) were set as TMP 2, TMP 10 and TMP 50 groups, and that treated with the same volume of medium was regarded as control. Effects of TMP on the proliferation of BMECs were detected by MTS and BrdU incorporation. Impact of TMP on migration of BMECs was observed by Transwell assay. The influence of TMP on tube formation of BMECs induced by VEGF was observed by tube formation in vitro. The level of SDF-1 and VEGF in BMECs culture supernatant were examined by ELISA. Result: BrdU incorporation and MTS results showed that TMP(2, 10, 50 mg · L-1) can significantly increase the proliferation of BMECs(P<0.05, P<0.01). TMP can dose-dependently promote BMECs migrate to the lower chamber of Transwell and increase the number of branch points in tube formation. In addition, TMP(2, 10, 50 mg · L-1) can significantly increase the level of SDF-1 and VEGF in cell culture supernatant (P<0.05, P<0.01). Conclusion: TMP could effectively promote BMECs proliferation, migration and tube formation. Meanwhile, it could increase the secretion of SDF-1 and VEGF from BMECs, which may be part of the mechanism for promoting angiogenesis.关键词：tetramethylpyrazine;migration;tube formation;stromal-derived factor-1;vascular endothelial growth factor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the migration of SMMC-7721 hepatocarcinoma cells induced by total paeony glucosides(TPG) and its impact on vascular endothelial growth factor (VEGF), cyclooxygenase (COX) -2 expression. Method: SMMC-7721 cells were cultured with culture solution as blank group and TPG in different concentrations (200, 400, 600 mg · L-1) for 24 h in vitro. Inhibition on proliferation of cells was assessed by MTT assay. Cell migration was detected by wound scratch assay. COX-2 mRNA expression was detected by RT-PCR. VEGF protein expression was detected by Western blot. Result: Compared to the blank group, the proliferation of liver cancer SMMC-7721 cells were significantly inhibited by TPG in a concentration-dependent manner (P<0.05). The cell migration ability was inhibited (P<0.05), the expression of COX-2 mRNA and VEGF protein decreased in the TPG groups. Conclusion: TPG could inhibit the growth and migration of liver cancer SMMC-7721 cells. Its mechanism may be realated to downregulating the expressions of COX-2 and VEGF. The fingdings provide theoretical and experimental basis for the development and clinical application of TGF in treating liver cancer.关键词：total paeony glucosides;SMMC-7721 cells;migration;cyclooxygenase;vascular endothelial growth factor
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to establish a rapid and accurate method in order to evaluate the activity of CYP3A4 by the high-performance liquid chromatography (HPLC). The activity of CYP3A4 was evaluated to explore the protective effect and mechanism of Guipi Tang on rats' acute liver injury which is resulted from the extract from Tripterygium wilfordii by Ethanol. Method: Totally 50 rats were randomly divided into control group, model group, Guipi Tang group, P450 induction group, and P450 inhibition group. The control group and the model group was perfused with physiological saline, the Guipi Tang group was perfused with Guipi Tang (9.0 g · kg-1). The P450 induction group and P450 inhibition group have received injection of DXM(100 mg ·kg-1) and KCZ(80 mg ·kg-1) in intraperitoneal respectively. After ig 4 days, and perfused with T.wilfordii (3.25 g · kg-1) for 3 days, the model was established. The activity of CYP3A4 of rat liver microsome was measured by HPLC with midazolam as a probe drug. Result: The retention time of midazolam appeared at 8.960 min under the condition of HPLC, while there were no interference peaks. Its suitable linear range was 0.25～20 μmol · L-1 (r=0.999 9), which met the requirement of the whole examination. Compared with the control group, the activity of CYP3A4 in model group decreased(P<0.05). Compared with the model group, Guipi Tang and P450 induction group increase the CYP3A4 activity(P<0.05). Conclusion: The evaluation method of CYP3A4 activity is accurate. T. wilfordii can inhibit CYP3A4 activity significantly. Guipi Tang could reduce the toxicity of T. wilfordii and protect liver through inducing the CYP3A4 activity.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism and inhibitory effects of salvianolic acid B (Sal B) on the intermittent high glucose (IHG)-induced Schwann cells (SCs) injury in vitro. Method: SCs were primarily cultured and were divided into various groups, such as normal, high glucose(HG), IHG, osmotic controls, with IHG in the presence of 25, 50, 100 μmol · L-1 Sal B for 48 hrs. Apoptosis, intracellular reactive oxygen species(ROS) generation and mitochondrial transmembrane potential were detected by flow cytometry analysis. The concentration of 8-hydroxy-2-deoxy guanosine (8-OHdG) was detected by enzyme-linked immune sorbent assay(ELISA). Quantitative real-time reverse transcriptase PCR was performed to analyze the expression levels of B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated x protein(Bax) .Western blot were performed to analyze the expression levels of Bcl-2, Bax, Caspase-3 and PARP. Result: The relative levels of intracellular ROS and the percentages of depolarized cells in the IHG group was significantly increased than those in the control and HG group(P<0.05, P<0.01). The concentrations of 8-OHdG showed a marked increase in SCs that were exposed to HG group compared with normal glucose exposure and further increased under IHG conditions(P<0.01). Compared to the normal and HG group, treatment with IHG down-regulated the Bcl-2 expression of protein and mRNA(P<0.01), but up-regulated the Bax expression of protein and mRNA(P<0.01). In addition, treatment with IHG increased the activation of Caspase-3 and the cleavage of PARP in SCs(P<0.01). The percentages of apoptotic cells was increased exposed to HG and substantially more in cells exposed to the IHG(P<0.01). Treatment with Sal B inhibited the IHG-induced oxidative stress by reducing ROS production, 8-OHdG levels, mitochondrial depolarization, and apoptosis in SCs(P<0.01). Furthermore, treatment with Sal B mitigated the IHG-mediated up-regulation of Bax expression and down-regulation of Bcl-2 expression in SCs(P<0.05, P<0.01). In addition, treatment with Sal B attenuated the IHG-induced activation of Caspase-3 and minimized the cleavage of PARP (P<0.01). Conclusion: The cytotoxic effect of IHG may be significantly more potent than that of HG and Sal B antagonized the IHG-induced injury of SCs.关键词：intermittent high glucose;Schwann cells;apoptosis;salvianolic acid B
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Buyang Huanwu Tang (BHT)on the expressions of protein kinase B(AKT)and phosphorylated protein kinase B(p-AKT)in acute cerebral ischemia reperfusion rat. Method: The specific pathogen free SD rats were randomly divided into sham operation group, model group, positive control group and BHT(high, low dose) groups, each group contains 20 rats, Clopidogrel dose was 6.8 mg · kg-1, and BHT high low dose was26, 6.5 g · kg-1. The other groups were given distilled water. The acute cerebral ischemia-reperfusion models were established by bilateral carotid artery occlusion, using HE staining to observe Bcerebral pathological histology, using immunohistochemical method to detect brain cortex and hippocampus CA1 area AKT and p-AKT positive expression level. Result: BHT could significantly improve the degree of acute cerebral ischemia-reperfusion injury in rat brain tissue.HE Staining showed that the number of cortex neuron cells of acute cerebral ischemia-reperfusion rats decreased, cell arranged loosely and not regular, cell nucleus become atrophy, cells surrounding space became big obviously. The number of hippocampus CA1 area neurons decreased significantly, disordered arrangement of cells, and each dose group tissue damage degree were eased. Immunohistochemical results showed that each group of cortex and hippocampus CA1 area were seen AKT(Ab473)and p-AKT (ser473) positive expression. Compared with the sham operation group, model group AKT (Ab473) positive expression had no significant difference. Compared with model group, positive control group, BHT high dose group positive expression of AKT was obviously increased (P<0.05). Conclusion: The neuroprotective effect of Buyang Huanwu Tang against acute cerebral ischemia-reperfusion in rat may be associated with upregulation of AKT and p-AKT.关键词：Buyang Huanwu Tang;acute cerebral ischemia-reperfusion;phosphatidylinositol 3 kinase;protein kinase B
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antithrombotic effect and the mechanism of the thrombolysis effect of mongolian medicine Zhachong Shisan Wei pill. Method: The rats were randomly divided into 5 groups:control group, Zhachong Shisan Wei pill in high, middle and low dose (1.728, 0.864, 0.432 g · kg-1)groups, and compound Danshen dripping pills treatment group with 0.12 g · kg-1.The effects of Zhachong Shisan Wei pill on the formation of thrombus in rats were observed through the way of arteriovenous loop. The dissolution percentage of thrombus in vitro was measured. The fibrinogen degradation product (FDP) of rat at different time after administration was studied in vivo. Result: Compared with the model group, Zhachong Shisan Wei pill with different dose could significantly decrease the weight of thrombus (P<0.001), the length of thrombus (P<0.05, P<0.001).Model group increased the content of FDP compared with the control group. Compared with model group, effect of Zhachong Shisan Wei pill on the FDP content of the thrombus degradation products was decreased(P < 0.05). Conclusion: Zhachong Shisan Wei pill have significant antithrombotic and thrombolysis effects in vivo and in vitro. Degradation of fibrin is one of the mechanisms of antithrombotic and thrombolysis.关键词：Zhachong Shisan Wei pill;antithrombotic effects;thrombolysis mechanism
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发布时间：2024-01-04 - 摘要：Objective: This research was aimed to study effects of tomato leaf aqueous extract (TLAE) against oxidative stress induced by acute inflammatory. Method: Fifty SD rats were randomly divided into control group, model group and three TLAE treated groups of different dose (3.19, 1.59, 0.80 g · kg-1 · d-1). The TLAE was orally given once a day within 10 days. Acute inflammation was induced by intraperitoneal injection of lipopolysaccharide (4 mg · kg-1) after the end of gavage administration. The levels of CuZn-superoxide dismutase(CuZn-SOD), glutathione peroxidase 1(GSH-Px-1), interleukin-6(IL-6) and tumor necrosis factor α(TNF-α) in serum were measured by enzyme-linked immunosorbent assay (ELISA).Gene expressions of CuZn-SOD and GSH-Px-1 in peripheral blood leucocyte were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: TLAE at 3.19, 1.59 g · kg-1 · d-1 enhanced either the gene expressions of CuZn-SOD, GSH-Px-1 or the levels of them in serum. Furthermore, the decreased levels of TNF-α and IL-6 in serum were detected. Conclusion: The orally given TLAE can markedly restrain the oxidative stress companied with the LPS-induced acute inflammatory.关键词：tomato leaves aqueous extract;acute inflammation;lipopolysaccharide;oxidative stress;gene expression
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发布时间：2024-01-04 - 摘要：Objective: To Compare metabolism and tissue distribution in osteoporosis model rats treated by single icariin and compound containing icariin. Method: The rat models of osteoporosis was established by ovariectomy in female SPF rats.Tissues were collected after oral administration of 118 mg · kg-1 icariin 40 min, 1.5, 4, 24 h. The concentration of each component was determined by HPLC after sample preparation. The column was Agilent Eclipse XDB-C18 (4.6 mm×250 mm, 5 μm) and column temperature was 35 ℃;Mobile phase was A(methanol)-B(0.2%H3PO4 in water);the velocity of flow was 1.0 mL · min-1;the detection wavelength was 270 nm. Result: Icariinis widely distributed in rats, high concentration distributed in the kidney, liver, spleen, muscle tissue, and its metabolites coexisted in the organization. Comparison of compound Bushen Zhuanggu and icariinis monomer in the same time and the same organization was executed. In muscle:icariin, icariside Ⅱ, icaritin had a high concentration and slow elimination, icaritin at 4, 24 h and icariside Ⅱat 4 h the significant difference was found(P<0.05). In kidney:icariin at 40 min, 4, 24 h and icariside Ⅰ, icaritin at 24 h was detected, and had a significant difference between groups(P<0.05). In liver:Icariin at 4, 24 h, icaritin at 4, 24 h was detected, the significant difference between groups was found(P<0.05). Conclusion: The distribution of icariin in compound prescription had slower absorption, distribution, longer action time, slower elimination than in single, which may be related to interaction between the chemical composition of compounds.关键词：HPLC;icariin;metabolites;compound compatibility;tissue distribution
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发布时间：2024-01-04 - 摘要：Objective: To explore the immuno-neurologic regulation of renal hypertensive and its inherent law, and to investigate the mechanisms of curing and systemic regulating effect of Ziyin Qianyang formula. Method: Renal hypertension rats (RHR) model was established by two kidney one clip (2K1C) and 2K1C-RHR were divided into six groups:the model group, the high-, medium-, low- dose Zinyin Qianyang formula groups(32, 16, 8 g · kg-1), the losartan potassium group (0.005 g · kg-1) and the Tianma Gouteng particles group (1.5 g · kg-1). Drugs were given by intragastric administration for 4 weeks. The blood pressure was measured by a non-invasive blood pressure monitor. The contents of plasma angiotensin Ⅱ (AngⅡ) were detected by radioimmunity method. The protein levels of angiotensin Ⅱ type l receptor (AT1R) and rabbit-anti protein kinase C (p-PKC) were detected by Western blotting. The contents of inositol triphosphate(IP3) and diacylglycerol(DAG) in peripheral blood lymphocytes and the levels of interlenkin-4(IL-4), IL-6 in the blood serum of RHR were detected using ELISA. Result: Compared with the model group, Ziyin Qianyang formula could decrease blood pressure of RHR significantly (P<0.01). The medium-, low-dose Ziyin Qianyang formula could decrease the concentration of plasma AngⅡ (P<0.05). The high-, medium-dose Ziyin Qianyang formula could decrease the concentration of AT1R and p-PKC in peripheral blood lymphocytes of rats (P<0.05, P<0.01). The high-dose Ziyin Qianyang formula could decrease the concentration of IP3 and DAG (P<0.05, P<0.01) in peripheral blood lymphocytes and the levels of IL-4, IL-6 in blood serum of RHR (P<0.05, P<0.01). Conclusion: The renal hypertensive may be related to the excessive expression of AT1R in peripheral blood lymphocytes and the activation of IP3/DAG signaling pathway, where Ziyin Qianyang formula could exert immuno-neurologic regulation action.关键词：Ziyin Qianyang formula;blood pressure;angiotensinⅡ;peripheral blood lymphocytes;angiotensinⅡtype 1 receptors
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of Gegen Qinlian Tang on periodontitis and atherosclerosis rats. Method: The rat model was induced by ligating the second molars of upper left jaw, feeding high fat diet and medications treatment as a complementary method. Thirty successful model rats were divided into the model group, the metronidazole treatment group (0.05 g · kg-1 · d-1), the atorvastatin group (0.08 g · kg-1 · d-1), the metronidazole combined with atorvastatin group, and the Gegen Qinlian Tang group (0.42 g · kg-1 · d-1) of 6 rats in each group. Another 10 rats were assigned to the normal group and feeding with the common forage. After 4-week treatment of the corresponding drug, the changes of periodontal tissue and abdominal aortic pathology were observed under light microscope, HE staining, respectively. The periodontal pocket depth and attachment loss detection, changes of blood lipid total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) were detected. Result: Compared with the normal group, periodontal pocket depth and attachment loss, levels of lipid TC, TG, LDL increased significantly (P<0.05), HDL level decreased significantly (P<0.05) in the model group. Compared with the model group, periodontal pocket depth and attachment loss, and lipid TC, TG, LDL levels decreased significantly (P<0.05), HDL increased significantly (P<0.05) in all treatment groups. No significant difference was found between metronidazole combined with atorvastatin group and Gegen Qinlian Tang group. HE staining showed the destroyed periodontal inflammation, the separation of junctional epithelium and tooth surface, and the proliferation from epithelial to root. The deep tissue showed a large amount of inflammatory cell infiltration and bone trabecular sparse, alveolar bone surface visible bone resorption, alveolar ridge crest height reduced vertical absorption, periodontal ligament fibroblasts dimensional uneven thickness or fractured, arrangement disorder in the model group. These results had good improvement after treatment, and the results were better in the metronidazole combined with atorvastatin group and Gegen Qinlian Tang group. Conclusion: Gegen Qinlian Tang could improve the periodontal inflammation levels and reduce the blood lipid level of model rats. The results suggest that Gegen Qinlian Tang has a good therapeutic effect on periodontitis associated with atherosclerosis disease.关键词：Gegen Qinlian Tang;atorvastatin;metronidazole;periodontitis;atherosclerosis
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发布时间：2024-01-04 - 摘要：Objective: To verify the objective existence and synergistic effect of key herbs by researching the effect of Cimicifugae Rhizoma and Bupleuri Radix combination in Buzhong Yiqi Tang on spleen deficiency mice. Method: Ninety mice were randomly divided into the control group (CG), the model group (MG), the positive control group (mosapride, 1.93×10-3 g · kg-1) (PCG), Buzhong Yiqi Tang group (BZYQTG, 8.58 g · kg-1), BZYQT minus of Danggui Chenpi decoction group (MDCDG, 7.41 g · kg-1), BZYQD minus of Shengma Chaihu decoction group (MSCDG, 7.02 g ·kg-1), Shengma Chaihu decoction group (SCDG, 1.56 g · kg-1), MSCD plus of Qianghuo Duhuo and Fangfeng decoction group (PQDFDG, 10.53 g · kg-1), MSCD plus of Gegen decoction group (PGDG, 8.32 g · kg-1) of 10 rats each. Except the mice in the normal group, the other mice were induced by a compounding method-administration of Rhubarb decoction, exhaustive swimming and starvation to a spleen deficiency model. After intragastic administration for 7 days, the anti-fatigue functions and other effects including spleen index, thymus index and gastrointestinal propulsion of mice were observed. Result: The swimming time, spleen and thymus weight and small intestinal propulsion rate of MG mice were significantly lower than those of the normal group (P<0.01). The swimming time, thymus weight and the rate of intestinal propulsion had no significant difference between SCDG and MG mice, while the swimming time and small intestinal propulsion rate were higher in other groups than those in MG (P<0.01). The swimming time was significantly longer in BZYQTG mice than PCG (P<0.05), while the results of weight of the spleen and thymus gland, and the intestinal propulsion rate had no significant difference between two groups. The swimming time, spleen and thymus weight and the rate of intestinal propulsion in the prescription drug groups were significantly lower than BZYQTG (P<0.01). The swimming time and the rate of intestinal propulsion were significantly lower in MSCDG than MDCDG (P<0.01, P<0.05). Conclusion: The key herbs of Cimicifugae Rhizoma and Bupleuri Radix combination in BZYQT had more contribution on anti-fatigue effect, improving immune function and gastrointestinal propulsive effect than other adjuvants, which proved that the key synergistic effect of the key herbs. The key herbs of Shengma and Chaihu combination in BZYQT cannot be replaced by Notopterygii Rhizoma, Anoelicae Pubescentis Radix, saposhnikoviae Radix and Puerariae Labatae Radix, which proved that the best compatibility of the drug. This study verified that the key herbs in some formulas are objective existence from the point of view of experimental research.关键词：Buzhong Yiqi Tang;key herb;spleen deficiency;combination
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发布时间：2024-01-04 - 摘要：Objective: To set up an acute liver injury mice model by α-amanitin and evulate the hepatoprotective effect of silibinin. Method: Sixty Kunming mice were randomly divided into 6 groups and intraperitoneally injected with α-amanitin at 0.10, 0.20, 0.35, 0.70, 1.32, 2.50 μg · g-1, respectively. The LD50 and LD90 of α-amanitin were calculated by the improved karber method. Another 120 Kunming mice were randomly divided into 0, 12, 24, 36, 48, 60, 72 h groups, the time-activity curve was established using LD50 α-amanitin. The preventive and curative effects of silibinin were investigated from mortality, prevention group using LD90 α-amanitin. The preventive experiment included different dosage of silibinin groups (10, 25, 50, 100 μg · g-1). The curative experiment included different time point groups (10, 20, 40, 60 min after intraperitoneal injection of α-amanitin) at silibinin of 100 μg · g-1. The death situation of mouse was observed for 7 days. Moreover, 36 Kunming mice were randomly divided into the blank group, the model group, the silibininin treatment group (100 μg · g-1). The activities of aspartate aminotransferase (AST), alanine transaminase (ALT), and level of malonaldehyde (MDA) were detected. Pathological changes of hepatic specimens were observed. Result: The LD50 and LD90 of α-amanitin toxic peptides on acute liver injury in mice was 0.36 μg · g-1 and 0.50 μg · g-1, respectively. Preventive treament with silibinin raised the survival rate of mice. Silibin of 10 μg · g-1 could protect 70%, 25 μg · g-1 could protect all animals against the action of α-amanitin 60 min before administration of LD90 α-amanitin. Curative treatment with silibinin was very effective when administered 10 min after administration of LD90 α-amanitin. The anti-α-amanitin effect diminished when silibinin was 40 min after administration of LD90 α-amanitin. Compared with the blank group, levels of AST, ALT, MDA increased, SOD activity decreased significantly in the model group (P<0.01). Compared with the model group, silibinin could significantly decrease serum ALT and AST levels, increase SOD after administration of LD50 α-amanitin. From the observation of pathological picture, it showed the structure of hepatic lobule was unclear, hepatic cell cords disordered, liver cell edema, part of vacuolar changed, spotty necrosis of liver tissue in the model group. Silibinin could improve the above pathological changes significantly. Conclusion: Silibinin has a good effect in treating the α-amanitin-induced acute liver injury.关键词：α-amanitin;silibinin;acute liver injury;hepatoprotective effect;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: To screen the anti-inflammatory fractions of Gentiana rigescens, and to explore its mechanism. Method: The ear edema model induced by xylene and inflammatory exudates model in the abdominal cavity induced by acetic acid in mice, foot swelling and chest infection model caused by carrageenan in rats were applied to screen the anti-inflammatory effect. The ear edema, inflammatory exudates and foot swelling experiments included the normal group, the model group, the dexamethasone acetate group, the different Fractions of G. rigescens groups. The chest infection experiment included the normal group, the model group, the dexamethasone acetate group, the water and ethanol extract of G. rigescens groups. The anti-inflammatory mechanisms were investigated by detecting the contents of malondialdehyde (MDA), prostaglandins (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β) in exudates, and the contents of nitric oxide (NO), PGE2, TNF-α, IL-1β, MDA in lung tissue. Result: Compared with the normal group, the contents of NO, PGE2, TNF-α, IL-1β, MDA increased in the ear edema, inflammatory exudates, foot swelling and chest infection groups (P<0.01). Compared to the model group, the high-and middle-dose water extracts (6, 3 g · kg-1) had a significant inhibition on all kinds of animal models (P<0.05, P<0.01), and the other extraction had a litter or no activity. The water extraction of Gentiana rigescens of all dose groups reduced the levels of MDA, PGE2, TNF-α, IL-1β in exudates(P<0.01), and reduced the contents of NO, MDA, PGE2, TNF-α, IL-1β in lung tissue significantly (P<0.01). Conclusion: Water fraction may be the anti-inflammatory active site of Gentiana rigescens. The effect may be related to the inhibition of the generation of mediators of inflammation and its antioxidation.关键词：Gentiana rigescens;anti-inflammatory;active fraction;screen;anti-inflammatory mechanism
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发布时间：2024-01-04 - 摘要：Objective: To study the serum containing drug of different processed products of Qing'e Pill on osteoblast proliferation, differentiation and mineralization. This study was designed to evaluate the promoting effect of serum containing drug of different processed products of Qing'e Pill on human osteoblast and investigate the possible mechanism in the treatment of osteoporosis. Method: Osteogenic cells were obtained from the femur in postmenopausal women. Different processed products of Qing'e Pill were performed by using crude and salt-roast Eucommia ulmoides and Psoralea corylifolia, respectively. Female Sprague Dawley rats were orally administrated with water extract of Qing'e Pill for 7 days. At the end of the administration, serum containing drug was collected. The experiment was divided into the blank group, the blank serum group, the different processed products of Qing'e Pill groups. Cell proliferation was determined by MTT assay. Cell (activity of alkaline phosphatase) ALP activity and mineralized nodule were tested using ELISA. Result: Compared to the blank serum group, the different processed products of Qing'e pill could significantly promote huaman osteoblast proliferation and ALP activity (P<0.05). Moreover, the formation of bone nodules increased in the osteoblasts after 14-day treatment in different processed products of Qing'e pill groups (P<0.05). Meanwhile, the ALP (P<0.01) and mineralization activity results of the salt-roast were better than the crude. Conclusion: Qing'e pill could increase proliferation, differentiation and mineralization activities in human osteoblast. Its therapeutic effect of osteoporosis might be involved in increasing the osteogenic function. Comparing to the crude drug, the salt-roast has a better effect in treating osteoporosis.关键词：Qing'e pill;serum containing drug;human osteoblast;osteoporosis
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发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to observe the effect of Yiqi Huoxue decoction on myocardial energy metabolism of rats with congestive heart failure. Method: Eighty rats were subject to exhaustive swimming and hunger to cause heart failure model with coronary artery ligation.Two weeks after operation, the survivals were divided into four groups:model group, Chinese medicine group, western medicine group, and normal group 4 group, treated by gavage or with distilled water for 28 days. We detected the changes of the expression of proteins and genes of peroxisome proliferator activation receptor gamma coactivator 1 (PPAR γ-coactivator-1, PGC-1) by using Western blot and RT-PCR method.The concentration of high-energy phosphate (adenosine triphosphate, ATP) was determined by using colorimetric method, and the contents of serum N terminal pro brain natriuretic peptide (NT proBNP) and cardiac troponin I (cTNI) concentration were determined via ELISA. Result: Compared with normal group, the ATP and PGC-1 α levels of rats in model group and other groups were significantly lower (P<0.01), while the contents of NT-proBNP and cTnI were significantly increased (P<0.01);compared with the model group, the ATP and PGC-1 α levels of rats in Chinese medicine group and western medicine group, increased significantly (P<0.01), the contents of NT-proBNP and cTnI decreased (P<0.01). In western medicine group and Chinese medicine group, there was no significant difference inthe levels of ATP, PGC-1 α, NT-proBNP and cTnI in the serum. Conclusion: Yiqi Huoxue decoction can significantly reduce NT-proBNP and cTnI content in rats with chronic heart failure, improve the energy metabolism, promote the production of PGC-1 alpha, and delay the development of chronic heart failure.关键词：Yiqi Huoxue decoction;chronic heart failure;PPAR γ-coactivator-1α;adenosine triphosphate;N-terminal pro-brain natriuretic peptide;cardiac troponin I
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发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to observe the acute, genetic and long-term toxicity of the Korean Red Ginseng to evaluate its safety. Method: Test of the maximum tolerance dose(MTD) was used to detect the acute toxicity, and the genetic toxicity test was evaluated by Ames test, micronucleus test of born marrow in mice and sperm shape abnormality test in mice). The rats were given Korean Red Ginseng in high, medium and low(6.0, 3.0, 1.5 g · kg-1) dose continuously for 30 days, to detect the long-term toxicity. Then we conducted the effects on blood cytology indexes, blood biochemical indexes, weight coefficient of organs, and the histopathology examination, routine examinations and reversibility study. Result: There was no obvious toxic symptom in the acute toxicity trial (in the rats in different sexual, the MTD were both higher than 12.0 g · kg-1 treated with Korean Red Ginseng). The results of genetic toxicity test were all negative. The results of long-term toxicity trial indicated that there were no significant differences in appearance, behavior, body weight, blood cytology indexes, blood biochemical indexes, organ/body ratio, and pathematology examination in the rats in all the three dosage groups (6.0, 3.0, 1.5 g · kg-1) as compared to the control group. The reversibility study demonstrated that there was no delayed toxicity in the rats in all the three dosage groups as compared to the control group. Conclusion: Korean Red Ginseng is highly safe with neither acute toxicity nor genetic toxicity.关键词：Korean Red Ginseng;acute toxicity test;genetic toxicity test;thirty-day feeding test
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发布时间：2024-01-04 - 摘要：Objective: To discuss the revival effect and the mechanism of Angong Niuhuang pills in treating coma caused by acute cerebral infarction. Method: Eighty patients with coma caused by acute cerebral infarction were randomly divided into the Western medicine group (40 cases) and the experimental group (40 cases) by a random number table. Patients in the Western medicine group received routine Western medical therapy. Based on the treatment of Western medicine group, patients in the experimental group added 3 g Angong Niuhuang pills once daily. All patients in both two groups received 7 days of treatment. Before and 3, 7 days after treatment, scores of Glasgow coma scale (GCS) and the national institutes of health stroke scale (NIHSS) were graded. Brain oedema was evaluated. Levels of high sensitivity C-reactive protein (hs-CRP), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) were detected. Result: The curative efficacy of NIHSS in the experimental group was superior to that in the Western medicine group (P<0.05). At the third and seventh day after treatment, the total scores of eye opening response, verbal response, movement of limbs and GCS in the experimental group were lower (P<0.05), score of NIHSS was lower than those in the Western medicine group (P<0.01). After treatment, levels of hs-CRP and TNF-α were lower (P<0.01), and the level of IL-10 was higher than those in the Western medicine group (P<0.01). Volume of edema decreased more obviously and the amelioration of midline shift was better in the experimental group than that in the Western medicine group (P<0.01). Conclusion: Based on the treatment of routine Western medical therapy, Angong Niuhuang pills could make altered consciousness and ameliorate neurological function of patients. The effect may be related to relieving inflammatory response and edema, and promoting the recovery of neurological function.关键词：Angong Niuhuang pills;acute cerebral infarction;Glasgow coma scale;edema
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical effects of Xuanbi Xiaotong decoction combined with methotrexate tablets in treating rheumatoid arthritis (RA) with damp heat stagnation syndrome, and to observe its influence on anti-cyclic citrullinated peptide antibody (anti-CPP), antifilaggrin antibody (AFA) and tumor necrosis factor-α (TNF-α). Method: Ninety-five patients with RA disease were randomly divided into the control group and the observation group by a digital method. Patients in both groups took 7.5-20 mg methotrexate tablets once weekly, 7.5-15 mg meloxicam once daily. Patients in the control group added 2 pieces of total glucosides of white paeony capsules orally thrice daily. Patients in the observation group added Xuanbi Xiaotong decoction. All patients received 4 months of treatment. The joint time of morning stiffness, rest pain (visual analogue scales), joint tenderness number, swelling index (including 28 joints), swollen joint index, hands grip and healthy activity of life quality (HAQ) changes were recorded. Levels of erythrocyte sedimentation rate (ESR), C reactive protein (CRP), rheumatoid factor (RF), anti-CPP, AFA, TNF-α were detected before and after therapy. Result: The curative effect of the observation group was superior to that of the control group (P<0.05). After treatment, improvements including joint time of morning stiffness, rest pain, joint tenderness number, swelling index, swollen joint index, HAQ and hands grip in the observation group were better than those in the control group (P<0.01). Levels of ESR, CPR and RF in the observation group were lower than those in the control group (P<0.01). Levels of anti-CCP, AFA and TNF-α in the observation group were inferior to those in the control group (P<0.01). Conclusion: On the basis of methotrexate tablets therapy, adding Xuanbi Xiaotong decoction could reduce clinical symptoms and signs, control disease activity, lighten RA bone and cartilage damages. Its clinical effect is superior to using paeony capsules alone.关键词：rheumatoid arthritis;damp heat stagnation syndrome;Xuanbi Xiaotong decoction;anti cyclic citrullinated peptide antibody;antifilaggrin antibody;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To discuss the promoting effect on lower limb motor function in patients with stroke through Yiqi Tongnao decoction, and to discuss its influence on homocysteine (HCY), allergic C-reactive protein (hs-CRP) and D-dimer (D-D). Method: One hundred and six stroke patients during the recovery period were randomly divided into the control group (53 cases) and the observation group (53 cases) by a random number table. Patients in both groups received conventional medical treatment and modern comprehensive rehabilitation training therapy. Patients in the control group added Xingnao Kaiqiao acupuncture therapy. Based on the treatment of the control group, patients in the observation group added Bupi Tongnao decoction. All patients in both groups received 8 weeks of treatment. Lower limb muscle strength, lower extremity motor function Fugl-Meyer (leg), walking ability, walking ability (BBS scale), daily life activity (BI index) and convulsion degree (Ashworth method) were evaluated. Serum HCY, hs-CRP, D-D were detected. Result: Scores of lower limb muscle strength and walking ability in the observation group were lower (P<0.01), scores of BI index and Fugl-Meyer (leg) were higher in the observation group than those in the control group at the fourth and eighth week after treatment (P<0.01). Besides, Ashworth score in the observation group was superior to that in the control group (P<0.01), while muscle strength grading improvement of Ashworth was better than that in the control group (P<0.05). After therapy, BBS score of the observation group was higher than that of the control group (P<0.01). Meanwhile, BBS classification improvement was superior to that in the control group (P<0.05). Levels of HCY, hs-CRP and D-D in the observation group were lower than those in the control group (P<0.01). Conclusion: On the basis of conventional medical treatment, adding Yiqi Tongnao decoction could enhance lower limb muscle strength, reduce the convulsion degree, improve ability of balance, and raise lower extremity motor function and ability of life in stroke patients.关键词：stroke disease;hemiplegia;lower extremity motor function;Yiqi Tongnao decoction;recovery therapy
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发布时间：2024-01-04 - 摘要：Objective: To discuss the curative efficacy of Bushen Tiaochong decoction in treating postmenopausal osteoporosis (PMOP) and to investigate its influence on interleukin-1 (IL-1) and interleukin-6 (IL-6). Method: One hundred and twenty-four patients with PMOP were randomly divided into the control group (62 cases) and the observation group (62 cases) by a random number table. Patients in the control group took 10 mg alendronate sodium tablets and 0.6 g calcium carbonate D3 chewable tablets orally. Based on the treatment of the control group, patients in the observation group added 1 dose Bushen Tiaochong decoction in twice daily. All patients in both two groups received 6 months of treatment. Before and after treatment, score of pain of lower back was graded by visual analogue scales (VAS), bone mineral density of lumbar L2-4 and levels of serous calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), bone gla protein (BGP), estrogen2(E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), interleukin-1 (IL-1) and interleukin-6 (IL-6) were detected. Result: The total effective rate of osteoporosis in the observation group was 91.9%, which is superior to 77.4% in the control group (P<0.05). VAS of pain of lower back in the observation group was lower than that in the control group (P<0.01). After treatment, bone mineral density of lumbar L2-4 was higher (P<0.01), levels of BGP and ALP were lower, and level of Ca was higher than those in the control group (P<0.01). E2 level in both groups increased obviously and the result in the observation group was better than that in the control group (P<0.01). Levels of FSH decreased in both groups (P<0.01). Levels of IL-1 and IL-6 in the observation group were lower than those in the control group (P<0.01). Conclusion: Bushen Tiaochong decoction could improve bone mineral density and relieve pain symptoms of patients with PMOP. The comprehensive curative effect was superior to Western medicine treatment alone, and its mechanism of action may be related to improving the level of estrogenic hormone, inhibiting osteoclast-activating factor, promoting osteogenesis and inhibiting bone resorption.关键词：postmenopause osteoporosis;Bushen Tiaochong decoction;sex hormones;interleukin 1;interleukin 6
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发布时间：2024-01-04 - 摘要：Objective: The aim of this paper was to observe the effectiveness of Bushen Yiqi Huoxue Fang in combination with massage on prophylaxis of deep venous thrombosis(DVT) following postoperative period of aged hip arthroplasty. Method: Ninety patients receiving surgery by the same group of doctors were randomly divided into traditional Chinese medicine (TCM) group, low-molecular-weight heparin(LMWH)group and control group, postoperative patients in TCM group were treated with Bushen Yiqi Huoxue Fang in combination with massage. In the LMWH group, 30 patients were given LMWH after surgery;patiens in the control group took aspirin enteric-coated tablets. The limb circumference, routine blood test, blood coagulation function changes, hemodynamics of vessels by vascular ultrasound and the thrombosis were examined before surgery and one day, 10 days after surgery, respectively. Result: In limb circumference variation, there was significant difference the three groups (P < 0.01). There was no significant difference of platelets, D-dimer (D-D), prothrombin time(PT), activeated partial thromboplastin time(aPTT) in all the three groups in the assay of one day after surgery, while significant difference (P < 0.01) appeared between the TCM group and the control group in the assay of 10 days after surgery. There was significant difference of the occurrence of DVT after surgery between the TCM group and the control group (P < 0.01).In TCM group, one DVT case (3.33%) was discovered. In LMWH group, one DVT case (3.33%) was discovered as well. While in the control group, 6 DVT cases (20.0%) were found. Conclusion: On the basis of this study, it is more effective and safer to use Bushen Yiqi Huoxue Fang in combination with massage on prophylaxis of DVT following postoperative period of aged hip arthroplasty.关键词：deep venous thrombosis;Bushen Yiqi Huoxue Fang;musculus gastrocnemius;quadriceps femoris muscle;aged hip arthroplasty
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this paper was to investigate the clinical efficacy of treatment on the Qiyinliangxu-type lung cancer by chemotherapy with or without Bufei Jiedu soup. Method: Seventy cases of Qiyinliangxu-type lung cancer in middle and late period were randomly grouped into chemotherapy group and Bufei Jiedu soup combined with chemotherapy group, with 35 cases in each group.The chemotherapy groups were only treated by western medicine chemotherapy scheme, while the Bufei Jiedu soup combined with chemotherapy groups were cured with Bufei Jiedu soup based on the treatment of control group.After 4 continuous treatment periods, the short-term curative effect, improvement of life quality, improvement of peripheral blood and immune function, and the occurrence of adverse reaction were examined. Result: The total effective rates in chemotherapy group and Bufei Jiedu soup combined with chemotherapy group were 85.72%and 62.86%, respectively.The short-term curative effect in Bufei Jiedu soup combined with chemotherapy group was significantly better than the chemotherapy group(P<0.05);as to the improvement of life quality, the Bufei Jiedu soup combined with chemotherapy group was significantly better than the chemotherapy group (P<0.05) with the improvement rate of life quality of 62.00% and 33.33%, respectively. The peripheral blood abnormality rates in Bufei Jiedu soup combined with chemotherapy group were significantly lower than the chemotherapy group (P<0.05);the immune indexes (CD4+, CD8+, CD4+／CD8+and the activity of NK cell) were compared before and after treatment in both Bufei Jiedu soup combined with chemotherapy group and chemotherapy group, and all showed significant difference in different degree(P<0.05), so the immunity of Bufei Jiedu soup combined with chemotherapy group was obviously improved. Compared with chemotherapy group, the occurrence rate of adverse reaction in Bufei Jiedu soup combined with chemotherapy group was significantly lower than chemotherapy group (P<0.05). Conclusion: In the case of Qiyinliangxu-type lung cancer, the treatment of chemotherapy with or without Bufei Jiedu soup could effectively relieve the clinical symptoms, improve the immunity, reduce the occurrence of adverse reaction, and improve the life quality, and the TCM treatment combined with chemotherapy had the efficacy improved and toxicity lowered, and it was worthy of promotion and application in clinic。关键词：Bufei Jiedu soup;chemotherapy;Qiyinliangxu type;non small cell lung cancer;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: The aim of this article was to study the efficacy and safety of Aidi injection in combination with radiotherapy in treating non-small cell lung cancer (NSCLC). Method: Cochrane Library, PubMed, CBM, CNKI, VIP and WANFANG DATA were screened. The retrieval time was from the establishment time of each database to May 2014.Randomized controlled trials (RCTs) of Aidi injection in combination with radiotherapy in any language were included. Two researchers extracted data and assessed the literature quality separatedly, and made a Meta-analysis by RevMan 5.2 software. Result: 10 RCTs involving 678 patients entered the inclusion criteria. The meta-analysis showed that compared with pure application of radiotherapy, Aidi injection in combination with radiotherapy can enhance the short-term curative effect [OR=4.00, 95%CI(2.86, 5.60), P<0.000 01], and improve the quality of life [OR=4.99, 95%CI(3.02, 8.26), P<0.000 01], decrease the incidence of radiation pneumonitis [RR=0.46, 95%CI(0.34, 0.63), P<0.000 01] radiation esophagitis [RR=0.49, 95%CI(0.37, 0.66), P<0.000 01] and the bone marrow suppression [RR=0.51, 95%CI(0.38, 0.69), P<0.000 01]. Conclusion: Aidi injection in combination with radiotherapy can not only enhance the short-term curative effect, but also improve the quality of life, and decrease the incidence of radiation pneumonitis, radiation esophagitis and bone marrow suppression. However, the quality of RCTs is low, so more clinical trials in high quality were needed to study.关键词：Aidi injection;radiotherapy;non-small cell lung cancer;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to analyze and summarize medication rules for treating allergic rhinitis with Chinese medicine in literatures in CNKI database based on the traditional Chinese medicine (TCM) inheritance platform system. Method: We collected literatures related to Chinese formulas treating allergic rhinitis from CNKI database, and screened the formulas from the literatures to establish a formula database. Then the medication rules were figured out via the TCM inheritance platform system. Result: Totally 182 formulas used for treating allergic rhinitis were obtained, which involves 177 kinds of Chinese medicines. The medicines which are used to invigorate Qi and consolidate the superficial, and to expell wind and open qiao were most frequently used. The most frequently used medicine match were Astragalus Mongholicus and Radix Sileris, which accounted for 63.2% of all formulas. There were 22 key medicine matches and 11 new formulas discovered from this study. Conclusion: Through analyzing literatures related to Chinese formula treating allergic rhinitis from CNKI database via the TCM inheritance platform system, the main prescriptions were obtained, which are mainly used to invigorate Qi and consolidate the superficial, and to expell wind and open qiao. The prescription rules concluded in this study can provide a reference for clinical treatment.关键词：allergic rhinitis;TCM inheritance platform system;composition principles
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to evaluate the curative effect of the therapy of promoting blood circulation to activate collaterals of traditional Chinese medicine on rheumatoid arthritis through mata analysis. Method: All the relevant domestic studies that have been published from 2000 to 2013 were searched. After evaluation, the eligible ones were subject to Jadad score and meta-analysis by software Rev Man 5.2.The publication bias was analyzed by funnel plots. Result: Totally 10 studies were selected and analyzed, with two in high quality while the others were low quality literatures by Jadad score. The comparision between traditional Chinese medicine (including traditional Chinese medicine in combination with western medicine) and western medicine was as following[OR=3.58, 95%CI(2.37, 5.42), Z=6.03, P<0.000 01]; and the comparison between traditional Chinese medicine and western medicine was as following[OR=4.66, 95%CI(2.38, 9.14), Z=4.48, P<0.000 01]. Funnel plots showed the credibility of meta-analysis. Conclusion: The therapy of promoting blood circulation to activate collaterals is useful for improving symptoms and effective to RA treatment.关键词：rheumatoid arthritis;therapy of promoting blood circulation to activate collaterals;Bi syndrome;Meta-analysis
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发布时间：2024-01-04 - 摘要：Alzheimer's disease (AD) is a neurodegenerative disease with characteristics of progressive cognitive impairment, abnormal behavior and personality change. There is no effective treatment method for AD in a global context as AD is a multifactorially complex disease. Intervention to AD is always late. More and more researchers proposed that early intervention and multiple targetsare needed in AD treatment. In the guidance of the principles of nourishing the kidney, strengthening the essence, and prevention before disease onset, we studied the neuroprtective effects of Jiawei Wuzi Yanzong Fang on mild cognitive impairment. Clinic study showed Jiawei Wuzi Yanzong Fang can improve the clinical symtoms of mild cognitive impairment(MCI) patients, delay the development of MCI to AD, the mechanism is related to the inhibition activity of acetylcholine esterase, and reduce the oxidative damage of DNA in mitochondria and the Aβ level in serum. Imageology study also proved that this prescription can prevent and cure the hippocampal volume shrikage as it can reduce the hippocampal index and the temporal angle width. We also used the method of high performance liquid chromatography-photodiode array detector-tandem mass spectrum (HPLC-PDA-MS/MS) to analyze the prescription's compositions. Then we constructed the HPLC Fingerprint of the Jiawei Wuzi Yanzong Fang. Finally, the further experiments in vivo and in vitro confirmed that the Jiawei Wuzi Yanzong Fang and its effective compositions could improve the AD rats models' behavioristics symptomes, regulate the central nervous cholinergic system, inhibit the impairment induced by Aβ, oxidative stress and inflammation. Thus we concluded that the Jiawei Wuzi Yanzong Fang and its effective compositions could exerts neuroprtective effect at multipletargets and in multiplepathways.关键词：Jiawei Wuzi Yanzong Fang;clinical research;medicinal material basic research;pharmacological research
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发布时间：2024-01-04 - 摘要：Objective: To provide references for researchers of Tripterygium by bibliometric analysis in recent years. Method: Based on China HowNet CNKI resource database, taking built-in analysis tools and network analysis tools, bibliometric analysis on Tripterygium research literatures from 1915 to 2013 about thesis annual trend, research institutes, periodical distribution, researchers, research contents and so on. Result: Tripterygium lacked of a comprehensive system of research, its material basis was not clear;its research direction was mainly focused on pharmacology research, followed by traditional Chinese medicine internal medicine and Chinese medicine chemistry.Study on chemical ingredients of Tripterygium regelii was less, preparations and pharmacy research of T. wilfordii was relatively backward, research of T. regeli and T. hypoglaucum resources were still in blank. Conclusion: There is still a great space for development of resources, preparation, material basis and comparison within genus plants of Tripterygium.关键词：Tripterygium;bibliometrics;research area;journals distribution;database
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发布时间：2024-01-04 - 摘要：Cell signal transduction system has become one of the hot research of diabetic nephropathy(DN) in recent years, as the initiating factor induced by high glucose, protein kinase C (PKC)signaling pathway activation and promote the research development of DN occurred, people pay more attention to the. Many studies show that the abnormal activation of PKC signaling pathway is involved in the occurrence and development of DN, and its important role has gradually established: activation of PKC signal pathway can significantly affect the hemodynamics, increased vascular permeability and extracellular matrix deposition, inhibiting the activity of Na+-K+-ATP and involved in cell proliferation. Opened up a new perspective and approach and the use of PKC inhibitors for the prevention and control of DN. The study confirmed that, Chinese herbs and their extracts, and some traditional Chinese medicine compound has protective effect on DM of rat kidney, also have good inhibitory effect on PKC signal pathway. But the existing research evidence that PKC signaling pathway inhibitors are usually nonspecific, and the side effect is big, but the specificity of PKC signaling pathway inhibitor is still in study, and so far no successful application to clinical precedent. Therefore, with the modernization of traditional Chinese medicine to accelerate, in-depth study of traditional Chinese medicine inhibits the activation of the PKC signaling pathway, has the important meaning to the signal path from intervention in the progress of DN. This paper will present the intervention of traditional Chinese medicine progress in PKC signaling pathway and DN research, for the future use of traditional Chinese medicine as PKC inhibitor for the prevention of DN and provide experimental and theoretical basis.关键词：protein kinase C;diabitic nephropathy;signaling pathway
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