最新刊期
卷 20 ， 期 10 ， 2014
- 摘要：Objective: To compare active ingredients contents,HPLC fingerprints and pharmacodynamics activities of different Andrographis Herba extracts. Method: Extracts were prepared by three different extraction technologies of vacuum-assist extraction(VAE),ultrasound-assist extraction(UAE) and heating reflux extraction(HRE),extraction efficiencies and micro-fingerprints analysis of extracts were investigated by HPLC,taking acetonitrile(A)-0.2% formic acid(B) (0-20 min,10%-30%A;20-30 min,30%-35%A;30-40 min,35%-60%A;40-50 min,60%-85%A;50-60 min,85%-100%A) as mobile phase for gradient elution,detection wavelength was set at 254 nm.Antioxidant activities of extracts were investigated by DPPH free radical reaction system. Result: Compared with traditional methods,extraction efficiencies of active ingredients(andrographolide and dehydroandrographolide) were higher by VAE.Analysis of HPLC fingerprint showed 17 main characteristic peaks in three different extracts,similarities of VAE,HRE and UAE were 0.957,0.911 and 0.905,respectively.DPPH free radical scavenging capacity was in the order of VAE>UAE>HRE,when the concentration of active ingredients was 20 g·L-1,DPPH clearance rates were 44.85%,25.29% and 16.58%,respectively. Conclusion: Active ingredients in different extracts of Andrographis Herba had no significant differences.Compared with traditional extracting methods,VAE had advantages,such as short extraction time,high efficiency,no pollution and so on.Scavenging effect of VAE solution on DPPH radical was better than HRE extract and UAE extract.关键词：Andrographis Herba;fingerprint;DPPH;vacuum-assist extraction process;andrographolide;dehydroandrographolide
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发布时间：2024-01-04 - 摘要：Objective: To determine apparent solubility and apparent oil/water partition coefficient of flavonoid components from Epimedii Folium,so as to lay foundation for overall solubility characterization of these components. Method: Taking flavonoid components from Epimedii Folium as model drug,HPLC was adopted to determine equilibrium solubilities and apparent oil/water partition coefficients(Papp) of epimedin A,B,C and icariin in different pH buffers by taking mobile phase of acetonitrile-water(25:75) and detection wavelength at 270 nm. Result: Overall equilibrium solubilities of flavonoid components from Epimedii Folium in different pH buffers were in order of epimedin B>epimedin A>epimedin C>icariin,oil/water partition coefficients were -1.437-3.147,overall trends of Papp were changed from small to large and then to small,to large at last. Conclusion: Water-soluble and fat-soluble of flavonoid components from Epimedii Folium were well with a similar overall trend,epimedin B belonged to slightly soluble,epimedin A,C and icariin belonged to very slightly soluble.关键词：Epimedii Folium;flavonoid components;equilibrium solubility;oil-water partition coefficient;epimedin A,B,C;icariin
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发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasonic extraction technology of rosmarinic acid,salvianolic acid B,cryptotanshinone and tanshinone ⅡA from Salviae Miltiorrhizae Radix et Rhizoma. Method: With extraction amounts of four active components as indexes,uniform design was adopted to optimize extraction technology by taking ethanol concentration,solid-liquid ratio,extracting time as factors.HPLC was employed to determine contents of four active components with mobile phase of methanol-0.01% phosphoric acid solution(0-3 min,30%A;3-5 min,30%-40%A;5-13 min,40%A;13-20 min,40%-58%A;20-22 min,58%-75%A;22-24 min,75%A;24-50 min,75%-85%A) for gradient elution and detection wavelengths at 286 nm(rosmarinic acid and salvianolic acid B) and 268 nm(cryptotanshinone and tanshinone ⅡA). Result: Optimum extraction conditions were as follows:ethanol concentration of 75%,liquid-solid ratio of 200 mL·g-1,extraction time 10 min;Under these conditions,extraction amounts of rosmarinic acid,salvianolic acid B,cryptotanshinone and tanshinone ⅡA were 0.33,11.19,0.63 and 0.59 g·g-1,respectively.Compared with heating reflux extraction in 2010 edition of "Chinese Pharmacopoeia",Contents of four bioactive components had no significant differences. Conclusion: This optimum extraction process had advantages of low mammalian toxicity,solvent saving,fast extracting speed and so on.关键词：Salviae Miltiorrhizae Radix et Rhizoma;uniform design;ultrasonic extraction process;active components;rosmarinic acid;salvianolic acid B;Cryptotanshinone;Tanshinone ⅡA
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发布时间：2024-01-04 - 摘要：Objective: To predict shelf life of Aconiti Radix Cocta and provide basis for assessing its quality. Method: Contents of benzoylaconine,benzoylhypacoitine,benzoylmesaconine,aconitine,hypaconitine and mesaconine were determined by HPLC,with acetonitrile(A)-40 mmol·L-1 ammonium acetate(B) (0-45 min,30%-45%A;45-65 min,45%-50%A;65-75 min,50%-55%A;75-80 min,55%-65%A;80-85 min,65%A) as mobile phase by gradient eluting,detection wavelength was set at 240 nm.Initial average rate test was adopted to predict shelf life,contents change of diester alkaloids and monoester alkaloids in Aconiti Radix Cocta under different temperature were investigated. Result: Shelf life of Aconiti Radix Cocta had been determined as 0.69 years. Conclusion: High temperature was not conducive to stability of Aconiti Radix Cocta,initial average rate test could provide a reference for establishing validity of herbs.关键词：initial average rate test;shelf life;Aconiti Radix Cocta;diester alkaloids;monoester alkaloids
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of compound Smilacis Glabrae Rhizoma granules. Method: With overall desirability(OD) of extraction rate of astilbin and total flavonoids,dry extract yield as dependent variable,Box-Behnken response surface methodology was employed to optimize extraction technology by taking extraction times,extraction time and solid-liquid ratio as independent variables,each level of independent variables was fitted by multiple linear regression and binomial formula fitting.HPLC was adopted to determine the content of astilbin with mobile phase of acetonitrile-0.1% phosphoric acid(20:80) and detection wavelength at 290 nm. Result: Optimum extraction technology was as following:extracted 3 times with 9.8-fold the amount of water for 1.4 h each time;under these conditions,extraction rates of astilbin and total flavonoids were 1.991 and 54.436 mg·g-1,dry extract yield was 8.558%,relative deviation between the observed and predicted values was 1.54%. Conclusion: Box-Behnken response surface methodology was suitable for optimizing extraction technology of compound Smilacis Glabrae Rhizoma granules.关键词：compound Smilacis Glabrae Rhizoma granules;Box-Behnken response surface methodology;astilbin;total flavonoids;dry extract yield;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total polysaccharides from Atractylodis Macrocephalae Rhizoma. Method: With the content of total polysaccharides as index,effects of the amount of water,extraction time and temperature on extraction technology were investigated by uniform design.UV was adopted to determine the content of total polysaccharides by detection wavelength was 491 nm. Result: Optimum extraction technology was as following:extracted 60 min with eight times the amount of water at 90 ℃;The content of total polysaccharides in water extract of Atractylodis Macrocephalae Rhizoma was 6.20%. Conclusion: Optimized extraction process was stable and feasible,this established determination was accurate and reliable.关键词：Atractylodis Macrocephalae Rhizoma;extraction technology;uniform design;Ultraviolet spectrophotometry;methodological study
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Diphylleia sinensis stir-baked with vinegar. Method: Taking the content of podophyllotoxin as index,orthogonal test was adopted to optimize processing technology with four factors including moistening time,the amount of vinegar,frying time and temperature.With mobile phase of methanol-0.1 mol·L-1 potassium dihydrogen phosphate solution(45∶55) and detection wavelength at 290 nm,the content of podophyllotoxin was determined by HPLC. Result: The best processing technology was as following:moistened 2 h with 30% vinegar,flew 4 min at 150 ℃.The amount of vinegar and frying time had significantly effects on processing technology,the mass fraction of podophyllotoxin was up to 5.48%. Conclusion: This optimized processing technology was reasonable and feasible.This result could provide a reference for medication safety of D. sinensis in folk.关键词：stir-baked with vinegar;Diphylleia sinensis;podophyllotoxin;Orthogonal Design;processing technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total flavonoids from poplar buds. Method: With the content of total flavonoids from poplar buds as index,based on single factor tests,orthogonal test was adopted to optimize extraction technology by taking ethanol concentration,extraction time and solid-liquid ratio as factors. Result: Optimum process determined by orthogonal test was as following:extracted tiwce with 13 times the amount of 90% ethanol for 30 min per time;The content of total flavonoids was 28.82%.The best process optimized by central composite design-response surface methodology was as following:extracted twice with 14 times the amount of 80% ethanol for 24 min per time;The content of total flavonoids was 31.14%. Conclusion: Central composite design-response surface methodology was better than orthogonal method in accuracy,combined two methods for optimizing application of extraction process for traditional Chinese medicine had good predictability.关键词：orthogonal design;central composite design-response surface methodology;total flavonoids from poplar buds;extraction technology;UV spectrophotometry
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Jiawei Guizhi Fuling decoction. Method: Orthogonal test was adopted to investigate effects of the amount of water,extraction times and timeon extraction process by taking composite score of extraction amount of caffeic acid,forsythin,forsythoside A and paeoniflorin.HPLC was employed to determine contents of index components with detection wavelength were 323,277,330, 230 nm,respectively. Result: Optimal extraction ntechnology was as following:decocted 3 times with eight times the amount of water for 2.0 h each time;extraction amount of caffeic acid,forsythin,forsythoside A and paeoniflorin were 1.04,7.80,12.37,35.33 mg·g-1,respectively. Conclusion: This optimized extraction technologywas reasonable and scientific by multi-index comprehensive score.关键词：Jiawei Guizhi Fuling decoction;extraction technology;multi-index comprehensive score;caffeic acid;Forsythin;forsythoside A;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To optimize bran-roasted processing technology of Chebulae Fructus. Method: With composite score of appearance and the content of free gallic acid as index,orthogonal design was adopted to optimize processing technology by taking bran amount,processing time and temperature as factors.HPLC was empolyed to determined the content of gallic acid with mobile phase of acetonitrile-0.1% formic acid(3:97) and detection wavelength at 273 nm. Result: Optimal processing technology was as follows:roasted 20 min with 0.4 times the amount of bran at (140±5) ℃;the content of gallic acid was up to 27.62 mg·g-1. Conclusion: Optimized processing technology was stable and feasible,which could provide process parameters for industrial production and quality control of Chebulae Fructus.关键词：Chebulae Fructus;processing technology;orthogonal test;gallic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of total flavonoids from Dracocephalum moldevica-phospholipids complex. Method: Solvent evaporation method was adopted to prepare total flavonoids from D. moldevica-phospholipids complex.Based on single factor tests,with recombination rate as dependent variable,central composite design was adopted to optimize preparation technology by taking reaction time,ratio of total flavonoids to phospholipids and the concentration of total flavonoids as independent variables,fitting of various mathematical equations were performed using a statistical software of Design-Expert 8.0.6.Preparation parameters were optimized through response surface plotted by optimum fitting equations,optimized procedure was validated through experimental preparation of total flavonoids-phospholipids complex. Result: Optimum preparation technology was as following:reaction temperature 40 ℃,with methanol-dioxane(7:3) as a reaction solvent,ratio of phospholipids to total flavonoids of 2:1,total flavonoids concentration of 15 g·L-1 and reaction time 2 h;Under these conditions,recombination rate was up to (96.42±1.58)%,which had deviation of <2.0% between the observed value of recombination rate. Conclusion: Optimized preparation technology was feasible,reliable and suitable for industrial production of total flavonoids from D. moldevica-phospholipids complex.关键词：total flavonoids from Dracocephalum moldevica;phospholipids complex;central composite design;response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To optimize adsorption conditions of geniposide on SP825 macroporous resin and investigate its adsorption equilibrium,thermodynamics and kinetics. Method: Based on single factor tests,response surface methodology was adopted to optimize adsorption conditions of geniposide with adsorption rate as index,isotherm adsorption model,kinetic curve and thermodynamic parameters of geniposide on SP825 resin were measured. Result: Optimum adsorption conditions were as follows:the concentration of sample solution 83 g·L-1,pH of 2.79;The maximum adsorption capacity was 247 mg·g-1.Adsorption equilibrium was well fitted by Freundlich formula,and kinetics process could be formulated by pseudo-second equation. Conclusion: Adsorption of geniposide was an spontaneous exothermic process,which could benefit from low temperature,adsorption rate was mainly controlled by liquid membrane diffusion.关键词：geniposide;Macroporous resin;response surface methodology;adsorption dynamics;adsorption thermodynamic;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To study the variations of different soaking marinated time on the alkaloids in aconite root, and the content of 6 alkaloids in different soaking marinated time samples of aconite root was analyzed by high performance liquid chromatography (HPLC). Method: The content of alkaloids in aconite root was determined by HPLC with Waters Sunfire C18 column (4.6 mm×250 mm, 5 μm). The mobile phase consisted of acetonitrile-tetrahydrofuran (25:15) and 0.1 mol·L-1 ammonium acetate solution (adding glacial acetic acid 0.5 mL into 1 000 mL solution) with the flow rate was 0.8 mL·min-1. Detection wavelength was set at 235 nm. Column temperature was fixed at 30 ℃. Result: The content of mesaconitine, hypaconitine, aconitine and benzoylmesaconine in mud aconite decreased gradually when it was soaking in the Danba solution. After 20 days, compared with it before processing, the content of four compounds in processed aconite fell by 60.39%, 73.84%, 72.57% and 88.46% respectively. Conclusion: It can prevent mud aconite decaying when it was soaking in Danba solution. After 20 days, the quality of processed aconite was stable, and both of the content of diester-type alkaloids and monoester-type alkaloids fell by about 70%. In order to ensure the clinical medication safety and effectively, it is necessary to improve the processing method of mud aconite.
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC-DAD method for qualitative and quantitative analysis of pharmacodynamic components in CO2 supercritical fluid extract of Chuanxiong Rhizoma. Method: HPLC-DAD was employed by Elipse XDB-C18 column(4.6 mm×250 mm,5 μm) with 0.3% acetic acid solution(A)-acetonitrile(B) as mobile phase for gradient elution(0-4 min,40%-41.5%B;4-10 min,41.5%-70%B;10-15 min,70%B),detection wavelengths of ferulic acid,senkyunolide A and ligustilide were set at 325,328,280 nm,respectively. Result: Three compounds from CO2 supercritical fluid extract of Chuanxiong Rhizoma could be separate well within 15 min,linear range of these three ingredients were 0.024-2.4,0.15-15,0.3-30 μg,average recoveries were 95.8%(RSD 0.99%),96.85%(1.04%) and 102.2%(RSD 1.29%),contents of these three compounds in Chuanxiong Rhizoma were 0.35%,3.13% and 7.17%,respectively. Conclusion: This established method was simple,rapid,objective and reliable,which was suitable for overall quality evaluation of CO2 supercritical fluid extract of Chuanxiong Rhizoma.关键词：Chuanxiong Rhizoma;CO2 supercritical fluid extraction;ferulic acid;senkyunolide A;ligustilide
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发布时间：2024-01-04 - 摘要：Objective: To establish an UPLC method for the content determination of aspidin BB in Dryopteris fragrans. Method: Determination was performed on XBridgeTM BEH C18 column (4.6 mm×100 mm,2.5 μm) with mobile phase consisted of acetonitrile-0.1% trifluoroacetic acid with detection wavelength at 290 nm. The flow rate was set to 0.8 mL·min-1,while the column temperature was set at 40 ℃. Result: The linear range of aspidin BB was 0.050 28-0.301 68 μg(r=0.999 8)with an average recovery of 98.87%(RSD 0.44%,n=6). Conclusion: The method is fast,accurate and reproducible, which can be used to control the quality of D. fragrans.关键词：UPLC;Dryopteris fragrans;aspidin BB;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish the methods for extraction,isolation and determ ination of ellagic acid from Turpinia arguta, and to compare its contents from different areas. Method: The ellagic acid reference substance was isolated by solvent extraction, macroporous adsorption resin, reverse phase silica gel column chromatog ramphy and recrystallization techniques. Then determined it by the method of HPLC from T. arguta, the chromatographic column was UltimateXB-C18 column(4.6 mm×250 mm,5 μm),the mobile phase was acetonitrile-0.1% phosphoric acid, the flow rate was at 1.0 mL·min-1, the detection wavelength was 254 nm and the column temperature was 30 ℃. Result: The purity of ellagic acid obtained was higher than 98.5%,and the average recovery was 101.25%.The good linearity was achieved in the range of 0.020 0-0.400 8 μg. Ellagic acid content of T. arguta from 10 different areas was between 0.37% and 1.56%. Conclusion: Ellagic acid obtained from the method was with high purity, and it can be adopted as the reference substance. This method was simple,rapid and accuracy, it can be used to determine the contents of ellagic acid from T. arguta .The contents of ellagic acid were different in T. arguta from different areas. The result provided a reference for development of T. arguta resource.关键词：Turpinia arguta;ellagic acid;extraction and isolation;determination
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发布时间：2024-01-04 - 摘要：Objective: To analyze and compare the chemical components of volatile oil in Cinnamomum camphora chvar borneol leaf from Jiangxi Ji'an,Hunan Xinhuang, Zhejiang Chun'an and Fujian Xiamen. Method: The volatile oil was extracted from C. camphora chvar borneol leaf by steam distillation. The components of volatile oil were separated and analyzed by GC-MS. Result: 44 components in the volatile oil of C.camphora chvar borneolleaf form Jiangxi Ji'an were identified and accounted for 99.16% of the total components which include 53.17% d-borneol. 33 components in the volatile oil of C. camphora chvar borneol leaf from Hunan Xinhuang were identified and accounted for 97.84% of the total components which include 32.71% d-borneol. 34 components in the volatile oil of C. camphora chvar borneol leaf form Zhejiang Chun'an were identified and accounted for 98.1% of the total components which include 41.89% d-borneol. 25 components in the volatile oil of Cinnamoumum camphora chvar borneol leaf form Fujian Xiamen were identified and accounted for 99.58% of the total components which include 60.74% d-borneol. Conclusion: There are some differences in the component and content in volatile oil of C. camphora chvar borneol leaf from different habitats.关键词：Cinnamomum camphora chvar borneol leaf;different habitats;volatile oil;d-borneol;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish an RP-HPLC method for simultaneous determination of contents of the four flavonoids, astilbin, neoisoastilbin, quercetin-3-O-α-L-rhamnoside, engeletin, contained in Smilax china. Method: Hypersil C18 (4.6 mm×250 mm,5 μm) column was used with the mobile phase of acetonitrile-water (0.04% phosphoric acid) at a flow rate of 0.7 mL·min-1 and the detection wavelength of 290 nm. External standard method (astilbin, neoisoastilbin, quercetin-3-O-α-L-rhamnoside, engeletin used as the reference substance) and quantitative analysis on multi-components by single marker method (astilbin used as the internal reference substance) were adopted to determinate the contents of the four flavones in S. china.Result: The four flavonoids showed good linear relationship (r>0.999 9) within the linear range of calibration curve by the external standard method. The average recoveries of astilbin, neoisoastilbin, quercetin-3-O-α-L-rhamnoside, engeletin were 100.8%, 100.3%, 101.4%, 101.4%, respectively;and the RSD of the above four components were 1.8%, 2%, 1.4%, 1.6%, respectively. The relative correlation factors (RCF) of neoisoastilbin, quercetin-3-O-α-L-rhamnoside, engeletin were 0.726, 0.264, 0.801, respectively and the RSD were 0.6%, 0.7%, 1.2%, 1.1%, respectively by the quantitative analysis on multi-components by single marker (QAMS) method. No significant difference between the contents of QAMS method and external standard method was observed. Conclusion: The present research established an HPLC method proved to be easy and accurate to determine the contents of the four flavones in S. china. There is no significant difference between the contents of the four flavones in S. china. in the results of QAMS method and external standard method. The QAMS method could replace the external standard method to determine the contents of the four flavones in S. china and can be used for the quality control of S. china.
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发布时间：2024-01-04 - 摘要：Objective: To determine the five anthraquinones in Rheum officinale and formulation by the method named quantitative analysis of multi-components by single marker(QAMS). Method: A HPLC-DAD method was developed to determine the contents of five anthraquinones, and QAMS methods were established when the five components were used as internal referring substances, respectively. The relative correction factors (RCFs) between the five anthraquinones (aloe-emodin, rhein, emodin, chrysophanol, physcion) were calculated and used to assay the contents of five anthraquinones in different batche R. officinale and formulations, and compared standards' calibration curves (SCCs) method to evaluate the accurary of QAMS method. The ratio of relative retention time was adopted to identify the accurate position of five anthraquinones. Result: When emodin as internal referring substance, the ranges of the relative error between QAMS and SCCs in different batches Rheum officinale and extrations and preparations were -3.91%-4.06%,-3.22%-3.27%,-5.39%-7.00%, respectively. The RSD of the relative retention value were 3.87%-9.92%. Conclusion: A rapid,accuracy, low-cost and convenient QAMS method can be used as a new quality assessment model for quanlity analysis of anthraquinones in Rheum officinale and formulation.
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for determination of the content of genistin,genistein, protocatechuic acid and gallic acid in Mazongshe Yaojiu(MZSYJ). Method: An Hypersil C18 column was used as the chromatographic column, the flow rate was 1.0 mL·min-1. Genistin and genistein:the mobile phase A consisted of acetonitrile-methanol(2:1),the mobile phase B consisted of 0.2% formic acid solution, the UV detection wavelength was at 261 nm. Protocatechuic acid and gallic acid:the mobile phase A consisted of methanol, the mobile phase B consisted of 0.3% phosphoric acid solution. The UV detection wavelength was at 268 nm. Result: There were a good linear relationship between the concentration of genistin, genistein and peak area value when the concentrations of genistin and genistein were within the range of 0.010 4-0.208 0 μg(r=0.999 3), 0.004 8-0.096 0 μg(r=0.999 6). The average recovery were 97.18%(RSD 1.52%) and 96.94%(RSD 1.40%). There were a good linear relationship between the concentration of protocatechuic acid, gallic acid and peak area value when the concentrations of protocatechuic acid and gallic acid were within the range of 0.038 2-0.764 0 μg(r=0.999 1),0.011 6-0.232 0 μg(r=0.999 4).The average recovery were 95.97%(RSD 1.10%) and 98.39%(RSD 1.45%). Conclusion: The method was accurate, sensitive, reproducible and may be used in the of genistin, genistein, protocatechuic acid and gallic acid in MZSYJ.关键词：Mazongshe Yaojiu;genistin;genistein;protocatechuic acid;gallic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for simultaneous determination of 5-HMF, harpagoside, acteoside, angoroside C, cinnamic acid, methylophiopogonanone A and methylophiopogonanone B in Zengye Decoction. Method: A Diamonsil C18 (4.6 mm×250 mm, 5 μm) was used;The mobile phase was acetonitrile-0.02% formic acid in gradient elution at flow rate1.0 mL·min-1 detected at 296 nm;The column temperature was maintained at 30 ℃. Result: The linear range (mg·L) of 5-HMF, acteoside, angoroside C, harpagoside, cinnamic acid, methylophiopogonanone A and methylophiopogonanone B was 4.12-165.00, 3.98-159.20, 6.97-278.80, 7.25-290.00, 4.29-171.60, 0.07-2.80, 0.12-5.00. The average recoveries were 97.42%, 96.97%, 97.58%, 96.94%, 99.16%, 99.46% and 100.33% respectively. Conclusion: The developed method is convenient, reliable and accurate for the quality control of Zengye Decoction.关键词：Zengye Decoction;5-HMF;harpagoside;acteoside;angoroside C;cinnamic acid;methylophiopogonanone A;methylophiopogonanone B
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprint of Danqi Tablets. Method: The separation was performed on Shiseido CAPCELL PARK C18(4.6 mm×250 mm,5μm)column with mobile phase of acetonitrile-0.06% phosphate (gradient elution) at flow rate of 1.0 mL·min-1.Column temperature was 30 ℃ and detection wavelength was set at 210 nm. Fingerprint of 10 batches of Danqi tablet was analysed by 2004A edition of chromatographic fingerprint similarity evaluation system Result: Fifteen common peaks were selected as the fingerprint peaks. from 10 batches of Danqi tablet and seven of these common peaks were identified as Salvianic acid A sodium, protocatechualdehyde, rosmarinic acid, alkannic acid, ginsenoside-Rg1, salvianolic acid B and ginsenoside-Rb1. Conclusion: The method established is simple, selective, accurate and reproducible, which can be used for the quality control of Danqi tablets.关键词：Danqi tablets;fingerprint;salvianic acid A sodium;protocatechualdehyde;rosmarinic acid;alkannic acid;ginsenoside-Rg1;salvianolic acid B;ginsenoside-Rb1
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发布时间：2024-01-04 - 摘要：Objective: To conduct a quantitative analysis on the volatile oil in Alpiniae Oxyphyllae Fructus,lay the foundation for clinical pharmacodynamic study in the future. Method: The volatile oils were extracted by steam distillation, analysyed main chemical composition by GC-MS. Result: 122 chemical composition was isolated from the Alpiniae Oxyphyllae Fructus, 42 among the 122 composition were identified, accounted for 74.35% of the total volatile oil. There are 9 composition content higher than 1%, the highest composition is 1,2,4,5-tetramethylbenzene (42.96%). Conclusion: The major components of Alpiniae Oxyphyllae Fructus are 1,2,4,5-tetramethylbenzene(42.96%), myrtenal(4.66%), linalool(4.34%), (-)-4-terpineol(2.96%), g-terpinene(2.21%), (+)-nootkatone(1.48%), β-pinene(1.32%), (+)-(4R)-limonene(1.25%), (1S)-(+)-3-carene(1.02%). Most of 122 volatile oils are unsaturated olefin, aldehydes, ketones, phenols with less 15 carbon.关键词：Alpiniae Oxyphyllae Fructus;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides crenatoside and acteoside in Orobanche cumana. Method: The analysis was performed on a Agilent ZORBAX SB-C18 column (4.6 mm×250 mm,5 μm) with CH3CN-0.01%HOAc(18:82) as mobile phase at a flow rate of 1.0 mL·min-1and at a column temperature of 30 ℃,330 nm as the detection wavelength,the well separation was achieved in 30 min. Result: Crenatoside showed good relationship at the range of 1.08-16.2 μg(r=0.999 9),and acteoside at the range of 1.00-15.0 μg (r=0.999 8). The average recovery were 97.23% and 96.27%,relative standard derivation (RSD) of 1.21% and 1.66% for crenatoside and acteoside. Conclusion: The proposed method is simple,rapid,accurate and reliable.It can be used for the quality evaluation of O. cumana and its preparation.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the factors that affect the effective content in Diascorea zingiherersis and control its quality of D. zingiberensis. Method: According to the method in the Chinese Pharmacopoeia(edition 2010),the water determination was adopted.The content of diosgenin was determined by HPLC. On the basis,the relation of diosgenin content with some factors were analysied,such as resh samples water content,different growth years,different producing areas and different growth soil. Result: The results showed that diosgenin content of D. zingiberensis planted in the sandy landwas relatively high,and generally,the diosgenin content reached the maximum after growth in 3-5 years,the content of diosgenin showed positive linear correlation with water content,but the content diosgenin of D. zingiberensis in three producing areas had little difference. Conclusion: These data provide the reference for standardized planting of D. zingiberensis.关键词：Dioscorea zingiberensis;diosgenin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To find the optimal heterologous expression conditions and purification system for the new gene CPS4 from Salvia miltiorrhiza. Method: Compare two different prokaryotic expression strains and optimizing the induction conditions including A600 values of Escherichia coli, isopropyl-beta-D-galactose and glycosides(IPTG) concentration and induction time with orthogonal experiment. The recombinant protein was purified by HisTrap HP. Result: E. coli Tuner (DE3) induced more soluble recombinant protein than BL21 (DE3). The optimal expression conditions are A600 is 0.7, IPTG concentration is 0.2 mmol·L-1 and induction time for 10 h. The recombinant protein was purified by gradient elution with HisTrap HP. Conclusion: This optimal system can obtain enough protein for further study and provide a reference for the prokaryotic expression of the other diterpene synthase.关键词：Salvia miltiorrhiza;prokaryotic expression;orthogonal experiment;purifying
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents of volatile oil from Rosa sterilis by GC-MS. Method: The volatile oil was extracted from R. sterilis by supercritical CO2 extraction. Its chemical constituents and relative contents were analyzed and identified by GC-MS. Result: Thirty-three compounds were identified, representing 91.48% of the total composition. The main components of the oil were β-sitosterol (14.49%), hentriacontane (13.82%), octacosane (7.57%), hexanoic acid (6.80%), 11-(pentan-3-yl)henicosane (6.75%), tetratetracontane (6.56%). In addition, squalene (3.19%) and lupeol (1.18%) were also detected. Conclusion: The study provide a scientific basis for the further exploitation and utilization of the fruit.关键词：Rosa sterilis;supercritical carbon dioxide extraction;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish the simultaneous determination of tanshinone ⅡA,cryptotanshinone,salvianolic acid B,rosmarinicacid, harpagoside,notoginsenoside R1,ginsenoside Rg1,Rb1 and Re in Fufang Xueshuantong tablets. Method: HPLC was performed on C18 column(4.6 mm×250 mm,5 μm) by gradient elution with 0.05% phosphoric acid solution as mobile phase B and acetonitrile as mobile phase A.The column temperature was 20 ℃.The flow rate was 1.0 mL·min-1 with the detection wavelength at 203,270 nm. Result: tanshinone ⅡA,cryptotanshinone,salvianolic acid B,rosmarinicacid, harpagoside,notoginsenoside R1,ginsenoside Rg1,Rb1, Re are in the determination of a good linear relationship within the scope. Recovery rate of the determination method corresponded to the experimental standard. Conclusion: The method is simple, sensitive,accurate,separable and reproducible,and can be used to control the quality of Fufang Xueshuantong tablets.关键词：Fufang Xueshuantong tablets;Tanshinone ⅡA;Cryptotanshinone;salvianolic acid B;rosmarinicacid;harpagoside;notoginsenoside R1;Ginsenoside Rg1;ginsenoside Rb1;Ginsenoside Re
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发布时间：2024-01-04 - 摘要：Objective: To estabolish a quantitative analysis method for pharmacokinetics and bioavailability of puerarin self-microemulsions in rats. Method: Puerarin self-microemulsions and suspensions were given by a single dose,HPLC was adopted to determine the concentration of puerarin in plasma with detection wavelength at 250 nm and mobile phase of methanol(A)-0.1% phosphoric acid solution(B) for gradient elution(0-30 min,20%-25%A;30-40 min,25%-40%A;40-50 min,40%A),pharmacokinetics parameters and bioavailability was calculated by DAS 2.1.1 programs. Result: tmax of puerarin self-microemulsions and suspensions were 0.71,1.1 h,Cmax were 2.052,1.120 mg·L-1,AUC0-24 h were 2.901,2.013 mg·h·L-1, respectively.Relative bioavailability of puerarin self-microemulsions to suspensions was 144.11%. Conclusion: Compared with puerarin suspensions,puerarin self-microemulsion could significantly improve bioavailability in rats.关键词：puerarin;self-microemulsion;pharmacokinetics;bioavailability
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发布时间：2024-01-04 - 摘要：Objective: To study the analgesic effects and mechanism of Wutou decoction. Method: Healthy SD male rats were randomly divided into normal group, model group, Wutou decoction high-dose group, Wutou decocotion middle dose group, Wutou decoction low-dose group and ibuprofen group. SD rats were injected with complete Freund's adjuvant (CFA) into the right ankle joint cavity to induce chronic inflammatory pain in joint. Wutou decoction at the doses of 4, 2, 1 g·kg-1, ibuprofen at the doses of 30 mg·kg-1 were daily ig administrated to rats from day 1 after the CFA injection for 6 days. Joint swelling, cold pain threshold, mechanical pain threshold and thermal radiation pain threshold of the diseased ankle joints were measured. The level of prostaglandin E2(PGE2)and 5-hydroxytryptamine (5-HT) in plasma were detected by enzyme-linked immunosorbant assay, and the levels of transient receptor potential vanilloid 1(TRPV1) and transient receptor potential melastatin 8(TRPM8) protein in dorsal root ganglion (DRG) measured by immunohistochemistry. Result: The ankle joints of rats in model group were obviously swollen, and the cold pain threshold, mechanical pain threshold and thermal radiation pain threshold were significantly decreased compared to the rats in normal group. The levels of PGE2 and 5-HT in plasma and the expression level of TRPV1 in DRG were significantly increased too, and production of TRPM8 in DRG is decreased. Compared with the model group, Wutou decoction high-dose group and middle dose group reduced the joint swelling (P<0.05), all dose group significantly increased cold pain threshold (P<0.01 or P<0.05), high-dose group and middle dose group increased mechanical pain threshold (P<0.01,P<0.05), and high-dose group reduced thermal radiation pain threshold (P<0.05). The levels of PGE2, 5-HT and TRPV1 are decreased while the level of TRPM8 is increased after the treatment of Wutou decoction. Conclusion: Wutou decoction has the analgesic effect, and the mechanism may be related to inhibiting the level of PGE2 and 5-HT in plasma and regulating the expression of TRPV1 and TRPM8 in DRG.关键词：Wutou decoction;chronic inflammatory pain;analgesic;transient receptor potentid vanilloid 1
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity of different fractions from Solanum rostratum in vitro. Method: Antioxidant activities of different extraction fractions of S. rostratum, with vitamin C(VC) as positive control, were evaluated by methods of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, radical scavenging and ferric reducing antioxidant power (FRAP). Result: The results of these methods were similar, and different extraction fractions of S. rostratum had highly antioxidant activities. The antioxidant activity of ethyl acetate extraction was the strongest. Its 50% scavenging rates (IC50) on DPPH and ABTS were 38.428,29.175 mg·L-1, respectively, and the ferric reducing antioxidant power value was 3.774 mmol·g-1 FeSO4 equivalent weight, which were weaker than positive control VC (10.551, 8.570 mg·L-1, 22.795 mmol·g-1). Antioxidant activity of n-butanol extraction was secondly strong, and the IC50 values on DPPH and ABTS were 53.142, 57.895 mg·L-1, respectively, and the ferric reducing antioxidant power value was 1.936 mmol·g-1 FeSO4 equivalent weight. The antioxidant activities of different extraction fractions from strong to weak were as follows:VC>ethyl acetate extraction>n-butanol extraction>the total extraction>petroleum ether extraction>water extraction. The antioxidant properties of different fractions were dose-dependent. With the increasing of the concentrations of various fractions, the antioxidant capacities were enhanced. Conclusion: Ethyl acetate extraction, n-butanol extraction and the total extract of S. rostratum had strong antioxidant activity. The results will be benefit for the exploitation and utilization of S. rostratum.关键词：Solanum rostratum;antioxidant activity;DPPH;ABTS;FRAP
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发布时间：2024-01-04 - 摘要：Objective: To investigate anti-inflammatory of Juanbi particle and its relative mechanisms, to clarify the scientific basis of superior efficiency of Chinese medicine in the clinical treatment on Bi Syndrome (wind-cold-dampness Bi) according to Wu School based on strengthening yang theory. Method: The model of carrageenan-induced edema of rat hind and formation of cotton ball granulom in rats were adopted to investigate anti-inflammatory of Juanbi particle, rats were divided into five groups, including the model group,the positive control group(dexamethasone, Dex, 2.5 mg·kg-1), Juanbi particle group(5.85,11.70,23.40 g·kg-1).Type Ⅱ bovine collagen(CⅡ)-induced arthritis (CIA) in DBA/1 mice were established for investigating the antiarthritic potential of Juanbi particle. The histological damage was assessed by histological examination (HE), the inflammatory cytokine production levels of interleukin(IL)-6,IL-17A and tumor necrosis factor-α(TNF-α) in CⅡ-induced splenic lymphocytes was detected by cytometric bead array (CBA). Result: Compared with the model group,high dosage group of Juanbi particle and positive control group were significantly inhibited swelling of paw and the formation of cotton ball granuloma(P<0.05); meanwhile the clinical score and inflammatory cells infiltration in CIA mice were significantly reduced in the Juanbi particle administration group. Compared with the normal group,there are significantly increased the inflammatory cytokine level of IL-17A ,IL-6 and TNF-α induced by CⅡ (P<0.01). Compared with the model group ,the inflammatory cytokine level of IL-17A was reduced by the Juanbi particle (P<0.05), while the IL-6 and TNF-α have no significant changed. Conclusion: Juanbi particle has an anti-inflammatory effect, the mechanism of which may be related to suppress inflammatory cytokine production levels of IL-17A.关键词：Juanbi particle;collagen-induced arthritis;anti-inflammatory;interleukins-17A
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发布时间：2024-01-04 - 摘要：Objective: Mice Type2 allergic contact dermatitis induced by fluoresciniso thiocyate (FITC) was utilized in this study to explore the effect of astragaioside Ⅳ(AS-Ⅳ) on allergic inflammatory. Method: The BALB/c mice were topically sensitized on the shaved abdomens with 1.5% FITC solution at day 1 and day 2 and elicited on the right ear with 0.5% FITC at day 6.Mice in the dexamethasone group were intraperitoneal injection with dexamethasone(0.67 mg·kg-1) and the AS-Ⅳ group were intragastrically administered with astragaioside Ⅳ(6.25,12.5,25,50 mg·kg-1) from day 1 to day 5 (induction phase). Auricle swelling was measured 24 h after the elicitation. Pathohistological examination was carried out by HE staining.Interleukin-4( IL-4),IL-13 and IL-9 levels of ear tissue homogenates were detected by ELISA. Result: Compared with the normal group, ear swelling in model group was stimulated by FITC treatment significantly and ear tissue local edema, angiectasis and lymphocytic infiltration were observed simultaneously. Meanwhile, IL-4,IL-13 and IL-9 levels increased remarkably in FITC treated mice. Wherein, AS-Ⅳ 25 mg·kg-1 and 50 mg·kg-1 evidently inhibited the auricle inflammation of the FITC treated mice. Pathohistological results indicated that AS-Ⅳ alleviated local edema and angiectasis of mice models and reduced lymphocytic infiltration significantly. Furthermore, IL-4,IL-13 and IL-9 levels in ear tissue homogenates were markedly decreased by AS-Ⅳ treatment. Conclusion: Administration with AS-Ⅳ in induction phase could inhibit Th2 cell-mediated allergic contact dermatitis in mice significantly and this effect was related with regulation on type 2 cytokines.关键词：astragaioside Ⅳ;allergic contact dermatitis;interleukin-4;interleukin-13;interleukin-9
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发布时间：2024-01-04 - 摘要：Objective: To study the resistant effects of Penthorum chinese extract on nonalcoholic fatty liver and possible therapeutic mechanism. Method: The experimental rats were divided into 6 groups:the control group,the model group,fenofibrate group(33 mg·kg-1),P. chinense high-dose,middle-dose and low-dose group(16.7,8.4,4.2 g·kg-1).Except the control group,nonalcoholic fatty liver model was induced by fatty emulsion intragastrically for 9 weeks, those groups were given relevant medicine intragastrically.At the end of 9 weeks, alanine aminotransferase (ALT),aspartate aminotransferase(AST), total bilirubin(TBIL),cholesterol(CHO), triacylglycerol(TG),high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C) in blood were examined.Total choesterol(TC),TG,free fatty acid(NEAF), malondialdehyde(MDA),total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)in hepatic tissue were detected,and the hepatic tissue pathological changes were observed. Result: Compared with the control group, all the indexs showed significant difference(P<0.05 or P<0.01),the content of ALT,TBIL,TG,LDL-C in serum and TG,NEAF,MDA in hepatic tissue increased obviously,HDL-C decreased significantly,and the activity of GSH-Px decreased apparently.And pathological examination indicated steatosis appeared.Compared with model group,the content of ALT,TBIL,CHO,TG in the serum decreased in P. chinense groups(16.7,8.4,4.2 g·kg-1),and HDL-C improved a little,TG and NEAF in the hepatic tissue decreased obviously, the activity of GSH-Px improved significantly(P<0.05 or P<0.01).The pathological images of hepatic tissue and classified graded method of steatosis improved. Conclusion: P. chinese can treat nonalcoholic fatty liver.Its therapeutic mechanism is partly related to regulating lipid metabolism and anti-oxidative damage.关键词：nonalcoholic fatty liver;Penthorum chinese;lipid
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of triterpenoid saponins(H1) from Ardisia gigantifolia on the proliferation of different tumor cell lines. We select the A549 lung cancer cell line as target cell and detect the influence of triterpenoid saponins H1 on cell apoptosis and cell cycle. Method: The three different concentration of H1 (1.25, 2.5, 5 μmol·L-1), were applied to target cells for 24, 48, 72 hours respectively. The control group was not treated. The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric method was used to detect the tumor cell line proliferation and select the cell lines sensitive to H1.The fluorescent inverted biological microscope was used to observe cell morphology and cell apoptosis and cell cycle were detected by flow cytometry instrument. Result: After comparing the IC50 of H1 acted on the target cell line(MCF-7, HepG2, HeLa, A549, Bel-7402 and BGC-823) for 24, 48,72 h, the A549 cell line was proved to be the most sensitive one, a certain concentration of H1 intervention on A549 cells can lead to typical morphological changes and early apoptosis. Cell block was occurred in G2 period, which makes S period increased dramatically. The difference between Medium and high dose group and the negative control group differences were statistically significant. Conclusion: The H1 in a certain range of doses can inhibit the proliferation of six different tumor cell lines and select the A549 tumor cell as target on cell apoptosis and cell cycle.The mechanism of apoptosis induced by A549 cell may be related to cell cycle arrest.关键词：Ardisia gigantifolia;triterpenoid saponins;antitumor activity;apoptosis;cell cycle
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发布时间：2024-01-04 - 摘要：Objective: To explore the effects of lycopene on ischemia reperfusion injure in heart of isolated guinea pig. Method: Thirty isolated guinea pig hearts were divided into five groups:control group, model group, lycopene L group(2.5 mg·L-1), lycopene M group(5 mg·L-1), lycopene H group(10 mg·L-1). Heart rate(HR), coronary flow(CF), infarct size, lactate dehydrogenas (LDH) in effluent, malondiadehyde (MDA) and superoxide dismutase (SOD) in heart tissues were measured in different groups. Result: The indexes of each group are changed after reperfusion, when reperfusion 60 min, compared with the control group, model group showed that HR was decresed (125±9),(169±16)stroke per·minute-1, P<0.01,CF decreased(3.48±0.43),(5.53±0.30)mL·min-1, P<0.01; infarct size increased (19.57±2.34)%,(1.57±0.29)%, P<0.01. the level of LDH increased(206.67±11.85),(120.96±13.56)U·L-1,P<0.01, SOD activity decreased(99.14±8.24),(153.22±8.93)U·mg-1,P<0.01; the level of MDA increased(6.17±0.73),(3.80±0.41)μmol·g-1,P<0.01.Compared to model group, Lycopene group reduced infarct size and releases of LDH in effluent and MDA in heart tissues, increased HR, CF and the activity of SOD in heart tissues. Conclusion: The findings indicate that in the isolated guinea pig heart, lycopene can protect myocardium against ischemia/reperfusion injury and the mechanism could be related to lipid peroxidation in heart.关键词：lycopene;ischemia reperfusion;isolated heart;lipid peroxidation
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发布时间：2024-01-04 - 摘要：Objective: To ascertain the main active site of Typhae Pollen in effect of antithrombosis. Method: Screening for effect of platelet aggregation in vitro from the different extracts of Typhae Pollen(petroleum ether,ethyl acetate,butanol,water)was carried out,further study the effects of the active sites of Typhae Pollen on the collagen-adrenaline and carrageenan induced thrombosis of mice. Effect of effective parts of Typhae Pollen for the rats of thrombosis and vascular wall in vivo. Result: The ethyl acetate parts of Typhae Pollen on arachidoric acid(AA),adenosine diphosphate(ADP),collagen,thrombin induced rabbit platelet aggregation inhibition rates were 40.08%,33.09%,37.91%,34.71%,29.29%,compared with the water parts were 28.65%,28.33%,32.29%;while the protection rate of collagen-adrenaline induced death and paralysis in mice by the ethyl acetate parts of Typhae Pollen was 50%,carrageenan-induced mice black tail incidence was 50%;the site of ethyl acetate have significantly inhibitive effect on the thrombosis models in vivo,and significantly protective effect on vascular endothelial. Conclusion: The site of ethyl acetate is the main active site of the Typhae Pollen.关键词：Typhae Pollen;effect of antithrombosis;active site
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发布时间：2024-01-04 - 摘要：Objective: To investigate the bacteriostatic activity of water extracting-alcohol precipitating solution of Schisandrae Chinensis Fructus. Method: Extracting the active compounds by the method of water extracting-alcohol precipitating, with reference culture strains including Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella and wild E.coli as tested bacteria, the antimicrobial activity variation tendency of the extract of Schisandrae Chinensis Fructus and ciprofloxacin at incubation time 16,32,48, 64 h was monitored by the method of cup plate assay method. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by two-fold dilution method. Result: Extract of Schisandrae Chinensis Fructus had a notable inhibiting microorganism activity to all tested bacteria. Extract of Schisandrae Chinensis Fructus exhibit great superiority compared to ciprofloxacin in inhibiting the ciprofloxacin-resistant strains. The antimicrobial activity variation of Schisandrae Chinensis Fructus and ciprofloxacin showed a gradual decline tendency. Conclusion: The study indicates that extract of Schisandrae Chinese Fructus could be a new antibacterial agent due to its broad-spectrum bacteriostatic activity and the inhibiting activity to resistant bacteria.关键词：Schisandrae Chinensis Fructus;water extract-alcohol precipitating;bacteriostatic activity;variation tendency;ciprofloxacin
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of serum containing Huiyao Aikang formula on apoptosis of human lung A549 cells in vitro. Method: SD rats were divided into low, medium, high dose Huiyao Aikang formula groups, cyclophosphamide(CTX) group;low, medium, high dose combined with CTX group, and normal saline group.The serum containing Huiyao Aikang formula was prepared. The cells were divided into treatment and control groups, the treatment groups were treated with 5%, 10%, 20% containing drug serum, control group was given the same concentration of control serum, at 24,48,72 h the proliferation of A549 was measured by MTT method. The apoptosis rate was measured with flow cytometry(FCM). Result: Compared with control group, apoptosis rates in 5%,10%,20% containing drug serum groups were increased'(P<0.05). At 72 h, the apoptosis rate in low, middle, high dose of Huiyao Aikang formula groups was lower than that in CTX groups.(P<0.05); Conclusion: Huiyao Aikang formula can induce apoptosis of human lung adenocarcinoma A549 cells.关键词：Huiyao Aikang formula;medicated serum;A549 cells;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the in vitro effects and interactions of active fractions in Yaotongning capsule (YTNC) by utilizing interleukin-1β (IL-1β) activated chondrocytes. Method: Six samples were prepared by mixing different active fractions including alkaloids, flavonoids, saponins, volatile oil, hydrous extract and polysaccharides according to the formulating principle and the ratio of materials in YTNC. Chondrocytes were obtained from six seven-day rats using collagenase II, before they were divided into three groups:control group (Dulbecco's modification of Eagle's medium Dulbecco (DMEM) containing 5% fetal bovine serum (FBS) and 0.02% Tween-80), model group (DMEM containing 5% FBS, 0.02% Tween-80 and 10 μg·L-1 IL-1β) and sample group (DMEM containing 5% FBS, 0.02% Tween-80, 10 μg·L-1 IL-1β and 9.77-0.08 mg·L-1 samples). After 48 h, the proliferations of chondrocytes were examined through cell counting kit-8 (CCK8) assay, when the intracellular level of glycosaminoglycan (GAG) was detected by 1, 9-dimethylmethylene blue staining using a glycosaminoglycans (GAG) assay kit. A quantitative evaluation method was exerted to investigate the effect of each sample on IL-1β activated chondrocytes. Result: The comparison of EC50 of YTNC sample and its partial prescriptions indicated that YTNC prescription had the most significant therapeutic effect on promoting proliferation and GAG synthesis in chondrocytes. Synergistic, antagonistic and additive action all could be observed followed by adding alkaloids, flavonoids, saponins, volatile oil, hydrous extract and polysaccharides to each partial prescription. Synergistic and additive effects between active fractions in YTNC prescription were observed, respectively. Conclusion: The therapeutic effect on osteoarthritis (OA) of YTNC without Chinese rice wine was obviously improved after the addition of Chinese rice wine, which indicated that the combination of active fractions in YTNC was reasonable.关键词：proliferation;glycosaminoglycans;osteoarthritis;Yaotongning capsule;active fractions;Chinese rice wine
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Keluoxin capsule on hypoxia-induced angiogenesis in retina. Method: In vitro, cultured ARPE-19 was pretread with 5, 10, 20 mg·L-1 Keluoxin for 24 hours, before 150 μmol·L-1 CoCl2 treatment for 24 hours, ELISA and Western blotting were used to detected the level of vascular endothelia growth factor(VEGF) and hypoxia inducible factor 1 alpha(HIF1-α), expressions of VEGF and HIF1-α mRNA were measured by real-time PCR. In vivo, sixty C57BL/6J mice were randomly divided into six groups as follows:control group, retinopathy(OIR) model group, calcium dobesilate group and Keluoxin groups. In control group, mice were raised in regular air environments. The fifty mice were fed under the environment with 75%±2%oxygen for five days and then returned to normal air to establish OIR model. The 0.5 g·kg-1 calcium dobesilate was administered by gavage feeding once daily in calcium dobesilate group, and 0.5, 1.0, 2.0 g·kg-1 Keluoxin were gavaged as low, middle and high dosage Keluoxin groups respectively. Five days later, the mice were sacrificed using excessive anesthesia method and the retinal sections were prepared for the calculation of the number of endothelial cell nuclei broken retinal inner membrane after hemotoxylin and eosin staining. The morphologic changes of retinal vessels were observed after ADPase staining. Result: The VEGF secretion, mRNA expression of VEGF and HIF1-α of CoCl2 group were significantly higher than control group(P<0.001), and those were decreased by 10, 20 mg·L-1 Keluoxin remarkably (P<0.01 and P<0.05). In vivo, there was a obvious reduction in expansion, deformation and A value of vascular in Keluoxin groups compared with OIR model group, and the number of cell nuclei broken the inner limiting membrane had decreased in Keluoxin groups(P<0.01) significantly. Conclusion: Keluoxin capsule can inhibit hypoxia-induced VEGF secretion and neovascularization in retina.关键词：Keluoxin capsule;VEGF;retinal neovascularization;HIF1-α
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发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro radical scavenging activity and in vivo antioxidant activities of ethanol extracts from Chaihu Shugan powder (CSPEE). Method: The antioxidant activities of CSPEE in vitro were measured by the methods of 1,1-diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl(DPPH)free radicals scavenging capacity, hydroxyl radicals scavenging capacity, and superoxide anion radicals scavenging capacity. The antioxidant activities of CSPEE was studied in vivo, which is compared with the aqueous extracts of Chaihu Shugan powder. Kunming mice were used to study the catalase (CAT) and superoxide dismutase(SOD) activities. Result: The antioxidant activities in vitro showed that free radical scavenging activity of CSPEE was higher. In the results of the animal experiments, the CAT and SOD activities in liver tissue were increased by CSPEE. It was suggested that the antioxidant ability of CSPEE was better than the aqueous extracts at the same drug concentration. Conclusion: The CSPEE owns good antioxidant activities.关键词：Chaihu Shugan powder;antioxidation activities;free radicals
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发布时间：2024-01-04 - 摘要：Objective: To observe the apoptosis inducing effect and inhibitory effect of pachouli alcohol on the proliferation of human androgen independent prostate cancer cell line DU145, and its mechanisms. Method: After prostate cancer DU145 cells were treated with pachouli alcohol for 24, 48, 72 h, the apoptosis morphology was observed by transmission electron microscope;cell viability was estimated using MTT assay;cell apoptosis rate was measured by flow cytometry. Western blot was used to test Caspase-3, Bcl-2, Bax and Livin protein expression. Result: Apoptosis of DU145 cells was detected by transmission electron microscopy after using pachouli alcohol. In vitro, pachouli alcohol significantly inhibited the proliferation of DU145 cells in dose and time-dependent manners which was detected by MTT method. The apoptosis rate was increased significantly which compare with the control group. Pachouli alcohol could enhance Caspase-3, Bax protein expression, and reduce Livin, Bcl-2 protein expression in DU145 cells. Conclusion: Pachouli alcohol can inhibit the proliferation of DU145 in vitro, which may be correlated with inducing the cell apoptosis.关键词：pachouli alcohol;human prostate cancer cell line DU145;proliferation;cytochrome C pathway;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To observe the inhibitory effects of different extractive fractions from Camellia chrysantha on five different kinds of malignant tumor cell lines, and evaluate the antitumor effects in vitro and its material basis of the effects. Method: MTT assay was adopted to detect the inhabitation of different extractive fractions from C. chrysantha with five different kinds of malignant tumor cell lines in vitro and IC50 values were calculated,respectively. The UPLC-LTQ-Orbitrap MS/MS was used to analyse its possible components. Result: The different extractive fractions from C. chrysantha had antitumor effects on SGC-7901, H460, SMMC-7721, BEL-7404, CNE-1,they inhibited cell proliferation in a dose dependent manner. The sequence of their inhabitation intensity was:water-soluble part>n-butyl alcohol part>ethyl acetate part, and the IC50 of water-soluble part on the five different kinds cell lines was 81.72,73.47,95.98,73.41,61.25 mg·L-1,respectively;24 components were investigated, 11 of them were tentatively identified, and 13 of them were characterized by their mass spectra information. Conclusion: Different extractive fractions from C. chrysantha can inhibit the proliferation of SMMC-7721, BEL-7404, CNE-1, SGC-7901 and H460 in vitro,the water-soluble part is considered as antitumor active part of C. chrysantha;but which component exactly contribute to the antitumor effect remains to be further confirmed.关键词：Camellia chrysantha;antitumor in vitro;UPLC-LTQ-Orbitrap MS/MS assay;material basis of effects
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发布时间：2024-01-04 - 摘要：Objective: To establish the scald model on skins of two sides of buttock in mice by punctate-like scald device that we invested. To compare the therapeutic effects of different yolk oils which prepared by fried fresh yolk based on the model we established. Method: Scald model was established. Healthy Kunming mice, half male and half female, were used in the model. The mice were divided into two groups, high weight group(33.44±3.97) g and low weight group (27.42±1.66) g. The mice were fixed by adhesive bands. A 1.5 cm-diameter circle scalded area was made on each side of buttock of the mice by punctate-like scald device. The time that the scalded area of the mice began to scab at first and the skin samples of scalded area of the mice were collected and analyzed. The effect on the model we established by the time that the scald device covered the skin and the weight of the mice were explored. The efficacy of different drugs were compared. After the mold was built, the mice were randomly divided into 14 groups, namely model control group, positive control group that treated with silver sulfadiazine cream, egg yolk oil treatment group, duck yolk oil treatment group concluding, egg yolk oil and duck yolk treatment group, egg oil and silver sulfadiazine cream treatment group, yolk oil and silver sulfadiazine cream treatment group. Each group all divided into male group and female group. Healing times of scald wound of each group were recorded. The effect of the drugs were compared according to the data we obtained. Result: There are successful models in the groups, concluding the group which contacting time was 5 s with two weights of mice, contacting time was 10 s with high weight of mice. The mice were all died in the groups of contacting time was 10 s with low weight of mice and contacting time was 25 s of two weights of mice. The time that the scalded area of the mice began to scab at first and degree of injure were affected by the weight of mice. In 5 s group, the time was significant different between the high weight and low weight(P<0.01). In the male mice group, healing time was reduced significantly in positive control group than in model control group(P<0.01). There was no significant difference between positive control group and egg-duck yolk treatment group(P>0.05). In this experiment, the healing effect of duck yolk oil was not as good as egg yolk oil in this test(P<0.01), female mice showed a stronger self-healing ability(P<0.01) than male mice. Healing effect of duck yolk oil has significant sexual difference(P<0.01),while the egg yolk oil has no such cases(P>0.05). Conclusion: The skin scald model has character of good replicability because the punctate-like scald device we invested in this experiment is easy to control the scald temperature. The duck yolk oil has significant sexual difference(P<0.01) in healing wound and its effect is less than the egg yolk oil.关键词：punctate-like scald device;two sides skin scald on the buttock;deep Ⅱ degree;Yolk oil distillation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) on Dectin-1, Syk and CARD9 mRNA expression of dendritic cells. Method: Dendritic cells derived from bone marrow of ICR mice were cultured in vitro; the expression of CD11c and CD86 molecule on the surface of dendritic cells was detected by flow cytometry; the effect of LMPAB on the proliferation of T lymphocytes stimulated by dendritic cells was detected by WST. Real-time quantitative PCR detect Dectin-1 mRNA, Sky mRNA and CARD9 mRNA expression levels. Result: CD11c and CD86 protein expression were expressed by 98.6% and 96.97% respectively; compared with the control group (4.84±0.21), LMPAB+OVA group (6.59±0.19) enhances the effect of dendritic cells on stimulating T lymphocytes' proliferation (P<0.05). Compared with the mRNA expression of Dectin-1 (6.13±0.27),Sky (0.36±0.11) and CARD9(1.85±0.20) in the control group, Dectin-1 (8.25±0.19), Sky (1.63±0.16) and CARD9 (4.40±0.19) mRNA expression in LMPAB+OVA group was significantly increased with statistically significant (P<0.05). Conclusion: The cells derived from bone marrow were dendritic cells; immunizing potency could be enhanced by LMPAB regulating and controlling Dectin-1, Sky, CARD9 mRNA expression levels in Dendritic cells.关键词：LMPAB;Dendritic cell;Dectin-1;Syk;CARD9
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发布时间：2024-01-04 - 摘要：Objective: To study Qili Qiangxin capsule(QL)' s function in improves the chronic heart failure rats' cardiac function and its' influence in the aquaporin-2 Water channel expression, then discuss benefit of how Qili Qiangxin therapies for disorders that chronic heart failure rats' aberrant water movement. Method: The proximal left anterior descending branch (LAD) of coronary artery was ligated using a terylene suture in acute myocardial infarction(AMI) rats, after building AMI model successfully , survivors were randomly divided into four groups, model group (equal volume of water), QL group (1 g·kg-1·d-1), valsartan group (10 mg-1·kg-1·d-1), sham-operated group (equal volume of water) .Drug intervention four weeks ,then detection of related indicators. Echocardiography and the expressions of aquaporin-2 were detected by the method of immunohistochemistry and western blotting were assessed 4 weeks after the drug therapy. Result: Compared with the sham-operated group, the left ventricular end-diastolic dimension(LVEDD) and left ventricular end-systolic dimension(LVESD) of model group is significantly increased, ejection fractions(EF) and factional shortening(FS) was significantly lower (P<0.05); compared with model group, QL group LVEDD and LVESD is significantly reduced , EF and FS were significantly higher (P<0.05). There was no significant difference between QL group and valsartan group; compared with the sham-operated group , the aquaporin-2 water channel expression levels were significantly higher in model group(P<0.05); compared with the model group, the AQP-2 expression were reduced to varying degrees in QL and valsartan group (P<0.05); while there was no significant difference between QL group and valsartan group. Conclusion: QL can be effectively reduced AQP-2 expression levels, improve chronic heart failure caused by the water metabolism, elimination of edema symptoms, thereby improving the quality of life in patients with chronic heart failure.关键词：chronic heart failure;Qili Qiangxin capsule;aquaporin-2
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发布时间：2024-01-04 - 摘要：Objective: Biyuan Tongqiao granule for chronic sinusitis in children clinical effect mechanism of action. Method: One hundred and twenty patients were randomly divided into control group (60 cases) and observation group (60 cases).Patients the control group were treated with amoxicillin and clavulanate potassium suspension,4-7 years old,1 sack/time,2 times/day,7-12 years old, 1.5 sack/time,2 times/day.The treatment course of the group was 4 weeks. Ambroxol granule,1.2-1.6 mg·kg-1,taken orally by 2-3 times.The treatment course of the group was 6 weeks. Standard myrtol standardized enteric coated soft capsules(children pack),120 mg/time,3 times/day.The treatment course of the group was 6 weeks.The observation group was added Biyuan Tongqiao granule based on the control group,5-10 g/time,3 times/day.The treatment course of the group was 6 weeks. Determining nasal mucosa transport time(MTT).Detection of serum interleukin-2(IL-2) and interleukin-6(IL-6),with endoscopy,the results were evaluated by Lund-Kennedy score method,recordding main symptoms and sign grade before and after treatment. Result: The total effective rate of the observation group was 95% superior to 81.7% of the control group(P<0.05),after treatment,the level of IL-2 and IL-6 in the observation group were lower than those in the control group(P<0.01), after 3 months of treatment,MTT and Lund-Kennedy score in the observation group were lower than those in the control group(P<0.01),For the observation group, the symptom such as nasal obstruction,streamed pus nasal discharge,headache, back to the sputum suction and hyperemia swelling of nasal mucosa symptoms and sign grades were all lower than those in the control group(P<0.01). Conclusion: Biyuan Tongqiao granule was used to chronic sinusitis in children patients, It can inhibit the proinflammatory factor and promote the nasal mucosa cilia function, improve the clinical symptoms of patients and sign when when,Have certain curative effect in clinic.关键词：chronic sinusitis;Biyuan Tongqiao granule;nasal mucosa cilia function;interleukin-2;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to observe recent curative effect of Yuyang Xiaokui decoction in the treatment of ulcerative colitis (UC) active stage and its influence on the leukocyte betweenness-17, 21 (IL-17, IL-21). Method: Ninety cases of patients with UC were randomly divided into control group (45 cases) and observation group (45 cases) according to numeric method. The patients in control group took the mesalazin enteric-coated tablets, to swallow not chew, 1 g/time, 4 times/day. For patients in observation group,they took Yuyang Xiaokui I decoction on the basis of the control group, 1 dose/day, conventional water decoction, orally, 2 times;Yuyang Xiaokui Ⅱ decoction, 1 dose/day, severe times boiling concentration to 120 mL, enema before sleeping, continue 5 days before 2 days suspend. The treatment course was 8 weeks. The Sutherland disease activity index (Sutherland DAI) was recorded before and after treatment and the response rate was calculated. The colonoscopy expression and colon tissue pathological changes were observed. The main symptoms, signs score were recorded before and after the treatment and the level of serum IL-17, IL-21 was detected. Result: After treatment, the Sutherland DAI, colonoscopy performance score and intestinal tissue pathological change score of two groups were decreased (P<0.01), while the related scores of observation group were all lower than the control group (P<0.01). The diarrhea, purulent blood, abdominal pain, abdominal distension, anus hot, anal congestion edema and erosion rate were decreased (P<0.01), while the main symptoms and related scores of observation group were all lower than the control group (P<0.01). There are 31 cases remission in observation group and 20 cases remission in control group with remission rate 68.9% and 44.4%, respectively. The observation group is better than that of control group (P<0.01). The levels of serum IL-17, IL-21 of two groups were decreased after treatment (P<0.01), while the level of serum IL-17, IL-21 of observation group is lower than the control group (P<0.01). Conclusion: The oral taking and enema of Yuyang Xiaokui decoction can reduce degree of disease activity of patients with UC in light and moderate degree of active stage, alleviate clinical symptoms and sign of patients with UC and promote it into remission. Its mechanism may be related to reduce inflammatory reaction that mediated by Th17 cells.关键词：ulcerative colitis;active stage;Yuyang Xiaokui decoction;leukocyte betweenness-17;leukocyte betweenness-21
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发布时间：2024-01-04 - 摘要：Objective: Discussing curative effect of Xuesaitong injection on acute cerebral, the influence to thrombosis and mechanism of action. Method: Sixty three cases with acute cerebral infarction randomly divided into control group (30 cases) and observation group (33 cases).Both groups' patients received medical treatment refer to 'Chinese Cerebral Ischemic Stroke Treatment Guidelines in 2010'.Anticoagulant use worship aspirin enteric-coated tablets,100 mg/time,1 time/day, taken orally after dinner.Based on treatment of the control group,patients in the observation group added Xuesaitong injection,0.4 g/time,intravenous drip,1 time/day.Course of treatment in two groups was 14 days. Severity degree of neurologic impairment by national institutes of health stroke scale (NIHSS). In 7, the 14 day evaluation,detecting the change of antithrombase (ATⅢ),fibrinogen(FIB),given D-dimer(D-D),blood platelet count,platelet volume distribution width (PDW),mean platelet volume (MPV),large platelet ratio (P-LCR),platelet aggregation rate (MPAR),thromboxance B2(TXB2) and 6-keto-prostaglandin F_la (6-Keto-PGF_la) before and after treatment. Result: Analyzed by ridit,clinical curative effect in the observation group was superior to the control group(P<0.05).At the seventh day after treatment,NIHSS scores in the two groups decreased(P<0.01).At the fourteenth day after treatment,NIHSS scores in the two groups sequentially decreased(P<0.01),and NIHSS score in the observation group was lower than the control group(P<0.01).After treatment, FIB,D-D and MPAR in the observation group were all lower than the control group(P<0.01),ATⅢ was higher than the control group(P<0.01).Compared with the time before treatment,PDW,MPV and P-LCR in the observation group reduced,and compared with the control group after treatment,which also meant a statistical significance from the differences between two groups(P<0.05 or P<0.01).The level of TXB2 in the observation group was lower than the control group,and 6-Keto-PGF_la was higher than the control group(P<0.01). Conclusion: Xuesaitong injection can antiplatelet aggregative,restrain forming thrombosis by several routes,ameliorated acute cerebral infarction region and blood supplying of penumbra around,and improve the recovery of nerve function.关键词：acute cerebral infarction;Xuesaitong injection;platelet aggregation rate;platelet volume
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to observe curative effect of Xingshen Tongluo needle medicin improve ischemic stroke at recovery phase on neurologic impairment and its influence on cerebral blood flow speed and vascular endothelial function. Method: Eighty-four cases patients were randomly divided into control group (42 cases) and observation group (42 cases) according to digital method.Both groups were received secondary prevention plan ischemic brain death conventional cure in secondary prevention plan, including anti-platelet aggregation, blood sugar control,blood pressure control,regulating blood lipids et.Observation group took Xingshen Tongluo decoction on the basis of therapy in control group,1 does/day,conventional water frying.At the same time adopt Xingshen Tongluo acupuncture,3 times/week.Treatment courses for the two groups continued 8 weeks.Before the treatment,give a mark of nervous functional defects degree with stroke patient and record activities of daily living (ADL).Measuring arteria cerebri anterior(ACA) through transcranial doppler instrument's treatment,middle cerebral artery(MCA),posterior cerebral artery(PCA)and basilar artery(BA) blood flow rate in cerebral infarction side.Detect the level of nitric oxide(NO) and endothelins1(ET-1). Result: The total effective rate of disease curative effect in the observation group was 83.33% superior to 57.14% of the control group(P<0.05).After treatment,except consciousness factor,in the observation group,the score of the degree of neural function defect score standard(CSS) and the total score of CSS were lower than those in the control group(P<0.01).And the score of ADL was higher than that of the control group(P<0.01), after treatment, patients' illness in both group were improved.Besides blood velocity of ACA and MCA in observation group were better than those in the control group(P<0.01).For the two groups,compared with the time before treatment,blood velocity of PCA both improved(P<0.01).After treatment the level of NO in the two groups both increased, observation group which higher than that of the control group(P<0.01). And endothelins1 (ET-1) in the two groups both decreased,and for the observation group which lower than that of the control group(P<0.01). Conclusion: For ischemic type stroke patients, it can improve blood velocity of ACA and MCA Xingshen Tongluo needle medicine with drug was used in convalescence,and it improved function of vascular endothelium,cerebral blood perfusion,Promote the recovery of neural function.关键词：ischemic stroke;Xingshen Tongluo decoction;Xingshen Tongluo acupuncture;vascular endothelial function;haemodynamics
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发布时间：2024-01-04 - 摘要：Objective: To investigate the curative effects of Huangkui capsule on dampness-heat of chronic kidney disease (CKD) 3 phase. Method: The patients were randomly divided into conventional western medicine treated group (control group, 36 cases) and conventional western medicine plus Huangkui capsule treated group (treatment group,37 cases).The serum-creatinine (SCr),blood urea nitrogen (BUN),proteinuria (Pro) and glomerular filtration rate (GFR)were examined in 37 cases, compared with conventional medicine treated group with western medicine. Result: Combined treatment of Chinese and western medicine can improve symptoms and decrease SCr,proteinuria,and increase the level of GFR in the patients with dampness-heat of CKD 3 period patients. Conclusion: Huangkui capsule combined with conventional western medicine can reduce proteinuria and improve renal function, can effectively increased significantly the GFR in the patients with CKD 3 period of chronic nephritis.关键词：Huangkui capsule;dampness-heat;chronic nephritis;chronic kidney disease 3 period
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of traditional Chinese medicine(TCM) compound on high-sensitivity C-reactive protein,interleukin-6 in patients with diabetic angioplasties. Method: Seventy-four patients with diabetic angioplasties(DA) were randomly divided into treatment group and control group. All patients were treated with basic treatment of diabetes. Treatment group was treated with, basic treatment plus TCM decoction ginseng-astragalus compound for 8 weeks;control group was treated with the basic treatment of diabetes.The patients' symptoms, signs, C-reactive protein,interleukin-6 before and after treatment were observed. And comprehensive efficacies were evaluated. Result: Treatment group could significantly reduce the levels of C-reactive protein,interleukin-6.The difference of two groups was statistically significant (P<0.05).Treatment group significantly improved symptoms, signs, blood glucose, blood lipid and glycated hemoglobin of the patients. Conclusion: The method of boosting qi and nourishing yin, transforming stasis and freeing the collaterals can significantly improve the clinical symptoms, reduce C-reactive protein, interleukin-6, effectively improve the status and glucolipid metabolism, and can prevent the occurrence and development of DA.关键词：Boosting Qi and nourishing Yin;diabetes vascular lesions;high-sensitivity C-reactive protein;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: To investigate clinical efficacy of traditional Chinese medicine(TCM) in treatment of chronic obstructive pulmonary disease combined with respiratory failure. Method: Ninty patients with chronic obstructive pulmonary disease with respiratory failure were chosen from January 2009 to December 2012 in our hospital and randomly divided into two groups including control group (45 patients) with conventional symptomatic treatment of western medicine and TCM treatment group (45 patients) with TCM differentiation treatment on the basis of control group treatment;and the total efficiency of clinical improvement, clinical mortality, before and after treatment TCM syndrome score, APACHE Ⅱ score and blood gas analysis index of both groups were compared. Result: The total efficiency of clinical improvement of TCM treatment group (93.3%)was significant higher than control group (71.1%)(P<0.05). The TCM syndrome score, APACHE Ⅱ score and blood gas analysis index of both groups after treatment were significant better than before treatment and the improving degree of TCM treatment group was significant higher than control group(P<0.05). Conclusion: TCM treatment of chronic obstructive pulmonary disease with respiratory failure combined with western medicine can efficiently relieve dyspnea, and higher pulmonary ventilation function.关键词：differentiation treatment;chronic obstructive pulmonary disease;respiratory failure
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study is to observe the curative effect of Jiawei Buyang Huanwu decoction in treating degenerative spondylolisthesis. And to compare whether it is superior to the traditional treatment method combined with manipulation in improving the lumbosacral angle or enhancing the stability of lumbar spine. Method: One hundred and forty-two cases of degenerative lumbar spondylolisthesis patients were randomly divided into experimental group of 68 cases and 74 cases in the control group, experimental group was treated with Jiawei Buyang Huanwu decoction combined with manipulation, while the control group was given celecoxib capsules 200 mg 1 times a day orally combined with manipulation, 2 weeks for 1 courses. Before treatment and after 1 courses of treatment, the patients' symptoms were scored by JOA and the lumbosacral angle was measured by Ferguson method. The rate of improvement was calculated according to the changes in the score and lumbosacral angle before and after treatment. Result: The total effective rate of experimental group was 95.59%, while the rate of the control group was 90.54%, there was significant difference between the two groups (P<0.05). Lumbosacral angle in the experimental group before treatment was (46.45±1.21)°, after treatment, the lumbosacral angle was (41.24±1.14)°.However, in the control group, lumbosacral angle was (46.29±1.18)° before treatment and (43.17±1.01)° after treatment. There was significant difference between two groups (P<0.05). Conclusion: Jiawei Buyang Huanwu decoction has good curative effect in treating degenerative lumbar spondylolisthesis,and it is superior to the traditional treatment method combined with manipulation in improving the lumbosacral angle and enhancing the stability of lumbar spine.It is a safe and effective treatment with less adverse reactions in treating the degenerative lumbar spondylolisthesis.关键词：Buyang Huanwu Decoction;manipulation;degenerative lumbar spondylolisthesis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Tanreqing injection in treatment of acute pneumonia (syndrome of phlegm-heat obstructing lung). Method: The acute pneumonia February were (syndrome of phlegm-heat obstructing lung) patients were randomly divided into study group and control group. Patients of two groups were given correct electrolyte disorders, sputum suction, antispasmodic asthma, leukotriene stabilizer, controling blood glucose, blood pressure, heart failure, lipid and other symptomatic treatment. Patients of the control group were given Shuanghuanglian injection 20 mL in 5% glucose injection 500 mL based on above conventional treatment, intravenous drip, one time per day. The treatment group was given Tanreqing injection 20 mL in 5% glucose injection 500 mL by intravenous drip based on above conventional treatment, one time per day. 2 weeks were as a course of treatment. Clinical efficacy and adverse reactions were compared between two groups. Result: 2 weeks after treatment, patients in the study group the total efficiency (90.63%) significantly higher than that in control group (68.75%), and the difference was statistically significant (P<0.05). Heating time and eliminate time in the study group were significantly shorter than that in the control group [(41.35±21.32)h, (70.71±33.65)h] were significantly shorter than that in the control group[(50.85±28.33)h,(80.36±41.03)h], and the difference was statistically significant (P<0.05). Patients in two groups during treatment had no hearing loss, acute kidney damage;there were 1 cases of treatment of electrocardiograph(ECG) after prolonged QT interval in patients of the study group, while patients in the control group without any adverse reactions, by statistical analysis of the incidence of adverse reaction in group with no significant difference compared to control group. Conclusion: Tanreqing injection in the treatment of acute pneumonia (syndrome of phlegm-heat obstructing lung) has clinical curative effect, less adverse reaction.关键词：Tanreqing injection;cute pneumonia;syndrome of phlegm-heat obstructing lung
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发布时间：2024-01-04 - 摘要：Objective: To observa the clinical efficiency of ntervention in recovery process of general anesthesia by combination of traditional Chinese medicine(TCM) and acupucture. Method: One hundred and twenty patients of general anesthesia abdominal surgery (TCM syndrome:Yang deficiency,60 cases;Yin deficiency,60 cases) were randomized into treatment and control groups. Each type of treatment group and control group got 30 cases. Anesthesia was induced and maintained in the same way, and after the end of surgery medicines were administered intravenously. Infusion of normal saline was for control group.Treatment group was given electric acupuncture,and type Shenfu injection or in;travenous infusion of silent myocardial ischemia(SMI). Outcome measures:blood pressure (SBP/DBP), heart rate (HR), body temperature, anesthesia recovery time, nausea, vomiting incidence. Result: Treatment group, compared with the control group, the recovery time for blood pressure (SBP/DBP), and HR was shorter(P<0.05);the temperature did not change significantly;anesthesia recovery time was shortened (P<0.05);the incidence of nausea and vomiting was reduced(P<0.05). Conclusion: For the recovery of of surgical patients after general anesthesia,TCM acupuncture is feasible.TCM treatment can effectively shorten the patients' recovery time, maintain loop stability and reduce complications.关键词：anesthesia recovery;Yang deficiency;Yin deficiency;acupuncture plus medication
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effects of Yupingfeng decoction combined with moxibustion for cough variant asthma. Method: Fourty-four cases were randomly divided into 2 groups on average,22 were Yupingfeng decoction combined with moxibustion group(YM), 22 control group with Montelukast sodium tablets. Their therapeutic effects were assessed by clinical symptoms, relapse cases and the changes of lung function. Result: The total effective rate, relapse cases and the changes of lung function of YM group were significantly superior than those of control group(P<0.01). Conclusion: Yupingfeng decoction combined with moxibustion is an effective method for cough variant asthma and is valued to be popularized.关键词：Yupingfeng decoction;moxibustion;cough variant asthma
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Danshen and Huangqi injection in treatment of chronic heart failure. Method: Eighty-four patients were randomly divided into two groups. The treatment group and the control group were treated with the same routine medicine, while the treated group was additionally treated with Danshen and Huangqi. The clinical efficacy before and after treatment and the changes of color doppler ultrasonic cardiogram examinations were both observed. And walking distance in 6 minutes was detected. Result: The apparent efficiency, the total effective rate and walking distance in 6 minutes in the treated group were remarkably better than that in the control group(P<0.05). The walking distance in 6 minutes and the rate of left ventricle eject fraction (LVEF), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), blood pressure and heart rate of the two groups were obviously improved after treatment (P<0.05). Each indexes in the treatment group were improved more than that of the control group (P<0.05). Conclusion: Danshen and Huangqi injection is an effective and safe treatment with less side effect.关键词：chronic heart failure;Salvia Miltionrrhiza injection;Astragalus injection;integrative medicine therapy
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the Aihuo Niaotong pills combined with Bazheng san add-subtract clinical curative effect of the treatment of damp heat typeprostatitis. Method: The patients with damp heat type chronic prostatitis were studied by method of random, the control group, the treatment group 82 cases and control group 82 were statistically analyzed. The control group for the Aihuo Niaotong pills group, to observe the clinical curative effect of Aihuo Niaotong pills combined with Bazheng san add-subtract. Result: Statistically significant between the two groups before and after treatment of symptom score and NIH-CPSI score difference comparison, the control group Conclusion: Treatment group was better than the Aihuo Niaotong pills combined with Bazheng san add-subtract positive powder decoction treatment, can significantly improve the damp heat type chronic prostatitis symptom score and NIH-CPSI score, has good clinical efficacy.关键词：turbid sperm;damp heat syndrome;chronic prostatitis;Bazheng san add-subtract decoction;Aihuo Niaotong pills
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发布时间：2024-01-04 - 摘要：Objective: To observe clinical curative effect of combined therapy of traditional Chinese and western medicine formed in treating the cerebral thrombosis, and to evaluate its clinical application effect, providing a reference for clinical treatment in the future. Method: One hundred and twenty cases of patients with formed cerebral thrombosis admitted by this hospital from August 2012 to August 2012 were randomly selected in this research, patients selected were divided into three groups including group A, group B and group C by random number method, patients in group A were subject to western medicine treatment, patients in group B were subject to the therapy of traditional Chinese medicine, and patients in group C were subject to combine therapy of traditional Chinese and western medicine, therapeutic effects on patients in three groups were compared and analyzed. Result: The total effective rate in group C hit 97.50%, being significantly higher than which of group A with 70.00% and that of group B 72.50%(P<0.05).The Fugl-meyer score and Barthel index score of group C after treatment were significantly higher than that both group A and group B (P<0.05), the difference was statistically significant. Conclusion: The combined therapy of traditional Chinese and western medicine in treating patients with cerebral thrombosis can effectively improve clinical symptoms of patients, restore their movement function and improve their ability of daily life, the curative effect is distinct and of high safety, hence it is worth wide application and promotion in clinical practices.关键词：combine traditional Chinese and western medicine;cerebral thrombosis;clinical effect
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发布时间：2024-01-04 - 摘要：The common carrier material and new carrier material were summarized in past dacades. Common materials include protide, polysaccharide, polyester, polyethylene glycol and so on. The new carrier materials include PACA-co-PEG, star polymer for drug delivery application and modified chitosan.Some problems of making microsphere were summarized in recent years. Microsphere has the function of controlled-release and targeting. The technology of microsphere in the field of medicine has been widely studied and developed, but the technology of microsphere in field of traditional Chinese medicine (TCM) lies in basic research stage. The development of this technology in the field of TCM preparation in future was prospected. Microsphere of TCM improves the shortage of traditional dosage form which can enlarge the clinical application of TCM.关键词：microsphere;common carrier material;new carrier material
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