最新刊期
卷 17 ， 期 21 ， 2011
- 摘要：Objective: To explore influence discipline of chitosan flocculation clarification and alcohol precipitation for aqueous solution of Yiganning compound. Method: Sample was investigated by multi-indexes to evaluate difference of two purification methods for aqueous solution of Yiganning compound, such as yield of extracts, physic liquor clarity, the content of carbohydrate(polyose, monosaccharide, oligosaccharide) and three active component(peoniflorin,salvianolic acid B and paeonol), moisture absorption ratio. Result: Yield of extracts of ethanol precipitation samples was close to chitosan clarification samples.Physic liquor clarity,transfer rate of carbohydrate and paeoniflorin of ethanol precipitation samples was lower than chitosan clarification samples,while transfer rate of salvianolic acid B and paeonol of ethanol precipitation samples are higher than chitosan clarification samples.Extracts hygroscopicity of ethanol precipitation samples are higher than chitosan clarification samples. Conclusion: The result provided a feasible method for investigating purification process of herbal extract preparation, and could be used for purification preparation craft optimization of herbal praeparatum.关键词：Yiganning;chitosan;flocculation clarification;alcohol precipitation;hygroscopicity
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发布时间：2024-01-04 - 摘要：Objective: To optimize ethanol extraction technology of sinapine effective-part in roasted Semen Raphani and to determine the content of sinapine in Semen Raphani bought from ten different areas. Method: Optimum extraction process was established with L9(34) orthogonal design. The content of sinapine and extraction rate of extraction were chosen as indexes.The conent of sinapine in commercial samples was determined by HPLC. Result: Optimum extraction process was as follows:25 times of 75% alcohol,extracted 1 time for 60 minutes. The content of sinapine was different between different samples of roasted Semen Raphani. Conclusion: Optimized alcohol extraction process of sinapine effective-part from roasted Semen Raphani was stable and feasible.Some suggestion is raised to 〈China Pharmacopeia〉 for the determination of sinapine by HPLC.关键词：roasted Semen Raphani;orthoponal test;sinapine;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study on preparation process of As2O3 magnetic microspheres. Method: To prepare Fe3O4 magnetic particles by chemical coprecipitation, prepared As2O3 magnetic microspheres by phacoemulsification and solvent extraction evaporation method, established a method to determinate encapsulation efficiency of microspheres by DDC-Ag method and to optimize preparation technology. Result: Optimum process was A3B2 C 1 ,Fe3O4-As2O3(1 ∶2), concentration of polylactic acid was 0.6% and concentration of PVA was 3%. The average content of prepared magnetic microspheres was 26%, encapsulation efficiency was 60%. Conclusion: The prepared As2O3 magnetic microspheres had high encapsulation efficiency with less toxicity.关键词：As2O3;magnetic microspheres;preparation process;encapsulation efficiency
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发布时间：2024-01-04 - 摘要：Objective: To determine equilibrium solubility of Ganoderma triterpenes and its apparent partition coefficients for n-octanol-water/buffer solution systems, and to verify its absorption by in situ perfused rat intestinal model. Method: Ganoderma triterpenes were divided into acidic and neutral triterpenoids. Visible spectrophotometry was established and used to detect their concentration in different pH solvents,and also partition coefficients. The elimination of the percentage of several components in different intestinal segments presume intestinal absorption of triterpenes. Result: The equilibrium solubility of acidic and neutral triterpenoids were less than 100 mg·L-1 in water at 37 ℃, log P were 1.83 and 1.52 respectively. Their oral intestinal absorption were both well, neutral triterpenoids wererelatively better. Conclusion: Ganoderma acidic and neutral triterpenoids both had poor water-solubility, their dissolution was the rate-limiting process of their absorption.关键词：Ganoderma triterpenes;apparent oil/water partition coefficient;in situ intestinal perfusion
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of hydroxypropyl-β-cyclodextrin on solubility of ginsenoside Rg1. Method: The content of ginsenoside Rg1 was determined by HPLC,orthogonal test was used to study on concentration of hydroxypropyl-β-cyclodextrin、temperature and stirring time,single factor test was used to analysis the influence of pH to solubility of ginsenoside Rg1,and investigated freeze-thaw stability of samples prepared in these conditions; Result: Optimum conditions for hydroxypropyl-β-cyclodextrin improving solubility of ginsenoside Rg1 were as follows:concentration of hydroxypropyl-β-cyclodextrin 40%,temperature 40 ℃,stirring time 12 h.When pH was 7,solubility of ginsenoside Rg1 was 353.20 g·L-1. Freeze-thaw stability of samples was good. Conclusion: Solubility of ginsenoside Rg1 had greatly improved by using hydroxypropyl-β-cyclodextrin.关键词：hydroxypropyl-β-cyclodextrin;Ginsenoside Rg1;solubility;freeze-thaw
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of traditional Chinese medicine adhesive plaste. Method: To investigate common technology of traditional Chinese medicine adhesive plaste in preparation process such as safty and comfortable,using single-factor test to investigate solvent which could cause allergy,employing peel strength,softness and paste uniformity as index,using orthogonal test to investigate factors which could influence paste properties as solvent, making gum time and the adding order of each bland matrix part,then optimized condition. Result: The best condition in preparation process was: n-hexane as solvent with 50 mL;2 h as making gum time; firstly,rubber homogenate mixed with softener,then adding viscous agent and filling agent. Conclusion: Optimized owning low allergy rate and high comfortable.关键词：traditional Chinese medicine adhesive plaste;preparation process;safty;comfortable
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发布时间：2024-01-04 - 摘要：Objective: To study on transfer rate of chlorgenic acid in preparation process of Yinqiao Jiedu soft capsules, and to provide experimental data for preparation process optimization and quality control. Method: According to prescription and technology of Yinqiao Jiedu soft capsules contained in Pharmacopoeia, transfer rate and retention rate of chlorgenic acid in preparation process of Yinqiao Jiedu soft capsules were determined by HPLC. Meanwhile, the content of chlorgenic acid in 3 batches of Yinqiao Jiedu soft capsules were determined by HPLC. Result: Preparation process contained 4 steps: ethanol refluxing, decompression concentration, addition of other herbs’ thickpaste and desiccation, addition of naphtha and bean oil. The mean transfer rate of chlorgenic acid in above steps were 86.1%, 77.5%, 73.2% and 72.8%;and mean retention rate were 86.1%,90.0%,94.6% and 99.3% respectively. The content of chlorgenic acid in per capsule of Yinqiao Jiedu soft capsules with three batches were 3.77, 3.38, 3.86 mg. Conclusion: This study could provide reference for quality control and content limit standard establishment in preparation process of Yinqiao Jiedu soft capsules.关键词：Yinqiao Jiedu soft capsules;Lonicera japonica;chlorgenic acid;transfer rate;retention rate
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发布时间：2024-01-04 - 摘要：Objective: To optimize matrix formulation process of Xiaoguzeng patch. Method: L18(37) orthogonal test was designed, NP700, glycerol,EDTA,PVPPXL-10,aluminum glycinate,PVPK90 and liquid medicine as factor, dosage of these as lever. Adhesion force,membrane residues and composite indicator were considered as index. Result: The greatest impact on adhesion force was NP700, followed by glycerol, and they had a significant effect on adhesion force. The greatest impact on membrane residues and composite indicator was PVPPXL-10.Optimum matrix formulation of Xiaoguzeng patch was NP700-glycerol-EDTA-PVPPXL-10-aluminum glycinate-PVPK90-liquid medicine as 6 ∶30 ∶0.025 ∶1 ∶0.05 ∶1 ∶20. Conclusion: This process was simple and easy to industrialization.Prepared by optimum matrix formulation,gel ointment matrix shows good viscosity viscidity and lower membrane residues,smooth appearance, conformed to medicinal standard.关键词：Xiaoguzeng patch;gel ointment;matrix;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To investigate release discipline of paeoniflorin in different processed Paeonia lactiflora in boiling process. Method: P. lactiflora processed products were boiled alone.HPLC was used to determine the content of paeoniflorin in boiling liquid and decoction pieces. Result: The content of paeoniflorin in P. lactiflora decreased after processed. P. lactiflora carbon and baked P. lactiflora decreased the lowest, release speed in baked P. lactiflora was the highest during 30 min.The content of paeoniflorin in P. lactiflora and baked P. lactiflora was the highest after boiled. Conclusion: Processing made paeoniflorin declined in all processed products, declined most significant were P. lactiflora and baked P. lactiflora ; but it could increase release speed,especial in baked P. lactiflora .关键词：HPLC;paeoniflorin;processed;Paeonia lactiflora;release
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发布时间：2024-01-04 - 摘要：Objective: To study on optimum extraction technology for total flavonoids from fruits of Physalis alkekengi. Method: Based on single factor test,investigated influence of four factors to extract of total flavonoids from P. alkekengi by orthogonal design test.The four factors were concentration of ethanol、ratio of solid to liquid、extraction time and extraction times.Got optimal extraction technology by determined the content of total flavonoids from fruits of P. alkekengi UV-visible spectrophotometer. Result: The optimum extraction technology was as follows:extracted 3 times wtih 30 times amount of 60% ethanol for 30 minutes each times. The average extract of total flavonoids was 2.00 mg·g-1. Conclusion: Total flavonoids from fruits of P. alkekengi can be extracted with ethanol by ultrasonic wave.Ratio of liquid to solid was the most significant factor.关键词：Physalis alkekengi;total flavonoids;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation of Arnebiae Radix ointment. Method: An in vitro model of transdermal permeation was established. In vitro penetration rate of Arnebiae Radix ointment was examined by HPLC, permeation curve was fitted to the Higuchi equation. Result: O/W ointment of Arnebiae Radix had the best penetration rate in all the five formulations,average J value was up to 14.797 μg·h-1·cm-2 calculated with shikonin. Besides, its cumulative permeation curve fitted to the Higuchi equation best. Conclusion: Optimize formulation was O/W ointment.关键词：Arnebiae Radix;ointment;shikonin;transdermal experiment in vitro;HPLC
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发布时间：2024-01-04 - 摘要：Objective: In order to develop and use the polysaccharide in Lycopus lucidus. Method: In this paper, the hot extraction method was chosen to extract the water-soluble polysaccharides from L.lucidus. Orthogonal test and single factor test were respectively performed on the solid- liquid ratio, temperature, time of the extraction, and times of the extraction. Result: The best technological conditions were as follows: the temperature of extraction was 80 ℃, the ratio of water to sample was 15 ∶1, and the time of extraction was 3 hours, extracting 1 time. Conclusion: The method is easy to operate, rapid to extract, and able to obtain the high content of polysaccharide And under the extraction conditions, the yield of polysaccharides was up to 16.8%.关键词：Lycopus lucidus polysaccharide;orthogonal test;Extracting Technology
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发布时间：2024-01-04 - 摘要：Objective: To establish a method of scavenging DPPH radical, and then evaluate DPPH radical scavenging effect of compound Tangmaikang. Method: Investigated the reaction time, temperature, the concentration of DPPH and the extraction solvent and method, the study on the DPPH radical scavenging effect of 14 batches Tangmaikang and drug-containing serum was carried out. Result: Tangmaikang was extracted with 60% methanol by ultrasonic for 30 min. The optimum reaction conditions were obtained as follows:DPPH concentration 0.099 8 g·L-1, reaction temperature 60 ℃, reaction time 50 min. The IC50 of 14 batches of Tangmaikang was 0.48 mg. The DPPH clearance of drug-containing serum was lower than blank serum. Conclusion: Tangmaikang has antioxidant activity in vitro.关键词：Tangmaikang;DPPH;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: To establish a microwave-assisted extraction(MAE)method for the extraction of the baicalin from Scutellaria baicalensis. Comparison of the determination of baicalin from S. baicalensis between MAE and Chinese pharmacopeia method. Method: Dikma Diamonsil C18 column(4.6 mm×150 mm,5 μm)was used. The mobile phase of methanol-water-phosphoric acid (47 ∶53 ∶0.2). The temperature of column was 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 280 nm. Result: Microwave extractive time was 15 min,the temperature was 120 ℃,the extraction solvent was 60%ethanol.The calibration curve was in good linearity with in the range from 0.059 to 0.59 μg(r=0.999 9). The average recovery was 99.36% ( n =6). Conclusion: The method is simple,rapid,as well as precise and reliable,and can be used for the determination of baicalin in S. baicalensis.关键词：microwave-assisted extraction(MAE);Scutellaria baicalensis;baicalin;determination
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发布时间：2024-01-04 - 摘要：Objective: To analyze the volatile oil from the leaves of Engelharditia roxburghiana . Method: The volatile oil was extracted from leaves of E. roxburghiana by steam distillation, the amount of the components from the volatile oil was separated and identified by GC-MS, and the relative content of each component was calculated by area normalization method. Result: Fifty five compounds were separated by GC and 33 of them were identified, which accounted for 95% of volatile oil. The major components were hexadecanoic acid (44.73%), phytol (14.71%), 9,12,15-octadecatriena (12.24%) et al. Conclusion: The analysis results can provide evidence for quality control and further exploitation of leaves of E. roxburghiana .关键词：Engelhardtia roxburghiana;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To set up a novel method for fast quality control of Paeoniae Alba’s extracting process based on near infrared spectroscopy (NIRS). Method: Aiming at the process of extracting, the content of paeoniflorin was determined by HPLC as the reference values, and the NIRS data were collected,and then partial least square (PLS) regression was applied to build the calibration model of paeoniflorin. Result: RMSECV of the model for paeoniflorin was 0.023 9, the correlation coefficient of the prediction samples between the predictive value and the real value was 99.86. The calibration model was used for testing samples, and the results were satisfied. Conclusion: NIRS can accurately determine the content of paeoniflorin, and indicates potential to be widely used in the extracting process of traditional Chinese medicine.关键词：NIRS;Paeoniae Alba;paeoniflorin;extracting process;PLS
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发布时间：2024-01-04 - 摘要：Objective: To study protein binding percentage of dehydroandrographolide in different plasma samples. Method: The protein binding rates of dehydroandrographolide in BSA plasma samples, human plasma samples and rat plasma samples were determined by ultrafiltration and HPLC. Result: The plasma protein binding rate of dehydroandrographolide with BSA, human plasma, rat plasma were (71.50±1.50)%, (79.91±2.51)%, (82.41±2.05)% respectively. Conclusion: The binding of dehydroandrographolide with plasma is middle degree.关键词：dehydroandrographolide;plasma protein binding percentage;Ultrafiltration
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发布时间：2024-01-04 - 摘要：Objective: To establish the method of determining zinc oxide content in Mayinglong Hemorrhoids Ointment by UV-VIS spectrophotometry. Method: The contents of zinc oxide in 3 batches of this preparation were determined by UV-VIS spectrophotometry. Chromogenic method of zinc reagent was used. Result: This method is higher sensitivity than titration. Conclusion: The UV-VIS spectrophotometry method is suitable for the determination of zinc oxide in this preparation.关键词：UV-VIS spectrophotometry;Mayinglong hemorrhoids ointment;zinc oxide
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发布时间：2024-01-04 - 摘要：Objective: To analyze the monosaccharides composition and their molar ratio in Tricholoma matsutake polysaccharide. Method: The monosaccharide units were hydrolyzed by trifluoroacetic acid from polysaccharide in T.matsutake and derivatized with hexamethyldisilane (HMDS) and trimethylchlorosilane (TMCS) to form trimethylsilyl derivative. The capillary chromatographic column HP-50 (0.32 mm×30 m) and FID detector were used in GC determination with inositol as internal standard. Result: Under the optimum conditions, the six standard monosaccharides were in baseline separation within 15 min, T. matsutake polysaccharide was composed of glucose, xylose and galactose with the molar ratio of 14.84 ∶1.42 ∶1.00. Conclusion: The results indicated that this method was effective and quick in analyzing the components of monosaccharides.关键词：Tricholoma matsutake;polysaccharide;gas chromatography
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发布时间：2024-01-04 - 摘要：Objective: To study stability and transformation of monotropein and deacetylaspcrulosidic acid, which were the main iridoid glucosides from Morindae Officinalis Radix. Method: The content of monotropein and deacetylaspcrulosidic acid from different years of Morindae Officinalis Radix were determined by HPLC to find out the stability in plants. The conversion of monotropein and deacetylaspcrulosidic acid in artificial gastric, water and artificial intestinal juice was studied to seek the stability in different pH solution. Result: The content ratio of monotropein and deacetylaspcrulosidic acid from different years of Morindae Officinalis Radix maintain similar. Monotropein converted to deacetylaspcrulosidic acid in artificial gastric. Conclution: The contents of monotropein and deacetylaspcrulosidic acid is relatively stable in Morindae Officinalis Radix. Monotropein can converted to deacetylaspcrulosidic acid in acidic condition.关键词：Morindae Officinalis Radix;monotropein;deacetylaspcrulosidic acid;HPLC;stability
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antioxidant effect of medicinal plants Vatica mangachapoi in Hainan province. Method: The sample extractions were prepared by accelerated solvent extraction (ASE) using solvent petroleum ether (MSO), ethyl acetate (EtOAc), ethanol (EtOH), methanol (MeOH); Combining with microplate assay model, the ASEs that had high antioxidant activity were screened quickly. Result: The antioxidant activity of different solvent ASEs from Vatica mangachapoi was evaluated by DPPH assay. The antioxidant activity was listed as follows: ethyl acetate>ethanol>petroleum ether>methanol; its IC50 values were 0.143 4, 0.501 8, 0.567 7, 0.693 7 g·L-1 respectively. The antioxidant of ethyl acetate ASE was better than ascorbic acid (IC50=0.203 4 g·L-1). Conclusion: The ethyl acetate ASE showed great DPPH scavenging activity, it’s hopeful to develop a new natural antioxidant.关键词：Vatica mangachapoi;accelerated solvent extraction;DPPH;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effective substance basis from 95% Alcohol elution constituents –bitter resolution and composition of Evodia rutaecarpa (Juss.) Benth. Var. bodinieri (Dode) Huang. Method:Isolation and purification were carried out by silica gel column chromatography, ODS RP- column chromatography, HPLC etc. Structural determination of the pure compounds was based on physico-chemical properties and various spectral data analysis. Result: Seven compounds were obtained from E. rutaecarpa. They were identified as narcissin(Ⅰ), Isorhamnetin-3-O-galactoside(Ⅱ), hyperoside (Ⅲ), isoquercitrin(Ⅳ), syringoside(Ⅴ),β-sitosterol(Ⅵ), dehydrolimonin(Ⅶ). Conclusion: Compound Ⅰ,Ⅳ,Ⅴ are isolated from E. rutaecarpa for the first time.关键词：Evodia rutaecarpa (Juss.) Benth. Var. bodinieri (Dode) Huang;substance basis;chemical constituents;character and taste;bitter
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determination of six major compounds in Dengzhanxixin injection, including chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid, scutellarin, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid. Method: The analysis was carried on a Zorbax SB-C18 (4.6 mm×150 mm, 5 μm) column. The mobile phase was composed of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 327 nm. The column temperature was at 30 ℃, The injection volume was 20 μL. Result: The linear ranges of six compounds were as follows: 0.079-5.065 mg·L-1 (r=0.999 9) for chlorogenic acid, 0.080-5.120 mg·L-1 (r=0.999 9) for caffeic acid, 0.074-4.750 mg·L-1 (r=0.999 9) for 1,3-O-dicaffeoylquinic, 0.391-25.050 mg·L-1 (r=0.999 9) for scutellarin, 0.038-2.423 mg·L-1 (r=0.999 9) for 3,5-dicaffeoylquinic acid, and 0.487-7.792 mg·L-1 (r=0.999 8) for 4,5-dicaffeoylquinic acid. The average recoveries were 96.72% (RSD 1.76%), 96.64% (RSD 1.69%), 101.81% (RSD 1.81%), 98.16% (RSD 2.22%), 99.02% (RSD 2.57%), 97.02% (RSD 1.52%) respectively. Conclusion: The method is convenient, rapid and accurate with good reproducibility and could be used for the quality control of Dengzhanxixin injection.关键词：Dengzhanxixin injection;chlorogenic acid;caffeic acid;1,3-O-dicaffeoylquinic acid;scutellarin;3,5-dicaffeoylquinic acid;4,5-dicaffeoylquinic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish the method of fingerprint analysis of the extract of galangal and identify its chemical constituents. Method: HPLC analysis was performed on an SunfireTMC18 (4.6 mm×200 mm, 5 μm) column with the acetonitrile-0.1% HCOOH gradient. Fingerprint was finished in 60 min. The flow rate was 1.0 mL·min-1.The monitoring wavelength was at 208 nm. The column temperature was at 30 ℃. The major components were identified by UPLC/Q-TOF MS. Result: Ten common peaks were separated on the fingerprint, 12 compounds were identified from the extract. Conclusion: The method is reliable, accurate and can be used as a quality control method for the extract of galangal.关键词：galangal;HPLC;fingerprint;UPLC/Q-TOF MS
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发布时间：2024-01-04 - 摘要：Objective: To study the difference in main chemical composition of Wujiwan decoction prepared with traditional method and processed granules. Method: HPLC was used with comparison aided by similarity evaluation. Result: There were 27 peaks in the chromatogram of the decoction prepared with traditional method and processed granules. The correlation coefficient of HPLC chromatograms between them was 0.992, 0.956 and 0.979. Conclusion: The main chemical substances and the composition ratio of the traditional decoction and compound granules of Wujiwan were similar but the content was different. This method support clinical application and quality control.关键词：Wujiwan;formula granules;characteristic chromatogram;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the content of rutin from the leaves of Ziziphus jujuba gathered in different areas and different periods for ascertaining the best harvest time and providing rational basis for the resource development. Method: The assay was performed on a Diamonsil C18 (4.6 mm×200 mm, 5 μm) column by an isocratic elution with methanol-0.1% acetic acid(34 ∶66) at a flow rate of 1.0 mL·min-1. The detection wavelength was at 257 nm.The column temperature was kept at 30 ℃. Result: Rutin was linear in the range of 0.221 6-2.216 μg (r=0.999 9). The average recovery ( n = 6) was 99.40% (RSD 2.42%). The content of rutin reached its maximum yields in May and June, however, the content drop down in July and August and rise again in September and October. Conclusion: This method was accurate, simple and stable. The content of rutin changing with time showed a U trend. It provided reference for quality evaluation criteria of the leaves of Z. jujuba关键词：leaves of Ziziphus jujuba;rutin;HPLC-UV
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发布时间：2024-01-04 - 摘要：Objective: Comparative study on the rosmarinic acid content from the roots, stems and leaves of wild and cultivated Sarcandra glabra . Method: HPLC was used to determine the content of rosmarinic acid, after the optimization of sample pre-processing and HPLC conditions. Result: The average content of rosmarinic acid in roots, stems and leaves from wild was 0.08%, 0.22% and 1.07% separately, and from cultivated was 0.09%, 0.24% and 0.96% separately. Conclusion: There was significant difference of rosmarinic acid content from roots, stems and leaves, while no significance was observed from wild and cultivated S. glabra.关键词：Sarcandra glabra;wild;cultivated;rosmarinic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of licorice juice as a processing excipient. Method: TLC identification, routine testing and determination by HPLC of ten batches of licorice juice were performed. Result: The identification method of licorice juice was established with both ammonium glycyrrhizate and liquiritin as reference substances for the first time. The content data of ash and soluble solid was obtained. The concentration data of glycyrrhizic acid and liquiritin was obtained. Conclusion: For the first time the research on quality standard of licorice juice was conducted systematically and the established standard can be used to control the quality of licorice juice as a processing excipient.关键词：licorice juice as a processing excipient;quality standard;TLC;glycyrrhizic acid;liquiritin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To determine metal elements in Galli Gigeriae Endotheliun Corneum by FAAS. Method: The samples were digested with mixed acid of nitric acid-perchlorate. Mg, Cu, Zn, Fe and Mn in Galli Gigeriae Endotheliun Corneum were determined by flame atomic absorption spectroscopy. Result: Galli Gigeriae Endotheliun Corneum was rich in Mg, Fe, Mn, Zn and Cu;recovery rate was 95.27%-104.47% and the relative standard deviation was 3.53% for each element. Conclusion: The detection method of metal elements of the Galli Gigeriae Endotheliun Corneum is established. The method is rapid, accurate and reliable.关键词：Galli Gigeriae Endotheliun Corneum;metal elements;flame atomic absorption spectrometry (FAAS)
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发布时间：2024-01-04 - 摘要：Objective: To analyze constituents in the volatile oils extracted from stems and leaves,root tubers and its processed products of Curcuma kwangsiensis. Method: Volatile oils of C. kwangsiensis were extracted through steam distillation,and the constituents were separated and identified by using GC-MS. Result: Thirty-seven chemical components were determined in volatile oil from stems and leaves of curcumae kwangsiensis,22 chemical components were determined in volatile oil from root tubers of C.kwangsiensis and 32 chemical components were determined in volatile oil from its processed products. Conclusion: Chemical components in volatile oils were different in three samples.关键词：Curcuma kwangsiensis;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: Explore the best compatibility proportion of rhizoma coptidis and Fructus Forsythiae’s anti-inflammatory effect. Method: Research the effect of each proportion of rhizoma coptidis and Fructus Forsythiae to the swelling of rat’s toe caused by injecting egg albumin. Give each groups berberine, forsythia, the ratio of the drug, aspirin, and saline.Respectively, 5, 30 min, 1, 2, 4, 6 h measure toe volume, calculate swelling rate. Establish an HPLC method for simultaneous determination of indicator ingredient of each proportion. Looking for links between them. Result: Berberine, forsythia proportion of the compatibility have an effect on inhibition of swelling. By analyzing the compatibility proportion of rhizoma coptidis and fructus forsythiae’s is 1 ∶1, it has the lowest swelling rate,therefore,has the most effective anti-inflammatory. There is the highest composite score when the compatibility proportion of rhizoma coptidis and fructus forsythiae’s is 1 ∶1. Conclusion: The best compatibility proportion of rhizoma coptidis and fructus forsythiae’s anti-inflammatory effect is 1 ∶1.关键词：rhizoma coptidis;Fructus forsythiae;medicine pair;compatibility proportion
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发布时间：2024-01-04 - 摘要：Objective: To estalish an accurate methed for the determination of Gastrodin in Tianma Zhuanggu Pills. Methed: Diamonsil C18(4.6 mm×250 mm, 5 μm)column in an oven at 25 ℃ was need, with a gradient elution which consist of acetonitrile and phosphoric acid solution and a UV detector at 220 nm, the flow rate was 1.0 mL·min-1. Result: The linear ranges of Gastrodin was 0.118 8-2.376 μg. The average recovery was 99.6%,RSD 2.44%. Conclusion: The method was simple and accurate, and can be used for the quality control of Tianma Zhuanggu Pills.关键词：Tianma Zhuanggu pills;gastrodin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of ethyl acetate fraction from Elaeagnus gonyanthes Caulis et Folium. Method: The medicinal plants were first extracted by 70% ethanol-water and then partitioned with petroleum ether, ethyl acetate and n-butanol, respectively. The chemical constituents of the ethyl acetate fraction were separated and purified by column chromatography methods and their structures were identified by physiochemical properties, thin-layer chromatography and spectra analyses. Result: 6 compounds were isolated and identified as β-sitosterol (1), lupeol (2), oleanolic acid (3), ursolic acid (4), α-amyrin (5), β-daucosterol (6), respectively. Conclusion: All the six compounds were isolated from this plant for the first time.关键词：Elaeagnus gonyanthes Caulis et Folium;Li nationality medicines;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the essential oils from stem of Uvria microcarpa Method: The essential oils were extracted by supercritical carbon dioxide extraction (SFE-CO2), and then the constituents were separated and identified by GC-MS. Result: Six-eight compounds were isolated and 37 compounds were identified, that composed about 85.73% of the total essential oils, the principal chemical constituents of the essential oils are benzoic acid, 8-oxo-9H-cycloisolongifolene, 3-(4-methoxyphenyl)-2-propenoic acid ethyl ester. Conclusion: There are some differences in the principal chemical constituents and content of the essential oil extracted by SFE and SD.关键词：Uvaria microcarpa;essential oils;supercritical carbon dioxide extraction;chemical constituents
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发布时间：2024-01-04 - 摘要：Objective: In order to make clear the components of the essential oil from the aerial part of Bupleurum chinense DC. f. vanheurckii (Muell.-Arg.) Shan et Y.Li and the antimicrobial activities. Method: Distillation in water vapor was applied to extract the essentialoil from B. chinense and meanwhile GC-MS was used to analyze the components of this plant. Its antimicrobial activities were investigated by plate diffuse and the minimum inhibitory concentration (MIC) was investigated by plate spread. Result: The essential oil was extracted successfully and 30 compounds were identified. Meanwhile the antimicrobial activities were tested and a broad-spectrum antimicrobial activity of the essential oil was found. Conclusion: Main components of the essential oil from B. chinense areterpenes and low molecular aliphatic hydrocarbon compounds. And the antimicrobial intensity to the tested strains is: Staphylococus aureus>Penicillium citrinum>Escherichia coil>Staphylococcus albus>Bacillus subtilis>Aspergillus niger>Aspergillus flavus.关键词：the aerial part of Bupleurum chinense DC. f. vanheurckii (Muell.-Arg.) Shan et Y.Li;the essential oil;GC-MS;antimicrobial activities
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发布时间：2024-01-04 - 摘要：Objective: To studying transdermal absorption patterns of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 from extraction of Panax notoginseng in vitro. Method: Using Franz diffusion cell for in vitro transdermal experiments, complete guinea pigs’ abdomen skin was used as transdermal barrier; HPLC was applied to determent the amount of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 which penetrated the test skin. Result: When no penetration enhancers like azone exsited, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 couldnot pass through test skin at all. When 2% azone was add in the Panax notoginseng extraction, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 could penetrate the test skin effectively. The sequence of its penetration capabilities was as follows: ginsenoside Rg1> notoginsenoside R1> ginsenoside Rb1. Conclusion: With the assisting of 2% Azone, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 can effectively penetrate complete guinea pigs’ skin.关键词：extraction of Panax notoginseng absorption;notoginsenoside R1;Ginsenoside Rg1;ginsenoside Rb1;Azone;transdermal absorption
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for the assay of bufalin (BL), resibufogenin (RBG) and study the pharmacokinetics of BL, RBG in rabbits plasma. Method: The sample blood from rabbits were injected venenum bufonis extract by 0.35 mg·kg-1 at 2,5,10,15,20,30,45,60,90 min. Protein precipitation with acetonitrile and liquid-liquid extraction was applied to purify plasma samples and used HPLC to determine the concentration of BL, RBG in it. The pharmacokinetics parameters were accessed by Kinetica software. Result: In this HPLC method, the separation, precision and accuracy of BL, RBG were well. The calibration curves were linear. The analytical recoveries were all more than 90%, intra and inter-day precision RSD were all less than 15.0% ( n =5). The main pharmacokinetics parameters were fitted by non-compartment models. Conclusion: The HPLC method can be used to determine the concentration and to investigate the pharmacokinetics of BL and RBG.关键词：venenum bufonis extract;bufadienolides;pharmacokinetics;rabbits
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发布时间：2024-01-04 - 摘要：Objective: To explore the effects of ultra-powder Liuwei Dihuang on recognition and basic fibroblast grouth factor(bFGF) in Alzheimers disease rats, and the mechanism of ultra-powder Liuwei Dihuang promoting recognition. Methold: The rat model of Alzheimer was established by Injection of Aβ1-40, rats were randomly divided into normal group, sham group, model group, Chinese medicines group, then at 5 d and 30 d rats were executed to evaluate the recognizing function, bFGF was assayed by ELISA and RT-PCR. Result: After modeling,rat escape latency was significantly longer, there was significant difference between the model group and Liuwei Dihuang group at 30 day (P<0.05); there was not significant differences between sham-operated group and Liuwei Dihuang group.In the normal and sham-operated group rat brain had a low level bFGF, it was increased in model group and the Chinese medicines group at 30 day, there was significant difference between the model group and Liuwei Dihuang group at 30 day point (P<0.05); there was not significant differences between sham-operated group and Liuwei Dihuang group. Conclusion: Rat recognition was declined after injection of Aβ1-40 intracerebral, ultra-power Liuweidihuang can promote recognitive function, can enhance bFGF expression in AD rat, which may be possible mechanism of yizhijiannao.关键词：cognitive function;Liuwei Dihuang Tang;Alzheimer disease;basic fibroblast growth factor
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发布时间：2024-01-04 -
Study of Yulian Cataplasm on Analgesic, Anti-inflammatory Actions and Irritation, Hypersensitiveness
摘要：Objective: Analgesic,anti-inflammatory actions and irritation, hypersensitiveness were observed to evaluate the pharmacodynamics and safety of Yulian cataplasm, and to provide the experimental basis for clinical applications of the cataplasm. Method: The experiment animals were randomly divided into Yulian cataplasm high, middle and low dose groups(12,6,3 g·kg-1).Carrageenan was used to induce rat paw edema. The mice inflammatory and algetic models were established by chemical irritation. Somatic writhing; foot swelling and capillary permeability were observed. The intact and damaged skins of rabbits were used to carry out irritation trial, and the hypersensitivity trial was conducted in guinea pigs. Result: Both middle and low dose groups of Yulian cataplasm could reduce rat paw swelling, middle dose group lasted longer duration of action. High and middle dose groups could decrease capillary permeability. All the three dose groups could reduce the writhing, and compared with excipient group (P<0.05, P<0.01). Irritation and hypersensitivity were not found. Conclusion: Yulian cataplasm could obviously exert both analgesic and anti-inflammatory actions, and no irritation and hypersensitivity were found.- 2
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Lichong decoction on p53 signaling pathway gene expression profile in human uterine leiomyoma cells in vitro. Method: The primary cultured uterine leiomyoma cells were treated with Lichong decoction. Using PCR p53 signaling pathway microarray, all RNA was gained and cleanuped from human uterine leiomyoma cells in blank control group and Lichong decoction group. RNA yield and quality were assessed by UV absorbance and denaturing agarose gel electrophoresis, first strand cDNA Synthesis, performing Real-Time PCR finally. Result: Lichong decoction group was compared with blank control group among 96 pieces genes in PCR p53 signaling pathway microarray, 17 in total(17.7%)genes expression had obviously changed, 10 pieces went down obviously(Fold Down-Regulation2). The genes were generally classified, according to their functions, into apoptosis, cell cycle, cell growth, proliferation and differentiation and DNA repair. Conclusion: Lichong decoction can regulate correlation genes of apoptosis,cell cycle,cell growth, proliferation and differentiation and DNA repair in p53 signaling pathway of uterine leiomyoma, whichshow that signal-transmit between in and out of cell is the key link in uterine leiomyoma process, Lichong decoction has the effect of regulation and control of p53 signaling pathway gene expression profile.关键词：uterine leiomyoma;p53 signaling pathway;gene expression profile;Lichong decoction
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Simo decoction on leptin and motilin(MTL) in serum of mice with chronic stress, and to explore the mechanism of Simo decoction on Functional dyspepsia(FD). Method: Fourty mice were randomized into normal group,model group,motilium group and Simo decoction group. The mice models were established by exerting the factors of irregular diet, reverse of day and night, binding and stimulating tails, then treated with distilled water, motilium and Simo decoction, respectively. The appetite and body weight of mice wereobserved. The contents of leptin and MTL were detected by enzyme-linked immunosorbent assay(ELISA). Result: The appetite and body weight of model group were lower than normal group(P<0.05),and the contents of leptin was increase and MTL deacreased in serum compared with the normal group(P<0.05).Simo decoction group and motilium group increased the appetite,body weight and MTL with decreased leptin compared with the model group (P<0.05). Simo decoction group had not statistically significant action compared with Motilium group. Conclusion: The therapeutic mechanism of Simo decoction for FD may probably lie in adjusting the secretion of leptin and MTL in serum.关键词：Simo decoction;chronic stress;appetite;body mass;leptin;motilin
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发布时间：2024-01-04 - 摘要：Objective: To investigate local therapeutic effects of Xiongzhi Tongyu San (XZTYS) in transdermal. Method: ① Animals were used and divided into 5 groups including model group, positive control group and treatment groups of large, middle and small dosage. XZTYS was given to rear legs of rats in transdermal daily for 3 days. Thereafter, rat paw edema was caused by inflammation action of carrageenan then extent of paw edema was measured. ② XZTYS were pasted to the back in transdermal daily for 7 days,and inflammatory model was induced by hypodermic injection of air and croton oil. Extravasation and granuloma within the cyst was measured. ③XZTYS was pasted on the ear of mice twice per day. Croton oil was used to establish inflammatory model. Weight of the ear was weighed after 4 hours. ④ XZTYS was pasted on the rear legs of rats daily for 3 days, threshold of pain was measured. ⑤ XZTYS was pasted on abdomen of the mice, the model of analgesia was established by the method of somatic wrenching, and threshold of pain was measured. ⑥ Sixty mice were used and divided into 6 groups including model group, two positive control groups and treatment groups of large, middle and small dosage. XZTYS was pasted on abdomen daily for 3 days. Dextran was iv used to cause model of micro circulation obstruction. The blood circulation of mesentery vessels was observed under dissection microscope. Result: XZTYS at the various doses could markedly suppress swelling of the rat paw and middle dose group extend threshold of pain. large dose group reduce swollen rate of the mice ear and enhance threshold of pain compared to model group. In addition, the middle or large dose group could improve obstruction of micro circulation of mesentery vessels. Conclusion: XZTYScan suppress reaction of acute inflammation and enhance threshold of pain in the rat and mouse models, and improve obstruction of micro circulation of mesentery vessels.关键词：Xiongzhi Tongyu San;animal model;inflammatory factors;swollen rate;threshold of pain;micro circulation
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发布时间：2024-01-04 - 摘要：Objective: To explore the possible mechanism of pharmacological activity of tilianin by observing the effects of Dracocephalum total flavoes on proliferation of rat vascular smooth muscle cells(VSMCs)induced by TNF-α. Method: The rat VSMCs were cultured in vitro, and the purity of cells was identified by SABC-Cy3 immunofluroescence technique. Cell proliferation model was established by stimulation with 0.02 mg·L-1 TNF-α. After TNF-α stimulation, cells were treated with three dose of Dracocephalum total flavoes(25,50,100 mg·L-1);MTT assay was used to evaluate the proliferation of cells. The expression of intracellular proliferating cell nuclear antigen(PCNA)were measured by immunoenzymatic histochemistry. Result: Compared with control group,three dose groups of Dracocephalum total flavoes could inhibit, in varying degrees, the proliferation and expression of intracellular PCNA of VSMCs induced by TNF-α, in a dose-dependent manner. Conclusion: The Dracocephalum total flavoes could inhibit the proliferation of VSMC induced by TNF-α, which might be one of pharmacological mechanisms of Dracocephalum total flavoes in treatment for atherosclerosis.关键词：Dracocephalum total flavoes;vascular smooth muscle cell;TNF-α;proliferation
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Soft Capsule Yindan Xinnaotong on regulation of lipid metabolism, antioxidation, vascular endothelial function in rat with hyperlipidemia. Method: Using hyperlipidemia animal model replicated by hyperlipidemia forage for 7 days, Yindan Xinnaoto capsule at doses of 2.25, 0.45 g·kg-1 ig for 1 month was treated. The serum TC, TG, HDL-C, LDL-C lever were detected, the levels of malondial-dehyde(MDA), superoxide dismalase(SOD), nitric oxide(NO), endothelin-1(ET-1) were assayed. Result: The levels of TG,TC, LDL-C, MDA and ET-1 in soft capsule of Yindan Xinnaotong treated groups were significantly reduced compared with model group. Conclusion: Soft capsule of Yindan Xinnaotong regulated the lipid metabolism, improved vascular endothelial function, increased the activity of scavenging free radicals in rat with hyperlipidemia.关键词：soft capsule of Yindan Xinnaotong;blood lipid;antioxidation;ET-1
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of action of Ganfukang capsule to human hepatocellular carcinoma cell line HepG2. Method: The effect of cell proliferation of Ganfukang capsule to human hepatocellular carcinoma cell line HepG2 was determined by MTT colorimetric. The cell cycle, cell apoptosis was analyzed by flow cytometry. Detect induction apoptosis protein Omi/HtrA2 and casepase-3 expression after the action of drug was observed by immunocytochemical method. Result: Ganfukang capsule had good inhibitory effect on HepG2 cell proliferation, showing a time-dose dependent. Application of Ganfukang capsule increased the proportion of HepG2 cells in G2 phase, reduced the proportion of G1 phase cells and S phase cells,and made the apoptosis rate increased. Conclusion: Ganfukang capsule can inhibit proliferation of hepatoma cell line HepG2, and promote their apoptosis.关键词：Ganfukang capsule;hepatoma carcinoma cell;flow cytometry;cell cycle;cell apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of total saponins of Panax japonicus(TSPJ) on neuronal apoptosis and expression of c-fos, c-jun proteins in rat cerebral injury induced by ischemia and reperfusion. Method: Sixty SD rats were randomly divided into 6 groups:control group, model group, nimodipine group, TSPJ 200,100,50 mg·kg-1groups.The models of cerebral ischemia-reperfusion injury was reproduced by middle cerebral artery occlusion(MCAO). HE staining was used to assay the pathological changes;TUNEL stainning was used to detect apoptotic neurons;immuno-histochemistry was used to observe the protein expression of c-fos and c-jun. Result: Compared with the control group, the neuronal apoptosis was increased and the expression of c-fos、c-junhad remarkably enhanced after cerebral ischemia reperfusion(P<0.01);TSPJ improved the pathological changes,TSPJ(200,100 mg·kg-1)group significantly inhibited the expression of postitve neuronal cells of TUNEL and the expression of c-fos, c-jun decreased (P<0.01,P<0.05). Conclusion: TSPJ can decrease the number of apoptotic cells after cerebral ischemia and reperfusion by down-regulating the expression of c-fos and c-jun,which may be one of mechanisms of neuroprotective effects.关键词：total saponins of Panax japonicus;cerebral ischemia-reperfusion injury;apoptosis;C-FOS;c-jun
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发布时间：2024-01-04 - 摘要：Objective: According to the traditional Chinese medicine (TCM) theory that lung injury was resulted from chilled body shape and cold drink and"Yang "Deficiency was caused by over-strain and internal injury", we combined two or three of four Lung-Yang Deficiency factors to compare the effects of these different combinations in. mice Lung-Yang Deficiency models. The four factors were termed as smoking in the special box, swimming in ice water, drinking ice water, and taking the methimazole ice solution. Method: Seventy ICR mice were randomly divided into four model groups and normal group. Each group with 14 animals was treated for 10 days. We observed the general physical signs (grip strength and self-activity), measured MDA and SOD activity of lung tissue and serum, and calculated the organ indexes. Result: The body weight growth decreased, the self-activity and grip strength reduction, and the heart and lung indexes increase were observed in all model groups. In addition, the effects of three-factor combination groups on physical signs as well as MDA and SOD of lung tissue and serum were better than which of the two-factor combination groups, resulting in that swimming in ice water was more effective than drinking ice water. These results showed that the combination of smoking, swimming in ice water, and drinking ice water was the best method to induce Lung-Yang Deficiency. Conclusion: Different combinations of four Lung-Yang Deficiency factors indicated different effects. Among these combinations, the three-factor combination (smoking + swimming in ice water + drinking ice water) was cinsistent with TCM theory about Lung-Yang Deficiency, which may be induced by several factors such as chilled body shape, cold drinks and over-strain.关键词：Lung-Yang Deficiency;model;multi-factors
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of total alkaloids in Leonuri Herba(LH) on mice benign prostate hyperplasia (BPH). Method: Benign prostatic hyperplasia model was established by subcutaneous injecting of testosterone propionate 5 mg·kg-1·d-1 consecutively for 21 d. The mice were randomly divided into 6 groups:160, 80, 40 mg·kg-1 alkaloids dosage group, Longbishu capsule suspension group,model group. normal control group. Every group was daily given the corresponding drugs for 30 days. The prostate index and histomorphology changes of prostate gland, testicle, epididymis, thymus gland and spleen that were determined. Result: Compared with model group, the total alkaloids in LH at the dosage of 160,80,40 mg·kg-1 could significantly reduce the prostate index of BPH. The prostate index of different groups was (0.53±0.1). (0.51±0.1), (0.64±0.18) mg·g-1 respectively.The total alkaloids in LH could significantly improve the pathological changes of testicle and epididymis concomitant with BPH. The total alkaloids in LH at the dosage of 160,80,40 mg·kg-1could significantly improve the atrophy of thymus gland and spleen. The thickness of thymus cortical and the number of lymphocyte was (24.16±2.2),(26.28±3.20), (26.32±3.31) μm and (60.3±4.5), (68.2±5.2), (53.3±4.2). The spleniclymphnodules and the number of lymphocyte was respectively(14.18±2.3),(16.14±3.0), (29.26±3.52) μm and (17.2±2.0), (15.3±3.1), (28.3±3.4). Conclusion: Total alkaloids in LH has good therapeutic actions on mice BPH.关键词：total alkaloids in LH;testosterone propionate;benign prostatic hyperplasia;mice model
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发布时间：2024-01-04 - 摘要：Objective: To Explore the relationship between pathogenesis of Tourette syndrome(TS) and dominant with presynaptic dopamine nerve fibers. At the same time to observe the effect of Jing’an oral liquid on distribution of dopamine transporter of TS model rats. Method: The model of TS was established by peritoneal injection of 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) 1 mg·g-1 for 21 days. SD rats were randomly divided into 4 groups, the control group, the model group,Jing’an oral liquid group(11 g·kg-1) and tiapride group(0.025 g·kg-1), and drugs were orally administered for 21 days. In each group with 99 Tc-TRODAT-1 as a ligand, the distribution of dopamine transporter in rat brain was measured by γ-radiation immunity Counter. Result: In model group, three sites’ (striatum, cerebral cortex and hippocampus) radioactive uptake ratio (CMP / GB) was significantly higher than that in control group (P<0.05).In Jing’an oral liquid group three sites’ radioactive uptake ratio (CMP/GB ) was lower than that in model group (P<0.05) with a statistically significant difference. Conclusion: Compared with normal group, the distribution of dopamine transporter(DAT) in model group brain significantly is increased, which may cause the dopamine hyperfunction and lead to TS,.Jing’an oral liquid may be play a therapeutic role by reducing the distribution of DAT, thereby reducing the presynaptic dopamine neurons over-dominance.关键词：Jing’an oral liquid;Tourette syndrome(TS);dopamine transporter (DAT);rat
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of aqueous extract from Zingiberis Rhizoma Recens on excitatory amino acid(EAA) in brain tissue after cerebral ischemia reperfusion(CIR) in rats. Method: Male SD rats were randomly assigned to 6 groups as following:sham-operation group, model control group, nimodipine group and high, middle and low dosage groups of aqueous extract from Zingiberis Rhizoma Recens. CIR model in rats was reproduced by Pulsinelli’s "Four Vessel Occlusion" method. The contents of glumatate(Glu), asparate(Asp), and glycine(Gly) in brain tissue were analyzed by high performance liquid chromatography with fluorescent detection. Atomic spectrophotometry was used to measure Ca2+ content in brain tissue. The changes of brain water content were measured by the wet and dry weight methods. Spectrophotometric assay was used to measure the activity of superoxide dismutase ( SOD) and the contents of malondialdehyde(MDA) in brain tissue. Result: The contents of Glu, Ca2+, water, and MDA in brain tissue were increased,the activitiyof SOD was declined significantly in CIR model group,compared with the sham operation group. Aqueous extract from Zingiberis Rhizoma Recens could decrease the contents of Glu, Ca2+ and water in brain tissue, increase the activities of SOD, but there was no significant differencein the content of Gly, compared with model control group. Conclusion: The action of aqueous extract from Zingiberis Rhizoma Recens on CIR may be involve in declinning EAA neurotoxicity, decreasing intracellular Ca2+ overload and increasing antioxidant activity.关键词：Zingiberis Rhizoma Recens;cerebral ischemia reperfusion;excitatory amino acid
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发布时间：2024-01-04 - 摘要：Objective: To study the antibacterial activity of Thladianthea dubia fruits in vivo and in vitro, and screen the extracts of antibacterial activity. Method: By methods of tube continuous dilution and agar plate diffusions to 14 strains of bacteria, for example Staphylococcus aureus , minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of different extracts of T. dubia fruits were determined in vitro . We chosen mice infected by the type strain of S. aureus 26002-6 as models to test the protection of the extracts of T. dubia fruits. Result: With regard to volatile oil of T. dubia fruits (VTDF), the MICs to the type strains of S. aureus 26001-26, Staphylococcus aureus26002-6, Escherichia colt44102-3a3, S.albus 26069-5, Salmonella paratyphi A 50001-25, S.paratyphi B 50076-1, S.typhosa50071-10 were respectively 0.17, 0.08, 0.33, 0.17, 0.33, 0.33, 0.66 g·L-1, the MBCs were 0.17, 0.17, 0.33, 0.33, 0.66, 0.66, 1.32 g·L-1;and the MICs to the clinical-separated strains of S. aureus, Escherichia colt0415, E.colt0416, Enterococcus faecalis0318, Proteus mirabilis, Acinetobacter baumannii0207, S. pneumoniae 09030813 were respectively 0.66, 0.66, 0.66, 0.66, 0.08, 0.66, 1.32 g·L-1, the MBCs were 1.32, 1.32, 1.32, 1.32, 0.08, 1.32, 2.64 g·L-1. However, water extract and polysaccharides of T. dubia fruits (WTDF, PTDF) had antibacterial activity on partial bacteria in vitro . VTDF and PTDF might have obvious protective effect on infected mice induced by S. aureu , WTDF might have weak protective effect on infected mice induced by S. aureu . Conclusion: The antibacterial activity parts of T. dubia fruits are VTDF and PTDF.关键词：Thladiantha dubia fruit;antibacterial activity;volatile oil;polysaccharide
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect and its mechanism of Kanli granule on heart failure hemodynamics in chronic heart failure by partial coarctation of abdominal aorta in rats. Method: The chronic heart failure (CHF) model was induced by partial coarctation of abdominal aorta in rats. The CHF rats were feeded with Kanli granule(unprepaired herbal medicine 12 g·kg-1·d-1)for 24 weeks,and the captopril group(3.375 medicine given Kanli granule 12 g·kg-1·d-1)for 24 weeks. After 24 weeks, cardiac hemodynamics parameters, left ventricular mass index (LVMI), Cardiac mass index (CMI),left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), left ventricular end-diastolic pressure(LVEDP), collagen volume fraction(CVF), collagenⅠ/Ⅲ ratio were measured pathologically. Myocardial cell and interstitial collagen were observed by HE and VG stain. Result: Kanli granule availably improved cardiac hemodynamics parameters including LVSP,LVEDP, ±LVdp/dtmax,decreased CVF and CMI (P<0.05) including LVMI(P<0.01), meanwhile increased Ratio of type I/1I)(P<0.05). Conclusion: Kanli granule can availably improved cardiac hemodynamics parameters of rats with CHF, regulate over-degradation of myocardial collagen and prevent ventricle expanding which are related to relieving the myocardial extracellular matrix remodeling of CHF.关键词：Kanli granule;chronic heart failure;cardiac hemodynamics
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发布时间：2024-01-04 - 摘要：Objective: To discuss mechanism and effect of Compound Geshanxiao granules on gastrointestinal motility. Method: Mouse intestinal propulsive activity was inhibited by atropine to observe the movement of small intestine. The rat functional dyspepsia model was established by clipping tail stimulation. The contents of nitrogen monoxidum(NO) in gastric mucous membrane, the level of rat blood gastrin(GAS) and motilin(MTL) were measured. Result: Compound Geshanxiao granules could apparently increase intestinal propulsive activity in mice, and decrease the contents of NO in gastric mucous membrane, meanwhile increase the contents of GAS in serum and MTL in plasma of functional dyspepsia model in rats. Conclusion: Compound Geshanxiao granules can improve intestinal propulsive activity, the mechanism may be related to decreasing the contents of NO in gastric mucous membrane and elevating the level of rat GAS in serum and MTL in plasma.关键词：Compound Geshanxiao granules;gastrointestinal motility;nitrogen monoxidum;gastrin;motilin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Fuke Qianjin tablets on the blood cells and histopathological manifestationsof acute pelvic inflammatory in rats. Method: The model of acute pelvic inflammation was established by injecting three kinds of bacteria (Escherichia coli,Mycoplasma urealyticum and Staphyloccocus aureus Rosenbach) into the uterus. The rats were randomLy divided into 8 groups and the patterns of blood cells were tested after 21 days of treatment. Result: Compared with normal group and shaml group, the white blood cells and proportions of neutrophils in model group were significantly lowered but proportions of lymphocytes increased which indicated a significant pelvic inflammation. Compared with model group, the white blood cells and proportions of neutrophils in treat groups were significantly increased but proportions of lymphocytes lowered. Conclusion: Fuke Qianjin tablets can influence the bloods cells and immunocytes to protect pelvic tissues.关键词：Fuke Qianjin tablets;acute pelvic inflammation;blood cells
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发布时间：2024-01-04 - 摘要：Objective: To compare the effects of the same compound (Yiqi Yangxue decoction and Buzhong Yiqi decoction) prescription containing Astragali Radix (AR) and Hedysari Radix (HR),respectively, on immunologic functions of mice. Method: The sixty mice were divided randomly into six groups:the control group, the model group, Yiqi Yangxue decoction containing AR group, Yiqi Yangxue decoction containing HR group, Buzhong Yiqi decoction containing AR group and Buzhong Yiqi decoction containing HR group. The mice were injected peritoneally with cyclophosphamide to establish immunosuppressive model,and spleen lymphocyte Proliferation, IL-1,IL-4 and serum hemolysin level, NK cells activity were observed Result: Afteradministration, the immunologic functions were significantly increased. The compounds congtaining HR were more significant than compounds containingg AR in spleen lymphocyte proliferation, but in other respects, the two compounds whether congtaining AR or HR had no obvious diffference. Conclusion: Both Yiqi Yangxue decoction and Buzhong Yiqi decoction containg HR are better thancompouds congtaining AR in improving cellular immune function.关键词：Yiqi Yangxue decoction;Buzhong Yiqi Decoction;Astragali Radix;Hedysari Radix;immunologic function
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Dracocephalum moldavica petroleum ether extract and ethyl acetate extract on hyperlipidemia. Method: The rat hyperlipidemia model was established by administration of high rat emulsion. The following indexes were determined: total cholesterol(TC), triglyceride (TG), low density ipoprotein-cholesterol(LDL-C), high density lipoprotein-cholesterol (HDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), alanine aminotransferase (ALT), aspartate aminotransferase (AST), the malondialdehyde (MDA) and superoxide dismutase (SOD) of hepatic tissue after administration of 10 weeks therapy Light microscope was used to observe the steatosis of hepatic tissue in hyperlipidemia rat. Result: Compared with model group, D. moldavica petroleum ether extract could decrease the serum level of TG, LDL-C, ALT, AST, MDA (P<0.01 or P<0.05), increase the serum level of HDL-C, SOD (P<0.01), increase the liver homogenate level of SOD (P<0.05),and lower the liver homogenate level of MDA (P<0.01);Compared with model group, D. moldavica ethyl acetate extract could lower the serum level of TC, LDL-C, ALT,AST, MDA (P<0.01), increase the serum level of SOD (P< 0.05), and increase the liver homogenate level of SOD(P<0.01), lower the liver homogenate level of MDA (P<0.01). Conclusion: D. moldavica extract can regulate lipid metabolism disorders and reduce the incidence of fatty liver and its modulation may be related to its antioxidant capacity.关键词：Dracocephalum moldavica of petroleum ether extract;D. moldavica of ethyl acetate extract;hyperlipidemia;rat;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Longdan Xiegan pill (LD) on expression of multidrug resistance protein and neutrophil CD18 and tclarify the possible mechanism of LD to clear dampness-heat of liver and gallbladder. Method: Twenty healthy Wistar rats were randomly divided into 4 groups:control group,ANIT-treated model group,LD 5.0 g·kg-1group and LD 2.5 g·kg-1group. LD groups were administered orally for 8 days,while control group and model group were administered orally with equal distilled water,once daily. After the last administration for 1 h,model group and LD groups were treated with 100 mg·kg-1 ANIT,while control group was administered with equal peanut oil. After administration of ANIT 72 hours,The liver total RNA of all rats wwas extracted and the levels of MDR1a, MDR1b, MDR2, MRP1 and MRP2 mRNA in liver tissue were detected by reverse transcription polymerase chain reaction (RT-PCR). The expression of CD18 was measured by using flow cytometer. Total leukocytes and granular leukocytes were counted by using microcomputer controlled hematology analyzer. Result: The expression of MDR1a, MDR1b mRNA in model group wwas significantly higher than that of control group (P<0.05,P<0.01). The expression of MDR2, MRP1, MRP2 mRNA was not obviously changed. LD 5.0,2.5 g·kg-1 group had no obvious effects on expression of multidrug resistance protein in cholestatic rats. LD groups could reduce total leukocytes granular leukocytes and CD18 fluorescence intensity. Conclusion: LD has protection in ANIT-induced cholestatic rats,which is similar to the syndrome of dampness-heat of liver and gallbladder. The protection mechanism maybe mainly related to inhibiting endothelial-leukocyte adhesion,inhibiting inflammatory responses in bile ducts, alleviating biliary injuries,increasing bile flow of intrahepatic bile ducts,not related to the expresion of MRP1 and MRP2 mRNA in biliary epithelial cells.关键词：Longdan Xiegan Pill;cholestasis;multidrug resistance protein;multidrug resistance-associated protein;CD18
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发布时间：2024-01-04 - 摘要：Objective: The mechanism of Jiawei Sini San(JWSNS) in treating hepatic fibrosis was rexplored and studied by observing the expression of collagen Ⅳ and transforming growth factor-beta (TGFβ1)mRNA in liver. Method: Fifty animals were randomly divided into 4 groups, in addition to 10 in the control group, the remaining 40 were treated with dimethylnitrosamine (DMN, diluted with saline to 5 g·L-1) to 10 mL·kg-1 ip, once daily for 3 d of each week, which lasted 4 weeks.The groups of treatment were fed with JWSNS gavage. Collagen Ⅳ and TGF-β1 were detected in liver samples by RT-PCR. Result: CoL-Ⅳand mRNA of Liver tissue in JWSNS group was 5.136 ± 1.32, TGFβ1 mRNA was 4.839±0.48, Compared with the pathological model group (CoL-Ⅳ mRNA 10.369±1.69, TGF β1 mRNA 6.307±0.85) the result were significantly different (P<0.05). Conclusion: The mechanism of treating hepatic fibrosis with JWSNS appears to be due to the enhancement of degradiation of Collagen Ⅳ and TGF-β1.JWSNS can treat hepatic fibrosis effectively.关键词：Jiawei Sini San;hepatic fibrosis;Collagen Ⅳ;transforming growth factor-beta 1
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发布时间：2024-01-04 - 摘要：Objective: To observe the alleviating effects of Jingfang Baidu San on acute alcoholism in mouse. Method: The mice were randomly divided into five groups, including normal,group, model group, Jingfang Baidu San high,moderate,low-dose group( the dose was calculated by the amount of crude drug 32.4,16.2,8.1 g·kg-1,and ig given before or after 30 minutes of drinking alcohol).Except normal group, the model of acute alcoholism was established by drinking 14 mL·kg-1 56° Hongxing Erguotou alcohol once. The rate and time of alcohol drink were observed after preventing treatment, and the death rate and sober-up time were observed after treatmen. Alcohol density in serum and the activity of 14 mL·kg-1(ADH) in liver and stomach tissue were observed. Result: Compared with model group,the rate of alcohol drink in Jingfang Baidu San group was decreased (P<0.05),and the time of alcohol drink was longer(P<0.01). The death rate in Jingfang Baidu San group was decreased(P<0.05 orP<0.01), and sober-up time was shorter (P<0.05 orP<0.01) after treatment.Compared with normal group.The alcohol density in serum was increased and the activity of ADH in liver and stomach tissuedecreased in model group (P<0.01). Compared with model group,the alcohol density in serum of each group were decreased(P<0.01),the activity of ADH in liver and stomach tissue in Jingfang Baidu San group were increased(P<0.05 orP<0.01).Conclusion Jingfang Baidu San can improve the activity of ADH in liver and stomach tissue in acute mouse alcoholism,and promoting alcohol metabolism to reduce alcohol density, so Jingfang Baidu San can alleviate acute alcoholism in mise effectively.关键词：acute alcoholism;Jingfang Baidu San;ethanol dehydrogenase;death rate;sober-up time
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Shengbao granula on immunological function in mice with immunological deficiency. Method: Carbon clearance test, T lymphocyte esterase conversion rate test, 2,4-dinitrofluorobenzene-induced delayed type hypersensitivity test(DTH),and hemolysin antibody formation induced by chick erythrocyte were employed to evaluate the effectsa of shengbao granula on immunological function. Result: Shengbao granula(7.50, 3.75 g·kg-1)group could increase the K value and the weight index of spleen in d14 after administration (7.50, 3.75 g·kg-1)group increased peripheral T lymphocyte proliferation in d7, enhanced murine ear swelling in d13, and could not increased hemolysin antibody in d10. Conclusion: Shengbao granula significantly improves nonspecific and cellular immunity function in mice with immunological deficiency.
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of combined Oldenlandia diffusa and Scutellariae Barbatae Herba superfine powder on tumor growth of transplanted hepatocarcinoma in mice and expressions of Bcl-2 and Bax proteins in tumor tissues. Method: Sixty healthy Kunming mice,transplanted by H22 hepatocarcinoma cells,were divided into 6 groups,including model group, Ruanjian oral liquid group, combined O. diffusa and Scutellariae Barbatae Herba common grinding powder group and three groups of combined O. diffusa and Scutellariae Barbatae Herba superfine powder in high dose,medium dose and low dose.The mice were executed after ten days of treatment and tumor tissues were excised.The size of tumor tissues were measured and tumor inhibition rate was calculated.And expression of Bcl-2 and Bax was detected by using immunohistochemical method. Result: The tumor inhibition rate of Combined O. diffusa and Scutellariae Barbatae Herba superfine powder group in high dose was 47.55%,which was nearly same as Ruanjian Oral Liquid group.Compared with the model group, expression of Bcl-2 protein was decreased in the groups of Combined O. diffusa and Scutellariae Barbatae Herba superfine powder in high,medium and low dose; expression of Bax protein was increased in the groups of combined O.diffusa and Scutellariae Barbatae Herba superfine powder in high and medium dose. Compared with common grinding powder groupvexpression of Bcl-2 protein was increased and the expression of Bax Protein was decreased in the high group of Combined O.diffusa and Scutellariae Barbatae Herba superfine powder. Conclusion: Combined O. diffusa and Scutellariae Barbatae Herba superfine powder can inhibit the growth of tumor of H22,reduce the expression of Bcl-2 protein and increase the expression of Bax, which is worth further studying in antitumor action.关键词：combined O. diffusa and Scutellariae Barbatae Herba;superfine powder;H22 hepatocarcinoma cell;Bcl-2;Bax
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发布时间：2024-01-04 - 摘要：Objective: To study the killer activity of cytokine-induced killer (CIK) cells treated with Chinese medicine polysaccharides against the ovarian cancer cell line SKOV3 under different conditions, and explore the possibility of adoptive immunotherapy combined with Chinese medicine in the treatment of ovarian cancer. Method: The experiment were divided into four groups, such as CIK group, PBDC-CIK group, SKOV3Ag-PBDC-CIKgroup and SKOV3Ag-ADC-CIKgroup. CIK cells derived from the peripheral blood (PB) were induced by cytokine and grifola polysaccharide (100 mg·L-1), pachyman (100 mg·L-1) and astragalan (100 mg·L-1).while dendritic cells (DC) derived from PB and ascites by SKOV3 tumor cell lysate. The cytotoxic activities of CIK group, PBDC-CIK group, SKOV3Ag-PBDC-CIK group and SKOV3 Ag-ADC-CIK group against SKOV3 were analyzed, as well as the proliferation of CIK cells between groups. Result: ①The proliferation rate of CIK cells co-cultured with DC significantly increased.(P<0.01) ②CIK cells induced by Chinese medicine polysaccharides were cytotoxic to SKOV3 cells, which increased with effector: target (E ∶T) ratios,which were(9.12±0.21)%、(10.15±0.27)%、(11.20±0.34)% and (12.73±0.43)% (P<0.05), ③ The cytotoxic activities of SKOV3Ag-PBDC-CIK group against SKOV3 cells were significantly higher than that of both DC-CIK group and CIK groups (P<0.05, P<0.01). ④ There was no significant difference in killing activity between SKOV3Ag-PBDC-CIK group and SKOV3Ag-ADC-CIK group. Conclusion: DC pulsed with tumor lysate antigens of SKOV3 could enhance the proliferation and cytotoxicity of CIK cells induced by Chinese medicine polysaccharides. And DC derived from ascites could serve as an important source in adoptive immunotherapy.关键词：ovarian neoplasms;CIK cell;Dendritic cells;Chinese medicine polysaccharides;cytotoxicity
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发布时间：2024-01-04 -
Intervention Effects of Tangpingjian on Inflammatory Cytokines in Rats with Diabetes Mellitus Type 2
摘要：Objective: To investigate the effects of Tangpingjian(TPJ) on insulin resistance(IR) and serum Adiponectin (Adip),leptin,tumor necrosis factor alpha(TNF-α) in type 2 diabetic(T2DM) rats. Method: The type 2 diabetic rats model was established by the injection of small-dose streptozotocin (STZ 30 mg·kg-1) combined with high-energy feeding. The rats were randomly assigned to the model group,TPJ high-dose, TPJ small dose group and normal control groups. Rats in TPJ high-dose group were performed intragastric administration of 14.4 g·kg-1·d-1 crude drug of Tangpingjian,and rats in TPJ small dose group were administrated with 7.2 g·kg-1·d-1 crude drug of Tangpingjian,the other two groups were administrated with distilled water by equal volume. All four groups had been treated for four weeks. Fasting blood-glucose (FBG),Fasting insulin(FINS),TNF-α, Adip and Leptin of rats were measured after four weeps. Calculation of insulin sensitivity index was carried out(IAI). Result: TPJ could decrease FBG,FINS,leptin and TNF-α remarkably,and improve insulin sensitivity index and Adip activity obviously in experimental rats. It has remarkable difference compared with model group (P<0.01,P<0.05). Conclusion: TPJ can improve T2DM rat inflammatory response, reduce hyperinsulinemia, increase the insulin sensitivity, and improve insulin resistance.关键词：insulin resistance;adiponectin;leptin;tumor neerosis faetor-α;tangpingjian- 3
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of nitidine chloride(NC) and hydroxycamptothecine(HCPT) on the development of zebrafish embryo. Method: The 6 h and 48 h post-fertilization zebrafish embryos were randomly treated with NC (5.00,3.15,2.00,1.58,1.12 mg·L-1) and HCPT ( 16.00,10.00,6.50,4.00,2.50 mg·L-1). The death rate and malformation rate of zebrafish embryos were observed after 72 h. Result: 50% lethal concentration (LC50)of was 1.66 mg·L-1 and 50% malformation effect concentration (EC50) was 1.33 mg·L-1 in NC 6 hpf group. LC50 was 2.51 mg·L-1 and EC50 was 2.02 mg·L-1 in NC 48 hpf group. In HCPT 6 hpf group,LC50 was 4.21 mg·L-1,EC50 was 3.29 mg·L-1;LC50 was 7.70 mg·L-1,EC50 was 6.18 mg·L-1 in the HCPT 48 hpf group. Death rate and malformation rate were decreased when the drugs concentration reduced and test time were delayed. Conclusion: zebrafish embryos of 6 hpf are more sensitive to NC and HCPT than embryos of 48 hpf; both NC and HCPT can cause embryotoxicity to zebrafish; NC is more toxic to the development of zebrafish embryos than HCPT.关键词：nitidine chloride;hydroxycamptothecine;zebrafish;embryotoxicity
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinic influence and corresponding immunological function changes in treating malignant tumor patients by Yiyuan Huoxue Tang. Method: Fifty-six malignant tumor patients in chemotherapy were randomly divided into two groups. Twenty-nine patients in treatment group were treated with Yiyuanhuoxue recipe, twenty-seven patients in control group were treated with chemotherapeutical agents. The expression of T lymphocyte subsets, immunoglobulins,Ag-nors observed before and after treatment in both groups. Result: Yiyuan Huoxue Tang had defend function for cellular immune of malignant tumor patients in chemotherapy,could elevate the level of CD3,CD4,CD4/CD8 and Ag-Nors. There was no significant influence in immunoglobulin. Conclusion: Yiyuan Huoxue Tang has good therapeutic effects on the cellular immune function.关键词：malignant tumor;Tlymphocyte subsets;immunoglobulins;Ag-Nors
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发布时间：2024-01-04 - 摘要：Objective: To observe treatment effects of Jiawei Sancai Fengsuitang on eukopenia after chemotherapy. Method: Eighty-nine patients ientered treatment group. Treatment group was treated with oral Jiawei Sancai Fengsuitang, daily; The control group was treated with oral 20 mg of leucogen and batyl alcohol 100 mg three times daily, times. Result: Treatment group increased the clinical curative effect, white leukocyte count and physical state after treatment were superior to control. So it showed statistically significant (P<0.01). Intercurrent infection and chemotherapy were lower than those of the control group, and it has statistical differences (P<0.05). Adverse reactions were not found in the treatment group. Conclusion: Jiawei Sancai Fengsuitang can improve the physical state and immunological functions, and can reduce infection and chemotherapy delay rate.关键词：Jiawei Sancai Fengsuitang;leukopenia;supplementing QI and nourishing YIN for enriching blood
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发布时间：2024-01-04 - 摘要：Objective: In order to promote rational drug use in the clinic, to analyze the characteristics, general patterns and influencing factors of adverse drug reaction(ADR) induced by traditional Chinese medicine preparations. Method: A retrospective analysis method was adopted. Seventy ADR case reports which were collected form 2006 to 2008 in our hospital were analyzed statistically in respect of patients’ age, drug categories, routes of administration, organs and systems involved and clinical manifestations etc. Result: 91.63% of the ADR were induced by traditional Chinese medicine injections. The injections in therapying cardiovascular and cerebrovascular diseases took the most (account for 60%). Conclution:Timely reporting of traditional Chinese medicine preparation-induced ADRs, awareness on ADR monitoring should be strengthened to ensure safe and effective in the clinic.关键词：adverse drug reaction;Traditional Chinese Medicine preparation;cardiovascular and cerebrovascular drugs;rational drug use
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发布时间：2024-01-04 - 摘要：Objective: To study the dosage of clinical prescription in surgery of traditional Chinese medicine(TCM) based on the data from two affiliated hospital of Guiyang college of TCM, and clear the situation and characteristics of the dosage of Chinese cut crude drug in Guiyang city. Method: The methods of stratified sampling and random sampling were used to investigate 5 327 broth prescriptions of TCM for surgery. We constructed the database, then applied Weka KDD software to analyze. Result: We identified the frequency and clinical dosage intervals of 300 drugs, and the practical dosage showed some difference from that in the pharmacopoeia. Most of drug dosage centralized the three intervals: (9-12], (12-15],(18-21],and the concrete dosage were 10, 12, 15, 20 g, etc. Conclusion: The situation and characteristics of the dosages of clinical prescription in Guiyang city are coincided with the national level of TCM for surgery.关键词：traditional Chinese medicine for surgery;Chinese cut crude drug;dosage;pharmacopoeia of the people’s republic of China
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发布时间：2024-01-04 - 摘要：Objective: To choose a treatment for cough variant asthma with effective drugs, to compare ketotifen with montelukast joint MeipuQing, BDP spray. Method: The inclusion criteria found 120 cases of patients. They were randomly divided into two groups(60 cases each). Two groups were treated with meipuqing kechuanning ig,and Beclomethasone Dipropionanate Aerosol as basic treatment program. Control group was also given ketotifen(1 mg, 2 times a day) orally.Observation group was also given montelukast (5 mg, qn) orally. Two groups were treated for 15 weeks as a course, then to evaluate the clinical efficacy. Result: The clinical control rate was 63.33%, 95.00% total efficiency in observation group. Clinical control rate of the control group 46.67%, 75.00% total efficiency in control group. The difference in two groups was significantly(P<0.05). Daytime symptom scores, nighttime symptom score in the two groups were less than those before treatment 2(P<0.05); but the observation group decreased more significant than the control group,group(P<0.05). Forced expiratory volume in one second(FEV1) and FEV1% for 2 groups the difference before and after treatment were significant (P<0.05). After treatment, 2 groups showed significant difference(P<0.05). The incidence of side effects in observation group was 3.33%; control group, 10.00%. There was significant difference was P<0.05. Conclusion: Montelukast and ketotifen are effective for cough variant asthma treatment, the therapeutic effect of montelukast is even better.
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发布时间：2024-01-04 - 摘要：Literatures about the effect of traditional Chinese medicine on vascular smooth muscle cells(VSMC) have been analyzed. Atherosclerosis(AS) has big damage on humans. Phenotypic, excessive proliferation and migration of vascular smooth muscle cells are the key to pathogenic mechanism of diseases, so studying the effect of traditional Chinese medicine on VSMC is significant on prevention of atherosclerosis. This paper mainly reviews promoting phenotypic of traditional Chinese medicine on vsmc, inhibiting excessive proliferation and migration and its mechanism, basic animal experiment, also discusses the relationship of VSMC with endothelial cells and endothelial progenitor cells and macrophage. Research has showed that traditional Chinese medicine has good effect on vascular diseases, because of its complicated composition, difficult to definite its mechanism and to control its quality, and its side effect which limit its application in clinic. Therefore, combing with serum pharmacology and strengthening studying on active monomer compounds of traditional Chinese medicine may bring new hopes for AS.关键词：vascutar disease;vascular smooth muscle cell;atherosclerosis
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发布时间：2024-01-04 - 摘要：Objective: The chemical compositions and pharmacological activities of the Marsdenia tenacissima were reviewed and analyzied. Method: We search data on chemical compositions and pharmacological effects of M. tenacissima in recent years by the CAS Shanghai institute of materia database platform. Result: The chemical constituents of M. tenacissima is more complex,so the current research focuses on C21 steroidal glycosides. Its pharmacological research is now mainly in their antitumor effect.Now it has a variety of clinical drug which used M. tenacissima as main raw material for treatment of various cancers.But it still lack study which is the specific effective constituents of M. tenacissima and what is the mechanism, therefore, the chemical components and pharmacodynamics of M. tenacissima must be study. Conclusion: In recent years, study on anti-tumor effect of M.tenacissima has more reports, other pharmacological research,particularly the actions on the respiratory system, need to be strengthened, and this paper can provide reference for further research and development.
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发布时间：2024-01-04 - 摘要：Objective: To summarize the application progress of metabonomics in study of traditional Chinese medicine(TCM). Method: Literature about application of metabonomics in the study of TCM in recent years was reviewed. Result: Metabonomics could reveal the changes of metablites and metabolic pathway in the biological systems when using TCM to cure diseases. It laid stress on the studying the function level of organism in an overall perspective, which was in accord with the TCM theory of viewing human body as the unity and similar to its characteristics of multiple-components and targets and whole, combined effects also. It was widely used in the research of TCM syndromes, meridians and acupuncture, effect, mechanism, material basis and safety evaluation of TCM. When metabonomics was used to study TCM, it was important to raise further analysis and determination standards and to integrate useful analysis technology to make simultaneous detection of various chemicals coming true. Metabonomics database should be perfected also. It was vital to enhance the intergration of data processing and analysising to meet the high throughput and mass requirement in the study of metabonomics. Conclusion: Metabonomics has a vast potential for future application in the research of TCM.关键词：metabonomics;TCM symptoms;meridians and acupuncture;mechanism;safety evaluation traditional Chinese medicine
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发布时间：2024-01-04 - 摘要：Objective: The present review aimed to study the history and clinical application of Alpiniae Oxyphyllae Fructus in traditional Chinese medicine (TCM) in the past 2000 years. Method: We searched the TCM classics such as Ben Cao Tu Jing, Zheng Lei Ben Cao, Tang Ye Ben Cao, Shen Nong Ben Cao Jing, Wei Shi Jia Cang Fang and Dan Xi Xin Fa using the terms "pharmacognosy", "clinical application", and "combination therapy" corresponding to the Alpiniae Oxyphyllae Fructus. Result: The Alpiniae Oxyphyllae Fructus were mainly used in the compound formulae or fufang, for the treatment of the following disease: ① frequent Urination and urine dribbling, ② gynecologic diseases such as metrorrhagia and metrostaxis, ③ vomiting, ④ colic and others. Conclusion: This review will provide us the useful information for the modern research and clinical uses of Alpiniae Oxyphyllae Fructus.关键词：Alpiniae Oxyphyllae Fructus;history evolution;characters of application
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发布时间：2024-01-04 - 摘要：Objective: By referring to the relevant literatures, it has been summarized the processing research of Gardenia jasminoids roasted with ginger juice. Method: The review has mainly contained the evolution of the processing history, the records of the processing procedures, the situation of processing technology, the variations of the chemical constituents and the pharmacological actions after being processed of G. jasminoids roasted with ginger juice. Result: The processing method of G. jasminoids roasted with ginger juice, which has been recorded in the processing procedures of many provinces, was firstly described in (chanbao zalu). At present, it has not been well studied on the modern processing technology, chemical constituents and pharmacological actions. Conclusion: It is necessary to research on the chemical constituents and pharmacological actions of G.jasminoids roasted with ginger deeply and systematically to reveal its processing principle and standardize its processing technology.关键词：Gardenia jasminoids;Gardenia jasminoids roasted with ginger juice;processing;to roast with ginger juice
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发布时间：2024-01-04 - 摘要：Through review and analysis of literature in 30 recent years and investigating 78 238 copies of prescription from local hospitals intresent three years. It was found that compatibility of Flos Genkwa and Radix Glycytthizae was seldom applied in clinical application and there were more reports on experimental research. Literature reports limited in three kinds of diseases like miscarrying, chilblain, asthma and three prescription. Experimental studies concerned observing of clinical signs and symptoms and cytochromes enzymes and liquid chromato graphy. Ancient literature and modern experimental research can provide evidence for safe application in clinical practice.关键词：Flos Genkwa;Radix Glycytthizae;retroversion of literature;hospital of basic level;investigate
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发布时间：2024-01-04 - 摘要：Objective: To explore the rules of Chinese patent medicines, western medicines, and their combination rules in the treatment of type 2 diabetes through data mining. Method: We queried and downloaded the data on type 2 diabetes from Sino Med on March, 2011.By transferring semi-structured data structured database of Microsoft SQL was set into, we got the raw data for data mining. By executing the algorithm of discrete derivaties, we got some simple and meaningful networks of type 2 diabetes. In cytoscape, these networks were visualized. Result: In the treatment of type 2 diabetes, Chinese patent medicines, i.e., Xiaoke Wan, Salvia Miltiorrhiza Injection, Radix Astragali Injection, Liuweidihuang Wan, and Yuquan Wan were most frequently used. among all the western medicines, metformin, insulin pump, subcutaneous injection of insulin, rosiglitazone, repaglinide, gliclazide, glipizide, acarboseare, and pioglitazone are most concerned in the treatment of type 2 diabetes. Conclusion: Rules of Chinese patent medicines, west medicines, and their combinations in the treatment of type 2 diabetes are proposed in this paper.关键词：type 2 diabetes;text mining;Chinese patent medicine;western medicine;drug laws
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发布时间：2024-01-04 - 摘要：Objective : To investigate pathogenesis and efficacy of Fuyuan Huoxue decoction in the treatment of pain caused by pleural tumor, to alleviate the pleural tumor pain with effective methods of Chinese medicine treatment. Method :Li Dongyuan’s Fuyuan Huoxue decoction for the treatment of pain caused by pleural tumor was observed in patients,evaluated with NRS score, mental status changes and possible side effects. Result :The analgesic effect of the decoction was rapid, overall NRS score and mental state was improved apparently, no obvious side effects appared. Conclusion : Fuyuan Huoxue decoction could significantly improve patients’pain caused by pleural tumor lesions. While the effect of three ladder acesodyne medication is unsatisfactory, traditional Chinese medicine treatment can provide a effective way of analgesic therapy.关键词：cancer pain;Fuyuan Huoxue Tang;pleural tumor
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