最新刊期
卷 19 ， 期 21 ， 2013
- 摘要：Objective: To optimize matrix formulation of Jiufen cataplasms and investigate its in vitro release characteristics. Method: With viscosity and in vitro release constant of Jiufen cataplasms as indexes,effects of dosage of carbopol,CMC-Na,sodium polyacrylate,polyvinyl alcohol and glycerol on quality of Jiufen cataplasms were investigated by uniform design,multiple regression of test data was analyzed by Excel,then optimized matrix formulation of Jiufen cataplasms and determined its in vitro release.The content of strychnine was determined by HPLC,chromatographic conditions were as follows:Hypersil BDS C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-water-triethylamine-glacial acetic acid(20:280:0.3:2.4,pH 4.5),flow rate l mL·min-1,detection wavelength 254 nm. Result: The best matrix formulation of Jiufen cataplasms was:carbopol-polyacrylate sodium-polyvinyl alcohol-sodium carboxymethylcellulose-glycerol(1:1:1:1.75:20).In vitro release properties of Jiufen cataplasms conformed to Higuchi equation with R2 of 0.902 4. Conclusion: Jiufen cataplasms had good forming,it could maintenance in vitro release for 24 h.Method combining Excel software and uniform design was simple and feasible.关键词：Jiufen cataplasms;matrix formulation;uniform design;strychnine;in vitro release test
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare changes of chemical composition and efficacy before and after Phytolaccae Radix processing with vinegar. Method: Phytolaccae Radix was processed by according to vinegar processing method in the 2010 edition of ‘Chinese Pharmacopoeia’.The content of total saponins was dtermined by sulfuric acid-vanillin colorimetry;The content of esculentoside A was determined by HPLC-ELSD,chromatographic conditions were:Lichrospher-C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.4% acetic acid(65:35),flow rate 1.0 mL·min-1,column temperature 40 ℃,injection volume 20 μL;ELSD conditions were:drift tube temperature 90 ℃,flow rate of carrier gas 2.5 L·min-1.SD rats were randomly divided into normal saline group,positive drug group (hydrochlorothiazide),crude and processed Phytolaccae Radix group,brine loading model formed with saline,then we observed urine of rats within 5 hours by metabolic cage method.ICR mice were randomly divided into normal saline group,positive drug group (mirabilite),crude and processed Phytolaccae Radix group,then we compared small intestinal propulsive rate between crude and processed Phytolaccae Radix by ink propulsion method. Result: After processing of Phytolaccae Radix with vinegar,the content of total saponins increased from 1.66% to 1.91%,the content of esculentoside A increased from 0.64% to 0.82%,diuretic efficacy on rats enhanced and purgative efficacy on mice moderated. Conclusion: After being processed with vinegar,the content of total saponins and esculentoside A both increased,diuretic efficacy enhanced and purgative efficacy moderated.关键词：Phytolaccae Radix;processed with vinegar;total saponins;esculentoside A;diuretic;diarrhea;efficacy comparison
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a new technology of mechanical compression for Myristicae Semen into frostlike powder and compare with traditional compression process. Method: With oil yield and properties as indexes,single factor tests were adopted optimize mechanical compression process with compression times and temperature as factors.With the contents of fatty oil,volatile oil,total lignans and dehydrodiisoeugenol before and after pressing into frostlike powder,then investigate effect of mechanical compression and traditional compression into frost-like powder to chemical composition in Myristicae Semen.The content of dehydrodiisoeugenol was determined by HPLC,chromatographic conditions were as follows:Diamonsil C18 column(4.6 mm×250 mm,5 μm),column temperature 30 ℃,mobile phase of methanol-water gradient elution,detection wavelength 274 nm,flow rate 1.0 mL·min-1,injection volume 20 μL. Result: Optimized mechanical compression process was:compression temperature 80-90 ℃ with 2 times,oil yield 30.8%.Compared with raw products,the contents of fatty oil,volatile oil,total lignans,dehydrodiisoeugenol were all reduced after mechanical compression into frost-like powder,but it was similar to traditional compression into frost-like powder. Conclusion: Mechanical compression process was convenient,quick and suitable for industrial production,it could replace traditional compression method.关键词：Myristicae Semen;mechanical compression;traditional compression;quality evaluation;content determination
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Strychni Semen with vinegar. Method: Single factor tests and orthogonal test were optimized processing technology of Strychni Semen with vinegar,effects of kinds of vinegar,ratio of drug and liquid,vinegar soaking time and blanching time on the contents of brucine and strychnine were investigated.The contents of brucine and strychnine were determined by HPLC,chromatographic conditions were as follows:Elite Hypersil BDS C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water-diethylamine(34:100:1),detection wavelength 258 nm,flow rate 1 mL·min-1,column temperature 30 ℃. Result: The best processing technology of Strychni Semen with vinegar was:balsamic vinegar,proportion of drug and liquid(3:14),vinegar soaking time 1 d and blanching time1 d.The contents of brucine and strychnine were 0.131 5%,0.177 3%,respectively. Conclusion: Optimized processing technology was stable,feasible and reproducible,it could be used to reduce toxicity of Strychni Semen.关键词：vinegar;Strychni Semen;brucine;strychnine;orthogonal test
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate effects of temperature,time in processing process of Ginseng Radix et Rhizoma Rubra on the content of ginsenosides. Method: The content of total saponins was determined by UV,orthogonal test was adopted to investigated effects of heating time,steaming temperature,steaming time,drying temperature on total saponins content.The contents of ginsenoside Rb1,Rb2,Rc,Rd,Re,Rg1,Rg2,Rg3,Rh1,Rh2 were proformed by HPLC,chromatographic conditions were as follows:ZORBAX SB-C18 column(2.1 mm×100 mm,3.5 μm),column temperature 30 ℃,mobile phase of water-acetonitrile gradient elution,flow rate 1.0 mL·min-1,detection wavelength 203 nm,injection volume 5 μL;Effects of different steaming and drying temperature on kinds of ginsenosides were compared by high resolution liquid chromatography-quadrupole-time of flight mass spectrometry. Result: Optimum processing technology for the highest content of total saponins was:temperature raised to 100 ℃ at 60 min,steamed for 6 h and dried at 50 ℃,steaming and drying temperature had significant difference.Under different steaming and drying temperature,the contents of ginsenoside Rb1,Rb2,Rc,Rd,Re,Rg1,Rg2,Rg3,Rh1,Rh2 changed remarkably. Conclusion: According to needs of actual production,processing method of Ginseng Radix et Rhizoma Rubra should adjust for quality control.This study would provide a reference for production and new drugs development of ginsenosides.关键词：Ginseng Radix et Rhizoma Rubra;ginsenosides;steaming temperature;orthogonal test;high resolution liquid chromatography-quadrupole-time of flight mass spectrometry
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total phenolic acids from Crepis turczaniowii. Method: Based on single factor tests,central composite design-response surface methodology was adopted to optimize extraction process of total phenolic acids from C. turczaniowii,independent variables were ethanol concentration,reflux time and solvent folds,dependent variable was extraction rate of total phenolic acids.Linear or no-linear mathematic models were used to estimate relationship between independent and dependent variables,optimum extraction process was optimized,then prediction was carried out through comparing the observed and predicted values.The content of total phenolic acids was determined by UV. Result: Optimum extraction technology conditions were:extracted 3 times with 35 times the amount of 70% ethanol for 55 minutes each time.Regression coefficient of binomial fitting complex model was as high as 0.936 9,bias between the observed and predicted values was -2.28%. Conclusion: Optimum extraction process was reasonable,stable and feasible,it was suitable for development and utilization of total phenolic acids from C. turczaniowii.关键词：Crepis turczaniowii;single factor test;central composite design-response surface methodology;total phenolic acids
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To prepare olive chewable tablets and investigate its antioxidant capacity. Method: With flavor,appearance,taste and hardness as composite index,orthogonal test was adopted to optimized formulation technology of olive chewable tablets by taking dosage of lactose,magnesium stearate,mannitol and PVP-k30 as factors.The content of polyphenols from olive chewable tablets was determined by HPLC,chromatographic conditions were:C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.085% phosphoric acid(8:92),detection wavelength 280 nm,flow rate 1 mL·min-1.Antioxidant capacity of olive chewable tablets was evaluated by investigating its scavenging DPPH free radicals. Result: Optimal formulation technology was as following:33% raw material medicine,10% lactose,35% mannitol,10% PVP-K30,1% magnesium stearate,each tablet (0.38 g) containing gallic acid of 1.64 mg,ability of olive chewable tablets to scavenging DDPH free radicals was better than Vitamin C. Conclusion: These prepared olive chewable tablets had antioxidant health function with good taste,it could provide some certain ideas on protection and development of geoherbs in the South China.关键词：olive;chewable tablets;gallic acid;1,1-diphenyl picrylhydrazyl;antioxidant activity
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of gallic acid in Acalypha australis from Guizhou. Method: The content of gallic acid from A. australis was determined by HPLC,Diamonsil C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.05% phosphoric acid(4:96),detection wavelength 270 nm,column temperature at room temperature,flow rate 1 mL·min-1,injection volume 10 μL.With transfer rate of gallic acid as index,single factor tests were adopted to screen extraction methods and solvents,effects of ratio of solid-liquid,ethanol concentration,extraction temperature and time on extraction technology were investigated by orthogonal test. Result: The best extraction process of gallic acid was as following:extracted 2 times with 30 times the amount of 70% ethanol at 90 ℃,4 hours per time.Average transfer rate of gallic acid was 7.888 mg·g-1(RSD 1.60%). Conclusion: This optimized extraction process was stable and feasible,it could provide experimental basis for development and utilization of A. australis from Guizhou.关键词：Acalypha australis;HPLC;gallic acid;orthogonal test;extraction process;single factor test
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate feasibility of fresh-cut process for Inulae Radix and improve traditional slicing process of it. Method: The contents of alantolactone,isoalantolactone and total flavonoids were employed as indicators,effects of water content,drying styles and slice thickness on fresh-cut process of Inulae Radix were investigated by orthogonal test,then compared with conventional slicing process.The content of total flavonoids was determined by UV;The contents of alantolactone and isoalantolactone were determined by HPLC,chromatographic conditions were as follows:SHIMADZU C18 column(4.6 mm×200 mm,5 μm),mobile phase of acetonitrile-0.1% phosphoric acid(50:50),column temperature 30 ℃,flow rate 1.0 mL·min-1,detection wavelength 220 nm,injection volume 20 μL. Result: Optimized fresh-cut process was:water content 35%,drying styles of shade drying,slice thickness 2.5-3.0 mm.The contents of alantolactone,isoalantolactone and total flavonoids from fresh-cut pieces were (22.41±1.38),(12.87±0.73),(15.04±0.64) mg·g-1,but in traditional slicing process were (22.94±1.24),(13.24±0.83),(14.12±0.58) mg·g-1. Conclusion: Fresh-cut process of Inulae Radix was stable and meaningful.关键词：Inulae Radix;fresh-cut;alantolactone;isoalantolactone;total flavonoids;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize in vitro stomach bionic transformation process of Schisandrae Chinensis Fructus. Method: With the cream rate and the content of schisandrin as indexes,orthogonal test and single factor tests were adopted to investigate effects of the concentration of Schisandrae Chinensis Fructus decoction,volume ratio of decoction and artificial gastric juice,transformation time,transformation times,speed and temperature on in vitro stomach bionic transformation process.The content of schisandrin was determined by HPLC. Result: Optimun process was as following:decoction concentration 1.0 g·mL-1,volume ratio of decoction and artificial gastric juice (1:1),temperature 37 ℃,speed 75 r·min-1,transformation time 1 h,conversion of 1 times. Conclusion: Optimized in vitro stomach bionic transformation process was stable and reliable,which established experimental basis for human metabolism transformation of Schisandrae Chinensis Fructus.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate effects of high pressure processing on contents of emodin,physcion and 2,3,5,4'-stilbene glucoside from Polygoni Multiflori Radix. Method: Polygoni Multiflori Radix was processed by high pressure steaming method,effects of different steaming time on contents of anthraquinones and 2,3,5,4'-stilbene glucoside were investigated,and properties change during processing was observed.The contents of emodin and physcion were determined by HPLC,chromatographic conditions were as follows:Diamonsil C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.1% phosphoric acid(80:20),flow rate 1.0 mL·min-1,detection wavelength 254 nm,column temperature 30 ℃,injection volume 10 μL.HPLC conditions of 2,3,5,4'-stilbene glucoside were as follows:mobile phase of acetonitrile-water gradient elution,detection wavelength 320 nm;other conditions were the same as these above conditions. Result: The content of 2,3,5,4'-stilbene glucoside were 4.55%,3.54%,2.20%,1.87%,1.52%,1.15%,0.76% after high pressure steamed 0,1,2,3,4,5,6 h.By increasing of steaming time,contents of incorporating anthraquinones and 2,3,5,4'-stilbene glucoside from Polygoni Multiflori Radix gradually decreased,but the content of free anthraquinones increased. Conclusion: As tonics applications,Polygoni Multiflori Radix should be heated for four hours under high pressure.关键词：Polygoni Multiflori Radix;Polygoni Multiflori Radix Praeparata;processing;high pressure steaming;2,3,5,4’-stilbene glucoside
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro percutaneous permeability of five drug components in Guanxin paste gel ointments. Method: Improved Franz cell was used for transdermal penetration test with physiological saline solution as absorption solution and SD rat skin as experimental skin.The cumulative release amount and cumulative osmotic amount of danshensu,ferulic acid,ligustrazine hydrochloride,cryptotanshinone and tanshinoneⅡA were measured by HPLC in order to evaluate their percutaneous permeability,chromatographic conditions were as follows:Kromasil C18 column(4.6 mm×250 mm,5 μm),detection wavelength 280 nm,flow rate 1.0 mL·min-1,mobile phase of methanol-0.5% aqueous acetic acid with binary linear gradient elution,column temperature at room temperature,injection volume 10 μL. Result: The cumulative release quantity and cumulative osmotic amount of these five components in Guanxin paste gel ointments were in order of ferulic acid > danshensu > ligustrazine hydrochloride > cryptotanshinone > tanshinone ⅡA.The percentage of cumulative relative permeability via skin of five components within 24 h were 73.09,82.62,82.89,37.22,24.80,respectively.The cumulative release and cumulative percutaneous permeability of these five components conformed to Higuchi equation with correlation coefficient of 0.999 9. Conclusion: Five components in Guanxin paste gel ointments including danshensu,ferulic acid,ligustrazine hydrochloride,cryptotanshinone and tanshinone ⅡA had good drug release properties and percutaneous permeability.关键词：gel ointments;danshensu;ferulic acid;ligustrazine hydrochloride;Cryptotanshinone;Tanshinone ⅡA;percutaneous penetration
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize prescription of total saponins from leaves of Notoginseng Radix et Rhizoma liposomal gels and investigate its in vitro transdermal absorption properties. Method: Total saponins from leaves of Notoginseng Radix et Rhizoma liposomes were prepared by thin-film dispersion Method,with encapsulation efficiency as index,orthogonal test was adopted to optimize liposomes formulation by taking ratio of phospholipids-cholesterol,lipids-drug ratio and drug concentration as factors.High-speed centrifugation method was used to determine encapsulation efficiency,carbomer 940 was added as a substrate for preparation of gels,its dosage was investigated by single factor test.In vitro transdermal test was adopted to investigate in vitro transdermal absorption of ginsenoside Rb3 from total saponins in leaves of Notoginseng Radix et Rhizoma,the content of ginsenoside Rb3 was determined by HPLC,chromatographic conditions were as follows:ZORBOX SB-C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile(A)-0.2% phosphoric acid(B) gradient elution(0-19 min,30% A;19-21 min,35% A;21-26 min,50% A),detection wavelength 203 nm. Result: Optimized formulation technology was:lecithin-cholesterol(4:1),the concentration of ginsenoside Rb3 90 g·L-1, lipid-drugs(6:1),the highest cumulative permeation rate of drug through skin when the content of carbomer 940 was 0.5%. Conclusion: Liposomal gels could increase permeation amount of active ingredients in skin,this study laid experimental foundation for development of new dosage forms and new administration routes.关键词：total saponins from leaves of Notoginseng Radix et Rhizoma;thin-film dispersion method;entrapment efficiency;liposomes;gels;transdermal absorption;determination
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To prepare bufotoxin microemulsion and investigate its physicochemical properties and in vitro penetration characteristics. Method: Bufotoxin microemulsion prescription was screened by solubility tests and pseudo-ternary phase diagrams;Morphology,particle size,Zeta potential and stability of microemulsion was investigated;In vitro transdermal process of bufotoxin microemulsion was investigated by transdermal diffusion instrument.The content of bufotoxin was determined by RP-HPLC,chromatographic conditions were as follows:ZORBAX SB-C18 column(4.6 mm×150 mm,5 μm),mobile phase of methanol-water(70:30),flow rate 1.0 mL·min-1,column temperature 30 ℃,detection wavelength 300 nm,injection volume 10 μL. Result: Optimal microemulsion formulation consists of GTCC-Cremophor RH 40-Transcutol HP-water(10.47%:33.69%:11.23%:44.61%),drug loading of (1.36±0.12)%.Appearance of bufotoxin microemulsion was round,average particle size,PDI and Zeta potential were (31.33±5.362) nm,(0.103±0.025) and-(30.85±3.25) mV,respectively.Cumulative amount and skin retention amount of bufotoxin microemulsion in 12 h were 1 339.32 μg·cm-2 and 1 895.36 μg,which was 3.96 and 4.89 folds higher than that of bufotoxin saturated solution,respectively. Conclusion: Bufotoxin microemulsion was stable,it could significantly promote transdermal absorption of bufotoxin.关键词：bufotoxin;microemulsion;transdermal delivery;melanoma;pseudo-ternary phase diagram;stability investigation
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare in vitro dissolution of jasminoidin between ultrafine powder and fine powder of Gardeniae Fructus. Method: Paddle method was adopted in vitro dissolution test.HPLC was developed for determining dissolution of jasminoidin from ultrafine powder and fine powder of Gardeniae Fructus in different periods,then calculated accumulative dissolution,chromatographic conditions were:Wondasil C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(15:85),flow rate 1.0 mL·min-1,detection wavelength 238 nm,column temperature 30 ℃,injection volume 10 μL. Result: Accumulative dissolution of jasminoidin from ultrafine powder and fine powder of Gardeniae Fructus were 92.82%,83.36%,T0.8 were 9.92,26.35 min,respectively.Dissolution and dissolution rate of Gardeniae Fructus ultrafine powder were all higher than fine powder. Conclusion: Micronization of Gardeniae Fructus contributed to dissolution of effective components.关键词：Gardeniae Fructus;jasminoidin;dissolution;ultrafine powder;fine powder;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Akebia trifoliata.Method: The compounds were separated and purified by column chromatography and their structures were established by spectroscopic methods. Result: Fifteen compounds were isolated from the extract of A. trifoliata, whose structures were determined as 3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin(1), 3-O-β-D-xylopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin(2),3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin(3), 3-O-α-L-arabinopyranosidehederagenin(4), 3-O-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl-28-O-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside hederagenin(5), 3-O-([β-D-glucopyranosyl(1→4)]-[α-L-rhamnopyranosyl-(1→2)]-α-L-arabinopyranosyl)hederagenin(6),3-O-α-L-rhamnopyranosyl(1→2)-[β-D-glucopyranosyl(1→4)]-α-L-arabinopyranosyl-28-O-α-L-rhamnopy-ranosyl-(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyra-nosidehederagenin(7), 28-O-β-D-xylopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→2)-β-D- glucopyranosyl (1→6)-β-D-glucopyranoside arjunolic acid (8), 28-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl (1→6)-β-D-glucopyranoside 30-no arjunolic acid (9), 3-O-α-L-rhamnopyranosyl(1→2)-α-Larabinopyranosyl-28-O-β-D-glucopyranosi-de hederagenin (10). Conclusion: Compounds 8,9 were isolated from this plant for the first time and 10 was isolated from genus acanthopanax for the first time.关键词：Akebia trifoliata;chemical components;separation and identification
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate into lignans of active components of Saururus chinensis anti-nicotine withdrawal symptoms.Method: Various column chromatography were used in the isolation and purification, and physiochemical constant determination and spectral analysis were adopted to determine the chemical structures of lignans.Result: Thirteen chemical compounds were isolated from the active of anti-withdrawal symptoms,sauchinone (1),sauchinone A (2),1'-epi-sauchinone (3),licarin B (4),licarin A (5),5-methoxy-licarin A (6),saucernetin (7),5,5'-dimethoxy-saucernetin (8),nectandrin B (9),5,5'-dimethoxy-nectandrin B(10),3',4'-methylenedioxy-3,4,5,5'-tetramethoxy-7,7'-epoxylignan(11),3',4'-methylenedioxy-3,4,5-trimethoxy-7,7'-epoxylignan (12),machilin D (13). Conclusion: Compounds 6, 8, 10-12 were first isolated from this plant.关键词：Saururus chinensis;lignans;chemical components
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Investigation on RP-HPLC-ELSD fingerprint of triterpenes in Poria cocos from different origin place, in order to provide a theory evidence to evaluate the quality of P. cocos from different origin place, and improve the quality of P. cocos. Method: The chromatographic conditions were Kromasil 100-5 C18 column (4.6 mm×250 mm, 5 μm), 0.2% methanoic acid (A) and acetonitrile (B) as gradient mobile phases, flow rate being 1.0 mL·min-1, column temperature at 30 ℃, evaporative light-detection conditions:atomization temperature at 40 ℃, pressure of 350 kPa, gain value of 7, and 20 μL sample injection. Result: Fingerprint was established with 18 common peaks and methodology reached the required standard. Conclusion: The method is simple, rapid, accurate, and can be used as an effective means of P. cocos quality evaluation of different origin place.关键词：origin place;Poria cocos;triterpenes;ELSD-RP-HPLC;fingerprint
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Morinda officinalis.Method: The compounds were isolated and purified by recrystallization and column chromatography with silica gel.Their structures were elucidated on the basis of physicochemical properties and spectral analysis.Result: Nine compounds were identified as β-quebrachol(1),rubiadin -1- methyl ether(2)rubiadin(3),2-hydroxy-3-methylanthraquinon(4),1-hydroxy-2-hydroxymethylanthraquione (5),2-hydroxy-1-methoxyanthraquione(6),dimethylhexyl phthalate(7), 3β,19α-dihydroxyl-12-en-28-oic acid(8),monotropein(9)respectively.Conclusion: Compound 7,8 were isolated from M. officinalis for the first time.关键词：Morinda officinalis;chemical constituents;structure identification
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To synthesis 4'-N,N-Bis-substituted (aminomethyl) benzoate ether derivatives of scutellarein with improved anti-oxidative activity, and investigation of their protection against nerve cells oxidative damage. Method: Scutellarein 2-bromoethyl ether (4) was synthesized in the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) with scutellarein and 2-bromoethanol as starting materials. N-substituted (aminomethyl) benzoic acids (5a-c) were converted to benzoic chloride (6a-c) by using oxalyl chloride, then the latter coupled with 4 in the presence of 4 MS to obtained compounds (7a-f), then diphenyl ketal protecting group of the above compounds was removed by using acetic acid chloride/methanol system to obtain target compounds(8a-f), prodrugs were tested for their cytotoxicity and antioxidant activity in PC12 cells by MTT and LDH leakage rate assay. Result: The structure of synthesized compounds 8a-f was confirmed by the methods of 1H-NMR, ESI-MS. The results indicated that compound 8e and 8f have more potent anti-oxidative activity than positive control Vitamin E. Conclusion: The synthetic method we used has preferable practicality and can be used in synthesizing scutellarein 4'-N,N-bissubstituted (aminomethyl) benzoate ether derivatives, bioactivity evaluation results suggested that the kind of prodrugs are worthy of further investigation to discover new anti-oxidative agents.关键词：scutellarein;(aminomethyl) benzoic acids;prodrug;PC12 cell
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Viola yedoensis and their antioxidant activity.Method: A systematic isolation was performed by various column chromatography with the n-butanol fraction of the ethanolic extract of the herb. Result: Eleven compounds were isolated and identified as kaempferol-3-O-β-D-sophorosyl-7-O-α-L-rhamnopyrano-side (1), kaempferol-3,7-di-O-α-L-rhamnopyranoside (2), apigenin-6,8-di-C-β-D-glucopyranoside (3), adenosine (4), esculetin (5), apigenin-6-C-α-L-arabinopyranosyl-8-C-β-D-xylopyranoside (6), apigenin-6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside (7),apigenin-6-C-β-D-glucopyranosyl-8-C-β-D-xylopyranoside (8), apigenin-6,8-di-C-α-L-arabinopyranoside (9), apigenin-6-C-β-D-glucopyranosyl-8-C-β-L-arabinopyranoside (10) and kaempferol-3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopy-ranoside (11), respectively. All compounds were evaluated for antioxidant capacity against DPPH radicals, and compounds 1-5 and 11 showed antioxidant activities. Conclusion: Compound 2 was reported from Viola yedoensis for the first time, and compounds 1 and 8 were the first time to be isolated from the genus Viola.关键词：Viola yedoensis;chemical constituents;antioxidant activity;DPPH
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Radix Gentiana crassicaulis is the major cultivated Gentiana species in Yunnan Province. Wildlife tending is a major cropping pattern. In this study, an improved and comprehensive HPLC fingerprints were developed for the quality evaluation of G. crassicaulis, together with the simultaneous determination of four iridoid and secoiridoid glycosides. Loganic acid, swertiamarin, gentiopicroside, sweroside were also detected. Method: HPLC separation was performed on an Agilent Zorbax SB C18 column. The mobile phase was methanol and water with linear gradient change of methanol from 0 to 40% in 20 min. The flow rate was 1.0 mL·min-1, and the detection wavelength was set at 238 nm. Result: The HPLC chromatograms of fifteen samples from different regions of China showed a similar pattern. Thirteen peaks were selected as the characteristic peaks to evaluate the similarities among different G. crassicaulis samples. Conclusion: This is the first report on an HPLC method for the simultaneous determination of four bioactive components and fingerprint analysis of wildlife tending G. crassicaulis. The results indicate that this comprehensive method is suitable for evaluate the quality of G. crassicaulis for GAP planting.关键词：Gentiana crassicaulis;wildlife tending;HPLC;fingerprint;quality evaluation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for the simultaneous determination of seven major compound in Xiaoaiping injection. Method: The analytical column was Kromasil 100-5C18(4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile-0.2% phosphoric acid, gradient elution. The flow was 0.8 mL·min-1. The detection wavelength was 300 nm. The column temperature was 30 ℃. Result: The linear ranges of seven compounds were as fllows:0.1-3.2 mg·L-1 (r=0.999 3)for neochlorogenic acid,0.025-0.8 mg·L-1(r=0.999 9)for protocatechuic aldehyde,0.1-3.2 mg·L-1(r=0.999 8) for chlorogenic acid,0.075-2.4 mg·L-1(r=0.999 9) for 4-dicaffeoylquinic acid,0.037 5-1.2 mg·L-1(r=0.999 9) for vanillic acid,0.02-0.64 mg·L-1(r=0.999 9) for caffeic acid,0.01-0.32 mg·L-1(r=0.999 9)for 4-coumararate.The average recoveries were 99.6% (RSD 0.24%),100.0% (RSD 0.15%),98.7% (RSD 1.00%),99.1% (RSD 1.36%),96.4% (RSD 1.37%),98.3% (RSD 1.80%),97.3% (RSD 1.64%) respectively. Conclusion: This method is simple, accurate and available for the quality control of Xiaoaiping Injection.关键词：Xiaoaiping injection;neochlorogenic acid;protocatechuic aldehyde;chlorogenic acid;4-dicaffeoylquinic acid;vanillic acid;caffeic acid;4-coumararate
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Todeterminethe optimal harvesting time andinitial processing method for Polygonum capitatum herbs from GAP cultivationbase, in order to guide the harvesting and processing of P.capitatum. Method: The content of quercetin, quercitrin, gallic acid and water-soluble extracts fromP.capitatum herbs with different harvesting time, different initial processing for GAP cultivation baseand water-soluble extract was determined by HPLC, and the determination results were assessed by principal component analysis. Result: The synthetic graded value of P.capitatumherbs in later Augustwas 2.093 0, dry in the shadewas 1.723 1.They include the best comprehensive information for the four kinds of index components, and it can be determined that the optimal harvesting time of P.capitatumwas in later August and the best initial processing method of P.capitatum is dry in the shade. Conclusion: The accumulation of the index component from P.capitatum in different periods of growth is regularly, the processing method has a great influence on P.capitatum.关键词：Polygonum capitatum;principal component analysis;harvesting time;initial processing method
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard for Yangxinanshen pills. Method: Fructus Schisandrae Chinensis, Salvia miltrorrhiza and Caulis Polygoni Multiflori in Yangxinanshen pills were identified by TLC. The deoxyschizandrin and schisandrin B were determined by HPLC. Result: By TLC, the spots of three herbal drugs were well separated and without interference. Deoxyschizandrin showed a good linear relationship in the range of 0.242-4.84 μg (r=0.999 2) and the average recovery was 98.48% (RSD 1.03%). Schisandrin B showed a good linear relationship in the range of 0.110-2.21 μg (r=0.999 4) by HPLC. The average recovery was 97.10% (RSD 1.03%). Conclusion: The method is simple, reliable and accurate, which can be applied to quality control of Yangxinanshen pills.关键词：Yangxinanshen pills;TLC;HPLC;quality standard
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect of the processing methods of the heating and the steaming on the content of six ester-type alkaloids in Radix Aconite Lateralis. Method: By using the processing methods of the high temperature-heating and the high pressure-steaming to control the temperature and time, six ester-type alkaloids in the different processed products of Radix Aconite Lateralis were determined. Result: There is a significant difference on the content of six ester-type alkaloids between the crude and the processed Radix Aconite Lateralis. It is significantly reduced or disappeared on the content of diester-type alkaloids and increased on monoester-type alkaloids. The linear ranges of benzoylmesaconine, benzoylaconine, benzoylhypaconine, mesaconitine, hypaconitine and aconitine were 0.032 184-3.218 4 μg (r=0.999 9), 0.030 016-3.001 6 μg (r=0.999 9), 0.031 320-3.132 0 μg (r=0.999 9), 0.030 744-3.074 4 μg (r=0.999 9), 0.030 912-3.091 2 μg (r=0.999 9), 0.031 920-3.192 0 μg (r=0.999 9) and the average recoveries were 100.03%, 99.99%, 98.16%, 100.68%, 99.27%, 102.81% and RSDs were 1.35%, 1.96%, 1.01%, 1.03%, 0.55%, 0.91% respectively. Conclusion: These processing methods are simple and controllable and can provide new references on processing radix aconite lateralis.关键词：Radix Aconite Lateralis;processing;ester-type alkaloids;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the difference of material base after vinegar processing by establishing the fingerprint of Fructus Schisandrae Chinensis and its processed products. Method: The chromatographic separation was performed at 35 ℃, with the acetonitrile-15 mmol·L-1 monopotassium phosphate(pH 2.0) as the mobile phrase in gradient elution.The detection wavelength was set at 254 nm and 210 nm, the flow rate was 1.0 mL·min-1. Result: There are significant differences after vinegar processing, in which 5-hydroxymethyl furfural content(5-HMF) in the most significant change. Conclusion: The established method can better reflect the differences of material foundation before and after processing, provides the scientific basis for quality evaluation of Schisandrae standards, and lays the foundation for further revealing the scientific connotation of the principle of Schisandra.关键词：Fructus Schisandrae Chinensis;fingerprint;vinegar processing
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To develop a method for determination of the main components elements in mineral drugs such as pearl powder, calamine and weathered sodium sulfate. Method: Calcium in pearl powder, zinc in calamine and sodium in weathered sodium sulfate were determined by ICP-OES after the samples were digested by microwave digestion system. Result: Calcium, zinc and sodium have a good liner in 0-20 mg·L-1 with the correlation coefficients 0.999 9-1.000 0, The RSD of repeatability for calcium, zinc and sodium were 1.4%,1.5% and 0.8%, and the recoveries were 105.0%, 101.9% and 104.5%. The results matched the results of flame atomic absorption spectrometry. Conclusion: The method was simple, sensitive, and reliable.It can be applied in the determination of main components elements in pearl powder, calamine and weathered sodium sulfate and their componets.关键词：ICP-OES;pearl powder;calamine;weathered sodium sulfate;elements
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To set up the quality standards of Dioscorea zingiberensis in Henan area preliminary. Method: On the basis of discrimination in Characters, microscopic and TLC, the content of water, total ash, acid insoluble ash and alcohol soluble extractive were determined According to the method in the Chinese Pharmacopoeia (edition 2010), and the content of diosgenin and pseudoprodioscin was determined by HPLC. Result: The moisture in Dioscorea zingiberensis should be no more than 7%;the total ash should be within 8%;the acid-insoluble ash should not be higher than 2%;alcohol soluble extractive should not be lower than 16%. The content of diosgenin should not be lower than 1%, and the content of pseudoprodioscin should be higher than 0.4%. Conclusion: The study provide the reference basis for making and perfecting the quality standards system of D. zingiberensis.关键词：Dioscorea zingiberensis;quality standard;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different chromogenic agents on determining the content of total saponins from Achyranthis Bidentatae Radix by UV spectrophotometry. Method: Total saponins were determined by UV with five different chromogenic agents namely method A as 5% vanillin-glacial acetic acid:perchloric acid:glacial acetic acid(2:8:50),method B as 5% vanillin-glacial acetic acid:perchloric acid:ethyl acetate(2:8:50),method C as perchloric acid,method D as sulfuric acid:methanol(7:3),method E as vanillin-sulfuric acid(2:58).With absorbance as index,chromogenic time and temperature was investigated by single factor tests. Result: Methdod C and D had not the maximun absorption at 400-800 nm;The maximum absorption wavelength of method E was at 544 nm,but absorbance value was too small which was not suitable for determination of standard curve;The maximum absorption wavelengths of method A and B were at (548±1),(554±1) nm,respectively,these two methods were suitable for determination of total saponins from Achyranthis Bidentatae Radix,whereas peak pattern and sample recovery of method A were better than method B. Conclusion: Method A was optimal chromogenic method of total saponins from Achyranthis Bidentatae Radix.关键词：Achyranthis Bidentatae Radix;total saponins;chromogenic agents;UV spectrophotometry;single factor test
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a quality control method of Feire Qingjie oral liquid with chlorogenic acid as index. Method: Mori Cortex and Phragmitis Rhizoma from Feire Qingjie oral liquid were identified qualitatively by TLC.HPLC was adopted to determine the content of chlorogenic acid from Feire Qingjie oral liquid,chromatographic conditions included Haito Pack ODS column(4.6 mm×250 mm,5 μm),methanol-1% glacial acetic acid(1:4) as mobile phase,detection wavelength at 326 nm,flow rate 1.0 mL·min-1,injection volume 10 μL,column temperature at 30 ℃. Result: By comparing with reference drug chromatography,Mori Cortex and Phragmitis Rhizoma showed the same spots on corresponding position without interference of negative control.Chlorogenic acid showed a good linear relationship in the rang of 3.180-106.0 mg·L-1 with r=1,average recovery was 100.5% with RSD 2.05%,the average mass concentration of chlorogenic acid from four batches of samples were 28.9,42.5,28.6,21.7 mg·L-1. Conclusion: This established method was convenient,quick,accurate and available for one of quality control methods for Feire Qingjie oral liquid.关键词：Feire Qingjie oral liquid;TLC;HPLC;Mori Cortex;Phragmitis Rhizoma
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To identify Ginseng and Quinquefolius by analysis of their volatile components; Method: Ginseng and Quinquefolius volatile components were extracted by Soxhlet, Steam distillation, SPME, Purge and trap extraction,methods, separated and detected by GC -MS, analyzed by searching NIST library and KI value. Result: There are obvious difference in type and content of volatile compounds in Ginseng and Quinquefolius using four different extraction methods. 42 (relative amount 69%) and 28 (41%) terpenoid components were identified in Ginseng samples and Quinquefolius samples by steam distillation respectively; 26 (50%) and 15 (18%) terpenoid components were identified in Ginseng samples and Quinquefolius samples by Soxhlet; 18(49%) and 5 (46%) terpenoid components were identified in Ginseng samples and Quinquefolius samples by SPME respectively; 2 (4%) and 4 (13%) terpenoid components were identified in Ginseng samples, and Quinquefolius samples by purge and trap extraction Conclusion: Characteristic volatile components in Ginseng and Quinquefolius are different under the same extraction conditions. We could use these properties to distinguish between the two herbs.关键词：Ginseng;Quinquefolius;volatile compounds;GC-MS;KI;Determination
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a specific TLC method and HPLC fingerprint for the quality evaluation of Ilex asprella. Method: TLC was performed with silica gel of Merck, using CHCl3-methanol-EtOAc-water-formic acid (20:20:40:10:2) as the developing solvent system. After sprayed with 10% H2SO4 ethanolic solution, the plate was heated at 105 ℃ and then observed under UV light at 366 nm. HPLC was performed on a Phenomenex Synergi Fusion C18column (4.6 mm×250 mm, 4 μm) with the column temperature at 25 ℃. The mobile phase consisting of 0.05% phosphoric acid and acetonitrile was used in gradient elution, with a flow rate of 1.0 mL·min-1. The detection wavelength was set at 203 nm. Result: I. asprella and its adulterants were differentiable for distinctive spots were observed on the TLC spectrum. The similarities of 15 bathes of I. asprella were between 0.716-0.890, while that of its adulterants were all below 0.630, calculated with Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004 A). Conclusion: Both the TLC method and HPLC fingerprint established in this study were suitable for discrimination among I. asprella and its adulterants, providing a reliable reference for qualitative evaluation of the medicinal material I. asprella.关键词：Ilex asprella;TLC;identification;HPLC;fingerprint
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: A reversed phase high performance liquid chromatography (RP-HPLC) separation coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used for the screening of multiple antioxidant compounds and the antioxidant basis of grape wine. Method: The red grape wine was passed through HPLC to enter into the PEEK coil contained the DPPH working solution, and signals were recorded at 517 nm. The Trolox equivalent antioxidant capacities (TEAC) were determined to compare the total antioxidant capacity and the antioxidant activity of the active compounds among the seven grape wines. Result: By qualitative analysis, gallic acid, protocatechuic, procyanin B1, gentisic acid, catechin, caffeic acid and ethyl gallate were active ingredients, but procyanin B2, vanillic acid, syringic acid, p-coumaric acid did not have the screening activity. The total antioxidant capacity and activity compositions were different among the seven grape wines, and the activity of procyanin B1, gallic acid, catechin and the unknown were better than caffeic acid, protocatechuic and ethyl gallate. Conclusion: Grape wines contain some amount of antioxidant compounds, and the on-line HPLC-DPPH assay, which is on-line, no-loss, high-flux and fast analysis, can be a powerful technique for screening analysis of antioxidant compounds in grape wine.关键词：grape wine;high performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl (HPLC-DPPH);antioxidant activity;on-line screening
- 0
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Analysis of Hubei Wufeng producing Chrysanthemum indicum volatile chemical composition. Method: The volatile chemical composition in Hubei Wufeng producing C. indicum was extracted by steam distillation, and then the composition was analyzed by GC-MS. Chromatographic peak area normalization method was used to calculate the relative mass fraction of each ingredient. Result: The results showed that 81 kinds of ingredients were isolated from C.indicum, 71 kinds were identified, and 9 unique kinds were identified in comparison with the other four regions. L-camphor was highest content, which was up to 5.840%, followed by caryophyllene oxide 5.807%,(-)- bornyl acetate 5.551%, oleic acid 4.873% and endo-borneol 4.668%. Conclusion: There is a significant difference in volatile chemical composition and content between Hubei Wufeng producing C.indicum and other regions.关键词：Chrysanthemum indicum;volatile chemical composition;L-camphor;GC-MS
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the quantitative method in the form of fingerprint peaks of Galla chinensis. Method: The fingerprints of G. chinensis were obtained, the peaks which can be separated from the baseline were defined as fingerprint peaks, and the peak of gallic acid was appointed as the benchmark peak. All the fingerprint peaks were quantified grounded on the peak of gallic acid. Result: Fourteen peaks were defined and quantified rested on the peak of gallic acid. Conclusion: A multicomponent quantitative method for G. chinensis is established. The method is feasible.关键词：Galla Chinensis;fingerprint;quantitative method
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the analytical method of quality control for the four reference ingredients in anti-radiation pneumonia decoction (ARPD). Method: Herbaceous peony glucoside, ferulic acid, coffee acid and glycyrrhizic acid were adopted as the reference substances. HPLC method was established using SHIMADZU LC-20AD HPLC system. Kromasil C18 column (4.6 mm×250 mm, 5 μm) was used at 30 ℃. Mobile phase consisted of acetonitrile-0.1% phosphoric acid was used for gradient elution at a flow rate of 1.0 mL·min-1. Result: The contents of herbaceous peony glucoside, ferulic acid, coffee acid and glycyrrhizic acid in ARPD refined extract were 522, 13, 12, 143 mg·L-1, respectively. Conclusion: The stability,accuracy and recovery of the methods are good and can be used for the quality control of ARPD refined extract.关键词：anti-radiation pneumonia decoction;high performance liquid chromatography;reference ingredient
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC-ELSD method for determination of the content of pinocembrin, cardamonin, sipeimine and sipeimine-3β-D-glucoside in Houzao Ninhuangsan(HZNHS). Method: An Hypersil C18 column was used as the chromatographic column, the flow rate was 0.9 mL·min-1. The mobile phase for pinocembrin and cardamonin consisted of methanol (A) and 0.5% phosphoric acid solution (B);the UV detection wavelength was at 300 nm.The mobile phase for sipeimine and sipeimine-3β-D-glucoside consisted of acetonitrile-water-diethylamine (65:35:0.5). The temperature of drift tube was set at 80 ℃, and the gas flow (N2) was set at 2.3 SLPM·min-1. Result: There was a good linear relationship between the concentration of pinocembrin, cardamonin and peak area value when the concentrations of pinocembrin and cardamonin were within the range of 0.052 7-1.054 μg (r=0.999 4), 0.036 2-0.724 0 μg (r=0.999 8). The average recovery were 97.14% (RSD 1.10%) and 97.64% (RSD 1.39%). There was a good linear relationship when the concentrations of sipeimine and sipeimine-3β-D-glucoside were within the range of 0.057 1-1.142 μg (r=0.999 1), 0.081 3-1.626 μg (r=0.999 5). The average recovery was 97.55% (RSD 1.75%) and 98.09% (RSD 1.80%). Conclusion: The method was accurate, sensitive, reproducible and may be used in the determination of pinocembrin, cardamonin, sipeimine and sipeimine-3β-D-glucoside in HZNHS.关键词：Houzao Niuhuangsan;pinocembrin;cardamonin;sipeimine;sipeimine-3β-D-glucoside;HPLC-ELSD
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To determine the quality of chemical compositions in the different extracts of Engelhardtia roxburghiana leaves. Method: Rutin, gallic acid and oleanolic acid were used as the control samples. Spectrophotometry was used to determine the content of total flavonoids, total polyphenol and total saponins in the 95% alcohol extract and water extract of the leaves of E. roxburghiana. Result: The 95% alcohol extract content of total flavonoids, total polyphenols and total saponins were 48.01%, 34.01% and 20.09% respectively. The content of water extract was 42.04%, 33.25% and 17.84% respectively. Conclusion: This method is simple, accurate and reliable, which could be used to determine the quality of the three major chemical compositions in E. roxburghiana.关键词：Engelhardtia roxburghiana;flavonoids;saponins;polyphenol
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the method for determining curdione, curcumol, germacrone, curzerene, furanodiene and β-elemene in Curcuma zedoary by RP-HPLC. Method: A Hypersil ODS column (4.6 mm×250 mm, 5 μm) was used with the mixture of acetonitrile-0.1% phosphoric acid as the mobile phase in gradient elution. The flow rate was 1.0 mL·min-1. The column temperature was kept at 25 ℃. The detection wavelength was set at 215 nm. Result: The calibration curves of curdione, curcumol, germacrone, curzerene, furanodiene and β-elemene were in good linearity over the ranges of 0.544-5.44 μg (r=0.999 6), 0.414-4.14 μg (r=0.999 8), 0.122-1.22 μg (r=0.999 5), 1.78-17.8 μg (r=0.999 3), 0.318-3.18 μg (r=0.999 4), 0.506-5.06 μg (r=0.999 5) respectively, and the average recoveries were 99.60% (RSD 2.71%), 101.48% (RSD 1.37%), 99.50% (RSD 2.47%), 100.29% (RSD 2.52%), 99.87% (RSD 1.51%), 100.58% (RSD 1.33%) respectively. Conclusion: The method is sensitive, accurate;the separation effect is good without interference. It can provide a basis as evaluation standard for quality of Rhizoma Zedoariae, and provide scientific basis for clinical rational drug use.关键词：HPLC;curdione;curcumol;germacrone;curzerene;furanodiene;β-elemene
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a new method to identify the peeled and unpeeled pieces and provide the basis of quality control for the pieces of Paeoniae Radix Alba by determining the content of paeoniflorin. Method: Two samples were identified by TLC and the content of paeoniflorin was compared by HPLC chromatography. Result: There had significant differences in two kinds of pieces. The unpeeled pieces had one extra spots more than the peeled pieces and the HPLC chromatography showed two extra peaks expect 12 common peaks. The content of paeoniflorin in Paeoniae Radix Alba decreased after peeled. Conclusion: The content of paeoniflorin in Paeoniae Radix Alba decreased after peeled and the established method was simple;it could be used for evaluation of different pieces of Paeoniae Radix Alba. Meanwhile, it also enriches the quality evalution method.关键词：Paeoniae Radix Alba;peeled pieces;unpeeded pieces;paeoniflorin;chromatographic identification;assaying
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the plasma concentration of orcinol glucoside of Curculiginis Rhizoma in blood on normal and deficiency cold state in rats, to explore that the correlation of Curculigini Rhizoma metabolize and and biological effects. Method: Male SD rats were randomly divided into two groups, namely the normal group and the deficiency cold group. The deficiency cold rats were given hydrocortisone sodium succinate injection(20 mg·kg-1·d-1) by intramuscular for 14 days. From the 15th day Curculigini Rhizoma(30 g·kg-1·d-1) water extracts was given ig to two groups rats for 7 days. After the last time, blood was collected for detecting plasma concentration of orcinol glucoside at multiple time points by HPLC, and calculate metabolic parameters. Result: The plasma concentration was increased in deficiency cold rats at 20 min, plasma concentration reached the maximum at 120 min, high level was maintained to 300 min;time to maximum plasma concentration(Tmax) and area under the curve(AUC) were increased significantly(P<0.05). Conclusion: The plasma concentration is high level in deficiency cold rats, that may provide the material basis for biological effect of Curculiginis Rhizoma.关键词：Curculiginis Rhizoma;clinic;different states;plasma concentration
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To analyze metabolites of puerarin in rats model with syndrome of dampness-heat in large intestine. Method: Rats model with syndrome of dampness-heat in large intestine was established,metabolites of puerarin in urine from model rats with syndrome of dampness-heat in large intestine were analyzed by UPLC-DAD-MS. Result: Eight components were identified as isomer of puerarin-O-glucuronide,puerarin-O-glucuronide,puerarin,daidzein-O-glucuronide,methyldaidzein-O-glucoside,isomer of methyldaidzein-O-glucuronide,methyldaidzein,methyldaidzein-O-glucuronide. Conclusion: Puerarin was excreted in the form of two-phase metabolites with glucuronide conjugate and glucoside conjugate,methylated metabolite as well as prototype in urine of model rats with syndrome of dampness-heat in large intestine.关键词：puerarin;syndrome of dampness-heat in large intestine;metabolites;UPLC;structural analysis
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To prepare oleanolic acid nanoliposomes and investigate its in vivo pharmacokinetics behavior in rats. Method: Oleanolic acid nanoliposomes were prepared by reverse-phase evaporation and characterized,entrapment efficiency was determined by sephadex column chromatography.Rats were injected with oleanolic acid nanoliposomes and oleanolic acid solution prepared by ourselves via the tail,respectively.The plasma concentrations of oleanolic acid from samples were determined by HPLC,with protopanaxadiol as internal standard.Pharmacokinetic parameters and non-compartment models were analyzed by t-test and PK-Slover 2.0 software,respectively. Result: Oleanolic acid nanoliposomes were round or oval vesicles with the mean diametre of (98.11±0.32) nm and encapsulation efficiency of (81.2±1.21)%.The plasma concentration-time curves of the nanoliposomes and solution conformed to non-compartmental model.By comparing with oleanolic acid solution,t1/2 and MRT of oleanolic acid nanoliposomes extended,AUC0-t increased and CL_obs decreased. Conclusion: Oleanolic acid nanoliposomes with high entrapment efficiency and small particle size had significantly improved bioavailability of oleanolic acid and prolonged its in vivo retention time.关键词：oleanolic acid;nanoliposomes;pharmacokinetics;particle size;encapsulation efficiency
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Erigerontis Herba and Paeoniae Radix Rubra compound with different proportion and two different routes of administration(intravenous and oral) on brain damage in the rat model of cerebral focal ischemia and reperfusion. Method: SD rats were divided into four groups randomly, normal control group,ischemia-reperfusion group,positive control group and different proportion of Erigerontis Herba and Paeoniae Radix Rubra compound group, 16 in each group. The Longa method was used to establish the MCAO model. Paeoniae Radix Rubra compound at different proportion was injected or given by gavage at different time points according to the test design. Based on factorial experiment design and factor analysis method, the whole combinations of each factors(route, proportion) and each levels(four different ratio of formula) were designed. The influence significance of each factors and their interaction for indexes were analyzed. Also,the significant difference of all the levels of every factor was observed. Result: The compatibility of Erigerontis Herba and Paeoniae Radix Rubra at a ratio of 1:1.5 was found, which could obviously improve pivotal biological indicator,and include neuroethology, the rate of cerebral infraction, and so on. Conclusion: The compatibility ratio of Erigerontis Herba and Paeoniae Radix Rubra of is 1:1.5, which exerts a protective effect on cerebral ischemia-reperfusion injury.关键词：Erigerontis Herba;Paeoniae Radix Rubra;MCAO;cerebral ischemia-reperfusion injury;Brain protection;factorial analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effects and mechanism of Fuzi Lizhong decoction(FZLZ) on immune cell factors in rats with spleen Yang deficiency syndrome. Method: One hundred Wistar rats were randomly divided into model group, FZLZ low-dosage group, middle-dosage group, high-dosage group of FZLZ and control group. Each group included 20 rats. Low-dosage group, middle-dosage group and high-dosage group were ig given with FZLZ according to 5,10, 20 g·kg-1based on model group, once a day for four weeks. Then thymus index, spleen index, the contents of interleukin(IL) -2, IL-6, IL-10 and tumor necrosis factor-α(TNF-α) were examined. Result: Compared with control group, thymus index, spleen index, the contents of IL-2, IL-6 and TNF-α in model group reduced (P<0.05 or P<0.01),however, the content of IL-10 increased significantly (P<0.01). Compared with model group, thymus index, spleen index, the contents of IL-2, IL-6 and TNF-α in both middle-dosage group and high-dosage group increased (P<0.05 or P<0.01)while the content of IL-10 reduced (P<0.05 or P<0.01). Conclusion: Fuzi Lizhong decoction can improve immune function of rats with spleen Yang deficiency syndrome by regulating the contents of IL-2, IL-6, IL-10 and TNF-α, which may be one of the mechanisms.关键词：Fuzi Lizhong decoction;spleen yang deficiency syndrome;immune;mechanism
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Lurong Jiangu dripping pills on the bone mineral density (BMD) of femur and expression of TRPV5 in kidney of rats with glucocorticoid-induced osteoporosis (GIO), explore the mechanism of GIO. Method: Eighty-two Wistar rats were randomly divided into normal group(A),model group(B),Lurong Jiangu dripping pills group(C) and Gushukang group(D). By intramuscular injection of dexamethasone(2.5 mg·kg-1)twice a week rat models was established. The treatment lasted for 9 weeks BMD of femur in vitro was detected with dual-energy X-ray absorptiometry, the mRNA and protein expression of TRPV5 in kidney were detected by real-time quantitative RT-PCR and Western blot methods. Result: Compared with the normal group, the BMD of model group rats decreased evidently(P<0.01), mRNA and protein expression of TRPV5 in kidney of model group rats decreased(P<0.01). Compared with the model group, the BMD of Lurong Jiangu dripping pills group increased(P<0.01), mRNA and protein expression of TRPV5 also increased(P<0.01). Conclusion: The depressant effect of dexamethasone for the calcium transport in kidney was the main pathomechanism of GIO.Lurong Jiangu dripping pills can upregulate the mRNA and protein expression of TRPV5 in kidney to promote renal calcium reabsorption.关键词：Lurong Jiangu dripping pills;GIO;BMD;TRPV5
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the influence of Hei Xiaoyao San on superoxide dismutase(SOD), gluthathione peroxidase(GSH-Px) and malondialdehyde(MDA) of brain tissue and serum in Alzheimer's disease rat model induced by β-amyloid protein(Aβ25-35) fragments so as to discuss the role and mechanisms involved in Hei Xiaoyao San in the prevention and treatment of Alzheimer's disease(AD). Method: The SD rats (n=94) were randomly divided into sham surgery rats(n=16) group and model group (n=94), and all rats of model group received water maze tests for 7 days. These eligible rats were randomly divided 5 groups, model group, positive control group, Hei Xiaoyao San high, medium and low dose groups,14 rats in each group. Aβ25-35fragments was injected into the hippocampus to damage brain neuron to make the rat's AD model. 7 days later, all rats of Hei Xiaoyao San group were orally administered Hei Xiaoyao San at low, medium and high doses (4.2, 8.50, 17.0 g·kg-1·d-1), the positive control group was orally given huperzine A solution 0.02 mg·kg-1·d, model group and sham group received an equal volume double distilled water daily for 28 d. After the last administration, the rats were sacrificed and serum and brain samples collected,and the serum and brain homogenate stration of SOD, MDA, GSH-Px were examined by means of chemistry chromatometry. Result: Compared with the sham group, serum and brain tissue homogenate SOD, GSH-Px of model group decreased significantly, MDA content increased significantly(P<0.05). Compared with the model group, Hei Xiaoyao San obviously increased the activities of SOD and GSH-Px and reduced the content of MDA (P<0.05) in serum and brain tissue. Conclusion: Hei Xiaoyao San can protect rat's AD model from damage, and the mechanisms of which may be related to anti-free radicals.关键词：Hei Xiaoyao San;Alzheimer’s disease;Aβ25-35 fragments
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to investigate the effects of Zhengan Xifeng decoction on the spontaneous movement in 1-methy-4-phenyl-1,2,3,6-tetrahy-dropyridine(MPTP)-treated C57BL/6 mice, and some mechanisms involved. Method: C57BL/6 mice were given MPTP (30 mg·kg-1) to prepare Parkinson disease mice model. Different doses of Zhengan Xifeng decoction (12.5, 25, 50 g·kg-1), or selegiline (10 mg·kg-1) were given for 14 d in the pretreatment groups. The behavioral analysis of C57BL/6 mice was performed by using spontaneous movement test. Real-time PCR and Western blot were used to analyze the expression of 14-3-3 and tyrosine hydroxylase (TH) mRNA and protein. Result: Zhengan Xifeng decoction could reduce the number of spontaneous movement of MPTP-treated C57BL/6 mice. Zhengan Xifeng decoction could also up-regulate the expression of 14-3-3 and TH mRNA and protein. Conclusion: Anti-Parkinson disease effect of Zhengan Xifeng decoction may be attributable to up-regulation mRNA and protein expression of 14-3-3 and TH in MPTP-treated C57BL/6 mice.关键词：parkinson disease;Zhengan Xifeng decoction;14-3-3;tyrosine hydroxylase
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Liuwei Buqi capsule(LBC) on the expression of STAT4 and STAT6 protein and to study the therapeutic effects and possible mechanism in rat model with Fei-qi deficiency syndrome(FQDS) of chronic obstructive pulmonary disease(COPD). Method: The rats were randomly divided into normal group, model group, high dose of Liuwei Buqi group (0.16 g·kg-1), low dose of Liuwei Buqi capsule group (0.04 g·kg-1), Jinshuibao capsule group(49.5 mg·kg-1), spleen aminopeptidase group(0.033 mg·kg-1),(n=12). The rat model of lung deficiency of COPD was established by smoking rats and dripping the LPS into the trachea. At twenty-eight days after the modeling. corresponding drugs were given.The expression of STAT4 and STAT6 protein of lung tissue was observed. Result: Compared with the normal control group, the average optical density of STAT4 protein in lung tissue and the positive area in model group increased markedly, STAT6 protein decreased (P<0.01).Compared with the model group, the positive area of STAT4 became smaller and the average optical density became weaker, the positive area of STAT6 increased;meanwhile, no statistical significance was found among the treatment groups. Conclusion: The LBC can inhibit the expression of STAT4 protein, and promote the expression of STAT6 protein.关键词：Liuwe Buqi capsule;chronic obstructive lung disease;Fei-qi deficiency syndrome;STAT4;STAT6
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of three different extracts from Malus toringoides (E Se)on experimental diabetic mice. Method: The mice were intraperitoneally injected with streptozocim(STZ,150 mg·kg-1) or alloxan(ALX),200 mg·kg-1) to establish diabetic mice. Then the diabetic mice were administrated with E Se flavonoid(180 mg·kg-1),E Se Jingao(900 mg·kg-1)and Kangzhu Ganlu(360 mg·kg-1) by intragastric in grouped mice respectively once daily for 4 days. All mice were fasted for 5 h before administration and the fasting blood glucose (FBG) was tested 1h after treatment. Body weight was determined by every other day. At the end of treatment, the mice were sacrificed in fasting condition after the FBG detection. The blood was collected for insulin and c-peptide detection. Once blood had been collected, the thymus and liver were immediately removed, individually weighed and expressed in relative terms. Result: Compared with the control group, the general behavior of mice treated with extracts from E Se became better and the FBG levels were significantly reduced(P<0.05). Significant increase in serum insulin (P<0.05) and decrease in c-peptide were detected in treated mice(P<0.05). The group of E Se Jingao and Kangzhu Ganlu showed a significant decrease in liver, relative masses compared with model group(P<0.05). Conclusion: The three different extracts from E Se all have a high hypoglycemic activity, further investigations are needed to explore the exact mechanism of action for antidiabetic effect.关键词：Malus toringoides;type 1 diabetes mellitus;Hypoglycemic effect
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the apoptosis-inducing effects of Weikang Shuning in human gastric cancer cells in vitro l. Method: The SGC-7901 cells were cultured in the Weikang Shuning liquid with the concentration is 200,400,800 mg·L-1for 48 and 72 hours,then the cells were collected and the flow cytometry was used to detect the change of mitochondria electric potential. ELISA method was used to detect the change of cytochrome(Cyt c). Prepare of cell slides, the immunocytochemistry method was used to study the effects of Weikang Shuning on the indexes of the induced SGC-7901 including Bcl-2 and Bax. Result: Weikang Shuning could decreased the membrane potential and release of Cyt c of gastric cancer cells (P<0.05). The expression of Bax was strengthened (P<0.05), and the expression of Bcl-2 was lessened (P<0.05). Conclusion: The mechanism of promoting gastric cancer apoptosis by Weikang Shuning in vitro may be associated with the mitochondrial apoptotic pathway.关键词：Weikang Shuning;gastric cancer apoptosis;mitochondrial apoptosis pathway;experimental study in vitro
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the influence of Qisang Jiangtang Fang (Q-S) on blood glucose,serum lipoprotein in type 2 diabetes(T2DM) rats. Method: T2DM model was induced with high sucrose-fat diet for 4 weeks and streptozocin (STZ)ip for 40 mg·kg-1,rats were randomly divided into groups:model group, Q-S low dose(ig 180 mg·kg-1·d-1)and middle dose(ig 360 mg·kg-1·d-1)and high dose group(ig 540 mg·kg-1·d-1),rosiglitazone treatment group(ig 4 mg·kg-1·d-1).Blood glucose,serum lipoprotein,hemoglobin Alc (HbAlc), insulin (Ins)contents in rats were detected after 60 d of continuous medication. Result: Compared with normal group, HbAlc levels were significantly increased (P<0.01), total cholesterol (TCH), total triglyceride (TG), low density lipoprotein (LDL) were significantly increased (P<0.05), high density lipoprotein (HDL)decreased significantly (P<0.05) in model group. Q-S can reduce the levels of blood glucose,HbAlc,TG,TCH and LDL,improve the level of HDL and Ins, those were significant deviation compare dwith the model group(P<0.05),it was no significant difference with the rosiglitazone treatment group. Conclusion: Q-S can effectively reduce rat's blood glucose of T2DM,promote Ins secretion, improve t the disorder of lipid metabolism in diabetic rats,regulate serum lipoprotein.关键词：Qisang Jiangtang Fang;type 2 diabetes mellitus;blood glucose;serum lipoprotein
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the scavenging activity in vitro of each part of ethanol extract of Rhamnella gilgitica Mansfeild et Melch. Method: R. gilgitica was extracted with 70% ethanol,then was extracted by petroleum ether, dichloromethane, ethyl acetate, n-butyl alcohol, ethanol the each part was configured to the appropriate solution,Prussian blue method Fenton method and 2,2-diphenyl-1-picrylhydrozyl(DPPH) were used to measure the antioxidantactivity. Result: The ethyl acetate, n-butyl alcohol and ethanol extract of R. gilgitica showed obvious scavenging effects, IC50 of the ethyl acetate, n-butyl alcohol and ethanol extract of R.gilgitica with DPPH was 0.060 5, 0.076 3, 0.083 3 g·L-1, respectively. Conclusion: The scavenging activity showed a dose-response relationship, even reach 60% by the above 1.0 g·L-1concentrations.The high polarity of solvents layer extract has the strongest free radical scavenging activity.关键词：Rhamnella gilgitica;Prussian blue method;Fenton method;DPPH;antioxidant activity
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of Buzhong Yiqi decoction on rats' wound healing, and to achieve the purpose of wound healing from medical treatment of spleen. Method: Sixty SD rats were randomly divided into normal group, spleen deficiency model group and Buzhong Yiqi decoction group (7 g·kg-1), and each group is divided into two subgroups of postoperative 3 d and 7 d. Model group and Buzhong Yiqi decoction group were fed with Rhubarb (20 g·kg-1) for 18 days, and normal group was given an equal volume of distilled water. From the 19th day, rats of each group were made the surgical wound;Buzhong Yiqi decoction group were administered with Buzhong Yiqi decoction until the date of sacrifice, and other groups given an equal volume of distilled water. After rats were sacrificed, muscle tissue of wound was prepared. Hydroxyproline (Hyp) was measured using alkaline hydrolysis, and vascular endothelial growth factor (VEGF) and α-smooth muscle actin (α-SMA) were detected using Western blot. Result: Compared with the normal group, Hyp, VEGF and α-SMA of model group were significantly decreased (all P<0.01). Compared with model group of postoperative 3 d, Hyp, VEGF and α-SMA of Buzhong Yiqi decoction group of postoperative 3 d were significantly increased (P<0.01, P<0.01, P<0.05), but much lower than those of normal group of postoperative 3 d (all P<0.05). Compared with model group of postoperative 7 d, Hyp, VEGF and α-SMA of Buzhong Yiqi decoction group of postoperative 7 d were significantly increased (P<0.01, P<0.05, P<0.01), but there were no statistical difference between Buzhong Yiqi decoction group and normal group. Conclusion: Buzhong Yiqi decoction can effectively promote wound healing by increasing Hyp, VEGF and α-SMA, and extension of administration time is more conducive to wound healing.关键词：Buzhong Yiqi Decoction;spleen deficiency;hydroxyproline;α-smooth muscle actin;vascular endothelial growth factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Safflower injection on gene transcription and protein expression of B cell lymphoma/leukemia-2(Bcl-2) and Survivin in HepG-2 cell so as to explore its mechanism of inducing apoptosis in human hepatma cell line HepG-2. Method: HepG-2 cells were treated in vitro with different concentrations(0,50,100,150,200,250 g·L-1) of Safflower injection. Inhibiting effect of cell proliferation was measured by methylthiazolyl tetrazolium (MTT) assay. Cell apoptosis rates were analyzed by flow cytometry (FCM).Quantitative real-time RT-PCR and Western blot methods were used to detect the mRNA and protein expressions of apoptosis related factors Bcl-2 and Survivin. Result: The results indicated that Safflower injection could inhibit the proliferation of HepG-2 cells in a dose and time dependent manner(P<0.01).As the concentration of Safflower injection increased, the apoptosis rates of the cells were increased. Both the mRNA and protein expressions of Bcl-2 and survivin were down-regulated. Conclusion: Safflower injection could inhibit the proliferation and induce cell apoptosis of human hepatoma cell line HepG-2.The down-regulating expressions of Bcl-2 and Survivin on both mRNA and protein levels maybe one of the main mechanisms for Safflower injection inducing apoptosis of HepG-2 cells.关键词：Safflower injection;human hepatoma cell line HepG-2;cell proliferation;cell apoptosis;Survivin;Bcl-2
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effects and mechanisms of isoflovone(IS) on spatial learning, memory and monoamine neuro-transmitte, gonadotropin-releasing hormone(GnRH) inovariectomized rats. Method: The fifty Sprague-Dawley rats were ovariectomized. Ovariectomized rats were randomly divided into five groups, model group, estradiol benzoate(EB) group, IS low dose group, IS middle dose group, IS high dose group. EB group were treated with estradiol benzoate(0.2 mg·kg-1) by intraperitoneal injection once a week. IS groups were treated with IS(30,60,120 mg·g-1·d-1) by gavage one time daily. The spatial learning and memory performance were evaluated with Morris watermaze task. The contents of monoamine neuro-transmitter in hippocampus were measured, and GnRH in hypothalamus was measured. Result: ① As compared with control group, the latency period of model group increased significantly(P<0.05).Compared with model group, the latency period of EB group and IS middle dose group decreased significantly(P<0.05).②As compared with control group contents of norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in hippocampus of model group decreased significantly(P<0.05). As compared with model group, contents of NE in hippocampus of IS high dose group increased significantly(P<0.05).As compared with model group, contents of DA and 5-HT in hippocampus of IS middle dose group, EB group increased significantly(P<0.05). ③As compared with control group, contents of GnRH in hypothalamus of model group increased significantly(P<0.05).As compared with model group, contents of GnRH in hypothalamus of IS middle and high dose group,EB group decreased significantly(P<0.05). Conclusion: Isoflovone can improve the ovariectomized rats' impairment of spatial learning and memory by increasing contents of NE,DA,5-HT in hippocampus. Otherwise, isoflovone can decrease contents of GnRH in hypothalamus.关键词：isoflovone;learning and memory;monoamine neuro-transmitter;GnRH
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: A comparative study of two boiling method (ephedra first boiling and four herbs with boiling) the proceeds Maxin Shigan decoction of influenza A virus infection in mice. Method: Nasal inoculation method to establish the type A influenza virus lung infection in mice model, experimental set normal comparison group, model comparison group, ephedra first boiling group, four herbs with boiling group, oseltamivir group, 7 days of continuous given medicines by intragastric. Respectively, 4 days and 7 days after infection to detect the body weigh of mice, on the 7th day, routine testing lung weight index;observation of lungchanges by optical microscopy techniques, lung tissues were observed ultrastructure by transmission electron microscopy techniques. Result: ① Body weight:4 days and 7 days after infection,compared with the normalcomparison group, model comparison group significant weight loss(P<0.05), 7 days after therapy, compared with the model comparison group,Oseltamivir group, ephedra first boilinggroup significant weight gain(P<0.01 or P<0.05).② Lung index:compared with the normal comparison group,the mouse lung index of model comparison group increased significantly(P<0.01).Compared with the model comparison group,lung index was significantly reduced in all treatment groups(P<0.01 or P<0.05). The significant difference(P<0.01)about of the ephedra first boiling group compared with the model comparison group is higher than thefour herbs with boiling group compared with the model comparison group on the significance(P<0.05).③Lung pathological changes:compared with the normal comparison group,the mouse lungs normal tissue structure disappeared,the alveolar inflammatory cell infiltration. Compared with the model comparison group,lung tissue of all treatment groups pathological change significantly improvely,among oseltamivir group and ephedra first boiling group,the level of lung disease in mice is lighter than four herbs with boiling group.④ Lung tissue ultrastructure:compared with thenormal comparison group, the mouse lung tissue ultrastructure of model comparison group was damaged,theperformance of the nuclear membrane and the plasma membrane are blurred,reduced organelle, the mitochondrial cristaeis reduce ordisappear,endoplasmic reticulum emerges vacuolar lesions,there are a large number of virus particles in the cytoplasm, each treatment group lung tissue structure has been improved.Among oseltamivir group and ephedra first boiling group,the extent of lung tissue ultrastructure diseasein mice is completer than four herbs with boiling group. Conclusion: The ephedra first boiling decoction is more effective than the four herbs with boilingdecoction on againning type A influenza virus.关键词：Maxing Shigan decoction;boiling method;type A influenza virus;lung index;pathological changes;ultrastructure
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the potent active components of Cimicifugae Rhizoma for treating hepatitis B virus(HBV). Method: The total phenolic acid and saponins of Cimicifugae Rhizoma were separated by macroporous resin, HBV DNA, HBsAg and HBeAg were observed to analyze the anti-hepatitis B virus effect of prim-o-glucosylcimifugin, ferulic acid, Cimicifugae Rhizoma aqueous extract solution, Cimicifugae Rhizoma phenolic acid and Cimicifugae Rhizoma saponins. Result: The five groups significantly reduced the concentration of HBsAg and HBeAg. Compared with the blank control group, the total phenolic acid can decrease the HBsAg and HBeAg (P<0.01) and the ferulic acid was the potential active component, Cimicifugae Rhizoma saponins can reduce the HBsAg (P<0.05) but prim-o-glucosylcimifugin have no obvious anti-hepatitis B virus effect. The total phenolic acid of Cimicifugae Rhizoma can remarkably decrease the level of viral core associated-HBV DNA in the cytoplasm. Conclusion: The total phenolic acid of Cimicifugae Rhizoma is the active part to anti-hepatitis B virus effect, and the results provide the basis of screening new drugs for treatment hepatitis B virus.关键词：Cimicifugae Rhizoma;hepatitis B virus;phenolic acid;saponin;prim-O-glucosylcimifugin;ferulic acid
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Observe the effects of Jiedu Quyu prescription combined with chemotherapy on expression of Bcl-2/Bax and apoptosis of MCF-7 cells. Method: Use the MTT to determinate the inhibitory action of medicine to MCF-7 cells; flow cytometry to detect the apoptotic index; immunocytochemistry to examine the Bcl-2, Bax expression. Result: Jiedu Quyu prescription can inhibit the MCF-7 cells proliferation, and on time, drug concentration-dependent; of chemotherapy synergies. Flow cytometry find that Jiedu Quyu prescription on inducing apoptosis in MCF-7 cells early advantage; and it also can decreasing the Bcl-2 expression in MCF-7 cells, also depend on time and drug concentration. Conclusion: The Jiedu Quyu prescription displayed its effect of inhibiting and inducing to apoptotic. It may be associated with the down-regulate of the expression of Bcl-2.关键词：breast cancer;Jiedu Quyu prescription;MCF-7;flow cytometry;bcl-2/bax
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the influence of Euphorbiae Humifusae herb (EHH) on anti-tumor effect and the expression of apoptosis protein in transplanted liver cancer mice. Method: H22 liver cancer cells were inculated in the right axilla to establish solid tumor models. The animal models were randomized into five groups, the model control group, cyclophosphamide (1 mg·kg-1)group, the EHH(66, 132, 264 mg·kg-1)group. The medication was given continuously for 14 days. The tumors were weighted. SOD activity and MDA content of serum were measured by the colorimetric analysis. The expression of Bcl-2, Bax and Caspase-3 in tumor tissues were determined by immunohistochemistry. Result: The size of tumor in EHH treat groups was significantly smaller than that in model control group(P<0.05). EHH could significantly decrease MDA level and increase SOD level(P<0.05). The expressions of Bcl-2 were decreased, while the expressions of Bax and Caspase-3 were increased in EHH high,middle dore groups(P<0.05). Conclusion: EHH can inhibit the growth of liver cancer cell and its mechanism is probably related to increased antioxidation, decreased Bcl-2 expression and increased Bax and Caspase-3 expression in transplanted liver cancer mice.关键词：herba Euphorbiae Humifusae;antioxidation;Bcl-2;Bax;Caspase-3
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Xiaobanxia decoction on the movement of isolated ileum in guinea pig and analyze the mechanism of anti-emetic effect of Xiaobanxia decoction(XBXD). Method: The effects of XBXD(0, 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 20, 40 g·L-1) on ileum spontaneous contraction tension, amplitude and frequency were investigated in organ bath. Emesis-relating agonists acetylcholine (Ach), histamine (His), 5-hydroxytryptamine (5-HT), dopamine (DA) were used as tool drugs, the inhibitory effect of XBXD (0, 2, 5, 10 g·L-1) on contraction tension, amplitude and frequency of isolated ileum in guinea pig were observed. Result: XBXD could dose-dependently inhibit ileum spontaneous contractions, reducing the average tension, amplitude, frequency (P<0.05 or P<0.01 vs control). Ach, His and 5-HT could stimulate ileum contraction, enhance the tension, amplitude and frequency. All the three doses (10, 5, 2 g·L-1) of XBXD could suppress the ileum contraction stimulated by above three tool drugs, significantly reduced the contraction tension, amplitude and frequency (P<0.05 or P<0.01 vs before XBXD or XBXD 0 g·L-1). DA could inhibit the spontaneous contraction, the middle and small dose (5, 2 g·L-1) of XBXD could antagonize the inhibitory effect of DA, and enhance the contraction tension, amplitude and frequency (P<0.05 or P<0.01 vs before XBXD or XBXD 0 g·L-1). Conclusion: XBXD can inhibit spontaneous contractile activity of ileum in guinea pig, the mechanism may be related to blocking M, H1, 5-HT and D2receptors or directly suppressing ileum smooth muscle. The mechanism of anti-emetic effect of XBXD needs further study.关键词：Xiaobanxia decoction;isolated ileum;M Receptor;H1 receptor;5-HT receptor;D2 receptor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the neuroprotection effect of Zhongfeng 1 on cerebral infarction caused by acute cerebral ischemia in mice and focal cerebral ischemia in rats. Method: Acute cerebral ischemia model in mice was induced by ligaturing the bilateral carotid arteries and vagus nerve, and breathing duration was observed.Cerebral ischemia and infarction in rats were induced by middle cerebral artery occlusion (MCAO). The effects of Zhongfeng 1 were evaluated in the two models. The different doses of Zhongfeng 1 were administrated before modeling (31.74,15.87,7.94 g·kg-1 and 24.78, 12.39,6.20 g·kg-1 respectively for mice and rats). In mice, Zhongfeng 1 was given for 8 days before modeling. In rats, Zhongfeng 1 was given for 3 days before modeling. Nimodipine was used as the positive control. Result: Each dose group of Zhongfeng 1 could prolong the breathing duration in mice with acute cerebral ischemia, and the effect was dose-dependent, but without significant difference among different dose groups. Both Zhongfeng 1 high dose group and middle dose group, compared with the model control group, could significantly reduce the proportion of cerebral focal infarction(P<0.001, P<0.01) and significantly improve symptoms of nerve function injury in rats. Conclusion: Zhongfeng 1 has good pharmacological effects on cerebral ischemia in mice and rats. It can prolong the maintenance of mice breathing, significantly reduce infarction ratio of cerebral ischemia, and improve the symptoms of nerve damage.关键词：Zhongfeng 1;cerebral ischemia;stroke;animal model
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect of the total flavonoids of Bidens bipinnata (TFB) on the TGF-β1/smad pathway of liver tissue in rats with liver fibrosis. Method: Liver fibrosis in rats was established by CCl4, and then the rats were randomly divided into five groups:model group, colchicine group (0.2 mg·kg-1), low-, medium-and high-dosage groups of TFB (60, 120, 240 mg·kg-1), as well as the normal group was set up. The drugs were given to rats daily for 30 consecutive days. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBil) in serum were examined, and the levels of hyaluronic acid (HA), laminin (LN), precollagen III (PCIII) and hydroxyproline (Hyp) in hepatic tissue were examined. The transforming growth factor-β1(TGF-β1)-immunoreactive cells were determined by immunohistochemical assay, and the expression of smad2 and smad3 in hepatic tissue was determined by Western blot. In addition, the hepatic pathological changes were observed by Masson staining. Result: Compared with model group, TFB effectively decreased the activities of AST, ALT and TBil in rats with liver fibrosis (P<0.01), the contents of HA, LN, PCIII and Hyp in homogenate were decreased (P<0.01). The quantity of TGF-β1-immunoreactive cells was reduced (P<0.01). The smad2 and smad3 protein level was down-regulated in hepatic tissue (P<0.01). Moreover, liver fibrosis was alleviated. Conclusion: TFB has protective effect on the CCl4-induced liver fibrosis in rats, the mechanism may be implicated in regulating the TGF-β1/smad pathway to inhibit collagenation.关键词：Bidens bipinnata;total flavonoids;liver fibrosis;TGF-β1/smad pathway;collagen
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the long-term effect of Rhubarb on the uterus and ovary in female rats. Method: Ninety 8-week-old female rats (180-220 g) were randomly divided into nine groups:normal control group, and investigation groups (n=10 each, administration of Rhubarb 1.0,2.0 g·kg-1 for 30, 40, 50, 60 d respectively).Then the index of uterus and ovary were calculated, and the morphological changes in uterus and ovary were observed. Result: The indexes of uterus and ovary in Rhubarb 1.0 g·kg-160 d group were lower than that in the control group obviously (P<0.05);the indexes of uterus in 2.0 g·kg-1 groups (40, 50, 60 d) were lower than that in the control group obviously (P<0.05 or P<0.01). While, the indexes of ovary in 2.0 g·kg-1 groups (30, 40, 50, 60 d) were lower than that in the control group obviously (P<0.05 or P<0.01). The pathological examination showed that endometrial edema and glandular reduction, ovary atrophy, follicles reduction, and follicle cells irregular arrangment in 2.0 g·kg-1 groups (50, 60 d). Conclusion: The long-term use of Rhubarb shows a certain degree of reproductive toxicity in female rats, and its impact is clear dose/time dependent.关键词：Rhubarb aqueous extract;Uterus;ovary
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the analgesic effect of Wumei pill and its disassembled prescriptions on ulcerative colitis (UC) in rats. Method: Experimental animals were divided into control group, model group, Wumei pill group, cold and heat group, heat group, cold group, tonic group, convergence group. According to the decomposition principle, Each group was respectively given water decoction of Wumei pill(13.3 g·kg-1),cold and heat prescription(9.0 g·kg-1), heat prescription (5.33 g·kg-1), cold prescription (3.67 g·kg-1), convergence prescription(2.67 g·kg-1), and tonic prescription(1.67 g·kg-1) by gavage. The control group and model group were given with saline(10 mL·kg-1). Analgesic effect of Wumei pill and its prescriptions were observed by hot plate and writhing. Ulcerative colitis model was established with trinitro-benzene-sulfonic acid(TNBS)/ethanol. The PGE2 content of colonic mucosa was determined by enzyme-linked immunosorbent assay. Result: Compared with blank group, the Wumei pill group, cold and heat group and heat group could prolong the pain threshold of mice, reduce the writhing times and decrease the PGE2 content of colonic mucosa in rats(P<0.05). Tonic group had partly analgesic effect but the effect was weaker than Wumei pill group(P<0.05). Conclusion: Wumei pill has obvious analgesic effect, while tonic group has partly analgesic effect. The drug of heat group play an very important role for analgesic effect.关键词：Wumei pill;disassembled prescriptions;analgesia;PGE2;hot plate;writhing
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effects of Tussah male moths oil on the content of testosterone, substance metabolism and anti-fatigue ability of rats. Method: By using the model of high-intensity endurance training, Wistar rats were divided into 5 groups, 10 in each group control group (C group), motion control group (T group), exercise+ig low-dose Tussah male moths oil group (TML group), exercise+ig middle-dose Tussah male moths oil group (TMM group), and exercise+ig high-dose Tussah male moths oil group (TMH group). Administration was performed once a day. The rats in Tussah male moths oil groups were gavaged with 0.5,1, 3 g·kg-1 with ig volume of 5 mL·kg-1. The rats in C and T groups were given same volume saline. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: The body weight of the rats in Tussah male moths oil groups was increased compared with T group (P<0.05), and the swimming time was longer than T group (P<0.01). Serum corticosterone was lower than the T group (P<0.01). Changes in the ratio of serum testosterone/corticosteron were more consistent with testosterone changes among the groups. Liver glycogen (P<0.05), muscle glycogen (P<0.01) and hemoglobin(P<0.01) in Tussah male moths oil group were higher than in T group, but the serum urea nitrogen was lower than the T group (P<0.01). Conclusion: Tussah male moths oil can alleviate the impact of high-intensity exercise on serum testosterone, and maintaine it at normal physiological levels;it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.关键词：Tussah male moths oil;testosterone;anti-fatigue
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the stimulation effect of 19 traditional Chinese medicine injections(TCMIs)on rat peritoneal mast cell degranulation in vitro. Method: The optimal reaction factors were found to detect histamine of release caused by TCMIs. And rat peritoneal mast cells were isolated and purificated as reference described. The cells were then incubated in Tyrode's containing 100 mL·L-1 TCMI or 4 mg·L-1 C48/80 for 1 h. After washing, degranulation was assessed by measuring histamine in cell lysates as previously described,and percentage of degranulated peritoneal mast cell were calculated in a modified method. Result: The optimal reaction time of histamine and O-phthaldialdehyde(OPT) to form a fluorophore is 3 min.The fluorophore is stability during 7.5-30 min after the reaction was terminated. Percentages of histamine release in two calculation methods showed no difference. The mast cells, stimulated by Tan-re-qing, Qing-kai-ling, Shen-mai, Dan-shen, Xue-sai-tong, Shen-fu Shuang-huang-lian, Sheng-mai, Xiang-dan, Re-du-ning, Fu-fang-dang-gui,had higher percentages in histamine release than that in control. But, those stimulated by Mai-luo-ning,Huang-qi,Ai-di,Xing-nao-jing, Huang-qi-duo-tang,Hong-hua,Yan-suan-chuang-xiong-qin,Shen-qi had no difference with the control in degranulation. Conclusion: Most hypersusceptibility caused by TCMIs may be anaphylactoid reactions. It suggests that rat peritoneal mast cell degranulation test by detecting histamine release can be used to evaluate anaphylactoid reactions caused by TCMIs.关键词：traditional Chinese medicine injection;mast cells;degranulation;anaphylactoid reaction
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The purpose is to study the clinical curative effect of Liuwei Wuling tablet in the treatment of liver fibrosis in hepatitis B and its influences on the determination of monoamine oxidase (MAO) and transforming growth factor β1(TGF-β1). Method: Eighty-four cases of patients were randomly divided into observation group (42 cases) and control group (42 cases). Patients in both groups all took the conventional treatment (eg, vitamins, liver-protect drugs) and the entecavir by oral, 0.5 mg/time, 1 time/day. The patients in observation group took Liuwei Wuling tablet, 1.5 g/time, 3 times/day. The treatment course of two groups was 24 weeks. The liver function, liver fibrosis indexes, MAO and TGF-β1 were tested before and after the treatment, examining liver and spleen by ultrasound B. Result: After the treatment, the total effective rate of observation group was 90.5%, Better than the control group 61.9% in the control group(P<0.01). The level of Alanine aminotransferase(ALT), aspartate transaminase(AST) and total bilirubin(TBIL) of observation group was lower than those of control group (P<0.01). Also, the serum hyaluronic acid(HA), laminin(LN), procollagen type Ⅲ(PCⅢ) and collagen type Ⅳ(Ⅳ-C) level of observation group were lower than the control group (P<0.01). The serum MAO and TGF-β1 level of the two groups were obviously decreased and the decline in observed group was more significantly (P<0.01). The spleen thickness of patients in observation group was lower than the control group after treatment (P<0.05). Conclusion: Liuwei Wuling tablet can inhibit the expression of serum MAO and TGF-β1 of the hepatitis B liver fibrosis patients, further to inhibit the activation of hepatic stellate cells, preventing the development of liver fibrosis. The clinical curative effect of Liuwei Wuling tablet is significant and worthy of promotion.关键词：chronic hepatitis B;liver fibrosis;Liuwei Wuling tablet;monoamine oxidase;transforming growth factor-β1
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The purpose is to observe the curative effect of Tongfu Qingyi recipe in in adjuvant therapy of severe acute pancreatitis (SAP)and its protection function to intestinal barrier function. Method: Sixty-four cases of SAP patients were randomly divided into control group (32 cases) and observation group (32 cases) according to the digital method. Patients in both groups were given conventional western medicine treatment. On the basis of the control group, patients in observation group took Tongfu Qingyi recipe, stomach filling, 1 dose/day, treatment course 10 days.Levels of serum D-lactic acid, diamine oxidase (DAO) and levels of plasma endotoxin and urinary lactulose/mannitol (L/M) values were monitored. The first defecation time, the abdominal pain relief time, the abdominal distension relief time and borborygmus recovery time were recorded. Levels of serum interleukin 6 (IL-6), interleukin 8 (IL-8) and tumor necrosis factor-α(TNF-α)were detected before and after treatment. Levels of plasma thromboxane B2 (TXB2) and 6-ketone-prostaglandin F1a (6-keto-PGF1a) were detected before and after treatment. Occurrence rate, transit operation rate and mortality rate of acute respiratory distress syndrome (ARDS) and multiple organ dysfunction syndrome (MODS) were recorded within 4 weeks. Result: On the fifth and tenth day after the treatment, levels of serum D-lactic acid, DAO and endotoxin and urinary L/M value were significantly decreased and were lower than the control group (P<0.01). The plasma endotoxin level in both groups showed a increased trend and observation group was lower than control group (P<0.01). The first defecation time, the abdominal pain and distension relief relief time and borborygmus recovery time of observer group were all shorter than control group (P<0.01). After the treatment, levels of serum interleukin 6 (IL-6), interleukin 8 (IL-8) and tumor necrosis factor-α(TNF-α) of observer group were lower than the control group (P<0.01) TXB2 level of observer group was lower than the control group, while 6-keto - PGF1a level was higher than the control group (P<0.01). Within the 4 weeks, the incidences of ARDS and MODS were 12.5% and 9.38%, which were lower than 37.5% and 31.25% of the control group (P<0.05). Conclusion: In the adjuvant therapy of SAP patients, Tongfu Qingyi recipe can alleviate intestinal paralysis and inflammatory injury, promote gastrointestinal function recovery and blood circulation. It protects the Intestinal barrier function of SAP patients and reduces the intestinal bacterial and endotoxin translocation, which further reduces the incidence of ARDS and MODS.关键词：severe pancreatitis;Tongfu Qingyi recipe;intestinal barrier function;inflammatory factor;microcirculation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy and mechanism of action of Yiqi Jiedu Ruangan decoction on patients with post-hepatitis B cirrhosis(PHBC)(compensated stage). Method: Seventy-six patients were randomly divided into observation group(n=38) and control group (n=38). Both groups were adopted conventional therapy such as liver protection, antiviral therapy. Control group was given Fuzheng Huayu capsule, 1.5 g each time, 3 times each day. Observation group were given Yiqi Jiedu Ruangan decoction, 1dose each day, conventional decocting with water for 2 times, all for 24 weeks. Liver function such as alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bilirubin(TBIL), albumin(ALB), the ratio of albumin and globulin(A/G) in pretherapy and post-treatment were tested, and indicators of hepatic fibrosis such as serum hyaluronic acid(HA), laminin(LN), serum procollagen type III(PCIII), serum collagen type IV(IV-C) were detected. The thickness of the spleen and the diameters of the portal vein (DPV), the diameters diameter of splenic vein(DSV), the portal vein velocity (VPV), the splenic venous blood flow (VSV) were determined by color Doppler ultrasound. The level of serum tissue inhibitor of metalloproteinase 1(TIMP-1), transforming growth factor-β1(TGF-β1),platelet-derived growth factor (PDGF) in pretherapy and post-treatment were tested, and indicators of hepatic fibrosis such as HA, LN, PCIII, IV-C were detected. The thickness of the spleen and DPV, DSV, VPV, VSV were determined by color Doppler ultrasound. the level of TIMP-1, TGF-β1, PDGF in pretherapy and post-treatment were tested. Result: The total effective rate of observation group(81.57%) were superior to control group(60.52%)(P<0.01);the level of ALT, AST, TBIL of observation group were descended than before and lower than control group(P<0.01);the level of ALB, A/G of both groups were risen and observation group were higher than control group(P<0.01 or P<0.05);the level of HA, LN, PCIII, IV-C of observation group were lower than control group(P<0.01);the improvement of the thickness of the spleen and DPV, DSV, VPV, VSV of observation group were superior to control group(P<0.01 or P<0.05);the level of TIMP-1, TGF-β1, PDGF of observation group were dramatic declined and lower than control group(P<0.01). Conclusion: Yiqi Jiedu Ruangan decoction can reduce the enzyme, protect liver function, anti-fibrosis and resist cirrhosis, and the mechanism of action may be related with reducing the level of TIMP-1, TGF-β1, PDGF. It is worthy of clinical use.关键词：chronic hepatitis B(CHB);cirrhosis;Yiqi Jiedu Ruangan decoction;tissue inhibitor of metalloproteinase 1;transforming growth factor-β1;platelet-derived growth factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The purpose is to study the curative effect of Tiaochong Pinggan square on hypertension of patients in perimenopausal period and its influence on vascular endothelial function and neuroendocrine function. Method: Ninety cases of hypertension patients in perimenopausal period were randomly divided into control group (45 cases) and observation group (45 cases) according to digital method. The patients in both groups took psychological supportive treatment. Patients in control group took the oryzanol tablets (20 mg/time, 3 times/day) and felodipine zyban tablets (5 mg/time, 1 time/day). Patients in observation group took Tiaochong Pinggan square, 1 dose/day, 2 times/day, conventional water frying. Treatment course was 8 weeks. Kupperman index (KI) was used to record symptoms of perimenopausal syndrome, detecting blood pressure levels of patients. Levels of serum estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), nitric oxide (NO), endothelin 1 (ET-1), neuropeptide Y (NPY), neural antihypertensive peptides (NT) and β-endorphin (β-EP) were measured. Result: After treatment, in fourth week, levels of SBP and DBP in both groups reduced, compared to the state before treatment (P<0.01). In eighth week, levels of SBP and DBP in both groups fall further, observation group was lower than control group (P<0.01). The rate of blood pressure recover was 68.89%, superior to 46.67% in the control group (P<0.05). The effect of blood pressure efficacy of observation group was 93.33%, better than 73.33% of control group (P<0.05). In 4 weeks after treatment and the end of the treatment, KI score of the observation group was lower than the control group (P<0.01), while the descend range of KI score in observation group was superior to the control group (P<0.01). After treatment, E2 level of observation group was higher than the control (P<0.05), but levels of FSH and LH were lower than the control group (P<0.01). ET-1 level of observation group declined and was lower than the control group (P<0.01). However, NO level of observation group increased and was higher than the control group (P<0.01). NPY of observation group was lower than the control group after treatment, while levels of NT and β-EP were higher than the control group (P<0.05). Conclusion: On the basis of psychological guidance treatment, Tiaochong Pinggan square can improve clinical symptoms of patients with perimenopausal syndrome, decreasing blood pressure level of patients and promoting the patients' blood pressure return to normal state. Its mechanism may be related to its regulation of vascular endothelial function and neuroendocrine function, which is worth of clinical use.关键词：perimenopausal syndrome;hypertension;Tiaochong Pinggan square;vascular endothelial function;neuroendocrine
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The purpose is to observe the clinical curative effect of this method in prevent recurrence of peptic ulcer (PU). Method: Ninety-six cases of PU patients were randomly divided into observation group (48 cases) and control group (48 cases). The patients in the control group took omeprazole enteric capsules by oral, 20 mg/time, 2 times/day. If the HP of patients is positive, they took conventional trigeminy anti HP treatment. The patients in the observation group took, 1 dose/day. After the treatment, the patients in control group took omeprazole enteric capsules, 20 mg/time, 1 time/day, treatment course 4 weeks. While the observation group taking the Yuyang Xiaotong prescription during the 4 weeks. Gastroscope inspection was operated before and after the treatment to evaluate endoscopic mucosal maturity and histological regenerative mucosal maturity. By using immunohistochemical method to detect the express of ulcer mucous membrane spasmolysis peptide (SP) to observe the changes of clinical symptoms, taking 1 year follow-up visiting to observe the recurrence situations. Result: The total effective rate of gastroscope inspection of the observation group was 97.9%, higher than 85.4% of control group (P<0.05). The evolutions of endoscopic mucosal maturity and histological regenerative mucosal maturity in the observation group were superior to control group (P<0.01). The total effective rate of syndromes curative effect in the observation group was superior to that in the control group, with the rate 100% and 79.1% (P<0.01). After the treatment, the symptom score of stomach pain, bloating, eating less and stay, loose stool, belching, pantothenic acid and other symptoms was lower than the control group (P<0.01). The SP positive expression in the observation group was obviously increased (P<0.01). The recurrence rate of observation group was 26.8%, lower than 60.0% of the control group (P<0.01). Conclusion: This method can significantly reduce the clinical symptoms and increase the healing quality of PU, decreasing the recurrence rate. The curative effect in short term and long term was obvious. The action mechanism may be related to the increase of SP expression.关键词：peptic ulcer;Yuyang Xiaotong prescription;the quality of ulcer healing;recurrence;ulcer mucous membrane spasmolysis polypeptide
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the influences of Buyi Qiangxin tablet on cardiac function and life quality of the chronic heart failure (CHF) patients. Method: Sixty-eight cases of patients with CHF were randomly divided into control group (34 cases) and observation group (34 cases)according to before and after entering the hospital. The patients of both groups took general treatment of cardiac failure and conventional western medical treatment according to the guide of the diagnosis and treatment of chronic heart failure. The patients in observation group took Buyi Qiangxin tablets, 1.2 g/time, 3 times/day, the treatment course is 4 weeks. The New York Heart Association (NYHA) cardiac heart grading and 6 minutes walk test (6 MWT) were evaluated before and after treatment, recording Lee's heart failure. Left ventricular ejection fraction (LVEF) and cardiac output (CO) were tested using echocardiography. The life quality was evaluated by Minnesota life quality of heart failure questionnaires. detecting the plasma NT-proBNP and antidiuretic hormone (ADH) level. Result: The total curative effect of Lee's heart failure of the observation group was 91.17%, which is higher than that of the control group (73.52%, P<0.05). The total curative effect of cardiac function of observation was 88.23%, superior to 67.64% in the control group (P<0.05). After the treatment, the Lee's heart failure points and life quality scores of observation group decreased and were lower than the control group (P<0.01). The 6MWT of both groups increased and the increasing in observation group was more significantly (P<0.01). The LVEF and CO of both groups increased after the treatment, the observation group was higher than control group (P<0.01). The levels of plasma NT-proBNP and ADH decreased and observation group was less than the control group (P<0.01). Conclusion: Buyi Qiangxin tablets can improve cardiac function grading of CHF patients, increasing LVEF, CO, 6 MWT and life quality of patients. Its mechanism may be related to decreasing NT-proBNP and ADH of patients.关键词：chronic heart failure;Buyi Qiangxin tablet;cardiac function;life quality
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of harnal capsule combined with Shaofu Zhuyu soup in treating chronic abacterial prostatitis/chronic pelvic pain syndrome. Method: One hundred and thirty patients of CPPS from September 2008 to October 2012 were randomly divided into A and B group, 65 cases in each group. A group received the treatment of harnal capsule, the B group received the treatment of harnal capsule combined with Shaofuzhuyu soup. Observe and compared the clinical effect of the both groups. The evaluating indexes included the change of the scores of NIH-CPSI, leukocyte count and lecithin corpuscles in prostatic fluid, peak flow. Result: The total effective rate in B group was higher than A group, and there was significant difference in both groups (P<0.05). The scores of NIH-CPSI, leukocyte count in B group were significantly lower than those in A group after treatment, and there was significant difference in both groups (P<0.05). The cases with lecithin corpuscles (卌) in B group were more than those in A group, and there was significant difference in both groups (P<0.05). The peak flow and mean flow increased after treatment in both group, and there was statistically differences. There was no statistically differences after treatment between A and B group, presenting similarities in peak flow and mean flow. Conclusion: Shaofu Zhuyu soup can significantly improve the clinical effect of Chronic abacterial prostatitis/chronic pelvic pain syndrome, and the effective time was significantly shorter.关键词：harnal capsule (tamsulosin);chronic abacterial prostatitis/chronic pelvic pain syndrome;Shaofu Zhuyu soup
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the curative effects and safety of olanzapine combined with Niuhuang Ningong tablet in treatment-resistant schizophrenia. Method: One hundred and twelve patients of treatment-resistant schizophrenia were randomly divided into observation and control groups. Olanzapine was taken oral in control group(n=56) for 8 weeks,the starting dose was 5 mg·d-1,and added to 10 mg·d-1 in weeks.56 patients of observation group took olanzapine combined with Niuhuang Ningong tablet for 8 weeks,the dose of Niuhuang Ningong tablet were 5 piece/times,tid.Clinical effectiveness and side effects were assessed with positive and negative symptom scale(PANSS),global assessment scale(GAS)and treatment emergent symptom scale(TESS)before treatment and at the end of 8 weeks of treatment. Result: The total effective rate of control group was 62.50%,and the and the total effective rate of observation group was 75.00%. the treatment TCM of pathogenic fire derived from stagnation of liver-QI, innter disturbance by phlegmatic fire (IDPF) and exuberant fire due to yin-depletion (EFYD) in control group were (2.51±0.27),(2.68±0.32),(2.47±0.39);the treatment TCM of pathogenic fire derived from stagnation of liver-QI, innter disturbance by phlegmatic fire (IDPF) and exuberant fire due to yin-depletion (EFYD) in observation group were (1.35±0.21),(1.28±0.13),(1.87±0.42).There were more significant difference in the total effective rate and after treatment Chinese Medicine symptom between observation group and the control group(P<0.05 or P<0.01).Compare with the control group, PANSS score, positive symptoms points, negative symptoms points, psychopathological points and CGI-SI total score of the observation group were significantly decreased from the fourth treatment (P<0.05 or P<0.01). The scores of TESS and the recurrence rate of the control group were (0.72±0.23)and 32.43%,and the scores of TESS and the recurrence rate of the observation group were(0.54±0.16)and 21.43%.So the adverse reaction and the recurrence rate of the observation group were significantly lower than the control group(P<0.05 or P<0.01). Conclusion: The results suggest olanzapine together with Niuhuang Ninggong tablet has good efficacy, less side effects and less recurrence rate in the treatment of treatment-resistant schizophrenia.This method deserves extensive application in the clinical.关键词：olanzapine;Niuhuang Ningong tablet;treatment-resistant schizophrenia
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Yinqiao powder combined with Huopu Xialing decoction in the treatment of children with hand-foot-mouth disease. Method: The study in 2010 March-2012 year in December will be admitted to the hand-foot-mouth disease were randomly divided into study group and control group, the control group treated by ribavirin, while the study group was given in control of Yinqiao powder plus Huopu Xialing decoction in the treatment group on the basis of treatment, compared two groups of patients with clinical symptoms, clinical remission curative effect and adverse reaction. Result: In the study group were significant efficiency and the total effective rate was significantly higher than that of the control group (P<0.05). Study group were significant effective rate and total effective rate were significantly higher than those in the control group, and the difference was statistically significant (P<0.05). Two groups were no obvious adverse reaction. Conclusion: On the base of ribavirin antiviral therapy with Yinqiao powder plus Huopu Xialing decoction in the treatment of children with hand foot and mouth disease is right in the pathogenesis, and according to the illness on the basis of addition and subtraction with the disease, can significantly improve the clinical treatment effect, the pain of patients with clinical symptoms significantly reduced, reduce the incidence of complications. The clinical application is safe and effective, worthy of clinical use.关键词：Yinqiao powder;Huopu Xialing decoction;children;hand-foot-mouth disease
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of traditional Chinese medicine decoction combined with Xueshuantong in treatment of vitreous hemorrhage. Method: This study in 2011 January-2012 year in December admitted to the vitreous hemorrhage were randomly divided into study group and control group. The study group was treated with Chinese medicine decoction (Xuefu Zhuyu decoction) combined with Xueshuantong, and the control group were treated by Xueshuantong. The clinical efficacy and therapy time were compared between the two group. Result: After treatment, visual acuity of the study group was better than in the control group (P<0.05). Total efficiency of the study group was significantly higher than that of the control group (P<0.05). The average treatment time of the study group was shorter than the control group (P<0.05). Conclusion: Xueshuantong injection combined with syndrome differentiation of traditional Chinese medicine decoction (Xuefu Zhuyu decoction) oral can promote the absorption of vitreous hemorrhage, accelerate the absorption and accumulation of blood in the recovery of visual acuity, had curative effect, and was worthy of clinical use.关键词：Xuefu Zhuyu Decoction;xueshuantong;vitreous hemorrhage
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effects of Yangfei Baoyuan capsule on stablestage patients with chronic obstructive pulmonary disease (COPD). Method: one hundred and twenty subjects were randomly divided into observation group and control group (n=60 each). Both groups were adopted the conventional drug therapy. The observation group was given Yangfei Baoyuan capsule,treatment for 3 months.The lung function, BODE index and 1 year attack frequency of acute exacexbation of chronic obstructive pulmonary disease(AECOPD) were measured to evaluated the quality of life. Result: The observation group showed significant improvement compared with the control group in the following indexes:forced espiratory volume in 1 second(FEV1),modified medical research council scale (mMRC) and BODE index were more lower than those in the control group (P<0.05).1 year number of AECOPD and hospital treatment is superior to the control group. Conclusion: Yangfei Baoyuan capsule can significantly improve the lung function,AECOPD and BODE index in patients with COPD,and treatment of patients with COPD has significant curative effect.关键词：chronic obstructive pulmonary disease(COPD);Yangfei Baoyuan capsule;lung and kidney deficiency;stablestage
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Explore the principle of pattern-herbs of Chinese medicine on coronary heart disease (CHD)by text mining analysis. Method: Download the literatures about CHD from Chinese Bio-Medical Literature Database. Mine the rules of traditional Chinese medicine(TCM) pattern and Chinese herbal medicines by data slicing algorithm. The results are showed visually with Cytoscape 2.8 software. Result: The pattern of qi deficiency and blood stasis is the main TCM symptom. Heart blood stasis syndrome and deficiency of qi and yin are the common pattern. The center TCM herbs of CHD are Radix Salviae Miltiorrhizae, Radix Astragali, Radix Puerariae, Radix Ginseng, Flos Carthami, Rhizoma Chuanxiong, Radix Notoginseng, Radix Ophiopogonis. As is shown in directional mining, the center herbs for damp-heat pattern are similar with those treatments for CHD. Conclusion: Text mining approach provides an important method in exploring the rules of pattern-herbs of Chinese medicine.关键词：text mining;data slicing algorithm;coronary heart disease;syndrome of traditional Chinese medicine
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate the effects and safety of Zhengqing Fengtongning for ankylosing spondylitis.Method: Such database as PUBMED, EMBASE-ASP,Cochrane Library,OVID,Springer-link CNKI, VIP, Wanfang and CBM, ect, were searched from the date of their establishment to January 2013.The quality of included studies was evaluated, and meta-analysis was conducted with RevMan 5.1 software. Result: A total of 12 RCTs involving 1 112 patients were included. Meta-analysis showed that there were significant differences between the Zhengqing Fengtongning and western medicines groups in the effect on improving the total effective[MD=3.07,95%CI(1.88,5.02)],total body pain[MD=-0.43,95%CI(-0.61,-0.24)],spinal pain[MD=-0.31,95%CI(-0.44,-0.19)].Zhengqing Fengtongning injection would improved the total effective[OR=4.10,95%CI(1.73,9.71)], VAS score[MD=-2.84,95%CI(-3.68,-2.00)].Zhengqing Fengtongning injection caused a higher incidence of allergic skin. Conclusion: Use high quality clinical trials to further prove the clinical effects and safety of Zhengqing Fengtongning for ankylosing spondylitis(AS) is necessary promptly.关键词：ankylosing spondylitis;Zhengqing Fengtongning;randomized controlled trials;systematic reviews
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Fibrotic disorder seriously affect the tissues and organs function. Currently prevention and therapeutic on fibrotic disorder has become a hot and focus. Traditional chinese drug become increasingly important in anti-fibrosis for its advantages, it involves multiple mechanisms of prevention and intervention of fibrotic disorder and intervents the different stages of the formation of fibrosis for its multicomponent. The progres s of single herbs and active ingredients, complex prescription in preventing and treating fibrosis, including infection of fibroblasts,cytokine,extracellular matrix are reviewed in this paper.In recent years,practice has proved that traditional chinese medicine has a good effect on the prevention of fibrosis.It is possible to discover and screen ideal antifibrotic agents with the development of modern biotechnology.关键词：tissues and organs;fibrosis;mechanism
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：The apigenin widely exist in natural botanicals anticancer drugs, apigenin anti-tumor mechanism has made important progress and breakthroughs, mainly through antioxidant and tumor-associated gene regulation, cell cycle arrest, and inhibition of tumor cell signal transduction pathway, anti-tumor invasion and metastasis, put chemosensitizing chemoprevention ways to play the anti-tumor effect. And anti-tumor mechanism proposed from the autophagy point of in-depth study of apigenin, experimental and clinical significance.关键词：apigenin;tumor;anti-tumor mechanism;autophagy
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：At present, production, management and application units of Chinese herbal pieces were no unified, standardized grading evaluation criterion, the character properties are still the main standard of traditional classification and quality evaluation. However, the diversification of raw materials sources and the changes in planting mode weakened the authority and reliability of the traditional classification and evaluation method. So, it is necessary to carry out applying basic research of specifications and quality evaluation criteria of Chinese herbal pieces, make the traditional method combine with modern method and to establish a scientific and practical grading and quality evaluating method. At the same time, the quality and clinical curative effect of Chinese herbal pieces will be greatly guaranteed. Establishment and application of classification and quality evaluation methods of Chinese herbal pieces, will provide the policy support for higher prices for better quality management, and will greatly promote the healthy development of medicine industry.关键词：Specifications of Chinese Herbal Pieces;classification method;quality of Chinese herbal pieces;higher prices for better quality management
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0