最新刊期
卷 19 ， 期 22 ， 2013
- 摘要：Objective: To reveal variation regular patterns in odor of unprocessed and processed Herba Siegesbeckiae. Method: Evaporability composition of unprocessed and processed Herba Siegesbeckiae was sampled from headspace of emanating and then response values were obtained.Discriminant factor analysis(DFA) and soft independent modeling of class analogy(SIMCA) were used to analyze characteristic parameters. Result: Electronic nose detection methods of unprocessed and processed Herba Siegesbeckiae were established,synthesis dry air supplied by air generators as carrier gas,flow rate 150 mL·min-1,headspace temperature 60 ℃,time 30 min,agitation speed 250 r·min-1,injection volume 2 mL,acquisition time 2 min,lag time 6 min.Discrepancy rate of response values from sensor LY2/gCT in odor of unprocessed and processed Herba Siegesbeckiae was -82%;Odor of unprocessed and processed Herba Siegesbeckiae could be divided into two areas by DFA discriminant model,the left area was unprocessed products,the right area was processed products;SIMCA was effective model,score was 92 by cross-validation. Conclusion: Odor of unprocessed and processed Herba Siegesbeckiae had a significant difference,and the difference could be digitalized according to odor characteristic parameters tested with electronic nose.关键词：Herba Siegesbeckiae;electronic nose;processing technique;odor;quality standardization;principal component analysis
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of hypoglycemic effective parts from Anemarrhenae Rhizoma and identify its ingredients. Method: With yield of total saponins and extract yield as indexes,uniform design test was adopted to investigate effects of ethanol concentration,ethanol amount and extraction on technology;With yield of total saponins and xanthones as indexes,extraction times was screened by single factor test.The content of xanthones was determined by UV,while total saponins was determined by HPLC,main components of hypoglycemic effective parts from Anemarrhenae Rhizoma were identified by UPLC/Q-TOF-MS. Result: Optimal extracting technology was as following:extracted twice with eight times the amount of 80% ethanol for 1 h per time;Main components of hypoglycemic effective parts from Anemarrhenae Rhizoma included 43 timosaponins and 7 xanthones. Conclusion: This optimized technology was stable and feasibel,it provided a reference for drug developing of effective parts from Chinese materia medica,which were adopted to treat diabetes and its complications.关键词：Anemarrhenae Rhizoma;hypoglycemic effective parts;total saponins;xanthone;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of carbonized Benincasae Exocarpium by baking and investigate its in vitro adsorption activity of urotoxin. Method: With adsorption force of carbonized Benincasae Exocarpium on urea and urea yield as indicators,orthogonal test was adopted to investigate effects of processing temperature and time on processing technology of carbonized Benincasae Exocarpium by baking. Result: Optimum processing technology was as following:processing time 15 min,processing temperature 280 ℃;Under these conditions,average adsorption force of carbonized Benincasae Exocarpium on urea was 20.85 mg·g-1 with RSD of 2.83%. Conclusion: Carbonized Benincasae Exocarpium by baking had in vitro adsorption activities for urea nitrogen,creatinine and uric acid.It could be used for preparation of medicines and health products,and increase medicinal value of Benincasae Exocarpium as a new source in development of Chinese medicine preparations for treatment of renal failure with advantages of inexpensive and safe.关键词：carbonized Benincasae Exocarpium by baking;processing technology;adsorption activity for toxin;adsorption force
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Qingzao Jiufei decoction by multi-index comprehensive evaluation method. Method: L9(34) orthogonal design was adopted,with dry extract rate,the contents of amygdalin,chlorogenic acid and glycyrrhizic acid as comprehensive evaluation index,effects of ethanol concentration,decocting time and times on extraction technology were investigated by composite score method.The contents of three index ingredients were determined by HPLC,chromatographic conditions were as follows:Agilent TC-C18 column(4.6 mm×250 mm,5 μm),detection wavelength program(0-50 min,207 nm;50-100 min,237 nm),column temperature 30 ℃,flow rate 1.0 mL·min-1,mobile phase of acetonitrile(A)-0.1% phosphoric acid(B) gradient elution(0-30 min,5%-10%A;30-55 min,10%-20%A;55-80 min,20%-35%A). Result: Optimal extraction technology was as following:extracted 2 times with 8 times the amount of 70% ethanol for 2 h per time. Conclusion: This optimized extraction technology was stable,feasible and reasonable with high extraction efficiency of active ingredients.关键词：Qingzao Jiufei decoction;orthogonal test;amygdalin;chlorogenic acid;glycyrrhizic acid;extract yield;wavelength switching method
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发布时间：2024-01-04 - 摘要：Objective: To evaluate anti-platelet aggregation of 6,7-dehydroroyleanone in roots of Salvia deserta and optimize its extraction process. Method: In vitro anti-platelet aggregation of 6,7-dehydroroyleanone was determined by turbidimetry.Based on single factor tests,orthogonal test was adopted to effects of extracting times,solid-liquid ratio,extracting time on extraction technology of 6,7-dehydroroyleanone with yield of 6,7-dehydroroyleanone as index.The content of 6,7-dehydroroyleanone was determined by HPLC with mobile phase of methanol-0.02% formic acid(83: 17),detection wavelength of 272 nm,column temperature at 40 ℃. Result: By comparing with the saline group,the high and middle dose group of 6,7-dehydroroyleanone could effectively inhibit platelet aggregation induced by thrombin,platelet aggregation inhibition rates of the high dose group of 6,7-dehydroroyleanone within 15,30,45,60 min were 90%,75%,66%,64%,respectively.Optimum extraction process was as following:extracted 3 times with ten-fold the amount of 95% ethanol at 85 ℃ for 3 h each time;The content of 6,7-dehydroroyleanone in roots of S. deserta was 0.01%. Conclusion: 6,7-Dehydroroyleanone in roots of S. deserta showed better anti-platelet aggregation effect,optimized extraction technology was stable,reasonable and feasible which based on the activity.关键词：Salvia deserta;6,7-dehydroroyleanone;orthogonal test;extraction technology;thrombin;anti-platelet aggregation;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To investigate pretreatment and purification processes of total flavonoids from Scutellariae Barbatae Herba with AB-8 macroporous adsorption resin. Method: Pretreatment process of total flavonoids extract from Scutellariae Barbatae Herba was investigated with transfer rate of scutellarin,clarity and solution color as indexes.With transfer rate of scutellarin and dry extract yield as indexes,single factor tests were adopted to optimize purification technology of total flavonoids from Scutellariae Barbatae Herba with AB-8 macroporous resin. Result: Transfer rate of scutellarin was up to 90% after pretreated by adjusting pH of extract solution to 3.0-3.3 and centrifuging two times.Optimum purification process parameters of total flavonoids with AB-8 resin were as follows:diameter-height ratio 1: 5,the concentration of sample solution 0.17 g·mL-1,sample volume (equivalent as crude drug)-resin volume ratio 1: 2,sampling flow rate 2 BV·h-1,elution volume with water 4.5 BV,elution solvent 40% ethanol,elution volume with 40% ethanol 5.5 BV,elution velocity 4 BV·h-1;Under these conditions,transferring rate of scutellarin was about 93% with the mass fraction of 8%,dry extract yield was 2.5%. Conclusion: Extract yield and color was significantly reduced after pretreated extraction solution of total flavonoids,and optimized purification process was reasonable,feasible and suitable for industrial production of total flavonoids from Scutellariae Barbatae Herba.关键词：Scutellariae Barbatae Herba;macroporous adsorption resin;purification technology;scutellarin;single factor test;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize prescription of total glycosides sustained-release tablets of Sanqing Jiangtang decoction,then investigate its in vitro release profile and pharmacokinetics in rabbits. Method: With hydroxypropyl methyl cellulose(HPMC) as matrix material,sustained-release tablets was prepared by wet granulation tableting technique.With cumulative release rates in 1,4,8,12 h as comprehensive evaluation index,orthogonal test was adopted to investigate effects of HPMC amount,microcrystalline cellulose(MCC) consumption and starch-lactose on formulation technology,in vitro release and in vivo pharmacokinetics in rabbits of these prepared sustained-release tablets were determined.HPLC was used to determine the content of baicalin,mobile phase of methanol-0.4% phosphoric acid(pH adjusted to 3.0 by triethylamine,48: 52),detection wavelength 270 nm. Result: Optimum prescription was the mass fraction of HPMC-K4M 20%,MCC 1%,starch and lactose as filler with dosage ratio of 3: 1,5% PVP ethanol as binders;Cumulative release rate of these prepared sustained-release tablets was>90% in 12 h.In vivo release of baicalin fitted well with single compartment model,tmax,Cmax,AUC of two preparations had significant differences. Conclusion: A good correction of in vitro release and in vivo absorption of total glycosides sustained release tablets prepared as optimized prescription was satisfied,the relative bioavailability significantly increased,which reached requirements of sustained-release formulations.关键词：Sanqing Jiangtang decoction;sustained-release tablets;orthogonal test;in vitro release;in vitro-in vivo correlation;pharmacokinetic study
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发布时间：2024-01-04 - 摘要：Objective: Through analysis HPLC fibgerprint and pharmacological effect data of different processed products of Cyperi Rhizoma,application PLS and BP-ANN model to associate total peak area of HPLC fingerprint and pharmacodynamics effect of anti-dysmenorrhea from different processed products of Cyperi Rhizoma,then to screen major effective ingredients of Cyperi Rhizoma. Method: Using PLS for data processing,BP-ANN model was established by the neural network toolbox in MATLAB,MIV of various factors were calculated,relative importance sequence of various factors for corresponding variable(pharmacological effect) was listed by according to MIV,then to screen major effective ingredients of anti-dysmenorrhea in Cyperi Rhizoma. Result: There were sixteen common peaks in eleven groups of Cyperi Rhizoma samples,compositions of each peak were assumed X1,X2,X3,X4,X5,X6,X7,X8,X9,X10,X11,X12,X13,X15,X16.Sequence of major effective ingredients for effect of Cyperi Rhizoma on writhing inhibition rate in mice dysmenorrhea model was X13,X15,X7,X16,sequence of major effective ingredients on muscle tension inhibition rate in mice isolated uterine contraction model was X15,X13,X5,X16,which were induced by oxytocin. Conclusion: Through relevance analysis of HPLC fingerprint total peak and pharmacodynamics effect by PLS and BP-ANN model,it was able to screen major effective ingredients in Cyperi Rhizoma.关键词：Cyperi Rhizoma;MIV;BP-ANN model;effective ingredients;artificial neural network
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发布时间：2024-01-04 - 摘要：Objective: To optimize ethanol extraction technology of Yinchen Siling granules. Method: With yield of geniposide as index,single factor test was adopted to screen ethanol extraction method,such as refluxing method,ultrasonic method and percolation method;Effects of ethanol concentration,soaking time,ethanol amount and percolation velocity on extraction technology was investigated by orthogonal design.The content of geniposide was determined by HPLC,chromatographic conditions were as follows:UltimateTMC18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(15: 85),flow rate 1 mL·min-1,column temperature 40 ℃,detection wavelength 238 nm,injection volume 10 μL. Result: Ethanol percolation method was adopted,the best extraction conditions were as follows:soaked 12 h by adding 10 times the amount of 70% ethanol with percolation velocity of 5 mL·min-1;Under these conditions,yield of geniposide was up to 42.524 4 mg·g-1. Conclusion: This optimized extraction process was stable,reasonable and practical,it could provide a theoretical basis for industrial production of Yinchen Siling granules.关键词：percolation extraction method;orthogonal design;HPLC;geniposide;Yinchen Siling granules;Gardeniae Fructus;Alismatis Rhizoma
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发布时间：2024-01-04 - 摘要：Objective: To optimize bio-solid bidirectional fermentation conditions of Trametes robiniophia for Isatidis Radix and determine fermentation terminal. Method: Dependent variable was the content of polysaccharides,while independent variables were temperature,moisture and inoculation amount,response surface methodology was used to optimize bio-solid bidirectional fermentation conditions;Then fermentation terminal was defined.The content of polysaccharides was determined by UV. Result: Optimal fermentation conditions was as following:temperature 29.4 ℃,moisture 75.5%,inoculation amount 0.826 mL·g-1,bias between the observed value(11.496%) and the predicted value(11.548%) of the content of polysaccharide was 0.45%,regression coefficients of binomial fitting complex model was as high as 0.982 6;Terminal of bio-solid fermentation was 20 d. Conclusion: Response surface methodology could be used to optimize bio-solid fermentation conditions of T. robiniophia for Isatidis Radix with good prediction.关键词：Trametes robiniophia;Isatidis Radix;bio-solid fermentation;response surface methodology;fermentation terminal;UV
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发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasonic-assisted alkali extraction and acid precipitation technology of total flavonoids from Acalypha australis. Method: UV spectrophotometry was used to determine the content of total flavonoids with rutin as a control component.With yield of total flavonoids as index,based on single factor test,effects of solid-liquid ratio,pH of alkaline extraction solvent,temperature,acid precipitation pH and time on extraction technology were investigated by orthogonal test. Result: The best extraction technology was as following:solid-liquid ratio of 1: 20,pH of extraction solvent 11,extracting temperature of 60 ℃,acid precipitation pH of 2.5,and ultrasonic time of 90 min.Under these conditions,extraction amount of total flavonoids from A. australis was 24.11 mg·g-1,and its yield could reach 87.76%. Conclusion: This optimized technology was stable and feasible with high extraction rate,it could provide experimental basis for development and utilization of A. australis.关键词：Acalypha australis;total flavonoids;single factor test;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To optimize microwave-assisted extraction technology of Pingtang capsules B. Method: Pingtang capsules B was extracted by microwave assisted technology,with yield of geniposide as index,choosing microwave power,material-liquid ratio,extraction time,extraction times as investigating factors,orthogonal test was adopted to optimize microwave assisted extraction technology.The content of geniposide was determined by HPLC,chromatographic conditions were as follows:DiamonsilTM RP C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(11: 89),flow rate 1.0 mL·min-1,detection wavelength 238 nm,column temperature at room temperature,injection volume 20 μL. Result: Optimum extraction technology conditions were as follows:microwave power 15 kW,solid-liquid rate 1: 5,extraction time 20 min,extraction 2 times;Under these conditions, average yield of geniposide was 9.08 mg·g-1. Conclusion: Optimized microwave assisted extraction technology had advantages of high efficiency,energy saving and time saving with great application value for industrial production of Pingtang capsules B.关键词：orthogonal test;Pingtang capsules B;microwave extraction technology;geniposide;single factor test;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize percolation process of Strychni Semen from Yaotongning plasters. Method: With transferring rate of strychnine as index,orthogonal test was adopted to optimize percolation extraction technology with the concentration and amount of ethanol,percolation rate as factors.The content of strychnine was determined by HPLC,chromatographic conditions were as follows:mobile phase of methanol-water-acetic acid(20: 96: 7,pH was adjusted to 3.10 with triethylamine),flow rate 1.0 mL·min-1,detection wavelength 254 nm. Result: Optimum percolation process was added 15 times the amount of 70% ethanol with percolation speed of 2 mL·kg-1·min-1.Under these conditions,transferring rate of strychnine was more than 90%. Conclusion: Optimized extraction technology was reasonable,convenient and feasible,this study had guiding significance for industrial production of Yaotongning plasters.关键词：Strychni Semen;percolation;orthogonal test;extraction technology;strychnine;content determination;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasound-assisted extraction technology of polysaccharides from Amomum villosum by response surface analysis. Method: On the basis of single-factor tests,with yield of polysaccharides from A. villosum as index,quadratic polynomial regression equation of polysaccharides yield was established by a three-factor and three-level Box-Behnken Design with solid-liquid ratio,extraction time and temperature as factors,then the established model was analyzed by response surface methodology to obtain optimum extraction process.The content of polysaccharides was determined by UV. Result: Optimum extraction conditions were as follows:extracting time 71 min,solid-liquid ratio 39: 1 and extraction temperature 78 ℃.Under these conditions,the estimated and observed values of yield of polysaccharides were 13.22% and (13.04±0.36)%,respectively. Conclusion: Compared with traditional water extraction,ultrasound-assisted method had advantages of time-saving and efficient,this study could provide experimental basis for the follow-up research and development of polysaccharides in A. villosum.关键词：Amomum villosum;polysaccharides;ultrasound-assisted extraction technology;response surface methodology;UV
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of compound Biqing tablets. Method: Based on single factor tests,orthogonal design was adopted to optimize ethanol extraction technology of Smilacis Chinae Rhizoma and other six herbs with composite score of diosgenin content,colchicine content and extract yield as index;With extract yield and the content of total polyphenols as comprehensive evaluation index,water extraction technology of Phyllanthi Fructus and other five herbs was optimized by orthogonal test.The contents of diosgenin and colchicine were determined by HPLC,chromatographic conditions were as follows:Cosmosil C18 column(4.6 mm×250 mm,5 μm),column temperature 35 ℃,flow rate 1.0 mL·min-1,injection volume 10 μL,detection wavelength at 203,350 nm,mobile phase with methanol-water(90: 10) and methanol-water(37: 63);The content of total polyphenols was determined by UV. Result: Optimum ethanol extraction technology was as following:extracted 2 times with 10 times the amount of 70% ethanol,1 h per time;Optimum water extraction technology was:extracted 3 times with 10 times the amount of water,1 h each time. Conclusion: This optimized technology was stable and feasible,it could provide an experimental basis for quality control of compound Biqing tablets.关键词：diosgenin;colchicine;total polyphenols;orthogonal test;extraction technology;HPLC;UV
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发布时间：2024-01-04 - 摘要：Objective: To prepare four saponin extracts from Astragali Radix,and investigated feasibility of saponin extracts instead of astragaloside IV as control components in determination of total saponins in Astragali Radix. Method: HPLC-ELSD was adopted,separation was performed on a phenomenex C18 column(4.6 mm×250 mm,5 μm),mobile phase consisted of acetonitrile and 0.3% formic acid with gradient elulion.Determination of four saponin extracts from Astragali Radix was established,and compared with external standard assay. Result: In methodological study of four saponin extracts from Astragali Radix,linear ranges of astragalosideⅠ,astragaloside Ⅱ,astragaloside Ⅲ and astragaloside Ⅳ were 50.6-810,54.3-869,56.2-899,58.3-934 mg·L-1,respectively;Average recoveries were 99.9%,100.1%,99.8%,99.8% with RSD were 0.4%,0.4%,0.7%,0.4%,respectively.Relative deviation between external standard assay and determination of four saponin extracts from Astragali Radix was less than 3.0%. Conclusion: Four saponin extracts from Astragali Radix could be applied for determining the content of total saponins from Astragali Radix,this method was simple,convenient and fulfilled method validation.关键词：HPLC-ELSD;saponin extracts from Astragali Radix;determination;external standard assay;methodological study
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC characteristic fingerprint of Jiagasong decoction. Method: Taking 6-gingerol and dehydrodiisoeugenol as control components,which were derermined by HPLC,chromatographic conditions were as follows:Platisil ODS column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-methanol-0.3% phosphoric acid solution for gradient elution,detection wavelength 274 nm,column temperature 25 ℃,flow rate 1.0 mL·min-1,injection volume 10 μL.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004A) was used in analysis 10 batches of Jiagasong decoction. Result: HPLC characteristic fingerprint of Jiagasong decoction was established,15 common peaks were received,two of them were identified as 6-gingerol and dehydrodiisoeugenol.Similarity of fingerprint among 10 batches of samples was more than 0.9,indicating a good correlation and basically the same chemical compositions. Conclusion: This assay method was simple,sensitive and accurate,this paper could provide scientific basis for quality evaluation of Jiagasong decoction.关键词：Jiagasong decoction;characteristic fingerprint;6-gingerol;dehydrodiisoeugenol;HPLC;Zingiberis Rhizoma;Myristicae Semen;Amomi Fructus Rotundus
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determining sodium danshensu and calycosin-7-glucoside in Yixin Tongmai mixtures. Method: HPLC conditions of sodium danshensu were as follows:Welchrom-C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.5% acetic acid(12: 88),flow rate 1.0 mL·min-1,column temperature 30 ℃,injection volume 10 μL,detection wavelength 280 nm.But conditions of calycosin-7-glucoside were:mobile phase of acetonitrile(A)-0.2% formic acid solution(B) with gradient elution program(0-13 min,13%-15%A;13-23 min,15%-20%A;23-30 min,20%-40%A;30-40 min,40%A),detection wavelength at 260 nm;Other conditions were the same as sodium danshensu. Result: Linear ranges of sodium danshensu and calycosin-7-glucoside were 0.213 44-5.336 and 0.078 72-2.361 6 μg,respectively;Average recoveries of them were 100.17%(RSD 2.02%) and 101.79%(RSD 1.40%),respectively. Conclusion: This established HPLC method was rapid,accurate and reproducible,it could be used for quality control of Yixin Tongmai mixtures.关键词：Yixin Tongmai mixtures;sodium danshensu;calycosin-7-glucoside;HPLC;methodological study
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发布时间：2024-01-04 - 摘要：Objective: To develop the qualitative and quantitative methods for quality control of Mushu pills. Method: TLC were used as qualitative identifications of Paeoniae Radix Alba and Corydalis Rhizoma in Mushu pills;And to develop a HPLC method for determination of paeoniflorin in Mushu pills,separation was performed on ZORBAX SB-C18 column(4.6 mm×150 mm,5μm)with acetonitrile-0.1% phosphoric acid(15: 85) as mobile phase,flow rate of 1.0 mL·min-1,detection wavelength was at 230 nm,injection volume 10 μL,column temperature was 30 ℃. Result: TLC characteristic identification was distinct and highly specific.Paeoniflorin showed a good liner relationship within the range of 37.80-756.00 ng,the average recovery was 98.2% with RSD 1.4%. Conclusion: This established determination method was reliable,accurate and specific,it could be used for quality control of Mushu pills.关键词：Mushu pills;Paeoniae Radix Alba;Corydalis Rhizoma;paeoniflorin;TLC identification;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To investigated effects of different harvesting times and different processing methods on the contents of caffeic acid and rosmarinic acid from Isodon lophanthoides. Method: The contents of caffeic acid and rosmarinic acid were determined by HPLC,which was performed on a Dikma Diamonsil C18(2) column(4.6 mm×250 mm,5 μm),gradient elution(0-17 min,12%A;17-20 min,12%-25%A;20-50 min,25%A) with mobile phase of acetonitrile-0.3% phosphoric acid,flow rate was 1.0 mL·min-1,column temperature was room temperature,detection wavelength was 329 nm,injection volume 20 μL.With the contents of caffeic acid and rosmarinic acid as indexes,effects of harvesting times(May to November) and processing methods(drying in shade,sun-dried,drying) on quality of I. lophanthoides were investigated. Result: Different harvesting times had little effect on the content of caffeic acid from stems and leaves of I. lophanthoides,but it had significant effect on the content of rosmarinic acid.After handled by three drying methods,yield of rosmarinic acid were 1.365%,1.790%,1.577%,respectively;Yield of caffeic acid were 0.057%,0.053%,0.055%,respectively. Conclusion: I. Lophanthoides harvested in July had the best quality,herbs could be harvested twice a year,harvesting times were in July and November;Processing method of naturally dried was optimum.关键词：HPLC;content determination;Isodon lophanthoides;different processing methods;different harvesting times
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发布时间：2024-01-04 - 摘要：Objective: To find the correlative characteristic components of Angelicae Dahuricae Radix which is related to sulfur fumigated. Method: Normal or reversed phase partition chromatography, mass spectrum and nuclear magnetic resonance spectroscopy were used for isolation and identification of compounds. Using high-performance liquid chromatography(HPLC) analysised. Result: Thirteen compounds were obtained and identified as oxypeucedanin(1), phellopterin(2), xanthotoxin(3), oxypeucedanin hydrate(4), pabulenol(5), marmesin(6), byakangelicin(7), bergapten(8), isopimpinellin(9), imperatorin(10), isoimperatorin(11), byakangelicol(12) and neobyakangelicol(13). Oxypeucedanin, oxypeucedanin hydrate,bergapten and byakangelicol were obviously changed in HPLC fringerprints after fumigated with sulfur. Conclusion: The quality control of Angelicae Dahuricae Radix provide new evidence for the selection of indicators.关键词：Angelicae Dahuricae Radix;oxypeucedanin;fingerprint;quality of medicinal materials
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method of synephrine, norisoboldine and arecoline in Simo Tang oral liquid by HPLC. Method: HPLC method was set up, column was Agilent SCX, the mobile phase was methanol-0.2% phosphate solution, with the flow rate of 1.0 mL·min-1;the detection wavelength was set at 215 nm, column temperature was kept at 35 ℃. Result: Synephrine was linear within the range of 0.05-2.55 μg (r=0.999 9);the average recovery was 99.8%. Norisoboldine was linear within the range of 0.04-2.01 μg (r=0.999 9); the average recovery was 100.61%. Arecoline was linear within the range of 0.13-2.59 μg (r=0.999 8); the average recovery was 100.6%. Conclusion: The method is simple, accurate and stable, which is valuable to improve the quality standard of Simo Tang oral liquid.关键词：HPLC;Simo Tang oral liquid;synephrine;norisoboldine;arecoline
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发布时间：2024-01-04 - 摘要：Objective: To analyze the contents of tannin and the absorption capacity of carbon between Scutellaria Radix and charred Scutellaria Radix so as to explore the mechanism of the hemostatic effect of charred Scutellaria Radix. Method: Phosphotungstomolybdate acid/casein-spectrophotometry and methylene blue-spectrophotometry were adopted to compare the contents of tannin and the absorption capacity of carbon. Result: After being charred, the content of tannin significantly decreased but the absorption capacity of carbon significantly increased. Conclusion: The influence of tannin on the mechanism of the hemostatic effect of charred Scutellaria Radix was not consistent with that of the adsorption capacity of carbon. The deeply integrated research on chemical substances will be considered to clarify the mechanism of hemostatic effect of charred Scutellaria Radix.关键词：Scutellaria baicalensis;fried into carbon;variation discipline;the theory of processing
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the stems of Homalium paniculiflorum. Method: The compounds were isolated by many kinds of chromatography methods and identified on the basis of physico-chemical characters and spectroscopic analysis. Result: Thirteen compounds were obtained from the EtoAc extracts of the stems of H. paniculiflorum, and their structures were identified as 4-hydroxy-3-methoxybenzaldehyde(1), 2,4-dihydroxybenzaldehyde(2), 3-(phenyl)isocoumarin (3),4-hydroxy styrene aldehyde(4),4-hydroxy-3,5-dimethoxy-benzoic acid(5),kaempferol(6), arimadendrin (7), 4-hydroxy-3,5-dimethoxy styrene aldehyde (8),2-hydroxy-benzoicacid(9), 28-O-acetylbetulin (10), cholesterol(11), benzoic acid(12) and 5a-cholestan-3-one(13). Conclusion: Compounds 3,5,7-11,13 were isolated from the family Flacourtiaceae for the first time. All compounds are firstly isolated from the genus H. paniculiflorum.关键词：Homalium paniculiflorum;chemical constituents;Flacourtiaceae
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the seeds of Momordica charantia. Method: The compounds were separated and purified from the title by using a various ch-romatographic techniques including colum chromatography over silica gel, ODS and preparative HPLC.Their structures were elucidated by spectroscopic techniques including 1H-NMR,13 C-NMR,2D-NMR,ESI-MS and HRESI-MS. Result: Ten compounds were isolated from this plant and their structures were identified as 24β-ethyl-5α-cholest-7, anti-22E,25(27)-triolefin-3β-hyd-roxy-3-O-β-D-glucopyranoside(1),1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (MT CA)(2),nicotinamide(3),uracil(4), (6S,7E,9S)-6,9,10-trihydroxy-4,7-megastigmadien-3-one (5), epipinoresinol-4,4'-di-O-D-glucopyranoside(6),lectin(7),(6R,9S)roseoside(8),vicine(9), 6-(2,3-dihydroxyl-4-hydromethyl-tetrahydro-furan-1-yl)-cyclopentene[c]pyrrole-1,3-diol(10). Conclusion: The compounds 2,3,5,6,7,9,10 were isolated from the seed of M. charantia for the first time.关键词：Momordica charantia seed;chemical constituents;lignan;alkaloid;isoprene
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of twigs of Artocarpus nanchuanensis. Method: The constituents were isolated from 95% ethanol extraction of twigs of A. nanchuanensis by column chromatography over silica gel, Diaion HP20 macroreticular resin, MCI CHP 20P gel, Sephadex LH-20 gel, ODS gel, etc. Their structures were elucidated by analysis of physical chemical properties and spectral data. Result: Ten compounds were isolated and their structures were identified as diosgenin (1), prosapogenin of dioscin (2), progenin Ⅱ(3), diosgenyl-3-O-α-L-rhamnopyranosyl(1→3)-[α-L-rhamnopyranosyl (1→4)]-β-D-glucoside (4), 7-oxositosterol acilglicosilado (5), ethyl caffeate (6), dibutylphthalate (7), pinoresinol (8), (7S, 8S, 8'R)-5,5'-dimethoxylariciresinol (9), palmitic acid (10). Conclusion: Except for compounds 6 and 10, the other compounds are reported from the family Moraceae for the first time. This is the first time that 4 has been reported as naturally occurring compound.关键词：Moraceae;Artocarpus nanchuanensis;chemical constituent;spirostanol derivatives;lignanoid derivatives
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发布时间：2024-01-04 - 摘要：Objective: To study the optimal methyl esterification method in alkali alcohol eluent from Yiqi Fumai lyophilized injection. Method: Some esterification methods were adopted as follows:1% sulfuric acid-methanol refluxed esterification method, 10% sulfuric acid-methanol refluxed esterification method,25% sulfuric acid-methanol refluxed esterification method and 2% potassium hydroxide-methanol refluxed esterification method. The solution of methyl esterextraction was extracted by opportune solvent, then, analysis them with GC-MS. Result: 10% sulfuric acid-methanol refluxed esterification with extracted by chloroform is the optical method, which shows the best esterification efficiency. Conclusion: 10% sulfuric acid-methanol refluxed esterification is the best esterification method of alkali alcohol eluent from Yiqi Fumai lyophilized injection,which provides the basis for the research on the main ingredients of this extract.关键词：Yiqi Fumai lyophilized injection;methyl esterification;GC-MS analysis
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of flavonoids from Lantana camara. Method: The EtOH extracts from L. camara were initially separated by macroporous adsorptive resin, and then were isolated and purified by different column chromatographic methods. The structures of the flavonoids were identified by their physicochemical properties and spectroscopic data. Result: Nine compounds were isolated and identified as 3-methoxyquercetin (1),quercetin-3-O-β-D-glucoside (2),3,7-dimethoxyquercetin (3),luteolin-7-O-β-D-glucoside(4),5, 6, 7-trihydroxy-4'-methoxyflavanone (5),pedalitin (6),eupatilin (7),hispidulin (8),isosakuranetin (9). Conclousion: All the compounds are isolated from this plant for the first time.关键词：Lantana camara;chemical constituents;flavonoids
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发布时间：2024-01-04 -
HPLC-MS Analysis of Chemical Constituents in Sini Decoction Prepared by Different Extraction Methods
摘要：Objective: To identify the structure of the main chemical constituents in Sini decoction prepared by different extraction methods by HPLC-MS. Method: The Sini decoction was prepared by boiling water extraction method and combination of active parts method.C18 (4.6 mm×250 mm, 5 μm) column was adopted. The gradient mobile phase consisted of acetonitrile (containing 0.01% acetic acid) and 2 mmol·L-1 ammonium acetate solution (containing 0.01% acetic acid) at a flow rate of 0.6 mL·min-1; the column temperature was 30 ℃; the injection volume was 2 μL. MS conditions: positive mode, dryer flow rate: 10 L·min-1, atomizing air pressure: 30 psi, debris voltage: 70 V. Result: 28 and 30 chemical constituents were identified in traditional water decoction and active ingredients combineddecoction by HPLC-MS respectively. All of the constituents were classified according to their medicinal materials derivation. Conclusion: The experiment provide a basis for the determination of the active ingredients in Sini decoction.关键词：Sini Decoction;HPLC-MS;qualitative analysis- 3
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the badger oil for the foundation of the quality standards of the badger oil. Method: The compounds were isolated and purified by silica gel column chromatography and preparative layer chromatography methods, and their structures were identified by spectral analysis. HPLC fingerprints of Badger oil and pig oil were compared with the chromatographic conditions as follows:The Phenomenex Hyper C18with cetonitrile-dichloromethanol (75: 25) as the mobile phase was used. The column temperature was 30 ℃, the flow rate was 1.0 mL·min-1 and ELSD was used, the tube temperature was 70 ℃, the press of nitrogen was 3.5 bar. Result: Two compounds were isolated and identified as glycerol trioleate (1) and glycerol linoleate dioleate (2). HPLC fingerprint showed there is big difference in chemical constituents exits between the badger oil and the pig oil. Conclusion: Compounds 1, 2 were obtained for the first time from badger oil.关键词：badger oil;chemical constituents;glyceride;structural identification;HPLC-ELSD
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the ethyl acetate extract of Liparis nervosa. Method: Chemical constituents were isolated by Column chromatography on Silica gel and Sephadex LH-20, their structures were identified by physicochemical characters and spectroscopic analysis or comparison with standard compounds. Result: Seven compounds were isolated and identified as 2,2-dimethyl-8- (3-hydroxyisoamyl)chroman-6-carboxylic acid (1), linoleic acid (2), α-spinasterol glucoside (3), glycerol monopalmitate (4), genkwanin(5), apigenin(6), butane diacid(7). Conclusion: Compounds 1 was a new natural product, 2-7 were isolated from L. nervosa for the first time.关键词：Liparis nervosa;chemical constituents;identification
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发布时间：2024-01-04 - 摘要：Objective: To establish methods for identification and determination of caffeic acid and rosmarinic acid in Rabdosia. lophanthoides var gerardiana by TLC and HPLC. Method: The developing solvent for caffeic acid and rosmarinic acid in R. lophanthoides var gerardiana by TLC was n-hexane-ethylestate-formic acid (3: 3: 1), detected under the UV light (365 nm). HPLC method was performed on a Dikma Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase of methanol-0.3% phosphoric acid by gradient elution. The flow rate was 1.0 mL·min-1. The column temperature was kept at 25 ℃ and detection wavelength was set at 329 nm. Result: Caffeic acid and rosmarinic acid could be detected with clear spots and good separated by TLC, caffeic acid and rosmarinic acid showed good linearity in the ranges of 0.045 4-0.908 0 μg (r=0.999 9) and 0.219 2-4.384 0 μg (r=0.999 6) with average recoveries of 96.57% (RSD 1.32%) and 99.48% (RSD 2.53%). Conclusion: The methods are accurate and can be used for the quality of R. lophanthoides var gerardiana.关键词：Rabdosia lophanthoides (Buch.-Ham ex D. Don) Hara var gerardiana (Benth.) Hara;caffeic acid and rosmarinic acid;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the volatile oil from the flower bud of Prunus mume. Method: The volatile oil was extracted by steam distillation and analyzed by GC-MS. The composition of the volatile oil was determined according to peak area normalization method. Result: Total 51 compounds were isolated and identified from the volatile oil of the flower bud of P. mume. Conclusion: Chemical constituents of the volatile oil from the flower bud of P. mume included aldehydes, esters, organic acids, alcohols and fatty compounds. The main components were benzaldehyde (42.93%), benzyl benzoate (9.16%), heneicosane (7.96%), tricosane (7.39%), n-hexadecanoic acid (3.62%) and 3-allyl-6-methoxyphenol (2.51%).关键词：Flos mume;volatile oil;gas chromatography-mass spectrography (GC-MS)
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the roots of Psammosilene tunicoides. Method: The chemical constituents were isolated and purified via silica gel, Sephadex LH-20 column chromatography, RP-18 column and MPLC. Their structures were identified on basis of spectral data and chemical methods. Result: Two cycloheptapeptides were isolated from the roots of P. tunicoides, tunicyclin C (1) and tunicyclin K (2). Conclusion: Tunicyclin K was a new natural product.关键词：cycloheptapeptide;Psammosilene tunicoides;structure identification
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发布时间：2024-01-04 - 摘要：Objective: To establish assaying methods for the determination of deoxynojirimycin in silkworm. Method: The content of DNJ was determined by RP-HPLC, using a Hypersil NH2 (4.6 mm×250 mm, 5 μm). The column temperature was maintained at 30 ℃. Acetonitrile-water (78: 22) was used as mobile phase;the flow-rate was 1.0 mL·min-1. The detecting wavelength was set at 203 nm. Result: The linear range was 1.996-9.98 μg (r=0.999 6). The average recovery was 98.4%. Conclusion: This method is simple, practical and accurate. It could be used for quality control of silkworm.关键词：silkworm;deoxynojirimycin (DNJ);RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To provide scientific basis for the utilization and development of the leaves of Mangifera sylvatica by establishing tis quality control standard. Method: According to Chinese Pharmacopeia (2010 ediction),the property and microscopic characterstics of M. sylvatica,moisture,total ashes content,acid-insoluble ashes content were identified by TLC.The content of dinsenoside mangiferin in M. sylvatica was determined by HPLC. Result: Macroscopic and microscopic characteristics of M. sylvatica were described.The moisture in the leaves was below 12%,with the total ash content of 16%,the content of acid-insolubal ash was equal or below 5%.The content of hot homeopathic alcohol was above 23%.The spot of TLC were clear and well-sparated.The linear range of dinsenoside mangiferin were 0.4-1.4 μg(r=0.999 9);the average recoveries were 100.45% and RSD of them were 1.59%;the contents of mangiferin in the leaves were above 2%. Conclusion: The quality standard is suitable for the leaves of M. sylvatica.关键词：Mangifera sylvatica;quality standard;mangiferin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the volatile constituents from of Vitex negundo flowers from different regions. Method: The essential oil of V. negundo flowers Jiyuan in Henan province and Dabieshan mountain in Hubei province were analyzed by SPME-GC-MS for the first time. Result: Seventy compounds were isolated from volatiles in two different areas and forty-three were identified. Fourteen compounds were owned in both samples. β-Caryophyllene was the highest content accounted for 40.36% and 32.42% in both samples respectively, followed by cineole and (Z)-b-farnesene in mutual compounds. Conclusion: The results showed that the major components of volatiles from V. negundo flowers in different region were in closer, but the kinds and contents were different.关键词：Vitex negundo flowers;essential oil;SPME;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a fingerprint of Fufang Tujingpiding by HPLC. Method: The experiment was conducted on Agilent Zorbax SB C18(4.6 mm×250 mm, 5 μm), eluted with methanol (B)-0.09% formic acid aqueous solution (A) as the gradient elution mobile phases. The detective wavelength was set at 260 nm with the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30 ℃. The data was analyzed by Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine software to compare the quality of 14 batches of Fufang Tujingpiding. Result: The standard chromatographic fingerprints of Fufang Tujingpiding was established, 13 co-possessing peaks were marked according to the fingerprints of 14 batches of it, and the similarity was greater than 0.96. Conclusion: The established method has good precision, reproducibility and stability, which provides the basis for the quality control of Fufang Tujingpiding as well.关键词：Fufang Tujingpiding;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of iridoid glycosides capsule of Lamiophlomis rotata (IGC). Method: The iridoid glycosides capsule of L. rotata was identified by TLC. The contents of total iridoid glycosides, shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester in the capsule were determined by the first derivating UV-spectra and HPLC. Result: The identify spots were clear in TLC, and the constituents were well separated under the chromatographic condition. The contents of total iridoid glycosides of three batch capsule were more than 50%, the shanzhiside methyl ester and 8-O-acetylshanzhiside methyl ester were separated with other composition and the content were 73.96,72.43 mg·g-1 respectively. Conclusion: The method can be used to control the quality of IGC.关键词：Lamiophlomis rotata;iridoid glycosides capsule;quality standard
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of tirucallol, euphol and 24-methylene cycloartanol in Euphorbiae Pekinensis Radix and its processed products. Method: An HPLC method had been developed for detecting. The separation was performed on an Agilent XDB C8 column (4.6 mm×150 mm, 5 μm) at 35 ℃ with acetonitrile-H2O (90: 10) as the mobile phase. The flow rate was 1.0 mL·min-1, and the detection wavelength was set at 205 nm. Result: The linear response range was 0.506-5.06 μg (r=1.000 0) for tirucallol, 1.05-10.5 μg (r=0.999 9) for euphol and 1.12-11.2 μg (r=0.999 8) for 24-methylene cycloartanol. The average recoveries of these components were 99.7% (RSD 0.9%), 99.4% (RSD 1.0%) and 99.4% (RSD 1.3%) respectively. Conclusion: The method is simple, accurate and reproducible for quality control of Euphorbiae Pekinensis Radix and its processed products.关键词：Euphorbiae Pekinensis Radix;processed products;triterpenes;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC fingerprint of Ampelopsis grossedentata,and to make evaluation by principal component and cluster analysis for its quality control. Method: The HPLC analysis was performed on a UltimateXB-C18 column (4.6 mm×250 mm,5 μm) with column temperature at 40 ℃. The mobile phase was consisted of water containing 0.5% formic acid and acetonitrile in gradient mode. The detection wavelength was set at 365 nm. All data were analyzed with the SPSS statistical software. Result: The mutual mode of HPLC fingerprint has been established. There were 11 common peaks, two of which were identified. 18 samples from different clonal germplasm were classified into three groups based on principal component and cluster analysis. Conclusion: The method was time-saving and represent the chemical information and provide a scientific basis for quality control of Ampelopsis grossedentata.关键词：Ampelopsis grossedentata;HPLC fingerprint;principal component analysis;cluster analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining isofraxidan in Zhongjiefeng Fensanpian by LC-MS-MS. Method: Agilent SB-C18(2.1 mm×50 mm, 1.8 μm) column was used with methanol-H2O (35: 65) as mobilephase. The flow rate was 0.2 mL·min-1.MS-MS detection in multiple reactions monitoring (MRM) mode using positive electrospray ionization as the ionization source was applied to determine positive ions at (m/z)222.9/189.7, 222.9/161.9. Result: Isofraxidan was linear in the range of 0.48-2.40 mg·L-1 (r=0.999 2). The average recovery was 99.3%, and RSD was 0.55% (n=9). Conclusion: The method is simple, rapid, accurate and reliable, which can be used for determination of isofraxidan in Zhongjiefeng Fensanpian.关键词：Zhongjiefeng Fensanpian;isofraxidan;LC-MS-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a high performance liquid chromatography (HPLC) method to determine acetyl-11-keto-β-boswellic acid in Xiaohuoluowan (concentrated pills). Method: The determination was carried out with Inertsil ODS-3 C18(4.6 mm×250 mm, 5 μm) column. The mobile phase consisted of acetonitrile-0.1% acetic acid (80: 20). The flow rate was 1 mL·min-1. The detection wavelength was set at 249 nm. The column temperature was set at 30 ℃. The external standard method was used to quantify acetyl-11-keto-β-boswellic acid, the main chemical constituent of olibanum in Xiaohuoluowan (concentrated pills). Result: There was a good linear relationship between the absorption peak area and the concentration in the range of 25.95-207.6 mg·L-1(R2=0.999 9) for acetyl-11-keto-β-boswellic acid. The average recovery was 99.95% (n=6). Conclusion: The method is simple, accurate and reproducible. It can be used for quality control of olibanum in Xiaohuoluowan (concentrated pills).关键词：Xiaohuoluowan;acetyl-11-keto-β-boswellic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method for simultaneous determination of danshensu, protocatechuic aicd, protocatechuic aldehyde, ferulic aicd and salvianolic aicd B in Salvia miltiorrhiza Bunge f. alba, the wine fried and the carbonizing, and explore the effects for their five phenolic acids with different processing methods S. miltiorrhiza Bunge f. alba. Method: The column was C18(4.6 mm×250 mm,5 μm). The mobile phase was methanol and 5% acetic acid glacial with gradient elution and the flow rate was 1.0 mL·min-1, the detection wavelength was 286 nm. Result: The linearities were obtained over the range of 7.31- 234(R2=0.999 5) for danshensu, 6.75-216 (R2=0.999 9) for protocatechuic acid, 52.8-1.65 (R2=0.999 0) for protocatechuic aldehyde, 4.67-149.50 (R2=0.999 8) for rosmarinic acid, 42.25-1 352 mg·L-1(R2=0.999 9) for salvianolic aicd B, respectively. All of the average recoveries were>98% with RSD<1.6%. Conclusion: The validated method has the advantages of simplicity, precision and reliability, and could be used to simultaneous determination of the five phenolic acids in S.miltiorrhiza Bunge var alba. The results indicated that protocatechuic aicd wasn't detected in the three processed products, and the rest four contents presented the fresh
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of cryptotanshinone, tanshinone I tanshinone ⅡA in Salviae Miltiorrhizae Radix et Rhizoma by dual-wavelength HPLC. Method: The Agilent ZORBAX SB-C18 column(4.6 mm×250 mm, 5 μm)was used with a mobile phase of methanol-water at 1 mL·min-1, and the detection wave-lengths were set at 245 nm(tanshinone I)and 269 nm(cryptotanshinone and tanshinone ⅡA). Result: All calibration curves showed good linear regression in test concentration ranges(r>0.999 7), and the overall average recoveries were in the range of 98.0%-100.1%(n=6) with RSD no more than 3.0%. Conclusion: The results indicated that this dual-wavelength HPLC method is rapid, accuracy, and simple, which is suitable for the quality control of the main phenanthraquinone in Salviae Miltiorrhizae Radix et Rhizoma.关键词：HPLC;dual-wavelength;Cryptotanshinone;tanshinone I;Tanshinone ⅡA;determination
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发布时间：2024-01-04 - 摘要：Objective: To observe the inhibition of Lianhua Qingwen capsule(LHQW) on Staphylococcus aureus biofilm(S.a BF). Method: Microdilution method was used to assay minimal bactericidal concentration (MBC) of LHQW and penicillin. The crystal violet(CV) and microbial activity (WST) assay were used to evaluate the inhibition of LHQW and penicillin on S.a BF. The scanning electron microscopy (SEM) and laser scanning confocal microscopy (CLSM) were used for observing S.a BF morphology and thickness measurement. Result: The MBC values of LHQW and penicillin against free S.a were 20 g·L-1 and 2 mg·L-1. Compared with the control group (control), LHQW significantly inhabited S.a BF(P<0.05,P<0.01),penicillin was no significant difference;S.a BF of the control group showed the thicker,normal bacterial form, dense overlay and the same size under the SEM, while S.a BF of LHQW group showed scattered colonies, inconsistent size, and S.a BF of penicillin group showed thicker, basically the same size, and the individual bacteria were collapse;S.a BF of control group were made of viable cells and a little dead bacteria under the CLSM, while S.a BF of LHQW group had a large number of dead bacteria, with which viable cells mixed;S.a BF of penicillin group was made of viable cells and the dead bacteria are distributed over the surface of S.a BF. Compared with the blank control group, S.a BF of LHQW group was thick significantly (P<0.01),penicillin had no significant difference;Sa BF of LHQW group was less thick significantly than the penicillin group (P<0.01). Conclusion: LHQW has significant anti- S.a BF effect. Anti-S.a BF assessment system can be used in the antibacterial effect of traditional Chinese medicine.关键词：Lianhua Qingwen capsule;penicillin;staphylococcus aureus;biofilm
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective of Trichosanthis Fructus and Allium Macrostemonis Bulbus on myocardial ischemic injury and its mechanism in rats. Method: Sprague Dawley rats were divided into 6 groups.The models of chronic myocardial ischemia was established by intraperitoneal injection of isoproterenol. After two weeks,activity of superoxide dismutase (SOD), levels of lactate dehydrogenase (LDH), creatine kinase (CK) and malondialdehyde (MDA) were measured.Western blotting was used to assay effects of Trichosanthis Fructus and Allii Macrostemonis Bulbus on mitogen-activated protein kinase (MAPKs) family of 3 major signaling molecule c-Jun n-Terminal kinase (JNK), extracellular signal-regulated kinase (ERK), phosphorylation of p38. Result: Compared with normal group, LDH, CK and MDA of model group increased significantly, SOD decreased significantly(P<0.05, P<0.01). Compared with normal group, P-p38 P-JNK P-ERK of model group was significantly increased (P<0.05, P<0.01). TKAM can significantly decrease the content of MDA, CK, LDH in serum.P-p38,P-JNK,P-ERK,MAPK protein levels of ischemia myocardial tissue increased significantly, TKAM can effectively lower levels of P-p38,P-JNK,P-ERK. Conclusion: Trichosanthis Fructus and Allium Macrostemonis Bulbus has a good protective effect on myocardial ischemia injury. The mechanism may be adopted on scavenging oxygen free radicals, reduce the oxidation inhibition of p38 and JNK phosphorylation of proteins in order to reduce ischemic injury.关键词：Trichosanthis Fructus and Allium Macrostemonis Bulbus;myocardial ischemia;p38 MAPK
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发布时间：2024-01-04 - 摘要：Objective: The monoamine oxidase (MAO) activity screening model is established to guide the separation of Trionycis Carapax active peptides, and inspect the activity of the monomer. Method: Pargyline was used as positive drug with reference to the method of MAO kit to establish the monoamine oxidase activity screening model;the ethanol concentration of Trionycis Carapax water extraction liquid was adjusting to 20%, 40%, 60%, 80%, 60% in turn to get the alcohol precipitated parts, then screen the alcohol sink parts in this model, and continue the separation of optimal selection activity part, finally MAO inhibitory activity of the monomer was tested in the model. Result: The pargyline's concentration was 1.0 g·L-1,the inhibition rate on MAO was remarkable, which could amount to 98.30%-103.05%;when the concentration was at 1.0 g·L-1,the inhibitory rate of Trionycis Carapax water extraction liquid part on MAO was (23.34±9.66)%, 20% alcohol precipitated part was (28.16±5.78)%, 40% alcohol precipitated part was (30.69±7.17)%, 60% alcohol precipitated part was (41.60±8.03)%, 80% alcohol precipitated part was (64.91±2.94)%, 80% alcohol precipitated part was(53.34±7.76)%.We get the Trionycis Carapax peptide from the 90% alcohol precipitated part. The Trionycis Carapax peptide's inhibition rate on MAO was obvious when the concentration ranged from 0.1-1.6 g·L-1,which ranged from 15.30% to 54.84%. Conclusion: The pargyline's inhibitory effect on MAO is remarkable, which suggests the model is successful and can guide separation of Trionycis Carapax,which has obvious inhibitory activity on MAO.关键词：Trionycis Carapax;MAO;active peptide
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Puren Dan (PRD) recipe on the apoptosis of INS-1 cell and expression of Bal-xl/Bcl-2 associated death promoter (BAD) and forkhead box O1 (FOXO1) in high glucose condition, and explore the molecular mechanism of PRD on restoring secretion function of INS-1 cell. Method: Experimental injured INS-1 cell model was induced by high glucose (containing 33.3 mmol·L-1), and intervened by PRD drug-serum. INS-1 cells were divided into 5 groups:the control group, high glucose group, high PRD drug-serum group, low PRD drug-serum group and metformin drug-serum group. Cell viability, INS-1 cell apoptosis and expression level of related proteins and its phosphorylation were detected by CCK-8 assay, flow cytometry and AnnexinV-FITC/PI staning and Western blotting assay, respectively. Result: High glucose could decrease cell viability and induce INS-1 cell apoptosis, and it also downregulatedf phosphorylation level of BAD and FOXO1. PRD can increase cell viability, reduce apoptosis of INS-1 cell downregulated expression of FOXO1 and improved phosphorylation level of BAD and FOXO1. Conclusion: PRD drug-serum shows a protective effect on apoptosis INS-1 cell which induced by high glucose, and the mechanism may be associated with increase of the phosphorylation levels of BAD and FOXO1.关键词：Puren Dan;high glucose;INS-1 cell;apoptosis;BAD;FOXO1
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Danggui Buxue decoction on proliferation in the hypoxic vascular endothelial cells and to explore the mechanism of angiogenesis. Method: CCK-8 assay was used for the proliferation of vascular endothelial cells;ELISA assay was used for vascular endothelial growth factor (VEGF) and its receptors (VEGFR1, VEGFR2, sVEGFR1, sVEGFR2) expression of vascular endothelial cells. Result: Compared with normal group, the hypoxic endothelial cell proliferative capacity was significantly decreased (P<0.01).Danggui Buxue decoction could promote the proliferation of normal endothelial cells and hypoxic endothelial cells with dose-response relationship compared with model group (P<0.01). Danggui Buxue decoction could promote expression of VEGF in hypoxic vascular endothelial. Compared with model group, Danggui Buxue decoction could significantly up-regulate VEGF, VEGFR1,VEGFR2 expression, and showed a dose relationship,but could inhibit sVEGFR1,sVEGFR2 expression. Conclusion: Danggui Buxue decoction can promote proliferation of the hypoxic vascular endothelial cells, The mechanism might be related to regulation of VEGF and its receptors.关键词：Danggui Buxue decoction;the hypoxic vascular endothelial cells;cell proliferation;molecular mechanism
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Zhizi Baipi decoction on rat model with TCM Yang-jaundice syndrome. Method: Rat model with Yang-jaundice syndrome was induced by ig α-naphthyl isothiocyanate (APIT),along with the hot and humid environment and high-fat and high-carbohydrate diet.Several doses of Zhizi Baipi decoction extract were ig administrated to the models, the daily state of rats was observed. The expressions of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bilirubin(TBIL), bile acid(TBA), complement C3,C4, total superoxide dismutase(T-SOD), malonaldehyde(MDA) were detected and the pathological sections were observed under the light microscope. Result: Compared with model group, moderate dose of Zhizi Baipi decoction group and high dose of Zhizi Baipi decoction group decreased significantly ALT,AST,TBIL,TBA,complement C3,C4 in blood serum(P<0.01), improved the activity of SOD(P<0.05,P<0.01);moderate dose of Zhizi Baipi decoction group decreased the content of MDA (P<0.05), they reduced the pathological injury in liver tissues at different levels. Conclusion: Zhizi Baipi decoction has positive effects in treatment of TCM Yang-jaundice, the mechanism may lie in alleviating liver injury,antagonizing free radical injury and preventing inflammatory response.关键词：Zhizi Baipi decoction;Yang-jaundice syndrome;biochemical indexes;pathological sections
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effect of compound Xuelian capsule on type Ⅱ collagen-induced arthritis in rats, and to explore its mechanism. Method: Rats were given pedal intradermal injection of bovine type Ⅱ collagen emulsion, collagen-induced arthritis(CIA)model was established. To measure and observe the effect of compound Xuelian capsule on the ankle swelling of CIA rats, the synovial tissue was collected to observe the synovial histopathology of rats. The phlogistic cell factor tumor necrosis factor-α (TNF-α) and prostaglandin E2(PGE2) were detected by enzyme linked immunosorbent assay(ELISA). Result: In model group, the rat paw edema was significant compared with the normal control group(P<0.01). Compound Xuelian capsule could significantly decrease the arthritis swelling of CIA rats(P<0.05, P<0.01);in compound Xuelian capsule groups, it could improve the synovial tissue pathological damage, compared with the CIA model group, it could significantly reduce inflammatory cell infiltration and hyperemia of synovial tissue. After 14 days' administration, the level of TNF-α and PGE2 was significantly lower in the medium and high dose group of compound Xuelian capsule than that in the model group (P<0.05, P<0.01). Conclusion: Compound Xuelian capsule could inhibit the collagen-induced arthritis in rats, the mechanism action may be related to inhibiting the expression of inflammatory mediators TNF-α and PGE2.关键词：compound Xuelian capsule;collagen-induced arthritis;synovial tissue biopsy;TNF-α;PGE2
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Xiaoyao San (XYS) and its effective compositions on behavior and memory in chronic unpredictable mild stress (CUMS) model rats, so as to reveal the characteristics of different effective compositions that represent different therapeutic methods. Method: Wistar rats were randomly divided into eight groups:XYS group (30 g·kg-1), Shugan (SG) group (5.25 g·kg-1), Yangxue (YX) group (9 g·kg-1), Shugan+Jianpi (SG+JP) group (21 g·kg-1), Yangxue+Jianpi (YX+JP) group (24.75 g·kg-1), control group, model group, and amitriptyline group (10 mg·kg-1). The rat depression model was established by using chronic unpredictable mild stressors and solitary feeding condition as described in the literature. All rats were intragastrically administrated with different decoctions or distilled water for 4 weeks. Weights, preference for 1% sucrose solution (PSS), autonomic activities and Morris water maze test (MWMT) were carried out in different time. Result: Compared with model group, growth of weight, PSS, autonomic activities, as well as moving distance percentage at target quadrant and entering frequency into valid area in MWMT were significantly increased by XYS, SG, YX, SG+JP and YX+JP group. Meanwhile, the escape latency in Morris test was decreased also by those five groups above. XYS, SG and SG+JP groups were compared with YX and YX+JP groups respectively. The amelioration effects of former groups on CUMS model rats were better than that of later groups. Especially, the effects of SG group were more prominent than other groups when treated for 2 weeks. Conclusion: The decoction of XYS can improve behavior and memory in rat CUMS model, showing antidepressant effect. The SG compositions play a principal role in antidepressant effect of Xiaoyao San formula, while the YX drug-group serves as an important assistant to the antidepressant effect of Xiaoyao San.关键词：Xiaoyao San;efficacy drug-group;antidepressant effect;CUMS;behaviors
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective effects of genistein(Gen) on cerebral ischemia/reperfusion(I/R) injury and mechanism about inflammatory reaction and cytokine secretion. Method: A rat I/R injury was made through embolization of the middle cerebral artery, and rats were treated with Gen (0.75,1.5 μmol·kg-1)via sublingual vein injection. The volumes of infarction and water content in the cerebral tissue, the activities of lactate dehydrogenase (LDH) in serum and brain tissue, and the imerleukin(IL)-6, IL-1β and tumor necrosis factor-α(TNF-α) in the serum were measured. Result: Compare with the sham operation group, the rats in I/R group showed an obvious white infarction area, an increased water content(P<0.05), and decrease LDH activity(P<0.05) in brain tissue. Meanwhile, it showed advances LDH activity(P<0.01) and IL-6(P<0.001). IL-1β(P<0.01) and TNF-α(P<0.05) levels in serum. After treated with Gen, the rats with cerebral I/R injury showed decreases in the volumes of infarction, water content (P<0.05), LDH activity (P<0.05), and IL-6(P<0.001), IL-1β (P<0.05) and TNF-α (P<0.01) levels in serum, and an increased in LDH activity in brain tissue (P<0.05)). Conclusion: Genistein has a therapeutic effect on rat with cerebral I/R injury because its down-regulating inflammatory cytokines levels such as IL-1β,TNF-α,IL-6, suppresses inflammatory reaction, and then controls cerebral edema.关键词：genistein;cerebral ischemia/reperfusion injury;cerebral edema;cytokine
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Ziyin Qingre decoction on golden hamster in serum sex hormones and adnrogen receptor. Method: The animals were randomly divided into 4 groups,namely blank control group, positive control group, high and low dosage Ziyin Qingre decoction. Rats were respectively fed with saline solution, antisterone(10 mg·kg-1), Ziyin Qingre decoction(18,9 g·kg-1) for 4 weeks. The serum testosterone(T) and estradiol(E2) levels were detected using enzyme-linked immunosorbant assay(ELISA) method. The gene androgen receptor(AR) mRNA in flank organ was measured by real-time PCR. Result: Compared with blank control group, high and low dosage Ziyin Qingre decoction could reduce serum T level and serum E2 level(P<0.05).There was a difference between four groups in expressions of AR mRNA(P<0.05). The AR mRNA in high dosage group was decreased(P<0.05), compared to the blank control group. Conclusion: Ziyin Qingre decoction can increase the serum E2 level and suppress the expression of AR mRNA. These may affect the proliferation of sebaceous gland.关键词：Ziyin Qingre decoction;golden hamster;sex hormone;androgen receptor
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发布时间：2024-01-04 - 摘要：Objective: To determine the antioxidant activity of the total flavonoid aglycones (TFA) and its main component pinostrobin chalcone (PSC) separated from the leaves of Caryr cathayensis (LCC). Method: Polyamide column chromatography was used to extract TFA and PSC;the antioxidant activity of TFA and PSC against 2,2-diphenyl-l-picry lhydrazyl(DPPH), ferric reducing antioxident power(FRAP), lipid peroxide(LPO) and protein carbonylation(PCO) was assayed. Result: Total phenol content of TFA was equivalent(mg·g-1 extract) to 283.75 mg(gallic acid equivalent)·g-1. The scavenging of DPPH of TFA and PSC are (20.05±1.74), (11.66±0.61) mg·L-1.The inhibiting of LPO of TFA and PSC are (74.62±5.39), (24.38±1.24) mg·L-1. The inhibiting of PCO of TFA and PSC are(174.29±11.26), (86.74±7.89) mg·L-1. The antioxidant assay indicated that both TFA and PSC had a strong antioxidant activity, and except the FRAP activity, all other assay results manifested that the antioxidant activity of PSC was significantly stronger than that of TFA. Conclusion: The leaves of C. cathayensis with high amount of TFA, which with great antioxidant activity, and the compound PSC was the main active component, which was worthy to be further exploited.关键词：Carya cathayensis;total flavonoid aglycones;pinostrobin chalcone;total phenol;antioxidation
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antitumor effect and the mechanism of Atractylodes macrocephala essential oils on human lung cancer A549 cells. Method: A. macrocephala oil emulsion was orally given to rats, after 10 days collection containing drug serum was collected,A549 cells were cultured in serum,MTT method was used to measure the activity of A549 cells, and ELISA method was used to assay the expression of caspase-3 in A549 cells. Result: 150, 300 μL·kg-1 of containing A. macrocephalae oil 15% serum could significantly inhibit the proliferation of A549 cell,A549 cell cycle arrested in G0/G1 phase,and increased the content of caspase-3 protein in A549 cells. Conclusion: A.macrocephalae oil can inhibit the proliferation of A549 cells, its antitumor mechanism may control A549 cell cycle arrest in the G0/G1 phase, promote the expression of the caspase-3 protein.
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Tonglian decoction (TD) of morphology, the growth cycle and nuclear factor-kappa B (NF-κB) signaling pathway, and to discuss the inhibiting mechanisms on gastric cancer cell proliferation in gastric carcinoma cell line MGC803. Method: MGC803 cell line was seeded in 96-well micro-plate with 1×104 cells in each well, and cultured with DMEM containing 5% fetal bovine serum (FBS), and maintained in the incubator at 37 ℃ with 5% CO2 in humidified atmosphere. After cell adherence for 24 h, TD of different concentrations was administrated from 25-800 mg·L-1 with common ration of two, while Fluorouracil from 10-160 mg·L-1 as positive control group. 48 hours' administration was followed by observation on cell morphology by inverted microscope, and cell proliferation determined by MTT assay. The half maximum inhibition rates (IC50) of TD and fluorouracil were calculated by curve fitting method. After 48 hours' administration of TD and Fluorouracil at 48 h IC50 concentration, flow cytometry (FCM) was employed for cell cycle determination, and western blot for protien expression of inhibitor kappa B kinase β (IKKβ), NF-κB and phosph-NF-κB. Result: 48 h IC50 of TD is 192.74 mg·L-1, and that of Fluorouracil is 23.61 mg·L-1. Compared with control group, TD can significantly affect G2 phase of cell cycle in cell line MGC803, and Fluorouracil G1 phase (P<0.05);TD and fluorouracil could significantly decrease protein expression of IKKβ and NF-κB. Conclusion: TD has inhibition effect on cell line MGC803, which is related to inhibiting cells in G2 phase, and down-regulating protein expression of IKKβ and NF-κB.关键词：gastric carcinoma;cell line MGC803;Tonglian decoction;fluorouracil;cell cycle;IKKβ;NF-κB
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发布时间：2024-01-04 - 摘要：Objective: To study the preliminary antioxidant activities of ethanolextracts from several Chinese herbal medicine. Method: The in vitro scavenging activities of ethanol extracts by ultrasound on superoxide radical,hydroxyl radical and DPPH·were investigated with vitamin C(VC) as the positive control in this study. Result: The extracts of Glycyrrhiza uralensis, Salvia miltiorrhiza, Pinus massoniana, Angelica sinensis, Podocarpus macrophyllus had scavenging activities on superoxide radical(equivalent of raw material 100 g·L-1,the eliminate rate was 15.86%,11.68%,11.31%,7.88%,5.94%,respectively), DPPH·(IC50 value was 13.91,1.6, 1.87, 22.32, 3.11 g·L-1, respectively),·OH(IC50 value was 2.77, 1.6, 2.1, 4.03, 2.02 g·L-1, respectively). The VC IC50 value of the three experiments was 0.047, 0.93, 0.21 g·L-1. Conclusion: Five kinds of ethanol ultrasonic extract from Chinese herbal medicine have antioxidant activity, especially S. miltiorrhiza and P. massoniana, which havs the value of exploiting and utilizing.关键词：Pinus massoniana;Salvia miltiorrhiza;antioxidant activity;radical;in vitro
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发布时间：2024-01-04 - 摘要：Objective: To study the inhibitory effect of Yulangsan polysaccharide (YLSP) on duck hepatitis B virus (DHBV) persistent infection in the model for the serum DHBV-DNA and the protecting effect on hepatocytes. Method: Ducklings were infected with DHBV and randomly divided the 3YLSPS dose groups(10, 5, 2.5 g·kg-1), lamivudine (3TC, 0.05 g·kg-1) and model groups respectively,continuous administration for 14 days. The serum content of DHBV-DNA was determined by real time fluorescent quantity polymerase chain reaction(FQ-PCR) method, DHBsAg and DHBeAg in serum were detected by ELISA before treatment and on the 7th, 14th day after treatment and At 3 days after stopping treatment, took the liver homogenate and detected in liver and serum superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA), L-glutathione (GSH) content. Result: Compared with modle group, the serum levels of DHBV-DNA, DHBsAg and DHBeAg decreased significantly 7 and 14 days after treatment with high-dose and middle-dose YLSP and at 3 days after stopping treatment the DHBV-DNA, DHBsAg and DHBeAg in serum in high-dose group were not rebounded, in liver and serum SOD, GSH-Px activity and MDA, GSH content, high-dose and middle-dose YLSP could still show a sustained and effective, no rebound phenomena (P < 0.05 or P < 0.01). Conclusion: YLSP can inhibit replication of DHBV and protect hepatocytes.
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发布时间：2024-01-04 - 摘要：Objective: To study effect of portalaca total flavone(PTF)on H9c2 myocardial cell inj induced by anoxia/reoxygenation (A/R)and its possible mec hanism. Method: Myocytes cell model of A/R injury was establis hed in vitro cultured.The cell viability was measured with MTT,Intra cellular calcium concentration was determined by Fluorospectrop hotometry,nitric oxide(NO) concentration were detected using colori metricmet hod,using flow cytometry to detect myocardial cell apoptosis rate, myocardial cell apoptosis gene expression of Caspase-3 mRNA detected by RT-PCR. Result: Compared with the A/R group,after pretreatment with 10,20 and 40 mg·L-1 PTF,PTF groups can significantly improve anoxia/reoxygenation injury of myocardial cell survival rate, reduce the concentration of NO, intracellular calcium ion concentration, the cell apoptosis rate (P<0.05, P<0.01), PTF groups can effectively reduce hypoxia/reoxygenation injury of myocardial Caspase-3 mRNA expression in the cell. Conclusion: PTF can protect the myocardial cells with in hibit myocardial intracellular calcium overload, reducing excessive NO cytotoxic effect and downgrading of caspase 3 mRNA expression.关键词：portalaca total flavone(PTF);Myocardial cell;anoxia/reoxygenation(A/R);apoptosis;Ca2;NO;Caspase-3
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect on lung adenocarcinoma transplantation tumor in nude mice and the influence of phosphatidylinositol 3 kinase/protein kinase B(PI3K/AKT)signaling pathway of Buzhong Yiqi decoction. Method: 50 nude mice were randomly divided into 5 groups, intervented respectively after 14 days, put to death and calculating tumor inhibition rate and used immunohistochemical method and real time PCR(RT-PCR) detecting the expression level of PI3K,AKT in transplantation tumor tissue. Result: Conbined group was the strongest inhibition rate of transplanted tumor, compared with other groups,there are statistically significant (P<0.05). The q value of synergistic effect of cisplatin and Buzhong Yiqi decoction was 1.201. PI3K and AKT protein and gene mRNA expression in conbined group were decreased. There are statistically difference among other groups (P<0.05). Conclution: The Buzhong Yiqi decoction combined with cisplatin has synergistic inhibitory effect in Cisplatin resistance tumor.The Buzhong Yiqi decoction can increase cisplatin resistance mechanism throught regulating the gene and protein expression of PI3K and AKT.关键词：Buzhong Yiqi Decoction;lung adenocarcinoma;transplantation tumor;PI3K;Akt
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发布时间：2024-01-04 - 摘要：Objective: To observe eye irritation, and therapeutic effect of Shuangqin ophthalmic gel on experimental rabbit herpes simplex keratitis (HSK), as well as evaluate the ocular residence time of Shuangqin ophthalmic gel. Method: The experimental HSK was induced by inoculation of HSV-1 (SM44 strain) on the scratched cornea. Figty rabbits with HSK were divided into 5 groups:10% Shuangqin group, 20% Shuangqin group, 30% Shuangqin group, acyclovir eye drop group, and gel substrate group. Acyclovir eye drop group was treated 5 times a day, while the other groups were treated 4 times a day. The severity of corneal epithelial lesion and stromal lesion were graded and the mortality rates of the different groups were recorded. Eye irritation of the Shuangqin ophthalmic gel with the best therapeutic effect was evaluated by irritation experiment, and the retention time of Shuangqin ophthalmic gel was measured by fluorescence tracer method. Result: On the 7th day after treatment, ACV eye drop and Shuangqin ophthalmic gel had a better therapeutic effect on both epithelial HSK and stromal HSK than other groups, the severity scores in the five groups were significantly different (P<0.05). There was no significant difference between 30% Shuangqin ophthalmic gel group and ACV group. On the 14th day after treatment, the mortality rate was lowest in the Shuangqin ophthalmic gel group, but there was no significant difference among the groups. The result of irritation test showed 30% Shuangqin ophthalmic gel both had no eye irritation after treatment for one hour and 7 days. The irritation scores in the Shuangqin ophthalmic gel group, ACV group and physiological saline group were not significantly different. The residence time of Shuangqin ophthalmic gel was (133±22.5) min and (54.13±8.48)min in the conjunctival sac and cornea respectively, there was significant difference compared with ACV group and physiological saline group(P<0.05). Conclusion: 30% Shaungqin ophthalmic gel has a good therapeutic effect on experimental HSK, a prolonged residence time and no eye irritation, therefore it is a very good new drug candidate for HSK.关键词：Shuangqin ophthalmic gel;herpes simplex keratitis;eye irritation;ocular residence time
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the toxicity of imcompatible pair of Sargassum and Radix Glycyrrhizae in mice by comparing the acute toxicity of different Haizao Yuhu decoction groups. Method: The mice was given different combination and compatibility of Haizao Yuhu decoction with different doses by intragastric administration, and calculated the LD50. If the group didn't meet the LD50 evaluation criteria we would give the drug by continuous intragastic administration for 28 days, ie, we would evaluate the toxity by caculating cumulative median lethal dose. The blood of mice was collected by picking bulb of eye. Detected relevant indicators of liver and kidney function in mice serum to evaluate their influence on liver and kidney. Result: The LD50(n) of the Haizao Yuhu decoction(Radix Glycyrrhizae) is 71.08 g·kg-1,the LD50(n) of the Haizao Yuhu decoction (the prepared Radix Glycyrrhizae) is 68.28 g·kg-1,the LD50(n) of the Haizao Yuhu decoction removed Radix Glycyrrhizae is 55.78 g·kg-1,the LD50(n) of the Haizao Yuhu decoction removed Sargassum(Radix Glycyrrhizae) is 72.27 g·kg-1,the LD50(n) of the Haizao Yuhu decoction removed Sargassum and Radix Glycyrrhizae is 79.62 g·kg-1. The Haizao Yuhu decoction removed Sargassum (the prepared Radix Glycyrrhizae) still didn't reach median lethal dose after continuous administration for 28 days, the maximum tolerated dose of this group is 80 g·kg-1;each experimental groups were not showed a significant impact on the liver and kidney functions in mice. Conclusion: The LD50(n) of each experimental group belongs to non-toxic category, and each group has no significant impact on the liver and kidney system in mice.关键词：Sargassum;Radix Glycyrrhizae;imcompatible pair;Haizao Yuhu decoction;acute toxicity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of compatibility of Astragalus and different dosage of Radix Bupleuri on the model of spleen-qi deficiency with fever in rats. Method: The male Wistar rats were assigned into the normal control group modle group, the spleen-qi deficiency with fever group (model group), the Astragalus with high dosage Radix Bupleuri group, the Astragalus with medium dosage Radix Bupleuri group, the Astragalus with low dosage Radix Bupleuri group, and the only Radix Bupleuri group. The rats in model group and medicine groups were treated with excess fatigue plus out of constant diet for 21 days. From the 17th day, the medicine group rats were fed with 4.56, 2.95,2.41 g·kg-1 and 2.41 g·kg-1 (Radix Bupleuri only) once a day and the other two groups were fed with physiological saline. On the 22th day,the rats in model group and medicine groups were respectively intraperitoneally injected with lipopolysaccharide(LPS,80 μg·kg-1) to induce fever.The rats of the normal group were injected with physiological saline.The appearance and behavior characteristics of rats were observed twice a week and the rectal temperature was measured at different time after ip LPS. The content of braingut petide in peripheral blood was also measured. Result: Compared with the normal group,the rectal temperature of the spleen-qi deficiency with fever group raised at each time point in different degree after ip LPS, especially at 60, 220 min(P<0.05);the integration of appearance and behavior characteristics increased significantly(P<0.05);somatostatin(SS), cholecystokinin-8(CCK-8), β-endomorphin(β-EP) in peripheral blood increased significantly(P<0.05). Compared with model group, the rectal temperature of every medicine group were decreased significantly in different degree(P<0.05), especially in the only Radix Bupleuri group and the Astragalus with low dosage Radix Bupleuri group, the temperature curve deviated from the normal group;the integration of appearance and behavior characteristics decreased significantly(P<0.05)only in the Astragalus with medium and low dosage Radix Bupleuri groups;serum β-EP increased significantly(P<0.05)in all medicine groups;serum CCK-8, SS showed a downward trend in the Astragalus with high, medium and low dosage Radix Bupleuri groups, but sexum CCK-8, SS in the only Radix Bupleuri group showed a rising trend. Compared with the only Radix Bupleuri group, serum SS decreased significantly(P<0.05) in the Astragalus with high, medium and low dosage Radix Bupleuri groups;serum β-EP decreased significantly(P<0.05) in the Astragalus with large dosage Radix Bupleuri group;serum CCK-8 decreased significantly(P<0.05)in the Astragalus with medium and low dosage Radix Bupleuri groups. Conclusion: The compatibility of Astragalus and different dosage of Radix Bupleuri has antipyretic effect on the model group and the integration of appearance and behavior characteristics and braingutpetide were improved in different degrees. The only Radix Bupleuri group has the antipyretic effect only. The Astragalus with low dosage Radix Bupleuri group has the best controlling effect for the comprehensive indices.关键词：Astragalus Mongholicus;Radix Bupleuri;compatibility;the spleen-qi deficiency with fever
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of mango leaf extract (MLE) on acute alcoholic liver injury in mice. Method: Sixty mice were randomly divided into control group, model group, positive drug group(Hugan tablet 1.5 g·kg-1), MLE high, middle and low-dose(15.0, 5.0, 2.5 g·kg-1)group, 10 each group. Acute liver injury model was induced by 50% alcohol given by gavage. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), triglycerides(TG) in the serum and malondialdehyde(MDA), glutathione(GSH), total superoxide dismutase (T-SOD) in the liver tissue were measured. Liver index was calculated and pathological changes of the liver tissue were observed under optical microscope. Result: Compared with model group,the serum TG in MLE high, middle and low dose group significantly lowered (P<0.05);the serum ALT, AST in MLE high, middle dose group significantly lowered (P<0.05);T-SOD activity in MLE high dose group rose obviously (P<0.05);GSH levels of liver tissue in MLE high and middle dose group rose obviously (P<0.05);MDA content in liver tissue in MLE high, middle and low dose group lowered obviously (P<0.05);the liver pathological score of MLE high, middle and low dose group lowered significantly (P<0.05). Conclusion: MLE has a protective effect on acute liver injury induced by alcohol in mice,which may be related to its anti-lipid peroxidation.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of different extracts of Cydonia oblonga Mill. (COM) fruits and leaves on blood pressure and hemorheology in renal hypertensive rats(RHR). Method: Two-kidney,one-clip (2K1C) rat model was established and divided randomly into eleven groups:sham group, model group, low and high dosage of aqueous extracts of COM fruits groups, low and high dosage of ethanol extracts of COM fruits groups, low and high dosage of aqueous extracts of COM leaves groups low and high dosage of ethanol extracts of COM leaves groups(80,160 mg·kg-1) and captopril group (25 mg·kg-1)(n=10, each). The drugs were administered for eight weeks;systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured before administration and every 2 weeks, the changes of hemorheology perameter were examined after the last administration. Result: After treatment for eight weeks, in all groups of COM fruits and leaves extracts the SBP and DBP of RHR were lowered in different degrees(P<0.05), the whole blood viscosity, plasma viscosity, hematocrit, erythrocyte aggregation index and erythrocyte rigidity index were declined(P<0.05), the erythrocyte deformability index was elevated(P<0.05). The effects of ethanol extracts groups were better. Conclusion: COM extracts have antihypertensive activity and certain influence on hemorheology perameter, and the active ingredients are higher in ethanol extracts.关键词：Cydonia oblonga;renal hypertensive rats;systolic blood pressure;diastolic blood pressure;hemorheology
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发布时间：2024-01-04 - 摘要：Objective: Through researching the effection of Naohuan Dan(NHD) on behavior and the expression of β-amyloid precursor protein (APP), peptidyl- prolyl cis-trans isomerase A (Pin1) and high mobility group protein Bl (HMGB1) mRNA in the hippocampus of Senescence Accelerrated Mouse SAMP/8, this work discusses partially the mechanism of traditional Chinese medicine NHD with respect to its effectiveness to treat Alzheimer's disease. Method: Sixty six-months old SAMP/8 mice were randomly divided into NHD low and high dose group and model group, 10 six-months old SAMR/1 were served as a normal control group. The mice were administered with NHD intragastricly at the dose of 84, 21 g·kg-1 per day for 8 weeks respectively, while distilled water in the model group and normal control group at the dose of 10 mL·kg-1.8 each analyzed the parameter of place navigationtest and spatial probe test by Morris water maze,then stripped out whole brain under sterile condition,the hippocampus were taken out to expression of APP, Pin1, HMGB1 mRNA by real time quantitative PCR (qRT-PCR). Result: 6 month old male SAMP/8 learning and memory ability was significantly lower than the same month-old SAMR/1 male, specific performance: the escape latency was significantly increased from the second day,the time of stopping on original platform quadrant was shortened. The SAMP/8's escape latency was significantly shortened(P<0.05). The memory retention in SAMR/1 mice and mice after NHD treatment was stronger than SAMP/8, especially the high dose NHD group was obvious(P<0.05).The relative expression of APP,Pin1,HMGB1 mRNA was statistical significant difference.In the model group,the level of APP mRNA increased compared with the normal control group(P<0.05); the level of APP mRNA of NHD group decreased compared with the model group(P<0.05). In the model group,the level of Pin1 mRNA decreased compared with the normal control group(P<0.05); the level of Pin1 mRNA of NHD group increased compared with the model group(P<0.05); In the model group,the level of HMGB1 mRNA increased compared with the normal control group(P<0.05); the level of HMGB1 mRNA of NHD group decreased compared with the model group(P<0.05);The effects in the NHD high dose group were all better than those in the low dose group for APP mRNA, Pin1 mRNA and HMGB1 mRNA. Conclusion: Naohua Dan treat SAMP/8 of the more recognized animal model of AD for 8 weeks.Behavior detection was found that Naohuan Dan can improve the learning ability,memory impairment of the SAMP/8.The increased level of APP and HMGB1 mRNA and the decreased level of Pin1 mRNA in the SAMP/8 mice,which probably is part of pathology of Alzheimer's disease. Naohuan Dan can block amyloid plaques and nerve fibers tangles from source through decreacing the APP and HMGB1 level and increscing the Pin1 level.This may be one of the major action points of the treatment of AD by Nanhuan Dan.
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Scutellaria barbata polysaccharide on the fluidity of erythrocyte membrane and its mechanism of red cell immune regulation. Method: Fourty eight mice were randomly divided into six groups:normal control group, model control group, Astragalus polysaccharide group (100 mg·kg-1), S. barbata polysaccharide Dou low, middle, and high dose group (50,100,200 mg·kg-1).The membrane fluidity of erythrocytes in tumor-bearing mice was determined by fluorescence spectrophotometry;the erythrocyte membrane Ca2+, Mg2+-ATPase activity sialic acid (SA) content, antioxidant enzymes activity were detected by UV spectrophotometer. Result: Compared with the modle group, S. barbata polysaccharide low dose, middle dose, high group could obviously improve the fluidity of erythrocyte membrane in S180 tumor-bearing mice(P<0.01, P<0.05), obviously increase the red cell membrane Ca2+, Mg2+-ATPase activity (P<0.01, P<0.05) and antioxidant enzymes activity(P<0.01, P<0.05); low, middle group increased sialic acid content of red cell membrane surface (P<0.01). Conclusion: S. barbata polysaccharide can increase the fluidity of erythrocyte membrane and enhance the immune function of erythrocyte in S180 tumor-bearing mice, thus it may exert aiti-tumor effect by improving the function of erythrocyte membrane.关键词：Scutellaria barbata polysaccharides;anti-tumor;fluidity of erythrocyte membrane;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To explore mechanism of the liver injury caused by giving Eudiae Fructus evodiae decoction in mice. Method: KM mice were divided into four groups:normal control,high,middle and low dose groups which were administrated respectively with distilled water or equal volume of Euodiae Fructus decoction at the doses of crude drug 30,20,10 g·kg-1 daily by gavage for consecutive 21 days. The blood of the animals was collected by pulling out the eyeball. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automatic biochemical analyzer. The livers were collected. The liver weight indexes were calculated and liver pathological changes were observed. The activity of superoxide dismutase (SOD),glutathione (GSH),glutathione peroxidase (GSH-Px),nitric oxide synthase (NOS)and the contents of malonaldehyde (MDA)in liver samples were measured by Kit. The ratio of SOD/MDA was calculated. The contents of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α) in liver samples were detected by ELISA. Result: Compared with control group,the activities of ALT and AST were significantly increased in high,middle and low dose groups. The liver histopathological change was characterized by emergence of focal necrosis of liver cells. The activity of GSH-Px,NOS and the ratio of SOD/MDA in the liver were significantly decreased (P<0.05), the tissue content of GSH was increased (P<0.05), the contents of IL-1β,IL-6,TNF-α of the liver were significantly increased(P<0.01). Conclusion: The mechanism of the liver injury caused by high dose of Euodiae Fructus decoction in mice may be related to oxidative stress and inflammatory response.关键词：Euodiae Fructus;mechanism of hepatotoxicity;free radicals;inflammatory factors
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发布时间：2024-01-04 - 摘要：Objective: This study tries to approach the long-term toxicity of Radix Aconiti (RA) combined with Fructus Trichosanthis (FT) in rats, and investigate the relationship between compatibility and toxicity about this anti-drugs. Method: Eighty male and female SD rats were randomly divided into RA 0.3 g·kg-1, RATF 0.3, 0.1 g·kg-1 and the contrast groups, each group having 10 male rats and 10 female rats. the rats of the drug administered groups received drugs orally with the water extract of RA and RAFT,the oral volume was 5 mL·kg-1,the rats were administered one time a day for 30 days. 24 hours later after the last time of drug administration, 2/3 of the rats were sampled blood from their abdominal aorta and organs to figure the indexes of organs out, detecting heart creatine kinase(CK), lactate dehydrogenase(LDH), liver aspartate aminotransferase(AST), alanine aminotransferase(ALT), kidney blood ureanitrogen(BUN), creatinine(Cr) and other biochemical markers, making the pathological examination for Major tissues and organs. The remaining 1/3 rats were detected the corresponding indicator in the same way 15 days later after stopping drug administration. Result: The kidney index of female rats in every drug administered groups was higher than contrast group's and the thymus index was lower. The RBC were higher than the contrast group (P<0.05). The AST, ALT, LDH and BUN of each drug administered group were higher than the contrast group (P<0.05,P<0.01), and the Cr of female rats was higher than the contrast group in RA 0.3 g·kg-1 and RAFT 0.3 g·kg-1 groups(P<0.05,P<0.01). The AST,LDH of female rats in RA 0.3 g·kg-1 group were higher than ARTF 0.1 g·kg-1 group (P<0.05,P<0.01). The AST, ALT, Cr, LDH of female rats and the AST of male rats in RAFT 0.3 g·kg-1 group were higher than RAFT 0.1 g·kg-1 group (P<0.05,P<0.01). A certain extent visceral injury can be seen in heart, liver, and kidney of the rats in RAFT 0.3 g·kg-1 group through main viscera conventional HE dying. The AST,ALT of female rats and the ALT of male rats in RA 0.3 g·kg-1 and RAFT 0.3 g·kg-1 group were higher than contrast group at the end of recovery term. The differences of AST,ALT in AR group and ARTF group were not statistically significant. Conclusion: Radix Aconiti has significant toxicity in heart, liver and kidney of rats in the given dose. The long-term toxicity has no obvious difference between RA group and RAFT group in rats, but more harmful in female rats than in male.关键词：Radix Aconiti;Fructus Trichosanthis;compatibility;long-term toxicity
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to observe the influence of Xiefei Ningxin decoction on vascular endothelial function and inflammatory factor in acute stage of chronic cor pulmonary heart disease (CPHD). Method: Ninety-six cases of CPHD patients were randomly divided into control group (48 cases) and observation group (48 cases) according to digital method. The patients in both groups took supportive treatment, aiming at anti-infection, relieving cough and asthma, eliminating phlegm, diuresis, heart failure, etc. The patients in observation group took Xiefei Ningxin soup, 1 dose/day, course treatment 14 days. The blood gas indexes, such as Arterial carbon dioxide partial pressure (PaCO2), oxygen partial pressure (PaO2) and the blood oxygen saturation (SaO2) were monitored. Pulmonary artery pressure (mPAP) and Tei index were measured, detecting plasma endothelin 1 (ET-1), nitric oxide (NO), tumor necrosis factor α(TNF-α) and interleukin 8 (IL-8) level. The cardinal symptom and physical sign score were recorded. Result: The total curative effect of the observation group was 93.75%, superior to 77.08% of the control group (P<0.05). After treatment, PaO2 and SaO2 of patients in observation group were higher than the control group, while PaCO2 is lower than the control group (P<0.01). The mPAP, myocardial performance index, cardinal symptom and physical sign score were lower than control group (P<0.01).ET-1 of observation group was lower than the control group, while the NO level was higher than the control group (P<0.01). The levels of TNF-α and IL-8 of observation group were lower than the control group (P<0.01). Conclusion: Xiefei Ningxin decoction can improve clinical symptoms and physical signs of CPHD patients in acute stage, improving ventilation/air change function and heart function and reducing mPAP. The mechanism may be related to regulating endothelial function, reducing the inflammatory reaction.关键词：chronic pulmonary heart disease;acute phase;Xiefei Ningxin decoction;pulmonary artery pressure;myocardial performance index;endothelial function;inflammatory cytokines
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发布时间：2024-01-04 - 摘要：Objective: To evaluate control of Gushen Dingchuan Pill on pulmonary function and quality of life in patients of stable stage of chronic obstructive pulmonary disease (COPD). Method: Ninety-four cases of stable stage of COPD with qi deficiency of lung and kidney were randomly allocated into control and experimental groups, 47 cases each group. Both groups were treated according to the guideline of COPD diagnosis and therapy. Gushen Dingchuan pills were added into the experimental groups, 2 g/time, three times/day. The treatment courses lasted for 12 weeks. Lung function (LF), respiratory muscle fatigue indicators (MIP), The st george's respiratory questionnaire(SGOR) measuring quality of life, with the index(BODE) on the price of disease severity, record before and after lung and kidney qi deficiency treatment score. Result: Forced expiratory volume in one second,forced expiratory volumeinone second(FEV1);forced vital capacity(FVC),modified Medical research council scale(MMRC);6 minutes walk distance(6MWD);bodynlass index, airflow obstruction, dyspnea, and exercise capacity index,BODE respiratory muscle fatigue indicators (MIP) increased in experimental group while those decreased in control, showing the experimental better than the control(P<0.05 or P<0.01);3 dimensions including respiratory symptoms, activity and disease impact and the overall scores of SGOR of experimental group were significantly lower than the control (P<0.05);FEV1,mMRC,6 MWD and BODE scores of experimental group were lower than those in control(P<0.05 or P<0.01);overall effectiveness of the experimental with 89.36% was better than the control with 57.44% (P<0.01). Conclusion: Gushen Dingchuan pill improved LF, respiratory muscle recovery, clinical symptoms, activity and quality of life of stable stage of COPD with qi deficiency of lung and kidney.关键词：chronic obstructive pulmonary disease;Gushen Dingchuan pill;lung function;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Self-made Qiangshen Yugan decoction combined with entecavir on treating chronic hepatitis B(CHB) and the influence on(IL-12),(IL-21). Method: Eighty patients with chronic pyelonephritis were randomly divided into observation group (n=40) and control group (n=40). Patients in the two groups were both treated with entecavir. Self-made Qiangshen Yugan decoction was used in the observation group (one dose per times, 24 weeks as one cycle). Levels of serum hepatitis B virus-DNA(HBV-DNA),Hepatitis B virus markers(HBV-M),interleukin-12(IL-12) and interleukin-21(IL-21) were tested, liver function and clinical symptoms and signs were also observed. Result: Response rate of observation group (85.0%) was better than the control group (55.0%)(P<0.05);after treatment, HBV-DNA copy number of the observation group was significantly decreased, and lower than the control group (P<0.05). HBV-DNA negative conversion rate of observation was 82.5%, higher than the control group(52.5%)(P<0.05);HBeAg negative rate of observation group was 42.5%, higher than the the control group(20.5%) (P<0.05);aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyl transpeptidase (GGT) and total bilirubin(TBil) levels of observation group were low than control group (P<0.01), ALT normalization normalization rate of observation group was 85.0%, higher than the control group(52.5%) (P<0.05);Serum IL-12, IL-21 levels of observation weeks treatment, group were significantly increased and higher than the control group (P<0.05,P<0.01);after 8 weeks treatment, symptom scores of observation group were significantly decreased, at a low level, and was lower than the control group (P<0.01). Conclusion: Self-made Qiangshen Yugan decoction can inhibit HBV replication, reduce HBeAg expression, it also can improve clinical symptoms and signs, promote the recovery of liver function. Its mechanism may be able to increase CHB patients IL-12, IL-21 levels, immune function and it is worth further study.关键词：chronic hepatitis B;Self-made Qiangshen Yugan decoction;entecavir;interleukin-12;interleukin-21
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to study of Xintong Zhuyu decoction on platelet function of unstable angina (UA) patients and the improvement of vascular endothelial function. Method: Seventy-eight cases of UA patients were randomly divided into observation group (39 cases) and control group (39 cases) according to the hospital order. The patients in both groups took general treatment. The patients in control group took clopidogrel tablets, 75 mg/time, 1 time/day, orally. The patients in observation group took Xintong Zhuyu decoction, 0.5 dose/time, 2 times/day, conventional water frying. The course treatment for both groups is 8 weeks. Platelet aggregation rate (MPAR),the plasma platelet α-membrane particle protein (GMP-140), thromboxane B2(TXB2), plasma endothelin (ET) levels and hemodynamic indexes will be test before and after the treatment. Result: The total curative effect of observation group was 92.3%, which is higher than 73.52% of the control group (P<0.05). The total curative effect of electrocardiogram of observation group was 82.1%, superior to 61.5% of the control group (P<0.05).After the treatment, the levels of MPAR, GMP-140, TXB2 and ET of observation group were all lower than control group (P<0.01). The improvement of blood rheology indicators, including whole blood viscosity (high shear, low shear), plasma viscosity, fibrinogen and red cell aggregation index, were better than control group (P<0.01). Conclusion: Xintong Zhuyu decoction can improve platelet aggregation rate, inhibit the formation of blood clots, improve microcirculation status and endothelial function and improve the curative effect and electrocardiogram curative effect. So it is worth promoting in clinical treatment.关键词：unstable angina;Xintong Zhuyu decoction;platelet aggregation rate;plasma platelet α-membrane particle protein;thromboxane B2;plasma endothelin
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发布时间：2024-01-04 - 摘要：Objective: The discussed is to study the control of Naoxintong capsule on adiponectin and endothelial function of coronary heart disease patients with hyperlipidemia. Method: Eighty cases of patients were randomly divided into control group (40 cases) and observation group (40 cases) by digital method. Patients in two groups all took conventional western medicine treatment, atorvastatin, 20 mg/time, before sleeping at night orally. In addition, patients in observation group took Naoxintong capsule, 1.6 g/time, 3 times/day. The courses were all 12 weeks. serum adiponectin (APN), von Willebrand factor(vWF), serum nitric oxide (NO), endothelin 1(ET-1), thromboxane(TXB2) and 6-ketone-prostaglandin F-(1a)(6-Keto-PGF-1a) were tested before and after treatment, as well as blood lipid level and the examination of the electrocardiogram. Result: The total effective rate of observation group was 95.0%, higher than that of control group 80.0% (P<0.01). The total effective rate of the electrocardiogram effect of observation group was 80.0%, superior to 55.0% in the control group (P<0.01). The levels of cholesterol(TC) and triglycerides(TG) decreased significantly and were lower than the control group (P<0.01). After treatment, the serum APN levels of both groups increased, while obvious increasing occurred in observation group (P<0.01). Serum vWF and TXB2 of two groups decreased and observation group was lower than the control group (P<0.01). NO and 6-Keto-PGF-1a increased in both groups and observation group was higher than the control group(P<0.01).Obvious decreasing of ET-1 took place in both groups and observation group is lower than the control group (P<0.01). Conclusion: Naoxintong capsule can increase APN level of coronary heart disease patients with hyperlipidemia, inhibit inflammatory reaction, by improving the proportion of ET/NO and PGI2/TXA2, decreasing vWF level positive regulating endothelial function, and thus play a role of resistance to atherosclerosis.关键词：coronary heart disease;hyperlipidemia;Naoxintong capsule;adiponectin;endothelial function
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to study the curative effect of Yi Kang pill on Graves Disease (GD) and its influences on autoantibodies and adipocytokines. Method: Ninety-two cases of GD patients were randomly divided into control group (46 cases) and observation group (46 cases) according to digital method. Patients in the control group took methimazole, 10 mg/time, 3 times/day, adjusting the dosage according to the index of thyroid function. On the basis of treatment in the control group, patients in the observation group took Yikang pill, 7.5 g/time, 2 times/day. Treatment course was 12 weeks. The cardinal symptom and signs of integral change of before and after the treatment of 4, 8 and 12 weeks were recorded. Serum thyroid stimulating hormone (TSH), triiodothyronine (T3), serum thyroxine (T4), serum free triiodothyronine (FT3) and serum free thyroxine (FT4) were detected before and after the treatment. Levels of anti-thyroglobulin antibody (TGAb), thyroid microsomal antibody (TMAb) and thyroperoxidase antibodies (TPOAb) were detected before and after the treatment. Levels of serum free fatty acid (FFA), adiponectin (APN), leptin (LP) and resistin (ADSF) were detected before and after the treatment. Result: The total effect of the observation group was 95.6%, superior to 82.6% of the control group (P<0.05). The decline range of cardinal symptom and signs of integral change of observation group was of better than that of control group in eighth weeks and twelfth weeks after the treatment (P<0.01). After treatment, TSH level of observation group was higher than the control group, while levels of T3, T4, FT3 and FT4 were lower than the control group (P<0.05 or P<0.01). TGAb, TPOAb and TMAb of observation group were lower than the control group (P<0.01). Levels of FFA, APN and ASDF were lower than the control group (P<0.01), while the LP level higher than the control group (P<0.01). Conclusion: Yikang Pill can obviously improve clinical symptoms of GD patient, improving the clinical curative effect. Its mechanism may be related to decrease autoantibodies levels of GD patients and to adjust abnormal immune. Yikang Pill can also regulate adipocytokines level of GD patients, further improving abnormal glucolipid metabolism of GD patients.关键词：Graves disease;Yikang pill;thyroid autoantibodies;adipocytokines
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Wenxin granule in treating chronic pulmonary heart disease complicated by ventricular contractions. Method: This study in 2010-11-2012-11 November in accordance with the random number table method treated the chronic pulmonary heart disease complicated with recurrent patients were randomly divided into study group and control group of premature ventricular contraction, the research group was treated with Wenxin granule, and carries out a comparative research on the clinical efficacy and adverse reactions and the control group with routine comprehensive therapy the. Result: The study group was significantly better than the control group in the clinical curative effect and electrocard iograph(ECG) efficacy (P<0.05). Patients in the study group after treatment on hemorheology significantly higher than those in control group (P<0.05). After treatment, the study group ventricular extrasystoles times lower than that of control group (P<0.05). Conclusion: Wenxin granule in the treatment of chronic pulmonary heart disease complicated by ventricular contractions had curative effect, less adverse reaction, and easy to accept.关键词：Wenxin granule;chronic pulmonary heart disease;ventricular contractions
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of traditional Chinese medicine(TCM) combined with spectrum of biological treatment of closed injuries. Method: This study in 2012-01-2013-01 were closed injury were randomly divided into study group and control group, the study group were treated with TCM combined with spectrum of biological treatment, and control group only used TCM treatment of clinical efficacy. Result: And the control group (71.43%) total efficiency compared to the study group (92.86%) was significantly higher than the control group, and the difference was statistically significant (P<0.05). The incidence of adverse reaction of two groups of patients (10.71% vs 7.14%) had no significant difference. Conclusion: TCM combined with spectrum had good curative effect in treatment of closed sport injuries, and the method was simple and practical.The price is low, and it is worthy of clinical use.关键词：spectrum;treatment;closed sport injuries
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of homemade prescription on henoch-schonlein purpura nephritis (HSPN) and the correlation with immune-regulating mechanism. Method: The 60 cases HSPN were randomly grouped to control group and treatment group, each group for 30 cases. The control groups were only used tripterygium wilfordii glycosides tablet, and the treatment groups were cured together with homemade prescription, and the two groups treatment was 3 months.After 3 months treatment continuously, to observe and compare the urinary protein reversed,the urinary red blood cell reversed,the overall clinical effect and safety before and after treatment;and the level of interlenkin-6 (IL-6), IL-10, IL-18 and tumor necrosis factor-α(TNF-α) in serum before and after treatment were detected. Result: After 3 months' treatment continuously, the treatment group was better than the control group (only used tripterygium wilfordii glycosides tablet) on the urine protein reversed,the urinary red blood cell reversed, the overall clinical effect, safety, and so on, and there was significant difference(P<0.05 or P<0.01);and the treatment group was better than the control group on the drop degree of IL-6,IL-18 and TNF-α level in serum, as well as the elevated degree of IL-10 level. Conclusion: The clinical effect of homemade prescription on HSPN was positive, and there was no obvious side effects, and it was better than the control group, and it was worthy popularized and applied in pediatric clinic. Its clinical curative effect was closely correlated with the regulating body's immunity and improve body's internal environment.关键词：homemade prescription;henoch-schonlein purpura nephritis (HSPN);clinical efficacy;immune-regulating mechanism
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of integrated traditional Chinese and western medicine in treatment of elderly hypertension complicated with hyperlipemia patients. Method: One handred and twenty-eight elderly cases with hypertension complicated with hyperlipemia were randomly divided into control group and experience group. Control group were given routine western medicine treatment, and meanwhile experience group received extract oral. The course of treatment was 4 weeks. Antihypertensive effect and the blood rheology indexes were compared between two groups. Result: The effective rate of treatment in experience group was much higher than that in control group (92.4% vs 71.0%,P<0.05);systolic blood pressure(SBP) and diastolic blood pressure(DBP) in experience group were (126.76±10.41) mmHg and (78.13±5.21) mmHg respectively, of which were superior to control group (142.33±12.62) mmHg and (84.24±5.47) mmHg (P<0.05);comparison with control group[(1.97±0.20), (5.38±0.57) and (3.35±0.73) mmol·L-1], triglyceride(TG), total cholesterol(TC) and low-density lipoprotein(LDL-C) level in experience group greatly decreased[(1.68±0.16),(4.60±0.51), (2.94±0.62) mmol·L-1,P<0.05)];whole high blood viscosity, whole low blood viscosity and blood viscosity were[(3.68±0.74),(16.33±2.40),(1.30±0.43) mPa·s in experience group,and (5.26±1.17),(21.46±2.84),(1.68±0.55) mPa·s in control group(P<0.05). Conclusion: Integrated traditional Chinese and western medicine in treatment of elderly hypertension complicated with hyperlipemia patients have more advantages in antihypertensive and improving blood lipid.关键词：hypertension;hyperlipidemia;integrated traditional Chinese and western medicine
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发布时间：2024-01-04 - 摘要：Objective: To explore the clinical effect of traditional Chinese and western medicine for acute coronary syndrome and the influence for serum high-sensitivity C-reactive protein(hs-CRP) and interleukin-6(IL-6).Method: One hundred and eighteen cases with acute coronary syndrome were randomly divided into control group and experience group. Control group were given routine western medicine and experience group were administrated with traditional Chinese and western medicine. 40 g Huangqi injection and 20 mL Danshen injection were dissolved into 250 mL sodium chloride injection respectively, quiescent point, once a day. The course of treatment was 2 weeks.Result: After treatment, the effective rates was much higher than that in control group (91.7% vs 75.9%,P<0.05);the dose of nitroglycerin in experience group was (1.47±0.52) tablets/week, of which was much less than that in control group[(3.40±0.87) tablets/week, P<0.05)]. After treatment, tcm syndrome evaluation in two groups were both decreased, while the tcm syndrome evaluation in experience group was superior to control group[(0.90±0.26) vs (1.33±0.41)paper,P<0.05]. The serum triglyceride(TC),total cholesterol(TG), hs-CRP and IL-6 after therapy in experience group were (4.89±0.96),(1.60±0.52) mmol·L-1, (5.76±0.97) mg·L-1 and (76.59±14.38) ng·L-1,and of the above indexes in control group were (5.90±1.13), (1.84±0.67) mmol·L-1, (7.36±1.03) mg·L-1 and (102.85±19.88) ng·L-1 with significant difference between two groups(P<0.05).Conclusion: Traditional Chinese and western medicine for acute coronary syndrome greatly decrease the serum inflammatory factor and blood lipid, and improve the clinical effect.关键词：acute coronary syndrome;onflammatory factor;blood lipid;huangqi injection;Danshen injection
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发布时间：2024-01-04 - 摘要：Objective: To investigate the traditional Chinese medicine (TCM)constitution types of the patients with acute exacerbation of chronic obstructive pulmonary disease(COPD)with secondary pulmonary fungal infections (AECOPD), analyse the internal relativity, and discuss the ways and means of preventing and treating secondary pulmonary fungal infections, so as to provide basis for applying the TCM in preventing and treating secondary pulmonary fungal infections. Method: A retrospective study was performed in 365 AECOPD patients and their TCM constitution types were determined. Result: A total of 63 patients in 365 AECOPD patients were infected by secondary pulmonary fungi. Among the 63 cases, 21 cases(33.33%) were qi-deficiency type;15 cases(23.81%) were phlegm-wetness type;9 cases(14.29%) were yang-deciency type;7 cases(11.11%) were wet-heat type;5 cases(7.9%) were blood-stasis type;4 cases(6.35%) were yin-deciency type;2 cases(3.17%) were qi-depression type. Conclusion: The qi-deficiency type patients are more likely to be infected with secondary pulmonary fungi than other types' patients because of Zang and Fu Organ hypofunction.关键词：chronic obstructive pulmonary disease;epiphyte infection;constitution of TCM;the physique classification
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发布时间：2024-01-04 - 摘要：Objective: To investigate the links between the ‘blood arthralgia’ theory of Chinese medicine and Oxaliplatin-induced peripheral neurotoxicity,and the effectiveness and severity of Huangqi Guizhi Wuwu decoction on the effect for the treatment of Oxaliplatin-induced peripheral neurotoxicity. Method: Completely searching the clinical researches of Huangqi Guizhi Wuwu decoction for Oxaliplatin-induced peripheral neurotoxicity by computer from CBM,CNKI,Wanfang Data,VIP,Cochrane Library,Medcine,EMbase by the inclusive and exclusive criteria.RevMan 5.0 was used for Metaanalysis. Result: ①Based on the outcomes of 368 patients, 16 clinical trials were identified to meet the inclusion criteria. The meta-analyses showed statistically significant differences in favor of Huangqi Guizhi Wuwu decoction compared with mecobalamin for the effectiveness on the effect for the treatment of the peripheral neurotoxicity induced by Oxaliplatin,including the incidence and severity of peripheral neurotoxicity and SNCV,compared with the conventional therapy or mecobalamin.②For all researches the safety analysis found no serious adverse events or adverse reactions (ADR/AE). Conclusion: Huangqi Guizhi Wuwu decoction can prevent Oxaliplatin-induced peripheral neurotoxicity,reduce the incidence of severe peripheral neurotoxicity and alleviate the inhibition of SNCV of oxaliplatin.According to the clinical experimental research,there is no serious adverse events or adverse reactions (ADR/AE)of Huangqi Guizhi Wuwu decoction,so Huangqi Guizhi Wuwu decoction has a certain degree of security for clinical application.关键词：blood arthralgia;peripheral neurotoxicity;oxaliplatin;Huangqi Guizhi Wuwu decoction;Metaanalysis
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发布时间：2024-01-04 - 摘要：Objective: To analyze the composing principles of the 581 Preferred Ancient Herbal prescriptions collected in the book of'The Prescription of Traditional Chinese Medicine Dictionary' using traditional Chinese medicine(TCM) inheritance system. Method: Input traditional chinese medicine inheritance system,the composing principles were analyzed using the TCM inheritance system after data entry. Result: Based on the analysis on 581 preferred ancient herbal prescriptions,the frequency of each herb included in the databasewas computed and core combinations. Conclusion: The results of this study for future study and research provides reference.关键词：ancient herbal prescriptions;Preferred;TCM inheritance system;composition principle
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发布时间：2024-01-04 - 摘要：Objective: Analysis of Professor Li Fa-zhi in the treatment of AIDS drug laws of cough,to provide the corresponding drug reference basis for Chinese medicine treatment of AIDS cough. Method: By using complex network analysis method,research the AIDS patients with cough who accept professor Li Fazhi treated during the period of 2007 October to 2011 July that live in Henan Weishi County, analysis of pathogeny and pathogenesis of AIDS cough, syndrome differentiation of old Chinese medicine treatment and medication rule Result: Using a multidimensional query analysis,the research core of the treatment of AIDS cough drugs for licorice,Astragalus,Codonopsis Qi drugs,Coltsfoot flower, pinellia, wax gourd, Huatanzhike asthma medicine,Windproof, ephedra, Radix Angelicae dahuricae, diaphoretic drug;Core prescription for cold soup,Xiaoqinglong Decoction plus gypsum, Eriocaulon mixture and the modified Zhisou powder. Conclusion: Professor Li Fazhi treatment of AIDS ‘cough from lung and spleen deficiency’ argument,by Tonifying Qi and strengthening health,expelling wind and cold for the treatment of qi deficiency,syndrome of wind-cold attacking lung;If slightly yellow sputum or yellow discharge with Houttuynia cordata、dong guaren;if the drink in the outer cold syndrome with Xiaoqinglong Decoction plus gypsum addition and subtraction;if cough with itching of the throat with modified Zhisou powder;if the headache with Eriocaulon mixture;at the same time, do not forget to use phlegm and relieving cough and asthma drugs Such as almond, mulberry bark, semen Lepidii.关键词：AIDS cough;Chinese medicine;complex network analysis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effects and safety of tripterygium for ankylosing spondylitis(AS). Method: Such database as PUBMED, EMBASE-ASP,Cochrane Library,OVID,Springer-link CNKI, VIP, Wanfang and CBM, ect, were searched from the date of their establishment to December 2012.The quality of included studies was evaluated, and meta-analysis was conducted with RevMan 5.1 software. Result: A total of 8 RCTs involving 576 patients were included. Meta-analysis showed that there were no significant differences between the tripterygium and Chinese herbal compound groups in the effect on improving the chest movement, the Occiput-Wall distance,the Finger-Ground distance and C-reactive protein.The Chinese herbal compound showed more effects in improving the total effective rate, the duration of morning stiffness,the Schober test and reducing C-reactive protein.The tripterygium may cause to postpone to menstrual period,gastrointestinal adverse reactions,liver dysfunction,and so on. Conclusion: Use high quality clinical trials to further prove the clinical effects and safety of tripterygium for AS is necessary promptly.关键词：ankylosing spondylitis;tripterygium;randomized controlled trials;systematic reviews
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发布时间：2024-01-04 - 摘要：Objective: To investigate the four property and five tastes in the properties. Method: Using Chinese medicine herb properties and clinical drug application theory, by means of the literature data and mining method, summarize the four property and five tastes of the plant parasitic Yi medicine in the literature of Yi medicine books which still exist. Result: Constituent ratio of the four property, warm natured>cold natured=cool natured>‘ping’ natured>hot natured. Constituent ratio of the five tastes, bitter>astringent>arid=acid>sweet=thin>saline. From the perspective of combination, 7 medicines have the taste of bitter combining with astringent, there is 1 medicine has the taste of bitter combining with acrid, acid combing with bitter, acid combing with astringent respectively;there is 1 medicine has the tastes of acid combining with bitter and astringent, bitter combing with acrid and astringent respectively. Conclusion: Four property:the number of warm natured drugs is most in four property, the following is cold or cool, ‘ping’ is least and no hot natured drugs.Five tastes:five taste has single, two taste and three flavor combinations, including single flavor with bitter most, no saline medicine. Most in two tastes is bitter combing with astringent. Three flavor combinations have acid bitter astringent and bitter acrid astringent.关键词：plant parasitic Yi medicine’s;property;four property;five tastes
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发布时间：2024-01-04
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