最新刊期
卷 19 ， 期 23 ， 2013
- 摘要：Objective: To optimize diphasic dynamic extraction technology of quercetin from Flos Sophorae Immaturus. Method: With the content of quercetin as index,based on single factor tests,L9(34) orthogonal test was adopted to optimize diphasic dynamic extraction technology of quercetin by taking extraction time,extraction temperature,extraction times and sulfuric acid concentration as factors.The content of quercetin was determined by HPLC,chromatographic conditions were as follows:Agilent ZORBAX C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.02% H3PO4(45:55),detection wavelength 254 nm,injection volume 10 μL. Result: Optimal extraction technology was as following:extracted 2 times with 2% sulfuric acid at 85℃,2.5 hours per time;Yield of quercetin was up to 5.22 mg· g-1,which was more than ethanol extraction method of 3.26 mg· g-1. Conclusion: This optimized extraction technology was feasible and stable with high yield of quercetin.关键词：Flos Sophorae Immaturus;quercetin;extraction technology;diphasic dynamic extraction;orthogonal test;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To optimize separation and purification technology of total flavonoids from ethanol extract of Ziziphi Spinosae Semen(ZSS) by macroporous adsorption resin. Method: Adsorption and desorption properties of six different types of macroporous resins for total flavonoids from ZSS were compared by static and dynamic adsorption and desorption tests to select optimum type of macroporous resin;With the content of total flavonoids as index,single factor tests were adopted to investigate adsorption and elution conditions of macroporous resin,such as the concentration of sample diluted,adsorption rate,eluent concentration and so on. Result: SP-207 macroporous resin was adopted,optimum separation and purification conditions for total flavonoids from ZSS were as follows:the concentration of sample solution 0.5 g· mL-1,adsorption rate 1 BV· h-1,sample volume 5 BV,eluted by 4.5 BV of 70% ethanol at a flow rate of 4 BV· h-1;The content of total flavonoids from extract of ZSS was 65.47%. Conclusion: This optimized process was stable,feasible and suitable for separation and purification of total flavonoids from ZZS.关键词：Ziziphi Spinosae Semen;total flavonoids;macroporous adsorption resin;separation and purification technology;static adsorption and desorption test;dynamic adsorption and desorption test;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of rosmarinic acid in Melissa officinalis. Method: With the content of rosmarinic acid as index,based on single factor tests,effects of extraction time,extraction times and the amount of ethanol on extraction technology were investigated by orthogonal test.HPLC was employed to determine the content of rosmarinic acid and the chromatographic conditions were as follows:Hanbon Megres C18 column(4.6 mm×250 mm,5 μm),mobile phase of formic acid-acetonitrile-water(0.5:20:80,A) and formic acid-methanol-acetonitrile(0.5:40:60,B) with gradient elution program(0-25 min,0%-45% B;25-30 min,45%-100% B),detection wavelength 330 nm,injection volume 20 μL. Result: Optimal extraction technology was as following:extracted 3 times with 10 times the amount of 70% ethanol,1 h each time;Yield of rosmarinic acid was up to 25.27 mg· g-1. Conclusion: Optimized technology was stable and feasible to provide a reference for resource development of M. officinalis.关键词：Melissa officinalis;rosmarinic acid;extraction technology;single factor test;orthogonal test;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize water extraction technology and molding process of Tangzhiping granules. Method: With the content of total polysaccharides form Hedysari Radix as index,L9(34) orthogonal test was used to optimize water extraction process of Tangzhiping granules by taking water amount,extraction time and times as factors;With moisture absorption rate,formability,angle of repose and granulation as indexes,single factor tests were adopted to investigate effects of kind and amount of diluents,flavoring agents and wetting agents on molding technology.Angle of repose was determined by fixed funnel method. Result: Optimum water extraction process was as following:extracted 2 times with 10-folds the amount of water,2 h per time.Spray drying process was as following:the relative density of extract 1.05-1.10(60℃),material temperature 50-60℃,inlet temperature 170℃,outlet temperature 85℃;Ultrafine grinding of Hirudo and Bombyx Batryticatus,volatile oil from Moutan Cortex was prepared β-CD clathrate,with 91% ethanol as wetting agent,powder-diluent(1:0.3),flavoring agent 2%. Conclusion: Optimized process was reasonable,feasible and applicable to preparation of Tangzhiping granules.关键词：Tangzhiping granules;extraction technology;spray drying;ultrafine grinding;orthogonal test;accessories;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of volatile oil from Cortex Cercis Chinensis by central composite design and response surface methodology. Method: With yield of volatile oil as dependent variable,based on single factor tests,central composite design-response surface methodology was adopted to optimize extraction technology by taking soaking time,extraction time and the amount of water as independent variables,through multiple linear regression and binomial fitting of each level of independent variables to prediction analysis of extraction technology. Result: Optimum process conditions were as follows:soaked 1 h with 14 times the amount of water,extraction time 5 h;Yield of volatile oil was 0.8%,average mass of volatile oil was 0.391 2 g with a small deviation between the predicted value(0.391 5 g). Conclusion: This optimized extraction process was simple and reasonable with a good predictability.关键词：Cortex Cercis Chinensis;central composite design-response surface methodology;steam distillation method;single factor test;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of total triterpenoid acids from Elaeagnus bockii by macroporous resin. Method: The content of total triterpenoid acids was determined by UV.Type of macroporous resin was screened by dynamic adsorption-elution test;With the content of total triterpenoid acids as index,influence factors were selected by single factor tests,main factors were investigated by Plackett-Burman.Taking composite score of the content of total triterpenoid acids and extract yield as index,L9(34) orthogonal test was applied to optimize purification process of total triterpenoid acids with ethanol concentration,alcohol amount,ratio of ethanol and 1% sodium hydroxide as factors. Result: Optimum purification process conditions were as follows:eluent amount 4 BV,ratio of 80% ethanol and 1% NaOH (1:5),flow rate 3 mL· min-1;The mass fraction of total triterpenoid acids was 75%,extract yield was more than 22%. Conclusion: This optimized process was simple and stable to provide experimental basis development and utilization of total triterpenoid acids from E. bockii with high precision and predictability.关键词：Elaeagnus bockii;total triterpenoid acids;Plackett-Burman;orthogonal test;purification process;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To optimize percolation process of Valeriana officinalis dregs in compound V. officinalis toothache tinctures with pharmacodynamic indicators. Method: Changes of capillary permeability of mouse ear inflammation model was adopted,pharmacodynamic indicators of percolation process end-product of V. officinalis dregs were compared,effects of ethanol concentration,soaking time,ethanol amount and flow rate on percolation process were investigated by orthogonal test. Result: Pharmacodynamic effects of each factors on percolation process of V. officinalis dregs was in order of ethanol concentration>soaking time>ethanol amount>flow rate,optimum percolation process was as following:soaked 1 h with 15 times the amount of 75% ethanol at 3.0 BV· h-1. Conclusion: Optimized percolation process parameters were stable and reliable through pharmacodynamic tests,they had practical guiding significance.关键词：Valeriana officinalis;percolation process;pharmacodynamic indexes;orthogonal test;absorbance
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发布时间：2024-01-04 - 摘要：Objective: To optimize microwave extraction process of effective parts in Caulis Polygoni Multiflori for improving sleep. Method: Single-factor tests were adopted to investigate effects of extraction time,ethanol concentration,microwave power,solvent amount and extraction temperature on contents of total flavonoids and total anthraquinones in Caulis Polygoni Multiflori.Based on single factor tests,taking extraction time,ethanol concentration and microwave power as factors,each factors was set five levels,with the "Overall Desirability" of contents of total flavonoids and total anthraquinones as evaluation indicator,then extraction process of effective parts in Caulis Polygoni Multiflori for improving sleep was optimized by central composite design-response surface methodology. Result: Optimum microwave extraction conditions were as follows:microwave power 585 W,extracted 11 min with 10 times the amount of 82% ethanol at 60℃,extracted 1 time. Conclusion: Optimized extraction technology was simple and feasible with a good predictability,it could provide experimental evidence for formulations development and industrial production of Caulis Polygoni Multiflori.关键词：Caulis Polygoni Multiflori;effective part for improving sleep;total flavonoids;total anthraquinones;microwave-assisted extraction process;central composite design-response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation of Jiangjiao gels and investigate its in vitro percutaneons penetration. Method: Taking cumulative permeation amount per unit area in 24 h of capsaicin as index,effect of dosages of glycerol,propylene glycol and azone on formulation process of capsaicin was investigated by orthogonal test;With isolated rat skin as transdermal barrier,Franz diffusion cell was used as in vitro percutaneous release model,then in vitro transdermal permeability of capsaicin was investigated.The content of capsaicin was determined by HPLC,chromatographic conditions were as follows:Diamonsil C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.1% phosphoric acid solution(65:35),flow rate 1.0 mL· min-1,detection wavelength 280 nm,column temperature 40℃,injection volume 20 μL. Result: Optimal formulation of Jiangjiao gels was 2% carbomer,10% glycerol,20% propylene glycol and 1% azone;In vitro transdermal permeation of capsaicin in Jiangjiao gels fitted zero order kinetics equation,percutaneous permeation rate was 0.353 μg· h-1· cm-2. Conclusion: Optimized formulation was reasonable and suitable for topical skin.关键词：Jiangjiao gels;capsaicin;percutaneous penetration;orthogonal test;formulation process;cumulative permeation amount per unit area
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发布时间：2024-01-04 - 摘要：Objective: To optimize machine decoction process of Huanglian Jiedu decoction. Method: With composite score of baicalin,berberine and extractum contents as index,effects of soak time,decoction time and decoction temperature on machine decoction process were optimized by single factor tests and orthogonal test,and compared with traditional decoction process.Contents of index components were determined by HPLC,chromatographic conditions were as follows:YMC-Pack ODS-A column(4.6 mm×250 mm,5 μm),mobile phase of methanol-0.2% phosphoric acid(47:53),detection wavelength 250 nm. Result: Optimal machine decoction process was as following:Soaked 10 minutes,decoction time 30 minutes,decoction temperature 105℃;it was better than traditional decoction process. Conclusion: Optimized machine decoction process was reasonable and feasible with controllable evaluation index,it could provide experimental basis for developing of machine decoction process of Chinese medicine decoctions.关键词：Huanglian Jiedu Decoction;Orthogonal Design;HPLC;machine decoction process;difference;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To optimize chitosan flocculant clarification process of Dantao granules and compare with ethanol precipitation method and centrifugation method. Method: With yield of water extract,retention rates of amygdalin and paeoniflorin as comprehensive evaluation index,orthogonal test was adopted to optimize chitosan flocculant clarification process of Dantao granules with chitosan dosage,flocculation liquid temperature and pH of liquid as factors,three clarification processes including ethanal precipitation method,adsorption clarification technique,high-speed centrifugation method were compared with nature sedimentation. Result: Optimized chitosan flocculant clarification process was as following:added 10% liquid volume of 1% chitosan,pH of liquid 6.0,flocculation liquid temperature 50℃;Water extract amount reduced to around 19%,retention rate of amygdalin and paeoniflorin were more than 89%,it was superior to ethanol precipitation method and centrifugation method. Conclusion: Chitosan flocculant clarification could be used as clarification process of Dantao granules,and this optimized technique was reasonable,stable and feasible for industrial production.关键词：Dantao granules;clarification process;chitosan;orthogonal test;multi-weighted index comprehensive evaluation method
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology conditions of total flavonoids from leaves of Broussonetia papyrifera by macroporous adsorption resin. Method: With total flavonoids from leaves of B. papyrifera as index,type of macroporous resin was screened by by static adsorption-desorption and dynamic adsorption-desorption tests,effects of eluent consumption,elution flow rate,the concentration of sample solution and other factors on purification technology were investigated by single factor tests. Result: HPD450 was selected for its excellent adsorption and desorption properties,optimum process was as following:the concentration of sampling solution 4.4 g· L-1,sampling velocity 1 BV· h-1,sample liquid volume 6 BV,eluted by 5 BV deionized water and 8 BV 50% ethanol at speed of 1 BV· h-1,diameter to height ratio of resin 1:8,collected eluate.Under these conditions,purity of total flavonoids was up to 75.75% from 30.65%,retention rate of total flavonoids reached 79.89%,impurity removal rate reached 64.88%. Conclusion: HPD450 macroporous resin was suitable for separation and purification of total flavonoids from leaves of b. papyrifera,optimized purification technology was stable and feasible,it could be extended for industrial production applications.关键词：macroporous resin;leaves of Broussonetia papyrifera;total flavonoids;adsorption;separation and purification technology;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro release properties and gastric residence time in rabbits of four ingredients from Wuji gastric floating sustained-release tablets. Method: In vitro release of Wuji gastric floating sustained-release tablets was determined by slurry method,with 0.1 mol· mL-1 HCl solution containing 0.8% tween-80 as release mediator,cumulative release of paeoniflorin,berberine hydrochloride,evodiamine and rutaecarpin from Wuji gastric floating sustained-release tablets within 12 h were measured by HPLC,chromatographic conditions were as follows:Thermo-ODS2-Hypersil column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile(A)-0.05 mol· L-1 potassium dihydrogen phosphate(B) with gradient elution program of (0-20 min,11% A;22-44 min,28% A;46-70 min,40% A),detection wavelength 225 nm;Dissolution kinetics models were simulated by lognormal distribution model,Weibull function and Higuchi equation,and γ scintigraphy was adopted to detect gastric residence time in rabbits of Wuji gastric floating sustained-release tablets. Result: Cumulative release of four ingredients all fitted Higuchi equation,gastric residence time of this preparation was 6 h in rabbits. Conclusion: Under satiety state,gastric residence time in rabbits of Wuji gastric floating sustained-release tablets was significantly longer than fasting state,it revealed that food may have a significant impact on gastric residence time of this tablets.关键词：Wuji gastric floating sustained-release tablets;release;release mechanism;γ scintigraphy;in vivo residence time
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发布时间：2024-01-04 - 摘要：Objective: To analysis feasibility of matrix solid-phase dispersion technology for extraction of arctiin and arctigenin from Arctii Fructus. Method: With total extraction amount of arctiin and arctigenin as index,which were determined by HPLC,effects of dispersants,eluents,compaction degree and elution flow rate on extraction rate of active ingredients from Arctii Fructus were investigated by single factor tests,and compared with methanol ultrasonic extraction technology. Result: Optimum matrix solid-phase dispersion technology was as following:with four times the amount of silica gel as dispersants,one hundred times the amount of methanol as eluents,compaction degree moderate extrusion,elution flow rate 40 drops/min. Total extraction ratio of arctiin and arctigenin was up to 7.94%,while it was 7.89% by methanol ultrasonic extraction technology. Conclusion: Matrix solid-phase dispersion method was simple and rapid,it could be used as a new method for extracting main ingredients from Arctii Fructus and provide a reference for extraction of active ingredients from Chinese herbal medicines.关键词：matrix solid-phase dispersion method;Arctii Fructus;arctiin;arctigenin;HPLC;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro transdermal absorption ability of active ingredients from ginseng extract and ginseng creams. Method: Taking intelligent transdermal absorption instrument,with total saponins and total polysaccharides from ginseng Radix et Rhizoma as indexes,which were determined by UV;Before and after ginseng extract were prepared into ginseng creams,percutaneous absorption capacity changes of index components were investigated by in vitro release test. Result: Transdermal absorption process of total saponins from ginseng creams was in line with the first-order equation,but total polysaccharides from ginseng creams and total saponins and total polysaccharides from ginseng extract were in line with Higuchi release equation;After been prepared into ginseng creams,cumulative release rates of these two ingredients were 82.30% and 67.53%,respectively. Conclusion: ginseng creams had good transdermal ability,it could increase transdermal absorption of effective components and provide a scientific basis for research of other transdermal formulations.关键词：Ginseng Radix et Rhizoma;creams;extract;transdermal penetration effect;in vitro release characteristics
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification process of total polyphenols from acid Granti Pericarpium in Kashi. Method: With the content of total polyphenols as index,type of macroporous resin was screened by static adsorption-elution test,single factor tests were adopted to optimize purification process of total polyphenols by taking sample solution concentration,eluent consumption,adsorption speed and others as factors. Result: HPD500 macroporous resin was selected,optimum purification process was as follows:the crude drug concentration of sample solution 20.8 g· L-1,pH of sample solution 2,sample volume 8 BV,adsorption rate 2 BV· h-1,adsorption time 12 h,eluted impurities with 7 BV of distilled water and 8 BV of 50% ethanol,collected ethanol eluate;Purity of total polyphenols was up to 66.93% from 24% before purification,yield of extract was 34.80%. Conclusion: HPD500 macroporous resin was suitable for purification of total polyphenols from acid Granti Pericarpium,optimized process conditions were simple and feasible with high efficiency and low toxicity.关键词：acid Granti Pericarpium in Kashi;total polyphenols;effective fraction;purification process;static adsorption-elution test;Macroporous resin
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发布时间：2024-01-04 - 摘要：Objective: To compare effects of traditional decoction method and modern decoction method on active ingredients yield and extract yield of Gegen Qinlian decoction. Method: Taking yield of dry extract and contents of twelve active components as indexes,different values of medication in classical formulas included one ounce equals to13.8 g and one ounce equals to 3 gram were adopted,effects of two different decoction methods on quality of Gegen Qinlian decoction were investigated by single factor tests. Result: When one ounce equals to13.8 g, compared with modern decoction method,contents of active components by traditional decoction method reached 47%-74%,dry extract rate was 67%;When one ounce equals to 3 g,compared with modern decoction method,contents of active components by traditional decoction method reached 102%-148%,dry extract rate was 118%. Conclusion: Different decoction methods with different values of classic formulas had effects on contents of active components,this study could provide a reference for making the past serve the present of classical prescriptions.关键词：Gegen Qinlian decoction;decoction process;medicinal components;determination;dry extract rate
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发布时间：2024-01-04 - 摘要：Objective: To use fast liquid chromatography-triple quadrupole-time of flight mass spectrometry (UFLC-Q-TOF/MS) for analyzing the chemical constituents of flavonoids in traditional Chinese herbal medicine Hedyotis diffusa. Method: The separation was performed on a ACQUITY UPLC BEH C18 Column (2.1 mm×100 mm,1.7 μm) with a mobile phase using 0.1% formic acid ACN (A) and water containing 0.1% formic acid (B)for gradient elution. Time-of-flight mass spect-rometer (TOF/MS) and electrospray ion source (ESI) was applied for qualitative analysis under the negative ion mode.The flow rate was 0.3 mL· min-1,the temperature of column was 40℃. and mass scan range was m/z 100-1 500. Result: Fifteen compounds were identified from flavonoids of H. diffusa. Conclusion: To use UFLC-Q-TOF/MS method can quickly identify the chemical components of H. diffusa which paves a way for further studying the pharmacodynamic material basis of H. diffusa.关键词：Hedyotis diffusa;flavonoids;UFLC-Q-TOF-MS;compounds
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发布时间：2024-01-04 - 摘要：Objective: To study the correlation between active components and Endophytic fungal community in Salvia mihiorrhiza from different habitats. Method: Tissue separation is applied to separate the E. fungi in roots of S. mihiorrhiza. The fungi are classified and identified by insert training methods and point-sik methods. The content of tanshinoneⅡA,cryptotanshinone,salvianolic acid B and danshensu are determined by HPLC. Result: E. fungal are abundance in species and quantity,which are generally found in the roots of S. mihiorrhiza from different habitats,are identified into 3 orders,4 families and 36 genera.The correlation between active components and E. fungi in S. mihiorrhiza was revealed. Conclusion: Endophytic fungi in S. mihiorrhiza from different habitats in terms of quantity,distribution,populati on and its components are different,and the correlation between E. fungal and their quality formation is revealed.关键词：Salvia mihiorrhiza;endophytic fungi;tanshinoneⅡA;salvianolic acid B;Cryptotanshinone;danshensu;correlation
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Voacanga africana. Method: The compounds were isolated by normal phase silica gel,ODS,and sephadex LH-20 column chromatography. Their structures were elucidated by analysis of spectroscopic data. Result: Fourteen compounds were isolated and their structures were elucidatedas3-OAc,Δ7,bauerenylacetate(1),β-sitosterol(2),voacangine(3),8-hydroxy-6-methoxy-3-pentylisocoumarin(4),voacristine(5),voacamine(6),22E,24R-5α,8α-epidioxyergosta-6,22-dien-3β-acetate(7),voacristinehydroxyindolenine(8),voacryptine(9),oleanolicacid(10)β-amyrin(11),(22E)-5a,8a-epidioxyergosta-6,22-dien-3β-ol(12),6β-hydroxystigmast-4-en-3-one(13),palmiti acid( 14 ). Conclusion: Except compound 3,5,6,9,the other ten compounds are isolated from this plant for the first time;Compound 1,4,7,12,13 were isolated from this genus Voacanga for the first time;Compound 4 is the frist coumarins isolated from the species V. africana Staph.关键词：Voacanga africana;chemical constituents;alkaloid;Apocynaceae;Voacanga
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发布时间：2024-01-04 - 摘要：Objective: To identify chemical components in volatile oil from Machilus salicina Hance and determine their relative contents. Method: Volatile oil from fresh leaves of M. salicina Hance was extracted by hydrodistillation.GC-MS was adopted to analyze chemical constituents in volatile oil and determined their relative contents,mass spectrometric conditions were as follows:HP-FFAP quartz capillary column(0.25 mm×30 m,0.25 μm),the starting temperature 40℃,heated to 200℃ with 5℃· min-1,maintained 5 min,then with 8℃· min-1 heated to 280℃ until completion of analysis,helium as the carrier gas(99.999% ),column flow rate 1.0 mL· min-1,inlet temperature 250℃,split ratio 50:1,EI ionization source 70 eV,ion source temperature 230℃,quadrupole temperature 180℃,solvent delayed 2.5 min,scan range of m/z 50-550. Result: Yield of volatile oil was 1.05%,68 kinds of compounds were obtained and 55 kinds of compounds(accounted for 77.10% of total mass of volatile oil) were identified,26 of them was olefinic compounds,12 of them was alcohol compounds,the concentration of (Z)-nerolidol,spoon eudesmol and ledol were 12.62%,7.48% and 5.38%,respectively. Conclusion: GC-MS could be used to identify and determine content of volatile components,this study could provide experimental basis for development and utilization of M. salicina Hance.关键词：Machilus salicina;volatile oil;chemical constituents;GC-MS;spoon eudesmol;ledol
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprint of Rehmannia glutinosa to provide a reliable method for scientific evaluation and the effective control of its quality. Method: The fingerprints were obtained on a Dikma Diamonsil C18column (4.6 mm×250 mm,5 μm) with the gradient elution solvent system composed of acetonitrile and 0.1% phosphoric acid. The flow rate was 1.0 mL· min-1. The column temperature was maintained at 35℃,and the detection wavelength was set at 240 nm. Result: Thirteen common peaks were selected as the fingerprint peaks and the fingerprints were also evaluated by the Similarity Evaluation System for Chromatographic Fingerprint with the correlation coefficient above 0.99. Conclusion: This method with good precision and reproducibility is reliable for the quality control of R. glutinosa.关键词：Rehmannia glutinosa;HPLC;fingerprint;clueter analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint analysis methods for Madder and to provide the reference for quality evaluation of Madder. Method: Phenomenex ODS C18(4.60 mm×250 mm,4 μm)column was used and the methanol-0.2% H3PO4 water was chosen as the mobile phase in a gradient mode. The column temperature was at 25℃ and the detection wavelength was at 275 nm.The detection time was 80 min and the flow rate was 0.8 mL· min-1. Result: Taking mollugin as the reference peak,eleven common peaks were selected as the fingerprint peaks of Madder. Conclusion: This method is simple and accurate with a good reproducibility. It provides a reference standard for the quality control of Madder.关键词：Madder;mollugin;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To establish a simultaneous determination of Cl-,Br-,NO3-,SO42-,PO43- content of gardenia by ion chromatography. Method: The ICS-900 ion chromatography and IonPac AS11-HC inorganic anion exchange chromatography column,KOH gradient elution and conductivity detection were adapted to assay the five kinds of inorganic anion. Result: Cl-,Br-,NO3-,SO42-,PO43- were good in linearity. Samples recovery were between 97%-102%. The content of Cl-,Br-,NO3-,SO42-,PO43- were 0.084%,0.063%,0.070%,0.30% and 0.12%. Conclusion: The method is sensitive,accurate and operational,provided a simple and quickly method to assay the content of Cl-,Br-,NO3-,SO42-,PO43- in gardenia.
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of gallic acid and ellagic acid and establish fingerprints to assess the quality of Phyllanthi Fructus derived from thirteen batches of Phyllanthus emblica,which is in order to establish the quality evaluation of Phyllanthi Fructus. Method: HPLC fingerprinting and content determination methods were applied to evaluate thirteen batches of Phyllanthi Fructus. The samples were separated by an Alltima C18 column (4.6 mm×250 mm,5 μm) using acetonitrile-0.2% phosphoric acid with water gradient system as mobile phase.The flow rate was 1.0 mL· min-1,the detection wavelength for fingerprinting was at 273 nm,and the wavelength for content determination of gallic acid and ellagic acid were at 273 nm and 254 nm respectively. Result: HPLC fingerprint of Phyllanthi Fructus was established with good separation and repeatability,which could be used for quality assessment of Phyllanthi Fructus. The results showed that the HPLC fingerprints were similar among thirteen batches samples of Phyllanthi Fructus,but significantly different between Hong Kong samples and mainland samples. The content of gallic acid and ellagic acid from Hong Kong and mainland was obviously different from each other. The HPLC fingerprints of herbal Phyllanthi Fructus and ripe fruity of P. emblica were similar,while the content of gallic acid and ellagic acid was obviously different. Conclusion: The method is sensitive,repeatable and accurate,it can be used as quality control for Phyllanthi Fructus and has been adopted in "Hong Kong Chinese Materia Medica Standards".The content of gallic acid and ellagic acid varied significantly in Phyllanthi Fructus from different habitats which suggested that more attention should be paid to clinical use of different Phyllanthi Fructus (including herbal Phyllanthi Fructus and ripe fruity of P.emblica) in practice.
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发布时间：2024-01-04 - 摘要：Objective: To analyze the content of heavy metals and harmful elements in Holotrichia diomphalia and provide the scientific foundation for the quality control and standard establishment of H. diomphalia. Method: The content of Pb,Cd,Cu,As,Hg constituents was determined by ICP/MS after microwave-assisted digestion and atom absorption spectrophotometry. The Chinese pharmacopoeia(Volume 1) of 2010 edition was used as the standard to evaluate the pollution condition of Pb,Cd,Cu,As,Hg in H. diomphalia. Result: The content of Pb,Cd,Cu,As,Hg constituents determined by ICP/MS had not obvious difference with the ones determined by atomabsorption spectrophotometry. The content of Pb,Cd,Cu,As and Hg determined by ICP/MS was 3.912,0.163,17.386,1.983 and 0.260 mg· kg-1 respectively. The content of Pb,Cd,Cu,As and Hg determined by atom absorption spectrophotometry was 3.777,0.155,18.027,2.044 and 0.252 mg· kg-1 respectively. Conclusion: The concentrations of Cd in the samples from five different areas of H. diomphalia were lower than the limits of Chinese pharmacopoeia,but the concentrations of Pb,Cu,As,Hg in the some samples from different areas were more than the limit standard. The result is helpful to develop the quality standards of H. diomphalia four harmful elements.关键词：Holotrichia diomphalia;heavy metal;harmful element;ICP/MS;atom absorption spectrophotometry
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for the determination of ursolic acid content in Eriobotrya japonica by near-infrared spectroscopy. Method: The ursolic acid contents of 131 samples were determined by the method of HPLC. NIR spectrograms were measured,the second derivative was used as spectral preprocessing options,and calibration model of the ethanol extracts content was established by the partial least squares regression analysis,The models were verified by internal cross validation and external predictive validation with 25 E. japonica samples. Result: The correlation coefficients,the root-mean-square error of calibration,the root-mean-square error of prediction and the toot-mean-square error of cross-validation of the calibration model for ethanol extracts content were 0.982 46,0.175,0.249 and 0.525 02. Conclusion: The method is steady,accurate,and can be used to predict ethanol extracts content of Eriobotrya japonica rapidly.关键词：Eriobotrya japonica;HPLC;ursolic acid content;NIRS
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for the determination of procymidone residues in ginseng extract. Method: The BDS HYPERSIL C18 (4.6 mm×250 mm,5 μm) column was used as analytical column with the mobile phase of acetonitrile-water (40:60) at the flow rate of 1.0 mL· min-1,and the detective wavelength was set at 220 nm. Result: Good linear range was obtained for the procymidione with an injection amount ranging from 0.001 to 0.6 μg (R2=0.999 4). The average recovery was 100.0% (n=9),and RSD was 3.0%. Conclusion: The HPLC method is simple,accurate and effective,which can be used for the detection of procymidone pesticide residues in ginseng extract.关键词：ginseng extract;procymidone;HPLC;pesticide residues
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of phillyrin,forsythoside A and ethanol-extract in green Forsythiae Fructus by near-infrared spectroscopy. Method: Combine the near-infrared spectroscopy with partial least squares (PLS) to establish the quantitative model of phillyrin,forsythoside A and ethanol-extract in grown Forsythiae Fructus. Result: The correlation coefficients (R2) of phillyrin,forsythoside A and ethanol-extract were respectively 0.957 33,0.986 93 and 0.992 62.The validation coefficients (r) were respectively 0.941 9,0.976 1 and 0.989 7. Conclusion: The method is convenient and accurate and it's feasible to determine the content of phillyrin,forsythoside A and ethanol-extract in green Forsythiae Fructus by near-infrared spectroscopy.关键词：near-infrared spectroscopy;PLS;green Forsythiae Fructus;Determination
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发布时间：2024-01-04 - 摘要：Objective: To determine moisture in Fructus Corni rapidly by Near infrared Spectroscopy. Method: Drying method was used as a reference method to determine the content of moisture in Fructus Corni. In this study,NIR spectra of the powdered Fructus Corni were collected,and the PLS was developed to correlate the spectra and the corresponding values determined by the reference method. Result: The correlation coefficient(R2) of the calibration model of NIR Spectroscopy for moisture was 0.990 52,the root-mean-square error of prediction was 0.297,the prediction mean square error was 0.819. Conclusion: The parameters of the model were also discussed. The overall results show that NIR spectroscopy combined with PLS can be efficiently utilized for rapid and accurate analysis of water in Fructus Corni. Simultaneously,the research can serve as the technical support and the foundation of quantitative analysis of other NIR applications.关键词：near infrared Spectrum;partial least square;Fructus Corni;moisture;rapid determination
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发布时间：2024-01-04 - 摘要：Objective: To investigate determination of total iridoid glycoside,total flavonoids and total phenethanol glycosides from different extractive of extract of Lamiophlomis rotata. Method: Water extract of L. rotata were separated and enriched obtain water eluate and 70% ethanol solution eluateⅠ(70% ethanol solution eluted polyamide column),70% ethanol solution eluate Ⅱ (70% ethanol solution eluting macroporous resin column) by polyamide and macroporous resin. Total iridoid glycosides ingredients were assayed by the first derivative spectrophotometry;the total flavonoids and total benzene glycosides were assayed by dual wavelength UV spectrophotometry,aluminum nitrate-sodium nitrite UV spectrophotometry. Result: Recoveries of the total iridoid glycosides was 104.40%,RSD was 0.64% at 252 nm wavelength by the first derivative UV spectrophotometry. Recoveries of the total flavonoids were 96.23% and 104.48%,RSDs were 7.27% and 3.61% at the 268 nm and 363 nm wavelengths by UV spectrophotometry. Recoveries of the total benzene glycosides was 100.26%,RSD was 2.92% at 332 nm wavelength. Conclusion: The first derivative ultraviolet spectrophotometry can determine the total iridoid glycosides. The ultraviolet spectrophotometry can determine the total flavonoids and total benzene glycosides.关键词：Lamiophlomis rotata;total iridoid glycosides;total flavonoids;total phenylethanol glycosides
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发布时间：2024-01-04 - 摘要：Objective: To compare and analyze the volatile oil components of Guangxi camphora in different parts between fresh and dry herbs. Method: The volatile oil components were extracted by steam distillation from the leaf,branch,root of fresh and dry Guangxi camphora,and the chemical components of abstracted volatile oil was tested by GC-MS,the relative content of components was computed by area normalization method. Result: 17,27,14 components were identified from fresh leaf,branch,root of Guangxi camphora,which accounting for 85.13%,72.94%,99.74% of the total volatile oil. 15,32,18 components were identified from dry leaf,branch,root of Guangxi camphora,which accounting for 75.44%,70.82%,97.73% of the total volatile oil. Conclusion: Volatile oil components were different,which provide scientific basis for formulating quality standard of Guangxi camphora and further development.
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发布时间：2024-01-04 - 摘要：Objective: To research the dissolution rate of the total ginsenoside of Panax ginseng and A. sinensis in different proportion after alcohol decocting,and discuss the compatibility mechanism of action and reflect the integrity theory of TCM. Method: The content of total ginsenoside in the alcohol extracts of P. ginseng and A. sinensis in different proportion was measured by the method of UV. Result: The content of total ginsenoside has degraded after P. ginseng and A. sinensis compatibility of different proportion in alcohol decocting. The different degrees varied from different proportions,1:1 proportion resulting in the highest reducing rate. Conclusion: The change of total ginsenoside after compatibility of different proportion of P. ginseng and A. sinensis can provide the scientific basis of the two drugs for compatibility use.关键词：Panax ginseng;Angelica sinensis;total ginsenoside;UV
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for the quality control of genistein vaginal tablets. Method: The Chinese Pharmacopoeia pH determination method was used for determination pH of genistein vaginal tablets. Genistein in genistein vaginal tablets was identified by TLC. The content of genistein in genistein vaginal tablets was determined by HPLC. Result: TLC spots were clear with high resolution and strong specialization. Genistein presented a good linear correlation in the range of 0.201 2-1.006 0 μg (r=0.999 9). The average recovery was 97.72% with RSD of 1.65% (n=6). The pH and the content of genistein in genistein vaginal tablets in different batches had no significant differences. Conclusion: The developed method was simple,accurate and repeatable. It can be used for the quality control of genistein vaginal tablets.关键词：genistein;vaginal tablets;quality control;pH;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of phellodendrine,magnoflorine,palmatine,berberine and obakunone in Phellodendron chinense var glabriusculum and P. chinense. Method: The determination was carried out with RP-HPLC on Xtimate C18 column (4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile (A)-0.1% phosphate (B) by gradient elution and the flow rate was 1.0 mL· min-1. The detection wavelength was set at 220 nm at 30℃. Result: The five components separated well under the above conditions. Phellodendrine,magnoflorine,palmatine,berberine and obakunone had good linear relationship within the ranges of 0.003 5-0.07 g· L-1 (r=0.999 9),0.004-0.08 g· L-1 (r=0.999 9),0.001-0.02 g· L-1 (r=0.999 9),0.01-0.40 g· L-1 (r=0.999 5),0.001-0.02 g· L-1(r=0.999 5). The average recoveries were greater than 97%,(RSD<3%,n=9). Conclusion: The method is simple,accurate and repeatable,and can be used as the quality control method of P. chinense and P. chinense var glabriusculum. There are no significant differences between P. chinense and P. chinense var glabriusculum in terms of five kinds of chemical composition content. P. chinense var glabriusculum has become mainstream products. So it is recommended that P. chinense var glabriusculum should be back into the source of Phellodendri Chinensis Cortex.关键词：Phyllodendron chinense Schneid.var.glabriusculum Schneid.;P. chinense Schneid.;HPLC;determination
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发布时间：2024-01-04 - 摘要：Objective: To study the constituents of the roots of Disporum cantoniense in extracting with ethyl acetate. Method: The compounds were separated and purified by silica gel and recrystallisation. Their structures were identified by their physico-chemical properties and spectroscopic data. Result: Six compounds were isolated and identified as physcion(1),emodin(2),stigmasterol(3),quercetin(4),β-sitosterol(5),daucosterol(6). Conclusion: Compounds of 1-4 were obtained from this plant for the first time.关键词：Disporum cantoniense;chemical constituents;structure identification;anthroquinones
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发布时间：2024-01-04 - 摘要：Objective: To study the volatile components from Qinghai wild Mentha hapioealyx. Method: The essential oil of wild Mentha haplocalyx in Qinghai is extracted by steam distillation,volatile components were analysed by GC-MS,and 84 compounds were separated and 42 main volatile compounds were identified. Result: Compounds with high contents were as follows:cyclohexanone,5-methyl-2-(1-methylethyl)-,trans-(26.75% ),2-cyclohexen-1-one,3-methyl-6-(1-methylethyl)-(16.61% ),D-limonene(6.00% ),caryophyllene(4.26% ),.alpha.-cadinol(4.10% ),1,6-cyclodecadiene,1-methyl-5-methylene-8-(1-methylethyl)-, [s-(E,E)]-(3.63% ). Conclusion: Since there are abundant chemical component and differente from interior variety,it is worthy of exploitation and protection.关键词：Mentha haplocalyx;GC-MS;essential oil
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standards for Chinese medicine processed Aconitum carmichneli (Tuber of Aconitum armichaeli). Method: Benzoylmesaconine was identified by TLC method. Monoester alkaloids and diester type alkaloids were determined by HPLC method. HPLC condition was as follows:The Eclipse XDB-C18 (4.6 mm×250 mm,5 μm) column was used;the column temperature was maintained at 35℃. The mobile phase system was 0.1 mol· L-1 ammonium acetate (per 1 000 mL contains 0.5 mL glacial acetic acid,phase A) and tetrahydrofuran-acetonitrile (15:25,phase B) with gradient elution. The flow rate was 1 mL· min-1. The detection wavelength was set at 235 nm. Result: The water content was ranged from 10.1% to 12.3%. The qualitative identification was specific. Benzoylmesaconine,benzoylaconine,benzoylhypaconine,mesaconitine,hypaconitine and aconitine showed good linear relationship in the range of 0.049 4-1.976,0.023 32-0.932 8,0.049 95-1.998,0.024 46-0.978 56,0.024 91-0.996 48,0.022 02-0.880 8 μg respectively (r=0.999 9 for all six substances). The average recovery rates of benzoylmesaconine,benzoylaconine,benzoylhypaconine,mesaconitine and hypaconitine were 98.79%,100.66%,97.06%,97.91%,103.58% and 102.77% respectively with RSD of 1.90%,2.23%,1.91%,1.96%,1.08% and 2.19% respectively. Conclusion: The method is simple,reproducible and accurate;it can be used as the quality control methods of the Chinese medicine processed Aconitum carmichaeli.关键词：processed Aconitum carmichaeli;ester-type alkaloids;quality standard;thin-layer chromatography;high performance liquid chromatography
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical composition of the flower volatile oil of wild Vitex negundo L. var. heterophylla from Shijiazhuang. Method: The volatile oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Result: Twenty constituents representing 68.6% of the total volatile oil were identified. Conclusion: The major components of the volatile oil were β-caryophyllene (30.6% ),β-phellandrene (8.4% ) and eucalyptol (1,8-cineole) (7.5% ). The volatile oil can serve as a good source of β-caryophyllene.关键词：Vitex negundo var. heterophylla;Verbenaceae;volatile oil;GC-MS;β-caryophyllene
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发布时间：2024-01-04 - 摘要：Objective: To study the integrated pharmacokinetics of Huanglian Jiedu Tang active fraction(HLJDTAF) in middle cerebral artery occlusion(MCAO) rats. Method: MCAO model of rats was established by electrocoagulation,after oral administration with HLJDTAF,the blood samples in sham-operated and model group were collected at different time points. The plasma concentration of four component was determined;Combined with the time-concentration curve,the plasma concentration of components in sham-operated and model group was compared after oral administration. Result: Compared to the sham-operated group, area under the plasma concentration time curve(AUC)(0-t), Cmaxof model group was higher and mean retention time(MRT)(0-t), t1/2z was short. HLJDTAF hada better absorption and a higher peak concentration in MCAO rats;at the same time,HLJDTAF accelerated the metabolism and had a shorter residence time in the body. Conclusion: The integration pharmacokinetic characteristics are similar to pharmacokinetic characteristics of a single ingredient in HLJDTAF. The obtained parameters could express the whole characteristics of Chinese herbal compound.关键词：Huanglian Jiedu Tang;Active fraction;anti-cerebral ischemia;integrated pharmacokinetics
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发布时间：2024-01-04 - 摘要：Objective: To examine the effects of Xuefu Zhuyu decoction (XFZYD) on the expression of cytochrome P450 (CYPs) genes,glutathione S-transferase (GSTs) genes and UDP-glucuronosyl transferase (UGTs) genes in liver tissues of male Sprague-Dawley rats. Method: Twenty-five rats were divided randomly into 5 groups,5 animals each. Three active dose groups,which were administered with XFZYD by gastrogarage for 15 days at the dose of 3.51,7.02 and 14.04 g· kg-1,respectively. The control group and positive group received the same volume of water per kg body weight. From 13th day,the positive group received Phenobarbital sodium at the dose of 80 mg· kg-1 by peritomeal injection for 3 days. About 200 mg livers were removed,the levels of genes expression of CYP1A1,CYP1A2,CYP2B1,CYP2E1,CYP2C11,CYP3A1,CYP3A2,CYP4A1,CYP7A1,CYP17A1,CYP27A1,GSTA2,GSTM1,GSTp1,UGT1A1 and UGT2B1 in liver tissues of rats were examined by Quantitative real-time reverse-transcription polymerase chain reaction (quantitative real time RT-PCR) assays. Result: Compared with the contro group l,phenobarbital sodium (the positive group) significantly increased mRNA expressions of CYP2B1,CYP3A1,CYP3A2,GSTA2 and UGT2B1 (84.57-fold,5.44-fold,5.11-fold,7.76-fold,13.27-fold,P<0.01,respectively). CYP1A1 (57.92-fold,P<0.01) and GSTA2 (1.54-fold P<0.05) were dramatically induced by XFZYD at the dose of 7.02, 3.51 g· kg-1,respectively. While CYP2C11 (55%,P<0.05) was significantly inhibited by XFZYD at the dose of 14.04 g· kg-1,a inhibitory tendency was found on expression of GSTM1 (P<0.05) at the dose of 3.51,7.02, 14.04 g· kg-1 (53%,55%,51%,respectively),however,XFZYD had no significant effect on the expression of CYP1A2,CYP2B1,CYP2E1,CYP3A1,CYP3A2,CYP4A1,CYP7A1,CYP17A1,CYP27A1,GSTp1,UGT1A1 and UGT2B1. Conclusion: XFZYD can significantly induced the expression of CYP1A1 and GSTA2 gene,but significantly inhibited CYP2C11 and GSTM1 genes,which indicated that when XFZYD is administrated with the substrate of CYP1A1 and CYP2C11,more attention should be paid on drug interactions,XFZYD may affect detoxication and excretion of toxicants,acarcinogens and other substances,and may play a role in the occurrence of malignant tumor.关键词：Xuefu Zhuyu Decoction;CYPs;GSTs;UGTs;gene expression;liver tissue
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the influence of Veratrum nigrum on Panax gingseng's strengthened body resistance based on uniform design. Method: ICR mice were randomly divided into seven groups,including normal control,hypoimmune model and P. gingseng treatment groups. The hypoimmune mice model was constructed by intraperitoneal injection with cyclophosphamide (45 mg· kg-1,qod×3). In order to choose the dose of P. gingseng,the model mice were administered with different dose P. gingsen,qd,for 7 days.The uniform design method was used by two factors and eleven levels to investigate the thymus index,spleen index,phagocytic index,blood routine examination in hypoimmune mice. Result: P. gingseng(0.5-8.0 g· kg-1) could increase the spleen index,phagocytic index,blood routine obviously in hypoimmune mice,which the. weight,immune organ index,blood routine were decreased. The influence of V. Nigrum on P. gingseng:①Immune organ index:the blend medicine(P. gingseng+ V. nigrum=0.53-8.05 g· kg-1) could not affect the thymus index and spleen index obviously. ② Carbon clearance ability:when the rates lowered 100:1 between P. gingseng and V. nigrum, V. nigrum(0.031-0.13 g· kg-1) could decrease phagocytosis functions of P. gingseng and the effect was changed within the rate and dose of them. When the total dose was 3.08 g· kg-1(P. gingseng:V. nigrum 9:1),V. nigrum showed the most powerful antagonistic action on Gingseng. ③Blood routine examination:when the rates lowered 100:1 between P. gingseng and V. nigrum,V. nigrum(0.034-0.13 g·kg-1) could counteract P. gingseng's raising the WBC,RBC but could not affect P. gingseng's dose-response curve. When the total dose was 4.58,3.48 g· kg-1respectively (P. gingseng:V. nigrum 9:1),V. nigrum showed the most powerful antagonistic action on P. gingseng. V. Nigrum not only could counteract P.Gingseng's raising the Hb but also reduce Gingseng's dose-response curve. Conclusion: In the dosage range of Chinese Pharmacopoeia,V. nigrum could decrease even counteract the effects of P. gingseng's strengthen body resistance obviously,the degree of which changed within the rate and dose of them.关键词：eighteen incompatible pairs;Panax gingseng;Veratrum nigrum;strengthen body resistance
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects and possible mechanisms of EtOAc extract from Gastrodia elata on oxidative damage of induced by H2O2 in PC12 cells. Method: PC12 cells were cultured and exposed to H2O2(50-100 μmol· L-1)for 2 h to establish oxidative damage model. The protective effect of EtOAc extract was observed by morphological identification,colorimetric MTT assay,leakage rate of lactic dehydrogense(LDH),intracellular reactive oxygen species(ROS) level and total superoxide dismutase(T-SOD) activity. Result: Compared with the model group,EtOAc extract from G. elata at 20 mg· L-1 could improve the cell morphology significantly,increase the survival rate of 14.7% (P<0.01);EtOAc extract at 20,40,80 mg· L-1 decrease the LDH release of 22.0%,29.3%,20.2% (P<0.01) from cells into culture medium,raise intracellular T-SOD acivity (P<0.01);EtOAc extract at 80 mg· L-1 decrease the ROS level (P<0.01). Conclusion: EtOAc extract from G. elata has a obvious antioxidant effect in vitro.关键词：EtOAc extract from Gastrodia elata;oxidative damage;hydrogen peroxide;PC12 cells
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发布时间：2024-01-04 - 摘要：Objective: To study the preventive effect and mechanism of Xiaoyao San on rat acute liver injury induced by Tripterygium wilfordii. Method: Sixty rats were randomly divided into control group, T. wilfordii model group, ganlixin group, low-dose, medium-dose and high-dose groups of Xiaoyao San. Each group was respectively fed with the corresponding dose of drug for 4 days and then fed with decoction of T. wilfordii for 4 days to make the model except control group. Liver indexes was calculated, alanine aminotransferase(ALT), aspartate aminotransferase(AST), interleukin-1β(IL-1β), tumor necrosis factor(TNF)-αwere measured, and liver pathology was detected. Results: Compared with control group, liver indexes, ALT, AST, IL-1β and TNF-α in model group were significantly increased, and significant pathological changes of liver tissue were found(P<0.05 or P<0.01). Compared with model group, all doses group of Xiaoyao San and ganlixin group could effectively decrease liver indexes, ALT, AST, IL-1β, TNF-α and pathological changes of liver tissue (P<0.05 or P<0.01). Compared with ganlixin group,liver indexes, ALT, AST, IL-1β, TNF-α and pathological changes of liver tissue of all doses group of Xiaoyao San was not significantly different. Conclusion: T. wilfordii-induced acute liver injury is associated with inflammatory injury via IL-1β and TNF-α. Xiaoyao San can protect the liver injury induced by T. wilfordii by inhibiting inflammatory injury.关键词：Tripterygium wilfordii;Xiaoyao San;liver injury;preventive effect
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Yishen Hugu decoction on the changes of the blood and urine biochemical levels,the bone structure,bone biomechanical properties in the rat disused osteoporosis. Method: Seventy SD rats were randomly divided into 7 groups:normal group,sham operation group,model group,high dose,medium dose and low dose of Yishen Hugu decoction group (52,26,16.25 g·kg-1)and positive control group (Drynaria fortunei 0.25 g· kg-1) with 10 rats in each group.The rats in the model group,high,medium,low dose group of Yishen Hugu decoction and positive control group were performed sciatic neurotomy.Normal group was given regular food and water other groups were gavaged Yishen Hugu decoction respectively,and the rats in positive control group were gavaged Drynaria fortunei. The blood and urine biochemical levels were measured,the femora biomechanical test and the tibia were decalcified and then stained with HE and Masson stain. Result: The differences of alkaline phosphatase(ALP),osteocalcin(BGP),tartrate-resistant acid phosphatase(TRACP),hydroxyproline (HYP)/creatinine(Cr),bone mineral density(BMD) and the numbers of nodes and free ends in model group and sham operation group were significant statically (P<0.01). After administration of Yishen Hugu decoction,those indexes in high,medium,low dose group of Yishen Hugu decoction were obviously higher or lower than that in model group (P<0.01). Conclusion: The disused osteoporosis can be prevented effectively by the Yishen Hugu decoction.关键词：disused osteoporosis;animal model;bone metabolism;biomechanical;Yishen Hugu decoction
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发布时间：2024-01-04 - 摘要：Objective: To study inhibitory activity the different separation parts of Ferula sinkiangensis resin on HCT116 human colon cancer cell and determine its effective active site. Method: The effects of the different separate parts of F. sinkiangensis resin (petroleum ether parts,ethyl acetate parts,methanol parts) on colon cancer cells HCT116 proliferation and apoptosis were observed, IC50 and total apoptosis rate were used toevaluate tumor suppression activity performance by SRB and flow cytometry with cell concentration 1×105/mL, and drug concentration of 250,125,62.5,31.25,15.6 mg· L-1 (flow cytometry drug concentration was 62.5 mg· L-1). Result: IC50 of petroleum ether unit was 68.7 mg· L-1,the total apoptosis rate was (43.4±1.1)%;IC50 of ethyl parts:43.7 mg· L-1,total apoptosis rate was (56.2±0.9)%;IC50methanol Department of 59.6 mg· L-1,total apoptosis rate was (46.7±3.1)%. Conclusion: Ferula resin ethanol extract ethyl acetate fraction can promote their apoptosis,initially identified as anti-tumor activity in F.sinkiangensis resin parts.关键词：Ferula sinkiangensis;HCTT116 colon cancer cells;SRB;apoptosis;anti-tumor activity
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发布时间：2024-01-04 - 摘要：Objective: To explore the dose-effect relationship of Maxin Shigan decoction with dosage changes of Gypsum Fibrosum. Method: Based on the original dose of Maxing Shigan decoction,the dose of Ephedrae Herba, Armeniacae Semen Amarum and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle was unchanged,dosage of gypsum was changed,in another Maxing Shigan decoction there was no Gypsum. The male Sprague-Dawley rats were randomly divided in to 13 groups,including Maxing Shigan decoction dose 1-dose 10,positive drug group,normal group and model group. Fever model was induced by injecting lippolysaccharide (LPS) through the tail vein.The temperatures was recorded,and TRI was calculate;concentration of prostaglandin E2(PGE2) in the plasma was detected;Graphpad prism 5.0 software was used to dose-effect analysis. Result: Both the Maxing Shigan decoction with change of dosage of Gypsum Fibrosum and the decoction with no Gypsum could reduce body temperature in fever rats,and reduce the plasma PGE2 levels. Compared to the decoction without Gypsum Fibrosum,the groups with Gypsum Fibrosum significantly decrease body temperature,PGE2 levels and temperature reaction index(P<0.05,P<0.01). Concentration for 50% of maximum effect(EC50) was 2.785 g· kg-1;Therapeutic window for [D]20-[D]80 was 2.218 to 3.497 g· kg-1. Conclusion: Decoction without Gypsum Fibrosum has weak antipyretic effect,which may be related to the effect of Glycyrrhizin,Amygdalin and Ephedrae Herba. Decoction with Gypsum Fibrasum enhanced rapidly antipyretic effect,and with a small dosage range the effect showed a obvious change.关键词：Maxing Shigan decoction;Gypsum Fibrasum;dose-effect relationship;TRI;PGE2
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of flavones of buckwheat (FB) on blood glucose and lipids level,insulin resistance and inflammation factors in Rats with Type 2 Diabetes. Method: Male SD rats were fed high-fat diet after 4 weeks,then injected small dose streptozotocin (STZ) to establish animal models of type 2 diabetes. The rats were divided into 6 groups:normol group,model group,rosiglitazone group(RSg,1 mg·kg-1,ig),low,middle and high dosage-FB group (100,200,400 mg· kg-1,ig). After 4 weeks' treatment,the levels of FBG,TC,TG,LDL-C,HDL-C,FINS,TNF-α,IL-6,CRP were detected,and insulin sensitive index (ISI) was calculated. Result: Compared with the normol group,levels of serum FBG,TC,TG,LDL-C,FINS,TNF-α,IL-6 and CRP in model group were increased(P<0.01),but the levels of HDL-C and ISI were decreased significantly(P<0.01). In FB groups,levels of FBG,TC,TG,LDL-C,FINS,TNF-α,IL-6 and CRP were reduced compared with model group(P <0.01,P <0.05),while HDL-C and ISI were increased in a dose-dependent manner. Conclusion: FB can effectively improve glucose and lipid disorders,improve insulin resistance possibly by decreasing the serum levels of inflammatory cytokines such as TNF-α,IL-6 and CRP of type2 diabetes mellitus rats.关键词：flavones of buckwheat;type2 diabetes mellitus;inflammatory cytokines;insulin resistance;rats
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发布时间：2024-01-04 - 摘要：Objective: To investigate anti-proliferative effects of matrine on human neuroblastoma SK-N-SH cells and its mechanism. Method: Effects of matrine(0.5,1.0,2.0 g· L-1) on SK-N-SH cell proliferation were tested by SRB assay. Cell cycle and apoptosis of SK-N-SH were measured by flow cytometry. The levels of caspase-3 and caspase-9 within the cells were analyzed by Western blot. Result: Matrine inhibited the proliferation of SK-N-SH cells in a dose-and time-dependent manner.The inhibition rate was(19.06±3.17)%,(30.18±3.02)%,(38.55±6.12)%;(45.12±4.02)%,(60.45±5.51)%,(71.38±7.91)%;(58.91±4.36)%,(73.44±8.17)%,(88.37±4.57)% after treated with matrine by 0.5,1.0,2.0 g· L-1 in 24 h,48 h and 72 h,respectively. Flow cytometry apoptosis in the control group and each concentration group were (2.45±0.49)%,(12.56±2.21)%,(19.44±4.32)%,(30.12±3.35)%,compared with the control group,the matrine-treated cells showed increased apoptosis rate (P<0.001),increased percentage arrested in G0/G1 phase (P<0.01) and decreased S phase cells (P<0.01).Matrine significantly increased the expression of caspase-3 and caspase-9 of SK-N-SH cells. Conclusion: Matrine can inhibit the proliferation of SK-N-SH cell and the mechanism may be correlated to cell cycle arrest and apoptosis induced by activation of caspase-3 and caspase-9.关键词：matrine;neuroblastoma;apoptosis;caspase
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发布时间：2024-01-04 - 摘要：Objective: To investigate the apoptosis in human hepatic cancer HepG2 cell strain induced by celosin A(CA) from Celosia Semen and its mechanism. Method: The viability of HepG2 cells was detected by the method cell counting kit-8 (CCK-8).The activity of lactic dehydrogenase (LDH) was measured by thiazolyl blue(MTT)assay.Immune colorimetry was used to test the proliferation of HepG2 cells.The change of morphoiogy of apoptosis HepG2 cells was observed by 4,6-2 amidine base-2-phenyl indole(DAPI) fluorescence staining.Western blot was used to detect the expressions of Caspase-3/7 and nuclear factor kappa B(NF-κB) proteins of HepG2 cells. Result: After HepG2 cells were treated by CA of different concentration,the leakage of LDH increased in a dosage and time dependent manner.In the process of determination of BrdU and CCK-8,the inhibitory effect of CA to HepG2 cell was stronger than standard drugs,and showed clear inhibitory effect on the HepG2 cell proliferation with the increase of concentration and the acting time.By using the DAPI fluorescence dyes,the HepG2 cell cytoplasm concentrating,nucleolus dispersion and chromatin aglomeration were observed.The Western blot analysis showed that the expression of Caspase-3/7 was significantly enhanced and the expression of NF-κB proteins was down-regulated. Conclusion: Celosin A could induce the apoptosis of HepG2 cells and its mechanism related to activating the activity of Caspase-3/7 and down-regulating the expression of NF-κB protein.关键词：Celosia Semen;celosin A;HepG2 cells;apoptosis;NF-κB
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antidepressant effect of Xiaoyao Shuxin capsule (XYSX) and its possible mechanism. Method: Sixty ICR mice were randomly divided into six groups:normal control group,model group,Xiaoyao Wan (1.5 g· kg-1) group and XYSX(0.5,1.5,4.5 g· kg-1) group. Except the normal control group,the other groups were treated with the chronic unpredictable mild stress stimulation (CUMS) combined with lonely raising for 35 d. Then the forced swimming test and free-activity test were carried out. The activity of superoxide dismutase(SOD) and the content of malondialdehyde(MDA) in blood serum were measured by the UV-spectrophotometric determination method and the content of serotonin(5-HT),adrenocorticotropic hormone(ACTH) and brain-derived neurotrophic factor(BDNF) in brain were determined by ELISA. Result: XYSX (1.5,4.5 g· kg-1) could reduce the time of immovability of the depressed mice,increase the activity of SOD,reduce the content of MDA in depressed mice,increase the content of 5-HT and BDNF and decrease the content of ACTH in the brain of the depressed mice. XYSX (0.5,1.5,4.5 g· kg-1) could increase the time of free-activity. Conclusion: XYSX has a prominent therapeutic effect on mice model of chronic stress-induced depression and the mechanism might be related with the reduction of the content of monoamine neurotransmitters,the hyperfunction of hypothalamus-pituitary-adrenal(HPA),the improvement of the neural plasticity and free radical damage.关键词：Xiaoyao Shuxin capsule;depression;monoamine neurotransmitters;HPA axis;BDNF;free radical damage
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发布时间：2024-01-04 - 摘要：Objective: To characterize vasodilatative effect of magnolin (Mag) on isolated rat thoracic aorta,elucidate its possible action mechanism,and its toxicity in rat renal cells NRK in vitro. Method: The thoracic aorta was isolated from male Sprague-Dawley (SD) rats and the isometric tension of aortic rings induced by phenylephrine (PE,1 μmol· L-1) were measured. To investigate the vasodilation effect of Mag on it were observed in the rings with endothelium intact or endothelium denuded. The rat renal cells NRK were cultured in vitro and MTT assay. Result: Mag (0.1-1 000 μmol· L-1) produced concentration-dependent response,relaxations in PE-contracted aortic rings with endothelium. The half maximal effective concentration (EC50) of Mag was 24.43 μmol· L-1. The vasodilatative effect by Mag was not statistically inhibited by 100 μmol· L-1 (L-NAME) in the preparations with endothelium. The relative growth rates (RGR) of Mag (0.1-1 000 μmol· L-1) were between 84.17% and 134.60%. Conclusion: Magnolin causes relaxation of aortic ring through endothelium-dependent pathway. The mechanisms might be involved in eNOS. Magnolin is safe to rat renal cells NRK with concentration between 0.1 μmol· L-1and 1 000 μmol· L-1.关键词：vasodilatation;magnolin;endothelial nitric oxide synthesis;cytotoxicity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Sanhuangfang compound on lipopolysaccharide (LPS)-induced production of inflammatory cytokine in RAW264.7 cell. Method: The cytotoxicity of Rheum palmatum,Scutellaria baicalensis,Phellodendron chinense and Sanhuangfang compound on RAW264.7 cells was detected by CCK8 method;Levels of nitric oxide(NO),interleukin(IL )-1β,prostaglandin E2(PGE2) and tumor necrosis factor-α(TNF-α) in supernatant were assayed by ELISA. Result: Sanhuangfang compound inhibited the secretion of NO,IL-1β,PGE2 and TNF-α in LPS-induced RAW264.7 cells. Conclusion: Rheum palmatum,Scutellaria baicalensis,Phellodendri chinensis and Sanhuangfang compound in 100-1.562 5 mg·L-1 concentration has non-cytotoxic macrophages. The anti-inflammatory effects of Sanhuangfang compound may be mediated in part by inhibiting the production of inflammatory mediators.关键词：Rheum palmatum;Scutellaria baicalensis;Phellodendron chinense;Sanhuangfang compound;inflammatory cytokine
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Sodium Cantharidinate and Vitamin B6 injection (SCV) on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2,and to explore its underlying mechanism. Method: After treatment with different concentrations of SCV (0,1,5,10 mg· L-1) for different periods (12,24,48 h),the proliferation of HepG2 cells was analyzed by MTT. The cell cycle and apoptosis rate were detected by flow cytometry (FCM). The protein levels of protein kinase B(Akt),phosphate Akt(pAkt),Bcl-2 associated x protein(Bax),B cell lymphoma/leuremia-2(Bcl-2) and p21 were detected by Western blot. The mRNA levels of Bax,Bcl-2 and p21 were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: SCV significantly inhibited cell proliferation and induced apoptosis of HepG2 cells. Those effects were in a time and dose-dependent manners. SCV induced an increase in the percentage of G0/G1 phase cells and a decrease in the percentage of S phase cells accompanied by the change in DNA ploidy(P<0.05). The levels of Bax and p21 mRNA expression in HepG2 cells was significantly up-regulated after treatment with SCV (P<0.05),while the expression level of Bcl-2 mRNA was down-regulated. The expression levels of pAkt and Bcl-2 proteins were down-regulated,while that of Bax and p21 protein was up-regulated(P<0.05). Conclusion: SCV could inhibit cell proliferation and induce apoptosis in human hepatocellular carcinoma cell line HepG2.PI3K/Akt signaling pathway and the related downstream targets are involved in those processes.关键词：Sodium Cantharidinate and Vitamin B6;PI3K/Akt signaling pathway;hepatocellular carcinoma;apoptosis;Bcl-2;Bax;p21
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发布时间：2024-01-04 -
Effects of Volatile Oil of Jingfukang Granule on Thrombosis, Blood Viscosity and Cerebral Blood Flow
摘要：Objective: To study the effects of volatile oil of Jingfukang granule (VOJFKG)on thrombosis,blood viscosity,cerebral blood flow (CBF) and cerebral vascular resistance(CVR). Method: The vitro thrombus was induced and the length,wet and dry weight of the thrombus were measured. Thrombosis instrument was used to observe the in vivo occlusion time (OT). At the same time,the platelet aggregation rate,the high-,middle-,low-shear blood viscosity as well as the plasma viscosity in rats were determined.The CBF and CVR were measured before and after drugs in dogs. Result: VOJFKG can delay the OT,decrease the length,wet and dry weight of in vitro thrombus and the platelet aggregation rate,reduce the blood viscosity,increase CBF. Conclusion: VOJFKG can significantly promote blood circulation and remove blood stasis,andimprove the head blood-supply insufficiency.关键词：volatile oil of Jingfukang granule;thrombus;platelet aggregation;whole blood viscosity;plasma viscosity;cerebral blood flow- 2
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发布时间：2024-01-04 - 摘要：Objective: To explore the method of establishing the animal model of coronary heart disease due to heart and spleen Yang deficiency syndrome and observe the effects of Fuzi Lizhong decoction interference. Method: Hundred Wistar rats were randomly divided into control group,model group,Fuzi Lizhong decoction low-dose group,middle-dose group and high-dose group 5 groups with 20 rats in each group. The model group was applied by incising brown adipose tissue(BAT) then with high fatty diet and cold stimulation every other day for 21 days. From the 22nd day,the model group was given subcutaneous injection with pitutrin,once a day for 7 days and from the 29th day,low-dose group,middle-dose group and high-dose group were ig respectively with Fuzi Lizhong decoction (10,20,40 g·kg-1) based on model group.Once a day for 7 days. To observe the changes of the cardiac function,creatine kinase(CK),cholinesterase(CHE),triglycerides(TG),total cholesterol(TC),rectal temperature and weight. Result: The model rats were familiar to the pathologic change of CHD hyperlipoidemia,the myocardial ischemia damage and the cardiac insufficiency and low rectal temperature,weight lose,diarrhea of spleen Yang deficiency syndrome. There was significant difference or high significant difference(P<0.05 or P<0.01),compared with the control group,middle-dose group and high-dose group. Conclusion: It may provide a valuable method for establishment of combined with disease and Zheng animal model by applying multiple methods during the establishment of the animal model of coronary heart disease due to heart and spleen Yang deficiency syndrome. high-dose Fuzi Lizhong decoction could improve the pathologic changes of model rats to some extent.关键词：coronary heart disease;spleen yang deficiency syndrome;animal model;Fuzi Lizhong decoction
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Xingnao Yizhi capsule on learning and memorizing consolidation of dysmnesia mice induced by scopolamine hydrobromide. Method: Sixty mice were divide into normal control group,model group, Huperzine-A group(0.05 mg· kg-1 ),Xingnao Yizhi capsule of high dose group, middle dose group and low dose group(179.2,89.6,44.8 mg· kg-1).After the intragastric administration twice a day respectively for 15 days, the learning and memory consolidation of dysmnesia model was induced by scopolamine hydrobromide intraperitoneal injection(5 mg· kg-1). Learning and memory function of various group rats were assessed with step-through test and step-down test;The content of malondiadehyde(MDA), activity of superoxide dismutase(SOD) in brain tissue were detected. Result: Compared with model group, Xingnao Yizhi capsule(179.2 mg· kg-1)prolonged the latency periods of memory and significantly reduced the error times within 5 mins in dysmnesia groups (P<0.05), increased the activity of SOD and decreased the content of MDA in brain tissue (P<0.05). Conclusion: Xingnao Yizhi capsule has protective effects on learning and memorizing consolidation of dysmnesia mice induced by scopolamine hydrobromide.关键词：Xingnao Yizhi capsule;learning and memorizing;superoxide dismutase;malondiadehyde
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Alpiniae Oxyphyllae Fructus on defecating function of normal mice and its possible mechanism, and the dry effects were characterized. Method: The SPF mice were divided into 5 groups: normal group, Alpiniae Oxyphyllae Fructus with different doses groups (20,10,5 g· kg-1), Alpiniae Oxyphyllae Fructus(20 g· kg-1) and increase liquid soup(20 g·kg-1) combination group. All mice were orally administered once a day for 28 d. In the 7,14,21,28 d to observe the effects of defecation function to animals, and from the aspects of intestinal moisture, colonic electromyography to explain the mechanism that the dry effects of Alpiniae Oxyphyllae Fructus impacting on gut. Result: After 6 h observation, the first time of defecation with low dose was 142.7 min, dry manure was 10.1 grains, weight was 176.6 mg in 28 d; the first time of defecation with moderate dose was 145.4 min, dry manure was 10.2 grains, weight was 177.0 mg in 21 d; the first time of defecation with high dose was 160.3 min, dry manure was 9.7 grains, weight was 170.5 mg in 21 d. Alpiniae Oxyphyllae Fructus is able to make the normal animal appear symptoms of constipation gradually through dose-dependent and time-dependent. After administrated for 28 days, the moisture content of colon lumen of low dose, moderate dose and high dose group of Alpiniae Oxyphyllae Fructus was 66.23%,64.42%,61.53%, significantly reduce the moisture content of colon lumen; frequency of colonic electromyography of low dose, moderate dose and high dose group of Alpiniae Oxyphyllae Fructus was 43.40,45.63,48.26 times per minute, the amplitude of colonic electromyography was 66.23,64.42,61.53 mv, significantly faster frequency of colonic electromyography, reduce the amplitude of colonic electromyography.Increase liquid soup have obvious antagonism effects on above-mentioned constipation caused by Alpiniae Oxyphyllae Fructus. Conclusion: Dry effect characterization of Alpiniae Oxyphyllae Fructus can cause constipation of normal mice, and it's related to the promotion of intestinal water reabsorption and reduced intestinal transit capacity.关键词：Alpiniae Oxyphyllae Fructus;dry property;defecating function;intestinal moisture;colonic electromyography
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of isorhynchophylline on rat great vessels. Method: The rat great vessels were removed after intragastric administration of Uncariae Ramulus Cum Uncis decoction and liquid nitrogen grinding. The tissue samples which were drawn and treated by adding ethanol and the supernatant were dried by N2.After redissolving in carbinol,the supernatant were detected by HPLC directly. The analysis was carried on an analytical column RP18 WatersTM(4.6 mm×250 mm,5 μm). The mobile phase consisted of carbinol-water (70:30),triethylamine to adjust pH 8.0 and the flow rate was 0.8 mL· min-1. The UV detection wave length was at 254 nm and the column temperature was kept at 30℃. The retention time of isorhynchophylline was about 12 min. Result: A good linearity was obtained from 0.04 mg· L-1 to 20.0 mg· L-1 for isorhynchophylline. Regression curve was Y=4.644 1X+0.161 2 with a correlation coefficient (R2) of 0.999 2,the average recovery was 99.34%,and the RSD was less than 8.0%. Conclusion: The method is simple,stable and easy for operation. It is shown to be suitable for determination of isorhynchophylline in rat great vessels.关键词：Uncaria Ramulus Cum Uncis;isorhynchophylline;HPLC;rat;great vessels
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of extracts of Terminalia chebula on animal gastrointestinal activity and serum levels of motilin(MTL). Method: Effects of the extracts of T. chebula on the evacuation of stomach,the propulsive movement of intestine,serum levels of motilin,and the contraction of isolated rabbit intestinal smooth muscle were observed by mice gastrointestinal activity test and isolated rabbit intestinal smooth muscle movement experiment. Result: The extracts of T. chebula could distinctly inhibited the evacuation of stomach and the propulsive movement of intestine in mice,significantly reduce serum levels of motilin,also inhibit the contraction of isolated rabbit intestinal smooth muscle. The extracts of T. chebula showed significant antagonistic effect on spasmodic muscle of duodenum due to acetylcholine chloride. Conclusion: The extracts of T. chebula could inhibit animal gastrointestinal activity,the mechanism of which may be associated with M-receptor.关键词：extracts from Terminalia chebula;evacuation of stomach;propulsive movement of intestine;isolated intestine smooth muscle;motilin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effect and mechanism of saponins of Panax notoginseng (PNS) on drug-induced liver injury in mice. Method: Mice were randomly divided into six groups,control group,model group,low,middle,high-dose (100,200,400 mg· kg-1) of PNS and bifendate group (200 mg· kg-1). The model of drug-induced liver injury was induced by intragastric administration of isoniazid (INH) and rifampin (RFP) in mice. The levels of alanine aminotransferase (ALT),aspartate transaminase (AST) in serum and the content of superoxide dismutase (SOD),malondialdehyde(MDA),glutathione peroxidase (GSH-Px) in liver homogenate were assayed by spectrophotometry. The pathological changes of hepatic tissues were observed by hematoxylin and eosin (HE) staining. Result: The liver index (70.84±5.93),(66.38±4.33) mg· g-1 of in PNS middle,high-dose groups was lower than that (83.18±6.12) mg· g-1in the model group(P<0.05 or P<0.01). Compared with the model group,the levels of ALT (89.71±12.13),(79.58±12.54),(65.86±13.82) U· L-1 and AST (101.54±14.61),(83.70±9.85),(69.47±15.41) U· L-1 in PNS low,middle,high-dose groups were significantly decreased(P<0.05 or P<0.01). The content of MDA (7.80±1.21),(7.07±1.17) nmol· mg-1in PNS middle,high-dose groups was also lower than that (9.62±1.68) nmol· mg-1in the model group(P<0.05 or P<0.01),while the activity of SOD (119.69±14.32),(129.72±20.22) U· mg-1 and GSH-Px (108.02±17.07),(112.72±17.54) U· mg-1 in PNS middle,high-dose groups was higher than that in the model group(P<0.05 or P<0.01). Hepatic tissues stained with HE showed that PNS could remarkably relieve liver injury. Conclusion: PNS has protective effect on drug-induced liver injury in mice,which may be related to antioxidant effect.关键词：saponins of Panax notoginseng;drug-induced liver injury;antioxidant;isoniazid;rifampin
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发布时间：2024-01-04 - 摘要：Objective: To explore the correlations between bone mineral density(BMD) and endogenous hormone levels,and then investigate the mechanism of anti-GIOP of combination of Epimedii Folium and Ligustri Lucidi Fructus. Method: Sixty SD rats were randomly divided into 6 groups:normal control group,model group,Epimedii Folium group,Ligustri Lucidi Fructus group,Epimedii Folium and Ligustri Lucidi Fructus mixture group(compatibity group) and positive drug group. All rats received intramuscular injection of dexamethasone (1 mg· kg-1,twice a week) for 8 weeks to establish osteoporosis model except those in the control group,rats in treatment groups were treated with intragastric administration of traditional Chinese herbs or positive drug respectively,Ligustri Lucidi Fructus group was given Ligustrum lucidum with 9.5 g· kg-1,Epimedii Folium group was given Epimedium with 9.5 g· kg-1, the compatibility group were given Epimedii Folium-Ligust rilucidi Fructus with 9.5 g· kg-1 the positive group was administered Caltrate calcium D3 tablets with 0.277 3 g·kg-1. Eight weeks later, serum corticosteroid (COR),testosterone (T) and estradiol (E2) were determined by radioimmunoassay,and BMD of femur was measured by dual-energy X-ray absorptiometry. Result: The content of serum COR,T,E2 and BMD of modle group were lower than normal control group(P<0.05 or P<0.01). Compared with model group,serum levels of COR were upregulated significantly in each administration group(P<0.05 or P<0.01),serum T levels in compatibility group and E2 levels in Ligustri Lucidi Fructus group and compatibility group were increased significantly(P<0.05 or P<0.01),BMD increased obviously in each treatment group(P<0.05 or P<0.01). The correlation analysis showed that BMD was positively correlated with serum hormone levels(P<0.05 or P<0.01). Conclusion: BMD was closely related to endogenous hormone levels in glucocorticoid-induced osteoporosis rats,which indicate that the effect of combination of Epimedii Folium and Ligustri Lucidi Fructus on hormone levels may be one of the mechanisms of its prevention and treatment of GIOP.关键词：osteoporosis;glucocorticoid;Epimedii Folium-Ligustri Lucidi Fructus;bone mineral density;endogenous hormone;correlation
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effects and mechanism of loureirin B on the dysfunctiion of pancreatic islet β cell induced by palmitic acid. Method: NIT-1 cells were cultured in medium added palmitic acid,and treated with loureirin B. Cell apoptosis ratio and content of reactive oxygen species (ROS) were detected by flow cytometry,the expressions of glucose tnansport receptor-2(GLUT2) was examined by semi-quantitate reverse transcript PCR. Result: Compared with palmitic acid treated group,survival rate of NIT-1 with loureirin B (20,10 μmol· L-1) was increased significantly(P<0.05). Loureirin B (20,10, 5 μmol· L-1) scavenged excessive ROS and lowered its concentration significantly (P<0.05). Loureirin B (20 μmol· L-1) was increased the expressions of GLUT2 (P<0.05),and loureirin B (20,10, 5 μmol· L-1) was effective on reducing apoptosis ratio induced by palmitic acid (P<0.05). Conclusion: Loureirin B can increase the survival rate of NIT-1 cultured in the medium added palmitic acid,scavenge excessive ROS induced by palmitic acid,decrease the cell apoptosis ratio and elevate the expressions of GLUT2.This indicates loureirin B may accomplish its islet cell protection effects by reducing ROS within cell and reversing glucose metabolism disorder rand other effects induced by forkhead box protein-1 (FOXO1) activated by oxidative stress.关键词：loureirin B;insulin-secreting cells;apoptosis;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To investigate the vasodilative effect and the possible mechanisms of aqueous extract of Arctium lappa roots on the thoracic aorta rings of rats. Method: The study was performed with the model of isolate rat thoracic aorta rings in organ bath. Result: Aqueous extract of A. lappa roots did not directly relax or constrict vascular rings. Aqueous extract of A. lappa roots (0.075-15 g· L-1) caused concentration-dependent relaxation with Phenylephrine (PE 5×10-6mol· L-1) preconstricted aorta rings with or without endothelium. The extent of relaxation was larger in endothelium-intact aortic rings than that in endothelium-denuded aortic rings. Relaxation effects were reduced partially but significantly by pretreatment with (ω-nirto-L-arginine methyl ester (L-NAME, 10-4mol· L-1),1H-[1,2,4] oxadiazolo [4,3,a] quinoxalin-1-one (ODQ, 10-5mol· L-1) in endothelium-intact aortic rings. However,they were not affected by indomethacin(10-5mol· L-1). The relaxation response of aqueous extract of A. lappa roots was significantly enhanced by potassium channel inhibitors Tetraethylammonium chloride(TEA). The aqueous extract of A. lappa roots caused concentration-dependent relaxation in 40 mmol· L-1 KCl aorta rings. Conclusion: Aqueous extract of A. lappa roots causes relaxation of aortic rings through endothelium-dependent and independent pathways. The mechanisms might be involved the release of NO from endothelium and is possibly mediated by the activation of Ca2+ channel.关键词：aqueous extract of Arctium lappa roots;thoracic aorta;vasodilative acts;mechanisms
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发布时间：2024-01-04 - 摘要：Objective: To research the effects of Tianshan Huaqiu Pingchuan capsule on relieving cough,dispelling phlegm and anti-inflammation. Method: The establishment of the model control group,Tianshan Huaqiu Pingchuan capsule (1,2,4 g· kg-1) group,positive drug group. Using ammonia induced cough in mice and evaluate tracheal phenol red excretion of Pingchuan capsules antitussive and expectorant function,to observe the frequency of cough and phenol red excretion;using 2% acetylcholine chloride and 0.1% histamine phosphate and volume of mixing liquid induced rat asthma,observe the asthma incubation period of Tianshan Huaqiu Pingchuan capsule on rats with mice;anti-inflammatory effect foot swelling method in evaluation of Tianshan Huaqiu Pingchuan capsule;regulation by measuring the carbon clearance and hemolysin immune evaluation of Tianshan Huaqiu Pingchuan capsules in mice. Result: Tianshan Huaqiu Pingchuan capsule could obviously prolong the latent period and decrease the frequency of cough,promote mouse tracheal phenol red secretion;Rat asthma caused by histamine phosphate has a protective effect,make the rat asthma latency prolonged;and significantly inhibit the swelling degree (P<0.01). In addition,Tianshan Huaqiu Pingchuan capsule can significantly improve the mice carbon clearance index (P<0.05) and the level of serum hemolysin (P<0.01). Conclusion: Tianshan Huaqiu Pingchuan capsule has the apparent significant antitussive,expectorant antiasthmatic,anti-inflammation,and immunological regulative functions.关键词：Tianshan Huaqiu pingchuan capsules;elieving cough;dispelling phlegm;anti-inflammatory;asthma;immunity
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发布时间：2024-01-04 - 摘要：Objective: To provide rationality of Sini decoction compound compatibility based on the toxicity and efficacy. Method: To compare the acute toxicity between the aconite group,Sini decoction group, Aconiti Lateralis Radix Praeparata combined with Glycyrrhizae Radix et Rhizoma group and Aconiti Lateralis Radix Praeparata combined with Zingiberis Rhizoma group,and the median lethal dose (LD50) was determined. To study the effect of the four above mentioned groups the rat heart failure model was induced by adriamycin,and the icarotid artery blood flow,heart rate (HR),left ventricular systolic pressure (LVSP),left ventricular end-diastolic pressure(LVEDP),left ventricular maximum rate of pressure rise(+dp/dtmax) and left ventricular minimum rate of pressure drop (-dp/dtmax)were observed. Result: The result of the acute toxicity experiment showed that in Sini decotion prescription,the attenuation of Aconiti Lateralis Radix Praeparata combined with Glycyrrhizae Radix et Rhizoma group was better than Aconiti Lateralis Radix Praeparata combined with Zingiberis Rhizoma.The experimental results on the effects of Sini decotion showed that Sini decotion group,Aconiti Lateralis Radix Praeparata combined with Glycyrrhizae Radix et Rhizoma group,Aconiti Lateralis Radix Praeparata combined with Zingiberis Rhizoma and the single Aconiti Lateralis Radix Praeparata group had same effects like increasing the LVSP,the+dp/dtmax and the blood output. The effect of Aconiti Lateralis Radix Praeparata combined with Zingiberis Rhizoma was better than Aconiti Lateralis Radix Praeparata combined with Glycyrrhizae Radix et Rhizoma,Zingiberis Rhizoma plays a main role in it. Conclusion: In the prescription compatibility of Sini decocion Glycyrrhizae Radix et Rhizoma plays a main role in the attenuation of toxicity and Zingiberis Rhizoma plays a main role in efficacy,these results illuminate the rationality of Sini decoction compatibility关键词：Sini decotion;compatibility;effectiveness attenuation;toxicity
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to study the clinical curative effect and mechanism action of Sanjie Zhentong capsule in treatment of pelvic inflammatory mass. Method: Ninety-five cases of patients were randomly divided into observation group (48 cases) and control group (47 cases) by digital method. The patients in control group were injected with levofloxacin injection(0.2×100)mL,gtt,2 times/day,metronidazole 200 mL· d-1,course of treatment 10 days. The patients not cured after 10 days could take another course of treatment. Based on the treatment of control group,the patients in observation group took Sanjie Zhentong capsule,1.6 g/time,3 times/day,course of treatment fourteen days. The main clinical symptom scores were recorded,using ultrasound to evaluate the size of package block. Hemodynamic indexes and levels of serum IL-6 and TNF-α were tested before and after treatment. Result: The total effective rate of observation group was 95.7%,superior to 78.7% in the control group (P<0.05). The levels of serum IL-6 and TNF-α of two groups all decreased after treatment,and the observation group was lower than control group (P<0.01). After treatment,the whole blood viscosity (low,medium,high),red blood cells deposition,improvement of cell aggregation index,plasma viscosity of observation group were superior to control group (P<0.01). The lower abdomen pain,lumbosacral pain,aggravation in abdominal pain,increased uterine activity tenderness score,the line thicken and tenderness of attachment region,the size of the enclosed mass in attachment region,the thicken and tenderness of palace skeletons ligament,and total score of observation group were significantly decreased and were lower than the control group (P<0.01). Under ultrasound,the diameter of the enclosed mass was smaller than that of control group,and the difference of reducing of the observation group was better than that of control group (P<0.01). Conclusion: The clinical effect of Sanjie Zhentong capsules is good in the treatment of inflammatory block caused by chronic pelvic inflammation. Its mechanism may improve microcirculation and alleviate inflammatory reaction.关键词：pelvic inflammatory mass;chronic pelvic inflammation;Sanjie Zhentong capsule;blood rheology
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发布时间：2024-01-04 - 摘要：Objective: Breviscapine injection To observe the influence on nerve function and oxidative stress of patients with diabetic peripheral neuropathy disease. Method: Ninety patients were randomly divided into observation group (n=45) and control group (n=45).Patients in the control group were treated with conventional Western therapy. Patients in observation group were treated with mecobalamine tablets,0.5 mg/time,3 times/d,12 weeks as on cycle. Breviscapine injection was added to the patients in observation group,50 mg,1 times/d,2 weeks for one cycle,interval of two weeks after the next course of treatment. Recorded neurological symptoms and signs scores before and after treatment,detected conduction velocity (MNCV) and sensory conduction velocity (SNCV) of the median nerve,ulnar nerve,peroneal nerve,tibial nerve,and detected serum total antioxidant capacity (TAOC),superoxide dismutase (SOD),malondialdehyde (MDA) before and after the treatment. Result: The total efficacy of the observation group (91.11% ) was significantly better than the control group (71.11% )(P<0.05). After treatment,neurological symptoms,signs score and total score were decreased significantly,and the score of observation group was lower than the control group(P<0.01). MNCV of median nerve,ulnar nerve,peroneal nerve,tibial nerve showed significant improvement,the improvement of the observation group was better than the control group (P<0.05 or P<0.01);SNCV of median nerve,ulnar nerve,peroneal nerve,tibial nerve showed significant improvement,the improvement of the observation group was better than the control group (P<0.01);After treatment,TAOC,SOD levels of the observation group were raised and was higher than the control group (P<0.05),MDA levels were significantly decreased,and lower than the control group (P<0.01). Conclusion: Breviscapine injection can improve the patients with DPN on neurological symptoms and signs,and can improve nerve motor nerve conduction velocity and sensory conduction velocity.Curative effect is distinct and its mechanism may reduce oxidative stress injury.关键词：diabetic peripheral neuropathy;Breviscapine injection;nerve function;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: The effects of Lupus 1 number decoction on patients with systemic lupus erythematosus (SLE). Method: Seventy-four patients were randomly divided into observation group (n=37) and control group(n=37). Both groups were given conventional hormone and necessary management. Homemade Lupus 1 number decoction was added to observation group,1 dose each day,conventional decocting with water for 2 times,for 4 months. The main symptom scores of facial erythema,arthralgia,fever were recorded. Dosage of hormone and Systemic Lupus Erythematosus Disease Activity(SLEDAI) scores were recorded. Serum IL-10,serum IL-12,β-arrestinl and sTM level pretherapy and post-treatment were detected. Result: The total effective rate of observation group(100% ) was superior to control group(83.8% ) (P<0.05);after the treatment for 2 months,dosage of hormone of both groups were lower than before(P<0.01);after treatment for 4 months,dosage of hormone of observation group was lower than control group(P<0.01);Observation group of hormone withdrawal reduction than the control group after treatmen(P<0.05);after treatment,the main symptom scores of facial erythema,arthralgia,fever of observation group was lower than control group(P<0.01);after treatment the level of serum IL-10 of observation group has dramatic decline and was lower than control group (P<0.01);after treatment the level of serum IL-12 of observation group was risen and higher than control group(P<0.01);β-arrestinl of observation group was higher than control group (P<0.01);sTM level of observation group was lower than control group(P<0.01). Conclusion: Lupus1 number decoction could effective control the disease activity of patients with SLE,assist the hormone withdrawal of patients and improve the clinical curative effect.关键词：systemic lupus erythematosus;Lupus 1 number decoction;disease activity;hormone withdrawal;β-arrestinl;soluble thrombomodulin
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发布时间：2024-01-04 - 摘要：Objective: The purpose is to observe the clinical curative effect and mode of action of Qufeng Huayu Tongluo Formulae in the early intervention of acute ischemic stroke (CIS). Method: Eiety-five cases of CIS patients were randomly divided into control group (43 cases) and observation group (42 cases) according to digital method. According to 'China Diagnosis and Treatment of Acute Ischemic Stroke Guidelines 2010',patients in both group were given conventional western medicine treatment. Based on the treatment of observation group,patients in the control group took Qufeng Huayu Tongluo Formulae,1 dose/day,treatment course 2 weeks. Nerve function defect severity was assessed by the United States National Institutes of Health Stroke Scale (NIHSS). Limb movement function was assessed by Fugl-Meyer motor function assessment scale (FMI). Limb spasm degree was assessed by modified Ashworth spasm rating scale. Traditional Chinese medicine syndrome scores were recorded.Levels of serum vascular pseudo willebrand factor (vWF),thromboxane B2 (TXB2),6-ketone-Prostcyclin F1a (6-Keto-PG F1a) and homocysteine (HCY) levels and platelet function were detected. Result: On the 7th day and 14th day after treatment,the NIHSS score and modified Ashworth score of observation group were lower than the control group,while FMI score higher than the control group (P<0.01). The falling range of traditional Chinese medicine syndrome scores of observation group was larger than the control group (P<0.01).After treatment,the levels of vWF,TXB2 and HCY of observation group were lower than the control group,while 6-Ketone-PG F1a was higher than the control group (P <0.01). The mean platelet volume and platelet aggregation rate of observation group were lower than control group (P<0.01). Conclusion: In the early intervention of acute ischemic stroke,Qufeng Huayu Tongluo Formulae can improve patient's clinical symptoms of Traditional Chinese Medicine and neural function defect symptom. Its mechanism may be related to inhibit platelet activation and platelet activation,improve the function of vasoconstriction.关键词：acute ischemic stroke;Qufeng Huayu Tongluo Formulae;platelet function;neurological function
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effects of comprehensive treatment of TCM on herpes zoster (HZ)in acute stage and the influence on postherpetic neuralgia. Method: One hundred and twenty patients were randomly divided into observation group (n=60) and control group (n=60). Patients in the two groups were both treated with conventional Western therapy:Famciclovir tablets,0.25 g/time,3times/d,orally;vitamin B1 tablets,10 mg/time,3 times/d,orally;Mecobalamine tablets,0.5 mg/time,3 times/d,orally,Aciclovir ointment Lowe ointment,coated,2 to 3 times/d. Patients in observation group were treated with Chinese medicine therapy:Kangdu Xiaotong Decoction,one dose/d,Cloves Cream,2 to 3 times/d,acupuncture around method,1 times/d,14 days as one cycle. Record herpes blisters stop time,crusting and scab off time;evaluate pain in patients using the Visual Analogue Scale(VAS) method,recording the levels of T cell subsets and serum tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),interleukin-10 (IL-10) before and after treatment,recording the cases of postherpetic neuralgia (PHN) in the followed three months. Result: The total efficacy of the observation group (95.0% ) was significantly better than the control group (83.3% )(P<0.05). crusting and scab off time of the observation group were lower than the control group(P<0.01). VAS scores and lesions of the observed group scores were lower than the control group in 7 and 14 day (P<0.01). Levels of CD3,CD4 and CD4/CD8 were higher than the control group,level of CD8 was lower than the control group(P<0.05 or P<0.01.After treatment,blood TNF-α,IL-6,IL-10 levels of the observed group were significantly lower than the control group than the control group (P<0.01);In the following three months,37 cases occurred PHN in the control group (61.7% ),the observation group was 23 cases (38.3% ),the incidence of PHN of observation group was lower than the control group (P<0.05). Conclusion: Chinese medicine comprehensive therapy can relieve pain in patients with HZ,quickly alleviate skin lesions,herpes promote healing and reduce the incidence of PHN,improve clinical effect;Chinese medicine comprehensive therapy can regulate T cell subsets function,improve immunity,it can also reduce the blood TNF-α,IL-6,IL-10 levels,reduce inflammatory injury.关键词：herpes zoster;Kangdu Xiaotong decoction;Dingxiang extractum;T cell subsets;inflammatory factor
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effect of the Compound Indigoplant oral liquid Solution on children acute upper respiratory infection and evaluate its safety. Method: One hundred and twenty-three children with acute upper respiratory infection were divided into two groups randomly,i e,the treatment group (62 cases,treated with the Compound Indigoplant oral liquid) and control group (61 cases,treated with Ribavirin Spray). Both groups were under conventional therapy. Result: ① The pyretolysis time of treatment group was shorter than control group,(2.8±1.2) d shorter than the control group (3.9±1.1) d,P<0.01.The onset time on pyretolysis of treatment group was earlier than control group,(2.1±1.0) d shorter than the control group (2.9±1.2)d,P<0.01.② The total effective rate on pyretolysis of treatment group 93.5% was higher than control group 85.2%. The total effective rate on pharyngalgia of treatment group 95.2% was higher than control group 80.3%,P<0.05. ③ After treatment for 7 d,the effective rates on symptoms such as cough,nose running and nasal obstruction of treatment group were higher than control group, P<0.05. Conclusion: The Compound Indigoplant oral liquid Solution has rapid onset and high efficacy in treating children with acute upper respiratory infection. Being safe and without any significant side effect,it can be regarded as one of the first choice drugs for children acute upper respiratory infection and has extensive application prospects.关键词：Compound Indigoplant oral liquid;acute upper respiratory infection;children fever
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发布时间：2024-01-04 -
Analysis of Liver Injury in 78 Cases Caused by Rhizoma Dioscoreae Bulbiferae and Related Preparation
摘要：Objective: To analysis the present situation of liver injury caused by Rhizoma Dioscoreae Bulbiferae and related Preparations. Method: The clinical reports about the liver injury collected from medical literature were bibliometrically analyzed. Result: 73.8% of patients cause liver damage after taking Rhizoma Dioscoreae Bulbiferae when doses reach 300-1200 g. Conclusion: Clinical physicians/pharmacists should control the dosage strictly to avoid the drug-induced liver injury.关键词：Rhizoma Dioscoreae Bulbiferae;liver injury;clinical analysis- 3
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Tanreqing injection on patients with high fever of ventilator-associated pneumonia in C reactive protein(CRP) and infection parameters. Method: Fifty-six patients in our hospital of ventilator-associated pneumonia in patients with high fever,according to figures were randomly divided into control group (n=28) and observation group (n=28),the control group with routine treatment including levofloxacin hydrochloride 0.4 g intravenous drip,QD,interferon gamma,subcutaneous injection,1 times/d,L2 weeks after the 1 day;observation group based on the use of Tanreqing injection,1 period of treatment 10 days,changes of two groups were observed before and after treatment of CRP and infection parameters. Result: The observation group and the control group clinical effective rate was 92.86% (26/28),75% (21/28),with significant difference (P<0.05). The observation group before treatment,CRP content was (43.12±0.75) mg· L-1,the content of NT-proBNP is (1 872.99±11.25) ng· L-1;after treatment,CRP content was (11.51±0.22) mg· L-1,the content of NT-proBNP is (673.52±10.71) ng· L-1;the control group before treatment,CRP content was (44.82±0.92) mg· L-1,(NT-proBNP content 1 863.02±11.93) ng· L-1;after treatment,CRP content was (19.64±0.84) mg· L-1,the content of NT-proBNP is (894.12±10.73) ng· L-1;two groups after the treatment,C reactive protein decreased,the observation group decreased than that of the control group (P<0.05);the observation group after treatment (GRAN) for the number of neutrophils (3.33±0.34)×10-9/L,white blood cell (WBC) was (1.52±0.71)×109/L,lymphocyte ratio (LYM) was (51.61±7.64)%;the control group after treatment (GRAN) for the number of neutrophils (5.92±0.15)×109/L,white blood cell (WBC) was (2.92±0.04)×109/L LYM (66.18±6.15)%;compared with before treatment,two groups of neutrophil number (GRAN),with white blood cell (WBC), LYM were decreased significantly (P<0.01),the observation group decreased significantly than that of the control group (P<0.05). Conclusion: Ventilator-associated pneumonia in patients with high fever by phlegm heat quiet drop can effectively inhibit the inflammatory reaction,play an effective antibacterial effect.关键词：Tanreqing;ventilator-associated pneumonia;C reactive protein;infection parameters
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to investigate the relationship between the extent of blood stasis and hypoxia-related proteins in the advanced gastrointestinal cancer patients. Method: We recruited 46 patients with stage IV advanced gastrointestinal cancer,29 patients after radical resection of digestive tract tumor and 19 health adults as control between April 1,2012 and April 10,2013.Fasting venous blood were extracted in hospital the next day,and ELISA test were performed to examine the expression level of vascular endothelial growth factor(VEGF),osteopont in(OPN) and carbonic anhydrase IX(CAIX). Result: The patients with advanced cancer,and postoperative cancer group peripheral blood level of VEGF and OPN,CAIX were significantly higher than that of healthy controls. Between advanced cancer group and postoperative cancer group,the former of VEGF and OPN,CAIX respectively was 1.27 times(P>0.05),1.33 times(P<0.05),1.48 times(P>0.05) of the latter.In the advanced cancer group,the level of VEGF、CAIX in the severe blood stasis group were significantly higher than the mild group. The former respectively was 1.92 times(P<0.01),2.70 times(P<0.01) of the latter.The extent of blood stasis and blood stasis symptoms degree were related to the level of VEGF and OPN,CAIX. The heavier degree of blood stasis,the higher level of anoxia related protein. Conclusion: The expression of hypoxia-related proteins increased gradually with the blood stasis degree in the advanced gastrointestinal cancer patients.关键词：gastroenteric cancer;blood stasis;anoxaemia;vascular endothelial growth factor;osteopontin;carbonic anhydrase IX
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of integration of Chinese and western medicine in treatment of elderly chronic heart failure and the influence for life quality. Method: One hundred and twenty-eight elderly cases with chronic heart failure were randomly divided into control group and experience group. Control group were given western medicine and experience group received integration of Chinese and western medicine treatment. Clinical effect,cardiac function and life quality were compared between two groups. Result: The effective rates of treatment in experience group was much higher than that in control group(90.9% vs 69.4%,P<0.05);left ventricular ejection fraction(LVEF) and left ventriculor diastolic diameter(LVDD) after treatment in experience group were (53.16±2.58)% and (50.31±2.10)mm,of which were superior to control group (P<0.05);The physical,emotion,symptoms and total scores of MLHFQ were (12.02±0.85),(4.67±0.28),(8.09±0.26),(29.31±2.74) in experience group,and were (15.89±1.04),(5.26±0.35),(10.13±0.42),(35.98±3.14) in control group with significant difference(P<0.05). Conclusion: Integration of Chinese and western medicine for elderly chronic heart failure greatly improve clinical effect,cardiac function and life quality.关键词：heart failure;Chinese and western medicine;life quality
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of traditional chinese combined with western medicine in treatment of acne and the influence for negative emotion and life quality. Method: One hundred and twenty eight cases with acne were randomly divided into control group and experience group. Control group received routine western medicine therapy and experience group were given traditional chinese combined with western medicine treatment. Pepression self rating scale(SDS), self assessment scales(SAS) scores and dermatology life quality index(DLQI) scale were used to evaluate negative emotion and life quality. Clinical effect and the change of negative emotion were compared between two groups. Result: The effective rates in experience group was much higher than that in control group (75.8% vs 56.5%,P<0.05);after treatment,SDS and SAS scores in experience group were (34.09±2.21)and (38.13±5.19),of which were much lower than those in control group (38.17±2.64 and 46.80±5.76,P<0.05). The symptoms and feeling,daily activity,leisure entertainment,work and study,interpersonal relationship,therapy and total score of DLQI were(0.98±0.34),(0.63±0.37),(1.01±0.36),(0.62±0.39),(0.85±0.31),(0.78±0.31),(7.21±1.38) in experience group,and (0.98±0.34),(0.63±0.37),(1.01±0.36),(0.62±0.39),(0.85±0.31),(0.78±0.31),(7.21±1.38)in control group (P<0.05). Conclusion: Traditional chinese combined with western medicine for acne greatly improve the clinical effect,negative emotion and life quality.关键词：acne;traditional Chinese;negative emotion;life quality
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发布时间：2024-01-04 - 摘要：Objective: To study the clinical efficacy and safety of elemene injection adjuvant VAD chemotherapy. Method: Multiple myeloma patients were collected and randomLy divided into observation group given elemene injection adjuvant VAD chemotherapy and Vcontrol group given VAD chemotherapy. VCD chemotherapy using intravenous vincristine (0.4 mg),adriamycin (9 mg),oral dexamethasone (40 mg),Elemene injection intravenous drip (0.4 g). Then remission,immune function and adverse reaction were observed. Result: Tcomplete remission rate (38.46% ),partial remission rate (46.25% )and CD3+(68.38±9.03)%,CD4+(47.18±8.49)%,CD4+/CD8+(2.17±0.42),natural killer cells(NK)(9.31±1.34)%,tumor necrosis factor-α(TNF-α)(1.53±0.21)ng· L-1 levels,Improve the stability factor (92.31% ) of observation group were higher than control group;CD8+(21.75±5.68)%,Headache dizziness (23.08% ),gastrointestinal symptoms (7.69% ),renal impairment (7.69% ),liver function damage (7.69% ),heart function damage (15.38% ),bone marrow suppression (7.69% ) were significantly less thancontrol group. Conclusion: Elemene injection adjuvant VAD chemotherapy has positive clinical value for it can improve remission and immune function,reduce adverse reaction.关键词：multiple myeloma;VAD scheme;elemene;clinical remission;immune function
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Jieju Fushenning(JJFSN) in the treatment of early diabetic nephropathy. Method: One hundred and twenty patients with early type Ⅱ DN admitted to our hospital were randomly divided into JJFSN group,Irbesartan group,JJFSN and Irbesartan group,and control group,each group had 30 patients,with 12 weeks as a treatment course.The changes of relevant indicators before and after treatment were observed. Result: In the JJFSN group,the fasting plasma glucose,urinary albumin excretion rate,serum creatinine,glycosylated hemoglobin A1c and other indicators significantly improved.Blood angiotensinⅡ,blood connective tissue growth factor(CTGF),urinary angiotensin Ⅱ,urinary CTGF significantly reduced.The combined application of JJFSN with Irbersartan had better clinical effect than that of Irbersartan and JJFSN used respectively(P<0.05). Conclusion: Jieju Fushenning is effective in the treatment of early type Ⅱ DN,can delay disease progression and improve patients quality of life,thereby worthy of clinical application.关键词：Jieju Fushenning;diabetic nephropathy;angiotensinⅡ;connective tissue growth factor
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发布时间：2024-01-04 - 摘要：Objective: To observate the effect of Kangxian Yixin formula in treating patients with dilated cardiomy opathy(DCM). Method: Eighty-five cases were randomly divided into a treatment group(43 cases)treated with the routine western medicine plus Kangxian Yixin formula(one dose daily),a control group(42 cases) treated with the routine western medicine. Then the changes of the syndrome and cardiac fuction were observed after 6 months. Result: The therapeutic effect of cardiac fuction,TCM syndrome and syndrome integral were better in the treatment group than in the control group (P<0.05). Conclusion: Kangxian Yixin foumula can improve the clinical symptoms and promote cardiac function of the patients with DCM.关键词：Kangxian Yixin foumula;dilated cardiomyopathy;clinical effect
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发布时间：2024-01-04 - 摘要：Objective: To explore the curative effect of Qufeng Zhike decoction in the treatment of subacute cough. Method: According to the random number table,104 cases of subacute cough were randomly divided into study group and control group,the patients in the study group using Qufeng Zhike decoction. And the control group was given powerful loquat capsule for treatment,1 tablets each time. This article compared short-term and long-term effect beteen the two group. Result: The patients in the study group of 38 cases were cured,8 cases markedly effective,effective in 4 cases,the total effective rate was 96.15%;while the patients in the control group 14 cases were cured,15 cases markedly effective,effective in 7 cases,the total effective rate was 69.23%;the statistical analysis found the cure rate (73.08% ) and the total effective rate (96.15% ) of study group was significantly higher that of in the control group (26.92%,69.23% ),and the difference was statistically significant (P<0.05). The average cough symptoms disappeared time of study group [(2.10±1.52) weeks] was shortened than shortened that of control group [(3.52±1.65) weeks] (t=4.11,P<0.05). In addition,to evaluate the long-term efficacy between the two groups found that patients in the study group of 28 cases were cured,effective in 18 cases,the total effective rate was 88.46%;while the patients in the control group cured 20 cases,effective in 12 cases,the total effective rate was 61.54%;the statistical analysis found that long-term study group were significantly higher than the total effective the control group,and the difference was statistically significant (χ2=10.05,P<0.05). Conclusion: The clinical efficacy of Qufeng Zhike decoction is precise,and it can alleviate the pain of patients,and no obvious adverse reaction.关键词：subacute cough;Qufeng Zhike decoction;powerful loquat capsule
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发布时间：2024-01-04 - 摘要：Hotmelt extrusion(HME)preparation formulation was made up of active ingredients,functional excipients and so on,HME technology had several advantages over traditional pharmaceutical technology including absence of solvents,few processing steps,continuous operation and improved bioavailability.This article reviewed pharmaceutical applications of HME,including formulation composition,equipment,preparation process,quality assessment and its solubilization,controlled release, taste masking,in order to provide a reference for promotion of HME in field of Chinese materia medica.关键词：hot melt extrusion;carriers;plasticizers;solubilization;sustained release systems;taste masking
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发布时间：2024-01-04 - 摘要：The Erlong Zuoci pill(EZP) is first documented in the bookwritten by Linghuan in Qing dynasty and embodied byin 2010.As a famous classical prescription of traditional Chinese medicine for the treatment of tinnitus and deafness,EZP has been used to treat sensorineural deafness for various causes.According to literature review,this article systematically discusses the literature records,clinical observations,quality evaluation and pharmacological research of EZP,finds the problems in the research of EZP,in order to provide evidence and ideas for the systematic research of mechanisms and indications.关键词：Erlong Zuoci pill;deficiency of the kidney;deafness
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发布时间：2024-01-04 - 摘要：The pharmacological activities, mechanism and toxicity of research literature about Protocatechuic aldehyde was introduced to provide a reference for reasonable application and quality evaluation of new drug development contained in Salvia miltiorrhiza and protocatechuic aldehyde over the past thirty years. Protocatechuic aldehyde, one degradation compound of Salvianolic acid B found in the roots of S. miltiorrhiza, has an extensive pharmacological activities such as antiatherosclerosis, cardiomyocyte protection, antithrombus, neuroprotection and et al. Its mechanism have been investigated gradually. But Protocatechuic aldehyde is toxic to heart according to some tests. Prescription including S. miltiorrhiza of New drugs about cardiocerebral vascular disease is one of hotspot. therefore, it should be paid attention to process optimization of extraction and drug standard improving the efficacy in case of toxic and side effect.关键词：protocatechuic aldehyde;pharmacological activities;mechanism;toxicity;Salvia miltiorrhiza
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