最新刊期
卷 17 ， 期 16 ， 2011
- 摘要：Objective: To investigate the feasibility of extracting the fat-soluble compounds from Magnolia officinalis with O/W microemulsion. Method: To measure the particle size of blank microemulsion by dynamic light scattering when it is being heated and compare with its physical index. To choose O/W microemulsion, water and ethanol as solvents to extract M. officinalis and determine the content of magnolol and honokiol in extracts of M. officinalis by HPLC. Compared with efficiency of extracting M. officinalis by using the different media, different formulations of O/W microemulsion and using different extraction methods and the differences of M. officinalis’ extracts by TLC. Result: The extracting yield of magnolol and honokiol are more than 90% by using microemulsion at mean time, which is similar to 60% ethanol. The formulations of microemulsion and extracting methods have remarkable effect for extracting the fat-soluble compounds from M. officinalis. There is no significant difference for blank microemulsion pre and post heated. Conclution: It is feasible to extract M. officinalis by using O/W microemulsion as a solvent. It could not only ensure the extracting efficiency of fat-soluble compounds, but also avoid the usage of organic solvents, save energy and reduce the links of manufacture, which are beneficial to the sustained development and low-carbon economy of Chinese Materia Medica.关键词：microemulsion;extraction;M. officinalis;magnolol;honokiol
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect on extracs quality of Evodia rutaecarpa(Juss.)Benth.var.bodinieri(Dode)Huang with different drying methods. Method: Study on compared HPLC and TLC of Evodia alkaloids with different drying methods. Result: The peaks’ type and number of different drying methods extracts was same, slight differences in the content of Evodia alkaloids. Conclusion: The chemical composition and character prints of dried extracts in different ways were equal.Energy-conserved, low-cost methods should be selected as practical application.关键词：different drying methods;Evodia rutaecarpa(Juss.)Benth.var.bodinieri(Dode)Huang;HPLC;TLC;evodia alkaloids
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发布时间：2024-01-04 - 摘要：Objective: Theophylline β-cyclodextrin polymer(β-CDP) microspheres inclusion compound was prepared with β-cyclodextrin as material by coprecipitation method. Method: The preparation process was optimized through the L9(34) orthogonal experimental design and theophylline release from theophylline β-cyclodextrin polymer microspheres was evaluated in vitro.Theophylline β-cyclodextrin polymer microspheres was characterized by laser particle analyzer, FT-IR spectroscopy, thermogravimetric analysis (TGA) and X-ray diffraction (XRD). Result: The optimal procedure was β-CDP microspheres 1 g, theophylline 0.02 g, water 30 mL,time 3 h, and inclusion temperature 60 ℃. Average diameter of optimized products was 162.35 μm and the drug loading and drug encapsulation efficiency were 1.79% and 89.50% respectively. The drug release profile could be described by first-order release equation and Korsmeyer-Peppas equation. Conclusion: The sustained release microspheres had determinate effects be observed and this preparation method was reasonable and feasible.关键词：β-cyclodextrin polymer microspheres;theophylline;regression analysis;drug release in vitro
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发布时间：2024-01-04 - 摘要：Objective: To determine the volatile oil extraction technology of Jinshao capsule and to optimize process parameters. Method: Compared the volatile oils ingredient influence in Jinshao capsule by the way of supercritical CO2 extraction and steam distillation, discussed the problem in industrialized implementation, Optimized parameters of feasible technology. Result: The volatile oil was obtained from Jinshao capsule by Supercritical CO2 extraction. The product stability conforms to the related stipulation. Conclusion: This method is non-pollution, which has high selectivity, also suitable for industrial production.关键词：supercritical CO2 extraction;Jinshao capsule;steam distillation
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction conditions of Xiao’er Fuxie navel patch. Method: Single factor and orthogonal experimental design was used to optimize extracting technology of Xiao’er Fuxie navel patch with the content of rutaecarpine as index. Result: The extraction time of essential oil in the Prescription was 2.5 h; the optimum extraction conditions of Non-volatile active ingredients were reflux extracting 2 times for 1 h each time with 8 times the amount of 70% ethanol. Conclusion: The process validation tests showed that the optimized extraction of Xiao’er Fuxie navel patch was efficient, preferable stability, reasonable and feasible.关键词：Xiao’er Fuxie navel patch;extraction process;orthogonal experiment
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发布时间：2024-01-04 - 摘要：Objective: To study the difference of the process parameters with the three kinds of Chinese herbs polysaccharides by hot water extraction, and to investigate the possibility of combination extraction by orthogonal experiments. Method: Optimize extraction process of three kinds of polysaccharide respectively by orthogonal test, compare yield and water-solubility of polysaccharide extracted with optimal hot water extraction, alkali extraction and sonic extraction. Result: Optimal extraction processes of Rhizoma Chuanxiong polysaccharide was: the temperature of extraction was at 90 ℃, the ratio of sample to solution was 1∶20, and extraction time was 1 h. Optimal extraction processes of Radix Paeoniae Rubra polysaccharide was the temperature of extraction at 80 ℃, he ratio of sample to solution 1∶20, and extraction time 4 h. Optimal xtraction processes of Cortex Phellodendri Chinensis polysaccharide was extraction temperature 90 ℃, the ratio of sample to solution 1∶10, and extraction time 4 h. At the same time compared with polysaccharides extracted by the other two methods, polysaccharide extracted by hot water extraction has higher yield and solubility. Conclusion: The hot water extraction is more suitable for combination extraction.关键词：RHIZOMA CHUANXIONG;Radix Paeoniae Rubra;Cortex Phellodendri Chinensis;polysaccharide;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To ascertain the best drying method for Shenqi Yiqi Guben tablet. Method: Optimized process respectively with vacuum drying, spray drying and belt drying, inspected the amount of powder, water content, thin layer chromatography, paeoniflorin as the index. Result: The belt drying was the best process. Vacuum of -0.1 MPa, drying temperature 80 ℃, feed rate 5 L, track spead 10 Hz. Conclusion: The optimal drying method is reasonable, the active ingredient is not destroyed, suitable for continuous large-scale production, and can reduce production costs and improve the market competitiveness of this product.关键词：Shenqi Yiqi Guben tablet;belt drying;vacuum drying;spray drying;TLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction process of flavone in Phyllanthus urinaria based on celluase, and provide the basis for the efficient use of P. urinaria. Method: The single factor test was performed to study the effects of the material-liquid ratio, extraction temperature, extraction time and the concentration of cellulase on the yield rate of flavone from P.urinaria. The process of extracting flavone based on cellulose was optimized with orthogonal test. Result: The results showed that the optimized extraction condition of flavone in P.urinaria based on cellulase was as following: the concentration of celluase was 4.25 g·L-1, the extraction temperature was 40 ℃, the material-liquid ratio was 1∶30 and the extraction time was 1.5 h. Under the optimized condition, the extraction ratio of flavone was high of 1.51%. Conclusion: In the present conditions, the extracting efficiency of flavone from P.urinaria based on celluase is better, and it has a promising future in application.关键词：Phyllanthus urinaria;flavone;extraction process;cellulase
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发布时间：2024-01-04 - 摘要：Objective: Response surface analysis methodology(RSM) was used for optimizing the extraction technology of total flavonoids from Hemerocallis citrina. Method: On the basis of single-factor tests, extraction temperature, extraction time, solid-liquid ratio and ethanol concentration were selected as influencing factors during extraction. Box-Behnken design was used to establish mathematical model. Result: The optimum conditions for total flavonoids were extraction time 180 min, extraction temperature 75 ℃, ethanol concentration 90%, solid-liquid ratio 1∶35. The predicted extraction yield of total flavonoids was 0.571%, and the verification 0.581%, with relative error of 1.32%. Conclusion: The optimized extraction technology was stable,feasible and simple.关键词：Hemerocallis citrina;total flavonoids;response surface method
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发布时间：2024-01-04 - 摘要：Objective: To optimize SFE-CO2 technique for Fufang Kuiyang Gao. Method: Using the paste rate, the contents of total anthraquinones, imperatorin and ferulic acid as composite index, the effect of condition including temperature,pressure, extraction time on SFE-CO2 extraction was invesitaged by orthogonal design. Result: The influencing sequence of factors on SFE-CO2 for composite index was temperature>pressure>extraction time, and temperature was the most remarkable factor. The optimized extraction condition was as follows: temperature at 55 ℃, pressure at 30 MPa and extration time 1 h. Conclusion: The optimized extraction technology was stable,feasible and simple.关键词：SFE-CO2;Fufang Kuiyang Gao;composite index;Orthogonal Design
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发布时间：2024-01-04 - 摘要：Objective: The extraction process of Fuyajing effervescent tablet was optimized by central composite design-response surface method. Method: The main influential factors on extraction process included the quantity of ethanol, the concentration of ethanol and extraction time. The extraction conditions were evaluated by the yield of extract and the total content of polydatin and osthole. The response surface methodology was used to optimize the conditions of extraction. Result: The optimum condition of extraction was extracted 2 times for two hours with ten times amount of 60% ethanol. Conclusion: It is simple,convenient and high predictive to optimize the extraction process of Fuyajing effervescent tablet by central composite design-response surface method.关键词：Fuyajing effervescent tablet;central composite design-response surface method;extraction process;polydatin;osthole
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发布时间：2024-01-04 - 摘要：Objective: To optimize the conditions for the percolation extraction processes of Xingnaoling granules. Method: The content of total anthraquinones in Rhei Radix et Rhizome and tanshinoneⅡA and the yield of dry ointment were used as indexes to select the optimal extraction processes with single factor test. Result: The optimal percolation extraction conditions are as follows: the medicinal herbs were crushed into coarse grains and soaked by two folds amount of 70% alcohol for 24 hours, collecting 6 folds of percolation fluid at a rate of 3.0 mL·min-1·kg-1. Conclusion: The optimal percolation extraction technology is efficient with high yields, and the results of verification experiment show that the technology is reproducible and stable.关键词：Xingnaoling Granules;tanshinoneⅡA;total anthraquinones in Rhei Radix et Rhizome;percolation
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发布时间：2024-01-04 - 摘要：Objective: To study the fingerprints of Heishunpian from Sichuan Jiangyou, Shanxi Hanzhong and Yunnan Lijiang by high performance liquid chromatographic and establish the method of standard quality for Radix Aconiti Lateralis Praeparata. Method: HPLC method was used to establish the fingerprints of 22 batches of Heishunpian. Alltimate C18 column (4.6 mm×250 mm, 5 μm) was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL·min-1 and a detection wavelength at 240 nm. The obtained data was analyzed by computer aided similarity evaluation and cluster analysis software. Result: There were 11 common peaks in 22 habitats of Heishunpian. The similarity degree of the products produced in Jiangyou was found to be higher than 0.900. Conclusion: The method is accurate, repeatable and reliable, which can be used for their quality control.关键词：Heisshunpian;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective : To establish the optimal ultrasonic extraction technology of polysaccharides from Polygoni Multiflori Radix Praeparata. Method :Extraction time, extraction times, the ratio of sample to solution, and extraction temperature were set as index for orthogonal test, and Compared with hot water extraction method. Result :The optimal ultrasonic extraction Process of polysaccharides from Polygoni Multiflori Radix Praeparata was extracted 2 times for 50 min each time at 70 ℃ with the ratio of dry material to water was 1 ∶25, the yield of polysaccharides is 20.04%. Compared with water extraction, the extraction time reduced obviously. Conclusion :The optimum ultrasonic extraction process for polysaccharides is rapid, efficient and feasible.关键词：Polygoni Multiflori Radix Praeparata;polysaccharides;ultrasonic extraction;hot water extraction
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发布时间：2024-01-04 - 摘要：Objective: Investigate the triperpenoids of Saussurea deltoidea with antitumor activities. Method: Use various chromatography methods such as silica gel, RP-18 silica gel, Sephadex LH-20 column chromatography, HPLC, et al. Result: Seven compounds were isolated from the title plant. Their structures were elucidated by spectral analysis. 7 triperpenoids were isolated and elucidated as taraxast-20-ene-3β, 30-diol (1), 3β-hydroxy -11-oxo-ursan-12-ene (2), 3β-hydroxy-taraxast-20-ene-30-aldehyde (3), 21α-hydroxy-taraxasterol (4), ursan-9 (11),12-diene-3β-ol (5), ursan-9 (11),12-diene-3β-O-acetate (6), 3β-hydroxy-9 (11), 12-diene-oleanol (7). Conclusion: All the compounds were isolated from S.deltoidea for the first time.关键词：Saussurea deltoidea;triperpenoids;antineoplastic herb
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from Momordica charantia. Method: M. charantia was extracted by alcohol and purified by macroporous adsorptive resin. The isolation and purification were carried out by silica gel and semi-prepared HPLC, the compounds were identified and elucidated by spectral and chemical methods. Result: Six compounds were obtained and identified as charantadiol A(Ⅰ), soya-cereboiside Ⅰ(Ⅱ), Naringin(Ⅲ), (3β,20R,23R)-3-{O-6-deoxy-α-L-mannopyranosyl-(1-2)-O- -6-O-acetyl-β-D-glucopyranosyl oxy}-20,23-dihydroxydammar-24-en-21-oic acid 21,23-lactone(Ⅳ), β-sitosterol (Ⅴ) and daucosterol (Ⅵ). Conclusion: Compound Ⅲ and Compound Ⅳ are obtained from M. charantia for the first time. Compound Ⅳ also firstly isolated from this genus.关键词：Momordica charantia;chemical constituents;structural identification
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发布时间：2024-01-04 - 摘要：Objective: To discuss the feasibility on habitat processes method of Sophora flavescons. Method: The water content of medicinal parts of S.flavescens was determined under the different dry conditions, and then the quantities were evaluated in different pieces by comparing the content of water soluble extraction, matrine and oxymatrine accordance with the 'Chinese Pharmacopoeia’. Result: It is more convenient for processing when the water content of medicinal parts was at 45%-40%. At the same times, the content of extraction and active ingredients were close to traditional pieces. Conclusion: Habitat processing method could not only shorten the soften time in processing, but also save the cost of production to ensure quality of pieces. It is a new processing method for modern scale and standardized production of Chinese herbal pieces, and can be widely used in similar Chinese herbs.关键词：Sophora flavescens;pieces;habitat processing
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发布时间：2024-01-04 - 摘要：Objective: To study the content changes of pulegone in Schizonepetae Herba under decoction process, and to provide a reference for normalizing decoction method of Schizonepetae Herba. Method: With index of pulegone, HPLC was used to determine the content of pulegone in Schizonepetae Herba after decoction time of 5, 10, 20, 30 and 45 minutes under given chromatographic condition. Result: Decoction time could significantly affect the content of pulegone in Schizonepetae Herba, and pulegone could not be detected after decoction time of 30 minutes. Conclusion: The special requirements in traditional decoction methods of herbs should be respected. Herbs containing essential oils should be added later during decoction, and in accordance with clinical experiences, decoction time for Schizonepetae Herba shall be 5 minutes.关键词：Schizonepetae Herba;pulegone;content determination;decoction;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining five flavonoids in Artemisia frigida in order to review the collection time. Method: The content of five flavonoids in different collection time and parts of A. frigida was determined by HPCE and the content was compared to predicate the collection time. Result: The linear ranges of five flavonoids were 1.00-40.00 mg·L-1 (r=0.999 8), 2.00-80.00 mg·L-1 (r=0.999 9), 0.50-80.00 mg·L-1 (r=0.999 6), 1.00-40.00 mg·L-1 (r=0.999 3) and 1.00-40.00 mg·L-1 (r=0.999 4) respectively. The average recoveries of five flavonoids were 99.31% (RSD 1.60%), 98.14% (RSD 1.03%), 98.37% (RSD 1.57%), 97.92% (RSD 0.94%), 98.16% (RSD 1.20%). Conclusion: The content of five flavonoids in the flower is more than that in other parts. The optimal collection time is from the middle of June to the end of July.关键词：Artemisia frigida;HPCE;flavonoids;simultaneous determination
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发布时间：2024-01-04 - 摘要：Objective: To establish the fingerprints of Malus hupehensis by HPLC. Method: Hypersil BDS C18 (4.6 mm×250 mm, 5 μm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was at 25 ℃ and the detection wavelength was at 287 nm.The detection time was 70 min and the flow rate was 1.0 mL·min-1. Result: There were eleven characteristic peaks indicated in fingerprints. Conclusion: This method is simple and accurate with a good reproducibility. It provides a reference standard for the quality control of M. hupehensis.关键词：Malus hupehensis;HPLC;fingerprints
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发布时间：2024-01-04 - 摘要：Objective: To develop a HPLC method for determination of tanshinol, protocatechuic aldehyde, ferulic acid, salvianolic acid B, formononetin in compound preparation of Fuzheng Jiedu Keli. Method: The chromatographic separation was performed on a Dikmal ODS-C18 column (4.6 mm×250 mm, 5 μm) maintaining at 30 ℃ during the whole process. The mobile phase consisted of methanol and 0.05%H3PO4 aqueous solution at a flow rate of 0.8 mL·min- 1. The detection wavelength was set at 280 nm for tanshinol, protocatechuic aldehyde, salvianolic acid B, 316 nm for ferulic acid, 248 nm for formononetin. Result: All the compounds were linear (r>0.999) in the range of the test concentrations, and the average recovery of the method was between 98% and 101%, RSD<3.55%. Conclusion: The method is proved to be credible, sensitive, accurate and repeatable. It can be applied to determine tanshinol, protocatechuic aldehyde, salvianolic acid B, ferulic acid, formononetin in compound preparation of Fuzheng Jiedu Keli simultaneously and provide a basal method of quality control to this preparation and other relative preparations.关键词：tanshinol;protocatechuic aldehyde;salvianolic acid B;ferulic acid;formononetin;HPLC;content determination;Fuzheng Jiedu Keli
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method of hesperidin in Nvjin pills. Method: Hesperidin in Nvjin pills was determined by HPLC, using a Shim-pack VP-ODS column(6.0 mm×150 mm, 5 μm), with a mobile phase as methanol-acetonitrile-0.1% phosphoric acid (12∶13∶75), and a UV detecter at 284 nm wavelength. Result: Hesperidin showed good linearity from 0.02 μg to 4.9 μg (r＝0.999 9). The average recovery of big honeyed pills and water-honeyed pills were 102.0% (RSD 1.3%, n=9) and 96.1% (RSD 1.3%, n=9) respectively. Conclusion: The method was simple, accurate, and with good reproducibility. It can be used well as quality control for Nvjin pills.关键词：Nvjin pills;hesperidin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop a high performance liquid chromatographic (HPLC) method for simultaneous determination of ferulic acid, 5-methoxypsoralen, imperatorin, isoimperatorin in Duliang dropping pills. Method: Duliang dropping pills were extracted supersonically with 75% methanol for 30 min. The supernatant was separated on Agilent Zorbax SB C18 (4.6 mm×150 mm, 5 μm) by gradient program with methanol (A)-water with 0.1% phosphoric acid (B) as the mobile phase at a flow rate of 1.0 mL·min-1, and determined by diode array detector at 254, 320, 330 nm respectively. Result: The limits of quantification of four compounds were in the range of 3-15 mg·kg-1, this method showed a good linearity between 0.5-50 mg·L-1 and the correlation coefficient (R2) was more than 0.999 0. The average recoveries were from 96.6% to 102.5% with relative standard deviation from 0.16% to 2.07%. Conclusion: This method is simple and rapid and suitable for determination of the four compounds in Duliang dropping pills.关键词：HPLC;Duliang dropping pills;ferulic acid;5-methoxypsoralen;Imperatorin;isoimperatorin
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发布时间：2024-01-04 - 摘要：Objective: To study the method of determining luteoloside in Reduping Granules. Method: The content of luteoloside in Reduping Granules was determined on an Thermo Hypeisil GOLD column (4.6 mm×250 mm, 5 μm) eluted with acetonitrile and water containing 0.5% glacial acetic acid as mobile phases in gradient mode. The flow rate was kept at 1.0 mL·min-1, and the column temperature was set at 30 ℃. The UV detector wavelengths were at 350 nm. Result: Luteoloside was linear in the range of 0.034 7-0.694 μg(r=0.999 99). The average recovery was 99.3% and RSD was 0.5%. Conclusion: The established method is accurate and reliable, can be used to control the quality of this preparation effectively.关键词：Reduping Granules;luteoloside;determination;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determination of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xuanfei Zhike Oral Liquid. Method: The separation was performed on an Hedera ODS-2 (4.6 mm×250 mm, 5 μm); the mobile phase consisted of acetonitrile-0.2%phosphoric acid (5:95) with the flow rate of 1 mL·min-1 for the determination of ephedrine hydrochloride, D-pseudo-ephedrine and 0.7 mL·min-1 for amygdalin, UV detection wavelength was at 210 nm and column temperature was 25 ℃. Result: ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin were linear in the range of 0.043 2-0.684 μg (r=0.999 9), 0.048 8-0.976 μg (r=0.999 9) and 0.047 0-0.940 8 μg (r=0.999 8), the average recoveries(n=6) of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin were 96.15%, 96.09% and 95.74% with RSD of 0.78%, 1.18% and 1.80% respectively. Conclusion: This method is rapid and accurate for the simultaneous analysis of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin and can provide a reliable way for the quality control for Xuanfei Zhike Oral Liquid.关键词：HPLC;Xuanfei Zhike Oral Liquid;ephedrine hydrochloride;D-pseudo-ephedrine;amygdalin
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for the content determination of strychnine in Jiegu Cuoshang capsule. Method: HPLC was performed on DIKMA C18 column(4.6 mm×250 mm, 5 μm),the mobile phase consisted of methanol-water-glacial acetic acid-triethylamine (76∶230∶2.4∶0.3)and detecting at 254 nm.The flow rate was 1.20 mL·min-1,and the temperature was 30 ℃. Result: The linear range of strychnine was 0.06-1.20 μg(r=0.999 8). The average recovery was 98.2%, and RSD was 2.2%. Conclusion: The method is simple, feasible, and reproducible, and it can be used for the quality control of Jiegu Cuoshang Capsule.关键词：HPLC;strycnine;Jiegu Cuoshang capsule
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发布时间：2024-01-04 - 摘要：Objective: To analyze the fat-soluble components of Lycopus lucidus. Method: The fat-soluble components were extracted by cold-soak extraction, and GC-MS was used to analyze their components. Result: Twenty compounds were extracted from the L. lucidus . 22 compounds were identified, amounting for 75.19% of the total constituents. Conclusion: The esters were the main components, amounting for 38.67% of the total, followed by sesquiterpene amounting for 26.58%.关键词：Lycopus lucidus;fat-soluble components;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the Herba Ecliptae. Method: The chemical constituents in the Herba Ecliptae extract were isolated and the instructures were identified by physical and spectral analysis. Result: Nine compounds were isolated and identified as wedelolactone (Ⅰ), isodemethylwedelolactone (Ⅱ), 3-oxo-16α-hydroxy-olean-12- en-28- oic acid (Ⅲ), echinocystic acid (Ⅳ), 3,16,21-trihydroxy-olean-12-en-28-oic acid (Ⅴ), ecliptasaponin A(Ⅵ), ecliptal (α-formylterthienyl) (Ⅶ), stigmasterol (Ⅷ), and (-amyrin(Ⅸ), respectively. Conclusion: Compounds(Ⅲ)and (Ⅴ) are isolated from Herba Ecliptae for the first time.关键词：Herba Ecliptae;chemical constituents;structure identification
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发布时间：2024-01-04 - 摘要：Objective: To study chemical constituents of Gardenia jasminoides. Method: Silica gel and Sephadex LH-20 column chromatography were employed for the isolation and purification. The structures were identified on the basis of spectroscopic data and chemical methods. Result: Seven triterpenoids were identified as ursolic acid(1), 19α-hydroxy-3-acetylursolic acid (2), isotaraxerol (3), rotundic acid(4), barbinervic acid (5), clethric acid (6), myrianthic acid (7), respectively. Conclusion: Compounds 2-7 are reported from the genus Gardenia for the first time.关键词：Gardenia jasminoides;chemical constituents;triterpenoids
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发布时间：2024-01-04 - 摘要：Objective: The changes of total flavonoid content, total phenolic content and antioxidant activity in Forsythia suspensa leaves during growth were measured, and the relationship between them was also investigated. Method: The sample powder of F. suspensa leaves was defatted and extracted with 60% methanol by ultrasound-assisted method. The antioxidant abilities were estimated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The total flavonoid content in each extract was investigated by the AlCl3 coloring method, the total phenolic content was investigated by the Folin-ciocalteu method. Statistical comparisons and the correlation between antioxidant component and antioxidant activities were made with the version 13.0 of SPSS soft ware. Result: The results showed that the total flavonoid content, total phenolic content and DPPH radical scavenging activity in leaves were significantly different during growth, the leaves picked in march had the highest the total flavonoid content, total phenolic content, and DPPH radical scavenging capacity. There is a significant positive relationship between flavonoid content, phenolic content and DPPH scavenging capacity, and their correlation coefficients are 0.886 and 0.841, respectively. Conclusion: The F. suspensa leaf on March had the highest DPPH redical scavenging activity, the total flavonoid content and the total phenolic content, this suggest that fresh young leaf can be considered as a valuable sourse of antioxidant products.关键词：Forsythia suspensa;total flavonoids;total phenolics;2,2-diphenyl-1-picrylhydrazyl(DPPH);antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the chloroform portion of Ailanthus altissima. Method: The chloroform portion were isolated and pured by silica gel column chromatography, HP-20 and Sephadex LH-20.Their chemical structures were elucidated by spectral data(1H-NMR,13C-NMR). Result: Five compounds were isolated from the chloroform portion.They were identified as stigmast -4-en-3-one(Ⅰ), stigmast-4,6,8(14), 22- tetra -en-3-one(Ⅱ),5,6,7,8-tetramethoxycoumarin(Ⅲ),ocotillone(Ⅳ),20-hydroxy-dammara-24-en-one(Ⅴ). Conclusion: Compounds Ⅰ,Ⅱ,Ⅲ and Ⅴ were isolated from this plant for the first time.关键词：simaroubaceae;Cortex Ailanthi;chloroform portion;chemical constituent
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of 6-gingerol on expression and transport ability of PepT1 in Caco-2 cells. Method: Caco-2 monolayers were grown on permeable supports. Peptide transport activity was studied using -glycyl-sarcosine ( -Gly-Sar). The densities of PepT1 protein and mRNA (SLC15A1) expression levels were analyzed by Western blot and Real-time quantitative polymerase chain reaction. Result: The total transported Gly-Sar of Caco-2 cells within 60, 120 minutes of group treated by 6-gingerol were higher than those of controlled group, (6.84±0.46), (8.61±0.54) μmol/per well vs (5.62±0.20), (6.54±0.54) μmol/per well, and higher than group of cells treated together with Rp-8-Br-cAMP within 120 minutes, the latter result were (6.92±0.67) μmol/per well. And 6-gingerol showed up-regulated effect on PepT1 protein and mRNA expression, also can be inhabited by Rp-8-Br-cAMP. Conclusion: 6-gingerol has significant effects on promoting the transport ability of dipeptides in Caco-2 cells, which maybe take effects by up-regulating PepT1 protein and mRNA expression.关键词：6-gingerol;peptide transporter 1;transport ability;cyclic adenosine monophosphate
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect and mechanism of Xuefu Zhuyu Decoction inducing endothelial cells migration. Method: Serum pharmacology technique was adopted. Endothelial cells ECV304 were incubated with blank serum or Xuefu Zhuyu Decoction containing serum (XFZYD-CS) at final concentrations of 1.25%, 2.5% and 5%. Cell migration was assessed by wound healing assay. Vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) and nitric oxide(NO) level in the culture supernate were evaluated by ELISA and nitrate reductase method respectively; intracellular NO was studied by fluorescent probe; endothelial nitric oxide synthase (eNOS) activity was assessed and VEGF, bFGF and eNOS expression were evaluated by RT-PCR. Result: 1.25%XFZYD-CS could induce cell migration in the wound healing model, 5%XFZYD-CS could induce cell migration in Transwell, and 2.5%XFZYD-CS could work both methods. All 3 concentrations of XFZYD-CS could elevate bFGF, NO level in the culture supernate, intracellular NO level and eNOS activity. Only 2.5%XFZYD-CS could elevate supernate VEGF level. RT-PCR results showed that all 3 concentrations of XFZYD-CS could upregulate bFGF expression; 2.5% and 5%XFZYD-CS upregulated eNOS transcription; only 2.5%XFZYD-CS elevated VEGF expression, but 1.25%XFZYD-CS was opposite work. Conclusion: Xuefu Zhuyu Decoction could induce endothelial cell migration into angiogenesis via upregulating VEGF, bFGF and eNOS/NO pathways.关键词：Xuefu Zhuyu Decoction;angiogenesis;endothelial cells;migration
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of nourishing kidney and reducing urine by investigating the regulative action of Suoquanwan to HPA. Method: The rat model of kidney deficiency and diuresis was established by adenine (150 mg·kg-1, ig 4 weeks), From the second week after the model establishment, the treatments were ig given, including Suoquanwan (1.170, 0.585, 0.293 g·kg-1), Jinsuo Gujin Wan (0.968 g·kg-1). The contents of CRH, ACTH, cAMP in blood in model group, Suoquanwan group,Jinsuo Gujin Wan group and controll group were determined with ELISA and RIA technology, and also the hypophysis index and adrenal gland index were calculated. Result: Compared with the model group, Suoquanwan could remarkedly increase the contents of CRH,ACTH,cAMP in blood in rats with kidney deficiency and diuresis and improve the hypophysis exponent and adrenal gland exponent. Conclusion: Suoquanwan can enhance the action of body adjusting water metabolize by coordinating the function of HPA.The results of the experiment expound the mechanism of nourishing the kidney and reducing urine of Souquan Wan from the point of view of endocrine.关键词：Suoquanwan;kidney deficiency and diuresis;HPA
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Guizhitang and it’s biotransformation product on catabolizing enzymes of prostaglandin E2(PGE2) in bEnd.3 with IL-1β inducement. Method: Using Elisa and Western blot methods to measure p-p38MAPK,cPLA2,sPLA2,COX-2,PGES,15-PGDH,p-NF-κB and PGE2. Result: Guizhitang and it’s biotransformation product could inhibit the changes in p-p38MAPK,cPLA2,sPLA2,COX-2,PGES,15-PGDH,p-NF-κB and PGE2 in bEnd.3 with IL-1 β inducement. Conclusion: Guizhitang and it’s biotransformation product could inhibit the changing on catabolizing enzymes of PGE2 in bEnd.3 with IL-1β inducement to various degrees. Guizhitang and it’s biotransformation product showed similar effects. It can provide experimental evidence on application development of biotransformation product.关键词：Guizhitang;biotransformation product;rat cerebral microvascular endothelial cells;prostaglandin E2
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发布时间：2024-01-04 - 摘要：Objective: To reveal the mechanism of Zuoguiwan treatment for glucocorticoid induced osteoporosis. Method: Virgin Wistar rats were selected and randomly divided into three groups (n=12-15 per group), including control group, model group and Zuoguiwan group. The rats were injected dexamethasone (1 mg·kg-1, twice a week) for eight weeks to make the model of osteoporosis, and the Zuoguiwan (9.52 g·kg-1·d-1, 6 d per week) was administered intragastrically to the rats in Zuoguiwan group for eight weeks. We analyzed the effect of Zuoguiwan on tibia bone by measuring the relative parameters of the histomorphometric slides of the undecalcified tibia. And the content of bone r-carboxyaluatamic-acid-containing proteins(BGP) and Insulin-like growth factors(IGF- Ⅰ) in serum of rats were detected before and after the treatments. Result: TBV and TFS in model group were decreased significantly, while TRS was increased significantly. And the content of BGP and IGF- Ⅰwere decreased significantly. After being treated by Zuoguiwan, TBV and TFS were increased significantly in Zuoguiwan group, no changes of TRS were observed, while it had the tendency to decrease. The content of BGP and IGF- Ⅰwere increased significantly in Zuoguiwan group. Conclusion: Zuoguiwan can treat glucocorticoid-induced osteoporosis in rats. One of the mechanism is that Zuoguiwan can increase the content of BGP and IGF- Ⅰin serum.关键词：Zuoguiwan;osteoporosis;BGP;IGF- Ⅰ
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutical effect of a Chinese prescription with the function of reinforcing kidney and activating blood on immune premature ovarian failure (POF) in mice. Method: Each female BALB/c mouse was received multipoint immunization of porcine ZP4 (pZP4) in two hind footpads and intra-cutaneously to made the POF model. The mice were randomly divided into three Chinese medicine groups, contorl group, model group and estradiol (E2) group. After administrating Chinese medicine in three different doses (15.0,7.5,3.75 g·kg-1), 0.5% CMC-Na and E2 were given respectively for 20 days, vaginal smearses were visualized microscopically. E2 and pZP4 antibody levels were monitored. The morphology of ovarian tissue inflammation were studied. Result: Compared to the control group, the sex cycles of the high dose group was between 6 and 7 days without apparent change. Compared to the model group, the high dose group developed significantly higher serum E2 level (P<0.01) and lower serum antiboides to pZP4 (P<0.05), and moreover, the number of primary follicles and secondary follicles were increased significantly (P<0.05). Conclusion: The Chinese prescription can improve the ovarian reproductive endocrine function and regulate the developmental capacity of ovarian follicle and corpus luteum in the four cycles. Otherwise, the Chinese medicine might inhibit the autoimmune presenility POF to some extent.关键词：premature ovarian failure;estradiol;zona pellucida antibodies;reproductive endocrine
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Achyranthis Bidentatae Radix and achyranthis bidentatae polysaccharides (ABPS) on the mice’s immune function, and investigate the consistency of both effects. Method: Kunming mice were divided into 5 groups: blank control group, immunosuppression model group, decoction group of Achyranthis Bidentatae Radix, ABPS group and mushroom polysaccharides group. The immunosuppression model of mouse was established with injecting cyclophosphamide abdominally. The effects of the decoction of Achyranthes Bidentatae Radix and ABPS on thymus weight, spleen weight, percentage of phagocytosis, phagocytic index, hemolysin, hemolysis of plaque and lymphocyte transformation rate were compared. Result: The decoction of Achyranthes Bidentatae Radix and ABPS both could improve model mice spleen weight, phagocytic percentage and phagocytic index, promote the formation of hemolysin and hemolytic plaque, and increase the rate of lymphocyte transformation. Compared with model group, both of them could improve immune indexes of the immunosuppressed mouse, and there was significant difference (P<0.01 or P<0.05). With the dosage in this study, the effect of the decoction of herbal pieces at 20 g·kg-1 was equal to that of ABPS at 0.5 g·kg-1 for improving immune function. Conclusion: ABPS is the main effective composition of Achyranthes Bidentata Radix for reinforcing immune function.关键词：Achyranthis Bidentatae Radix;achyranthis bidentatae polysaccharides;immunity
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发布时间：2024-01-04 - 摘要：Objective: To observe effect of Huangqi Gegen decoction on diabetes mellitus and insulin resistance experimentally with models of mouse and rat. Method: Diabetic mouse model was induced by intraperitoneal injection of streptozotocin (STZ,160 mg·kg-1), insulin resistance mouse model was induced by hypodermical injection of hydrocortisone sodium succinate. Type 2 diabetes model with insulin resistance (T2DM-IR) of rat was induced by intraperitoneal injection of lower dosage of STZ (30 mg·kg-1) with high fat diet. The effects of Huangqi Gegen decoction on glucose or lipid metabolism and insulin IR were investigated with these models. Result: Huangqi Gegen decoction obviously improved fasting blood glucose and glucose tolerance of diabetic mice induced by STZ,increased insulin sensitivity in insulin resistance mice,also it showed significant lower fasting blood glucose, improved sugar tolerance, lowered serum hyperinsulinism, increased insulin tolerance, improved ISI and lowered HOMA-IR index, and regulated blood fat, down regulated serum free fatty acids in T2DM-IR rats. Conclusion: Huangqi Gegen decoction can improve glycolipid metabolism, increase insulin sensitivity to cure T2DM-IR.关键词：Huangqi Gegen decoction;diabetes;insulin resistance
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发布时间：2024-01-04 - 摘要：Objective: To observe the regulation effect of angelica sinensis polysaccharides on cytokines of human keratinocyte (HKC) and procollagen secretion of fibroblast cells (FC) in dermis, and to investigate the effect of angelica sinensis polysaccharides in skin wound healing. Method: HKC and FC were cultured in DMEM medium with 10% FBS in an incubator containing 5%CO2 at 37 ℃. And pretreated with extract of Angelica Sinensis polysaccharides, The proliferation of fibroblast cells was measured by monotetrazolium (MTT) colourmetric assay. The expression of transforming growth factor-beta (TGF-β) and interleukin-1 alpha(IL-lα) was evaluated by immunohistochemistry. The effect on fibroblast cells proliferation and collagen type Ⅲ excretion was observed. Result: Angelica sinensis polysaccharides could directly stimulate the HKC’s proliferation effect on FC. Results showed that HKC’s proliferation effect was increased with the concentration of angelica sinensis polysaccharides arising and the peak of HKC’s proliferation effect was obtained with 0.2 g·L-1. The angelica sinensis polysaccharides could regulate excretion of TGF-β and IL-lα in a dose-dependent manner, and proliferation of fibroblast and synthesis of collagen could be enhanced. Conclusion: Angelica sinensis polysaccharides can regulate the expression of TGF-β and IL-lα on keratinocyte and collagen type Ⅲ excretion, so as to stimulate proliferation of fibroblasts. It indicates that human keratinocyte and fibroblast are involved in wound healing. Angelica Sinensis polysaccharides plays an important role in wound healing process.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of carbonized Typhae Pollen on the hemorheological parameters, clotting time and tongue presentations in acute blood-stasis model of rat. Method: The SD rats were divided into control, model, Yunnan White Drug-power, carbo power of Typhae Pollen (high, middle and low dosage, 15,6,3 g·kg-1 accordingly). Except the control group, blood stasis model was established in the rest groups. The hemorheological parameters, the prothrombinase time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB) and the signs of tongue presentations were detected in these groups. Result: Carbonized Typhae Pollen in high, middle and low dosage could decrease the viscosity of whole blood (ηb) at low shear rate, erythrocyte sedime natation rate (ESR) and rigidity of erythrocyte, which increased in model rats. Carbo of Typhae Pollen of high and middle dosage could lengthen TT, PT, shorten APTT and decrease FIB, while low dosage could shorten PT and APTT. Carbo of Typhae Pollen showed therapeutical effect in hydrops and bleeding of tongue and the high dosage showed the best effect. Conclusion: Carbonized Typhae Pollen showed the effect of invigorating blood and hemostasis by ameliorating the abnormal hemorheological parameters and the signs of tongue blood stasis. Carbonized Typhae Pollen in high and middle dosage could lengthen PT and TT, which showed the dosage has two-way regulating functions on hemostasis.关键词：carbonized Typhae Pollen;hemorheology;hemostasis;tongue presentation
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of extract from Munronia henryi Harms(MhH) on anti-inflammation and analgesia and to observe its acute toxicity. Method: Hot-plate procedure and writhing test in mice were adopted in experimental analgesic models,and anti-inflammatory model of mouse ear edema induced by xylene,rat paw edema induced by carrageenin and exudation of abdominal blood capillaries of mice were applied. The median lethal dose(LD50) was determined. Result: MhH (80, 40 mg·kg-1, qd×1) could significantly prolong the pain threshold on hot-plate in mice,reduce the writhing times in mice. It could significantly inhibit the ear edema caused by xylene in mice,rat paw edema induced by carrageenin,and exudation of abdominal blood capillaries in mice caused by acetic acid (80, 40, 20 mg·kg-1, qd×3).The LD50 of MhH was 478.9 mg·kg-1,its 95% confident limit was 446.8-513.2 mg·kg-1. Conclusion: MhH has obvious effect on anti-inflammation and analgesia and its acute toxicity is low.关键词：Munronia henryi;analgesia;anti-inflammation;acute toxicity
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发布时间：2024-01-04 - 摘要：Objective: To find the best processing and abstraction methods for Jiaoai decoction according to its hemostasis action. Method: Female NIH mice were rangomly divided into six groups: control group, four observering groups (four groups of different processed Jiaoai decoction), gongxuening group. The mice were intragastically administered with dose of 0.02 mL·g-1,one time a day, lasted for 7 days. Indexed by bleeding time (BT) and clotting time (CT), the hemostasis actions of different processed Jiaoai decoction were compared in mice. Result: Compared with that of the normal group, BT and CT in the group of fullly processed by wine cooking or that in the partially processed group were significantly shortened. While, there were no significant differences in BT and CT between Jiaoai decoction using raw material totally and the normal group. Conclusion: The hemostasis actions were shown in following descending order: Wine cooking for the totally processed materials > decoction of the totally processed materials > decoction of the partially processed materials > decoction of the total raw materials. The best processing method for Jiaoai decoction is cooking by wine after fully processed.关键词：Jiaoai decoction;processing technique;pharmacological test;bleeding time;clotting time
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of emodin on intestinalmucosa mast cells (IMMC) in mice with intestine ischemia-reperfusion (I/R). Method: Twenty-eight mice were randomly divided into four groups (n=7 each): group A (sham operation group), group B(superior mesenteric artery was clamped for 30 minutes followed by reperfusion), group D1 and D2 (in which emodin 60 or 120 mg·kg-1 was given via gastric tube every day for 3 days before I/R). Intestinalmucosa pathology structure,the ultramicrostructure of IMMC were observed under electron microscope,and tryptase expression was compared for IMMC counting, histamine and tumor necrosis factor (TNF-α) were determined. Result: Compared with that of group A, Chiu’s score and IMMC number, histamine, TNF-α in group B were increased significantly (P<0.05 or P<0.01). Compared with that of group B, IMMC number of group D1, D2 and TNF-α of group D2 were decreased markedly (P<0.05). IMMC ultrastructure in group A was normal, and there was some degranulation phenomenon in group B, such as mast cell envelope coalition and intracellular vacuoles. There was more less intracellular vacuoles in group D1 and D2. Conclusion: Emodin can decrease intestinalmucosa disorganization in mice by inhibiting IMMC activation and degranulation.关键词：emodin;intestinalmucosa mast cells;ischemia-reperfusion;mice
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发布时间：2024-01-04 - 摘要：Objective: To investigate the analgesia and anti-inflammatory effects of the extract of Curcuma kwangsiensis S.G.Lee et C.F.Liang(CKG). Method: Kunming mice (22±2) g were divided into 6 groups randomly. Namely physiological saline group, masculine control group, the extract of CKG low dose (8 g·kg-1) and the extract of CKG high dose (16 g·kg-1) groups. The mice were orally given correspondence drug for 7 days in every group. The analgesia effect of CGK was observed by hot-plate procedure and writhing body method.The anti-inflammatory effects were observed by the pinna swelling model of mice, and the cotton ball granuloma model of mice. Result: The ethanol extract and water extract of CKG could increase pain threshold markedly(P<0.01),the inhibitory rate was 59%, 67%, 59%,76% accordingly. The ethanol extract of CKG high-dose group could inhibite auricle swelling caused by xylenes in mouse(P<0.05),the inhibitory rate was 49%. The ethanol extract and water extract of CKG produced obvious anti-inflammatory effects in other animal models of acute or chronic experimental inflammation(P<0.01). Conclusion: CKG has anti-inflammatory and analgesic effects.关键词：Curcuma kwangsiensis;anti-inflammatory;analgesia
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Xiaoyao Wan (XYW) on the behavior and the content of brain neurotransmitters in mice with chronic restraint stress (CRS), so as to provide some evidence for its clinical application. Method: Mice were restraint for 8 h daily over a period of 10 days to prepare the CRS model. Mice of control and model group were given orally with equal volume of distilled water, and XYW group was given suspension of XYW at the dose of 1.365 g·kg-1 according to its clinical dose 1 h before restraint for 12 days. The locomotor activity of mice was observed in open field test (OFT) on the 11th day and the content of neurotransmitters in various brain regions was examined by enzyme linked immunosorbent assay (ELISA) on the 12th day. Result: Compared with the control group, both ambulation movements and rearing times in OFT were significantly decreased and the content of 5-hydroxytryptamine (5-HT) in hippocampus, norepinephrine (NE) and arginine vasopressin hormone (AVP) in forebrain and histamine in hypothalamus in CRS mice were significantly increased in the model group. XYW could significantly increase ambulation movements and rearing times in OFT and decrease the content of 5-HT, NE, AVP and histamine in brains of CRS mice. Conclusion: XYW could improve not only abnormal behavior but also alteration in content of monoamine neurotransmitters and AVP in brain induced by CRS.关键词：Xiaoyao Wan;chronic restraint stress;behavior;content of neurotransmitters
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of ginseng panaxadiol saponins (PDS) on thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α(6-Keto-PGF1α) in rat model of endotoxic shock. Method: Sixty rats were divided into 6 groups randomly:groups under the names of lipopolysaccharides (LPS),LPS+dexamethasone (DEX, 2 mg·kg-1), LPS+PDS (22.5 mL·kg-1), LPS+PDS (45 mL·kg-1), LPS+PDS (90 mL·kg-1) and control group respectively. PDS was injected into ranine vein ten minutes after septic shock model was induced by injection of LPS (4 mg·kg-1). MAP was measured through carotid arterial cannula. Animals were killed after 4 hours and their livers (20mg each) were taken for homogenate. PGF1α and TXB2 were measured using radioimmunoassay kit. Result: MAP in LPS group was decreased significantly after injection of LPS and maintained at lower level. While MAPs of LPS+DEX, LPS+PDS were not shown obviously decrease. PGF1α and TXB2 of LPS+PDS group were decreased obviously with comparison to LPS group. Conclusion: PDS plays the role of anti-shock through decreasing the levels of PGF1α and TXB2 in rats with septic shock.关键词：lipopolysaccharides;ginseng panaxadiol saponins;thromboxane B2;6-Keto-prostaglandin F1α
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effects of panaxynol against nerve cells injury induced by oxygen glucose deprivation (OGD). Method: Cultured PC12 cells, treated with OGD, panaxynol (10,20 mg·L-1) and nimodipine (100 μmol·L-1), were divided into control group, OGD group, panaxynol group and nimodipine group. Cell morphology was observed by fluorescence microscopy and neuronal apoptosis was detected by Hoechst/PI double-dyed flow cytometry, through to determine the release of LDH and cell i, the neuroprotective effect of panaxynol was observed. Result: Panaxynol could reduce the LDH release of PC12 cell injury induced by OGD (P＜0.05,0.01), restrain the production of Ca2+(P＜0.05,0.01), decrease neuron apoptosis and necrosis significantly (P＜0.05,0.01). Conclusion: Panaxynol can reduce nerve cells injury induced by OGD,has the action for nerve cell protection.关键词：cerebral ischemia;oxygen glucose deprivation;panaxynol;neuroprotective effect
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Wenyang Huazhuo Tongluo recipe (WYHZTLR) on the collagen production and transforming growth factor-beta1(TGF-β1) secretion of systemic sclerosis (SSc) skin fibroblasts in vitro by serum pharmacologic technique. Method: Patients with SSc were treated with prednisone at the dosage of 20 mg·d-1 and D-penicillamine at the dosage of 0.25 g·d-1 or WYHZTLR for one month. Prednisone, D-penicillamine and WYHZTLR contained serum from patients with SSc were respectively added into cultured normal dermal fibroblast cell-line and SSc lesional dermal fibroblast cell-line. Then the cells were incubated for 72 hours. Hydroxyproline and TGF-β1 were examined by colorimetric analysis and ELISA respectively. Result: Different concentrations of drug-contained serum (5%,10% and 20%) of western medicine, Chinese medicine and integrated Chinese and western medicine could markedly decrease collagen production of normal dermal fibroblasts and SSc lesional dermal fibroblasts in vitro compared with control group (P<0.05 or P<0.01). But integrated Chinese and western medicine group could markedly decrease collagen production of fibroblasts compared with western medicine group (P<0.01). Different concentrations of drug-contained serum(5%,10% and 20%) of western medicine, Chinese medicine and integrated Chinese and western medicine could markedly decrease the secretion of TGF-β1 compared with control group (P<0.01). But integrated Chinese and western medicine group could significantly decrease the secretion of TGF-β1 compared with western medicine group (P<0.01). There was a positive linear correlation between the hydroxyproline content and the expression of TGF-β1 protein in SSc lesional dermal fibroblasts of the control group (r=0.895, P<0.01). Conclusion: WYHZTLR can significantly inhibit the secretion of collagen and TGF-β1 of SSc skin fibroblasts, and this maybe one of its pharmacologic mechanism in the treatment of SSc.关键词：systemic sclerosis;Wenyang Huazhuo Tongluo recipe;fibroblast;collagen protein;transforming growth factor-beta1
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Yanshuai mixture (YSM) on the spleen tissue structure and serum interleukin 2 (IL-2) levels in D-galactose induced sub-acute aging mice. Method: The aging mice were established by D-galactose (120 mg·kg-1·d-1) and administered continuously with YSM for 8 weeks. The spleen index and changes in spleen endoscopic biopsy morphology were observed, IL-2 levels in serum were detected using enzyme-linked immunosorbent assay (ELISA). Result: The spleen index of aging model mice was decreased (0.25±0.05)%, histopathological changes in spleen were degraded significantly, and IL-2 level (0.90±0.17)μg·L-1 was significantly reduced compared with those in normal control group (P<0.05). After the treatment with YSM, spleen histomorphological changes were ameliorated, the spleen index of high-dose YSM mice(0.37±0.04) was increased and level of IL-2 in the serum (2.47±0.77)μg·L-1 was increased (P<0.05). High-dose YSM group was superior to Bushen Yishou capsule (BSYS) group, low and middle-dose YSM group (P<0.05). Conclusion: YSM can improve the spleen histopathological changes, inhibit the decline of IL-2 level in serum, and improve immune function, so it may have a good anti-aging effect.关键词：Yanshuai mixture;aging;spleen;interleukin 2
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发布时间：2024-01-04 - 摘要：Objective: To observe promoting blood circulation by removing blood stasis of Huoxue Sanyu granule. Method: Sixty Wistar rats (n=10/group) were randomly divided into six group,the normal goup,the model group, the Fufang Danshen Tablets group, high Huoxue Sanyu granule group(16 g·kg-1),middle Huoxue Sanyu granule group(8 g·kg-1) and low Huoxue Sanyu granule group(4 g·kg-1). The trement groups were orally administered once per day for 9 d separately, whereas the normal and model group were orally administered with distilled water.Except norml rats,all the other rats were injected subcutaneously adrenalin 10 μg·kg-1, swam in icy water 5 min after 2 h the acute blood stasis rat models were made,and these rats were injected subcutaneously adrenalin 10 μg·kg-1 again after 2 h.The indexes on hemorheology after given drugs for nine days were measured order to investigate the influences of Huoxue Sanyu granule;mesenteric microcirculatory disturbance rat models were established with adalin in order to observe the effects of this drug. Result: Huoxue Sanyu granule could decrease rats blood viscosity,plasma viscosity,and relieve viscosity and coagulating condition of blood stasis rats blood,so it inhibited thrombosis;it could also inhibit the caliber decreasing of thin artery,the slowing of blood flow,the reducing of open capillaries and chang of fluid condition and improve these phenomena. Conclusion: Huoxue Sanyu granule may improve blood stasis rats hemorheology and microcirculatory disturbance.关键词：Huoxue Sanyu granule;hemorheology;microcirculatory disturbanc
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发布时间：2024-01-04 - 摘要：Objective: To observe the Pathomorphological changes in rats with pulmonic fibrosis treating by Feiluotong Mistura. Method: Wistar rats were divided into normal group, model group, herbal therapy high dose group, herbal therapy low dose group, western medicine (WM) group. In addition to the normal group, the other four groups were reproduced animal model of pulmonic fibrosis. The model group were given normal saline gavage. The herbal therapy high dose group was given Feiluotong Mistura (18 g·kg-1) gavage. The herbal therapy low dose group was given Feiluotong Mistura (9.0 g·kg-1) gavage. WM group given prednisone gavage. Half of each group were taken lung to test in the 28th day and the 56th day. Result: Large collagen fibers hyperplasia was founded in the model group, a severe pulmonary fibrosis in WM group. Mild pulmonary fibrosis was founded in the herbal therapy high dose group, fibrosis in the herbal therapy low dose group was more severe than that in the herbal therapy high dose group, Lesions of WM group were milder than that of the model group. Conclusion: Feiluotong Mistura can effectively inhibit the formation of pulmonary fibrosis induced by bleocin.关键词：Feiluotong Mistura;pulmonic fibrosis;pathomorphology;experimental study
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Sheng-Yu decoction on peripheral blood cells,bone marrow cell cycle and apoptosis in myelosuppressed mice and to explore its possible mechanism in hematopoietic regulation. Method: The 60 BALB / c mice were randomly divided into blank group, model group, positive medicine group, Sheng-Yu decoction,three dose groups (32,16, 8 g·kg-1·d-1), with 10 mice in each group. Except the blank group. The other 5 groups were established the model of myelosuppression by irradiated 60Coγ and injection of cyclophosphamide and chloramphenicol. After 24 h of the completion of modeling, blank group and model group were ig normal saline, three doses of Sheng-Yu decoction groups were respectively ig corresponding crude drug 32,16, 8 g· kg-1·d-1, with drug volume 20 mL·kg-1·d-1; and the positive control group were injected with rhG-CSF 125 ug· kg-1·d-1. Each group was medicated for 7 days.The effect of Sheng-Yu decoction on peripheral blood cells,bone marrow nucleated cells (BMNCs) and bone marrow cell cycle and apoptosis in myelosuppressed mice was detected by automatic blood cell analyzer,white blood cell counting and flow cytometry (FCM) respectively. Result: Peripheral white blood cells,red blood cells,hemoglobin,blood platelet and BMNCs were increased obviously by Sheng-Yu Granul,groups(P<0.05 or P<0.01). In model group, Sheng-Yu decoction (32,16 g·kg-1·d-1) dose groups, the proportion of G0/G1 phase cells were (70.38±1.38)%, (62.43±2.03)%, (63.85±2.90)%; S phase fraction was (22.52±1.27)%, (26.35±2.52)%, (24.80±1.48)%; G2 / M phase cells (7.43±1.04)%,(11.37±2.18)%,(10.95±2.35)%; PI were (29.85±1.06)%,(37.66±2.01)%,(35.88±3.13)%; apoptotic rates were (26.88±3.58)%,(19.15±4.30)%, (21.65±4.34)% respectively. Compared with the model group, the above parameters of Sheng-Yu Granul,groups were significantly different(P<0.05 or P<0.01). Conclusion: Sheng-Yu decoction could promote erythroid hematopoiesis by accelerating bone marrow cells of proliferation and diferentiation,inhibiting the apoptosis of hemopoietie cells.关键词：Sheng-Yu decoction;myelosuppressed mice;bone marrow cell cycle;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To study the organ targeting for freeze-dried injection of Oxymatrine- Polybutylcyanoacrylate-Nanoparticles (OMAT-PBCA-NP) in rats. Method: The content of oxymatrine in major organs of rats after injected with OMAT-PBCA-NP was investigated by high performance liquid chromatography. HPLC was carried out on a Kromasil C18 column (4.60 mm×250 mm,5 μm)with the mobile phase consisting of methanol and water and triethylamine(50∶50∶0.02), the column temperature was 20 ℃ and UV detection wavelength was 210 nm, the flow was 1 mL·min-1 and the injection volume was 10 μL. Result: The calibration curve of oxymatrine was linear in the concentration range of 50-400 mg·L-1(r=0.999 0),in 30 min,1,4,8 h,the content of oxymatrine was highest in the liver with significant difference from other organs (P＜0.01). Conclusion: OMAT-PBCA-NP has good targeting for liver.关键词：oxymatrine-polybutylcyanoacrylate-nanoparticles;targeting;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the antitussive, expectorant and antibacterial effects of Zhebei Zhike granules. Method: Mice were divided into 5 groups randomly: Normal saline (NS) group, positive group, high dose group, middle dose group and low dose group of Zhebei Zhike granules (24.4,12.2,6.1 g·kg-1), after 6 d by ig administration, the antitussive effect was studied by the cough model induced by ammonia in mice, the expectorant effect was studied by a mouse model of phenol red test. Rats were divided into 5 groups randomly: NS group, positive group, high dose group, middle dose group and low dose group of Zhebei Zhike granules (18.4,9.2,4.6 g·kg-1), after 6 d by ig administration, the expectorant effect was studied by testing the amount of phlegm secreted in rats. Experimental research on antibacterial action of Zhebei Zhike granules was applied to observe the antibacterial effect. Result: The experimental results showed that high dose group and middle dose group of Zhebei Zhike granules could produce a protective effect on the cough induced by ammonia (P<0.01), increase phenol red excretion in mice and phlegm expelling in rats (P<0.05 or P<0.01). The granules showed obvious antibacterial actions against Staphylococcus aureus (the diameter of inhibition zone is 14mm,MIC is 3.9 mg·mL-1,MBC is 7.8 mg·mL-1) and Streptococcus pneumonia (the diameter of inhibition zone is 16mm,MIC is 15.6 mg·mL-1,MBC is 31.2 mg·mL-1),and also showed some protective effect to mice infected with bacteria (P<0.01). Conclusion: Zhebei Zhike granules have obvious antitussive, expectorant and antibacterial actions.关键词：Zhebei Zhike granules;antitussive action;expectorant action;antibacterial action
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发布时间：2024-01-04 - 摘要：Objective: To observe effect of Rhei Radix et Rhigoma on AQP1,5 mRNA expression of alveolar type Ⅱcell(ATⅡ) injured by LPS. Method: Primary cultured alveolar type Ⅱcells were divided into 5 groups: control group: 2mL DMEM cultured medium was added; LPS injured cell model group(acute lung injury cell model group): 10 μg·mL-1 LPS was added to injure AT Ⅱ cell; serum containing Rhei Radix et Rhigoma intervention group was divided into 3 groups: each group was added the total volume of 5%,10%,20% serum containing Rhei Radix et Rhigoma. Which were collected After 4 hours of culture the mRNA expression of AQP1, AQP5 was detected by Real-Time PCR. Result: Compared with the control group, AQP1 mRNA expression of LPS injured cell model group was significantly lower (P<0.05). Compared with the LPS group,20% of total volume of serum containing Rhei Radix et Rhigoma intervention group could increase AQP1 mRNA expression significantly, but not return to the normal level. Compared with the control group, AQP5 mRNA expression of LPS injured cell model group was increased. Rhei Radix et Rhigoma intervention groups could decrease AQP5 mRNA expression, but not significantly. Conclusion: The protective mechanism of Rhei Radix et Rhigoma on acute lung injury cell model by LPS may be associated with the increased AQP1 mRNA expression and the decreased AQP5 mRNA expression.关键词：acute lung injury;alveolar type Ⅱcells;Rhei Radix et Rhigoma;AQP1;AQP5
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发布时间：2024-01-04 - 摘要：Objective: To investigate the inhibitory effect of radicamine B on glucose absorption in rat intestines i n vitro . Method: The inhibitory effect of radicamine B was evaluated by α-glucosidase inhibitory test, inhibitory kinetics assay and assay of glucose absorption in rat intestines in vitro . Result: Radicamine B showed an inhibitory effect on both α-glucosidase and glucose’s absorption in the small intestine in a dose-dependent manner and the IC50 value were 2.19 g·L-1 and 0.094 g·L-1, respectively. No significant difference was found between acarbose and radicamine B in the inhibitory effect on intestinal glucose absorption. In addition, the inhibitory pattern of radicamine B was competitive enzyme inhibition with its Ki of 6.3×10-7 mol·L-1. Conclusion: Radicamine B significantly inhibites glucose absorption in the small intestine. It indecates that radicamine B might be a novel therapeutic drug for diabetes.关键词：radicamine B;α-glucosidase inhibitor;inhibitory effect;acarbose;glucose absorption
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Kaixin Jieyu Decoction on the activity of inducible nitric oxide synthase(iNOS), glutathione peroxidase(GSH-Px) and the content of nitric oxide(NO) in colon tissue of the depressed rats. So as to reveal partial mechanisms of Kaixin Jieyu Decoction for protecting colon of the depression rats. Method: Thirty male SD rats were evenly randomized into normal,model and Kaixin Jieyu Decoction groups, Depression model of rat was produced by using chronic unpredictable mild stress stimulation (forced ice-water swimming,electric shock,tail-clamping,etc) combined with lonely raising for 21 days.At the same time of producing the depression model rats, Kaixin Jieyu Decoction was given to the Kaixin Jieyu Decoction group rats(40g·kg-1), once daily,continuously for 21 days.The activity of iNOS, GSH-Px and the content of NO in colon of the depressed rats were detected by ultraviolet spectrophotometer method. Result: The levels of iNOS and NO were increased significantly and the activity of GSH-Px was reduced significantly in colon of the model rats compared to that of the normal group (P<0.05); also the levels of iNOS and NO were reduced significantly, the activity of GSH-Px was increased significantly in colon of Kaixin Jieyu Decoction group compared to that of the model group (P<0.05). Conclusion: Kaixin Jieyu Decoction had protective effect for colon tissue function of the depressed rats.关键词：depression model;Kaixin Jieyu Decoction;iNOS;GSH-Px;NO
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Huoxiang Zhengqi Liquid on expression of Zounlaoccludens-1(ZO-1) in ileum mucosa of rats with dampness retention syndrome(DRS). Method: Twenty four SD rats were randomly divided into 3 groups: control,DRS group and treatment groups (DRS + Huoxiang Zhengqi Liquid), 8 rats in each group.DRS was induced in all rats,except the control group,by modified enviromental and fatigued method for 6 days.After DRS model was formed,control and DRS groups were lavaged with normal saline(20 mL·kg-1). Rats in treatment groups were lavaged with Huoxiang Zhengqi Liquid(20 mL·kg-1) for 8 days.Then expression of ZO-1 on ileum mucosa was determined by Real-Time PCR. Result: Rats of model group exhibited typical DRS, with sign of hyperaemia, edema, hemorrhagic spots and sludged blood in the gastric mucosa was compared to control. The expression of ZO-1 on ileum mucosa significantly decreased in DRS group compared to that of control group(P<0.01).Conversely, exposure of DRS rats to Huoxiang Zhengqi Liquid resulted in increase in expression in ZO-1 in the ileum mucosa (P<0.01 versus DRS group). Conclusion: Huoxiang Zhengqi Liquid could have the protective effect on DRS-induced gastric mucosal injury, and increasing in expression of ZO-1 may play an important role.关键词：Huoxiang Zhengqi liquid;dampness retention syndrome;ileum mucosa;ZO-1
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the total antioxidant activity of extracts from Polygonum lapathifolium in vitro. Method: 1,1-diphenyl-2-picryl-2-picrylhydrazyl (DPPH) radical scavenging, diammonium salt (ABTS) radical scavenging,and ferric reducing antioxidant power (FRAP) assay were used to analyze the antioxidant activity. Result: Compared with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) and positive control propyl gallate (PG),butylated hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT),the ethyl acetate extract and the methanol extract showed higher scavenging activity against DPPH radical,ABTS radical,and reducing antioxidant power of TPTZ than that of BHT,lower than that of PG and BHA. Conclusion: P. lapathifolium exhibited the good total antioxidant activity. In three extracts of P. lapathifolium,the ethyl acetate extract showed higher total antioxidant activity than that of the methanol extract,and the petroleum ether extract hardly had antioxidant activity.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effect of Bushen Huoxue Decoction on chronic aplastic anemia (CAA) patients, and observe the effect on expression level of myelo-cytokine C-met SF. Method: Sixty CAA cases were randomly divided into treatment group and control group. Treatment group: the patients were given Bushen Huoxue Decoction, and one dose divided into 2 times daily. Control group: the patients were given Zaizhang Shengxue tablet and each took 5 pills 3 times daily. The course of treatment was three months. This experiment employed reverse transcription- polymerase chain reaction (RT-PCR) method to exam the change of expression level of bone marrow mononuclear cell (BMMC) C-met SF receptor mRNA. Result: The expression level of BMMC C-met SF receptor mRNA in CAA patients was higher than that in normal control group. And in both treatment groups the expression levels were descended in different degree. Moreover, the descent in Bushen Huoxue Decoction group was superior to that in Zaizhang Shengxue tablet group. Conclusion: There was abnormal expression of bone marrow SCF receptor C-met SF in CAA patients; Bushen Huoxue Decoction can improve the hematopoietic function of bone marrow to promote restoration by regulating the bone marrow C-met SF mRNA.关键词：Bushen Huoxue Decoction;aplastic anemia;gene;C-met SF
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical effect and safety of western medicine plus linggui Zhugan or added formula versus western medicine alone for chronic heart failure (CHF) patients. Method: We systematically searched the cochrane library, PubMed, EMbase, CBM, CNKI, Wanfang,VIP databases and other relevant databases and journals to identify randomized controlled trials (RCTs). According to evidence-based medicine (Cochrane) systematic evaluation method, we assessed the quality of included studies one by one, extracted valid data and undertook the Meta-analysis with RevMan 5.0.2 software. Result: Four randomized controlled trials involving 280 patients are included.The results of meta-analysis showed that western medicine plus Linggui Zhugan or added formula could improve the total effective rate .Western medicine plus Linggui Zhugan formula could increase left ventricular ejection fraction ,western medicine plus added Linggui Zhugan formula .Meanwhile, only one study indicated that western medicine plus Lingguizhugan or added Formula could reduce the plasma BNP and ANP levels, respectively. Conclusion: By comparison with western medicine, western medicine plus Lingguizhugan or added formula can improve the therapeutic effects in CHF patients.关键词：Linggui Zhugan formula;chronic heart failure;systematic review;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of modfidy Sisi Erhefang on gouty nephropathy. Method: One hundred and four patients with gouty nephropathy were randomized into two groups, 58 patients in the treated group were treated with modfidy Sisi Erhefang,and 46 patients in the control group were treated with allopurinol,symptom,uric acid(UA),creatinine(Cre),urea nitrogen(UN) β2-microglobulin (β2-M) and 24 h urine protein of patiens in two groups were measured before and after treatment for 8 weeks. Result: Improvements of symptoms and decreasing of UA, Cre, UN,β2-MG and 24 h urine protein in the treated group was superior to those in the control group(P<0.05 or P<0.01). Conclusion: Modfidy Sisi Erhefang present good effect in treating gouty nephropathy.关键词：gouty nephropathy;modfidy Sisi Erhefang;traditional Chinese medicine
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Qihuo Therapy combine with artificial liver. On chronic severe hepatitis patients. Method: Thirty-eight patients with chronic severe hepatitis were randomly divided into 2 groups. The eighteen cases in control group were based on conventional medical treatment and supportive liver application on the basis of a single heavy plasma exchange treatment, on the basis of conventional therapy, twenty patients in the treated group were treated with Qihuo therapy every other day after artificial liver therapy, the therapeutic course for both groups was 14 days.Before and after treatment, and the changes of liver function, intestinal endotoxemia and patients’subjective symptoms were observed. Result: The patients in the treated group abdominal distention, fatigue, mouth pain, chills improved significantly, compared with the control group there were significant differences (P <0.05), significantly decreased in both groups of patients and bilirubin endotoxin levels, improve blood clotting function, but the treated group improved more significantly (P <0.05), liver function in both groups was no significant difference. Conclusion: Qihuo therapy is an effective technique which can improve the artificial liver effect on chronic severe hepatitis, and with no adverse reaction.关键词：Qihuo therapy;chronic severe hepatitis;artificial liver therapy;effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Bailing capsule on thyroid auto antibodies in patients with Graves' disease(GD). Method: Sixty first diagnosed cases of GD patients were randomly divided into two groups, control group treated with propylthiouracil and treatment group treated with Bailing capsule combined with propylthiouracil. The levels of FT3,FT4,sTSH,TRAb,TPOAb were measured in two group pre and post treatment. Follow-up 1 year after treatment,observe the recurrence rate of GD. Result: The thyroid function of the two groups was not significant after treatment, in the treatment group thyroid autoantibodies (TRAb, TPOAb) lower than the control group, the difference was significant (P <0.01); follow-up 1 year after withdrawal, the treatment group than the control group recurrence rate was significantly lower, the difference was significant (P<0.05). Conclusion: Bailing capsule can reduce autoantibody levels in patients with GD, and have some regulatory role to the abnormal immune response in patients with GD.关键词：Graves' disease;Bailing capsule;thyroid auto antibodies
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发布时间：2024-01-04 - 摘要：Objective: It has been observed the curative effect to Chronical Functional Constipation (CFC) with the different processed products of Plantain Seed. Method: It had been chosen ninty examples of patients with the chronical functional constipation (CFC), they had been divided into three groups randomly. Then they had been treated with the stir-frying with salt-water of Plantain Seed, the simple stir-frying of Plantain Seed and the raw powder of Plantain Seed differently, and it had been compared and researched the curative effects and the recurrence rates. Result: The comparation of curative effects of these three groups of patients had significant difference ( P<0.05), and the curative effect in the group of the raw powder of Plantain Seed had been the most significant among the total, its effective power (96.67%) and cure rate (90%) had been higher the other two groups, and its recurrence rate of patients’ post-recovery had been the lowest (3.66%). Conclusion: The curative effect to the chronical functional constipation (CFC) with the raw powder of Plantain Seed has been certain and significant, and it has the low recurrence rate. So that the raw powder of Plantain Seed has the value of developing, extending and utilizing extremely.关键词：Plantain Seed;different processed products;chronical functional constipation (CFC);curative effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effect and mechanism of Fuzheng Xiezhuo Baoshen (FZXZBS) decoction on chronic renal failure. Method: According to the time sequence of seeing doctor, a total of 70 patients with chronic renal failure were randomLy divided into treatment group and control group, with 35 patients in each group. Patients in the control group treat with western medicine and patients in the treatment group treat with western medicine coupled with FZXZBS decoction for 3 months to respectively observe the changes in the level of Scr, BUN, Ccr, HA and LN of each group before and after treatment. Result: The total clinical effective rate of treatment group was 77.14% and the rate of control group was 51.43%. There was significant difference between the two groups ( P<0.05). After treatment, the level of Scr and Ccr had significant improved ( P<0.05) and the level of BUN had decreased ( P<0.01) in the treatment group compared with prior treatment. But compared with the control group, the level of Scr, Ccr and BUN had remarkable improved after treatment. The level of HA and LN had obviously decreased ( P<0.05) in the treatment group compared with prior treatment and their level was lower than the control group after treatment ( P<0.01). Conclusion: FZXZBS decoction has a therapeutic effect on CRF and inhibits the progress of renal fibrosis.关键词：chronic renal failure;FZXZBS decoction;renal function;hyaluronic acid (HA);Laminin (LN)
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发布时间：2024-01-04 - 摘要：Objective: For the holistic characteristics of Chinese medicine, the establishment of scientific quality control mode to strengthen control and evaluation the quality of Chinese medicine is explored. Method: The limitations and shortcomings of the China's current quality control mode of Chinese medicine are analyzed to explore how to establish a scientific quality control mode. Result and Conclusion: The ideas and methods are proposed to fully control and evaluate the quality of Chinese medicine by formulating work reference standard of Chinese medicine and using amplitude control methods.关键词：quality standards;working reference standard;quality control mode
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发布时间：2024-01-04 - 摘要：Objective: To analyze the regularity of treating lung cancer with traditional Chinese medicine (TCM) herbs, and supply a foundation for the clinical practice. Method: All the references searched from Chinese biomedical literature database (CBM) were analyzed with one dimension sensitive character frequencies analysis. Then a co-existed herbs network was set up. The final analysis included three of all the networks. Result: Ginseng (Radix Ginseng) and Milkvetch Root (Radix Astragali) were the primary tonic herbs in treating lung cancer. The regularity of prescriptions was consistent to therapeutic principle of supplementing qi and nourishing yin, strengthening the spleen and reducing phlegm, and removing the toxic and stagnation. Conclusion: The regularity was consistent to the etiology and pathogenesis of lung cancer. And it will be useful for the clinical application. Text mining could be used as a reliable tool of TCM research.关键词：lung cancer;traditional Chinese medicine;text mining;regularity of medicine
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发布时间：2024-01-04 - 摘要：To discuss on contribution of the methods of ascending Spleen Yang put forward by LI Dongyuan and the methods of nourishing Stomach Yin created by YE Tian-shi in the forming of thetheory of Spleen and Stomach. Referring the comments on ascending Spleen Yang and nourishing Stomach Yin from LI Dongyuan and YE Tian-shi and other medical literatures. Summarizing the traits and application of the methods of ascending Spleen Yang and nourishing Stomach Yin. Both the methods of ascending Spleen Yang and the methods of nourishing Stomach Yin are equally importantly considered in clinical practice,which canguide clinical practice more effectively.YE Tian-shi created and dwelt on the methods of nourishing Stomach Yin,which made up of deficiencyin themethods ofascending SpleenYang,thus, integrated the methods of ascending Spleen Yang and nourishing Stomach Yin and finally completed and formed the theory of Spleen and Stomach.关键词：Spleen Yang;Stomach Yin;the theory of Spleen and Stomach;forming
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发布时间：2024-01-04 - 摘要：Oridonin, named isodonal and rubescensin A, can be achieved by alcohol extraction, extraction, chromatography and crystallization from the herba of Rabdosia rubescens. Oridonin is able to kill and inhibit different types of cancer cells, such as leukemia, breast cancer, melanoma and so on. Therefore, it’s widely used as anti-humor, antibacterial, anti-inflammatory and antinociceptive agent nowadays. However, due to its poor water-soluble and extremely bitter taste, the clinical application of oridonin is limited. Nanoparticle formulation, a new kind of drug delivery system, could improve its water-soluble and bioavailability effectively. Nanoparticles, liposomes, microemulsion, polymer micelles and nanosuspensions of oridonin have been developed during these years. This paper mainly reviews the latest research and application in nanoparticle formulation of oridonin. It will provide foundation for further research and development of new formulation for oridonin.关键词：Oridonin;nanoparticle drug delivery system;research and application
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the efficacy of ginkgo leaves tablet for angina. Method: Randomized controlled trials (RCT) of the efficacy of ginkgo leaves tablet for angina were collected completely and sieved according to the inclusion criteria. All the dada were analyzed with Rev Man 5.07 software. Result: The curative effect of ginkgo leaves tablet for angina was better than control groups. In clinical symptom P<0.000 01), OR=3.01, 95%CI (2.28, 3.96)],angina symptom P<0.000 01), OR=4.29, 95%CI (3.30, 5.57) and ECG P<0.000 01), OR=2.75, 95%CI (2.33, 3.25)]. Conclusion: Systematic review indicates that ginkgo leaves tablet can improve symptoms of angina, however, further and multi-centre randomized double-blind control trials using ginkgo leaves tablet are still needed because of the limited quality of the included studies.关键词：ginkgo leaves tablet;angina;coronary heart disease;electrocardiogram;clinical effects;systematic review
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