最新刊期
卷 17 ， 期 14 ， 2011
- 摘要：The optimal process of extracting total flavonoids from Fructus Forsythia was studied by single factor test and orthogonal test. The absorption of total flavonoids was determined by uv-spectrophotometry and the standard curve was used to calculate the content. The results showed that the optimum conditions for extracting the total flavonoids assisted by ultrasonic wave are as follow: 60 %ethanol, ultrasonic time 15 min and ultrasonic temperature 60℃.The optimazed method is stable and efficient, the rate of total flavonoids extracted is up to2.58%关键词：ultrasonic wave;fructus forsythia;total flavonoids
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发布时间：2024-01-04 - 摘要：Abstract Objective: To prepare the Vinorelbine liposomes and investigate its release characteristics in vitro．Methods：Vinorelbine liposomes were prepared by thin-film ultrasonication method, the morphology and particle size were investigate. The difference of release characteristics from Vinorelbine liposome to Vinorelbine solution was measured by dialysis method. Results：The particle size of the liposome was149.2nm, the bilayer of the liposome was observed obviously．The release rule of liposome in vitro was subject to the Higuchi model, completely released within 24h, and the release of solution accorded to Higuchi model, completely released within 10h. Conclusion：The VRB liposomes with fine-looking have sustained releasing effect.关键词：vinorelbine;liposome;release in vitro
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of dosage form and administration route to the distribution of ginsenoside Rg1 in serum and brain of the rats. Methods: Compared with ginsenoside Rg1 saline solution administrated from nasal route and oral route, ginsenoside Rg1 nasal spray was administrated from nasal route in rats. The concentration of ginsenoside Rg1 in the serum and brain was detected by HPLC-UV. The data was analysised by the Wagner-Nelson method. Results: The distribution and transport efficiency of ginsenoside Rg1 was improved 5.05 and 2.50 times respectively by nasal administration route. The distribution and transport efficiency of ginsenoside Rg1 was improved 11.80 and 3.35 times respectively by nasal spray. Conclusion: The bio-availability of ginsenoside Rg1 can be improved dramatically by the nasal administration route and nasal spray.关键词：Ginsenoside Rg1;nasal administration;spray
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发布时间：2024-01-04 - 摘要：Objective: Flexible nanoliposomes was prepared with proanthocyanidins to study the relationship between deposition and time of proanthocyanidins in rats'abdominal skin and its topical pharmacokinetics. The function of flexible nanoliposomes in enhancing the transdermal absorption of the encapsulated drug was investigated. Method : Flexible nanoliposomes was prepared with proanthocyanidins using a film dispersion method to determine the relationship between deposition and time of proanthocyanidins in rats' abdominal skin by ultraviolet spectrophotometry, and the pharmacokinetic parameters were fitted by DAS. Result : Depositions of different time in rats' abdominal skin could be fitted with the two compartment model. Conclusion : The active ingredients of proanthocyanidins flexible nanoliposomes can permeate through the skin effectively and lie long time.关键词：proanthocyanidins;flexible nanoliposomes;dermatopharmacokinetic studies of topical skin
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发布时间：2024-01-04 - 摘要：Objective: To investigate the formulation procedure of Acanthopanax senticosus capsule. Method : The influence of different excipients on the hygroscopicity of the main medicines was investigated by screening the best processing technology conditions and inspecting the critical relative humidity, angle of repose,and bulk density. Result : The main medicines and soluble starch, microcrystalline cellulose were mixed by a ratio of 40 ∶45 ∶15, 70% ethanol was added to make materials soft, 20 mesh sieve granulation. The angle of repose of the particles was 33.5°, the bulk density 0.38 g ·mL-1, the critical relative humidity 66%. Conclusion : The molding technology is reasonable and can provide reliable basis for large-scale production.关键词：Acanthopanax senticosus capsule;formation technology;critical relative humidity;angle of repose;bulk density;Parkinson's disease
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发布时间：2024-01-04 - 摘要：Objective: To optimize the preparation process of the aipian, artficial moschus-β-cyclodextrin inclusion compound from the oral solid preparation of Xingnaojing. Method : Aipian and artficial moschus were included by ball grinding method; Box-Behnken response surface method was used to investigate the amount of β-cyclodextrin, inclusion time, the amount of water which effect the preparation process of β-cyclodextrin inclusion compound with the entrapment efficiency of borneol in aipian and muscone in artficial moschus as the index. Design-Expert software was applied to imitate, optimize and verify the process parameter. Result : The optimal process was as follows: the weight rate of borneol-muscon to β-cyclodextrin was 1 ∶8; the weight rate of β-cyclodextrin to water was 1 ∶4; inclusion time was 53 min; the theoretical entrapment efficiency of borneol and muscone was 98.82% and 83.33% respectively. The entrapment efficiency of borneol and muscone was 97.41% and 82.59% respectively by verification. Conclusion : The optimized preparation process of aipian, artficial moschus-β-cyclodextrin inclusion compound by ball grinding method and Box-Behnken response surface method is stable and feasible. The entrapment efficiency is high. It can be used for production.关键词：Xingnaojing;aipian;artficial moschus;β- cyclodextrin inclusion compound;Box-Behnken response surface method
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发布时间：2024-01-04 - 摘要：Objective: To study the preparation method of PNS enteric microcapsules and to investigate the release properties in vitro. Method : The solvent-non-solvent extraction was used to prepare enteric microcapsule of PNS with the polyacrylic acid resin II as the pouch material. The encapsulation efficacy and loading efficiency as well as the properties in vitro release of PNS enteric microcapsules were investigated. Result : Encapsulation efficiency was 90.85%, 90.15%, and 89.06%, loading efficiency was 26.05%, 25.04%, and 25.16% respectively. The release of R1, Rg1 and Rb1 was less than 10% in the artificial gastric juices, and up to 86.08%, 88.76%, and 84.98% in the artificial intestinal juices after 45 min. Conclusion : The microcapsules meet the requirements of pharmacopoeia in vitro release.关键词：Panax notoginseng saponins (PNS);enteric microcapsules;solvent-non-solvent method;release
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发布时间：2024-01-04 - 摘要：Objective: To obtain the optimized inclusion technology of volatile oil from Yukui capsule with β-cyclodextrin (β-CD). Method : The inclusion compound was prepared by saturated peroxide solution method and characterized by differential scanning calorimetry, thermogravimetric analysis and infrared spectrometer. Orthogonal design was applied to optimize preparation conditions. Result : The optimum conditions for inclusion process were as follows: the ratio of volatile oil (mL) to β-CD (g) was 1 ∶10, the inclusion temperature was 50 ℃ and clathration time was 2 h. The inclusion technique was successful, which had been verified by characterizing structure. Conclusion : The developed inclusion process is simple, stable and reliable, with a high inclusion rate and a high yield of inclusion compound.关键词：Angelica Sinensis Radix;Cinnamomi Ramulus;volatile oil;β-cyclodextrin inclusion
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发布时间：2024-01-04 - 摘要：Objective: To optimize the purification condition of active ingredients in Er-xian decotion with macroporous resin. Method : With the total flavonoids, total alkaloids and total saponins in Er-xian decotion as parameters, the D101, DM130, HPD100, HPD400 resins were inspected by dynamic method for the adsorption and desorption performance to screen the best resin and optimize the condition of absorption and desorption for the resin. Result : The D101 resin was suitable to purify the active ingredients in Er-xian decoction. The optimized condition is that: The ratio of medicine to solution is 1 ∶1.5, NaCl concentration is 1.5 mol ·L-1, loading 2.5 BV medicine to D101 resin column, eluting to colourless with 6 BV water, and finally, the active ingredients were eluted by 50% ethanol (5 BV). Conclusion : The active ingredients in Er-xian decotion can be purified simply and practically by D101 resin.关键词：active ingredients in Er-xian decotion;macroporous resin;adsorption and separation
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for content determination of low molecular weight saccharide from Rhizoma Polygonati and optimize the extraction technology. Method : The content of low molecular weight saccharide was determined by phenol-sulfuric acid method. On the basis of single factor test and orthogonal design test, the extraction process of low molecular weight saccharide from Rhizoma Polygonati was optimized using the content of low molecular weight saccharide as index. Result : The optimum extraction technology condition was as follows: 90 ℃, the ratio of solid to solution 1 ∶30, extracted 2 hours every time. Conclusion : This process was simple, reasonable and more productive.关键词：Rhizoma Polygonati;low molecular weight saccharide;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To establish optimal process of extracting total flavonoids from Forsythiae Fructus by single factor test and orthogonal test. Method : The absorption of total flavonoids was determined by uv-spectrophotometer and the standard curve was used to calculate the content. Result : The optimum conditions for extracting the total flavonoids assisted by ultrasonic wave were as follow: 65% ethanol, ultrasonic time 15 min and extraction temperature 60 ℃. The mass fraction of total flavonoids extracted was up to 2.58%. Conclusion : The optimum extraction process was simple, stable, efficient and feasible.关键词：ultrasonic wave extraction;Forsythiae Fructus;total flavonoids;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To select the optimum extraction conditions of Shenju Zhike Pills. Method : The orthogonal design L9 (34) was carried out for the optimum extraction conditions with the yield of the extract and the content of cinnamic acid and hesperidin determined with RP-HPLC as indexes. Result : The best extraction process was as follows: extracted for 2 h, 3 times of extraction and the ratio of sample to water was 1 ∶8; the extraction specific weight was 1.3, the ethanol concentration was 70%. Conclusion : The optimum extraction was rational.关键词：RP-HPLC;cinnamic acid;hesperidin;TLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize the ultrasound-assisted extraction technology of total flavonoids from Trollius chinensis Bunge. Method : The factors such as ethanol concentration, extraction time and liquid-material ratio were optimized by response surface methodology. Result : The highest extraction rate of total flavonoids by ultrasound-assisted extraction was 12.02 mg ·g-1 using ethanol concentration 49 percent, liquid-material ratio of 1 ∶43, extraction temperature at 50 ℃, and extracted for 22 min, which was agreed with model predictions. Conclusion : The extraction process by ultrasound-assisted was stable and reliable.关键词：Trollius chinensis;total flavonoids;ultrasonic-assisted extraction;response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: Choose the best enzyme and craft conditions of hypericin from Hypericum perforatum. Method : The content of hypericin determined by HPLC was used as an index. The orthogonal experiments were used for the optimal craft conditions choosing two of the best enzymes in cellulase, pectinase, xylanase, glucanase, β-glucanase and enzyme (SPE-007A) to investigate the effect on their complex by solid-liquid ratio, enzyme dosage, pH, temperature and the extraction time. Result : The two best enzymes are cellulase and pectinase, the optimum extraction process is as follows pH 4.8, enzyme dosage cellulase-pectinase 2.0%-2.0%, temperature 50 ℃, extraction time 6 h. Conclusion : This method is efficient and stable. It could be used in the future research of Hypericum perforatum.关键词：Hypericum perforatum;hypericin;enzyme extraction;compound enzyme
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发布时间：2024-01-04 - 摘要：Objective: To discuss the effect of dosage form and administration route on the distribution of ginsenoside Rg1 (GRg1) in serum and brain of the rats. Method : Compared with GRg1 saline solution administrated from nasal route and oral route, GRg1 nasal spray was administrated from nasal route in rats. The concentration of GRg1 in the serum and brain was detected by HPLC-UV. The data was analyzed by the Wagner-Nelson method. Result : The distribution and transport efficiency of GRg1 was improved 5.05 and 2.50 times respectively by nasal administration route. The distribution and transport efficiency of GRg1 was improved 11.80 and 3.35 times respectively by nasal spray. Conclusion : The bio-availability of GRg1 can be improved dramatically by the nasal administration route and nasal spray.关键词：Ginsenoside Rg1;nasal administration;spray
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发布时间：2024-01-04 - 摘要：Objective: To optimize the water extraction conditions of Niubang Gancao Tang. Method : The L9 (3)4 orthogonal method was employed to study the best extracted technics through analyzing the content of arctiin by HPLC and the weight of ointment.The determination was carried out on a Hypersil-Keystone C18 column with the mobile phase of methanol and water (43 ∶57), detection wavelength of 280 nm. Result : The best technics of extraction process is 10 times amount of water, decocted 3 times, 2 hours each time. Validatvon test showed that 20.69% arctiin content, dry extract yield was 24.37%. Conclusion : Optimum conditions of water extraction of Niubang Gancao Tang are obtained and the optimized extraction technology is stable and efficient.关键词：Niubang Gancao Tang;orthogonal design;extracted technics;Arctiin;ointment
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发布时间：2024-01-04 - 摘要：Objective: To study the quality standard of Aconiti Lateralis Radix Praeparata slice. Method : According to Chinese Pharmacopoeia, the total ash and acid insoluble ash were determined in Radix Aconiti Lateralis Praeparata slice. The determination of diester and monoester aconitum alkaloids was carried out by RP-HPLC method. HPLC analysis was performed on an Eclipse XDB-C18 (4.6 mm×250 mm,5 μm) column. The mobile phase system was 0.1 mol ·L-1 ammonium acetate (0.5 mL glacial acetic acid per 1 000 mL, phase A) and acetonitrile-tetrahydrofuran (25 ∶15, phase B) with gradient elution. The detection wavelength was at 235 nm. Result : There were obvious differences in content of total ash and acid-insoluble ash. The range of monoester aconitum alkaloids amounts was relatively narrow, but the range of diester aconitum alkaloids amounts was wider. It suggested that the range of diester aconitum alkaloids amounts should be formulated. Conclusion : The method is accurate, reproducible and can be used to enhance the quality control of Aconiti Lateralis Radix Praeparata slice.关键词：Aconiti Lateralis Radix Praeparata slice;total ash;acid-insoluble ash;ester aconitum alkaloids;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for content determination of total saponins in garlic extract. Method : Timosaponin A Ⅲ was used as a reference substance,and vanillin-perchloric acid was applied as coloring reagent to determine the content of total saponins. Result : The calibration curve of timosaponin A Ⅲ showed good linearity in the range of 20.16-181.44 μg,and the regression equation was Y=4.839 6X+0.066 6,r=0.999 5.The average recovery rate was 100.6％,and RSD was 1.5％. Conclusion : The determination method is simple,sensitive and accurate,which can be used for evaluating the quality of total saponins in the garlic extract关键词：garlic extract;total saponins;timosaponin A Ⅲ;colorimetry
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprint of Hugan Tablet to improve its quality standard, and compare the quality of products from different companies using the new established method. Method : The separation was performed on Agilent Zorbax SB-C18 (4.6 mm×250 mm,5 μm) chromatographic column with mobile phase consisted of acetonitrile-water (gradient elution) at a flow rate of 1.0 mL ·min-1. Detecting wavelength was set at 250 nm and the column temperature was set at 30 ℃. Result : Twenty-three characteristic common peaks were confirmed. Relative standard deviations of relative retention time and relative peak area in the method verification were all less than 5%. The similarity of fingerprint of different sources of samples were all more than 0.9. Conclusion : The method is reliable, accurate and can be used as a method to assess the quality of Hugan Tablet.关键词：Hugan Tablet;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the antibacterial activities of different solvent extracts of the fruits of Eucalyptus tereticornis and research identification methods by UV-VIS spectroscopy. Method : Minimal inhibitory concentration (MIC) of the extracts was determined by dilution method. And UV-VIS spectroscopy was used for the determination to provide the basic data for the identification of this plant. Result : The antibacterial activity of the extracts was different to different microbes. Characteristic peaks in the UV-VIS spectrum were identified. Conclusion : Extracts of the fruits of E.tereticornis have good antibacterial activity in vitro. The results of UV-VIS spectrum test provide evidence for the plant identification.关键词：the fruits of Eucalyptus tereticornis;antibacterial;MIC;identification
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发布时间：2024-01-04 - 摘要：Objective: Optimize the extract condition of rutin in Morus alba to establish a quantification method for rutin. Method : Single-factor analysis was applied to optimize the extract condition of rutin in Morus alba HPLC was used in the determination of rutin on C18 column(4.6 mm×250 mm,5 μm) with a mixture of acetonitrile-0.1% phosphoric acid (19 ∶81) as a mobile phase at the flow rate of 1.0 mL ·min-1. Rutin was detected at 360 nm, column temperature was 30 ℃. Result : The optimal extract condition was the solid-to-liquid ratio 1 ∶25, 50% ethanol refluxed for 60 min. The linearity of rutin was in the range of 0.1-2.0 μg. The average recovery rate of rutin was 102.4% with RSD 1.8% (n=6). Conclusion : The determination method was simple, accurate and reliable, it could be used as the determination method of rutin in Morus alba.关键词：RP-HPLC;Morus alba;rutin;determination
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发布时间：2024-01-04 - 摘要：Objective: RP-HPLC fingerprint of Sophora tonkinensis grown in Guangxi Zhuang autonomous region was established. Method : A gradient separated method was applied on C18 column at 25 ℃ with methanol-water as mobile phase. Reference material was oxymatrine. Flow rate was 1.0 mL ·min-1; detection wavelength was set up at 220 nm and changed to 280 nm after 24 min. Result : The results showed that this method had a good repeatability and precision. Conclusion : This result can provide scientific data for analyzing the quality control of Sophora tonkinensis.关键词：Sophora tonkinensis;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constisuents of petroleum spirit part of Mosla chinensis 'Jiangxiangru’. Method :The chemical constituents were analyied and identified by GC-MS. Result : the extra tion was separated by capillary tubing colour spectrum,and 38 peaks affirmed were by WILEY/NBS,NIST02 work station and reference documentation. The major chemical components are thymol(18.31%), 3,13-octadecadien-1-ol (10.26%),carvacrol (8.60%). Conclusion :Analyzing and identifying composition by GC-MS is fast, stable, and has fine reproduction quality, it can be used for composition analysis of Mosla chinensis 'Jiangxiangru’.关键词：Mosla chinensis 'Jiangxiangru’;chemical component;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To determinate the content of shanzhiside methylester in wild and cultivated Lamiophlomis rotate by HPLC. Method :The samples were separated on an WelchromTM Kromasil C18 (4.60 mm×250 mm,5 μm) with acetonitrile(A)- water(B)(0-11 min,9％ A;11-35 min,9％-18％ A;35-45 min,18％ A) colnmu as the mobile phase at a flow rate of 1.0 mL ·min-1. The effluent was monitored at 235 nm and column temperature at 30 ℃ . Result : The linear ranges of shanzhiside methylester was 0.25-2.12 mg ·g-1 (r=0.999 8). The average recoverigs were 100.7％. Conclusion :The contents of shanzhiside methylester in wild and cultivated Lamiophlomis rotate was 1.81, 0.75 mg ·g-1.关键词：HPLC;Lamiophlomis rotata;shanzhiside methylester;wild;cultivated
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of gastrodin in Mateng Zhixuan San by HPLC. Method : High performance liquid chromatography (HPLC) was appatiecl to determinae gastrodin content in Mateng Zhixuan San on RP C18 bonded with silica gel column with acetonitrile-0.1% phosphoric acid water (3 ∶97) as mobile phase,detection wavelength 220 nm, flow rate was 1.0 mL ·min-1. Result : Gastrodin was linear in the range of 0.1-0.8 μg(r=0.999 8), RSD was 0.94%,precision test RSD was 0.76% and reproducibility test RSD was 1.1%. The average recovery was 98.1% and RSD was1.01%(n=6). Conclusion :The method is simple and sensitive for the quality control of this preparation.关键词：Mateng Zhixuan San;gastrodin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The content of aristolochic acid A in the five kinds of medical materials and dispensing granules of Aristolochiaceae family was determined to provide scientific foundation for promoting development of our traditional Chinese medicine particle. Method : HPLC method was applied on YMC-Pack C18 column (4.6 mm×250 mm, 5μm) at 30 ℃ with methanol (A)-0.05% phosphoric acid (B) as the mobile phase; detected wavelength was at 250 nm, and the flow rate was 0.8 mL ·min-1. Result : The content of aristolochic acid A in the five kinds of medical materials, i.e. Akebia stem, Dutchmanspipe Fruit, honeyed Dutchmanspipe Fruit, Radix Aristolochiae and Datchmanspipe Herb was 4.18 mg ·g-1, 0.77 mg ·g-1, 0.46 mg ·g-1, 0.86 mg ·g-1, 0.53 mg ·g-1 respectively. The content of aristolochic acid A in the five kinds of dispensing granules was 1.16 mg ·g-1 for Akebia stem, 0.31 mg ·g-1 for Dutchmanspipe Fruit, 0.10 mg ·g-1 for honeyed Dutchmanspipe Fruit, 0.39 mg ·g-1 for Radix Aristolochiae and 0.03 mg ·g-1 for Datchmanspipe Herb respectively. Conclusion : The method established in this paper has relative adaptability for various kinds of medical materials and dispensing granules containing aristolochic acid A. It can be used for content determination. The production process of traditional Chinese medicine particle prescription has certain effect to composition of medical materials, so it is necessary to establish relevant production monitoring criterion, quality monitoring criterion for ensuring the quality of traditional Chinese medicine particle prescription.关键词：aristolochic acid A;Chinese herbal pieces;traditional Chinese medicine particle
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发布时间：2024-01-04 - 摘要：Objective: To compare and evaluate the quality difference of Pu'er tea from different origins. Method : Three-year-fermentative Pu'er teas from five different origins were selected, and the content of gallicum, caffeine and catechin was determined by HPLC. The content of the total flavonoids was evaluated by NaNO2-A1(NO3)3 method, and their content of total polysaccharides was surveyed by sulphuric acid-phenol method. Result : The gallicum content of the Pu'er tea from five different origins was different, the lowest was 0.308% from Simao, while the highest was 1.482% from Dali Xiaguan. The caffeine content was approximately between 2.366%-3.750%. The catechin content was lower than 0.5%, and the Simao one was the lowest. There were certain differences among the content of total flavones and total polysaccharides of those Pu'er teas. The content of total flavones from Simao was the lowest as 2.93%, while the one from Xishuangbanna was the highest as 5.94%. And the Simao one was the lowest in the content of total polysaccharide as 4.35%, while the Dali Xiaguan one was the highest as 6.18%. Conclusion : There are some differences in chemical composition among the Pu'er tea of different origins.
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发布时间：2024-01-04 -
Determination of Four Components in from Different Habitats and Different Processed Products by HPLC
摘要：Objective: The HPLC method was developed to determine Caffeic acid,Forsythoside A,Forsythin,Arctigenin in Forsythia suspense from different habitats and different processed products. Method : Using RP-HPLC, Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm)columns, mobile phase acetonitrile-0.4% aqueous acetic acid solution by HPLC linear gradient elution method. The detection wavelength 0-20 min, 323 nm, 20-30 min, 330 nm, 30-50 min, 277 nm, 50-55 min, 280 nm, column temperature 30 ℃, velocity 1.0 mL ·min－1, the injection volume 20 μL. Result : The level of four components in F. suspensa from different habitats and different processed products is significantly different. Conclusion : The method can be used for the simultaneous determination of four components in F. suspensa, which provides a scientific basis for quality assessment to F. suspensa.关键词：HPLC;Forsythia suspensa;caffeic acid;forsythoside A;Forsythin;arctigenin;determination;processed products.- 2
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发布时间：2024-01-04 - 摘要：Objective: To establish a new method for determination of sanguinarine and chelerythrine in Macleaya microcarpa (Maxim). Method : Sanguinarine and chelerythrine in Macleaya microcarpa were determined by using high performance capillary electrophoresis (HPCE) with berberine as internal standard. The background electrolyte system selected was 25 mmol ·L-1 disodium hydrogen phosphate and 25 mmol ·L-1 sodium dihydrogen phosphate buffer at pH=7-methanol (2.5 ∶1); applied voltage, 18 kV; temperature, 25 ℃. Diode array detector was set at 200 nm. Result : The linear range of sanguinarine and chelerythrine was 0.012 4-0.082 8 g ·L-1 (r=0.999 8) and 0.018 8-0.125 6 g ·L-1 (r=0.999 6), The average recovery was 98.7% (RSD 1.01%) and 98.4% (RSD 1.27%). Conclusion : The method is simple, rapid and reproducible, and could be used for content determination of sanguinarine and chelerythrine In Macleaya microcarpa .关键词：HPCE;Macleaya microcarpa;sanguinarin;chelerythrine
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of Kangjun Xiaoyan Capsule. Method :The identification of Radix stemonae and Radix et rhizome rhei were carried out by TLC. The content of baicalin was determined by HPLC. Result : The TLC spots were clear and separated well without interference of negative sample. The calibration curve of baicalin was linear in the range of 0.01-0.16 g ·L-1, Y=43 887 599.5X-4 893.8 (r=0.999 9). The average recovery was 98.56% with RSD 1.01% (n=6). Condusion : The method is simple, rapid and accurate. It can be used for the quality control of Kangjun Xiaoyan Capsule.关键词：Kangjun Xiaoyan Capsule;Radix stemonae;Radix et rhizome rhei;baicalin;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To determine active constituents quercetin and rutin in tea of Lycium barbarum leaves by RP-HPLC. Method: Rutin and quercertin were separated on an Kromasil C18 column (4.6 mm×250 mm,5 μm) with methanol-0.04% phosphoric acid (pH was adjusted to 3 with diethylamide) (45 ∶55) as mobile phases. The detecting wavelength was at 360 nm. The flow rate was 1.0 mL ·min-1. Result: The linear ranges of rutin and quercertin were within 0.23-1.84 μg and 0.03-0.24 μg respectively. The recoveries were 98.19% and 97.98% , RSDs were 1.49% and 2.25% respectively. Conclusion: The method is simple, accurate, and reliable, which provides the basis of a better development for the tea of L. barbarum in Xinjiang.关键词：RP-HPLC;tea of Lycium barbarum;rutin;quercetin
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发布时间：2024-01-04 - 摘要：Objective: To analysis of nine kinds of trace elements essential to human in different regions and assess the quality of Impatientis Semen. Method : The content of trcae elements was determined by ICP-MS, the correlation between the content of trace elements in Impatientis Semen was analyzed by SPSS and principal components analysis (PCA) was used to evaluate the quality. Result : The content of useful supplement of Fe, Zn, Mn, Cu was higher. There was significant correlation between different elements. Comprehensive function made by PCA was F=0.209x1+0.256x2+1.194x3+0.236x4+0.267x5+0.044x6+0.196x7-0.084x8+0.263x9. Conclusion : These elements take part in the pharmacodynamic functions of Impatientis Semen and the content of trace elements in different regions was significant different. The results can provide scientific basis for quality evaluation of Impatientis Semen.关键词：Impatientis Semen;trace element;principal components analysis
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发布时间：2024-01-04 - 摘要：Objective: To provide anatomical and chemical evidences for the identification of Chirita eburnean and C. lutea. Method: The paraffin section method and the slide with chloral hydrate were applied for the microscopic identifications of rhizomes and leaves of the two original plants. Result: There were no differences on the microscopic structure of the two medicinal between C. eburnean and C. lutea.However,there are significant differences between chemical characteristics. Conclusion: The article provided the basis for the identification and the further development of the two medicinal plants.关键词：Gesneriaceae;Chirita;Chirita eburnean;Chirita lutea
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发布时间：2024-01-04 - 摘要：Objective: To explore the establishment of non-renal injury model of urinary stone. Method: One hurdred SPF male SD rats were divided randomly into five groups.We administrated the animals chemical pure melamine 0.4-0.7 g ·kg-1 ·d-1continuously 20 d and 30 d,then observed and compared the rate of stone formation and renal damage. Result: Administration of chemical pure melamine 0.4,0.5 g ·kg-1 ·d-1,20,30 d,gained the highest rate of bladder stones formation,respectively,60% and 50% in 20 d,50% and 60% in 30 d.The difference of rate of bladder stones formation between group 0.4 g ·kg-1 ·d-1 and 0.5 g ·kg-1 ·d-1,and between group 0.4,0.5 and 0.6,0.7 g ·kg-1 ·d-1,blank control group were significant(P<0.05).Rate of bladder stone formation was not correrated with the increase in dose and time.The group 0.4 g ·kg-1 ·d-1is the only group that has no significant difference in the compare of renal and bladder index,creatinine,urea nitrogen,uric acid with the control group. Conclusion: Administration of chemical pure melamine 0.4 g ·kg-1 ·d-1 did not cause kidneys injury and was able to induce high rate of SD rat bladder stone formation(60%).So this method is suitable for the establishment a non-renal injury model of urinary stones.关键词：urinary stone model;melamine;bladder stone;rats
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Inula helianthus-aquatica.Eleven compounds were isolated from the title plant. Method : These compounds were isolated by various chromatography methods such as silica gel, RP-18 silica gel, Sephadex LH-20 gel, HPLC, et al. Their structures were elucidated by spectral analysis like MS, NMR. Result : Seven sesquiterpene lactones were isolated and elucidated as 11α, 13-dihydrotomentosin (1), sundiversifolide (2), 8-epi-xanthatin-1β,5β-epoxide (3), ivaxillin (4), 11, 13-dehydroivaxillin (5), 1β-hydroxyalantolactone (6), carabrone (7). Four triterpenes were isolated and elucidated as taraxasterol 3-O-acetate (8), maniladiol 3-O-palmitate (9), 16β-hydroxylupeol 3-O-tetradecanoate (10), arnidiol (11). Conclusion : All the compounds were isolated from I. helianthus-aquatica for the first time.关键词：Inula helianthus-aquatica;chemical constituents;sesquiterpene lactones;triterpenes
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发布时间：2024-01-04 - 摘要：Objective: To make clear the peak content of total flavonoids and total polysaccharide in the leaf and fruit of Eucommia ulmoides, so as to provide evidence for the optimal harvest of the fruit of E.ulmoides. Method: Total flavonoids extracted with 70% ethanol by reflux from leaves and fruits of E.ulmoides in different harvest periods were determined by UV spectroscopy at 506 nm. Total polysaccharide from leaves and fruits of E. ulmoides in different harvest periods were determined by phenol-sulfuric acid method and measured by ultraviolet spectrophotometer at 481 nm with glucose as a reference substance. Result: The flavonoids content in leaves and fruits of E. ulmoides during different harvest periods were significant different by UV spectroscopy. The highest flavonoids content of leaves and fruits during different harvest periods of E. ulmoides was found in April, and then declined until July and a little rise again. The content of flavonoids stabilized after September. There were significant differences about total polysaccharide in leaves and fruit of E. ulmoides in different seasons. The total polysaccharide content of the leaves and fruits of Eucommia in different harvests were highest in March. The content of polysaccharide of the Eucommia leaf gradually decreased after May. And then, total polysaccharide content of Eucommia fruits would stabilize from August to November. Conclusion: It can be obtained from this experiment that the best harvest period of the leaves and fruits of Eucommia is in April.关键词：Eucommia ulmoides leaf;Eucommia ulmoides fruit;total flavonoids;total polysaccharide;different harvest periods
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发布时间：2024-01-04 - 摘要：Objective: To investigate the volatile constituents in Hypericum kouytchense. Method : The volatile constituents from leaves of H. kouytchense were analyzed by SPME(Solid-phase Microextraction), coupled with GC-MS. A quantitative analysis in percent was performed by peak area normalization measurements. Result : Thirty-five compounds (94.46%) were identified from the volatile constituents in H. kouytchense. Conclusion: The main volatile constituents in H. kouytchense were β-selinene (12.30%), α-selinene (11.75%) and caryophyllene (10.71%).关键词：Hypericum kouytchense;volatile constituents;GC-MS;solid -phase microextraction
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the alcohol extract of root bark of Celastrus angulatus. Method: The compounds,whose structures were confirmed by NMR spectra,were separated and purified by silica gel and preparative HPLC. Result: The structures of eleven compounds were conformed,they were celahin B(1),celastrine A(2),celahin D(3),angulatin E(4),angulatin A(5),celangulatin C(6),celangulin XIX(7),angulatin P(8),angulatin H(9),celangulin III(10),and angulatin J(11). Conclusion: Compound 1-3 were first found in C.angulatus.关键词：Celastrus angulatus;alcohol extract;chemical constituents;isolation and identification
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of the L-dopa methyl ester (L-DME) on the pattern visual evoked potentials (PVEP) and expression of NGF in neurons of the visual cortex of kittens with strabismic amblyopia, and explore the therapeutic effect of L-dopa methyl ester on amblyopia and its action mechanism. Method : Thirty normal kittens were randomly divided into 6 groups: normal control, model control group,positive control (L-dopa, 40 mg ·kg-1),low dose of L-dopa methyl ester (20 mg ·kg-1), medium dose of L-dopa methyl ester (40 mg ·kg-1), and high dose of L-dopa methyl ester (80 mg ·kg-1). The surgery for producing iatrogenic convergent strabismus was performed on 4 week old kittens (normal control group excluded). After the confirmation of the development of amblyopia by pattern visual evoked potential, L-dopa methyl ester, L-dopa and normal saline were given to the corresponding animals respectively. The PVEP of amblyopia eyes was observed after one month, and the technique of immunohistochemical determination was used to detect the expression of nerve growth factor (NGF). Result : Compared with the normal control group, the P100 wave latency delay and the amplitude of PVEP were decreased in the model control group and the number of positive cells in the model control group was significantly reduced (P<0.05). Compared with the model control group, P100 wave latency was shortened and amplitude increased and immune-positive cells in the treatment group increased (P<0.05). Conclusion : L-dopa methyl ester can significantly improve conduction and sensory function in model of strabismic amblyopia kittens. The mechanism may be related to the increased amount of L-dopa methyl ester into the cerebral cortex and regulation of the expression of NGF.关键词：L-dopa methyl ester;strabismic amblyopia;nerve growth factor;visual cortex;kitten
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发布时间：2024-01-04 - 摘要：Objective: To observe the patterns of changes in syndrome of Chinese medicine for rat model of type 2 diabetes with insulin resistance. Method : The rat model was established by feeding with high calories and fat diet together with peritoneal injection of STZ. SD rats were randomly divided into the control group (50 rats) and the model group (60 rats). The changes in appearance, behavior and weight of rats during the process of experiment were observed. At the end of the 0, 6, 8, 10 and 12 week, ten rats each time were killed and blood samples were collected respectively to detect the (FPG) and Fins, and to calculate the insulin resistance index (IRI) and insulin sensitivity index (ISI). Result : 1) Appearance and behavior: Compared with the control group, the weight of the model group gained up obviously at 4th week, and lowers continually from the 9th week, higher obviously than that of the control group. During the 6 w to 8 w, the model rats appeared in lethargy with narrowed eyes, accidie, loss of hair, increased drinking volume, dejecta desiccation, sweatiness and wateriness at tail. During the 9 w~10 w, the model rats appeared fatness, extrados, gather together, narrowed eyes, accidie, slow action, fatness in hair which based on syndromes at first phase; during the 11-12 w, the model rats appeared continually loss of hair and turn into yellow color at stern, dark-purple tongue, and ome petechias appeared at the tail from top to root. Glucose metabolism: Compared with the control group, the FPG, Fins, IRI levels of the model group were increased continually from the end of 6th week, reached its top at the end of 12th week. The IAI level was decreased obviously from the end of 6th week, reached its bottom at the end of 12th week. Conclusion : The rat model of type 2 diabetes with insulin resistance established by feeding high calories and fat diet together with peritoneal injection of STZ may appear the characters of dynamic evolutionary syndromes in Chinese medicine during different phases. The dynamic progression of syndromes in Chinese medicine is from the simultaneous deficiency of both qi and yin to simultaneous deficiency of both qi and yin with concurrent turbid phlegm, and the lastly to the simultaneous deficiency of both qi and yin with concurrent turbid phlegm and blood stasis.关键词：type 2 diabetes;insulin resistance;rat model;syndrome in Chinese medicine
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发布时间：2024-01-04 - 摘要：Objective: To research the effects of Jingqianshu granule (JQSG) on the expression of serotonin transporter(5-HTT)of premenstrual syndrome (PMS) model rats with liver-qi stagnation in different brain regions and the mechanism at molecular level. Method : SPF female SD rats were randomly divided into normal control group, model group, JQSG group(10 g ·kg-1)and fluoxetine group(0.002 5 g ·kg-1).The PMS model rats with liver-qi stagnation were induced by bondaging the limbs. Drugs were given for 5 days, and the effects were evaluated by open-field test and sucrose preference test. We analyzed the expression of 5-HTT in hippocampus and hypothalamus by the method of RT-PCR and Western blot. Result : Compared with the model group, the score of open-field and saccharine preference index of JQSG and fluoxetine group were markedly increased (P<0.05, P<0.01). The treatment could improve the expression of 5-HTT in hippocampus and hypothalamus (P<0.05,P<0.01)and the two treated groups showed no significant difference. Conclusion : JQSG can regulate the 5-HTT expression level in hippocampus and hypothalamus in model rats, which may be one of the mechanisms responsible for treating PMS with liver-qi stagnation.关键词：premenstrual syndrome with liver-qi stagnation;5-serotonin transporter;Jiangqianshu granule
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Xiefei Pingchuanling and its decomposed formulas on serum changes of endotoxin (ET) and tumor necrosis factor-α (TNF-α) in model rats with 'interior and exterior relationship between the lung and the large intestine’. Method :Seventy SD rats were randomly divided into 7 groups: the normal group, the model group, the unhitch group, the Sanao group, the Santong group, the Sanhuo group and the Xiefei Pingchuanling group. The rat model of 'interior and exterior relationship between the Lung and the Large intestine’ was established to intestinal obstruction by incomplete ligation of the rectum in vitro. The rats in model group were killed after 3 days. The Sanao group was treated by Sanao decoction with the crude drug dosage of 3.92 g ·kg-1 intragastrically, the Santong group was treated by Santong decoction with the crude drug dosage 7.11 g ·kg-1 intragastrically, the Sanhuo group was teated by Sanhuo decoction with the crude drug dosage 10.54 g ·kg-1 intragastrically, the Xiefei Pingchuanling group was treated by Xiefei Pingchuanling decoction with the crude drug dosage 16.66 g ·kg-1 intragastrically. The rats were killed after 3 days of ig. The changes in serum ET and TNF-α were observed to investigate the interventional effect of Xiefei Pingchuanling and Its decomposed formulas. Result : The serum levels of ET and TNF-α were increased significantly in the model group(P＜0.01). The serum levels of ET and TNF-α were decreased in the other groups compared with the models(P＜0.01). The synergies existed in some groups. Conclusion :Xiefei Pingchuanling and its decomposed formulas can protect the lung tissue, alleviate pathological change and prevent the tissue of the model rats from endotoxemia.关键词：Xiefei Pingchuanling;interior and exterior relationship between the lung and the large Intestine;endotoxin;tumor necrosis factor-α;rat
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Tangzhiping on the glucose-toxicity, lipotoxicity and the oxidative stress of pancreatic tissue in type 2 diabetic rats. Method : Type 2 diabetes mellitus was induced in male SD rats by feeding with high fat diet combined with intraperitoneal injection of low-dose streptozotocin (30 mg ·kg-1). Forty diabetic rats were randomly assigned to the model, Tangzhiping, alpha lipoic acid (ALA) and normal control groups. Rats in Tangzhiping group were performed intragastric administration of 20 g ·kg-1 ·d-1 crude drug of Tangzhiping, and rats in ALA group were administrated with 20 mg ·kg-1 ·d-1 ALA, other two groups were administrated with distilled water by equal volume. All four groups had been treated for eight weeks. Body mass (BM), fast plasma glucose (FPG), glycolated hemoglobin (HbA1c), serum free fatty acid (FFA) and fast insulin (FINS) of rats were measured after eight weeks. And meanwhile superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malonaldehyde (MDA) of pancreatic tissue were measured. Pathologic changes of pancreatic tissue were observed. Result : Compared with the model group, the levels of BM, FPG, HbA1c, FFA, FINS, SOD, GSH-Px and MDA of rats in both Tangzhiping group and ALA group were all significantly improved (P<0.05 or 0.01) and pathological observation showed that the number of pancreas islet increased significantly and the injured pancreas were partially repaired in both groups. The effects of Tangzhiping group on BM, FPG, HbA1c, FFA, FINS were superior to those of ALA group, the differences were all significant (P<0.01). Conclusion : Tangzhiping can protect effectively the pancreatic islet β cells to improve insulin secretion and glucolipid metabolism by effectively inhibiting the glucose-toxicity, lipotoxicity and the oxidative stress of pancreatic tissue in type 2 diabetic rats.关键词：type 2 diabetic rats;pancreatic islet β cells;Tangzhiping;free fatty acid;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Shugan Liangxue decoction (SGLXD) on MCF-7 and T47D cells proliferation, and evaluate the effects of SGLXD on the enzymatic activity and mRNA expression of steroid sulfatase (STS). Method: Estrogen receptor positive breast cancer MCF-7 and T47D cells were cultured and treated with different concentrations of SGLXD for 24 h. Cell proliferation was evaluated by MTT assay, and the mRNA expression of STS was investigated by RT-PCR. After transient co-transfection with plasmid pERE-TK-Luc and pRL-TK in MCF-7 and T47D cells, and treated with different concentrations of SGLXD for 24 h, we evaluated the enzymatic activity of STS by dual-luciferase reporter assay (DLR). Result : By MTT assay, SGLXD inhibited the cell proliferation of MCF-7 and T47D dose-dependently, the IC50 values were 46.08 g ·L-1 and 22.98 g ·L-1. SGLXD down-regulated the expression of STS mRNA at higher concentrations significantly. In MCF-7 and T47D cells, the enzymatic activity of STS was significantly inhibited by SGLXD dose-dependently. Conclusion : SGLXD can inhibit the cell proliferation of MCF-7 and T47D, this effect may be associated with the inhibitory effects of SGLXD on the enzymatic activity of STS and the expression of STS mRNA.关键词：Shugan Liangxue decoction;steroid sulfatase;breast cancer
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发布时间：2024-01-04 - 摘要：Objective: To observe the impact of patients in disease of idiopathic thrombocytopenic purpura (ITP) of platelet count, marrow megakaryocyte count and class, T lymphocyte subset in peripheral blood on treatment with Yiqi Tongyang decoction. Method : It is designed to use guinea pig anti-mouse serum (APS) to inject to the mouse in order to induce the ITP mouse model. Then Yiqi Tongyang decoctions treatment group with 3 levels of greate, media and small is given.Separately to the treatement, the normal control group and model control group are also set, and all 3 group are maintained its treatment for continuel 14 days. The blood cell counter is used to detect platelet count, to observe megakaryocyte configuration by microscope, to count the megakaryocyte in mouse thighbone by plastic embedding and to detect T lymphocyte subset by cytometer. Result : In model mouse group, platelet, CD3+CD4+T cell are reduced significantely, and CD3+CD8+T cells are significantly increased. In Yiqi Tongyang mouse groups, platelet count increased significant, producing platelet megakaryocyte and CD4+/CD8+ were obviously increased. Megakaryocyte count reduced, CD3+CD4+T cell increased, and CD3+CD8+ T lymphocyte are significantly decreased, and CD4+/CD8+value is increased. Conclusion : Yiqi Tongyang decoction improves platelet count in ITP model mouse group, increase platelet producing megakaryocyte ratio, meliorate unbalance of T lymphocyte, that means the Yiqi Tongyang decoction is approved to be effective of treating ITP patients clearly in this study.关键词：Yiqi Tongyang decoction;idiopathic thrombocytopenic purpura;animal model
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发布时间：2024-01-04 - 摘要：Objective: To investigate the cytotoxic effect of joint treatment of three Cirsii Japonici Herba components on human gastric carcinoma MKN45 cells. Method :MTT assay of rat lymphocyte and MKN45 cells proliferation in vitro were used to determine the effective components of Cirsii Japonici Herba. Orthogonal test were used to determine the optimum proportion of three components of Cirsii Japonici Herba on human gastric carcinoma MKN45 cells. Result : The combined treatment of Cirsii Japonici Herba polysaccharides and saponins (2#+5#) significantly improved the lymphocyte proliferation of the cells. The cytotoxic activity of the combined treatment of Cirsii Japonici Herba oil, polysaccharides and saponins (1 ∶1 ∶1) to MKN45 cells (IC50 13.3 μg ·mL-1) was obviously stronger than that of the combination of any two components or Cirsii Japonici Herba oil used alone (IC50 35 μg ·mL-1). The order of cytotoxic activity of three components of Cirsii Japonici Herba is oil>polysaccharides> saponins. The optimum proportion of three Cirsii Japonici Herba components on MKN45 cells was oil ∶polysaccharides ∶saponins 1 ∶2 ∶1. Conclusion : The combined treatment of three Cirsii Japonici Herba components had the strongest killing activity to human gastric carcinoma MKN45 cells. Except the effects of Fuzheng and Quxie come from the combined treatment, cytotoxicity and irritation of the Cirsii Japonici Herba oil were also decreased by the combined treatment.关键词：Cirsii Japonici Herba oil;Cirsii Japonici Herba polysaccharides;Cirsii Japonici Herba saponins;human gastric carcinoma MKN45 cells;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To investigate morphologic effect of Tangshenqing on kidney of rats with diabetic nephropathy. Method :SD rats were induced diabetic nephropathy by STZ then randomly divided into normal control group, the model group, the positive group, the high dose of Tangshenqing group, the medium dose of Tangshenqing group and the low dose of the group. They were 187, 93.5 and 46.75 mg ·kg-1 respectively. After twelve weeks' oral drug administration, the rats were killed and the kidneys were harvested for hematoxylin and eosin and the periodic acid-sliver dyeing. The renal lesions were classified and scored under light microscope. The relative area of mesangium matrix was measured under the periodic acid-sliver dyeing. Result :The renal lesions of the high dose of Tangshenqing group was lightened than the model group. Mesangium matrix relative area of the high dose of Tangshenqing was obviously reduced than the model group (P<0.05). Conclusion :Tangshenqing may relive the extent of mesangium matrix hyperplasia for rats with diabetic nephropathy.关键词：Tangshenqing;diabetic nephropathy;morphology
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发布时间：2024-01-04 - 摘要：Objective: To analyze and compare composition and content of volatile oils extracted from seven samples of Ligusticum sinensis, Conioselinum tataricum and Ligusticum jeholense and observe the vascular activity of them in vitro. Method : Seven samples were collected from different producing areas.Hydrodistillation was used for the preparation of volatile oils, and was performed according to the method described in the China Pharmacopoeia (2010).The volatile oils of the seven samples were bioassayed by using KCl-induced aorta contraction assay in vitro as well as chemically analyzed by using GC-MS analysis methods, the components were quantitatively determined with normalization method. Result : eighty-two constituents from volatile oils in total were selected by GC-MS, which occupied more than 94% of included samples in average, including fourteen Common components. Moreover, the research of in vitro vaso-relaxant effect indicated that the volatile oils of all samples possessed vaso-relaxing properties with different effectiveness. and the dose-response curves were all nonlinear, its median effective dose (ED50) were in the range of 30.4-400.1 μg ·mL-1. Conclusion : Chemical constituents and the amounts of these compounds of volatile oils are varied obviously according to different samples, The vasorelaxing effectiveness is also obviously dissimilar.关键词：Ligusticum sinensis;Conioselinum tataricum;volatile oil;GC-MS;thoracicaorta;vascular activity
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发布时间：2024-01-04 - 摘要：Objective: To observe effect of Qiwei Qingyan aerosol for treatment and prevention and H1N1 virus as well as the efficacy when combining with tamiflu. Method: The method of cell and tissue culture was utilized to carry out an observation on the cytopathy effect(CPE) led by influenza virus FM1 with the aerosol of different concentrations in vitro. The swing influenza A(H1N1)virus FM1 was adopted to establish pneumonia model in mice by nasal dropping method to detect the efficacy of the aerosol for treatment in vivo. In addition, Combining the aerosol with tamiflu (both in 50% dose) to inspect the efficacy for efficiency improvement and toxicity depression of Chinese traditional medicine. Result : ①The maximal nontoxic concentration (TC0) of Qiwei Qingyan aerosol was 0.192 g ·L-1 to Hela and Hep-2 cell. Within this concentration range it could relieve CPE induced by influenza virus, which was comparatively weak.②After 7-days successive high-dose medication of Qiwei Qingyan aerosol, the TNF-α level in serum of mice infected by H1N1 virus could be decreased apparently. Meanwhile, the IL-2 level could be increased significantly.③A combination of half dose tamiflu and moderate dose aerosol lowered the lung index of infected mice. As well, a lesser extent of lung tissue lesion was discovered 4.The ability of the combination to decrease the neuraminidase(NA) activity in the blood of the infected animals was superior to tamiflu solely used. Conclusion : By affecting the function of mice's immune system, Qiwei Qingyan aerosol improved the pneumonia symptoms induced by influenza virus and decreased the lung index of H1N1 infected mice. Furthermore, the combination with tamiflu possessed the effect of improving the efficiency and depressing the toxicity.关键词：influenga A H1N1;FM1;Qiwei Qingyan aerosol;combined use of drugs;NA;TNF-α;IL-2
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of isorhamnetin on cell cycle and cell apoptosis in H2O2-induced injury of human umbilical vein endothelial cells (HUVECs). Method :Cultured CRL-1730 cell was commonly incubated at 37 ℃ in 5%CO2 for 24 hours with isorhamnetin of different concentrations, then CRL-1730 was injured by H2O2 for 4 hours. Cell viability was measured by MTT assay. The rate of apoptosis and cell circles were detected by flow cytometry (FCM). Result : Iisorhamnetin could increase the cell viability in a concentration-dependent manner. Compared with the model group, the high dosage group showed A of 0.503 (increased 0.044),with significant difference (P<0.01). Low dosage group was no significant difference, while the middle dosage group showed A of 0.48 (increased 0.027), with significance (P<0.05). Isorhamnetin could decrease the cell apoptosis in a concentration-dependent manner,especially decrease the early apoptosis. Compared with the model group apoptosis rate of 77.78%, the apoptosis in high dosage group was 69.28% and the middle dosage group was 72.50%. Both had statistical significance (P<0.05). Isorhamnetin could inhibit the decrease in CRL-1730 caused by H2O2, showing by the cell cycle ratio of G0/G1. In the model group the ratio was 82.23%, in the high, middle and low dosage groups of isorhamnetin they were 69.43%, 67.05% and 69.56% accordingly. All of them had significance. Isorhamnetin increased the percentage of cells in S phase and C2/M phase, the percentage of cells of S phase and C2/M phase of the model group was 11.77% and 1.91% respectively, while the high dosage was 23.39% and 7.18%, the middle dosage was 29.73% and 3.23%, and the low dosage was 27.42% and 3.01%. Conclusion : Isorhamnetin has protective effect on HUVECs injury induced by H2O2. The mechanism may be related to its antioxidant activity.关键词：isorhamnetin;H2O2;CRL-1730;cell circle;rate of apoptosis
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发布时间：2024-01-04 - 摘要：Objective: Yupingfeng San (YPFS) is a traditional formula used for allergy such as asthma, allergic rhinitis, and urticaria. Mice Th2 cell-mediated allergic contact dermatitis was utilized in this study to explore the effect of YPFS on allergic inflammatory. Method : The mice were topically sensitized on the shaved abdomens with 1.5%FITC solution on abdominal skin in the day 1 and day 2 and elicited on the right ear with 0.5%FITC solution at day 6. Mice were treated with either YPFS or normal saline from day 1 to day 5 (induction phase). Ear thickness was measured using a calibrated thickness gauge 24 h after the elicitation. Ear pathohistological examination was carried out by HE staining and IL-4 level of ear tissue was detected by ELISA. Result : The ear thickness, measured either by calibrated thickness gauge or under the microscope, was inhibited significantly by administration with YPFS 6.5 g ·kg-1 and YPFS 13 g ·kg-1 in induction phase. The pathohistological examination showed that the ear inflammation and infiltration of inflammatory cells was significantly reduced by YPFS. The levels of IL-4 in ear tissue were significantly lower in YPFS 6.5 g ·kg-1 group compared with that in model group. Conclusion : Administration with YPFS in induction phase could inhibit Th2 cell-mediated allergic contact dermatitis in mice significantly, and the mechanism may be related with regulating IL-4.关键词：Yupingfeng San;fluorescein isothyocyanate;allergic contact dermatitis;ear swelling;interleukin-4
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发布时间：2024-01-04 - 摘要：Objective: To observe anti-cold diarrhea effect of Aconiti Lateralis Radix Praeparata on mice with spleen Yang deficiency syndrome. Method: Mice were fed with decoction of Sennae Folium for 29 days to induce the model of spleen Yang deficiency syndrome. After modeling,Aconiti Lateralis Radix Praeparata suspension was given(in the doses of 0.42,0.84, 1.68 g ·kg-1) for 10 days. After treatment, small intestinal propulsion rate (by charcoal powder propelling experiment), survival time under -10 ℃ (by cold tolerance experiment) and anoxia condition (anoxia tolerance experiment) were recorded, respectively. The effects of three dosages of Aconiti Lateralis Rradix Praeparata suspension on small intestinal propulsion, cold tolerance and anoxia tolerance were investigated. Result: After administration of Aconiti Lateralis Radix Praeparata(0.84,1.68 g ·kg-1), an inhibition of small intestinal propulsion ( P <0.05) and body weight-increasing ( P <0.01) in mice with spleen Yang deficiency were observed when compared with the model group. The survival time under -10 ℃ ( P <0.05) and anoxia condition( P <0.05) were pronouncedly prolonged by the administrating with Aconiti Lateralis Rradix Praeparata at the dose of 1.68 g ·kg-1. Conclusion: Aconiti Lateralis Radix Praeparata has an obvious therapeutic efficacy on mice with spleen Yang deficiency syndrome.关键词：Aconiti Lateralis Rradix Praeparata;spleen Yang deficiency syndrome;small intestinal propulsion;cold tolerance;anoxia tolerance
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shugan Jianpi Recipe on 5-hydroxytryptamine(5-HT),calcitonin gene related peptide(CGRP) in rats with diarrhea predominant irritable bowel syndrome(D-IBS). Method: Sixty Wistar rats were randomized into normal group,model group,low,middle and high-dose groups of Shugan Jianpi Recipe,and dicetel group.Cold-binding applications were given to establish similar model of D-IBS.Violet spectrophotometer was used for determining the levels of 5-HT in serum.RIA was used for measuring the levels of CGRP in serum.The expressions of 5-HT and CGRP in intestinal and colon mucosa and colon tissue were detected by immunohistochemical SABC method. Result: In the model group,the 5-HT level increased,CGRP decreased,compared with those in the normal group(P<0.05).The Shugan Jianpi Recipe's middle,high-dose increased 5-HT and decreased CGRP significantly,compared with that in the model group(P<0.01). Conclusion: The therapeutic mechanism of Shugan Jianpi Recipe for D-IBS is probably related with adjustment of 5-HT and CGRP in serum and colon tissue.关键词：Shugan Jianpi Recipe;diarrhea predominant irritable bowel syndrome;5-hydroxytryptamine;calcitonin gene related peptide;colonic mucosa;rats
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发布时间：2024-01-04 - 摘要：Objective: Kaiyu capsule is a new compound preparation which consists of 8 traditional Chinese herbs. The purpose of the study was to investigate the effects of Kaiyu capsule on depression-like animal models. Method : ICR mice were randomly allocated to five groups: control, Kaiyu capsule low, middle and high dose groups (6.6,13.2, 26.4 g ·kg-1 accordingly), and Paroxetine (positive control). After intragastric administration for 14 d, the antidepressant effect of Kaiyu capsule was evaluated by using mice tail suspension test, mice forced swimming test, 5-hydroxytryptophan (5-HTP)-induced head-twitches and reserpine reversal model in mice. Result : Compared with control group, the middle and high doses of Kaiyu capsule significantly decreased the accumulative immobility time in mice tail suspension test(P<0.05). High dose Kaiyu capsule obviously increased the head-twitching number induced by 5-HTP(P<0.01), and shortened the latency period(P<0.05). After sc reserpine, the mice appeared physical signs such as blepharoptosis, akinesia and hypothermia. The middle and high doses of Kaiyu capsule significantly ameliorated the blepharoptosis and akinesia(P<0.01), and decreased the range of hypothermia in reserpine-induced model mice(P<0.01). Conclusion : Kaiyu capsule has the significant antidepressant effect, and the mechanisms may be related to the enhancement of serotonergic and noradrenergic functions.关键词：depression;tail suspension;forced swimming;reserpine;5-hydroxytryptamine;noradrenaline;Kaiyu capsule
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发布时间：2024-01-04 - 摘要：Objective: To study on the influence and mechanisms of N-Maleoyl-L-Valine Curcumin Ester (MVC) on MGC-803 cell cycle. Method :The role of MVC on cell growth was tested in vitro by MTT. Cell cycle was analyzed by flow cytometry. The level of the protein involved in cell cycle arrest metabolism was detected by western blotting. Result :MCV markedly inhibidited MGC-803 cell growth and significantly blocked cell cycle progression in the S and G2/M phase in MGC-803 cells. The expression of p21WAF1/CIP1 was increased, and while expression of Cdc 2 was decreased in MGC-803 cell treated with 20 μmol ·L-1 MCV for 24 hour. Conclusion : MCV induces S and G2/M arrest in MGC-803 cells via a mechanism linked with up- regulation of p21WAF1/CIP1and dowh-regulation of Cdc 2.关键词：N-maleoyl-L-valine curcumin ester;cell cycle;MGC-803 cells
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发布时间：2024-01-04 - 摘要：Objective: To observe the influences of Compound Prescription Capsule of Silkworm Powder(CCSP) on glycometablism and lipid metabolism in type 2 diabetic rat. Method : Male Sprague-Dawley rat model of type 2 diabetes was induced by high-fat diet and low dose streptozotocin (STZ). Seventy-two model rats were separated according to plasma glucose levels into models, normal control group, model group, low dose group, middle dose group, high dose group and Acarbose tablets group. After 6 weeks, all animals were tested respectively the levels of fasting plasma glucose (FPG) and fasting insulin (FINS), cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C), malonaldehyde (MDA), superoxide dismutase (SOD). Result : Compared with model group. Low, middle and high doses of CCSP could remarkably lower the levels of FPG, FINS, TG, TC, LDL-C and MDA, obviously improve the Home-IR, SOD and HDL-C(P<0.05 or P<0.01). It had the amendment of high-insulin. Conclusion : CCSP can effectively adjust the glyco-lipid metabolim, thus to abate insulin resistance.关键词：type 2 diabetes mellitus;Compound Prescription Capsule of Silkworm Powder;fasting plasma glucose;plasma insulin;plasma lipocy;rat
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发布时间：2024-01-04 - 摘要：Objective: To investigate formulating principle based on Miao nationality medicine theory of Tongbi Paizhuo proved recipe(TBPZ), study the medicine efficacy to treat acute gouty arthritis. Method : Wistar rats were divided into groups (n=10 each) at random, the group is respectively normal, positive control medicine, TBPZ high dose (6 g ·kg-1), TBPZ low dose (3 g ·kg-1) and model. Rat model of acute gouty arthritis was established by MSU intraarticular injection. Medicines were ig given separately for 5 days or 11 days according to experimental requirements. Toe swelling rate and serumuric acid level in rats were determined. Analgesic effect of the recipe was evaluated by mice stimulated with acetic acid or hot plate. Result : TBPZ high dose group and positive control medicine group could reduced toe swelling rate markedly (P<0.01), the low dose could reduced toe swelling rate obviously (P<0.05). TBPZ high and low dose both could reduce the serumuric acid level markedly in acute gouty arthritis rat (P<0.01), which proved recipe had analgesic effect significantly in mice stimulated by acetic acid or hot plate. Conclusion : TBPZ could significantly reduce the serumuric acid level and toe swelling rate in acute gouty arthritis rat, and has certain analgesic effect in mouse. Formulating principle, such as 'using vine to activating blood circulation’ and 'excrete zhuo and luan’ of Miao nationality medicine for treating thermal- rheumatic, can be used as a reference to treat gouty arthritis.关键词：Tongbi Paizhuo proved recipe;gouty arthritis;serumuric acid;analgesic effect
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of arsenic trioxide (As2O3 ) with vitamin C(Vit C) on the subcutaneously transplanted tumor of drug resistant human lung tumors in nude mice. Method : The subcutaneously transplanted tumor models of drug resistant human lung tumors in nude mice were established and then were divided at random into 5 groups: NS,Vit C(250 mg ·kg-1), As2O3 (1.5, 3.0 mg ·kg-1), and combining As2O3 with Vit C. They were injected intraperitoneally for two weeks. The weight of mice and volume of tumor pre- and post-administration were measured. All mice were killed after administration, and measured the mass of tumor and calculated the tumor inhibition. Tumor tissue was examined by pathological method. RT-PCR and Western blot were used to detect the vascular endothelial growth factor and survivin. Result : The tumor inhibitory rate of Vit C, As2O3 (1.5, 3.0 mg ·kg-1), and combining As2O3 with Vit C were 3.42％, 42.3％, 66.25％, 79.93％ accordingly, and the volume, mass of tumor were decreased after treatment, in contrast to the saline group, the differences were significant (P<0.01). The tumor mass and volume were decreased in the test groups, more markedly in the combined drug group than single-drug groups. Moderate necrosis was found in tumor tissues.The expression of survivin mRNA and VEGF mRNA in the combination group was markedly lower than those in the other groups. The result of Western blot was also support RT-PCR's. Conclusion : Combined As2O3 and Vit C has stronger inhibition effect on tumor growth than single use of each drug, with a synergic anticancer effect on transplanted lung cancer in nude mice. Possible mechanism is related to the down-regulation of the expression of survivin and VEGF. Application of vitamin C and As2O3 might be a chemotherapy combination with low toxicity and efficient.关键词：arsenic trioxide;vitamin C;lung tumor;drug resistant;apoptosis;survivin;vascular endothelial growth factor
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Lifei Huaxian decoction (LFHX) on connective tissue growth factor (CTGF) and fibronectin (FN) signal transduction pathway in pulmonary fibrosis in rats. Method : Five randomized groups of Wistar rats were setup(n=20 each). Then experimental pulmonary fibrosis induced by bleomycin injection through tracheal was carried in the rats on day 0; treatments were ig given on day 1 by as prednisone acetate tablets 0.9 mg ·kg-1, LFHX 3.78 g ·kg-1, prednisone acetate-LFHX joint treatment, and normal saline in the control group and the model group. Ten animals in each group were randomly killed on day 7 and day 28 by bleeding through the aorta. The killing was carried out 2 h after last ig administration; blood sample was collected and lung tissue (right middle lobe) was kept in 4% neutral formaldehyde. Alveolitis and pulmonary fibrosis were evaluated with pathological slides stained by HE and Masson. Pulmonary fibrosis was measured by content of serum hydroxyproline (Hyp); CTGF, FN in lung tissue by immunohistochemistry. Result : Levels of alveolitis and pulmonary fibrosis, serum HYP, CTGF, FN of model group were significantly increased compared with those in control group (P<0.05); while the above indicators of treatment groups were significantly lower than those in model group (P<0.05). Conclusion : LFHX shows anti-pulmonary fibrosis action. It is likely related with CTGF, FN inhibition.关键词：Lifei Huaxian decoction;connective tissue growth factor;fibronectin;signal transduction pathway;pulmonary fibrosis;hydroxyproline
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发布时间：2024-01-04 - 摘要：Objective: To explore the relationship and mechanism about brown adipose tissue, uncoupling protein 1 (UCP1), high fatty diet and Chinese herbal decoction during the process of energy metabolism. Method : Rats were divided into four groups (n=16 each). ①Control group was fed with ordinary food, also ig given 0.9% NaCl 10 g ·kg-1 during d70-d98 daily. The ability of ISO deducing heat generating was tested on d98, and the curve of the rectal temperature was monitored, and the peak within two hours and the area under the curve were determined. ②Yang deficiency syndrome group: the same procedure was carried out as the control group except removing brown adipose tissue on d42. ③Spleen yang deficiency syndrome group: the same procedure was carried out as the yang deficiency syndrome group except of being fed with high fatty diet (including 83% ordinary diet,15% triglycerides, 2% cholesterol) from d49 to d98 and placed in 19 ℃ environment every other day.④Chinese herbal decoction group: the same procedure was carried out as the spleen yang deficiency syndrome group except giving 4 g ·kg-1 ig daily with Aconitum Lizhong decoction and the body weight was measured every week.The index of the weight growth rate, the peak of temperature and the area under the temperature curve were all investigated. The content of UCP1 was determined by using brown adipose tissue. Result: ①Weight growth rate: compared with yang deficiency syndrome group, weight growth rate in spleen yang deficiency syndrome group was lower(P<0.01); compared with spleen yang deficiency syndrome group, weight growth rate was higher in the Chinese herbal decoction group (P<0.01).②Temperature: compared with yang deficiency syndrome group, the temperature peak of heat generating in spleen yang deficiency syndrome group was higher(P<0.01); compared with spleen yang deficiency syndrome group, temperature peak of heat generating was higher in Chinese herbal decoction group (P<0.01). There was a positive correlation between weight growth rate and temperature peak of heat generating.③Area under the temperature curve: Compared with yang deficiency syndrome group, the area in spleen yang deficiency syndrome group was increased (P<0.01); compared with spleen yang deficiency syndrome group, the area was increased in Chinese herbal decoction group (P<0.01). There appeared a positive correlation between weight growth rate and area under the temperature curve. Compared with yang deficiency syndrome group and spleen yang deficiency syndrome group, Chinese herbal decoction group up-regulated content of UCP1, and the difference was highly significant (P<0.01). Conclusion :Chinese herbal decoction with function of heat generating and tonifying spleen is inclined to make high fatty diet turn into brown adipose tissue and advance its activity. Chinese herbal decoction with function of warming middle energizer and tonifying spleen can upgrade level and expression of UCP1 in brown adipose tissue, and then make brown adipose tissue decomposed by the mechanism of uncoupling with UCP1, finally dispelling coldness from the bodies in the rats with yang deficiency syndrome.关键词：spleen yang deficiency syndrome;brown adipose tissue;uncoupling protein 1;correlation
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发布时间：2024-01-04 - 摘要：Objective: To establish hyperlipidemia model of rats, to observe total saponins of Medicago sative(BD-TAS) on blood lipid metabolism in hyperlipidemia rats. Method : Sixty SD rats were randomly divided into six groups: normal control group (NC), high-lipid model group (HC), Xuezhikang capsule group, and BD-TAS group. All groups were induced with high fat diet for 8 weeks except NC, to establish hyperlipemia. NC and HC group were ig physiological saline, BD-TAS (4, 9, 12 g ·kg-1), Xuezhikang Capsule group(0.5 g ·kg-1). The contents of TC,TG,LDL-C,HDL-C and MDA were examined four times in the plasma after 0,2,4,6 weeks. Result : The result showed that with high fat diet after 8 weeks, compared with NC group, TC, TG, HDL-C and LDL-C were increased significantly (P < 0.05), so hyperlipidemia rat model was established successfully. BD-TAS of different dose groups, TC and TG in plasma were reduced after 2,4,6 weeks, compared with HC group (P<0.05). The level of TC in low-dose BD-TAS group was statistically significant compared with Xuezhikang group (P<0.05) and the levels of TC, TG in high-dose BD-TAS group were statistically significant compared with low-dose BD-TAS group (P<0.05). Conclusion : The levels of TC,TG and MDA decreased with the increased of BD-TAS dose, indicating a clear dose-effect relationship. BD-TAS is effective in modulating the blood lipid levels.关键词：Medicago sativa;total saponins;hyperlipidemia;blood lipid metabolism
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Heibu ointment on the expression of type I and type Ⅲ collagen and MMP-1 mRAN in the hypetrrophic scarof rabbit ear. Method : Twenty-four white rabbits were randomly divided into the normal control group,Heibu ointment treatment group and model group. Heibu ointment treatment group and model group were used to establish the models of hypertrophic scar in ears.After twenty-one days, Heibu ointment (1 g) was plastered on the hypetrrophic scar in Heibu ointment treatment group and on the skin in the normal control group once every three days for 56 days. The tissure material was respectively taken on the second, the fourth, the sixth and the eighth week, the expression of type I and type Ⅲ collagen and MMP-1 mRAN were detected by real time PCR(RT-PCR) technique. Result : Compare with the model group, the expression of type I collagen mRAN was obviously weaker (P<0.01), the expression of type Ⅲ collagen and MMP-1 mRAN was increased in the Heibu ointment treatment group(P<0.01). Conclusion : Heibu ointment could inhibit the expression of type I collagen mRAN and promote the expression of type Ⅲ collagen and MMP-1 mRAN, thus it could inhibit the proliferation of hypertrophic scar of the rabbit ear.This maybe one of the mechanisms that the Heibu ointment inhibit the proliferation of hypertrophic scar of rabbit ear.关键词：Heibu Ointment;hypertrophic scar of rabbit ear;type I and type Ⅲ collagen;MMP-1
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Uncariae Ramulus Cum Uncis on the conditioned place preference (CPP) induced by morphine in rats. Method : Conditioned place preference was established by morphine sc 15 mg ·kg-1 ·d-1 for 5 d. In the training time,two Uncariae Ramulus Cum Uncis groups (3,9 g ·kg-1,ig) were given Ramulus Uncariae cum Uncis after sc morphine 12 h later daily. The duration of staying in the White Compartment was recorded. Result : The 3 g ·kg-1 group could not influence morphine-dependent CPP; the 9 g ·kg-1 group could extinct to some extent morphine-dependent CPP. Conclusion : The result suggest that Uncariae Ramulus Cum Uncis could extinct to some extent morphine-dependent CPP and has the function of resisting psychological dependence.关键词：rat;morphine dependent;Uncariae Ramulus Cum Unci;conditioned place preference
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发布时间：2024-01-04 - 摘要：Objective: To establish S180 sarcoma model on the chick embryo chorioallantoic membrane (CAM) and to study its morphological and biological properties. Method : S180 cell was cultivated and implanted on the CAMs under sterile conditions. The factors affecting the implanted tumors and the morphological and biological properties of the tumors were studied. Result : S180 sarcoma model on the CAM was established. The implanted tumor was easy to grow on the CAM, and had a strong tendency of angiogenesis. When the number of inoculated tumor cells reached 5×107 in each egg, the tumor rate could reach 83.33%. Compared with the first day, the number of induced vessels on the second day was significantly different (P<0.05). On the fourth day after transplant it was greatly increased (P<0.001). Conclusion : Our findings suggest that this model is of easy duplication and simple operation, and can be widely used in the research of the biological properties for tumor angiogenesis and it provides an intuitive platform for the drug screening of anti-angiogenic.关键词：S180;chick chorioallantoic membrane;implanted tumor;tumor formation rate
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of different proportion and different dosage forms of the combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix on migraine in animal models. Method :One experimental migraine animal model was duplicated by abdominal injection of nitroglycerin(10 mg ·kg-1) once, the other was duplicated by subcutaneous injection of reserpine(0.4 mg ·kg-1) seven days. After model establishment, the animals were treated with the combination of Chuanxiong Rhizoma and Radix Angelicae Dahuricae, and the levels of 5-hydroxytryptamine (5-HT), 5-hydroxyindole acedic acid(5-HIAA) and calatonin gene related peptide(CGRP) were determined. Result : Compared with model group, the combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix (3.5 g ·kg-1 ·d-1)could raise the contents of 5-HT about 48.41%-73.18%,reduce the contents of CGRP about 38.02%-50.02% (P<0.01)in migraine rats, and the combination (5 g ·kg-1 ·d-1) could raise the contents of 5-HT about 36.60%-69.52% in migraine mouse (P<0.05,P<0.01). Conclusion : The combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix has the best effect when it was given by the form of powders. It could improve migraine symptoms by controlling single of neurotransmitter dopamine release and alleviating neurogenic inflammation.关键词：Chuanxiong Rhizoma;Angelicae Dahuricae Radix;animal model ofmigraine;5-hydroxytryptamine;calcitonin gene related peptide
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of ethanol extracts from Portulacae Herba on learning and memory performances in senile mice induced by D-gal, and its mechanisms of effects. Method : The mice were randomly divided into 4 groups:control group, model group and the ethanol extracts from Portulacae Herba(1.0,0.5 g ·kg-1) groups. The control group was given sc of isotonic NaCl in the cervical back part while the other all were given sc D-gal(1 g ·kg-1), once daily.The model was continuously established for 42 d, meanwhile medicine was given. Learning and memory performances were tested with step-through, water maze and Morris water maze test, and to determine the contents of malonaldehyde(MDA) and superoxide dismutase(SOD), and to determine the activity of glutathione peroxidase(GSH-Px) in brain tissue simultaneously. Result : Compared with the model mice, the ethanol extract from Portulacae Herbacould significantly prolong the latency,and reduced the error frequency. It(1 g · kg-1) could obviously shorten escape latency and pass frequency of 1 min erect position in the senile mice. It could obviously reduce the content of MDA in the senile mice. Conclusion : The ethanol extract from Portulacae Herbacan improves the learning and memory performances of senile mice, the mechanism may be related to decrease in content of MDA in brain tissue.关键词：Portulacae Herba;senile;learning and memory;malonaldehyde;superoxide dismutase;glutathione peroxidase;mouse
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Qingpeng plaster on the pruritus and eczema in guinea-pigs. Method : The pruritus threshold of all plaster groups (0.48,0.24,0.12 g ·kg-1, 3 d) of guinea-pigs caused by histamine phosphate was investigated. After causing eczema in guinea-pigs, the overall score was observed and measure the contents of interleukin-2,interlenkin-4,tumor necrosis factor-α(TNF-α),interferin(IFN-γ) in serum were measured, and histopathological changes in the back skin of the guinea-pigs were observed, and the amount of inflammatory cells in dermis was counted. Result : Low dose group and high dose group could extend the period of time on guinea-pig feet-licking significantly, and increase the pruritus threshold of histamine phosphate in guinea-pig. Compared to guinea-pigs with eczema, each drug group showed as the area reduction or subsiding of spiloplaxia, so that the components scale of the drug groups were lower than those of the models, and there were obvious difference between the drug groups and the module groups. Qingpeng plaster had little impact on the IL-2,TNF-α,IFN-γ in serum. Comparing to the model groups, the IL-4 of high and low dose groups was increased and showed difference obviously. Through histopathologic examination, each drug group showed pathological features in varying degrees, such as the improvement on the cornification of epidermis, the thickening of prickle-cell layer, and the extension of trochanterellus. Moreover, the amount of the inflammatory cells in dermis of drug groups decreased obviously compared with that of model groups, and there were significant differences between the drug groups and the model groups. Conclusion : Qingpeng plaster has anti-pruritic action and can treat eczema effectively. The mechanism of treating eczema maybe related to inhibition of the inflammatory cells and adjustment of the inflammatory factors.关键词：eczema;Qingpeng plaster;pruritus;cytokines
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发布时间：2024-01-04 - 摘要：Objective: To explore the mechanism of Qingong capsule on uterine hemorrhage after medicine induced abortion. Method : Induced abortion models were made with pregnant rats drenched with mifeprisrone and misoprostol. The level of estrogen in blood, contents of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) in uterus homogenate of the rats after medicine induced abortion in every group were measured and compared. The action of Qingong (1.9, 3.8 g ·kg-1 ·d-1) capsule on medicine induced abortion was observed. Result : The Estrogen content in uterus homogenate Qingong capsule group was obviously higher than that in model group, and the content of NO and NOS were obviously lower than those in mdoel group. Conclusion : The mechanism of Qingong capsule on uterine haemorrhagia after medicine induced abortion may be related with improving the level of estrogen in blood and reducing the contents of NO and NOS in uterus tissue, thus it has better preventive and therapeutic effects on vaginal bleeding after medical abortion.关键词：Qingong capsule;medicine induced abortion;estrogen;nitric oxide;nitric oxide synthase
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of berberine on the expressions of cell adhesion molecules in rats with middle cerebral artery occlusion (MCAO). Method : Tirty-six rats were randomly divided into 3 groups: model group, Sham-operated group and berberine group. We used transient rat MCAO model for brain ischemia. Berberine (30 mg ·kg-1) was given twice intraperitoneally: 15 min pre-occlusion and 2 h post-occlusion at the time of reperfusion. The expressions of ICAM-1 and P-selectin berberine were analyzed by immunohistochemistry method. Result : Berberine significantly decreased the infarct volume and the expressions of ICAM-1 and P-selectin(P<0.05). Conclusion : Berberine may protect the brain injury induced by ischemia-reperfusion through the immunoregulation to the inflammation reaction after brain ischemia.关键词：cerebral ischemia-reperfusion;berberine;interllular adhesion molecule;P-selectin
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical curative effect and safety of icterus due to divided into two groups,the treatment group(n=43) treated with the combination chronic cholestasis treated withpurgation dispersion purification harmonization. Method: Eightg-five patients with Icterus due to chronic cholestasis were selected. They were randomly of purgation dispersion purification harmonization and western medicine,the control group(n=42) with western medicine. Result: After four weeks treatment, the treatment group was superior to the control group with regard to total effective rate (90.70% vs 83.33%, P<0.05) and improvement of liver function including TBIL,ALT and γ-GT(P<0.05 or P<0.01). The treatment of purgation dispersion purification harmonization was effective in cleaning up remnant icterus andwithout significant side effects. After three months follow-up,the level of Tbil,ALT and γ-GT did not rebounded. Conclusion : It was effective of Using the combination of purgation dispersion purification harmonization and western medicine to treat icterus due to chronic cholestasis. This therapy could help to clean up remnant icterus and was safe.关键词：cholestasis;icterus;purgation dispersion purification harmonization;integrated Chinese and western medicine
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发布时间：2024-01-04 - 摘要：Objective: To study clinical efficacy of Qishen Qinggan Decoction combined with lamivadine for treatment of chronic hepatitis B(CHB). Method :Seventy-nine CHB patients were randomly divided into two groups,41 were involved in treatment group,38 were involved in control group.The treatment group were treated by Qishen Qinggan Decoction combined with Lamivadine,the control group was treated by lamivadine.Fifty-two weeks for a course of treatment.Serum level of HBV DNA,HBV-M,liver function were detected to compare the therapeutic effect. Result :Baseline characteristics were well balanced between two groups. The serum HBV DNA became undetectable(<1 000 copies/mL)in 92.68% of treatment group vs 76.31% of control group(P<0.05).The rates of serum HBeAg became negative between 2 groups, were 46.67% vs 20.69%(P<0.05). The rates of serum HBeAg seroconversion between 2 groups were 33.33% vs 17.24%. The rates of hepatitis B virus variation between 2 groups were 4.88% vs 10.52 %. Liver function(ALT,AST,TBil,ALb)was significantly improved in both groups(P<0.05, P<0.01). Conclusion :Qishen Qinggan Decoction combined with Lamivadine for the treatment of CHB has better virus responsion.关键词：Qishen Qinggan Decoction;lamivadine;chronic hepatitis B
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发布时间：2024-01-04 - 摘要：Objective: Observation Liuwei Dihuang soft capsule treat diabetes (kidney Yin deficiency syndrome) clinical curative effect. Method :Two hundred and thirty-six cases by stratified area group diabetes patients randomized, double-blind simulation, positive drug and placebo parallel control, multicenter clinical test method. Test points three groups, 4.75%more: 118 cases of, oral Liuwei Dihuang soft capsule (3 grain/times) + left to pill simulation medicine (9 g/times), 2 times per day, the morning 1 times; 0 case: oral group 1 ∶6 Liuwei Dihuang soft capsule simulation medicine (3 grain/times) + left to pill (9 g/times), 2 times per day, the morning 1 times; All cases of control group, oral Liuwei Dihuang soft capsule simulation medicine (3 grain/times) + left to pill simulation medicine (9 g/times), 2 times per day, the morning 1 times. All three groups of course of 8 weeks. Result :Three groups of patients after disease medication syndromes curative effect levels of comparative differences have statistically significant (P < 0.001). Blood sugar control efficacy effect similar, difference was statistically significant. After treatment, the most major clinical symptoms improved situation between three groups was statistically significant comparative differences. Three groups were some cases after treatment laboratory appears unusual clinically meaningful change, clinical doctors think'change and test drug is not a matter of. Conclusion :The three groups of patients after treatment efficacy of disease syndromes difference between the level of statistical significance (P<0.001); effect of glycemic control similar effect, the difference was not statistically significant; clinical symptoms after treatment, most of the major improvements in three differences between groups was statistically significant. Three groups after treatment in some cases laboratory tests are clinically significant abnormal changes in clinical doctors to determine 'change has nothing to do with the test drugs.关键词：diabetes;kidney Yin deficiency syndrome;traditional Chinese medicine therapy;Liuwei Dihuang soft capsule
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Shenmai injection for hemorheologyof patients with myocardial infarction. Method :Seventy-four patients with myocardial infarction in our hospital from June 2008 to December 2010 were selected as research object,and they were randomly divided into control group(routine treatment group)37 cases and observation group(Shenmai injection group)37 cases,then the total effective rate,incidence of adverse reactions and indexes of hemorheology of two groups were analyzed and compared. Result :The total effective rate of observation group was higher than that of control group,incidence of adverse reactions was lower than that of contro group,whole blood viscosity,whole blood reduced viscosity and plasma viscosity, hematocritwere all lower than those of control group,(P＜0.05 or P＜0.01). Conclusion :The influence of Shenmai injection for hemorheology of patients with myocardial infarction is better,and it is worthy of popularization and application.
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发布时间：2024-01-04 - 摘要：Objective: To study the clinical effect of integrared traditional Chinese and westen medicine treatment on children enuresis. Mothod :Fifty-six cases are clected and randpmly dived into two groups, 28 cases in the control group were treated with the way of meclofenoxate hydrochloride and anisodamine,at the same time with psychotherapy and bladder training; 28 cases in the treatment group were treated with Otheca mantidis in addition to those of the control group. Result : Compared with those in the control group 17 cases are cused,5 cases show notable effect,4 cases have effect,2 cases with no effect,the total effectiveness was 92.9%. Conclusion :Most of enuresis cases are functional and can be effectively treated by employing the method of combining traditional Chinese medicine and western medicine.关键词：integrared traditional Chinese and westen;children enuresis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Qiangxinning Jianji on plasma levels of tumornecrosis factor alpha (TNF-α),interleukin-6 (IL-6) in chronic heart failure (CHF) patients. Method :CHF patients were randomly divided into the treatment group and control group. Plasma levels of TNF-α and IL-6 were measured and NYHA class was evaluated at the 8th week of treatment. Result :NYHA class was markedly improved in two groups, it was significantly better in treatment group than those in the control group (P<0.05). As compared with the control group, in the treatment group; TNF-α and IL-6 were lower, the difference between groups and between before and after treatment were significant (P<0.05). Conclusion :Qiangxinning Jianji could lower plasma levels of TNF-α and IL-6, and is one of the effective drugs in improving cardiac function in patients with CHF.关键词：Qiangxinning Jianji;chronic heart failure (CHF);tumor necrosis factor alpha (TNF-α);interleukin-6 (IL-6)
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发布时间：2024-01-04 - 摘要：Objective: To investigate the safety and efficacy of sublingual immunotherapy(SLIT)with Dermatophagoides Farinae Drops in the treatment of allergic rhinitis. Method :A randomized, placebo-controlled design was followed. A total of 55 children aged 3-14 years suffering from allergic rhinitis due to dermatophagoides farinae(according to the symptom of allergic rhinitis and conformed by Skin prick tests) were subdivided into two matched groups:SLIT+Drug-group and Drug-group. SLIT+Drug-group:34 underwent sublingual standardized Dermatophagoides Farinae Drops plus drugs. Drug-group: 21 received only drug therapy such as mometasone furoate aqueous nasal spray, loratadine tablets.The patients underwent a timing control visit of once every 2 months and lasted 6 months. Result : SLIT+Drug-group showed a significant reduction of symptom scores(P<0.05) and medication scores(P<0.05) when compared with Drug-group,and the total effective rate of SLIT+Drug-group was 97.06%. Adverse reactions were observed in 3 children in SLIT+Drug-group(8.8% of patients). No severe systemic reactions were reported. Conclusion : SLIT with standardized Dermatophagoides Farinae Drops provided benefits in mite-allergic children in terms of significantly reduced symptoms and medication use. SLIT was safe and effective in children aged3-14years with allergic rhinitis.关键词：allergic rhinitis;sublingual immunotherapy;Dermatophagoides Farinae Drops
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发布时间：2024-01-04 - 摘要：Objective: To observe the efficacy and safety of Habitat Strychnos pill Combined with leflunomide for the treatment of rheumatoid arthritis (RA). Method: Ninety cases of RA were randomly divided into 2 groups, control group(40 cases) given leflunomide with oral, as well as symptomatic treatment; the treatment group (50 cases)received oral Chinese medicine Habitat Strychnos pill on the basis of therapy in the control group. The course of treatment was 6 months. Before and after treatment changes in indicators of clinical efficacy and adverse drug reactions were monitored. Result: The treatment group was significantly higher than the control group for the total effective rate (P<0.05). Compared with pre-treatment, the treatment significantly improved efficacy in the number of joint pain, joint tenderness score, joint swelling score, morning stiffness time and 20 m walking time, ESR,CRP (P<0.05 or P <0.01). Oral medicine pills habitat Strychnos did not show obvious side effects. Conclusion :Habitat Strychnos pill with leflunomide for the treatment of RA is more effective than leflunomide alone, and the safety is good.关键词：rheumatoid arthritis;Chinese medicine Habitat Strychnos pill;leflunomide
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发布时间：2024-01-04 - 摘要：Objective: Tp investigate the clinical efficacy of Tongmai Zhitong Fang and nimodipine in the treatment of migraine. Method :Sixty-two migraine patients were randomly divided into treatment group (n=40) and control group (n=22), the control group were treated with nimodipine 40 mg, 3 times a day, orally, 4 weeks for a course of treatment. Based on the use of nimodipine in the control, patients in the treatment group were treated with Tongmai Zhitong Fang additionally (one dose divided in two times daily).Before and after treatment, the total effective rate and the maximum blood flow velocity (Vs) of bilateral middle cerebral artery (MCA) in that two groups were compared. Result :The total effective rate was 85% in the treatment group, total effective rate was 63.6% in the control group, the difference was statistically significant (P<0.05). After treatment, in the two groups Vs were significantly decreased, compared with that before treatment the difference was significant (P<0.01), the effect in treatment group was more obvious (P<0.01). Conclusion :Tongmai Zhitong Fang combined with nimodipine has significant effect for the treatment of migraine.关键词：Tongmai Zhitong Fang;nimodipine;migraine
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发布时间：2024-01-04 - 摘要：To review the research methods of pharmacokinetics of traditional Chinese medicine(TCM) and detect analysis methods in recent years. Papers reported in journals were consulted and classified, according to the domestic and overseas literatures over 30 papers. The blood medicine density is the main study method of pharmacokinetics of TCM, still including syndrome and treatment pharmacokinetics, serum pharmacology of TCM, gastrointestinal dynamics of TCM, modeling of pharmacokinetic-pharmacodynamic (PK-PD) and so on. The Liquid Chromatography(LC) is the substantial methods of detect analysis and hyphenated techniques chromatography(HTC) has founded new thoughts to studying the pharmacokinetics of TCM. The pharmacokinetics of TCM has already begun to take scale, but it is concentrate on study of single compounds of TCM. Future researches should focus on explore the metabolism process and function mechanism of traditional chinese prescription to seek better research approaches.关键词：pharmacokinetics of traditional Chinese medicine;biological sample treatment;instrumental analysis
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发布时间：2024-01-04 - 摘要：The theory of intranasal brain-targeting system and its preparations were sumarized to provide basis for traditional Chinese mediline (TCM). literatures searching were based on keywords as intranasal administration, nasal administration and brain-targeting system. The paper not only described the nasal cavity physiological characteristics to study the transport mechanisms of intranasal drug delivery for brain targeting, but also reviewed the situation of the central nerve system disease treatment using the intranasal drug delivery for brain targeting. The paper also described the new intranasal formulation (in situ gel, liposomes, microspheres, nanoparticles formulations)and traditional Chinese medicine nasal administration to understand the process of traditional Chinese medicine nasal administration. Intranasal brain-targeting preparations of TCM had broad development prospect.关键词：intranasal drug delivery;blood-brain barrier;brain targeting;central nervous system
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发布时间：2024-01-04 - 摘要：Due to its multifunctional properties, it generated much attention in both the communities of research and application. To review the progress on effect of gardeniae Fructus(geniposide, genipin, crocin, crocetin) on cardiovascular system, so as to provide more information on gardeniae Fructus for further research and application. The literatures published home and abroad in recent years were consulted and summarized. Modern studies show that gardeniae Fructus not only have protective effects on endothelial cells, antioxygen, anti-inflammatory, anticoagulation and other effects, but also have protective effects against smooth muscle cells proliferation. Currently there are more studies about gardeniae Fructus iridoid glycosides against ischemic injury, and the mechanism is multifaceted. More studies are needed on the mechanism of gardeniae Fructus for treatment of cardiovascular system injury. This review expects to provide reference for preventing atherosclerosis and developing new drugs.关键词：Gardeniae Fructus;geniposide;angiocellular;anti-inflammatory;cardiovascular system
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发布时间：2024-01-04 - 摘要：Acute soft tissue injury animal models and experimental indicators of the collation and analysis of the literature. Collection of relevant literature and conduct search to sum up. Acute soft tissue injury animal model commonly used beat method,the impact method,injection,compression method and the cutting method.Experimental studies in a variety of experimental observations are mostly concentrated in selected indicators of the general macro-observation,analgesic and anti-inflammatory effects of swelling and microscopic histological,biochemical factors,the determination of blood rheology. With modern molecular biology and the development of molecular immunology,molecular and gene levels in the future will be the external application of Chinese medicine treatment of acute soft tissue animal research direction.
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发布时间：2024-01-04 - 摘要：The article reviewed the research on in vivo process of protoberberine alkaloids by oral administration,aimed to provide reference data for the choice of dosage form, research of pharmacodynamics and toxicology, and rational use of protoberberine alkaloids. By literatrue retrieval in China Knowledge Resource Integrated Database, VIP technology journal full-text database and Foreign Medical Journal Full-Text Service,the article reviewed the progress of the gastrointestinal absorption, tissue distribution, metabolic pathway and product,excretion and the pharmacokinetic parameters of protoberberine alkaloids by oral administration. Researches of several active ingredients involved intestinal ransport mechanism and characteristic, tissue distribution, metabolism and excretion, main metabolites, orally dynamic parameters etc. In vivo process of protoberberine alkaloids by oral administration is relatively complex. Current research is not systematic enough. Drug interactions of in-vivo process is unclear, pharmacological effects of metabolites still need further confirmation.关键词：protoberberine alkaloids;absorption;distribution;metabolism;excretion;pharmacokinetics
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发布时间：2024-01-04 - 摘要：The literatures on medicine and herbs from ancient time to modern time were collected and studied, and the historical changes of methods processing Fluoritum and the survey of processed in different provinces were summarized. Discusses the original mineral traditional Chinese medicine purple quartz source. Since the Fluoritum first record in "Shengnong Bencaojin" in Han dynasty, after the traditional heritage and development, and sums up the modern purple quartz drug theory. Modern research put forward for the Chinese medicine of the original mineral sources purple quartz for mineral fluorite (CaF2), this is the source of the purple quartz textual research and quality standard provided valuable information. The different processing methods of the change in the composition of the chemical, and puts forward the optimal processing conditions. Summarizes the determination of different traditional Chinese medicine in the content of purple quartz method. Record the Chinese traditional medicine inclinical application of purple quartz various therapeutic and calendar version of China pharmacopoeia.关键词：fluoritum;mineral fluorite;herbal research;CaF2
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发布时间：2024-01-04
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