最新刊期
卷 18 ， 期 23 ， 2012
- 摘要：Objective: To classify the herbs for the treatment of cancer pain. Method: Database of 25 commonly used herbs for was established by means of SPSS 11.5 software package; and the nature, tastes, meridian attributions and actions of the herbs were collected as variables showing the characteristics of classification, and the names of the herbs served as variables for measurement to proceed Q Cluster in Hierarchical Cluster. Result: The characteristic of the herb for the treatment of cancer pain is pungent, bitter, warm and the correspondent channels are liver and spleen. They can clear heat and detoxify, activate blood to relieve pain. Conclusion: Fuzzy grouping analysis can be applied to classify herbs, and can be guiding the clinical medication.关键词：cancer pain;fuzzy grouping analysis;quantitative;classification
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发布时间：2024-01-04 - 摘要：Objective: To analyse the effective combination drugs of GAN Zu-wang formulae in preventing and treating the ear disease. Method: One hundred and one formulas were selected from the public documents about professor GAN Zu-wang,the fuzzy clustering and frequent pattern tree algorithm were used to analyse the core medicine groups and the basic formulas by similarity matching in medicine efficacy and composition. Result: One hundred and one prescriptions were divided into 9 medicine groups,11 basic formulas were recognized, and the mainly formulas were those for regulating spleen-stomach and benefiting qi-xie such as Bu-zhong-yi-qi-tang, Si-jun-zi-tang and Ba-zheng-tang. Conclusion: Regulating spleen and stomach was professor GAN Zu-wang’s specialized treatment experience for ear disease,the method of data mining could provide interesting rules for understanding the academic thoughts of the famous and respected doctor of traditional Chinese medicine.关键词：fuzzy clustering analysis;frequent pattern tree;professor GAN Zu-wang;specialized treatment experience for ear disease
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation technology of β-cyclodextrin inclusion compound of volatile oil from Baizhishan cold granule. Method: With inclusion rate of volatile oil,yield and oil ratio of inclusion complex as indexes,optimum technology was selected from saturated aqueous solution method,ultrasonic method and trituration method;With inclusion rate of volatile oil and yield of inclusion complex as comprehensive evaluation indexes, orthogonal design was used to optimize inclusion technology of volatile oil with ratio of volatile oil-β-CD,inclusion temperature,inclusion time and proportion of water-β-CD as factors;Phase of this prepared inclusion complex was testified by analyses of TLC,UV and microscopic imaging. Result: The best method of inclusion was saturated aqueous solution method,and optimum inclusion technology conditions were as follows:ratio of volatile oil-β-CD 1∶8,inclusion temperature of 55 ℃,mixing time of 3 h,β-CD-water ratio of 1∶10.Analysis results of TLC,UV and microscopic imaging showed that stable inclusion compound has been formed from volatile oil and β-CD. Conclusion: This optimized technology was simple,stable and feasible,it could be suitable for large-scale production.关键词：Baizhishan cold granule;volatile oil;β-cyclodextrin inclusion complex;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To study factors affecting stability of β-CD inclusion compound of volatile oil from traditional Chinese medicine,and compare stability difference from inclusion compounds which prepared by two methods. Method: With relative volatile oil loss rate as index,single factor test was used to explore stability under these conditions,such as temperature of 60 ℃, light of (4 500± 500) lx and humidity of 75%.Volatile oil in inclusion compound was extracted by steam distillation method,composition change was detected by GC-MS between before and after stability test. Result: After inclusion of volatile oil,its stability to light,heat and humidity had significantly improved,3 kinds of factors affecting relative volatile oil loss rate were in order of high humidity>high temperature>strong light;Three factors had greater effect on composition in volatile oil inclusion with heavy gravity and high refractive index than that of small gravity and low refractive index. Conclusion: Inclusion prepared by colloid mill method has better stability on volatile oil content and composition than saturated aqueous solution method.关键词：volatile oil;inclusion compound;stability;influencing factor
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发布时间：2024-01-04 - 摘要：Objective: To investigate solubilization effect of compatibility of Glycyrrhiza uralensis-Baphicacanthus cusia. Method: Compatibility of G. uralensis and B. cusia,glycyrrhizic acid and indigo(indirubin),citric acid and B. cusia,the content of indigo and indirubin in samples were determined by HPLC. Result: When B. cusia was compatible with raw and fried G. uralensis,the content of indigo and indirubin in solution were increased significantly,but they were about 30% lower in fired G. uralensis than raw one;glycyrrhizic acid ammonium salt mixed directly with indigo and indirubin respectively by proportion,the content of them had significantly increased, with improving about 4~8 times, the best proportion was 2∶1;different proportion of citric acid was compatible with B. cusia,the content of indigo and indirubin in solution showed no significant change. Conclusion: Compatibility of B. cusia and G. uralensis could achieve purpose of solubilization,glycyrrhizic acid played a major role,it could provide a theoretical basis for compatibility application of TCM.关键词：Baphicacanthus cusia;indigo;Glycyrrhiza uralensis;glycyrrhizic-acid;compa-tibility solubilization
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发布时间：2024-01-04 - 摘要：Objective: To optimize high pressure steaming process of Aconitum carmichaelii. Method: Orthogonal test was used to optimize high pressure steaming process of A. carmichaelii with appearance quality,the content of diester diterpenoid alkaloids and total alkaloid as comprehensive evaluation index,high pressure steaming time,pressure and softening way were selected as factors. Result: Optimum high pressure steaming technology was A2B2C2,that of treated by wetting method,steamed 150 min at 0.10 MPa. Conclusion: This optimized process was simple,practical and easy to control,it could be used as a new method to replace traditional processing technology of A. carmichaelii.关键词：Aconitum carmichaelii;high pressure steaming;orthogonal design;diester diterpenoid alkaloids;monoester alkaloid;total alkaloid
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发布时间：2024-01-04 - 摘要：Objective: To optimize water boiling process of Buyang Huanwu decoction. Method: With the content of astragaloside and ferulic acid as indexes,orthogonal test was used to optimized water boiling technology of Buyang Huanwu decoction with soaking time,boiling time,boiling times and concentrated volume as factors. Result: The best water decoction technology was:soaked 30 min with 10 folds water,boiled 2 times with 30 min per time. Conclusion: Optimzed technology was reasonable,feasible and reproducible,it could be applied in mass production of Buyang Huanwu decoction.关键词：Buyang Huanwu Decoction;astragaloside;ferulic acid;water boiling process;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasonic extraction technology of procyanidins from seed of Osmanthus fragrans. Method: With yield of procyanidins as index,extraction time, extraction temperature, solid-liquid ratio and the concentration of ethanol were chosen as factors,based on single factor test,ultrasonic extraction technology was optimized by orthogonal test. Result: Optimum extraction technology was:extracted 25 min with 8 times the amount of 50% ethanol at 50 ℃. Conclusion: This optimized extraction process was quick and simple, and it could provide experimental basis for development and utilization of seed of O. fragrans.关键词：seed of Osmanthus fragrans;procyanidins;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To prepare o/w type emulsion with total coumarin from Cnidium monnieri,and investigate its in vitro anti-trichomonas vaginalis activity. Method: Total coumarin from C. monnieri was extracted by ultrasonic method with emulsification time,times and pressure,adding order of emulsifier as factors,single factor test was used to optimize preparation technology of C. monnieri emulsion,and its pharmacodynamic trial of in vitro anti-trichomonas vaginalis was investigated. Result: Preparation technology of C. monnieri emulsion was ascertained as follows:emulsification 3 times at 150 MPa,15 min each time,adding order of emulsifier with oil phase added to aqueous phase;and this emulsion had good inhibition on trichomonas vaginalis. Conclusion: This preparation technology was stable and feasible,minimum drug concentration of this emulsion had good inhibition effect to trichomonas vaginalis was 0.15-0.62 g·L-1.关键词：osthol;emulsion;anti-trichomonas vaginalis
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发布时间：2024-01-04 - 摘要：Objective: To explore extraction process conditions of total flavonoid in Selaginella uncinata by pectinase enzymatic method. Method: With extraction ratio of total flavonoid as index,L9 (34) orthogonal test was token to optimize extraction technology of total flavonoid with pectinase dosage,enzymatic hydrolysis time,pH and enzymatic hydrolysis temperature as factors.The content of total flavonoid was determined by UV. Result: Optimum extraction technology conditions were as follows:the concentration of pectinase 0.075 g·L-1,enzymatic hydrolysis time 2.5 h,hydrolysis pH 5.0,extraction temperature 50 ℃,under these conditions,average extraction ratio of total flavonoid 1.40%. Conclusion: Optimized extraction technology was stable and feasible with high extraction efficiency,it provided experimental basis for further development of S. uncinata.关键词：Selaginella uncinata;total flavonoid;pectinase;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total flavonoids from Viscum ovalifolium,and to preliminary evaluate in vitro inhibitory effect of total flavonoids on human hepatoma cell Hep3B. Method: With rutin as standard substance,the content of total flavonoids was determined by UV,orthogonal test was used to optimize extraction technology of total flavonoid from V. ovalifolium with extraction time,extraction temperature,solid-liquid ratio and the concentration of ethanol as factors,extraction times was investigated by single factor test;In vitro inhibition effect of total flavonoids on Hep3B was measured by MTT method. Result: Optimum extraction technology was:extracted 2 times with 30 times the amount of 60% ethanol at 80 ℃,2 h per time;In vitro anti-tumor activity screening showed,when treated with the concentration of total flavonoid 500 mg·L-1,rate of growth inhibition on Hep3B was 51.40%. Conclusion: Optimized extraction conditions was stable and feasible,total flavonoids from V. ovalifolium showed some in vitro inhibitory effect on Hep3B.关键词：Viscum ovalifolium;total flavonoids;extraction process;anti-tumor
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Zhongfeng Huoxin soft capsule. Method: Volatile oil from Zhongfeng Huoxin prescription was extracted by steam distillation method,with yield of volatile oil as index,orthogonal test was adopted to optimize extraction technology with the amount of water,soaking time and distillation time as factors;With the content of puerarin as index,the amount of water,boiling time and extraction times were selected as factors,extraction technology of Zhongfeng Huoxin soft capsule was optimized by orthogonal test. Result: The best extraction technology of volatile oil was as follows:without soaking,distilled 7 h with 6 times the amount of water;optimum water extraction process of Zhongfeng Huoxin soft capsule was as follows:extracted 3 times with 10 times the amount of water,1.0 h each time. Conclusion: This optimized extraction technology was stable and reliable,it could be extended to large-scale production application of Zhongfeng Huoxin soft capsule.关键词：orthogonal test;extraction process;stroke;volatile oil;puerarin
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发布时间：2024-01-04 - 摘要：Objective: To optimize extracting process of total alkaloids from Zanthoxylum nitidum by enzymatic hydrolysis assisted immersion. Method: With extraction rate of total alkaloids as index,effect of 5 factors on enzymatic technology was investigated by single factor test,including kind of enzyme,enzyme dosage,pH, temperature and time;extraction times,extraction time and the concentration of ethanol were chosen as factors,effects of 3 factors on immersion technology of total alkaloids from Z. nitidum was optimized by orthogonal test. Result: Optimized enzymatic hydrolysis process was as following:cellulase-substrate and pectinase-substrate of 1∶250, 4 times buffer with pH 5, enzyme pretreating for 30 min at ambient temperature (30 ℃);Optimum immersion extracting technology was following:extracted 3 times with 60% ethanol of 12,4, 3 times amount(hydrochloric acid of 5 g·L-1 ), at ambient temperature,2 h each time. Extraction rate of total alkaloids of 85.22% by this process. Conclusion: This optimized process was simple,efficient,energy saving and environmentally friendly,and it could provide experimental base for industrial production.关键词：Zanthoxylum nitidum;total alkaloids;enzymatic;orthogonal test;immersion
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发布时间：2024-01-04 - 摘要：Objective: To study on synchronous release of gastrodin and turpentine alcohot-biglucosid from Tianma Gouteng decoction sustained release tablet,and provide experimental basis for formulation reform of Tianma Gouteng decoction. Method: In vitro dissolution was investigated by rotating basket method,the content of gastrodin and turpentine alcohot-biglucosid were determined by HPLC,similar factor was adopted for comparing similarity of drug release curve by calculating cumulative dissolution ratio of medicinal components. Result: Gastrodin and turpentine alcohot-biglucosid could suatained relese 12 h in vitro,and had synchronization. Conclusion: Tianma Gouteng decoction sustained release tablet had dissolution characteristics of sustained release,release of gastrodin and turpentine alcohot-biglucosid had similarity.关键词：Tianma Gouteng decoction;sustained release tablet;gastrodin;turpentine alcohot-biglucosid;synchronous release
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发布时间：2024-01-04 - 摘要：Objective: To optimize the formulation and process development of Trollius chinensis dispersible tablets. Method: With the amount of microcrystalline cellulose (A), low replace hydroxypropyl cellulose (B), crosslinking povidone (C) and aerosol(D) as the influencing factors and the disintegrating time taken as index, the formula of Trollius chinensis dispersible tablets was optimized by orthogonal experiment; the content determination was done by HPLC. Result: The optimized formula was A3B2C3D3.The contents of A,B,C and D were 30%,5%,8% and 8%, respectively. The products formulated by the optimum techniques were bright and clean, the average disintegration time was 80 s with good distribution homogeneity. The content of orientin was 16.02 mg/piece and the dissolution quantity achieved 90% above in 15 min. Conclusion: The dispersible tablets prepared in this study are better and have a good distribution homogeneity, it can reach the design standard.
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发布时间：2024-01-04 - 摘要：Objective: To optimize deproteinized technology of polysaccharide from Litchi chinensis. Method: With retention rate and deproteinized rate of polysaccharide as comprehensive evaluation indexes,the amount of enzyme,enzymatic time,pH and temperature were selected as factors,deproteinized technology of polysaccharide from L. chinensis was optimized by orthogonal test with papain;With retention rate and deproteinized rate of polysaccharide as index,deproteinized effect of six methods including papain, Sevag, TCA, Enzyme-Sevag, Enzyme-TCA and TCA-Sevag on polysaccharide from L. chinensis were compared. Result: Optimum deproteinized technology of papain was:the amount of papain 4%,time 1 h,pH 6,temperature 45 ℃,retention rate and deproteinized rate of polysaccharide were 82.9%,70.2%,respectively;Enzyme-Sevag method had optimum deproteinized effect,basis on enzymatic method,processed 2 times with Sevag,retention rate of polysaccharides was 71.42%,and deproteinized rate was up to 91.45%. Conclusion: Deproteinized effect on polysaccharide from L. chinensis had significant difference by different deproteinized method,enzyme-Sevag combined method had optimum deproteinized effect,it could be extended to application of large-scale production.关键词：Litchi chinensis;polysaccharide;deproteinized;enzyme-Sevag combined method
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total flavonoid from Malus hupehensis by entropy-weighting method. Method: With yield of extract,the content of total flavonoid and phloridzin as comprehensive evaluation indexes,the concentration and dosage of solvent,extraction time were selected as factors,weight of which were determined by entropy-weighting method,extraction technology was optimized by orthogonal test which was realized by Matlab. Result: Optimum extraction technology was:reflux extracted 3 times with 10 folds the amount of 70% ethanol,1 h each time. Conclusion: Entropy-weighting method could be used in optimizing of extraction technology for traditional Chinese medicine,calculation amount of orthogonal test could be reduced by Matlab programming.关键词：Malus hupehensis;entropy-weighting;comprehensive evaluation;total flavonoid;MATLAB
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发布时间：2024-01-04 - 摘要：Objective: To establish the assay method for determining content of homoplantaginin in extract of Salvia plebeia. Method : Hedera ODS-2 column was used with methanol-0.5% acetic acid (50∶50) as mobile phase; the column temperature was maintained at 30 ℃; the flow rate was 1 mL·min-1; detection wavelength was set at 335 nm. Result: Homoplantaginin showed a good linear relationship within 0.20-2.01 μg. The average of coefficient of recovery was 101.70%, RSD was 1.84%. Conclusion: This method is simple, accurate, specific, and can be considered as an effective method for determining content of homoplantaginin in the extract of Salvia plebeia.关键词：Salvia plebeia extract;HPLC;homoplantaginin;content determination
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of the paeoniflorin in Qianlie Antong capsule by HPLC. Method: The separation was performed on Waters Nova-Pak C18 (4.6 mm×150 mm,4 μm)column eluted with the mobile phase consisting of acetonitrile-water (15∶85) at the flow rate of 1mL·min-1.The detection wavelength was at 230 nm and the retention time of paeoniforin was 3.757 min. Result: Paeoniflorin showed good linearity(r=0.997 6)in the range of 0.20-4.0 μg.The mean recoveries of low,middle and high concentration of the components were among 97.85%-100.38% (RSD 0.37%-0.81%). Conclusion: The method is rapid,simple,accurate and reliable,and it can be used for determining paeoniflorin in Qianlie Antong capsule.关键词：HPLC;Qianlie Antong capsule;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard for Cuyun mixture. Method: Radix Astragali Mongolici, Radix Scutellariae Baicalensis and Radix Dipsaci in this prescription were identified by TLC; and the content of astragaloside Ⅳ in Cuyun mixture was determined by HPLC. The column of Kromasil C18 (4.6 mm×250 mm, 5 μm) was used; the mobile phase consisted of acetonitrile-water (35∶65); the flow rate was 0.8 mL·min-1. Result: TLC spots were clear with strong specificity and easy identification. While the linear range of astragaloside Ⅳ was 1.02-10.2 μg(r=0.999 7), the average recovery rate was 99.96%, RSD 1.01% (n=6). Conclusion: The method is easy, accurate and reproducible. It can be used effectively for the quality control of this preparation.关键词：Cuyun mixture;astragaloside Ⅳ;HPLC;quality standard
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components of the volatile oil from the roots of Rhodiola crenulata. Method: The volatile oil was extracted by steam distillation, the components were identified by GC-MS and their amount were determined by area normalization. Result: 72 kinds of substances were isolated and 52 compounds were identified representing 84.79% of the total distillate composition. The major constituents were geraniol (58.24%), 2-pentanone,4-cyclohexylidene-3,3-diethyl-(3.45%), geranyl acetate (3.04%), 1-methyl-4-(2-methyloxiranyl)-7-oxabicyclo [4.0.1] heptanes (2.04%), squalene (1.73%), 8-hydroxylinalool (1.16%). Conclusion: The determination of chemical constituents of volatile oil from roots of R. crenulata will provide important foundation for further exploitation and quality identification of R. crenulata.关键词：Rhodiola crenulata;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for simultaneous determination of vanillic acid and protocatechuic acid in Capparis spinosa. Method: The samples were separated on a C18 column (4.6 mm×150 mm, 5 μm), the mobile phase was consisted of methanol-0.1% phosphoric acid with gradient elution at the flow rate of 0.8 mL·min-1 and the detection wavelength was set at 258 nm. Result: The calibration curves for vanillic acid and protocatechuic acid were linear in the range of 0.001 50-0.009 00,0.005 80-0.034 8 μg respectively. All the correlation coefficient were 0.999 9, and the average recoveries (n=3) were 105% and 103% with corresponding RSDs of 1.90% and 1.81% respectively. Conclusion: The method is simple, accurate and sensitive and can be used to determine the content of the vanillic acid and protocatechuic acid in C. spinosa.关键词：Capparis spinosa;HPLC;vanillic acid;protocatechuic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for fingerprint analysis of olibanum from different sources by high-performance thin-layer chromatography(HPTLC). Method: The samples were extracted by ultrasonic with menthol. Chromatography was performed on silica gel 60F254 HPTLC plate, using toluene-ethyl acetate-heptanes-anhydrous formic acid (8∶2∶1∶0.3) as mobile phase, examined and recorded in ultraviolet light at 254 nm firstly, then in white light after derivatization with anisaldehyde test solution. The images were converted to grayscale intensity digital profile and then generated common pattern of HPTLC fingerprints with CHROMAP software, and authentication and quality assessment were analyzed by similarity analysis, cluster analysis and principal component analysis. Result: Twelve bands present in the chromatogram of Ethiopian olibanum in white light. There was notable difference between Ethiopian pattern and Somalian pattern. Olibanum from different species could readily be distinguished from each other so that commercial samples can easily be classified. Conclusion: The method is simple, specific and accurate, can be used for detection and quality control of olibanum.关键词：high-performance thin layer chromatography (HPTLC);fingerprint;chemometrics;digital profile;boswellia;olibanum (frankincense);boswellic acids
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standards for Slurry of Xiaobaidu. Method: The thin-layer chromatography was adopted for qualitative identification. Paeoniflorin was detected by HPLC. Result: The determination method for the content of paeoniflorin was established. The calibration curve of paeoniflorin was linear in the range of 0.182 4-3.648 0 μg. The average recovery of paeoniflorin was 98.4%. Conclusion: The method is simple, accurate and reproducible. It can be used for the quality control of compound Xiaobaidu Slurry.关键词：Xiaobaidu Slurry;TLC;HPLC;paeoniflorin;quality standard
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 in Mingmu granule. Method: HPLC was used,Dikma Diamonsil C18 column (4.6 mm×150 mm, 5μm),mobile phase of acetonitrile and water with gradient elution,flow rate was 1.0 mL·min-1 and detection wavelength was at 203 nm,column temperature was 25 ℃,injection volume 10 μL. Result: Linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.352-2.112(r=0.999 3),0.914-5.484(r=0.999 2),0.910-5.460 μg (r=0.999 1), respectively.Their average recoveries were 99.4% (RSD 2.56%),102.72% (RSD 2.16%) and 101.89% (RSD 2.16%), respectively. Conclusion: This established determination method was simple,accurate and reproducible,thus it could be used as quality control of Mingmu granule.关键词：Mingmu granule;HPLC;notoginsenoside R1;Ginsenoside Rg1;ginsenoside Rb1
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发布时间：2024-01-04 - 摘要：Objective: To establish a method to determine the entrapment efficiency of oleanolic acid liposomes. Method: Oleanolic acid liposomes were prepared by film dispersion method. Entrapment efficiency was determined after the separation of the unentrapped drug by glucan gel column chromatography. Distilled water was used to elute liposomes, 0.5% SLS was used to elute unentrapped drug. The content of oleanolic acid was deternined by HPLC. Result: The linear range of oleanolic acid was 0.302-30.2 mg·L-1(r=0.999 9).The average recovery was 99.92% (RSD 1.59%). The recovery of glucan gel column chromatography was 100.22% (RSD 1.23%), and the recovery of oleanolic acid was 99.04% (RSD 0.99%). The average entrapment efficiency of oleanolic acid liposomes was 83.4%. Conclusion: This method is accurate, credible and it can be used to determine the entrapment efficiency of oleanolic acid liposomes.关键词：oleanolic acid;liposomes;entrapment efficiency;glucan gel column chromatography;HPLC
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发布时间：2024-01-04 - 摘要：Objective: A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of 17β-tenacigenin B and tenacigenoside A in Xiaoaiping injection. Method: The analytical column was Agilent Zorbax SB-C18 (3.0 mm×100 mm, 3.5 μm) and the security guard column was Agilent Zorbax SB-C18 (2.1 mm×12.5 mm, 5 μm). The mobile phase was methanol-water (90∶10) at a flow rate of 0.25 mL·min-1. Methyltestosterone was used as internal standard; electrospray ionization model (ESI) and SIM model were used for quantification. Result: The linear ranges of two compounds were as follows: 0.625-20 mg·L-1 (r=0.998 3) for 17β-tenacigenin B, 0.375-12 mg·L-1 (r=0.999 4) for tenacigenoside A. The average recoveries were 100.8% (RSD 3.95%), 101.6% (RSD 2.71%), respectively. Conclusion: This established method was sensitive, fast, accurate and available for the quality control of Xiaoaiping Injection.关键词：Xiaoaiping injection;17β-tenacigenin B;tenacigenoside A;simultaneous determination
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发布时间：2024-01-04 - 摘要：Objective: To study volatile components of Marsdenia tenocissima. Method: Soxhlet extraction method with petroleum ether and ethyl ether were accompanied with gas chromatography mass spectrometry (GC-MS) to analyze the volatile components respectively. Result: 24 compounds identified by GC-MS with petroleum ether accounted for 55.43% of all volatile components; fatty acids were 75.10% and alkanes were 11.91%; 24 compounds identified with ethyl ether accounted for 60.43%; fatty acids were 38.98% and alkanes were 39.27%. Conclusion: The study provides useful data for further analysis of components of Marsdenia tenocissima.关键词：Marsdenia tenocissima;GC-MS;Soxhlet extraction method
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发布时间：2024-01-04 - 摘要：Objective: To revise and improve the quality standard of vinegar backed E. ebracteolata. Method: 10 batches of vinegar backed Euphorbia ebracteolata were investigated with TLC. Moisture content, total ash, acid-insoluble ash and extractum were explored. The content of ebracteolata cpd B was determined by HPLC. Result: The quality standard was preliminarily formulated as follows: the moisture content should not pass 9.12%. Total ash should not pass 6.50%. Acid-insoluble ash should not pass 1.09%. Alcohol-soluble extractive should not lower than 7.70%. The content of ebracteolata cpd B should not lower than 0.038%. Conclusion: Quality control quantization evaluation system of processed E. ebracteolata slice is preliminarily established.关键词：vinegar backed Euphorbia ebracteolata;quality standards;content
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity of total flavonoids from Oroxylum indicum. Method: The antioxidant activity of the total flavonoids from O. indicum was determined by DPPH,salicylic acid and pyrogallol auto-oxidation. Result: Compared with Vc, the total flavonoids from O. indicum had better scavenging effect on DPPH·,·OH,O2-·in the determination of the concentration range, the EC50 was 19.73,231.70,318.93 mg·L-1 respectively; and the scavenging effect on DPPH·was better than Vc in the concentration range of 9.52-57.12 mg·L-1. Conclusion: The total flavonoids from O. indicum had better antioxidant activities,so it would be valuable to be further developed and used.关键词：Oroxylum indicum;total flavonoids;free radicals;scavenging rate;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To study a new quality standard of Xielong liquor. Method: Radix paeoniae alba,flos carthami and radix angelicae sinensis in the the formula were identified qualititively by TLC.The content of paeoniflorin, hydroxysafflor yellow A and ferulic acid was determined by HPLC with a Inertsil C8-3 column. The mobile phase was acetonitrile-0.1% phosphoric acid-tetrahydrofuran(18∶80∶2) with a flow rate at 1.0 mL·min-1.Detection wavelength was at 340 nm. Result: The TLC spots were clear and specific. The linear ranges for paeoniflorin, hydroxysafflor yellow A and ferulic acid were 23.35-373.56,5.57-89.08,2.42-38.72 mg·L-1 respectively. The average recoveries were 101.08%,99.85%,100.34%(n=6). Conclusion: The new established standard can be used for the quality control of Xielong liquor.关键词：Xielong liquor;paeoniflorin;hydroxysafflor yellow A;ferulic acid;HPLC;TLC;quality standard
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components of the volatile oil from Ershiwuwei Anshen Ganlu. Method: Water distillation was used to extract the volatile oil and the components were analyzed by GC/MS technology. Result: Thirty-two chemical components were identified. The relative amounts of 5 components were above 5%. They are cis-9-hexadecenal, bulnesol, eugenol, cedrol, guaiol. Conclusion: The experiment provides some scientific foundation for Tibetan incense in health defense field.
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of allantoin in Lithospermun zollingeri. Method: Separation and determination of allantoin were accessed by using an ODS-C18 column and a mobile phase of methanol-water at a ratio of 5∶95. The flow rate was 0.2 mL·min-1, temperature at 30 ℃ and detection wavelength at 210 nm. Result: A good linearity of allantoin was achieved in the range of 12.32-61.60 μg(r=0.999 9).The mean value of recovery was 98.1%, RSD was 1.55%. Conclusion: The method in this paper could provide an evidence for quality control of L. zollingeri and there is comparatively large difference of content of allantoin in L. zollingeri from different producing areas.关键词：Lithospermun zollingeri;HPLC;allantoin;content determination
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发布时间：2024-01-04 - 摘要：Objective: To establish a reversed-phase HPLC method for the determination of psoralen and isopsoralen in Qubaibairesi tincture. Method: The method of RP-HPLC was carried out with Scienhome kromasil ODS-1 column (4.6 mm×250 mm,5 μm); the flow rate of the mobile phase of methanol-water (55∶45) was 1.0 mL·min-1; the column temperature was maintained at 40 ℃ and the detection wavelength was set at 246 nm. Result: The linear ranges of psoralen and isopsoralen were 6.12-18.36 mg·L-1(r=0.999 0) and 6-18 mg·L-1(r=0.999 1) respectively. The recoveries were 101.57% (psoralen) and 100.39%(isopsoralen), and their RSDs were 1.6%(psoralen) and 1.5%(isopsoralen). Conclusion: The method is simple, accurate and can be used to control quality of Qubaibairesi tincture.关键词：Psoralen;Isopsoralen;Qubaibairesi tincture;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method to determine the content of chlorogenic acid in Arctium lappa,and to compare the content in A. lappa which from different regions. Method: HPLC was performed on an Agilent C18 analytical column(4.6 mm×150 mm, 5 μm)at 30 ℃ with acetonitrile-0.4％ phosphoric acid solution as the mobile phase; the detection wavelength was 372 nm and the flow rate was 1.0 mL·min-1. Result: Chlorogenic acid was separated perfectly,and its linearity was good in ranges of 0.041 8-4.18 μg. The average recovery was 100.3%. Conclusion: The validated method is simple,accurate,and had a good resoulution.It can be able to provide the basis for the quality control of A. lappa.关键词：Arctium lappa;chlorogenic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To investigate the compatible stability of danshen dripping solution and cefoxintin sodium. Method: The content of sodium danshensu, protocatechualdehyde and cefoxintin were determined after compatibility of danshen dripping solution and cefoxintin sodium within 4 hours by HPLC, and the changes of solution color, pH value and insoluble particles were observed in the simulation conditions of clinical concentration. Result: The solution color and pH value showed no obvious changes. The number of insoluble particles was increased and the content of sodium danshensu, protocatechualdehyde and cefoxintin were reduced with the extending of compatible time. Conclusion: The solution was unstable after compatibility of danshen dripping solution and cefoxintin sodium within 4 hours, therefore, not suggesting to compatibility use.关键词：danshen dripping solution;cefoxintin sodium for injection;sodium danshensu;protocatechualdehyde;cefoxintin;compatible stability
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发布时间：2024-01-04 - 摘要：Objective: To establish the methods for quality control of Yanmingxunxi Lotion (Lonicerae Japonicae Flos,Chuanxiong Rhizoma,Herba Artemisiae rupestris). Method: Lonicerae Japonicae Flos,Chuanxiong Rhizoma were identified by TLC. HPLC methods for the determination of chlorogenic acid, ferulic acid in Yanmingxunxi Lotion were established. UV method for the determination of total flavones was established. Result: The spots on the TLC plate were clear. The negative control sample didn't disturb. Chlorogenic acid showed good linearity in the range of 0.089-0.799 g·L-1, r=0.999 5 (n=5). The average recovery was 100.21%, RSD was 1.06% (n=6). Ferulic acid showed good linearity in the range of 7.434-37.17 mg·L-1, r=0.999 9 (n=5). The average recovery was 99.91%, RSD was 1.19%(n=6). Rutin showed good linearity in the range of 0.008-0.048 mg·mL-1, r=0.998 9 (n=5). The average recovery was 99.50%, RSD was 0.87% (n=6). Conclusion: The methods are simple, rapid, accurate and can be used for the quality control of Yanmingxunxi Lotion.关键词：Yanmingxunxi Lotion;quality standards;TLC;HPLC;UV
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the chloroform portion of Incarvillea arguta. Method: The chloroform portion were isolated and pured by silica gel column chromatography, HP-20 and Sephadex LH-20.Their chemical structures were elucidated by spectral data(1H-NMR,13C-NMR). Result: Five compounds were isolated from the chloroform portion.They were identified as evofolin B( 1 ),ficusal( 2 ),6-hydroxy-2-(4'-hydroxy-3',5'-dimethoxyphenyl)-3,7-dioxa-bicyclo-[3.3.0]-octane( 3 ),N-trans-feruloyl tyramine( 4 ),lysicamine( 5 ). Conclusion: All compounds were isolated from this plant for the first time.关键词：Bignoniaceae;Incarvillea arguta;the chloroform portion;chemical constituent
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of the platycodin D in Ying'er Zhike Mixture using high performance liquid chromatography-evaporative light-scattering detector (RP-HPLC-ELSD). Method: The RP-HPLC-ELSD separation was carried out on a C18 column with acetonitrile-water (25∶75) as mobile phase at a flow rate of 0.5 mL·min-1 at 30℃, and detected at 210 nm. Result: The mean recovery of platycodin D was 98.99%, and the relative standard deviation (RSD) was 0.37% (n=6). Conclusion: The method is convenient, accurate and trustworthy, and could be used for quality control of the Ying'er Zhike Mixture.关键词：Ying'er Zhike Mixture;platycodin D;RP-HPLC-ELSD
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发布时间：2024-01-04 - 摘要：Objective: Study on chemical constituents from Aucklandiae Radix. Method: The chemical constituent was isolated on chromatograph of silica gel and HPLC, their structure were elucidated on the basis of spectra data. Result: Fourteen compounds were purified and their structure were identified as caryophyllene oxide ( 1 ), 3β-acetoxy-9(11)-baccharene ( 2 ), 3-filicanone ( 3 ), chrysophanol ( 4 ), dihydro-α-ionone ( 5 ), (E)-6,10-dimethyl-9-ethylidene-undec-5-en-2-one ( 6 ), (E)-geranylactone( 7 ), dehydrocostus lactone( 8 ), costunolide ( 9 ),α-costol ( 10 ), (-)-elema-1,3-11(13)-trien-12-ol ( 11 ), (3R, 6S)-α-ionone-3-ol ( 12 ),β-ionone-4-one ( 13 ), (3R, 6R)-α-ionone-3-ol ( 14 ). Conclusion: Compound 3, 4, 12, 13, 14 were isolated from Aucklandiae Radix for the first time.关键词：Aucklandiae Radix;chemical constituents;3-filicanone;α-costol
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发布时间：2024-01-04 - 摘要：Objective: To study the dynamic accumulative law of L-asparagine in Changium smyrnioides from different collection month. Method: L-asparagine in roots of C. smyrnioides from different collection month were derived with o-phthaldialdehyde (OPA) in pre-column, and separated by RP-HPLC. L-asparagine was determined. 1 mL sample solution, 1 mL colorimetric reagent and 5 mL borax buffe (0.2 mol·L-1,pH 10.5) were settled to the volume of 10 mL and reacted 20 min; mobile phase A was 1.35% sodium acetate buffer (pH 7.3), mobile phase B was 1.35% sodium acetate (pH 7.2)-acetonitri-methanol (100∶175∶225), 0-20 min A∶B (93∶7), 20-25 min A∶B (0∶100), at the rate of 1 mL·min-1. The temperature of column was 40 ℃ and the wavelength was at 333 nm. Result: The content of L-asparagine was 0.26%-1.87% in 100-1 000 mg·L-1. Conclusion: The contents of L-asparagine was higher in May-August, January than other months.
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the essential oil from the different part of the Artemisia rupestris L. Method: Using steam distillation to extract the volatile substances from whole plant and flower of A. rupestris Using GC-MS analys the A. rupestris volatile oil. Result: Seventy-three components were identified from the whole plant volatile oil and 36 components were identified from the flower of A. rupestris. Conclusion: Two volatile oils were identified 80 kinds of ingredients. The main component of terpenes and ketones. Identified components accounted for 87.39% and 76.42% of the different parts of A. Rupestris volatile oil.关键词：Artemisia rupestris L;essential oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To investigate the target and molecular mechanism for the antitumor action of quercetin. Method: Using Autodock Vina software, dock quercetin with 91 antitumor targets; analyze the binding mode of quercetin with the targets. Result: Quercetin shows high affinity to many antitumor targets. There are 8 targets with binding free energy to quercetin lower than-9.0 kcal·mol-1. Conclusion: Quercetin exerts antitumor action by inhibiting the activity of human neutrophil collagenase(MMP-8), dihydroorotate dehydrogenase, dihydrofolate reductase, thymidylate synthase as well as several protein kinases.关键词：quercetin;antitumor;molecular reverse docking;target
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发布时间：2024-01-04 - 摘要：Objective: To compare the total flavonoids content in Epimedii folium processed by monlton suet from different growing areas and different positions of sheep or goat. Method: Twenty batches of monlton suet were collected. The processed pieces of Epimedii follum were processed by monlton suet from two species(sheep and goat), different growing region(Ningxia Province and other provinces), different positions of animals(in the abdomen or in the tail). The total flavonoids content in the processed pieces was determined by UV. Icariin was used as an external standard. The detection wavelength was set at 270 nm. Result: The linear range was 0.63-20.2 mg·L-1 for icariin, the average recovery was 100.08% and RSD was 1.50%(n=6). The largest reduction rate was from the growing region of Neimenggu Province, and the smallest one was from Hei long jiang of sheep's oil. The reduction rates of different species and positions were similar in the same region. The t test was applied to the measurement data with SPSS software. The results showed that the difference was very significant between the content of sample in Ningxia Province and in Neimenggy Province, Jiangxi Province, Heilongjiang Province (P<0.01). The results showed that the difference was significant between the content of sample in Ningxia Province and in Tianjin, Guangxi Province (P<0.05). The results showed that there was no significant difference between Ningxia Province and Shanghai, Hebei Province, Shandong Province, Henan Province, Fujian Province. Conclusion: The results showed that the content of total flovoids in processed pieces was effected by sheep's from different growth regions to a certain extent, species and positions no significant influence.关键词：monlton suet;spieces;the growth region;positions of animals;total flavonoids of Epimedii follum
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发布时间：2024-01-04 - 摘要：Objective: To discriminate ‘Genjing’ from ‘Genzhuangjing’ in the traditional Chinese medicinal materials. Method: Through collecting the bibliographic data and combined with professional practice, rhizoma Chinese medicinal materials in the 2010 edition of the China pharmacopoeia were summarized and analyzed. Result: Some Chinese medicinal materials use part of the description and Latin term were discussed and some revision suggestions were put forward. Conclusion: The standard for the ‘Genjing’ and ‘Genzhuangjing’ in the traditional Chinese medicinal materials is used to provide reference.关键词：Rhizoma;Rhizoma Chinese Medicine;Medical Position
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发布时间：2024-01-04 - 摘要：Objective: To study pharmacokinetics of bufadienolide in Shayao Chansu pill in beagle dogs. Method: Plasma metabolic fingerprint in Beagle dogs was established with UPLC/MS-TOF. The total ion chromatograms in blank plasma, plasma containing control products and plasma obtained from dogs taken Shayao Chansu pill were determined. The plasma containing bufadienolide was methodologically validated and pharmacokinetic study was carried out. Result: The UPLC/MS-TOF method for determining bufadienolide in the Beagle plasma was established, and successfully applied to the pharmacokinetic studies for Beagle plasma after administration of Shayao Chansu pill. Conclusion: The studies provided chemical evidence for safety evaluation of Shayao Chansu pill and references for clinical application of the pill.关键词：Shayao Chansu pill;bufadienolide;pharmacokinetics;Beagle dog
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Ziyin Dihaung Wan on the expression of vascular endothelial growth factor(VEGF), integrin (aVβ3) and tissue factor (TF) in rabbit choroids tissue. Method: Chorodial neovascularization (CNV) model was induced by laser, and then administrated with 9.75, 39 g·kg-1 Ziyin Dihaung Wan for 30 days and then rabbit eyeballs were performed with immunohistochemical stainingfor determination of the expression of VEGF, aVβ3 and TF in choroid. Result: Compared with the strongly positive expression of VEGF, aVβ3 and TF in model group rabbit choroidal tissue, the expression of VEGF and aVβ3 and TF was weakly positive in low, high dose Ziyin Dihaung Wan treatment groups. Ziyin Dihuang Wan at 9.75 g·kg-1 dose inhibited the expression of VEGF, aVβ3 and TF significantly (P<0.05). Ziyin Dihuangwan at 39 g·kg-1 dose inhibited the expression of VEGF, aVβ3 and TF significantly (P<0.01). Conclusion: Ziyin Dihuang Wan inhibited CNV by inhibiting the expression of VEGF, aVβ3 and TF in rabbit choroids tissue.关键词：Ziyin Dihuang Wan;CNV;VEGF;aVβ3;TF
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of berberine on adiponectin gene mRNA expression in 3T3-L1 adipocyte of insulin resistant induced by interleukin-6 (IL-6). Method: To build 3T3-L1 adipocyte model of insulin resistant induced by interleukin-6.3T3-L1 adipocytes were exposed to 20 μg·L-1 IL-6 for 48 h, the 3T3-L1 adipocytes with insulin resistant were divided into six groups randomly: normal control groups, model groups, pioglitazone (50 μmol·L-1), and low, middle, hight doses of berberine groups (10,20,50 μmol·L-1), the amount of glucose consumption was measured by detecting the glucose content in cell culture supernatants with glucose oxidase assay, the effect of berberine on glucose uptake of 3T3-L1 adipocytes was observed, and cell model of insulin resistant was identified. mRNA expression of adiponection gene was determined by real-time quantitative reverse-transcription polymerase chain reaction. Result: There was statistical difference between model group and normal control group in their amount of glucose consumption and gene expression of adiponectin, which was decreased markedly in model group (P<0.05). Pioglitazone (50 μmol·L-1), and low, middle, high doses of berberine groups (10,20,50 μmol·L-1) markedly increased both amount of glucose consumption and gene expression of adiponectin. Conclusion: Berberine can increase mRNA expression of adiponection gene and improve insulin resistant in 3T3-L1 adipocytes.关键词：berberine;insulin resistance;interleukin-6;adiponectin;3T3-L1 cells
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Zhouluotong capsule (ZLT) on diabetic peripheral neuropathy (DPN) and advanced glycation end products (AGEs) and its receptor in mice. Method: Fourty KK/Upj-Ay mice were randomly divided into model, ZLT (6.85,3.43,1.71 g·kg-1). Another ten C57BL/6 mice were as control group. Mice were given intragastrically for 12 weeks. The general appearance, fasting blood-glucose (FBG) and glycosylated hemoglobin (HbA1c) were observed. Motor nerve conduction velocity (MNCV) was observed. Pathological changes of sciatic nerve were observed by light microscope and electron microscope. The content of AGEs was examined by fluorospectrophotometry. The expression of AGEs receptor (RAGE) was examined by Real Time PCR and Western blot. Result: ZLT could ameliorate the symptom of model mice. High-dose ZLT could decrease FBG and HbA1c(P<0.05, P<0.01). MNCV were increased(P<0.05, P<0.01)and pathological lesions were alleviated in ZLT group. The content of AGE were significantly decreased(P<0.05, P<0.01). The expression of RAGE mRNA and protein were significantly decreased(P<0.05, P<0.01). Conclusion: ZLT has protective effects on DPN of mice via suppressing the expression of AGE,RAGE.关键词：Zhouluotong capsule;diabetic peripheral neuropathy;advanced glycation end products;advanced glycation end products receptor
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Acanthopanax senticosus(500,1 000 mg·kg-1) on immune function and anti-fatigue ability in sleep deprivated mice. Method: Sleep-deprivated mice model was induced by single platform technique, the immune function of sleep-deprivated mice was tested by the clearance rate of charcoal particles and the level of serum, and anti-fatigue ability was measured by the weight-loaded swimming method and the rotated performance test. Result: A. senticosus could significantly enhance the clearing index and the correction clearing index of sleep deprivated mice, the time of loaded-swimming and the residence time on the rotated sticks deprivation mice were extended significantly. Conclusion: A. senticosus can increase immune functions and anti-fatigue ability of sleep deprivated mice.关键词：Acanthopanax senticosus;sleep deprivation;immune function;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: To explore the antioxidative and antitumor effects of buckwheat flower and leaf extracts on S180 tumor-bearing mice. Method: The mice S180 tumor model was established; the model groups were divided into extraction of buckwtheal flower and leaf(EBFL)-low group (200 mg·kg-1), EBFL-high group (400 mg·kg-1), and combined with cydophosphamide(CTX,25 mg·kg-1). The solid tumor weight, ascites tumor weight and abdominal circumference growth rate and survival rate were measuredafter administration. glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) activity and malondialdehyde(MDA) content were determined. Result: After EBFL treatment for 15 d,the symptom of body weight loss was ameliorated and the growth of tumor was inhibited in tumor-bearing mice.EBFL(L,H)treatment could increase the levels of serum GSH-Px (P<0.05,P<0.01) and the activity of SOD in EBFL(H)group was obviously increased (P<0.05) compared with those of control group. The effect was similar to CTX. Compared with control group, the level of MDA was decreased significantly in tumor-bearing mice. Conclusion: EBFL can inhibit the growth of S180 mouse in a certain extent, which may be related to the increasing GSH-Px and SOD activity and decreased MDA content.关键词：extraction of buckwheat flower and leaf;antioxidative;free radical;antitumor
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Danggui Sini decoction on connective tissue growth factor(CTGF) and transforming growth factor-β(TGF-β) in skin tissue of the systemic sclerosis (SSc) mice of BALB/c. Method: BALB/c mice 60 with eight weeks old inbred strain were selected and the back fur of the central district was shaved off, and randomly divided into 5 groups (n=12 each group). The back skin of mouse in the model group and observation group was injected with 100 μL (200 mg·L-1), bleomycin (BLM) solution once each day for 3 weeks. The back skin of mouse in normal group was injected with phosphate buffered saline (PBS) 0.1 mL.After the modeling, mice in each group was given 20 mL·kg-1 by gavage daily for 3 weeks,the dosage of Danggui Sini decoction was 39, 23.4, 7.8 g·kg-1.Normal and model groups were given equal volume of saline,After the end of the experiment, the animals were killed,injected central skin was collected, andtissue homogenate was made with 2%saline and 3 000 r·min-1 centrifugation for 10 min, and then the supernatant was extracted and the content of CTGF and TGF-β were determined using ELISA method.Some of the skin was fixed in 10% formaldehyde solution, and then embedded in paraffin, and stained with hematoxylin-eosin (HE) and Masson staining, skin thickness and fibrosis index were determined, respectively, with color pathology images and Immunohistochemistry quantitative analysissystem. Result: Danggui Sini decoction could reduce the BLM induced scleroderma mouse dermal thickness (in high and middle dosage, which was 25.22±2.35,29.13±2.03 respectively,P<0.01 while in the low dosage,29.95±2.85,P＜0.05. In high-dose group and the medium dose group skin fibrosis index was reduced (86 615±8 403,103 174±18 439,respectively,P＜0.01)and skin tissue levels of CTGF (160.22±35.73),(109.51±28.64) ng·L-1,respectively,P＜0.01)and TGF-β (48.48±4.95),(63.30±7.32)ng·L-1,respectively P<0.01) were also reduced, and there was a significant dose-effect relationship. Conclusion: Danggui Sini decoction can reduce the degree of hardening of the skin in mouse model, and can reduce the the CTGF and TGF-β content.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the promoting and expelling blood circulation drugs on the expression of vascular endothelial cell growth factor (VEGF) into basic fibroblast growth factor (bFGF) in acute cerebral ischemic arterial. Method: Forty SD rats were randomly divided into sham operation group, model group, promoting blood group, expelling blood group(n=10). The acute cerebral ischemia model was established. The sham operation and model groups were given distilled water, promoting blood group was given Angelica and Chuanxiong decoction 3.6 g·kg-1, expelling group was given Sanleng Ezhu decoction 1.8 g·kg-1.Drugs were given before modeling for 7 days, after 2 days of modeling,continuous administration for 7 day was carried out of 2 h after end administration, rats were killed for collection of the basilar artery. Immunohistochemical was used for detection of basilar artery endothelial cells, VEGF and bFGF expression. Result: Compared with model group VEGF absorbance (A) of (0.348±0.086), in promoting blood group (0.443±0.061) and expelling blood group (0.558±0.12), VEGF protein expression was increased in a statistically significant difference (P<0.01 to P<0.05). Compared with promoting blood group, the effect of expelling blood group was stronger, the difference was statistically significant (P<0.05). Compared with the model group A of bFGF (0.394±0.080), in promoting blood group bFGF (0.553± 0.097) protein expression was elevated, the difference was statistically significant (P<0.01), in the expelling blood group bFGF (0.302±0.084) protein expression decreased, the differences statistically significant (P<0.05). Conclusion: Promoting blood circulation drugs and expelling blood circulation drugs can promote the expression of VEGF protein, and expelling the drug showed more potent; promoting blood circulation drugs increased the expression of bFGF protein, expelling blood circulation drugs were able to reduce the expression of bFGF protein. This shows that for the mechanism of promoting blood circulation,promoting and expelling blood circulationdrugs have both similarities and differences.关键词：promoting blood circulation drugs;expelling blood circulation drugs;acute cerebral ischemia;VEGF;bFGF
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects and mechanism of Xinglou Chengqi decocotion (XLCQD)and Buyang Huanwu decoction (BYHWD) on Fas/Fasl and Caspase-3 of regulating genes of neurons apoptosis caused by cerebral ischemia. Method: Rats were randomly divided into sham, model, Nimodipine, BYHWD and XLCQD groups. Focal cerebral ischemia models were established by middle cerebral artery occlusion with nylon thread. Rats were given with XLCQD(5.0 g·kg-1),BYHWD(13.0 g·kg-1)and nimodipine(10.8 mg·kg-1)by ig before the model preparation,once a day after the operation. At 1, 3, 7 d after operation, neurons apoptosis, expressions of Fas, Fasl and Caspase-3 were determined using the method of immunohistochemistry. Result: A few expressions of Fas(16.60±1.36), Fasl (19.40±1.72)and Caspase-3 (16.35±1.63)could be observed in rat ’s brain of sham group. In each model group, expressions of Fas(45.83±1.44,36.25±1.60,31.37±2.27), Fasl(44.27±2.25,37.68±2.01,34.15±1.55), Caspase-3(37.18±2.78,29.50±2.07,25.26±3.04)all increased(P<0.01). Fas and Fasl expression in each administrated group all decreased, and the expression of Caspase-3 in Nimodipine 1 d group, each XLCQD and BYHWD group was higher. Fas and Fasl expressions in each XLCQD group and 7 d BYHWD group and Caspase-3 in 3 d XLCQD group all decreased than that in Nimodipine groups. The expressions of Fas in 1 d and 3 d, Fasl in 1d, Caspase-3 in 3 d XLCQT groups all decreased than that in BYHWD groups. Conclusion: It was shown that Fas/Fasl up-regulation of apoptosis could be caused by cerebral ischemia,and XLCQD and BYHWD could all inhibit the level of Fas, Fasl and Caspase-3.The role of XLCQD was more earlier and significant in down-regulating the expressions of Fas, Fasl and Caspase-3, especially at 1d after cerebral ischemia.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Tongxinluo (TXL) on hypertrophy of H9c2 cardiomyocytes and connexin 43 mRNA expression induced by isoproterenol (Iso). Method: Cultured H9c2 cardiomyocytes were randomly divided into four groups: control group, TXL group, Iso group, and Iso+TXL group. After 72 hours of culture, cell size was determined by phase contrast microscope, the protein concentrations were measured by BCA method and the expression of Cx43 mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR). Result: Cell size of H9c2 cardiomyocytes of the Iso group (P<0.05) was increased more prominently than the control group. Compared with Iso group, Tongxinluo attenuated the increase of cardiomyocytes size (P<0.05). H9c2 cardiomyocytes protein concentrations in the Iso group (P<0.05) was increased more significantly than the control group. Although Tongxinluo didn't affect the normal H9c2 cardiomyocytes protein concentration, it inhibited the increase of protein concentrations induced by Iso (P<0.05).Iso decreased Cx43 mRNA expression of H9c2 cardiomyocytes more evidently than that of the control group (P<0.05), which was inhibited by Tongxinluo (P<0.05). Conclusion: Tongxinluo can prevent the hypertrophy of H9c2 cardiomyocytes and down-regulate of Cx43 mRNA expression induced by Iso.关键词：Tongxinluo;cardiac hypertrophy;H9c2 cardiomyocytes;connexin 43;isoproterenol
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the dynamic changing of troponin(cTn-T) and observe the treatment effect of Guanxin Suhe capsule on acute myocardial infarction(AMI) in rats and investigate its mechanism. Method: Rats were selected to establish acute myocardial infarction model. The rats were divided randomly into five groups and treated with pure water,high-dose Guanxin Suhe (0.8 g·kg-1),low-dose Guanxin Suhe (0.4 g·kg-1),captopril respectively for 5 days, then AMI model were established. cTn-T,aspartate aminotransferase(AST),creatine kinase enzyme(CK), MBisoenzyme of creatine kinase(CK-MB) was determined after ligating 31 h. Result: The area of myocardial infarction in the Guanxin Suhe capsule high dosage group was reduced, cTn-T and AST decreased, showing a statistical significance compared with the model group (P<0.05, P<0.01,P<0.01).The effect of high-dose and low-dose Guanxin Suhe capsule on ECG in acute myocardial infarction rats was observed, the heights of ST segment were reduced just after ligating 0 h and 31 h. Conclusion: Guanxin Suhe capsule has significant effect on myocardial infarction, it treats myocardial infarction through regulating cTn-T and AST.关键词：troponin;myocardial enzyme;Guanxin Suhe capsule;myocardial infarction
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发布时间：2024-01-04 - 摘要：Objective: To explore the effects of total saponin of Dioscoreae Nipponicae Rhizoma(RDN) on in vitro apoptosis of rIL-1β induced fibroblast-like synoviocytes(FLS), and further to investigate the PI3K/AKT signal mechanism. Method: FLS were primarily cultured. Model group: 10 μg·L-1 rIL-1β, RDN group: 10 μg·L-1 rIL-1β and 100 μg·L-1 RDN. Western blot method was applied to examine the expression of p-PI3K protein and p-AKT protein degradation in rIL-1β induced FLS stimulated with total saponin of RDN. Result: Compared to control group, expression of p-PI3K and p-AKT protein increased in model group, respectively 162.66±2.34 and 157.03±1.84(P＜0.05), total saponin of RDN inhibited rIL-1β induced phosphorylation of AKT and PI3K in FLS, respectively 67.84±1.24 and 71.66±1.20 (P＜0.01). Conclusion: Total saponin of RDN can inhibit FLS PI3K/AKT signal pathway. Total saponin of RDN may provide a new therapeutic approach in treatment of gouty arthritis.关键词：total saponin of RDN;rIL-1β;FLS;gouty arthritis
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发布时间：2024-01-04 - 摘要：Objective: Study the effects and mechanism of Banxia Xiexin decoction on gastric cancer. Method: Banxia Xiexin decoction was divided into Pungent openning(dried ginger,pinellia ternate)group,Bitter Descending(coptis chinensis,scutellaria) group and Invigoration(glycyrrhiza,ginseng) group by its different nature and flavor. 1×104cells/wells were planted at 96 wells plate, drug concentrations were 4, 2, 0.5, 0.125 g·L-1, cell proliferation was detected by MTT method. 1×105 cells/wells were planted in 6 wells plate. The high and low concentration of whole Party and Bitter Descending drugs was respectively 0.5, 0.125 g·L-1. The high and low concentration of Pungent openning and invigoration was respectively 4,2 g·L-1. Effect of Banxia Xiexin decoction and compositions on gastric cancer SGC-7901 cells proliferation, cell cycle and apoptosis ratio were detected and compared with known positive drug 5-fluorouracil (5-FU). Result: Banxia Xiexin decoction group, Bitter Descending group and Pungent opening group could inhibit the proliferation of SGC-7901 cell, the IC50 values of its were 1.645, 1.446, 3.867 g·L-1 respectively, and increased SGC-7901 cells in S phase and G0-G1 phase percentage with promoting apoptosis in dose-dependent manner, but invigoration group had no such effect. Conclusion: Banxia Xiexin decoction and its compositions can inhibit the proliferation of SGC-7901 cell, Bitter Descending is strongest and Invigoration group is the weakest,which may be related to blocking of cells to G1/S phase and inducing cell apoptosis关键词：Banxia Xiexin Decoction;SGC-7901 cells;decomposition study;cell cycle;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Xiaochaihu Decoction on damage of gut-liver-brain in CCl4/ethanol-induced mouse hepatocellular carcinoma. Method: Hepatocellular carcinoma group(HCC) were induced by subcutaneous injection with 25% CCl4 olive oil solution(5 mL·kg-1 twice per week) and allowed free access to a 8% ethanol solution as drinking fluid for 4 weeks,and allowed free access to 0.5% CCl4-8% ethanol solution as drinking fluid for 20 weeks; in HCC combined with liver depression and spleen deficiency(LDSD) group(HCC-LDSD group), on the basis of HCC modelling, the mice were stimulated with the factor of LDSD,squeezing tails(30 min·d-1),solitary breeding and intermittent fasting for 4 weeks, solitary breeding and intermittent fasting for 20 weeks; Xiaochaihu decoction(XCHD) treated group was administered by gavage for 8 weeks after 4 weeks of the HCC-LDSD modelling. The mortality rates and HCC incidence rates were calculated, weight and clinical signs were monitored daily. The degree of tissue injuries in the gut and liver were studied using a scoring system, and brain weights were measured. Result: The mortality rate in HCC-LDSD group was higher than that in the HCC group, after treatment with XCHD, the mortality rate decreased significantly.In death mouse of HCC group,significantly more injuries in small intestine,cecum,liver and fecal loading in the cecum, with the increased in brain weights,the most in HCC-LDSD group, after treatment with XCHD, all of which were improved. HCC incidence rate in the HCC-LDSD group was higher than that in the HCC group, after treatment with XCHD,the HCC incidence rate decreased significantly.In survival mouse of HCC group,significantly more injuries in small intestine,cecum, liver(mainly hyperplasia), with slight decreased in brain weights,the most in HCC-LDSD group, after treatment with XCHD, all of which were improved. Correlative analysis showed that there was a positive or negative correlation between small intestine injuries,cecum injuries,fecal loading in the cecum,liver injuries and brain weights in death mouse and survival mouse. Conclusion: XCHD offered protection against CCl4/ethanol-induced inflammation by decreased the injury of gut-liver-brain, and improved gut-liver-brain interactions, and ultimately decreased mortality rates and HCC incidence rates in CCl4/ethanol-induced mouse HCC,it need further research to understand the molecular mechanism of effects of XCHD.关键词：Xiaochaihu decoction(XCHD);ethanol;CCl4;liver depression and spleen deficiency(LDSD);tissue injury;gut-liver-brain interactions
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发布时间：2024-01-04 - 摘要：Objective: It was reported that Sipunculus nudus polysaccharide(SNP) had effects on fatigue in the early time in our laboratory, in the article we studied the anti-fatigue effects of purified SNP on the fatigue resistance. Method: The treatment groups were given various doses of SNP (50, 100, 200 mg·kg-1) for 7 consecutive days, respectively, and the longest swimming time was detected after loaded swimming test. the level of serum urea nitrogen (BUN), muscle glycogen and the activity of hepatic antioxidase including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected. Result: After 7 days, the mouse swimming time in the blank control group was(115±40)s, and the swimming time with medium and high dose feeding of purified Sipunculus nudus polysaccharide was(206±93),(203±74)s, respectively, longer than blank control groups(P<0.01). The serum urea nitrogen of the treated groups was(8.72±0.46)mmol·L-1, and that in medium and high dose group was(7.55±0.70),(7.89±0.42)mmol·L-1, respectively, lower than blank control groups(P<0.01). Besides, the muscle glycogen in the blank control group was(0.41±0.04)mg·g-1, and that in medium and high dose groups was(1.01±0.18),(1.06±0.22)mg·g-1, higher than blank control groups(P<0.01).Moreover, the activity of SOD and GSH-Px in high dose group in hepatic tissue was increased compared with blank control group(P<0.01). Conclusion: SNP has noticeable anti-fatigue effect on mice, especially in intermediate at dose group (100 mg·kg-1) and its strongest effect on most biomarkers is found when given an intermediate dose.关键词：Sipunculus nudus polysaccharide;loaded swimming test;muscle glycogen;serum urea nitrogen;hepatic antioxidase;anti-fatigue
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发布时间：2024-01-04 -
Influence of the Extract of Polygoni Multiflori Radix on Follicle Growth and Hair Growth of C57BL/6J
摘要：Objective: To study the influence of the extract of Polygoni Multiflori Radix on follicle growth and hair growth of C57BL/6J. Method: The model of organ culture of mouse vibrissa follicle in vitro was used and the time and velocity of hair growth were recorded to investigate the influence of follicle growth. The experiment had 9 groups: blank control group,positive control group (Zhangguang 101), different content of the extent for 75,150,300,600,1200,2 500,5 000 μg·L-1,24 shadow mask, 2 folliculus pili per hole and cultivating 12 d. Treated in vitro on hairless mice skin was used to investigate the effects of promoting hair growth with different dose of Polygoni Multiflori Radix. This experiment had 5 groups: positive control group (Zhangguang 101), blank control group,the extract of Polygoni Multiflori Radix high dose group(2 g·mL-1),medium dose group (1 g·mL-1),low dose group (0.5 g·mL-1). Result: The experiment of organ culture of mouse vibrissa follicle in vitro showed that the extract of Polygoni Multiflori Radix could accelerate follicle growth in the early, intermediate and terminal phases and prolong the hair growth time with the dose of 300,600,1 200 μg·L-1 in a dose-dependent manner and there were significant differences in comparison with normal group. When the content was 300 μg·L-1,the velocity of hair growth were(0.236±0.061),(0.427±0.078),(0.325±0.054)mm·d-1 on the second, sixth and twelve day, the time of hair growth was (9.60±1.43)d, the differences were significant between the test groups with the blank control and positive control group(P<0.01). When the content was 600 μg·L-1,the velocity of hair growth were(0.313±0.044),(0.522±0.084) mm·d-1 on the second and sixth day, the differences were significant between the test groups with the blank control group(P<0.01)and between the test groups with the positive control group (P<0.05), the velocity of hair growth was(0.439±0.064)mm·d-1 on the twelve day, the time of hair growth was (11.00±1.15)d, the differences were significant between the test groups with the blank control group (P<0.01). When the content was 1 200 μg·L-1,the velocity of hair growth were (0.406±0.053), (0.642±0.067), (0.475±0.036)mm·d-1 on the second, sixth and twelve day, the time of hair growth was (12.40±1.43) d, the differences were significant between the test groups with the blank control group(P<0.01).The experiment of pharmacodynamics in vivo showed the extract of Polygoni Multiflori Radix can make hair grow from anestrum to growing period with significant difference. High dose group can accelerate the production of melanin in skin than middle and low dose group. The time of high dose group were (9.06±0.43) d when the skin became pale from pink, (14.68±1.19) d when hair came out and (26.86±2.02) d when hair was overgrown, the differences were significant between the test groups with the blank control group(P<0.01). In the later period of hair growth, the dependence on its concentration is not significant. Conclusion: The extract of Polygoni Multiflori Radix can lead hair growth of C57BL/6J mice from anestrum to growing period, the result of experimental in vivo and in vitro is coincident.关键词：Polygoni Multiflori Radix;C57BL/6J mice;growing phase of hair cycle;follicle growth- 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate the serum amino acid variation in diabetic mice. Method: SPF mice were fed with high-fat diet for 4 weeks and then injected with streptozotocin(STZ). Mice were successively administrated with ethyl acetate extract Coreopsis tinctoria(8 g·kg-1·d-1) for 30 days. HPLC was used for the analysis of amino acid metabolites in serum samples. These data were analyzed by partial least square-discrimiant analysis (PLS-DA). Result: Compared with the control mice,the metabolism profiles of amino acid in the model mice were significantly different from that of the control mice. Pattern recognition method was applied and 4 amino acid markers were different between all the groups. Conclusion: The amino acid metabolism profiling may reflect to certain extent the pathological changes of type 2 diabetic mice. This work can provide basis for further researches on the interpretation of drug property of evidence.关键词：Xinjiang Coreopsis tinctoria;type 2 diabetes;metabonomics study;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of Naili capsule on60Co γ-ray induced injury and provide an experimental basis for the development of Naili capsule. Method: Male KM mice were divided into model group, the positive drug group (dose 0.98 g·kg-1) and Naili capsule low, medium and high doses (for 1.82, 3.64, 7.28 g·kg-1) group, ig, for 7 days consecutively. Experiment was divided into two parts: mice were radiated by 8.5 Gy of 60Co γ ray radiation, survival rate of 30 days was observed and the average survival time of mice was calculated; mice were radiated by 6.5 Gy60Co γ ray radiation. Peripheral blood cells, bone marrow nucleated cell count, the role of the index of immune organs, bone marrow histopathology of mice were detected on 7 days after radiation. Result: The 30-day survival rate (improved survival was 37.5%) and the mean survival time(24.88 d) of mice administered Naili capsule (for 3.64 g·kg-1) were all much higher than those of the model group and protection factor was 1.30.White blood cells of mice irradiated were improved (1.34×109/L on 3 days after radiation and 0.89×109/L on 7 days after radiation) and the marrow nucleated cell counts could be increased (5.89×106 in every femur). The damage of bone marrow hematopoietic cells in mice could be reduced. Conclusion: Naili capsule has significant radioprotective effect in mice.关键词：Naili capsule;radiation injury;radioprotective effect
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Jiangzhi Ⅱ Preparation on experimental hyperlipemia and atherogensis in rabbits. Method: The hyperlipemia and atherosclerosis model in rabbits was established by feeding high-fat diet. All animals were randomly divided into normal group,model group,simvastatin group,Jiangzhi Ⅱ high,middle and low dose groups (4.6,2.3,1.2 g·kg-1).After eight weeks of treatment,the content of total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL), high density lipoprotein(HDL),nitric oxide(NO) and endothelin (ET-1) in serum were determined. The extent of aortic atherosclerosis was observed and the pathological examination was done. Result: In the Jiangzhi Ⅱ treatment groups, the concentration of TC,TG,LDL,ET-1 in serum was decreased markedly,the concentration of HDL and NO was increased remarkably,the extent of aortic atherosclerosis and the atherosclerotic lesion were lightened obviously. Conclusion: Jiangzhi Ⅱ Preparation can effectively decrease the serum lipids and prevent the formation of atherosclerosis.关键词：hyperlipemia;the extent of aortic atherosclerosis;rabbits;triglyceride;high density lipoprotein
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发布时间：2024-01-04 - 摘要：Objective: Using genetically modified Kkay type 2 diabetic mice as the model, to observe the effect of Xinkaikujiang traditional decoction (XKKJ-group 1) and Xinkaikujiang Formula granules (XKKJ-group 2) for insulin sensitivity and blood lipids in KKay type 2 diabetic mice. Method: According to the blood sugar, thirty-six male KKay mice (10 weeks old) were randomly divided into 4 groups, including the model group, the rosiglitazone group (0.67 g·kg-1·d-1), the XKKJ-group 1 and the XKKJ-group 2 (8.7 g·kg-1·d-1). The control group was consisted of 9 C57BL/6J male mice(10 weeks old). The treatment groups were administered orally once a day, and both the control group and model group were given 0.5% sodium carboxymethylcellulose (CMC) solution. After 4-week treatment, the random blood sugar, fasting blood sugar (FBG), serum insulin and blood lipids were determined, and the insulin sensitivity index was calculated. Result: After 4-week treatment, compared with the control group, the model group's ISI was significantly lowered(P<0.01), FBG, triglyceride (TG) and total cholesterol (TC) were increased significantly(P<0.01). Compared with the model group, ISI in XKKJ-group 1 and XKKJ-group 2 was significantly improved (P<0.01), FBG significantly lowered(P<0.01)TG and TC lowed. There were no difference between XKKJ-group 1 and XKKJ-group 2. Conclusion: The Chinese medicine of XKKJ could improve the insulin resistance in KKay type 2 diabetes mice, increase insulin sensitivity and rnduce the blood sugar level, lipid levels. The efficacy is not related to the dosage form.关键词：Xinkaikujiang formula;KKay type 2 diabetes mice;insulin sensitivity;blood glucose;blood lipid.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the hypoglycemic effect of polysaccharide from bovine heart persimmon leaf in diabetic mice model induced by streptozotocin. Method: Diabetic mice model was established by injection of streptozotocin, then the mice were randomly divided into 5 groups: model group, dimethyldiguanide group (0.32 g·kg-1), low-, medium-and high-dosage groups of polysaccharide of bovine heart persimmon leaf (0.3, 0.6, 1.2 g·kg-1). The drugs were given to mice daily for 15 consecutive days. Fasting was carried out before administration for 12 h at the end of 15 days. The blood sample was collected via evulseing the mice eyes, and centrifuged to obtain serum for test. Glucose oxidase (GOD) was used to examine the index of glycemia for fasting plasma glucose(FBG); the levels of free fatty acids(FFA) and glutathione(GSH) in serum as well as the contents of total superoxide dismutase(T-SOD)and malondialdehyde(MDA) in liver tissue were tested by biochemical method. The expressions of IRS-1, INSR in pancreas were measured using Western blot analysis. Result: Compared to model control group, the polysaccharide could significantly decrease the level of blood-sugar in diabetic mice, the difference was statistically significant (P<0.05), and increase GSH level in serum, while reduce the NEFA level, difference was statistically significant (P<0.05). Compared with the model, the polysaccharide could also elevate T-SOD level in liver tissue, while reduce the content of MDA, difference was statistically significant (P<0.05). It also up-regulated the levels of INSR, IRS-1 protein in pancreatic tissue, difference was significant (P<0.05). Conclusion: The polysaccharide of bovine heart persimmon leaf can play the role of hypoglycemic activity though the mechanism may be related to the protective effect of lipid peroxidation injury in liver tissue and to improve INS signal transduction pathway in the pancreatic tissue of diabetic mice.关键词：polysaccharide from bovine heart persimmon leaf;diabetes;hypoglycemic activity;oxidation resistance;signaling pathway
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发布时间：2024-01-04 - 摘要：Objective: To observe the inhibitory effect of Changweiqing on liver metastasis of colorectal cancer in mice, and investigate the related mechanism of Changweiqing on liver metastases of colorectal cancer, by Chemokine Ligand 12 (CXCL12)/Chemokine Receptor 4 (CXCR4)the signal transduction pathway and the expression of Matrix metalloproteinase 9(MMP9). Method: We established the liver metastases model of mice with implanted colon cancer CT26.Mice were divided into four groups, namely sham operation group, model group, the low dose group and the high dose group of Changweiqing. Sham operation group and model group were given normal saline, Changweiqing low and high dose groups were administrated with 10.37,20.74 g·kg-1 respectively. The HE staining was performed to judge liver metastasis. The expression of the CXCR4, CXCL12, and MMP9 was detected by immunohistochemistry and quantitative real-time PCR in the colorectal tumor tissues and the normal colon tissue. Result: The weight inhibition rates of colon in situ tumor in Changweiqing low and high dose groups were 24.73%, 45.91%; while the volume inhibition rates were 27.93%, 63.48%. The liver metastasis rates were 75%, 37.5%, 12.5% in the model group, Changweiqing low and high dose groups respectively. The CXCR4, CXCL12, MMP9 protein and mRNA expression of the colon tumor tissue in model group were significantly higher than that in the sham operation group (P<0.05), while the low and high dose Changweiqing could reduce their expressions (P<0.05). CXCR4 protein and mRNA expression in Changweiqing low dose and high dose groups showed significant decrease compared with those in model group (P<0.05). CXCL12, protein expression in Changweiqing low dose and high dose groups was significantly decreased compared with that in the model group (P<0.05). CXCL12 mRNA in Changweiqng high dose group was significantly decreased compared with that in the model group (P<0.05). For MMP9 protein and mRNA expression, Changweiqing high dose could decrease them significantly compared with those in the model group (P<0.05). Conclusion: Changweiqing inhibits the colorectal cancer tumor growth in situ and the incidence of liver metastasis in mice, The mechanism is likely related to the down-regulation of CXCR4, CXCL12, MMP9 expression in the tumor tissue.关键词：Changweiqing;colorectal cancer;liver metastasis;CXCL12;CXCR4;MMP9
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发布时间：2024-01-04 - 摘要：Objective: To study the antiphlogistic and antibacterial effect of aqueous extract from Mussaenda pubescens. Method: Mice were divided into 5 groups randomly:normal saline (NS)group,positive group,high dose group,middle dose group and low dose group of aqueous extract from M. pubescens (33.6,16.8,8.4 g·kg-1). After 4 d by ig administration, dimethybenzene-induced mouse ear edema model was used to evaluate the anti-inflammation effect. Rats were divided into 5 groups randomly:normal saline (NS) group,positive group,high dose group,middle dose group and low dose group of aqueous extract from M.pubescens (24,12,6 g·kg-1). Using models of carrageenan-induced paw edema and cotton-peller-induced granuloma, anti-inflammation effect was evaluated. Bacteriostasis effect of aqueous extract from M.pubescens was investigated by the method of bacteriostatic test in vitro. Result: The middle and high dose of the extract could significantly relieve ear-swelling caused by dimethylbenzene (P<0.05).The middle and high dose of the extract could significantly inhibit paw swelling in rats within 2, 3 hours (P<0.05).The granuloma quality was significantly reduced by the extract compared with the control group (P<0.05). The aqueous extract had antibacterial effects on Staphylococcus aureus, Escherichia coli, Pneumococcus, Streptococcus, Shigella,and the minimum inhibitory concentration(MIC) was 125, 31.3, 15.7, 62.5, 62.5 mg·kg-1,respectively. Conclusion: Aqueous extract from M. pubescens has significant anti-inflammatory and antibacterial effects.关键词：Mussaenda pubescens;anti-inflammatory;antibacterial;ear edema;paw edema;granuloma
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the mice spleen lymphocyte proliferation and transformation effect of Xiangfu Siwu decoction(XFSWD) and explore the bioactive constituents. Method: The samples were prepared by macroporous adsorptive resins. mice spleen lymphocyte proliferation and transformation models were adopted to evaluate the immune related activities of XFSWD and its different separated fractions in vitro. The chemical constituents in the most active fraction were identified and determined by HPLC. Result: By mice spleen lymphocyte proliferation and transformation experiments, XFSWD, XFSW-3 and XFSW-7 showed high effect. Four main compounds including albiflorin, peoniflorin, ferulaic acid, senkyunolide I in active fraction XFSW-7 were identified and quantified by HPLC-DAD. The contents were 5.973%, 5.902%, 1.374% and 0.014%, respectively. Conclusion: Albiflorin, peoniflorin, ferulaic acid, senkyunolide I were the potential active constituents. XFSWD had the common material basis.关键词：Xiangfu Siwu Decoction;mice spleen lymphocyte;bioactive constituents
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Junzi Fuzheng decoction in enhancing anti-tumor efficacy of cyclophosphamide and reducing its toxicity. Method: Fifty MFC bearing mice models were established and then divided randomly into five groups: control group, cyclophosphamide(CTX) group(30 mg·kg-1), Junzi Fuzheng decoction (high,medium and low dose,20,10,5 g·kg-1) combined with CTX(30 mg·kg-1)groups.There were 10 mice in each group. After 10 days of treatment, all mice were killed and the tumor inhibiting rates and the index of immunological function were observed. Result: The tumor inhibition rate was higher in each Junzi Fuzheng decoction+CTX group than that in control group (P <0.01).The tumor inhibition rate of high-dose and medium-dose Junzi Fuzheng decoction+CTX group was higher than that in CTX group (P <0.01,P <0.05).The indexes of spleen and thymus as well as proliferation of T lymphocytes and nature killer cell activity in CTX group were lower than those in the high-dose Junzi Fuzheng decoction+CTX group (P <0.01,P <0.05).The counting of peripheral white blood cells and bone marrow nucleated cells in CTX group was lower than those in the high-dose and the medium-dose Junzi Fuzheng decoction+CTX groups (P <0.01,P ＜0.05). Conclusion: Junzi Fuzheng decoction can enhance the efficacy of chemotherapy and reduce its toxicity in the MFC-burdened mice exposed to CTX.关键词：Junzi Fuzheng decoction;cyclophosphamide;enhancing effect and reducing toxicity;tumor chemotherapy;tumor inhibiting rate
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发布时间：2024-01-04 - 摘要：Objective: To observe the joint effect of shear stress and Shenlian (SL) extracts on E-selectin and neuclear factor κB(NF-κB) expression in endothelial cells. Method: 2×2 factorial design was employed to analyze the interactions between shear stress and SL extracts. Bioflux1000 microfluidic device was used to control two levels of shear stress. E-selectin and NF-κB expression stimulated by tumor neerosis factor -α(TNF-α) in human umbilical vein endothelial cells(HUVECs) were detected by using immunofluorescence method after pretreatment 10 hours with different level,of shear stress and SL extract. Result: There was an interaction associated with each factor in regulating E-selectin expression. Combined treatment of SL extract with 10 dyn·cm-2 shear stress led to a synergistic decrease in E-selectin expression in endothelial cells. As a single factor, SL extract or shear stress alone could decrease NF-κB expression significantly. Conclusion: Shear stress may influence the SL extract's atheroprotective effect through downregulation of E-selectin via NF-κB in endothelial cells.
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发布时间：2024-01-04 - 摘要：Objective: To study the analgesic and anti-inflammatory actions of Yuanshengtong formula (YSTF), as well as the acute toxicity of YSTF. Method: The median lethal dose (LD50) of YSTF to mice was calculated with Bliss method. Writhing method and hot plate method in mice and tenderness test in rats were used to evaluate the analgesic effect. Paw swelling caused by carrageenan in rats, increased permeability of abdominal cavity induced by acetic acid in mice, adjuvant arthritis model in rats was established by Freund's complete adjuvant. The anti-inflammatory effect of YSTF was evaluated. Result: The LD50 of YSTF was 30.10 g·kg-1 with the 95% confidence range of 25.85-34.66 g·kg-1. YSTF had prominent analgesic and anti-inflammatory effects both in mice at 0.184 and 0.369 g·kg-1 and more than 0.184 g·kg-1 in rats. Conclusion: YSTF has significant analgesic effect and exhibits low toxicity. It can be applied to slow down rheumatic arthritis and other disease with sharp pain.关键词：Yuanshengtong formula;analgesic effect;anti-inflammatory effect
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Guizhi Fuling pills (GFP) on pain and matrix metalloproteinases expression in rats with endometriosis. Method: Rat model of endometriosis (EMS) was established. The model rats were divided into the model group, ibuprofen group (0.054 g·kg-1), danazol group (0.036 g·kg-1), and GFP low, high dose groups (0.256, 1.024 g·kg-1). The treatment in each group was lasted for 4 weeks. Plasma 6-ketone prostaglandin F1α (6-keto-PGF1α), beta endorphins (beta EP) and thromboxane B2 (TXB2) were determined by radioimmunoassay. Tissue of uterus endometriosis was obtained, and lesions and size were evaluated. The expression of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9) in ectopic endometrium was determined. Result: GFP could reduce the volume of ectopic endometrium significantly (P<0.01). Two doses of GFP could increase plasma 6-keto-PGF1α, beta EP level, and reduce TXB2 level (P<0.01). Two doses of GFP could reduce the expression of MMP-2 and MMP-9 (P<0.05, P<0.01). Conclusion: GFP inhibits the growth of ectopic endometrium. The mechanism may be related to inhibition of the expression of MMP-2 and MMP-9.The pain relieving of GFP may be obtained through the balance prostaglandin level, alleviation of spasms of smooth muscle, and elevation of endorphins.关键词：Guizhi Fuling pills;endometriosis;6-ketone prostaglandin F1α;beta endorphins;thromboxane B2;matrix metalloproteinases-2;matrix metalloproteinases-9
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect and mechanism of Linggui Zhugan decoction on cardiomyocyte apoptosis caused by ischemia reperfusion in rats. Method: Forty SD rats were randomly divided into sham, model, simvastatin and Linggui Zhugan decotion group. Rats insimvastatin group were perfused with Simvastatin 20 mg·kg-1·d-1, rats in Linggui Zhugan decotion group were perfused with Linggui Zhugan decotion 50 g·kg-1·d-1, other two groups were given NS 1.0 mL·kg-1 a week before the operation. Myocardial ischemia reperfusion injury(MIRI) model was established by ligating the left descend coronary artery. After ischemia 30 min and reperfusing 2 hours, the changes in cardiomyocyte apoptosis were detected by flow cytometry, the expression of Smad3, Smad 7 protein were detected by immunohistochemical staining in cardiomyocyte. Result: Apoptosis rate and the level of Smad3 protein in model group were higher significantly than those of sham group (P＜0.01),but the level of Smad7 protein was lower than that of sham group(P＜0.01). Apoptosis rate and the level of Smad3 protein in Linggui Zhugan decoction and Simvastatin group was lower significantly than those of model group(P＜0.01),but the level of Smad7 protein was higher than that of model group(P＜0.01). Conclusion: Linggui Zhugan decoction can inhibit cardiomyocyte apoptosis in rats with MIRI, which is realized by up-regulating Smad7 protein expression and down-regulating Smad3 protein expression.关键词：Linggui Zhugan decoction;myocardial ischemia reperfusion injury;apoptosis;Smad protein
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Tongfeng Shuning tablet (TST) on hyperuricemia in mice. Method: TST (1.1,2.2,4.4 g·kg-1) and allopurinol tablet (40 mg·kg-1) were given ig.for 7 d in mice. Hyperuricemia model in mice was established by uric acid (UA),and UA in serum was determined. TST (0.75,1.50,3.00 g·kg-1) and allopurinol tablet (20 mg·kg-1) were given ig.for 7 d in rats, meanwhile, hyperuricemia in rats was established by adenine and ethambutol. UA, blood urea nitrogen (BUN) and creatinine (Cre) in serum were determined, kidney weight index and histopathological changes were also observed. Result: Compared with the mice model UA[(310.50±74.88) μmol·L-1], UA levels in middle and high doses of TST were [(124.00±57.33), (56.27±42.24) μmol·L-1](P<0.05,P<0.01). The UA level in high dose of TST was 86.10±32.09 μmol·L-1 lower than that (137.70±43.21 μmol·L-1)of the rat model (P<0.05). The level of Cre in middle and high dose of TST were [(45.80±8.46), (48.50±6.80) μmol·L-1], lower than that [(59.60±13.53) μmol·L-1] in the rat model(P<0.05,P<0.05). All doses of TST could decrease the kidney weight index(P<0.05,P<0.05,P<0.05) and relieve kidney histopathological damage(P<0.05,P<0.01,P<0.01).Conclusion: TST had significant effect on hyperuricemia.关键词：Tongfeng Shuning tablet;uric acid;hyperuricemia
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Asparagus instant powder (AIP) on sleep in mice. Method: Healthy KM male mice with the body weight of 18-22 g were divided into groups randomly. Then the mice were gavaged with AIP (containing 15% saponins) of low, middle and high doses (85,170,510 mg·kg-1); control group (water)and positive control (Jujuboside 360 mg·kg-1) respectively. The treatment was lasted for 30 days, the sleep-producing test was carried out by sub-threshold dose of sodium pentobarbital, and the incubation period test with sodium pentobarbital and the sleep extending test with sodium pentobarbital were also conducted. Result: AIP showed good sleep-producing action in sub-threshold dose test of sodium pentobarbital, could significantly shorten the incubation period induced by sodium pentobarbital, and could significantly prolong sleeping time induced by sodium pentobarbital. Conclusion: AIP can improve the sleep quality.关键词：Asparagus instant powder;sleep;asparagus saponins
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发布时间：2024-01-04 -
Study on the Antidepressant-like Effect of Bupleuri Radix and Paeoniae Radix Alba in Different Doses
摘要：Objective: To study the antidepressant-like effect of the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba, single use of Bupleuri Radix and single use of Paeoniae Radix Alba in different doses. Method: Male ICR mice were divided into 11 groups: control group, fluoxetine (0.015 g·kg-1) group, the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba (1∶1) in high, middle and low doses (32, 16, 8 g·kg-1) groups, single use of Bupleuri Radix in high, middle and low doses (32, 16, 8 g·kg-1), single use of Paeoniae Radix Alba in high, middle and low doses (32, 16, 8 g·kg-1) groups. The open field test (OFT) was used to exclude false-positive results at 7 d after the administration (ig).And the tail suspension test (TST) and the forced swimming test (FST) were used to study the antidepressant-like effect of different groups. Result: There was no significant difference in different groups in the experiment of OFT. Different groups reduced the immobility time both in the TST and FST. Most obviously in the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba(1∶1) in high doses (32 g·kg-1), in the TST, the immobility time was (51.3 ± 31.7) s, and in the FST, the immobility time was (86.3±25.9) s. Compared with the control group, the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba(1∶1) in high doses (32 g·kg-1) had significant difference in TST and FST test (P<0.01). In addition, the results of the TST were in agreement with those in the FST. Conclusion: The results suggested that the coupled drug dispensing of Bupleuri Radix and Paeoniae Radix Alba, single use of Bupleuri Radix and single use of Paeoniae Radix Alba in different doses performed antidepressant-like effect, while there was difference in the antidepressant-like effect.关键词：Bupleuri Radix;Paeoniae Radix Alba;antidepressant;tail suspension test;forced swimming test- 2
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发布时间：2024-01-04 - 摘要：Objective: To study the acute toxicity, inheritance toxicity and sub-acute toxicity of coenzyme Q10. Method: The acute oral toxicity test,inheritance toxicity test including Ames test,polychromatic erythrocytes micronucleus test and sperm malformation test of coenzyme Q10 were carried out in mice and 30-day feeding test in rats was also conducted according to 《technical standards for testing ＆ assessment of health food》. Result: The result of acute toxicity in mice exceeded 20 000 mg·kg-1 and all the results of inheritance toxicity test were negative. During the 30-day feeding experiment the rats grew well and there were no significant differences in body weight and food consumption compared with the control group. At the end of the experiment,compared with the control group,there were also no significant differences in blood and biochemical examination,The histopathological changes in liver,kidney,stomach,intestines,spleen,testicle and ovawy were not observed in every group. Conclusion: Coenzyme Q10 is in non-toxic class; no inheritance toxicity and sub-acute toxicity were observed.关键词：coenzyme Q10;acute toxicity;genotoxicity;sub-acute toxicity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Jiangxiang Shuxin capsules on vascular endothelium in patients with unstable angina pectoris (UAP) of Qi stagnation and blood stasis type. Method: A design of randomized, placebo-controlled, double-blind trial was adopted; 158 cases of UAP of Qi stagnation and blood stasis type were selected and randomly divided into treatment group and control group, with 81 cases in treatment group and 77 in control group. The best western medical therapy solution was applied to both groups, on which basis Jiangxiang Shuxin capsules and placebo capsules was used respectively; daily 3 times, each time 4 grain. The course of treatment was 8 weeks. To observe improvements of angina, comparison of symptom-complex and the levels of serum NO, endothelin-1(ET-1), von willebrand factor(vWF), superoxide dismutase(SOD), malondialdehyda(MDA) from before treatment to after 8 weeks of treatment were observed, and the flow-mediated dilatation (FMD) in brachial artery was detected at the same time using ultrasonography. Result: The improvements of angina and total efficacious rate in treatment group was 93.82% and 98.76%,and control group was 68.83% and 66.23% respectively,the improvement of traditional Chinese medicine syndromes of the scores and total scores forchest pain,chest tightness,breathe hard,palpitations after treatment were lower than those before treatment in the two groups (P<0.01); score changes in treatment group were superior to that in control group (P<0.05); after treatment, the blood level of vWF and ET obviously decreased ( P<0.01), levels of NO and FMD increased (P<0.01) in both groups. Levels of SOD significantly increased and MDA signif icantly decreased in the treated group (P<0.01), while in the control group, no marked change was found in SOD and MDA. Conclusion: The results suggest that Jiangxiang Shuxin capsules was safe more effective,which could improve symptoms and the vascular endothe1ial function in patients with UAP of Qi stagnation and blood stasis type.Its mechanism might be associated with antioxidation in UAP.关键词：unstable angina pectoris;Qi stagnation and blood stasis type;endothelial function;Jiangxiang Shuxin capsules
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发布时间：2024-01-04 - 摘要：Objective: To explore the content changes of serum superoxide-dismutase (SOD) and malondialdehyde (MDA) of chronic superficial gastritis spleen-stomach vacuity cold(CSG-ssvc) patients by moxibustion on ginger therapy of differents moxibustion time. Method: Under the random, contrast and single-blind research method, 45 cases who conform to the standard of CSG-ssvc were randomly divided into three groups: the therapy group A (Moxibustion on ginger to the acupoints with 20 minutes) with 15 cases, the therapy group B (moxibustion on ginger to the acupoints with 40 minutes) with 15 cases and the medicine group C (taking medicine of ranitidine) with 15 cases. Moxibustion on ginger to the patients of therapy groups 1 time per day, and the patients of medicine group took ranitidine 0.15 g, 2 times per day. Three groups were continuous insist on 10 days for 1 treatment course, took a rest for 1 day and took 2 courses by all. The contens of Anti-oxidation factors were taken by draw blood and test serum on the first day and the 23 rd day. Result: ①All the three groups contents of SOD were significantly augmented after 2 treatment courses,there was a statistically difference when compared to the contents before treatments(P<0.05). And there was a statistically difference when compared to matched the changes of group B and group A, C(P＜0.05). ②All the three groups contents of MDA were significantly decreased after 2(P<0.05), treatment courses,there was a statistically difference when compared to the contents before treatments but there was no statistically difference compared with three groups. Conclusion: It can increase the contents of SOD and decrease the contents of MDA with both Moxibustion on ginger therapy and taking ranitidine. Moxibustion on ginger therapy for 40 minutes can increase SOD contents significantly,and the results are obviously higer than Moxibustion on ginger therapy for 20 minutes group and taking ranitidine group. Confirmed that Moxibustion on ginger therapy for CSG-ssvc patients has benign regulative the function of enzyme system of produce and remove the oxygen radical. This mechanism may be relevanted to the clinical effect of Moxibustion on ginger therapy for CSG-ssvc patients.关键词：Moxibustion on ginger;chronic superficial gastritis;spleen-stomach vacuity cold;superoxide-dismutase;malondialdehyde
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical therapeutic effect of Caiqin Shengjiangsan Jiawei decoction combination psychotherapy on general anxiety, to explore the effects of the electroencephalogram tests were recorded in a general nxiety. Method: A total of 60 patients with general anxiety were randomly divided into study group(30 case)and control group(30 case).The control group was treated with psychotherapy combination Caiqin Shengjiangsan Jiawei decoction treatment.The study group was treated with only given anxiety drugs. The treatment course was 6 weeks.Effects were evaluated with hamilton anxiety scale(HAMA) and the electroencephalogram tests were determined before and after treatment for 6 weeks. Result: After treatment,HAMA scores of two groups were decreased (P＜0.05); And between two groups before and after treatment,the difference of HAMA scores were not statistically significant. The frequency of α waves and θ,α brain electrical activity were significant between before and after treatment(P<0.05). there were no significant differences between the two groups in electroencephalo gram(EEG) and HAMA score,but there was no adverse reaction of the test group. Conclusion: Caiqin Shengjiangsan Jiawei decoction combination psychotherapy had effect in general anxiety.关键词：general anxiety;Caiqin Shengjiangsan Jiawei decoction;psychotherapy;electroencephalogram
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发布时间：2024-01-04 - 摘要：Objective: Explore the effects of Danhong injection on protect myocardium of acute coronary syndrome(ACS) patients after selective percutaneous coronary artery intervention(PCI). Method: Using a prospective design of experiments, the 68 patients of the intended line of PCI with ACS were randomly divided into treatment group and control group,each group is 34 patients. At the same time into the hospital health examination in 40 cases as health group.Observation the level of all patients of plasma endothelin-1(ET-1), P-selectin (CD62p), high enzyme C reactive protein (hs-CRP) and after treatment of 7 d ST segment resolution rate at PCI preoperatively, immediately after operation, treatment of 14 d. Using SPSS 9.0 statistical software for data statistics and analysis. Result: ACS patients preoperative plasma levels of ET-1, CD62p, hs-CRP were higher than the healthy group, The differences were statistically significant(P＜0.05). The treatment group compared with the control group, the difference was not statistically significant 14 days after treatment, two groups of patients with ET-1, CD62p, hs-CRP levels were decreased compared with those before treatment, the difference was statistically significant(P<0.05); The treatment group compared with the control group, treatment group, ET-1, CD62p, hs-CRP were lower than the control group, the difference was statistically significant(P<0.05). After treatment of 7 d, the treatment group ST segment resolution higher than that in the control group, the difference was statistically significant (P<0.05). Conclusion: Danhong injection can inhibit ASC in patients with PCI postoperative inflammatory reaction, protection of endothelial cells, is proposed as PCI postoperative myocardial protection measures in clinical treatment关键词：acute coronary syndrome;percutaneous coronary intervention;Danhong injection
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of total Saponin of Radix Clematidis (TSRC) on HL60 leukemia cell in vitro. Method: In vitro, HL60 cells were incubated with 25-400 mg·L-1TSRC,the proliferation inhibition rate of HL60 was tested by WST-1.Cell morphology,transmission electron microscope(TEM) and Annexin-V/PI were used to investigate the apoptosis of HL60 cells. The differentiations of HL60 was analyzed by morphology of Giemsa stain,nitroblue tetrazdium(NBT) reduced method. The expressions of CD11b and CD33 induced by these drugs were measured by flow cytometry(FCM) at different time. Result: As compared with blank control group, Different concentrations from 50-400 mg·L-1 TSRC could obviously inhibit the growth of HL60 cells with time and dose dependence; While no statistical significance in the differentiation phenomenon was observed. Conclusion: In vitro, HL60 cell’s proliferation inhibition and apoptosis could obviously induced by TSRC. But HL60 cells could not be induced differentiation by TSRC.关键词：total saponin of Radix Clematidis(TSRC);acute promyelocytic leukemia(APL);HL60 cell;proliferation;differentiation;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of combined Chinese and western medicine therapy on acute exacerbation of chronic pulmonary heart disease (CPHD). Method: One hundred and eleven cases (CPHD) with acute exacerbation were randomly divided into 56 cases of the observation group and 55 cases of control group (western medicine group). Conventional western medicine treatment combined with Qingjin Huatan decoction and Xuefu decoction Tang was applied to the observation group, one a day orally for 12 days. Cardiac function, pulmonary artery pressure, arterial blood gas analysis, hemorheological parameters and 6 minutes walking distance were observed. Result: The efficacy of the cardiac function in the observation group was 92.85%, better than that (72.72%) in the control group (P<0.05). Pulmonary artery pressure, arterial blood gas analysis and hemorheological parameters in the observation group were improved better than those in the control group (P<0.01).The walking distance within 6 minutes in the observation group was longer than that in the control group (P<0.01). Conclusion: Combined Chinese and western medicine therapy can effectively reduce pulmonary artery pressure, increase 6 minutes walking distance, reduce blood viscosity, improve heart and lung function for patients with CPHD, and eventually improve the clinical outcome.关键词：chronic pulmonary heart disease;acute exacerbation;Qingjin Huatan decoction;Xuefu Zhuyu Decoction;pulmonary hypertension;hemorheology;6 minute walking test
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Xueshuan Xinmaining capsule in patients with coronary heart disease and impact on interleukin (IL-6), tumor necrosis factor (TNF-α) and high-sensitivity C-reactive protein (hs-CRP). Method: Sixty-five patients with coronary heart disease were randomly divided into treatment group of 33 cases and control group of 32 cases. Both groups were given a conventional treatment, the treatment group was additionally given Xueshuan Xinmaining capsule, 4 grain tid for 8 weeks. The level of IL-6, TNF-α, hs-CRP, ECG and the count of angina pectoris were observed. Result: For angina pectoris, the total effective rate of treatment group was 90.9%, better than the control group of 56.3% (P<0.01). The electrocardiogram total effective rate in treatment group was 84.8%, better than the control group of 56.3% (P<0.01). After treatment, the serum IL-6, TNF-α and hs-CRP in treatment group were significantly lower than those in the control group (P<0.01). Conclusion: Xueshuan Xinmaining capsule can reduce the count angina pectoris in patients, improve patients' ECG and reduce IL-6, TNF-α and hs-CRP level. Its mechanism may be related to its anti-inflammatory action.关键词：coronary heart disease;Xueshuan Xinmaining capsule;IL-6;TNF-α;hs-CRP
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Acupoint injection combined with Chinese herbal medicine on cervical vertebra disease of vertebral artery type. Method: Eighty cases of cervical vertebra disease of vertebral artery type were divided into two groups of 40 cases randomly. The treatment group was treated with acupoint injection (Compound Danshen injection) combined with oral Chinese herbal medicine (Banxia Baizhu Tianma decoction), and the control group was treated with Chinese drugs orally (Banxia Baizhu Tianma decoction), then the efficacy of two groups was compared. The blood flow velocity in vertebral artery (VA), basilar artery (BA) of 2 groups after treatment was measured by transcranial Doppler (TCD). The levels of nitric oxide (NO) and endothelin (ET) in plasma were also determined. Result: Total effective rate of the treatment group was 95.00%, and total effective rate of the control group was 77.50%. There was significant difference between two groups (P<0.01). The blood flow velocity in VA, BA of 2 groups after treatment was significantly faster than those before treatment (P<0.05). The VA blood flow velocity of treatment group, compared with the control group, was significant high (P<0.05). The NO level in the treatment group was high than that in the controls, and the level of ET in the treatment group was low than that in the controls (P<0.05,P<0.01). Conclusion: Acupoint injection combined with Chinese herbal medicine is especially effective for treatment of cervical vertebra disease of vertebral artery type.关键词：cervical vertebra disease;acupoint injection;compound Danshen injection;Banxia Baizhu Tianma decoction
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical therapeutic effect of the combination of Wenglitong capsule and western medicine in the treatment of benign prostatic hyperplasia. Method: Fifty two cases of control group treated by western medicine,48 cases of treatment group treated by the combination of Wenglitong capsule and western medicine, international prostate symptom sore(IPSS),quality of life(QOL), maximum flow rate (MFR), volume(V),white blood cell(WBC) were compared between two groups in the pre-theatment and had been theated 12 weeks. Rusult: IPSS,QOL,MFR,WBC of two groups presented siginificant statistical difference in comparison. Treatment group superior to control group. The total effective was 94% in treatment group, the total effective was 85% in control group. There were no difference on V. Conclusion: The combination of Wenglitong capsule and western medicine in the treatment of benign prostatic hyperplasia can improve more obviously impacts and quaulity of life than by western medicine only.关键词：Wenglitong capsule;Benign Prostatic Hyperplasia;Dysuria
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发布时间：2024-01-04 - 摘要：Objective: To investigate the selective effect of Xihuang Pill on-estrogen receptor status of breast cancer. Method: Thirty estrogen-dependent breast cancer patients and 30 non-estrogen-dependent breast cancer patients were respectively randomLy assigned to two groups by digital table. The estrogen-dependent breast cancer patients used Letrozole 2.5 mg·qd. for 30 d and the treatment one was added with Xihuang Pill 3 g,bid for 30 d. The treatment group of non estrogen-dependent breast cancer patients were treated with Xihuang Pill. All the patients were observed their Traditional Chinese Medicine (TCM) syndrome, quality of life and tumor mark of breast cancer (CA50,CEA,CA153s)as well. MTT assay was used to assessed the viability of human breast cancer cell lines MCF-7 and MDA-MB-231 submitted to Xihuang Pill drug serum. Result: The treatment group and control group of estrogen-dependent breast cancer patients in improvement of quality of life was respectively 60%, 40% (P<0.05), and the total improvement rate of TCM syndrome was respectively 73.3%, 53.3% (P<0.05). While the non estrogen-dependent one quality of life was respectively 46.7%, 33.3%(P<0.05), and the total improvement rate of TCM syndrome wais respectively 66.7%, 46.7% (P<0.05). The CA153 values of treatment group and the control group of estrogen-dependent breast patients had a significant difference (P<0.05). Xihuang pill drug serum slightly decreased MCF-7 proliferation in the serum concentrations of 6.25%-25% in vivo, but promote proliferation of MDA-MB-231 instead. Conclusion: Xihuang pill had attenuated synergistic effect on estrogen-dependent breast cancer combined with Letrozole. And the curative effect was better than that of non-estrogen-dependent breast cancer, which showed its selective effect.关键词：Xihuang pill;breast cancer;estrogen receptor;comparative study
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effects on the treatment of local retinal detachment after laser therapy with self-prescribed strengthening spleen and promoting urination prescription. Method: Not more than 1 quadrant of t local retinal detachment in 71 patients (72 eyes). The Argon laser therapy with photocoagulation 2-3 laps at the outer edges of the retinal detachment zone.The patients were randomly divided into two groups, control group without drug treatment, the treatment group with Self- prescribed strengthening spleen and promoting urination prescription treatment 2-3 weeks, 1 year follow-up observation two groups of subretinal effusion absorption. Result: Control group 36 patients (36 eyes) 13 eyes cured, 15 eyes effective, 8 eyes is invalid. The treatment group 35 patients (36 eyes) 22 eyes cured, 13 eyes effective, 1 eye is invalid. Add the strengthening spleen and promoting urination prescription had better effects than control group, the difference was statistically significant (P<0.05). Observe the subretinal effusion absorption time, the treatment group significantly shorter than in the control groups. Conclusion: Laser for the treatment of localized retinal detachment is to avoid surgery safety and effective method, add to the traditional Chinese medicine strengthening spleen and promoting urination prescription treatment can significantly improve the effect of laser treatment.关键词：strengthening spleen and promoting urination prescription;local retinal detachment;laser treatment
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发布时间：2024-01-04 - 摘要：According to the literatures on the study of Compound Kushen Injection published in recent years. The advance on its chemical constituents, quality control , drug metabolism, pharmacological activities and clinical applications were summarized. The main constituents of Compound Kushen Injection are alkaloids, including oxymatrine and matrine, and the injection has the diverse activities of anti-tumor,analgesia and raising tumor patient's immunity. It was widely used for the adjuvant therapy of end-stage cancer. It should be further investigated for the quality consistency and the effectiveness as well as the safety of the product.关键词：compound Kushen injection;chemical constituents;quality control;pharmacological activities;clinical applications
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发布时间：2024-01-04 - 摘要：To investigate literature in the world on the chemical and pharmacological studies on the genus Onychium. To review the progress on chemical and pharmacological studies through the analysis of literature on plants of the genus Onychium. Up to now, more than 30 chemical components were isolated from Onychium plants including flavonoids, triterpenes, diterpenes, sesquiterpenes, and so on. Some compounds exhibited protection of vascular endothelial cells (VECs), antitumor, antioxidant and antivirus activities. Studies on the plant would have important significance to the finding of new bioactive compounds.关键词：Onychium;onychin;antitumor
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发布时间：2024-01-04 - 摘要：This study aims to summarize the research advances on composition, extraction, detection, physiological activity and provides scientific foundation for the development and utilization of flavonoids from L.japonicae Flos in last five years.The methods of flavonoids extraction from L. japonicae Flos are more mature than separation and purification which are relatively less. Its main content determination is by ultraviolet spectrophotometry,and HPLC is second. The physiological activity of antioxidant,antibacterial and liver protective effect have been reported,so more researches need medical workers to be done is to explain its protect cardiovascular,digestive system,anti-inflammatory and anti-immune,antitumor and analgesic effects.关键词：Lonicerae Japonicae Flos;flavonoids compounds;extraction;detection;physiological activity.
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发布时间：2024-01-04 - 摘要：Objective: Collation and analysis of literatures about traditional Chinese medicine(TCM) treatment on hyperplasia of mammary gland(HMG) by searching Wanfang data and CNKI, we found 242 articles from1996 to 2011,then we analyzed and summarized 35 of them. The results show TCM has obvious advantage on HMG therapy, yet lack of modern evidence-based medicine guiding ideology from the experiment research to the clinical treatment. In the future, we should regulate the design of experiments, deepen the research work to realize the significant breakthrough on Chinese medicine treatment of HMG.关键词：hyperplasia of mammary gland;treaament of TCM;literature sorting
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发布时间：2024-01-04 - 摘要：We summarize species analysis of mercuric sulfide by consulting documents, also discuss how the forms of mercuric sulfide influence on the toxicity and pharmacological function. Recent evidence suggests that mercuric sulfide in cinnabar and vermilion is red crystallization of optical activity, insoluble Hg2+, soluble Hg2+, Hg+are storage forms of mercury sulfide in vitro, and insoluble Hg2+is the main form. The main toxicity of mercuric drugs is caused by soluble mercury. Mercuric sulfide acts as main component of many tradition drugs, these drugs may exist as polysulfide. This existence form will reduce its toxicity. Pharmacological research shows that clinical dosage usually does not cause people poisoned. We should establish standards through modern analysis in order to ensure the safety of mercury-containing drugs.关键词：mercuric sulfide;species analysis;physicochemical properties;pharmacology
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发布时间：2024-01-04 - 摘要：To study the Herbalism study of the Sow thistle, verify its name and origin, and clarify the confusion of literature records, in order to further utilize its resources. Refer to the literature and its application history combining with the fieldwork. The main source of the Sow thistle are the plants of Sonchus oleraceus L. Ixeris denticulata (Houtt.) Stebb. And I. chinensis (Thunb.) Nakai. etc. coming from Compositae. Sow thistle medicinal varieties of confusion. It’s name, origin and characteristics described diversity in the materia medica and modern literature,and the presence of homonyms phenomenon, so we should reform from the bottom, and provide scientific basis for its development and utilization.关键词：sow thistle;textual research;origin
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发布时间：2024-01-04
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