最新刊期
卷 18 ， 期 19 ， 2012
- 摘要：Objective:Evidence Based Medicine (EBM) is used to do systematic reviews on Shegan Mahuang tang (Ephedra decoction) controlling cough variant asthma (CVA) in children. Method:Data retrieval is done on the websites such as cnki, cqvip, wanfangdata and offline professional magazines. Result:Ninty-four pieces of literatures are attained and 7 pieces accord with the inclusive criteria. Jadad Criteria is adopted to assess every literature's Methodological quality, all of which are low quality ones. 470 cases are obtained. OR=2.99, 95% CI of 1.90 to 4.71, test for overall effect:Z=4.71(P<0.000 01). Therefore, the difference is statistically significant. Conclusion:The existed clinical evidences shows, Shegan Mahuang tang is effecitve to control cough variant asthma (CVA) in children. However, randonmized control clinical trials are subsumed by systematic reviews belong to low quality ones, so Shegan Mahuang tang's therapeutic effect need to be further examined by a large scale of high quality of clinical trials.关键词：Shegan Mahuang tang (Ephedra decoction);cough variant asthma (CVA) in children;systematic reviews;Meta analysis
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发布时间：2024-01-04 - 摘要：Objective:To classify traditional Chinese medicines in treatment of the hand foot and mouth disease(HFMD) to provide a new way for the further study on the formula rules and clinical application of these traditional Chinese medicines. Method:The data were collected from January, 2000 to December, 2010.From more than 126 Chinese herbal compounds which were used to 20 cases effectively and havd 10 or more frequency,40 compounds were chosen. Based on the property of a medicine including the channel tropism and the effect of multiple variables,all the paper were published in the journals. The SPSS 19 software package was used, and the distribution of traditional Chinese medicines were dealt with cluster and correlative analysis, which were based on fuzzy mathematics. Result:Through clustering method,different categories were separated, using the principle of optimization,The data were divided into 4, 6 and 8 categories. The cluster analysis showed that 4 categories was better. The four kinds of medicine were belongs to heat clearing, diaphoretic, resolving dampness drug and eliminating dampness through diuresis medicine. Conclusion:The cluster analysis method can be used to classify different classes and havs similar efficacy of traditional Chinese medicines.There is a relation between the treatment of Hand Foot and Mouth Disease and the theory of traditional Chinese medicine,which is significant for the further study on clinical application of these traditional Chinese medicines.关键词：traditional Chinese medicine therapy;hand foot and mouth disease;clustering analysis
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发布时间：2024-01-04 - 摘要：Objective:To analyze the principles of prescriptions for chronic heart failure by using deta mining technology based on Traditional Chinese Medicine Inheritance System. Method:The retrospective analysis of 272 patients with chronic heart failure treated by traditional Chinese medicine in two Three-A hospitals of Beijing by using the data mining methods, such as revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering, etc. Result:Seventy-eight kinds of herbs were used frequently in clinical and 32 drug collocation correlative coefficients are more than 0.02; 26 core combinations and 13 new prescriptions were mined from the database. Conclusion:Traditional Chinese Medicine Inheritance System is a helpful tool on entrying, administration and analyzing of the prescriptions and it has importance for creating new prescriptions.关键词：chronic heart failure;deta mining technology;Traditional Chinese Medicine Inheritance Support System;composition principle
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发布时间：2024-01-04 - 摘要：Objective:To analyze the composition principles of the external use prescriptions for eczema collected in 〈Prescription Dictionary of Traditional Chinese Medicine〉. Method:The external use formulas used for eczema in 〈Prescription Dictionary of Traditional Chinese Medicine〉 were collected, then entered the Traditional Chinese Medicine Inheritance Support System (TCMISS;V1.1). By using data mining methods, composition principles of the prescriptions were analyzed. Result:Based on the analysis of 170 cases of external use formulas, the frequency of each herb included in the database was computed, 42 core combinations and 4 new prescriptions were mined from the database. Conclusion:The composition principles of the formulas for eczema collected in were summarized. This would be the foundation for further research and development of new drugs for treating eczema.
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发布时间：2024-01-04 - 摘要：Objective:To investigate influence of different packaging materials and different storage state on quality stability of Rehmannia glutinosa pieces, and establish the best storage conservation method of R. glutinosa. Method:Three different packaging materials were used,and R. glutinosa were stored at three different temperatures and two kinds of confined environment.With verbascoside as evaluation index, and HPLC fingerprint technology was used to evaluate impact of different packaging materials, different storage on quality stability of R. glutinousa pieces. Result: On conditions of PTP aluminum foil,vacuum and refrigeration had the highest content of verbascoside,which were (0.028±0.011)%,(0.026±0.016)%,(0.025±0.014)%,respectively.The content of verbascoside in descending order as follows:PTP aluminum foil>polyethylene plastic bag>leather condensate film paper; vacuum>unvacuum; refrigerated>cool conditions>normal temperature.Optimum storage and curing condition was:PTP aluminum foil packaging,vacuum refrigeration. Conclusion: In this optimized condition,R. glutinousa pieces was stable,it could provide experimental basis for storage in actual production of R. glutinousa pieces.关键词：Rehmannia glutinousa;packaging materials;storage state;fingerprint;stability
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction conditions of Danggui Shaoyao powder. Method:With the content of albiflorin,paeoniflorin,ferulic acid and 23-acetate alisol B as indexes,test data was handled by multiple-index comprehensive scoring method,orthogonal test was adopted in optimizing extraction technology with extraction time,extraction times,the concentration of ethanol and ethanol volume as factors. Result:Optimized extraction technology was:extracted 2 times with 8 times the amount of 75% ethanol,1.5 h per time. Conclusion:This optimized extraction technology was rational and stable with high yield of active components.关键词：Danggui Shaoyao powder;albiflorin;paeoniflorin;ferulic acid;23-acetate alisol B;orthogonal test;multi-index comprehensive scoring method
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发布时间：2024-01-04 -
Optimization of Enrichment and Purification Technology for Total Glycosides fromby Macroporous Resin
摘要：Objective:To optimize technology conditions and technical parameters of enrichment and purification process for total glycosides from Acanthopanax giraldii by macroporous resin. Method:A RP-HPLC method was adopted to determine the content of total glycosides in A. giraldii with elution ratio of syringin and oleanolic acid as indexes,adsorption characteristics and elution parameters of enrichment and purification technology were investigated from 10 kinds of macroporous resin. Result:X-5 was the best macroporous resin;Optimum purification technology was:the concentration of total glycosides extraction from A. giraldii 0.3 g·mL-1,pH of liquid 10, eluted with 4 BV 80% ethanol. Conclusion:X-5 macroporous resin could be well used for enrichment and purification of total glycosides from A. giraldii.关键词：Acanthopanax giraldii;total glycosides(TGA);macroporous resin;RP-HPLC- 2
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发布时间：2024-01-04 - 摘要：Objective:To investigate effect of different boiling conditions on quality of Buyang Huanwu decoction by high-pressure decoction machine, and optimize boiling process. Method:With total solid content, the content of ferulic acid and astragaloside as indexes,orthogonal test was used to analyze effect of four factors including soaking time,the amount of water,boiling time and boiling temperature.The content of ferulic acid and astragaloside were determined by HPLC. Result:Orthogonal test analysis of variance showed that order of influence factors:the amount of water>boiling temperature>soaking time>decoction time,none of these factors had significant impact on extraction technology.Optimum boiling technology of Buyang Huanwu decoction was:medicinal pieces soaked 30 min with 12 times the amount of water,decoction time 60 min,boiling temperature of 120℃. Conclusion:This optimized technology and technical parameters provided theoretical basis for traditional Chinese medicine decoction with ferulic acid and astragaloside as main ingredients,which was prepared by high-pressure decoction machine.关键词：Buyang Huanwu Decoction;high-pressure decoction machine;orthogonal test;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To optimize water extraction technology of Qijiao ointment. Method:With astragaloside and water extracts as indexes,HPLC-ELSD was used to determined the content of them,water extraction technology was optimized by orthogonal design,extraction times,extraction time and the amount of water were investigated as factors. Result:Optimal water extraction process of Qijiao ointment was:added 8 times the amount of water,decocting for three times,1 h each time. Conclusion:This optimized technology was stable and feasible,it showed high yield of astragaloside in Qijiao ointment.关键词：orthogonal design;astragaloside;HPLC-ELSD;Qijiao ointment
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发布时间：2024-01-04 -
Preparation and Quality Control of Oral Disintegrating Tablet with Extracts of Total Flavonoids from
摘要：Objective:To prepare oral disintegrating tablet with extracts of total flavonoids from Zea mays, and establish its quality control method. Method:Tablets were prepared by direct compression from powder, Single factor test was employed to investigate pressure,lubricants and flavoring agent;With disintegration time as index, disintegrating agent was optimized by orthogonal design.Its quality was investigated with dissolution and content uniformity as indexes. Result:Optimized formulation was as follows:extracts of total flavonoids from Z. mays 23%, cross-linked polyvinylpyrrolidone 15%,microcrystalline cellulose 35%,orange flavor-mannitol-aspartame 1.2:5:2, These prepared tablets could disintegrate completely within 30 s and had good taste.Dissolution and content uniformity were in line with requirement of 2010 edition of ‘Chinese Pharmacopoeia'. Conclusion:This preparation and quality control were simple and feasible.关键词：total flavonoids from Zea mays;orally disintegrating tablet;disintegration time;quality control- 2
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发布时间：2024-01-04 - 摘要：Objective:To optimize water boiling technology of Bazhen decoction. Method:With the content of paeoniflorin, total polysaccharides and extracts as indexes,Orthogonal design was performed to investigate influence of soaking time, decocting time, decocting times and the amount of water on water boiling technology. Result:The best decocting progress was:soaked 30 minutes,add 10 times amount of water,decocted 2 times and 30 minutes per time. Conclusion:This optimized process was stable and reasonable, it could provide a reference for production of Bazhen decoction.关键词：Bazhen decoction;paeoniflorin;total polysaccharides;water boiling process;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective:To optimize accessories and its dosage of compound Xuelian capsule. Method:With moisture percentage as index,orthogonal test was used to optimize accessories from three kinds hydrophobic property excipients (magnesium stearate,magnesium oxide and calcium hydrogen phosphate),and to investigate its dosage. Result:Optimized accessories of compound Xuelian capsule was magnesium stearate and its dosage is 1.5%. Conclusion:Hygroscopicity of compound Xuelian capsule was improved after added hydrophobic accessories, it adapted to actual production.关键词：compound Xuelian capsule;accessories;hygroscopicity
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发布时间：2024-01-04 - 摘要：Objective:To discuss influence of oven baking on double and single ester type alkaloids from Aconitum carmichaeli. Method:A. carmichaeli were processed as follows:cut into slices→protect color→hot drift→baking,with the content of double and single ester type alkaloids as evaluation indexes, orthogonal test was used to investigate influence of hot drift time, baking temperature, baking time on the content of two type alkaloids. Result:With increase of each factor level, the content of double ester type alkaloids decreased,but the content of single ester type alkaloids increased.In addition, the content of single ester type alkaloids in ten products met requirements of the ‘Chinese Pharmacopoeia' of black slice, white slice, and dan slice, the content of double ester type alkaloids in six products met requirements of the ‘Chinese Pharmacopoeia' of sold black slice and white slice. Conclusion:The content of double and single ester type alkaloids could met limited requirements of the 2010 edition ‘Chinese Pharmacopoeia' by oven baking.关键词：Aconitum carmichaeli;oven baking;orthogonal test;double ester type alkaloids;single ester type alkaloids
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology conditions of volatile oil from Ligusticum chuanxiong, Angelica sinensis and Cinnamomum cassia in Guanxinkang capsule. Method:Volatile oil from L. chuanxiong, A. sinensis and C. cassia were extracted by steam distillation method,with yield of volatile oil as evaluation index, the amount of water,soaking time and extraction time were investigated by central composite design,test data was fitted by multi-linear equation and second-order polynomial equation,extraction technology conditions were optimized by response surface method,and to predictive analysis. Result:Multiple correlation coefficients from second-order polynomial equation were prior to those from multi-linear equation,optimum extraction process conditions were as follows:soaked 80 min with 9 times the amount of water,distillation time 7.5 h,deviation between observed and predicted values was less than 3%. Conclusion:This optimized process was simple with high predictability, it could provide test basis for industrial production of Guanxinkang capsule.关键词：Guanxinkang capsule;volatile oil;central composite design-response surface methodology;extraction technology
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发布时间：2024-01-04 - 摘要：Objective:To optimize wine-steaming processing technology of Astragahlus complanatus. Method:With the content of complanatoside and total flavonoids,water extracts as indexes,orthogonal design was used to investigate influence of moistening time,steaming time and the amount of wine on wine-steaming technology of A. complanatus. Result:The best processing technology was:moistening 1 h,steamed 3 h,the amount of wine 30%. Conclusion:This optimal rice wine-steaming processing technology of A. complanatus was stable and feasible,it could provide experimental basis for quality control of A. complanatus.关键词：Astragahlus complanatus;wine-steaming;content determination;orthogonal design
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction process of main components from flower of Gnaphalium hypoleucum. Method:With the contents of quercetin and apigenin as comprehensive evaluation index,which were determined by HPLC.Extraction method,ratio of solid-liquid,extraction times and the concentration of ethanol were investigated by single factor test;orthogonal design test was used to optimize extraction technology with extraction time,extraction times,ratio of solid-liquid and the concentration of ethanol. Result:Optimum extraction conditions were as follows:ultrasonic extracted 2 times with 30 times the amount of 60% ethanol,40 min per time. Conclusion:This optimized process was simple,stable and feasible.It could provide reliable extraction method for quantitative analysis of main components from flower of G. hypoleucum.关键词：flower of Gnaphalium hypoleucum;quercetin;apigenin;Orthogonal Design;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To investigate and optimize preparation technology of Gefen instant granule. Method:With dissolution time and the content of total flavonoids of this granule as indexes,ratio of sugar- Pueraria lobata,ratio of baking powder-P. lobata, drying temperature were chosen as investigation factors,preparation technology was optimized by orthogonal test with Z-comprehensive analysis method.Three samples were prepared by optimized technology,and their properties,melting,water content and pasting test were investigated.The content of total flavonoids was determined by aluminum nitrate chromomeric method. Result:The best preparation process was as follows:uniform mixed baking powder 1.2 g, dextrin 1.8 g, sugur 24 g, and P. lobata 33 g sequentially by equal increment method,mixed evenly with about 5 mL hot water (35-40℃),shaped into lump, placed at environment of 35℃ for 40 min to make its interior with multiple and dense holes, volume expansion and soft texture,squeeze sieved,dried at 60℃ for 40min, arranged granules, and then Gefen instant granule were gained.Prepared 3 batches of samples had uniform size, milky white, color consistency, no absorption of moisture, agglomeration and phenomenon of deliquescence.Melting test qualified,it dissolved completely at about 85℃ in hot water.The moisture content was less than 6.0%, and the content of total flavonoids was 0.327 mg·g-1. Conclusion:This optimized preparation technology was simple, feasible and reproducible, and the content of total flavonoids determination method was suitable for quality control of Gefen instant granule.关键词：instant granule;Z-comprehensive scoring method;melting time;total flavonoids
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发布时间：2024-01-04 - 摘要：Objective:To optimize fried extraction process of Arnebia euchroma from Yuhong ointment. Method:With yield of hydroxyl naphthalene quinones total pigment as index,single factor test was performed to investigate kind of moistening solvent and the concentration of moistening solvent; UV-spectrophotometry was applied to determine the content of hydroxyl naphthalene quinones total pigment,fried extraction technology of A. euchroma was optimized by uniform design,which was used to investigate influence of moistening solvent concentration,solvent dosage,fired temperature and fired time on extraction process. Result:The best fried extraction technology was satisfied with some conditions as follows:moistened with 3.2 times the amount of 68.87% ethanol, fired temperature 160.15℃, fired time 1.96 min. Conclusion:This optimized extraction process was reasonable and stable, it could provide test basis for traditional fried extraction process of A. euchroma.关键词：Yuhong ointment;Arnebia euchroma;uniform design;extraction process;hydroxyl naphthalene quinones total pigment
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发布时间：2024-01-04 - 摘要：Objective:To optimize enrichment and purification technology of total flavonoids from Salvia plebeia. Method:With adsorption-desorption ratio and transfer ratio as indexes,optimum macroporous resin was selected by static adsorption-desorption ratio tests;The concentration of eluting agent was determined with the content of total flavonoids as index;Purification technology was optimized with impurity rate,transfer rate of homoplantaginin and transfer rate of total flavonoids as indexes. Result:D101 was selected for its excellent adsorption and desorption properties,optimum process was as follows:the amount of crude drugs to D101 macroporous resin 1 g·mL-1,eluted with 7 BV 75% ethanol,elution flow rate 6 mL·min-1. Conclusion:Total flavonoids from S. plebeia could be effectively purificated and separated by D101 macroporous resin.关键词：total flavonoids from Salvia plebeia;Macroporous resin;purification technology
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发布时间：2024-01-04 - 摘要：Objective:To prepare folate-conjugated hyaluronic acid-octadecyl (FA-HA-C18) polymeric micelle for paclitaxel loading. Method:FA-HA-C18 copolymer was prepared by the reaction of the activated folate ester with the carboxyl group on the hyaluronic acid. The chemical structure was identified by FTIR and UV techniques. The paclitaxel-loaded FA-HA-C18 polymeric micelle was prepared by ultrasonic method, and the preparing process was also optimized. The average particle size and zeta potential of the paclitaxel-loaded FA-HA-C18 micelle were determined. Result:The degree of substitution of folate in FA-HA-C18 copolymer was 6.8%. The average particle size and zeta potential of paclitaxel-loaded FA-HA-C18 polymeric micelles were (191.9±8.7) nm and -33.3 mV, respectively. Conclusion:FA-HA-C18 polymeric micelles could be used as a novel drug delivery system for paclitaxel.关键词：folate-conjugated hyaluronic acid-octadecyl(FA-HA-C18);Polymeric micelles;paclitaxel;ultrasonic method
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC method for simultaneous determination of dopamine hydrochloride and salsolinol in Radix Aconite Lateralis. Method:The separation was carried on an Agilent Eclipse C18 column(4.6 mm×250 mm,5 μm)with 0.1% H3PO4 as mobile phase at a flow rate of 1 mL·min-1. The column temperature was set at 35℃ and the detector wavelength was at 280 nm. Result:Dopamine hydrochloride and salsolinol were all well separated from other substances and their UV spectrums were essentially the same to the standards. The liner ranges of dopamine hydrochloride and salsolinol were 0.005 21-0.521 μg,0.005 24-0.524 μg. The average recoveries were 100.6% and 96.5%;RSD were 2.0% and 1.0% (n=6). Conclusion:This method is rapid and accurate and is suitable for quality control of Radix Aconite Lateralis.关键词：Radix Aconite Lateralis;HPLC;determination;dopamine hydrochloride;salsolinol
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发布时间：2024-01-04 - 摘要：Objective:To identify the differences and similarities of chemical components obtained through different extraction methods about Benzoinum and to explore the best extraction method to study the volatile components of Benzoinum. Method:The similarities and differences among solvent extraction (by petroleum ether and ethyl ether), extraction of volatile oils through steam distillation and extraction of volatile components through HS-SPME were analyzed and compared by the GC-MS technology. Result:5 compounds were identified through the extraction of the solvent of Benzoinum, including 4 compounds in the part of petroleum ether and 4 compounds in the part of ethylether. 19 compounds were identified during the volatile oils extracted by steam distillation. 12 compounds were identified through SPME. The components obtained through three methods all include 4 chemical constitutions such as benzoic acid, benzyl benzoate, vanillin and benzyl cinnamate. The steam distillation obtained the most components in this three extraction methods. Conclusion:The steam distillation obtained the most components in this three extraction methods. It suggested that the steam distillation is the best method to study the volatile components of Benzoinum.关键词：Benzoinum;solvent extraction;Steam Distillation;HS-SPME;GC-MS
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发布时间：2024-01-04 - 摘要：Objective:To compare the content of volatile oil and its chemical composition in Zingiberis Rhizoma recens, Zingiberis Rhizoma, Zingiberis Rhizoma praeparatum and Zingiberis Rhizoma carbonisata and evaluate the effect of the process on the volatile components in the ginger and its processed products. Method:The volatile oil was extracted with steam distillation. GC and GC-MS analysis were carried out to compare the chemical composition of the volatile oil in different samples. Result:The content of oils in different samples was as follows:Zingiberis Rhizoma recens>Zingiberis Rhizoma>Zingiberis Rhizoma praeparatum>Zingiberis Rhizoma carbonisata. The chemical components in four kinds of ginger products were same, and the difference is the ratios of the various components. Especially, the components with low boiling point in the volatile oil of fresh ginger are more than those in other three products. The main components, such as camphene, β-phellandrene, benzene,1-(1,5-dimethyl-4-hexenyl)-, α-trans-β-bergamotene and cyclohexene,3-(1,5-dimethyl-4-hexenyl)-6-methylene-, are present in all the samples of ginger products. Conclusion:Comparing with fresh ginger, the content of volatile oil was decreased due to the process procedure, especially, the content of the components with the low boiling point, however, the consistency of the chemical components is helpful for the establishment of the uniform quality standards of the different ginger products.关键词：ginger;process;volatile oil;GC;GC-MS
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发布时间：2024-01-04 - 摘要：Objective:To establish the determination method of quercetin in Chinese patent medicine and health-care product by carbon paste electrode(CPE)anodic adsorptive voltammetry. Method:The CPE was prepared by carbon powder and liquid paraffin(4:1), in a acetic acid-sodium acetate buffer solution(pH 3.6). Quercetin gives a sensitive adsorptive oxidation peak at 0.401 V(vs. Ag-AgCl). Result:The linear relationship between the peak current and the concentration was found between 3.13×10-7-1.0×10-5mol·L-1 with correlation coefficient of 0.996 0, and the detection limit was 2.1×10-8mol·L-1. The method was applied to the analysis of Chinese patent medicine and health-care product and has achieved good results. Conclusion:The method is accurate and reliable, economical and practical.关键词：quercetin;anodic adsorptive voltammetry;carbon paste electrode;ginkgo leaves tablet;quercetin multiple capsule
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC fingerprint of iridoids and crocetin derivatives in Gardenia Fructus (GF) for the evaluation of the intrinsic quality. Method:Chromatographic separation was carried out by an Inertsil ODS-SP column (4.6 mm×250 mm,5 μm) and gradient elution was performed by mobile phase containing MeOH and 0.1% formic acid aqueous solution. The flow rate was 1.0 mL·min-1, the column temperature was maintained at 25℃, and the separated compounds were detected at a variable wavelength. The similarity evaluation software was adopted to analyze the similarities of 10 batches of commercial GF. Result:A consistent HPLC fingerprint pattern containing 20 common peaks was obtained and two major components were identified as geniposide and crocin-1,and the results of similarities of ten batches were range from 0.95 to 0.99. Conclusion:The established fingerprint method which reflect more chemical composition information of GF had good precision, reproducibility, stability, and could provide as an effective tool for the quality control of GF.关键词：Gardenia Fructus;iridoids;crocetin derivatives;fingerprint;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the determination of estragole in anise oil. Method:The essential oil was extracted from Illicium verum and then analyzed by GC. the GC was fitted with HP-INNOWax capillary column (0.32 mm×30 m×0.50 μm) film thickness; flame ionization detector and operated under the following conditions:Nitrogen carrier gas; split injection 100:1; temperature programming. Result:The calibration curves were linear in the range of 0.08-0.32 μg for estragole,r=0.999 8,average recovery of estragole was 101.0%, RSD was 1.27%(n=9). Conclusion:The method is simple, accurate, reproducible and can be used as the quality control for Illicium verum.关键词：estragole;anise oil;GC
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发布时间：2024-01-04 - 摘要：Objective:The essay aims at making a comparative study on the total flavonoids content of several kinds of wild and cultivated Glycyrrhiza uralensis in different producing regions in Ningxia. Method:The content of total flavonoids was determined by UV. The total flavonoids of wild and cultivated G. uralensis in different producing regions in Ningxia were exmined and analyzed. Result:The determination method had good linear relationship in the ranges of 4.36-43.6 mg·L-1 for total flavonoids. As for the total flavonoids of wild G. uralensis in different producing regions, there is a remarkable differert in total flavonoids. The content of total flavonoids in wild was higher than that in cultivated in same producing regions, and the content in cultivated in dry land was higher than that in cultivated in irrigated land. Conclusion:It was important that the regional features influenced on the total flavonoids content of G. uralensis.关键词：Glycyrrhiza uralensis;total flavonoids;wild and cultivated;UV
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发布时间：2024-01-04 - 摘要：Objective:To determine the content in roots, stems and leaves of Houttuynia cordata, and provide a scientific basis for the exploitation and utilization of its resources. Method:Chlorogenic acid and rutin were analyzed on a Eclipse XDB-C18 (4.6 mm×150 mm, 5 μm)column. The mobile phase of chlorogenic acid was acetonitrile-0.1% phosphoric acid (12:88), and the detection wavelength was set at 326 nm. The mobile phase of rutin was methanol-0.1% phosphoric acid (50:50), and the detection wavelength was set at 355 nm. The flow rate was 1.0 mL·min-1. Result:The linear response ranged within 9.8-68.6 μg for chlorogenic acid (r=0.9997), 9.6-67.2 μg for rutin (r=0.9985). Recoveries were 99.2% with RSD 1.47% for chlorogenic acid, 100.1% with RSD 2.12% for rutin. Conclusion:There was a significant difference in the content of chlorogenic acid and rutin among the different parts of H. cordata. The content of chlorogenic acid and rutin in the sample from leaves was the highest than those from other parts, and it was lower in roots relatively.关键词：HPLC;Houttuynia cordata;different parts;chlorogenic acid;rutin
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC method of determining the content of hesperidin in Fructus Citris Sarcodactylis from different provenances. Method:An ultimate XB-C18 column was used. The mobile phase was acetonitrile and 2%acetic acid solution(18:82).The flow rate was 1.0 mL·min-1 with the column temperature of 30℃.The detection wavelength was set at 284 nm. Result:The linear range of hesperidin was 2.24-44.8 μg·mL-1 (r=0.999 9).The average recovery was 98.55%,and RSD was 0.97%(n=6). Conclusion:This method is simple, accurate,and can be used to evaluate the quality of Fructus Citris Sarcodactylis.关键词：Fructus Citris Sarcodactylis;hesperidin;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for simultaneous determining five avtive compounds from the whole plant of I. chinensis by HPLC. Method:The separation by gradient elution was performed on Hypersil ODS-2 column (4.6 mm×300 mm, 5 μm) at 30℃ with acetonitrile and water as the mobile phase and detection at 238 nm and 326 nm. Result:Five active compounds were linear in the detection range (r=0.999 3). The average recoveries of five active compounds were 97.08% (RSD 1.28%), 96.33% (RSD 1.64%), 96.50% (RSD 1.45%), 95.50% (RSD 1.38%), 96.00% (RSD 1.22%). Conclusion:The results indicated that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for I. chinensis.关键词：HPLC;simultaneous determination;Ixeris chinensis;five avtive compouds
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发布时间：2024-01-04 - 摘要：Objective:To establish a method to classify its quality and research for fingerprints of red peony root samples. R factor cluster analysis method and ANN are adopted in the study. Method:Collecting 40 red peony root samples from different places and classifying according to the chromatographic fingerprints of HPLC and traditional experience, the research has received discriminant functions by systematic cluster analysis with R factor cluster analysis method and ANN. Result:The similarity between two methods was 91.4%, there was difference between the samples of No.19, 27, 34. Conclusion:Two methods verified each other, a good method of assessing quality of the red peony root was established.关键词：Red Peony Root;chromatorraphic fingerprints;R factor cluster analysis;ANN
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC method for the content determination of pseudolaric acid B, benzoic acid and salicylic acid in Fufang Tujingpiding. Method:The analysis was performed on the Agilent Zorbax SB C18(4.6 mm×150 mm,5 μm) column with the methol-0.09% formic acid as the mobile phase at 25℃. The flow rate was 1.0 mL·min-1, and the detection wavelength was set at 260 nm for pseudolaric acid B, 230 nm for benzoic acid and salicylic acid. Result:The calibration curves were linear in the ranges of 1.29-25.78 mg·L-1(r=0.999 8) for pseudolaric acid B, 0.022-0.266 g·L-1(r=0.9999) for benzoic acid and 0.011-0.110 g·L-1(r=0.999 8)for salicylic acid. The average recoveries (n=6) of pseudolaric acid B,benzoic acid and salicylic acid were 100.4%(RSD 1.00%), 100.9% (RSD 0.84%) and 100.5% (RSD 0.75%), respectively. Conclusion:The developed method is accurate, convenient with good reproducibility that can be applied to the quality control of Fufang Tujingpiding.关键词：Fufang Tujingpiding;pseudolaric acid B;benzoic acid;salicylic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the assay of dihydroartemisinin from Dihydroartemisinin Soft Capsules. Method:The assay was performed by a Diamonsil C18 columm(4.6 mm×150 mm,5 μm) and a mobile phase of 0.1 mol·L-1 potassium dihydrogen phosphate(pH 2.5)-acetonitrile with radio of 65:35, and the flow rate was 0.8 mL·min-1.The wavelength of detector was set at 234 nm and the column temperature was at 18℃. Result:Dihydroartemisinin has a good linearity in the range of 8-40 μg (r=0.999 92,n=5),and the average recovery is 99.6% with RSD 0.7%(n=9). Conclusion:The method is simple, rapid and accurate and suitable for the determination of dihydroartemisinin in Dihydroartemisinin Soft Capsules.关键词：Dihydroartemisinin Soft Capsules;HPLC;dihydroartemisinin
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发布时间：2024-01-04 - 摘要：Objective:To develop an HPLC method for determination of narigin,hesperidin and neohesperidin in Muxiang Fenqi Pill. Method:The analysis was carried out with an Agilent C18(4.6 mm×250 mm,5μm)column and the mobile phase of acetonitrile-1% phosphoric acid (21:79).The flow rate was 1.0 mL·min-1 and column temperature was 30℃.The detection wavelength was set at 283 nm. Result:The calibration curve was linear within the range of 0.110 8-1.218 8 μg(r=0.999 4,n=6),0.226 8-2.494 8 μg(r=0.999 7,n=6)and 0.084 8-0.932 8 μg(r=0.999 4,n=6) for narigin, hesperidin and neohesperidin.The average recoveries were 97.5%(RSD 0.80%,n=6), 99.0% (RSD 1.7%,n=6) and 98.2%(RSD 1.6%,n=6) respectively. Conclusion:The results showed this method is reproducible, sensitive and accurate for determination of narigin,hesperidin and neohesperidin in Muxiang Fenqi Pill.关键词：Muxiang Fenqi Pill;narigin;hesperidin;neohesperidin;HPLC
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发布时间：2024-01-04 - 摘要：Objective:Detection of polysaccharide established on Panax notoginseng, determination of polysaccharide content in stem and leaf, ribs, flowers and rhizoma to provide the basis for quality evaluation on P. notoginseng. Method:The content of polysaccharides was determined by anthrone sulfuric acid method and detected at 528 nm by Shimadzu uv-2450. Result:There were significant defferences of the content of the extracted polysaccharides among P. notoginseng from different parts. Conclusion:Used method for the assay is simple, rapid, accurate, and can be used in quality inspection and control in different parts of P. notoginseng polysaccharide; Stem and leaf, ribs, flowers and rhizoma have some development value of polysaccharide.关键词：Panax notoginseng;polysaccharide;UV;different parts
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发布时间：2024-01-04 - 摘要：Objective:To verify whether the micro ribonucleic acid (miRNA) in Salvia miltiorrhiza and Rehmannia glutinosa, and their miRNA could enter human body blood through the digestive tract after oral adninistration. Method:At room temperature of 25℃, based on published miRNA sequences of S. miltiorrhiza (4) and R. glutinosa (6), 10 DIG-labeled antisense probes were synthesized. The total RNAs from S. miltiorrhiza and R. glutinosa were extracted with Plant Micro RNA extraction kit, and the total RNAs from human blood were extracted with blood miRNA extraction kit, followed the steps provided by the kit, and then loaded onto 15% denaturing polyacrylamide gel and hybridized with the appropriate DIG-labeled probes. Result:Northern blotting detected miR156 and miR157 in S. miltiorrhiza, and all the 6 miRNAs of miR5140,miR5139,miR 5138,miR 5137,miR 5142 and miR 5141 in R. glutinosa, and 3 miRNAs(miR5140, miR5137 and miR5141) in the test group which tested with R. glutinosa. Conclusion:These results present the evidence of miRNA in S. miltiorrhiza and R. glutinosa, and the miRNAs of miR5140, miR5137 and miR5141 from R. glutinosa could enter human body blood through the digestive tract after water frying. Further studies on the functions of these miRNAs may offer new insights and may lead to novel approaches to the development of Chinese traditional medicine.关键词：Salvia miltiorrhiza;Rehmannia glutinosa;miRNA;Northern blot
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发布时间：2024-01-04 - 摘要：Objective:To find out the best isolation conditions of protoplasts of Lonicera japonica. Method:Some influence factors on isolation of protoplasts, such as composition of enzyme solution, methods, hours and temperature of enzyme dissolving, osmotic pressure, and so on, were compared and analyzed to find out the best conditions of protoplasts isolation of L. japonica. Result:The callus flourishing well of L. japonica was used as materials for protoplasts isolation. 0.7 mol·L-1 mannitol+1.5% cellulose Onozuka R-10+0.25% pectolase Yakult Y-23 was the best composition of enzyme solution, which was dissolved in cell protoplast wash medium(CPW) solution. 12-hour enzyme dissolving at 25℃ was the best methods of protoplasts isolation. And standing was better than shaking. Conclusion:Abundant and superior protoplasts of L. japonica can be obtained under the best isolation conditions that this study has found out which will give the necessary help for protoplasts culture, cell fusion and genetic transformation of L. japonica.关键词：Lonicera japonica;callus;enzyme dissolving;protoplasts isolation
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发布时间：2024-01-04 - 摘要：Objective:Identifying the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti,to establish the quality of specificity evaluation index and Chinese medicine's active components alignment, which laid the foundation of new drug development. Method:The preparation of polysaccharides was extracted by the methods of water extracting-alcohol precipitating, then Sevag method sinking in protein, H2O2 bleaching and the use of dialysis method refined. The derivative change of the refined polysaccharide was analyzes by GC-MS. Result:The polysaccharide of Herba Lysimachiae Foenum-graeti is composed of rhamnitol, arabinose, ribose, glucose, and xylose, the proportion of it is 1.03:9.17:4.74:18.95:17.11. Conclusion:GC-MS could rapidly ascertain the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti. The water-soluble polysaccharide of Herba Lysimachiae Foenum-graeti can be used as a component of the quality evaluation index and drug components of the new drug research based material.关键词：Herba Lysimachiae Foenum-graeti;water-soluble polysaccharide;active components alignment;GC-MS;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the release characteristics of compound berberine hydrochloride colon specific tablets in rats. Method:The rats were administrated orally with the colon-specific tablets of diameter 3 mm and berberine hydrochloride (BH) suspension respectively. After administration, the rat's plasma and gastrointestinal tissues were collected at different time points within 22 h. The berberine in biosamples was determined by HPLC, using palmatine as internal standard. Drug delivery index (DDI) was calculated. Result:BH in plasma and gastrointestinal tissues showed good linear relationship in the range of 0.02-0.40 mg·L-1 (r>0.998) and 0.05-10.0 mg·L-1 (r>0.999) respectively. The release of BH in tablets mainly distributed in rat's caecum and colon and showed an obvious lag time. The value of Cmaxand AUC0-22 h in rat's colon were 3.1 times and 3.6 times of BH suspension group respectively. The DDI of the tablets was 0.00, 0.23, 1.17, 2.36, 8.55 in the rats' gastric, proximal and distal small intestinal, caecal, colonic tissue, respectively. Conclusion:The compound berberine hydrochloride tablet is a carrier for targeting the drug to the colon.关键词：compound colon specific tablets;berberine hydrochloride;tissue distribution;drug delivery index
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effects and mechanism of anger stress on behavior and memory in the D-gal induced brain aging model rats. Method:Wistar rats were randomly divided into control group, anger model group, D-gal group, anger+D-gal group, and attack rats. The D-galgroup and anger+D-gal group were administered ip 1% D-gal physiological salinesolution(PSS) 10 mL·kg-1·d-1. The control group and anger model group were ip given the same volume of PSS for 6 weeks. From the third weeks, the anger model group and anger+D-gal group accepted the anger stimulation test for 4 weeks. Each rat in anger model group was placed into one cage and accepted the attack from the invade rat, the tail of invade rat was clamped by using haemostat packed with bandage, two rats bite each other 20 min per day. Morris water maze test was carried out in the 37 days. Six days later all rats were executed and the brain tissue was collected for measurement of superoxide dismutase(SOD), malondialdehyde(MDA), lipofuscin (LPF), meanwhile, pathological changes were observed on cerebral cortex and seahorse. Result:Compared with control group, the behavior and memory significantly decreased(P<0.05, P<0.01), the brain tissue SOD activity wassignificantly reduced (P<0.01) and MDA, LPF content were obviously increased (P<0.01) in the anger model group, D-gal group and anger+D-gal group. Furthermore, pathological study showed that the cerebral cortex and seahorsein in the anger model group, D-gal group and anger+D-gal group appeared pathological changes compared with control group, the pathological changes in anger+D-gal group were most obvious. Conclusion:The anger stress has the negatively effects on behavior and memory in the D-gal induced brain aging model rats.关键词：anger stress;D-gal;brain aging;behavior and memory
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发布时间：2024-01-04 - 摘要：Objective:To study the protective effect of n-butanol extraction from Bupleurum chinense on acute liver injury in mice induced by carbon tetrachloride(CCl4) and to have a preliminary study on the features of its chemical constituent. Method:Preventive administration of total saikosaponin from B. chinense (high dose, middle dose, low dose) on acute liver injury mice induced by CCl4 (ig) lasted for 7 d, then detect the serum AST and ALT activity and liver tissue MDA content and SOD activity in all groups (blank group, model group, control group and saikosaponins groups), meanwhile construct determination and analysis of HPLC chromatogram of n-butanol extraction of B. chinense were carried out. Result:Total saikosaponin which mainly contained saikosaponin a(SSa) and saikosaponin d(SSd) was the main constituent of n-butanol extraction of B. chinense and it could significantly decrease ALT and AST activity in serum(P<0.05)and meanwhile it could obviously enhance the activity of SOD and reduce the contents of MDA in liver(P<0.05). Conclusion:The n-butanol extraction of B.chinense has protective effects on acute liver injury induced by CCl4 in mice and its main constituent was total saikosaponin.关键词：n-butanol extraction;acute liver injury;CCl4;HPLC;saikosaponin
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发布时间：2024-01-04 - 摘要：Objective:To explore the effects of Kanggu Zengsheng Jiaonang(KGZSJN) on experimental osteoarthritis in rats. Method:Animal models of osteoarthritis were obtained in rats by intra-articular injection of 4% papain for three times. Fifty rats were divided into five groups as normal group,model group, and three KGZSJN treatment groups. The animals began receiving drug orally every day one week after the last injection of papain. Six weeks later, all the animals were sacrificed for analysis. Histological examinations were performed on the articular cartilage. In the same time, the activities of superoxide dismutase(SOD)and malondialdehyde(MDA)in synovium were measured, the leves of nitric oxide(NO), nitric oxide synthase(NOS), interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α)in serum were measured respectively. Result:KGZSJN could significantly reverse cartilage degeneration, and enhanced the activitiy of SOD in synovium, KGZSJN reduced the activity of MDA in synovium, and the levels of NO,NOS,IL-1β,TNF-α in serum. Conclusion:KGZSJN can provide some protection in a dose-dependent manner in this model. The mechanisms are related to the reduction of expression of MDA,NO,NOS,IL-1β,TNF-α,and enhance the activities of SOD.关键词：Kanggu Zengsheng Jiaonang;osteoarthritis;mechanism
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effects of Sanao decoction on allergic airway inflammation in asthma mouse and its component analysis. Method:Sixty BALB/c mice were divide into six groups:normal group, model group, Sanao decoction high, middle, low dose(7.2,3.6,1.8 g·kg-1), positive group(0.75 mg·kg-1), mice in each group (except those in the normal group) were sensitized with 0.2 mL sensitized solution (OH)3] during the period from 15 to 28 day, and given corresponding drugs 30 min later, mice were atomized with 5% OVA; mice in normal group were sensitized with physiological saline and were atomized with physiological saline . Samples ofblood were collected for counting numbers of eosinophilic granulocyte(EOS), and broncho alveolar laverge fluid(BALF)was colledted for counting inflammatory cells. UPLC-TOFMS was used to analyze its chemical compositions. Result:Sanao decoction could obviously reduce numbers of EOS (P<0.01,P<0.05), in blood and BALF(P<0.05,P<0.01) and 22 compounds in Sanao decoction could be identified. Conclusion:Sanao decoction can obviously inhibit allergic airway inflammation in asthma mouse, 22 compounds which are related to effect of Sanao decoction are belong to alkaloids,flavonoids,saponins.关键词：Sanao decoction;allergic airway inflammation;component analysis
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of the extract of Valeriana jatamansi Jones on the blood lipid and liver function in experimental hyperlipidemia rats. Method:All the rats, except for the normal group, were fed with high-fat diets for 25 days to establish hyperlipidemia model. Then lovastatin group was given lovastatin at the doses of 3 mg·kg-1 once a day, and V. jatamansi high, medium, and low group were respectively given the alcohol extract of V. jatamansi at the doses of 1.25,2.5,5 g·kg-1 equivalently to the crude V. jatamansi by intragastric administration once a day. After administration, every group was still fed with high-fat diets. Two weeks later, the serum level of total cholesterol(TC), triglycerides(TG), lipoproten cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), alanine aminotransferase(ALT), aspartate aminotransferase(AST) and liver TC, TG was detected. Result:the TC, TG, LDL-C levels in the serums and livers in model rats were higher than these in normal rats(P＜0.05, P＜0.01), and the serum levels of ALT and AST also increased significantly(P＜0.05). Both lovastatin and the alcohol extract of V. jatamansi showed significantly decreased TG level in serum and liver(P＜0.01), the TC level in liver(P＜0.01)and the ALT and AST in serum (P＜0.01). Conclusion:V. jatamansi can regulate blood lipids level, and protect liver in hyperlipidemia rats.关键词：Valeriana jatamansi;experimental hyperlipidemia rats;blood lipid;liver function
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发布时间：2024-01-04 - 摘要：Objective:To observe the influence of ‘catharsis large intestine' on matrix metalloproteinase 9(MMP-9), transforming growth factor-β(TGF-β), Smad3 mRNA and Smad7 mRNA from rats lung with chronic obstructive pulmonary disease(COPD), and to investigate the mechanism of ‘treating from intestine'. Method:The rat model of COPD was established by cigarette smoking combined with intratracheal injection of lipopolysaccharide(LPS). All rats were randomly divided into normal control group, model group, treating intestine group, treating lung group, treating lung and intestine group. The normal control group, model group were intragastrically given the 10 mL·kg-1pure water solution, and other groups were intragastrically given corresponding herbal drugs (the charge for treating intestine group, treating lung group and treating lung and intestine group were 1.50,4.25,5.75 g·kg-1) for 14 days. The positive expression area ratio of MMP-9 and TGF-β were measured by immunohistochemical method, and the content of Smad3 mRNA and Smad7 mRNA were by Real time fluorescent quantitative PCR method from all groups of 40 rat's lung. Result:The model group of protein positive area ratios of MMP-9 and TGF-β became 1.231% and 3.002%, and the expression value of Smad3 mRNA was 14.804, and Smad7 mRNA was 0.2.The levels of MMP-9, TGF-β and Smad3 expression were markedly higher, while Smad7 expression were markedly lower, in the model group compared with those in the normal control group (P＜0.01). The levels of MMP-9, TGF-β became 0.944% and 1.989%, both of them and Smad3 were all lower in the treating intestine group than those in model group,while Smad7 levels keep higher(P＜0.05 or P＜0.01). The expression of TGF-β, Smad3 was lower but Smad7 expression grows up to 0.784 higher in the lung and intestine group than those in the lung group(P＜0.01). Conclusion:‘Ceatharsislarge intestine' can have effects on airway remodeling of lung in rats with COPD.关键词：chronic obstructive pulmonary disease;Xuanbai Chengqi decoction;treatment based on intestine;airway remodeling
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of Tripterygium granule on expression of bone marrow CD4+/CD8+T-lymphocytes in mice aplastic anemia model. Method:A mouse model of aplastic anemia was established and divided into five groups, normal group and model group (fed with normal saline), compound Tripterygium granule group (10.63, 13.29 g·kg-1, ig), testosterone undecanoate group (22 mg·kg-1, ig). After continuous administration of 35 d, bone marrow mononuclear cells were separated and cultured for 24 h. Expression levels of CD4+, CD8+, CD4+/CD8+T cells were analyzed by flow cytometry. Result:Compared with the normal group, amount of CD4+, CD8+, CD4+/CD8+T cells was decreased in model group, which of CD4+, CD4+/CD8+T cells were significantly lower than the normal group (P<0.05), CD4+and CD8+were decreased, while CD4+/CD8+was increased in testosterone undecanoate group, but there was no significant difference compared with model group. Compared with the model group, amount of CD4+, CD8+, CD4+/CD8+in high and low dose group of compound Tripterygium granule was increased, which of CD4+, CD4+/CD8+T cells was significantly different from model group and testosterone undecanoate group (P<0.05). Conclusion:Compound Tripterygium granule can regulate the abnormal state of bone marrow T cells, restore balance between T cells and T cell subsets, and promote the recovery of bone marrow function.关键词：aplastic anemia;compound Tripterygium granule;testosterone undecanoate;T cell subsets
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effects of Huoxiang Zhengqi liquid on the expression of AQP4 in colon mucosa in rats with dampness retention syndrome (DRS). Method:Twenty four SD rats were randomly divided into 3 groups:control group,DRS group and treatment groups (DRS+Huoxiang Zhengqi liquid), 8 rats in each group.DRS was induced in all rats,except the control group,by modified environmental and fatigued method for 6 days.After DRS model was copied,control and DRS groups were orally given with normal saline(NS, 20 mL·kg-1). Rats in treatment groups were orally given with Huoxiang Zhengqi liquid(20 mL·kg-1) for 8 days.Then the expression of aquaporin 4(AQP4) on colon mucosa was determined by immunohistochemistry and Real-Time PCR. Result:Rats in model groups exhibited typical DRS with sign of hyperaemia, edema, hemorrhagic spots and bleeding in the gastric mucosa compared to control. The expression of AQP4 in colon musoca significantly decreased in DRS group compared to control group(P<0.05).Conversely, exposure of DRS rats to Huoxiang Zhengqi liquid resulted in increase in expression in AQP4 in the colon mucosa(P<0.05 versus DRS group). Conclusion:Huoxiang Zhengqi liquid could have the effect of curing the DRS-induced diarrhoea, increased expression of AQP4 may play an important role.关键词：Huoxiang Zhengqi liquid;dampness retention syndrome;colon mucosa;AQP4
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of N-acetylcysteine (NAC) on acute injured kidney (AKI) induced by cisplatin (CDDP). Method:The rats were injected CDDP intravenously to make AKI model. The rats were randomly divided into 6 groups, including the normal control group, CDDP-induced AKI model group, NAC groups (50, 100,200 mg·kg-1) and Vit C control group (100 mg·kg-1). NAC and Vit C were administered once a day three days before CDDP was given. And then NAC and Vit C were continuously given ip for five days. The body weight, kidney weight, kidney index, urinary protein excretion rate of rats were determined. The commercial kits were employed to test the concentrations of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT),reduced glutathione (GSH), glutathione peroxidase (GSH-Px), 15-F2t-isoprostane (15-F2t-Isop). Eight-OHdG positive cells were counted by immunohistochemical staining. The expression of Cu-Zn-SOD was measured by Western blot. Result:Compared with the normal control group, the weight of body and kidney, kidney index, the concentration of CAT, SOD, GSH, GSH-Px and Cu-Zn-SOD expression were significantly decreased, while urinary protein excretion rate and the concentration of LDH, MDA, 15-F2t-Isop, 8-OHdG positive cells were significantly increased (P<0.01) in the CDDP-induced kidney injury group. NAC and Vit C significantly reduced urinary protein excretion rate and the concentration of LDH, MDA, 15-F2t-Isop, 8-OHdG positive cells and increased kidney index, the concentration of CAT, SOD, GSH, GSH-Px and Cu-Zn-SOD expression compared with the AKI model group (P<0.01). Conclusion:NAC protect kidney from CDDP injury by suppressing the oxidative stress.关键词：N-acetylcysteine;cisplatin;acute kidney injury;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective:To observe the antioxidation and anti-inflammation effect of genistein (Gen) on rat myocardial hypertrophy caused by isopropylarterenol (Iso). Method:The rat myocardial hypertrophy model was reproduced through hypodermic injection which lasted 10 days at back skin with Iso, 1 mg·kg-1·d-1. Once the model was succesfully made, rats were administrated with corresponding doses of Gen, DMSO or normal saline through hypodermic injection from the second day, and the process lasted 14 days. After the last administration, the rats were lasted overnight and samples of blood and heart were collected under anesthesia status. The indexes of left ventricle were calculated, the activity of superoxide dismutase(SOD), myeloperoxidase(MPO) and the concentration of malondialdehyde(MDA) in serum were detected, and interleukin-2(IL-2) in myocardial tissue was investigated with radioimmunity kits. Result:In model group, there was a marked increase in the left ventricle index, the activity of SOD in the serum was markedly decreased, the content of MDA and the activity of MPO in the serum were increased, the level of IL-2 in myocardial tissue of rats was increased. Treatment with Gen significantly increased the activity of SOD in the serum, decreased the content of MDA and the activity of MPO in the serum, reduced the level of IL-2 in myocardial tissue. Conclusion:Gen has antioxidation and anti-inflammation effect on the myocardial hypertrophy caused by Iso.
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发布时间：2024-01-04 - 摘要：Objective:To study the anti-tumor effect of neo-gambogic acid(NGA)on Hep G2 xenograft in nude mice in vivo and its mechanism. Method:Sixty healthy BALB/c-nu mice, transplanted by Hep G2 hepatocarcinoma cells, were divided into 5 groups, including model group, 5-FU(10.0 mg·kg-1) group and three groups of neo-gambogic acid in high dose, middle dose and low dose(8.0, 4.0, 2.0 mg·kg-1). The mice were executed after fourteen days of treatment every other day (qod) and tumor tissues were excised. The size of tumor tissues was measured and tumor inhibition rate was calculated. Immunohistochemistry was used to detect the expressions of Bax, Bcl-2, p-ERK1/2 and p-MEK1/2. Result:the tumor weight of NGA groups was reduced remarkably compared with control group. The tumor inhibition rate of NGA high-dose (8.0 mg·kg-1) group was 83.75%, which excelled the tumor inhibition rate of the 5-FU (10.0 mg·kg-1) group (46.54%, P＜0.05). The expression of Bax was up-regulated by NGA dose dependently while the expression of Bcl-2 was down-regulated. The expressions of p-ERKl/2 and p-MEKl/2 in NGA groups were reduced remarkably compared with control group (P＜0.05). Conclusion:NGA exerts exact anti-tumor effect on Hep G2 xenograft in nude mice in vivo. Its mechanism correlates with that the cells in Hep G2 xenograft are induced apoptosis via up-regulating the Bax/Bcl-2 ratio and that the phosphorylation level of mitogen-activated protein kinase (MAPK) signal transduction pathway is down-regulated.关键词：neo-gambogic acid;anti-tumor;nude mice;apoptosis;MAPK
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发布时间：2024-01-04 - 摘要：Objective:To elplore the effects of the component compatibility of panax notoginseng saponinsn and icariin on promoting the proliferation and calcification of osteoblasts. Method:The third-fifth generation cells were as experimental model, osteoblast density was 1×105/mL,the concentration of Panax notoginseng saponins and icariin was 1,10,20,50 mg·L-1,MTT was applied to determine proliferation of the cell and calcification. Alizarin red staining method was used to observe calcification. Result:Panax notoginseng saponins and icariin in a dose of 10,20,50 mg·L-1 were able to significantly promote the proliferation of osteoblasts. When the component compatibility of panax notoginseng saponinsn and icariin was 5:2, compared with those of control group, the counts of calcified nodules in the group was significantly higher. The component group can promote proliferation of osteoblasts. Conclusion:The component compatibility of panax notoginseng saponinsn and icariin is feasible from the proliferation and calcification of osteoblasts.关键词：panax notoginseng saponins;icariin;component formula;Osteoblast;proliferation;calcification
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发布时间：2024-01-04 - 摘要：Objective:To investigate effects of Buyang Huanwu decoction containing serum on the morphology and viability of PC12 cells during oxygen-glucose deprivation. Method:Buyang Huanwu decoction containing serum was obtained by the seropharmacological method. PC12 cells were treated with different concentrations (5%, 10%, 20%) of the serum during oxygen-glucose deprivation. The cell morphology was observed with inverted microscopy, and the cell viability was examined by methyl thiazolyl tetrazolium assay. Result:The cell morphology was changed and the cell viability was decreased during oxygen-glucose deprivation, and the injured degree of cell morphology was relieved and the cell viability was increased in PC12 cells which treated with concentrations of 5%, 10% Buyang Huanwu decoction medicated serum for 12, 18, 24 h(P<0.01). However,20% Buyang Huanwu decoction containing serum had the bidirectional regulation effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation. Conclusion:The certain concentration of Buyang Huanwu decoction congtaining serum has protective effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.关键词：Buyang Huanwu Decoction;medicated serum;oxygen-glucose deprivation;PC12 cells
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of ethanol extracts from Euphorbia humifusa on monilial vaginitis in rats. Method:After the candidal vaginitis model establishment, ethanol extracts from E. humifusa was intragastrically administered daily for 7 days.Candida albicans of the vaginal exudates were cultured and analyzed. The levels of MCP-1and MIP-2 in vaginal lavage fluid were detected by enzyme-linked immunosorbent assay(ELISA)method. The pathological changes of vaginal tissue were examined under light microscope. Result:E. hmuifusa ethanol extracts inhibited the C. albicans significantly (P<0.001) and decreased the MCP-1and MIP-2 levels in vaginal lavage fluid remarkably. In addition, the ethanol extracts ameliorated the vaginal pathological changes significantly (P<0.001). Conclusion: E. humifusa ethanol extracts has remarkable antifungal activity by inhibiting the C. albicans.关键词：Euphorbia humifusa ethanol extracts;Candida albicans;monilial vaginitis;chemokines
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of the Liuyueqing polysaccharide against CCl4-induced liver fibrosis in rats and its mechanism. Method:SD rats were divided into two groups randomly:hepatic fibrosis model group and normal group. The model group of rats with hepatic fibrosis was established by 1 mL·kg-1 of 50% CCl4,ig, twice per week for continuous 7 weeks. The rats in the normal control group were given normal saline (NS). Sixty SD rats with hepatic fibrosis confirmed by the pathological inspection was randomly divided into LYQP(600,300,150 mg·kg-1) groups, colchicine(0.2 mg·kg-1) group and model group. All rats were treated with drugs or NS for four consecutive weeks by ig, once a day. The activity of alanine aminotransferase(ALT), aspartate aminotransferase(AST), and glutathione synthesis(GSH-Px) in serum of rats and the content of hydroxyproline (Hyp) in hepatic tissue were determined. The extent of liver fibrosis was observed by Masson staining. Result:LYQP significantly decreased the activity of ALT and AST in rats with liver fibrosis. Compared with model control group, the content of Hyp in hepatic tissue was decreased significantly. The activity of GSH-Px was significantly increased. Masson staining showed that the collagen fibers in every treated group were less than model control group. Conclusion:Liuyueqing polysaccharide can relieve liver fibrosis induced by CCl4.关键词：Liuyueqing;polysaccharide;carbon tetrachloride;liver fibrosis
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发布时间：2024-01-04 - 摘要：Objective:To observe the therapeutic effect of the Aikekang capsule on the infection of Friend leukemia virus in mice. Method:Seventy BALB/c mice were randomly divided into seven groups:normal group,model group,Zidovudine group(0.1 g·kg-1),Tangcaopian group(3.2 g·kg-1), Aikekang groups (5,2.5, 1.25 g·kg-1). Except for the normal group, the mice in other groups were inoculated intraperitoneally with Friend leukemia virus(FLV) to acquired immuno defiency syndrom (AIDS) model. Twenty-four hours after inoculation, the mice were given corresponding drugs according to the experimental design for 21 days. After treatment,the mice were executed and the effects of the Aikekang capsule on the spleen index, CD4+cells and CD8+cells, CD4+and CD8+T cells were determined. Result:High-dose and middle-dose Aikekang had reduced the spleen index and the percentage of CD4+memory T cell and rised the percentage of CD4+, the ratio of CD4+/CD8+, the percentage of CD4+immature T cell and the percentage of CD8+immature T cell; High-dose Aikekang increased the percentage of CD8+and reduced the percentage of CD8+memory T cell. Conclusion:The Aikekang capsule can enhance the immunity on the infection of Friend leukemia virus in mice.关键词：Aikekang capsula;Friend leukemia virus;AIDS
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发布时间：2024-01-04 - 摘要：Objective:To investigate the influence of L-theanine on amino acids neurotransmitter and GluRs in rats by acute exhaustion swimming and mechanism of L-theanine improves exercise capacity. Method:SD male rats were randomly divided into high, middle and low dose (400, 200, 100 mg·kg-1, ig) groups and normal control group, ig, (n=10, in each group). After one week, each group was started to give L-theanine for 30 days. The normal control group was given normal saline. On the day 31,rat exhaustive swimming time was measured. Meanwhile, Glu, Gly, GABA and Asp of corpus striatum in rats were determined by HPLC. Immunoprecipitation and immunoblotting were applied to analyze the level of NR2A and tyrosine phosphorylation. Result:Compared with the normal control group, swimming time in high and middle dose groups increased significantly . Level of Glu, NR2A, tyrosine phosphorylation showed obviously higher in high dose group than the normal control group·L-1,(134.1±3.6), (123.2±4.4), (P<0.05)]. High dose group also showed lower GABA(1 881.3±336.1)μmol·L-1 level than normal control group (2 237.1±198.4) μmol·L-1(P<0.05). Conclusion:L-theanine has the effect of improving exercise capacity in rats possibly through enhancing Glu, NMDAR2A, tyrosine phosphorylation level and inhibiting the synthesis or secretion of GABA in brain.关键词：L-theanine;exercise capacity;nerve center
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发布时间：2024-01-04 - 摘要：Objective:To investigate the antidepressant effect of EtoAc extracts of Gastrodia elata(EEG). Method:Male mice were randomly divided into control group,fluoxetine group(20 mg·kg-1),chlorimipramine group (20 mg·kg-1)and EEG low,high dose groups(0.063, 0.126 g·kg-1). Different groups were intragastrically administrated corresponding medicine once per day for seven days. Then tail suspension test and forced swimming test were used to observe immobility time of mice. And open field test were applied to observe autonomic activity of mice. Result:Intragastric administration of EEG significantly reduced immobility time in both forced swimming test(0.126, 0.063 g·kg-1) and tail suspension test (0.126 g·kg-1)in mice. However, EEG did not affect the number of autonomic activitt of mice in the open-field test. Conclusion:The EEG shows an antidepressant-like effect in the despair animal models of depression in mice.关键词：Gastrodia elata;antidepressants;forced swimming test;tail suspension test;open field test
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effects and mechanism of cycloastragenol (CAG) on aging. Method:Mouse aging model was induced by D-galactose. Sixty mice were randomly divided into control group, model group, CAG(2.5, 5.0, 10.0 mg·kg-1)group and vitarmine E(VE, 2.5 mg·kg-1)group.Each group was given ip D-galactose except normal control group was supplied with equal amount of physiological saline for 6 weeks, the treated mice were given medicines according dose. The activity of total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD), the contents of malondialdehyde (MDA) and hydroxyproline (HYP) were mwasured. Result:Compared with the control group, the activity of T-SOD,T-AOC and the content of HYP in liver, heart and skin were markedly reduced in model group (P<0.01),while the content of MDA was markedly increased (P<0.01, P<0.05);CAG group, compared with model group, significantly improved the activity of T-AOC in liver, heart and skin (P<0.01). Meanwhile, the activity of T-SOD in liver, heart and skin(P<0.01)and the content of HYP(P<0.01, P<0.05)were markedly increased. And it significantly reduced the content of MDA(P<0.01, P<0.05). Conclusions:CAG has a remarkable effect of anti-decrepitude, the possible mechanism is able to improve the activities of T-SOD, T-AOC and reduce the contents of MDA, HYP.关键词：cycloastragenol;anti-aging;T-AOC;T-SOD;MDA;HYP
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发布时间：2024-01-04 - 摘要：Objective:To investigate anti-atherosclerosis effect in rats of the extracts of Radix Salviae Miltiorrhizae(RSM), Fructus Crataegi(FC),as well as their interaction. Method:Male Wistar rats were randomly divided into 2 groups:normal group and model group. The atherosclerotic model was established by injection of VD3 and ovalbumin,while fed with high cholesterol diet. After the model was determined successfully,all model rats were divided into normal group,model group,RSM group,FC group,mixture of RSM and FC group. Each group was given the corresponding drug for 4 weeks. After the end of treatment,blood serum were analyzed for blood-fat levels,the contents of MDA, SOD and NO,observing ortic organization morphological changes by the method of(HE) dyeing. Result:The TC,TG,LDL-C and MDA level were reduced,while the HDL-C,the serum SOD,NO were raised in the drug intervention groups. And for many indexes, there was obvious (P<0.05) effect for the mixture of RSM and FC group. Conclusion:RSM and FC show synergistic effect,the combined effect is better.关键词：atherosclerosis;Radix Salviae Miltiorrhizae;Fructus Crataegi;synergy
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发布时间：2024-01-04 - 摘要：Objective:To explore the influence of Yisui Shengxue granule (YSG) on the kidney medullary loss in mice model induced by radiation damage. Method:Whole body irradiation of 60Coγ-ray was used to induce mice model of kidney medullary loss. The model showed bone marrow hematopoietic inhibition and reduction in visible components in peripheral blood. YSG(23.6,11.8,5.9 g·kg-1,ig) was preventively given 2 days before irradiation and successively given 14 days after irradiation. Peripheral blood indexes were examined before radiation and at the 1, 2, 5, 7, 9, 11, 13, 15, 17, 19 and 21 days after radiation. Result:In the positive drug(G-CSF) group, the WBC count was significantly increased after radiation at the 1-17 days compared with those in model group(P<0.05, P<0.01). The WBC count in three dosage groups of YSG at the 1, 5, 7, 13 and 17 days after radiation was elevated(P<0.05 or P<0.01). The RBC count and hemoglobin were descended to a certain extent after irradiation.The RBC count in the group of positive drug(G-CSF) and all dose groups of YSG was higher than that in the model group(P<0.05 or P<0.01). Platelet count was decreased in the 5 day after radiation and reached the lowest level in the 7 day and then rose slowly in model group. However, the platelet count in the treatment group was rapidly recoveried from the 5 day after radiation, and those in the high dosage group were much higher than those in the model group in 5, 11 and 13 days, and that in the medium dosage group in the 13 day was higher than that in the models(P<0.05, P<0.01, P<0.001). Conclusion:All dose groups of YSG can increase the WBC count significantly after radiation damage and promote the RBC, hemoglobin and platelet count recovery. The results suggest that the recipes of promoting kidney and tonifying bone have the function of supplying the kidney essence and protecting marrow and also can promote hematopoietic.关键词：tonifying the kidney to benefit marrow;radiation damage;Yisui Shengxue granule;peripheral blood
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发布时间：2024-01-04 - 摘要：Objective:To analyze the relationship between toxicity and cardiotonic effect of aconitine, calculating the therapeutic index (TI) of aconitine. Method:Regarding rat's electrocardiogram (ECG) change as the index,the toxicity to heart induced by aconitine was studied. Nimodipine, one of Ca2+ antagonist,was used to make heart failure model in rats. The aconitine was injected through femoral vein continuously. The effect on heart rate,+dp/dtmax,LVDP-rate pressure product(RPP) was observed. Result:Regarding RPP or +dp/dtmax as the index of ED50 and regarding PVC or CA as the index of TD50 or LD50,LD50/ED50 or TD50/ED50 was used to calculate the therapeutic index (TI) of aconitine, TI(RPP)were 1.84,17.22 and TI(+dp/dtmax)were 1.23,11.54 respectively. Conclution:Aconitine has cardiotonic effect but has toxicity to heart. TI is small.
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of total saponins of astragalus (TSA) on ferroportin 1 in rats with anemia of ahronic disease (ACD) induced by complete Freund's adjuvant. Method:Complete Freund's adjuvant was intradermally injected into foot pad of rats to duplicate ACD model. Then TSA (different concentration, respectively 50, 16, 5 mg·kg-1) was given for 7 days by peritoneum. Erythropoietin was used as the positive control. Interleukin-6(IL-6)in serum was detected with (Enzyme linked immunoassay, ELISA) and the Fpn1 mRNA (in spleen, liver and duodenum) and Hamp mRNA (in liver) were detected with Quantitative real-time Polymerase Chain Reaction (qPCR). Result:IL-6 in serum, Fpn1 mRNA in liver and duodenum, Hamp mRNA in liver were increased obviously (P＜0.05), but there wasn't any change in Fpn1 mRNA in spleen. Compared with model group, IL-6 were decreased significantly both by high and middle dose (P＜0.05), but indistinctively by low dose. Fpn1 mRNA in liver and duodenum, Hamp mRNA in liver were decreased significantly and Fpn1 mRNA in spleen was increased in all TSA groups (P＜0.05). Otherwise, the effect of TSA on above-mentioned indexes was prior to positive drug (P＜0.05). Conclusion:There was obvious increasing of Fpn1 in liver and duodenum in ACD rats, but almost no change in spleen. Fpn1 decreasing corresponding to hepcidin increasing were not found in this experiment. TSA was found to have different effect to Fpn in different tissue.关键词：complete Freund's adjuvant;total saponins of astragalus;anemia;chronic disease;ferroportin;hepatic bactericidal protein
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发布时间：2024-01-04 - 摘要：Objective:To observe the protective effect of ligustrazine and ferulate on myocardial ischemia-reperfusion injury and probe into its possible molecular mechanism in rats. Method:Sixty male rats were randomly divided into five groups:sham-operation group,ischemia-reperfusion group,ligustrazine(4 mg·kg-1) group,ligustrazine+ferulate low dose(4 mg·kg-1) group,ligustrazine+ferulate high dose(8 mg·kg-1) group.The rat model with ischemia-reperfusion injury was established with 30 min of myocardial ischemia and followed by 120 min reperfusion.The rats were treated for 10 min before reperfusion through jugular vein injection.After the reperfusion was finished, the biochemical indicators in serum and histological index in myocardium were investigated. Result:Compared with ischemia-reperfusion group,ligustrazine and ferulate could lower the level of creatine kinase isoenzyme(CK-MB),lactate dehydrogenas(LDH),cardiac troponin I(cTnI) and malondialdehyde(MDA) in blood,increase the activity of total-superoside dismutase(T-SOD) in blood,reduce the myocardial infarction size,increase the expression of Bcl-2 protein,decrease the expression of Bax protein,reduce the myocardial apoptosis index and Bcl-2/Bax ratio(P＜0.01);all indicators for ligustrazine and ferulate showed dose-dependently superior to ligustrazine(P＜0.05或P＜0.01). Conclusion:Ligustrazine and ferulate had a well protective effect on rats with myocardial ischemia-reperfusion injury.Anti-apoptotic effect was related to up-regulate the expression of Bcl-2 and down-regulate the expression of Bax by ligustrazine and ferulate.关键词：ligustrazine ferulate;myocardial ischemia-reperfusion injury;apoptosis;Bcl-2 gene;Bax gene
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发布时间：2024-01-04 - 摘要：Objective:To investigate the mechanism involved in anticonvulsant effect of gentianine in rats with recurrent febrile seizures. Method:Sixty SD rats were randomly divided into the healthy control group, model group, gentianine high-dose (200 mg·kg-1) group, gentianine low-dose (50 mg·kg-1) group (n=15, each). Except the health control group, all rats were induced high fever seizures according to the reference method. The rats were induced convulsion every other day, totally 10 times in 20 d. When convulsion happened,gentianine was injected (ip) immediately in the gentianine high-dose group and the low-dose group. In model group and health control group,the rats were given the same volume of normal saline. Convulsion latency and convulsion duration; serum IL-1, IL-6, IL-8 and TNF-α, IFN-α levels; brain homogenate cAMP and PGE2 level were investigated for the rats. Result:In gentianine groups, convulsions incubation period was significantly longer than that in the model group (P<0.05); convulsion duration was significantly less than that in the model group (P<0.01). In model group, IL-1, IL-6, IL-8, TNF-α and PGE2 levels were significantly higher than those in the control group and gentianine groups (P<0.05). In gentianine high-dose group, IL-1, IL-6, IL-8, TNF-α and PGE2 levels were significantly lower than those in gentianine low-dose group (P<0.05). Conclusion:Gentianine has antipyretic and anticonvulsant effect, which has relationship with cytokines affecting the hypothalamic PGE2 levels.关键词：gentianine;convulsions;mechanism
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发布时间：2024-01-04 - 摘要：Objective:To investigate the suppressive effect of gypenosides(Gyp) on human hepatocellular carcinoma Bel-7402 cells. Method:The growth inhibitation of human hepatocellular carcinoma Bel-7402 cells was detected by MTT assay. The DNA damage of human hepatocellular carcinoma Bel-7402 cells was detected by single cell gel electrophoresis; RT-PCR was utilized to investigate the apoptosis of human hepatocellular carcinoma Bel-7402 cells and its relationship with caspase-8 from molecule level. Result:Gyp (100-400 μg·mL-1) inhibied the growth of human hepatocellular carcinoma Bel-7402 cells. DNA damage was detected after Gyp (182 μg·mL-1) treated for 4,8,12,16,24 h. The change reached maximum at 12 h(81.15±14.23)% and with time-dependent. caspase-8 expressed strongly in Gyp group and nothing in control. Conclusion:Gyp has effects on cell viability,it could induce human hepatocellular carcinoma Bel-7402 cells apoptosis,DNA damage and caspase-8 expression.关键词：gypenosides;human hepatocellular carcinoma Bel-7402 cells;proliferation and apoptosis
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发布时间：2024-01-04 - 摘要：Objective:To study the antiallergic action of Shuningkang by investigating the effect of aqueous extract from Shuningkang on mast cell degranulation and histamin releasing in vitro by PMC degranulation percentage and histamine releasing assay. Method:OVA was used as allergen. In the concentration of 40 mg·L-1,30 min conditions, the influences of aqueous extract from Shuningkang 0.1,1,10 g·L-1on sensitized rat mast cell degranulation and histamine release were investigated. Result:The extract of Shuningkang could inhibit rat PMC degranulation and histamine releasing in vitro. PMC degranulation rates in model group,high, middle, low does groups of Shuningkang and Cromoglicate Sodium group were (47.53±5.57)%,(25.11±2.56)%,(35.71±6.99)%,(47.11±4.26)% and(22.87±6.52)% respectively. Shuningkang showed significant inhibition on histamine release, compared with the model group. In the high, middle, low does groups of Shuningkang and Cromoglicate Sodium group, the inhibition rates on histamine release were 16.85%,20.06%,6.58%,16.85%. Conclusion:Shuningkang has certain effect on skin allergies.关键词：Shuningkang;mast cell degranulation;histamine release
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发布时间：2024-01-04 - 摘要：Objective:To study the growth-inhibiting effect of polyprenols from Ginkgo biloba on breast cancer cells MCF-7 in vitro and the related molecular mechanism. Method:Polyprenols were extracted by preparative Reversed-phase high-performance liguid chromatography(RP-HPLC). The growth-inhibiting effect of polyprenols from Ginkgo biloba on breast cancer cells MCF-7 was evaluated in vitro. The polyprenols with best antiproliferative effect was screened and figured out. The influence of polyprenol P3 (5,10,20 mg·L-1) on mitotic cycle and apoptosis of MCF-7 cells was analyzed with flow cytometry. Result:Ten polyprenol monomers were obtained by preparative RP-HPLC, and were orderly named P1, P2, P3……P10.The best effective growth-inhibiting on MCF-7 cells was P3 with IC50 of 10.32 mg·L-1. At low concentration,its mechanism appeared to be delaying on G2/M of mitotic cycle, and at high concentration to be inducing cell apoptosis. Conclusion:Polyprenol P3 from Ginkgo biloba could inhibit growth of breast cancer MCF-7 cells in vitro. Its molecular mechanism is linked with delaying on mitotic phase (G2/M) and inducing cell apoptosis.关键词：Ginkgo biloba leaves;polyprenols;RP-HPLC;MCF-7 cells;mitotic cycle.
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发布时间：2024-01-04 - 摘要：Objective:To explore effective way to optimize the proportion of effective components from Panax notoginseng and Crataegus pinnatifida Bge. Method:According to table U6(64) in uniform design, mice were divided into 6 treatment groups, the ratios between flavonoids of Crataeguspinnatifida and total saponins of P. notoginseng were 200:15,100:1.875,50:30,25:3.75,12.5:60,6.25:7.5(mg·kg-1)accordingly. The treatments lasted for 5 days. Then, survival time of mice were evaluated by two models, isoproterenol injection intraperitoneally and acute cerebral ischemia induced by broken neck. The best proportion of effective components was calculated by multiple regression equations. Result:The best ratio appeared to be 20:15 mg·kg-1,namely, the ratio of 4:3.The proportion could prolong the survival time of mice with acute cerebral ischemia (P＜0.05). Conclusion:The combination of uniform design and animal models in pharmacodynamics is an effective way to optimize the proportion of Chinese medicinal components.关键词：Panax notoginseng;Crataeguspinnatifida;combination of effective components;uniform design
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发布时间：2024-01-04 - 摘要：Objective:To establish a rat model of gastric ulcer by chronic psychological stress, observation the effects of Jiawei Sini San (JWSNS) on gastric mucosal morphology, plasma adrenocorticotropic hormone (ACTH), serum cortisol (CORT) and blood flow in gastric mucosal of model rats, and study the mechanism of protection. Method:Wistar rats were randomly divided into 5 groups:normal group, model group, JWSNS small, middle, big doses (10, 5, 2.5 g·kg-1) groups, omeprazole (3.0 mg·kg-1) group. Besides normal group, gastric ulcer was induced by chronic psychological stress in other groups using 10 unpredictable stimulation methods in four weeks. After two weeks of the stress, the treatment groups were ig given the drugs (in 2 mL of volume) accordingly, while the blank control group and model group were gavaged with normal saline of 2 mL daily After 4 weeks, morphological changes in gastric tissue were observed, gastric mucosa ulcer index (UI),gastric mucosal blood flow (GMBF), plasma ACTH and serum CORT were determined. Result:After 4 weeks, gastric mucosa in model group showed diffuse bleeding ulcer. Compared with the normal group, the gastric mucosa injury was obvious, UI, plasma ACTH, serum CORT were significantly higher (P<0.01), while GMBF, gastric mucosa repair rate were significantly decreased (P<0.01). Compared with the model group, JWSNS dose groups showed reduced gastric mucosal injury, the gastric mucosal UI, plasma ACTH, serum CORT were significantly decreased (P<0.01 or P<0.05), while GMBF, gastric mucosa repair rate were increased significantly (P<0.01 or P<0.05). Conclusion:Chronic psychological stress could damage gastric mucosa significantly in rats, inducer gastric mucosal UI, elevate plasma ACTH and serum CORT, decrease GMBFand gastric mucosa repair ability. JWSNS could reduce significantly psychological stress-induced gastric mucosal injury, effectively reduce the ulcer index, regulate neuroendocrine function, increase GMBF and improve mucosal repair capacity.关键词：Jiawei Sini San;psychological stress;gastric mucosa blood flow;mucosal ulcer index;adrenocorticotropic hormone;cortisol
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of Kangxin decoction on regulating vitamin D in rats with myocardial hypertrophy induced by isoproterenol. Method:Fifty male SD rats were randomly divided into 5 groups:normal group, model group, three dose groups of Kangxin decoction (10, 5, 2.5 g·kg-1). Myocardial hypertrophy model in rats was induced by subcutaneous injection of isoproterenol (ISO, 2.4 mg·kg-1, 7 d). After Kangxin Decoction intervention for 6 weeks, serum 25(OH) D3, atrial natriuretic peptide (ANP), renin were detected by ELISA. Pathological changes in myocardial tissue were determined by hematoxylin-eosin (HE) staining. Result:Compared with the controls, serum 25(OH) D3 in the model group was reduced, and serum ANP and renin were elevated (P<0.05).Myocardium derangement and fibrotic hyperplasia were also seen in the models. After Kangxin decoction treatment, serum 25(OH) D3 was increased, accompanied with lower ANP and rennin. The degree of myocardial fibrosis was improved after the treatment. Conclusion:Kangxin decoction had an effective role to treat myocardial hypertrophy induced by ISO by regulationg vitamin D and protecting myocardial cells from injury.关键词：myocardial hypertrophy;vitamin D;renin;atrial natriuretic peptide;Kangxin decoction
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发布时间：2024-01-04 - 摘要：Objective:To study Disporum sessile's effects of anti-cough and anti-sputum. Method:Mice were randomly divided into 6 groups:blank, positive medicine, ethyl acetate layer high, low doses (2.5,1.25 g·kg-1) and water layer high, low doses (2.5,1.25 g·kg-1). Drugs were ig administrated for 5 days. Cough caused by strong ammonia in mice was used to evaluate anti-cough effect. Phenol red applied to trachea in mice was used to observe the efficacy of anti-sputum. Trachea clips isolated from rabbit were randomly divided into groups:positive medicine, ethyl acetate layer low, medium, high doses (100,10,1.0 g·L-1) and water layer high, medium, low doses (100,10,1.0 g·L-1). The model of cilia movement was applied to observe the efficacy of promotion of cilia movement. Result:The ethyl acetate layer showed significant anti-cough effect(P＜0.01). The phenol red concentration of the water layer was higher than that of the ethyl acetate layerwith no significant difference. High concentration of the ethyl acetate layer and the water layer could obviously quicken the speed of the rabbit's cilia movement. Conclusion:The acetate layer and the water layer extract of Disporum sessile has the effects of anti-cough and anti-sputum.The herb's active ingredients of anti-cough effect mainly exists in ethyl acetate layer.
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of Zibu Piyin Recipe (ZBPYR)on spinal cord injury and the influence on expression of hypoxia inducible factor(HIF) -1α. Method:Thirty-six adult Wistar rats were divided into six groups randomly:sham operated group, positive control group(hydergin, 0.6 mg·kg-1), ZBPYR low, middle and high dose group(7,14,21 g·kg-1), SCI group. The rats were killed at 72 h post-injury. The expression of HIF-1α and HIF-1α mRNA was detected with RT-PCR and Western-blot assay. Result:At 72 h post-injury, the expression of HIF-1α and HIF-1α mRNA in SCI group were higher than that in the other groups (P＜0.05), and the expression of HIF-1α and HIF-1α mRNA in ZBPYR high, middle and low dose group were different statistically (P＜0.05). Conclusion:The ZBPYR could improve the function of neurons though improving oxygen-deficiency in local spinal cord tissue after injury.关键词：spinal cord injury;Zibu Piyin Recipe;hypoxia inducible factor-1α;Western-blot;RT-PCR
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发布时间：2024-01-04 - 摘要：Objective:Investigate the influence of Qianlie Shuang granule on intereukin-10(IL-10),tumor necrosis factor-α(TNF-α) in serum of chronic abacterial prostafitis(CAP)rats. Method:Sixty aged sprague dawley (SD) rats were randomly divided into six groups:control group, model group, Qianlie Huichun granule group, Qianlie Shuang low dose group, Qianlie Shuang medium dose group and Qianlie Shuang high dose group. The changes of prostate gland inflammation was investigated. The expression of IL-10 and TNF-α in rat prostatic tissue was detected by immunohistochemistry. Result:The inflammation of model group were similar to those in clinical CAP, the inflammation of treatment groups were obviously degraded. The IL-10 and TNF-α in model group were obviously higher than in control group(P<0.05).The TNF-α in Qianlie Huichun granule group, Qianlie Shuang medium dose group and high dose group were obviously lower than in model group. The IL-10 in those groups were obviously higher than in model group. Conclusions:Qianlie Shuang granule can regulate the secretion of inflammatory cytokines, which can be one of the mechanisms in the therapy of CAP.关键词：prostatitis;Qianlie Shuang granule;immunohistochemistry;intereukin-10;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective:To observe of Huoxue Dingtong decoction combined with western medicine in the treatment of coronary heart disease and angina pectoris with symptom of heart blood stasis. Method:Eighty cases of coronary heart disease and angina pectoris patients with symptom of heart blood stasis were randomly divided into two groups, the control group and the observation group, with 40 cases in each group. Two groups of patients were given nitrates, antiplatelet drugs and betaloc, vasodilators, lipid-lowering, hypoglycemic treatment. The observation group based on the above combined with Huoxue Dingtong decoction, 1 dose daily and 10 d as a course. Angina pectoris, traditional Chinese medicine(TCM) syndrome, electrocardiogram and curative efficacy were evaluated. Result:For angina pectoris, significant efficiency was 85% in the observation group, and 70% in control group, showed significant difference (P<0.05); the total efficiency was 95% in observation group, and 80% in the control group, showed significant difference (P<0.05). For comparison of electrocardiogram(ECG), significant efficiency was 85.50% in observation group, and 62.50% in control group, showed significant difference (P<0.05); the total efficiency was 95% in observation group, and 77.50% in the control group, showed significant difference (P<0.05). For therapeutic efficacy of TCM syndromes, significant efficiency was 85.50% in observation group, and 62.50% in control group, showed significant difference (P<0.05); the total efficiency was 95% in observation group, and 77.50% in the control group, showed significant difference (P<0.05). Conclusion:Huoxue Dingtong decoction combined with western medicine in the treatment of coronary heart disease and angina pectoris with symptom of heart blood stasis appears to be effective.关键词：coronary heart disease;angina pectoris;heart blood stasis;Huoxue Dingtong decoction
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发布时间：2024-01-04 - 摘要：Objective:To study the clinical efficacy and mechanism of action for treating menopausal women with depression by Zuogui wan and anti-depression wan. Method:Ninety patients were randomly divided into treatment and control groups (n=45 each). Control group were treated with duloxetine 20 mg, tid, orally. The treatment group was given Zuogui wan, 9 g, bid orally and anti-depression wan, 4 g, tid, orally, for 8 weeks. The Hamilton Depression Rating Scale (HAMD), the Pittsburgh Sleep Quality Index Scale (PSQI), serum estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and serum 5-hydroxy tryptamine(5-HT) were used to evaluate the efficacy. Result:After 8 weeks treatment, HAMD and PSQI scores in the treatment group were lowered compared with those in the control group (P<0.01). In the treatment group, level of E2 was significantly increased, higher than that in the control group (P<0.01). FSH and LH levels in the two groups were significantly lowered. The levels of FSH and LH were significantly lowered in the treatment group compared with those in the control group (P<0.05, P<0.01). Serum 5-HT levels were significantly elevated in the two groups, the level in treatment group was not increased as that much as in the control group (P<0.05). The total effective rate of treatment group(91.11%) was better than that in the control group(75.55%) (P<0.05). Conclusion:Zuogui wan and anti-depression wan are effective and safety for treating menopausal women with depression.关键词：depression;menopause;Zuogui Wan;anti-depression wan
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of Kang'ai injection combined with chemotherapy in the treatment of malignant tumor for the clinical curative effect. Method:Eighty cases of malignant tumor were randomly divided into observation group and control group with 40 cases in each group. The two groups were given pemetrexed and cisplatin combined chemotherapy. The observation group was additionally given Kang'ai injection (5% glucose injection 250 mL+Kang'ai injection 50 mL, intravenous infusion, daily) for 30 d. Result:The 1 year survival rate in observation group was 65%, while in control group was 45%, two groups had significant difference (P<0.05). The 3 year survival rate in observation group was 37.50%, while in control group was 25%, two groups had significant difference (P<0.05). The scores for physical health, mental health, social relations, environmental factors after treatment in the observation group were (25.7± 6.2), (23.3 ±3.7), (23.7 ±6.4) and (22.4 ±4.6), while in the control group were (15.7± 6.2), (14.3 ±3.7), (14.7 ±6.4) and (17.4 ±4.6), the observation group was better than the control group (P<0.05). Though two groups were significantly improved (P<0.05). After treatment,IL-6, TNF-α in observation group were (445.74± 96.22), (393.35 ±83.57) ng·L-1, while those in control group were (385.44± 79.26), (358.33 ±73.82) ng·L-1, two groups were significantly improved (P<0.05) and the observation group was better than the control group (P<0.05). After treatment CD3+, CD4+, CD8+and CD4+/CD8+in the observation group were(55.54±8.25) %, (38.43 ±8.87) %, (23.54 ±6.42) % and (1.34±0.25), those in the control group were (49.35±10.25)%, (34.35±8.32)%, (28.52±7.45) %, and (1.20±0.16). Two groups were significantly improved (P<0.05), while the observation group was better than the control group (P<0.05). Conclusion:Kang'ai injection combined with chemotherapy in the treatment of malignant tumor shows significant curative effect. The therapy can prolong the survival of patients, improve the quality of life.关键词：malignant tumor;Kang'ai injection;chemotherapy;immune function
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发布时间：2024-01-04 - 摘要：Objective:To investigate the changes of vascular endothelial function in patients with non-alcoholic fatty liver disease(NAFLD) and the intervention of Silybin Capsule. Method:Sixty patients with NAFLD were selected and randomly divided into 2 groups, routine group (n=30) was treated with vitamin E capsules(100 mg, tid); the Bailing Capsule group (n=30) was treated with Silybin Capsule(105 mg, tid). Another 30 healthy patients as control group. After 3 months treatment, the endothelium-dependent flow-mediated dilatation(FMD) of brachial artery was determined with ultrasound, and the levels of body mass index(BMI),alanine aminotransferase(ALT),aspartate aminotransferase(AST),cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), high sensitivity C-reactive protein (hs-CRP), free fatty acids(FFA),insulin resistance index (HOMA-IR)and endothelin-1(ET-1) in three groups were measured respectively before and after treatment. Result:All patients with NAFLD showed that the BMI,ALT,AST,TC,TG,LDL-C,hs-CRP,HOMA-IR,FFA and ET-1levels were higher thantheseof the controlled group(P＜0.05), the HDL-C and FMD levels were lower than those of the controlled group(P＜0.05). After treatment the hs-CRP,HOMA-IR,FFA,ET-1 level in the Silybin Capsule group·L-1, HOMA-IR (3.34±0.58), FFA (0.52±0.21)mmol·L-1,ET-1(98.7±10.6) ng·L-1] was decreased more significantly than routine group·L-1,(3.62±0.74),(0.68±0.16)mmol·L-1,(160.5±11.78) ng·L-1] (P＜0.05). After treatment the FMD in the Silybin capsule group was increased more significantly than routine group (P＜0.05). Conclusion:The Silybin Capsule can effectively reduce the body's insulin resistance,inflammationrecovery and recovery vascular endothelial function in patients with NAFLD.关键词：non-alcoholic fatty liver disease;endothelial dysfunction;insulin resistance;inflammation;silybin
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发布时间：2024-01-04 - 摘要：Objective:To observe the curative effect of Jianpi Yiqi Tang Combined with medicated diet in treating fatty liver. Method:A total of 100 patients with fatty liver who were randomized to be divided into 2 groups,50 for each group. The therapeutic group with traditional Chinese medicine(TCM), The control group with western medicine routine treatment,2 months later, clinical symptom, Laboratory parameters including glycosylated hemoglobin (HBA1C), fasting serum blood lipid and B-ultrasound were observed to evaluation its clinical effect. Result:The effective rate is 92.0% of the therapeutic group and 64.0% of the control group. There are obvious difference between the groups(P<0.05),the effective of the therapeutic group were evident. There are also obvious difference between the groups in glutamate-pyruvate transaminase(ALT), glutamyl transferase (GGT),total serum cholesterol(TC), serum triglyceride (TG),(P<0.05). Conclusion:Jianpi Yiqi Tang Combined with medicated diet in treating fatty liver are affirmed.关键词：Jianpi Yiqi Tang;Fatty liver;traditional Chinese medicine therapy;medicated diet
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发布时间：2024-01-04 - 摘要：Objective:To investigate the otherness and concertedness between chiness patent medicine Sanjin tablet and antibiotics for treatment of urinary tract infection. Method:After 192 outpatients were divided into three groups by random(Sanjin tablet group,antibiotic group,antibiotic plus Sanjin tablet group),They received treatment and took in germiculture,medicine sensitive trial,immuneglobulin and alexin survey,blood routine,urine routine and were observed in symptom variations. Result:Sanjin tablet have a immunization,lower drug resistance rate,higher moderate drug sensitive rate,lower drug sensitive rate,lower cure rate and near effective rate comparing with antibiotics.Both being prescribed in urinary tract infection are suprior to each adhibition. Conclusion:Antibiotics plus Sanjin tablet are best choice in treatment of urinary tract infection.关键词：Sanjin tablet;antibiotics;urinary tract infection
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发布时间：2024-01-04 - 摘要：Objective:To investigate the clinical applications of Yunnan Baiyao powder (YBP) after cervical cold knife conization (CKC). Method:One hundred and twenty one cases of cervical intraepithelial neoplasia(CIN) grade III treated with CKC were randomly divided into postoperative suture group (control group, n=60) and non-suture YBP packing group (observation group, n=61). The control group was treated with CKC postoperative wound electrocautery, then followed by improved Sturmdorf law suture forming. The observation group was treated with wound electrocautery, and then given 4 g of YBP for spray and gauze. They were followed up for 2 years. The amount of postoperative bleeding, postoperative bleeding time, the average vaginal discharge fluid time and subsequent bleeding, cervical infections and cervical stenosis and other complications were observed. Result:The surgical duration, intraoperative and postoperative bleeding in the observation group were less than those in the control group (P<0.01). The mean bleeding time and the average time of vaginal discharge had no significant difference between the two groups; 5 patients (8.2%) had postoperative secondary bleeding in the observation group while the control group were 4 cases (6.6%), and there were no significant differences; during the follow-up period, there were no cervical infection and cervical stenosis. Conclusion:CKC without suture wound and YBP after electrocautery for stopping bleeding can significantly reduce the operation time, reduce the intraoperative blood loss, it has low incidence of postoperative wound bleeding again. However, it does not increase postoperative infection and cervical canal stenosis and other complications. YBP is worth to be promoted for the application.关键词：Yunnan Baiyao powder;cervical intraepithelial neoplasia;cold knife conization
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发布时间：2024-01-04 - 摘要：The origin and pharmacological action of Jiegeng tang were reviewed and analyzied. The principle of composition, history, clinical practice and pharmacological action of Jiegeng tang were summarized and classified by consulting the CNKI database from 1992 to 2012.Jiegeng tang, the basic prescription for treating sore-throat and highly used in clinic, was originated from (Shanghan Lun). Modern pharmacological studies have shown that it was endowed with quite evident anti-inflammatory and expectorant effects. It is suggest that chemical composition analysis, material basis of therapeutic effects and proportion and mechanism of compatibility of Jiegeng tang should be proceeded in order to provide a basis of clinical application and further development.关键词：Jiegeng tang;Platycodonis Radix;Glycyrrhizae Rodix Et Rhizoma
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发布时间：2024-01-04 - 摘要：Process on drug delivery for brain targeting was summarized to provide basis for traditional Chinese mediline (TCM).Literatures about the development of brain-targeted preparation in recent years were reviewed and analyzed. This paper not only described the physiological structure characteristics of blood-brain-barrier, but also introduced some common methods for promoting drugs through the blood-brain-barrier.The paper also described the administration methods and new preparations(β-CD, prodrug,liposomes,nanoparticles, micro emulsion,in situ gel)to understand the advantages and feasibility of traditional Chinese medicine(TCM) nasal administration. Brain-targeted preparations of TCM had wide development prospect.关键词：blood-brain-barrier;brain-targeted preparation;administration methods;new preparations
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发布时间：2024-01-04 - 摘要：To summarize the relevant research on curcumin in treatment of rheumatoid arthritis and provide useful references for the further research. Literatures were collected inform CNKI and PubMed databases from 1992 to 2012.In the pathological process of rheumatoid arthritis, curcumin could influence synovial fibroblasts,cell factor,matrix metalloproteinases and signal pathways.Up to now, there is no systemic summary on the relation between curcumin and rheumatoid arthritis, this paper provides a foundation for the application of curcumin in treatment pd rheumatoid arthritis.关键词：curcumin;rheumatoid arthritis;cytokine;synoial fibroblast;matrix metalloproteinases;signal path
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发布时间：2024-01-04 - 摘要：To summarize the research progress of chemical structures and biological activities of Buxus alkaloids, which are rich in plants of genus Buxus and provide useful references for the further research. Literature about Buxus alkaloids between 1992 to 2011 in plenty of journals and books were referred to, and the progress of chemical structures and biological activities were summarized. Since 1992, more than 100 alkaloids have been isolated from 8 kinds of plants of genus Buxus. Many researches suggested some of Buxus alkaloids exhibit biological activities including anti-HIV activity, antibacterial, antimycobacterial, antimalarial, antitumor, and inhibitory activities against acetylcholinesterase(AChE),butyrylcholinesterase (BuChE) and glutathione S-transferase (GST). There are more than 70 kinds of plants of genus Buxus all over the world, but only a minority of them has been studied at present. And little research about biological activities of Buxus alkaloids have been reported.In order to develop the medicinal application of the genus of plants, chemical constituents together with pharmacological activities and mechanism should be conducted. Meanwhile, the biological effects and the medical value compounds will be clarified.关键词：Buxus;Buxus alkaloids;chemical constituents;biological activity
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发布时间：2024-01-04 - 摘要：This article mainly expounds the common solubilization polysorbate 80 (Tween 80﹚in the adaptability in Chinese medicine liquid formulation. Polysorbate 80's increase soluble object, quality and standard etc were discussed. Polysorbate 80 is widely used as a solubilizer, emulsifier and stabilizer in the liquid formulations of traditional Chinese medicine for decades. In recent years,people put more attention on the quality of pharmaceutical excipients and their safety because injection has a serious security problem. Because of its wide application in its production processes,product quality and safety research in vitro of tween 80 in the injection become the focus. More studies are needed on Chinese medicine liquid formulation and the safety problems of tween 80 in production and application.关键词：tween 80;solubilization;liquid preparation of traditional Chinese medicine
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发布时间：2024-01-04 - 摘要：Monomers isolated from Tripterygium Wilfordii are mainly chemical compositions including two terpene, three terpene, half terpene, alkaloids, etc. The monomers show effectiveness in suppression of autoimmune diseases as well as anti-inflammatory and antitumor activity. Recent reports in the literatures show that triptolide and celastrol isolated from Tripterygium Wilfordii could inhibit glioma growth by ways of tumor cell apoptosiss, inhibition to tumor cell proliferation and differentiation and refrainning tumor angiogenesis in a dose-effect manner. The mechanism involves regulating expression of Bcl-2, Bax, NF-kappa B and down-regulating Ras/Akt and Ras/ERK signal pathways. They could reduce the RNA polymerase transcription and lower the vascular endothelial cells (EC) proliferation, transference, and the ability for forming small tube by inhibiting vascular endothelial growth factor (VEGF) receptor. The studies on bio-responses and the related molecular mechanism of the monomers will certainly help to illustrate the pharmacologic mechanism of actions. The future emphases will be the development of new monomers with high efficacy and low toxicity.关键词：glioma;Tripterygium Wilfoudii;triptolide;celastrol
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发布时间：2024-01-04 - 摘要：To explore the features of clinical application of the forbidden incompatible pair of Sargassum and Radix et Rhizoma Glycyrrhizae in one formula recorded in contemporary literatures in the recent 60 years (1949-2009). Relation rules were utilized to release the regulations of their computability, accompanied with statistic analysis of their dosages and primary targeted diseases. The core drugs in the formulas containing the incompatible pair were Thallus Laminariae, Radix Angelicae Sinensis, Radix Bupleuri, Spica Prunellae, Concha Ostreae and Pericarpium Citri Reticulatae; the core drug pairs are Thallus Laminariae-Radix et Rhizoma Glycyrrhizae, Thallus Laminariae-Sargassum, Radix Angelicae Sinensis-Radix et Rhizoma Glycyrrhizae, Radix Angelicae Sinensis-Sargassum. Mastosis and thyopathy are the most common diseases treated by this incompatible pair; the dose of Sargassum and Radix et Rhizoma Glycyrrhizae in the formulas are 10-15 g and 3-9 g respectively; their proportion is usually from 7:3 to 8:2. The forbidden incompatible pair of Sargassum and Radix et Rhizoma Glycyrrhizae is often combined with Chinese medicines for activating blood and resolving phlegm to treat mastosis and thyopathy, and the dose of Radix et Rhizoma Glycyrrhizae is smaller than Sargassum.关键词：the 18 forbidden incompatible drugs;Sargassum and Radix et Rhizoma Glycyrrhizae;regulation rules
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