最新刊期
卷 18 ， 期 17 ， 2012
- 摘要：Objective: Using Traditional Chinese Medicine Inheritance System(TCMIS) to analyze the composing principles of the prescriptions for consumptive diseases collected in the book of 〈The Prescription of Traditional Chinese Medicine Dictionary〉. Method: The prescriptions used for consumptive diseases in the book of 'The Prescription of Traditional Chinese Medicine Dictionary'were inputted into the TCMIS. By using revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering, the composing principles were analyzed. Result: Based on the analysis of 99 cases of prescriptions, the frequency of each herb included in the database was computed, Twenty core combinations and 10 new prescriptions wre mined from the database. Conclusion: The composition principles in 〈The Prescription of Traditional Chinese Medicine Dictionary〉 are clarified. TCMIS can be used to input, administrate, search and analyze the prescriptions and is a helpful tool for the research of traditional Chinese medicine.关键词：The Prescription of Traditional Chinese Medicine Dictionary;consumptive diseases;Traditional Chinese Medicine Inheritance System;composition principle
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发布时间：2024-01-04 - 摘要：Objective: To optimize compatibility extraction technology of Ligusticum chunxiong and Angelica sinensis,and compare the content of ferulic acid in Chinese herbal compound contain L. chunxiong and A. sinensis pair compatibility and single one. Method: With the content of ferulic acid as index which was determined by HPLC, compatibility extraction technology of L. chunxiong and A. sinensis was optimized by orthogonal test design with extraction time,extraction voltage,ethanol consumption and ethanol volume fraction as factors,and optimized process was used to extract L. chunxiong and A. sinensis single,the content variation of ferulic acid were compared in compatibility and single extraction,in order to investigate synergism mechanism of compatibility of L. chunxiong and A. sinensis. Result: Optimum extraction technology was:extracted 1.5 min with 20 times the amount of 65% ethanol,extraction voltage 100 V;The content of ferulic acid in compatibility extraction liquid was similar with the total content in single extraction liquid. Conclusion: This optimized technology was simple and economic,it confirmed that reason for enhancing pharmacological effect of compatibility with L. chunxiong and A. sinensis wasn't because of increasing the content of ferulic acid.关键词：flash type extraction;Angelica sinensis;Ligusticum chunxiong;ferulic acid;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: Compound Beimu powder was selected as model drug to investigate preparation and properties of multi-species and multi-texture traditional Chinese medicine (TCM) ultrafine powder in compound. Comparative study of powder characterization and physical properties between fine and ultrafine powder was investigated,in order to provide experimental basis for ultrafine grinding technology using in promotion and application of TCM compound. Method: With particle size and cell wall broken rate as indexes,particle size of drug levels of ultrafine powder was determined by laser particle size distribution instrument,ultrafine grinding process was selected.Apparent characterizations, microscopic appearances, particle size and specific surface areas were compared between fine and ultrafine powder. Meanwhile moisture absorption rate and angle of repose were determined to investigate hygroscopicity and mobility of fine and ultrafine powder. Result: Cell disruption rates were all above 95% when Ussuriensis fritillary,Chinese ephedra, Glycyrrhiza uralensis and Armeniaca amara were pulverized for one hour as well as Radices stemonae was pulverized for 40 minutes.Physical properties studies demonstrated that color of drug had shallow trend and its character was finer and smoother after ultrafine grinding, electron microscope scanning results showed that particle size distribution of ultrafine powder was narrower, simultaneously,some common microscoptical characters disappeared.Mobility of plant medicine reduced, however, mineral medicine increased.Hygroscopicity of U. Fritillary,C. ephedra and G. uralensis all demonstrated decreasing trend. Conclusion: Compared with fine powder,appearance,microscopic characteristics,mobility and moisture absorption of ultrafine powder were changed,this article could provide reference for processing of Chinese herbal ultrafine powder.关键词：compound Beimu powder;ultrafine grinding technology;powder characteristics;physical properties
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发布时间：2024-01-04 - 摘要：Objective: To select optimum macroporous resin for enrichment of triptolide and wilforgine from Tripterygium wilfordii. Method: Methods of determination for triptolide and wilforgine from extract of T. wilfordii by HPLC were established.Optimum type of macroporous resin was selected by static and dynamic absorption with the contents of riptolide and wilforgine as indexes. Result: HPD100 macroporous resin was the best resin of purifing triptolide and wilforgine,rate of static adsorption of triptolide and wilforgine was 92.75% and 84.84%, respectively. Rate of dynamic adsorption of triptolide and wilforgine was 92.95% and 90.02%, respectively. Conclusion: HPD100 macroporous resin could be used to purify extract of T. wilfordii.关键词：Tripterygium wilfordii;macroporous resin;select;static adsorption;dynamic adsorption
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology for total flavonoids from Gynostemma pentaphyllum by polyamide resin. Method: With the content of total flavonoids as index,UV spectrophotometry was used, adsorption and elution behavior of polyamide resin for total flavonoids was investigated under static and dynamic conditions, and to select optimum technology parameters of purification technology for total flavonoids by polyamide resin. Result: Optimum purification technology parameters were:at room temperature,the concentration of crude drug 0.35 g·mL-1,pH 3.0 and 4 BV 60% ethanol as eluant. Under these conditions,purity of total flavonoids increased from 23.91% to 81.97%,retention rate of total flavonoids in the purified was up to 90.11%. Conclusion: Purified effect of total flavonoids from G. pentaphyllum by polyamide resin was preferable,it was suitable for industrial production.关键词：Gynostemma pentaphyllum;total flavonoids;polyamide resin;purification
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology conditions of oleanolic acid from Chaenomeles speciosa by supercritical CO2. Method: HPLC was used to determine the content of oleanolic acid, with average between yield and content of oleanolic acid as index,single factor test and orthogonal test were used to investigate extraction temperature, extraction pressure, separation temperature, separation pressure and kind of carrying agent,and compared with optimized process and documentation process. Result: Optimum extraction technology conditions were:extraction temperature of 45 ℃,extraction pressure of 40 MPa,separation temperature of 30 ℃,separation pressure of 8 MPa and carrying agent of 95% ethanol;Supercritical CO2 extraction was superior to other documentation methods. Conclusion: This optimized technology was feasible and stable, it could be used to promote application of oleanolic acid.关键词：supercritical CO2;extraction;Chaenomeles speciosa;oleanolic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total tritepenoidic acid from Fomes officinalis and establish its content determination method. Method: The content of total tritepenoidic acid was determined by UV-Vis with oleanic acid as reference;Effects of kind and concentration of extraction solvent,solid-liquid ratio, temperature, extraction time and extraction times on yield of total tritepenoidic acid were investigated by single factor test,basis on this,extraction technology was optimized by orthogonal test. Result: Optimum conditions of ultrasonics extraction were as follow:extracted 3 times at 60 ℃ with 30 times the amount of 90% ethanol,30 min each time.Under these conditions,effect of factors on yield was in order of extraction temperature＞solid-liquid ratio＞extraction time＞the concentration of ethanol,yield of total tritepenoidic acid from F. Officinalis was 35.1%. Conclusion: The content of triterpenoidic acid from F. officinalis was very high, this optimized technology was stable and feasible,which could be extended to large-scale production.关键词：Fomes officinalis;total tritepenoidic acid;orthogonal experiment;ultrasonic extraction
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发布时间：2024-01-04 -
Technology Optimization of Inclusion Compound for Volatile Oil from with Hydroxypropyl--cyclodextrin
摘要：Objective: To optimize preparation technology of inclusion compound of volatile oil from Cnidium monnieri with hydroxypropyl-β-cyclodextrin (HP-β-CD). Method: With utilization rate of volatile oil and yield of inclusion compound as indexes,inclusion compound of volatile oil from C. monnieri with HP-β-CD was optimized by orthogonal design.Quality control of inclusion compound was studied by UV technology,microscopic identification and GC-MS analysis. Result: Optimum inclusion technology was:volatile oil to HP-β-CD 1∶8(mL·g-1), inclusion time 2.0 h,inlusion temperature 40 ℃.It verified that UV absorption spectrum was the same before and after inclusion,volatile oil had been inclusion by microscopic identification,GC-MS verified that composition in volatile oil was essentially unchanged before and after inclusion. Conclusion: Both of extraction and inclusion technology were reasonable and feasible,it could provide experimental basis for rational use of clinical formulation and development of inclusion.关键词：volatile oil from Cnidium monnieri;hydroxypropyl-β-cyclodextrin;inclusion compound- 2
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发布时间：2024-01-04 - 摘要：Objective: To optimize processing technology of Glycyrrhiza uralensis-processed Evodia rutaecarpa. Method: Fingerprint technique of hydrophilic and lipophilic components in E. rutaecarpa was used, single factor test was employed to investigate effect of four factors including the amount of G. uralensis,moistening time, frying temperature and time on processing technology of E. rutaecarpa. Result: Frying temperature and time had significant effect on fingerprint of E. Rutaecarpa,optimum processing technology was as follows:ratio of G. uralensis to raw material 6∶100,fryed 10 min at 180 oC after moistening 3 h. Conclusion: This optimized technology was stable and feasible,it could provided a scientific basis for standardization for processing technology of G. uralensis-processed E. rutaecarpa.关键词：fingerprint;processed Evodia rutaecarpa;processing technology;Glycyrrhiza uralensis
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of Huanglian ointment. Method: With yield of extract,the content of total alkaloid and composite score as indexes,extraction technology was optimized by orthogonal test with the concentration of ethanol, solid-liquid ratio, extraction time and extraction times as factors; Effect of five different ointment matrix prescriptions on formation and quality of Huanglian ointment was compared by stability experiment. Result: Optimum extraction process was:extracted 2 times with 6 times the amount of 70% ethanol, 1.5 h per time;Ointment matrix composition as follows:octadecanol, liquid paraffin, white petrolatum, glycerin monostearate, Tween-80, Span-60, glycerin,double distilled water. Conclusion: This optimized extraction technology and ointment molding process was stable and feasible,it could be applied to large-scale production.关键词：orthogonal design;extraction process;ointment;prescription
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of theanine from tea stalk. Method: With the content of theanine as index which was determined by HPLC,ratio of liquid-material,extraction solvent,extraction temperature, extraction time and extraction times were selected as factors,factors and levels were determined by single-factor test; Orthogonal test was used to optimize extraction technology with ratio of liquid-material,extraction temperature and extraction time as factors. Result: Optimum extraction process was:extracted 1 h with 20 times the amount of water at 80 ℃. Conclusion: This optimized technology was stable and reliable with high extraction rate.关键词：tea stalk;theanine;single-factor test;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To optimize water extraction technology of Xiaoshi Daozhi gel. Method: With the content of geniposide as index which was determined by HPLC,Agilent XDB-C18 column(4.6 mm×250 mm,5 μm), acetonitrile-water(10∶90)as mobile phase with flow rate of 1.0 mL·min-1,UV detection wavelength was 238 nm, orthogonal test was used to optimize extraction technology of Xiaoshi Daozhi gel with water volume,extraction times and extraction time as factors. Result: Optimal water extraction technology was as follows:extracted 2 times with 6 times the amount of water,1 h per time. Conclusion: This optimized technology was simple with low cost,it was suitable for industrial production.关键词：Xiaoshi Daozhi gel;gardenoside;orthogonal test;HPLC;extraction process
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Shuru gel patch. Method: With the amount of extract and the content of cinnamic aldehyde as indexes,the content of cinnamic aldehyde was determined by HPLC,extraction technology of Shuru gel patch was optimized by orthogonal test,three factors were investigated including soaking time,water volume and extraction time. Result: Optimal extraction technology was:soaked 0.5 h,and extracted 5.5 h with 8 times the amount of water,collected volatile oil. Conclusion: Under this optimized technology,yield of extract and cinnamic aldehyde were high,it was available for industrial production.关键词：Shuru gel patch;extract yield;cinnamic aldehyde;Orthogonal Design;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of dihydromyricetin from Ampelopsis grossedentata by response surface methodology. Method: With extraction ratio of dihydromyricetin as index,based on single factor test,the concentration of ethanol, liquid-solid ratio and extraction time were selected as independent variables and extraction rate of dihydromyricetin as response value,response surface methodology with three-factors and three-levels was carried out for optimizing extraction process of dihydromyricetin from A. grossedentata. Result: Optimum extraction conditions of dihydromyricetin were as followed:refluxing extracted 95 min with 17 times the amount of 81% ethanol. Under these conditions,extraction ratio of dihydromyricetin was 9.58%,relative error in comparison with the predicted one was 0.14%. Conclusion: This optimized technology was reliable,it showed that was feasible for optimizing extraction technology of dihydromyricetin by response surface methodology.关键词：response surface;Ampelopsis grossedentata;dihydromyricetin;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To investigate molding process of fresh Rehmannia glutinosa formula granule. Method: Kinds and consumption of accessories were investigated with particle size,bulk density,mobility,melting and moisture absorption as indexes;The concentrations of ethanol and optimum wetting agent were investigated with soft material properties,difficulty degree of granulation,product character as indicators. Result: Optimum granulation process was:mixing ratio of dry powder-maltodextrin 1∶1,85% ethanol as wetting agent to make soft material, extrusion granulation with one drug sieve,dried at 60 ℃,sorting particle.Critical relative humidity of product particles was 60%. Conclusion: This optimized process was simple with high yield,it could provide experimental basis for large-scale production of fresh R. glutinosa formula granule.关键词：fresh Rehmannia glutinosa formula granule;molding process;accessories
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发布时间：2024-01-04 - 摘要：Objective: To optimize macroporous resin for purifying total flavonoids from Chrysanthemum morifolium and its technology conditions. Method: Using the content of total flavonoids as index which was determined by UV spectrophotometry,adsorption and desorption performance of HPD100, DM130, D101, AB-8 four macroporous resins for total flavonoids from C. morifolium were investigated,in order to screen optimum macroporous resin and optimize its separation technology conditions. Result: Adsorption capacity and desorption rate of AB-8 was the most in these resins, values were 44.9 mg·g-1 and 87.9%,respectively. Optimum separation technology was:the content of total flavonoids from sample solution 12.6 g·L-1, desorption time 1 h at 30 ℃,95% ethanol as eluent. Conclusion: AB-8 resin was suitable for adsorption and separation of total flavonoids from C. morifolium.关键词：macroporous resin;Chrysanthemum morifolium;total flavonoids;adsorption;separation
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发布时间：2024-01-04 -
Optimization of Paeonol Tablets Formulation by Central Composite Design-Response Surface Methodology
摘要：Objective: To optimize the formulation of paeonol tablets by the central composite design-response surface methodology. Method: In the formulation design using response surface methodology plus central composite design,independent variables included the amounts of MCC102,Cellactose 80, PVP S63 and the tablet hardness.Disintegration time and friability of tablets were taken as dependent variables, and overall desirability was calculated from the geometric mean of the two desirabilities of each formulation.Multilinear, quadratic and cubic models were used to estimate the relationship between the dependent and the independent variables,and to delineate response surface method and overlay contour plots in order to select the optimal formulations.Finally,predicted responses were verified. Result: The quantitative relationships between three factors and 3 evaluation indexes were characterized.Optimal formulation was proposed to contain 1% PVP S630,the proportion of MCC 102 and Cellactose 80 was 1∶1.Dissolution test of the selected optimal formulation indicated that there existed high approximation between the observed and estimated values. Conclusion: The muti-objective simultaneous optimization of paeonol tablets formulation could be achieved by the central composite design and response surface methodology.关键词：paeonol;central composite design response surface;tablet;formulation- 2
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发布时间：2024-01-04 - 摘要：Objective: To optimize film coating process of Xinqingning tablet. Method: Orthogonal test was used with the concentration of coating solution,the spray amount of coating solution,inlet air temperature and rotation of coating pan as factors,technology parameters of coating process were optimized by composite score method. Result: Optimum coating process parameters were:the concentration of coating solution 0.14 g·mL-1,the spray amount of coating solution 5.05-5.15 g·min-1,inlet air temperature 80 ℃,rotation of coating pan 6-8 r·min-1. Conclusion: This optimized technology was stable and feasible,it could be promoted to large-scale production.关键词：Xinqingning tablet;film coating;orthogonal test;composite score
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发布时间：2024-01-04 - 摘要：Objective: To examine the influence on the quality of toad venom processed by different drying and processing methods. Method: Toad venom collected in Nantong,Jiangsu Province, was dried as follows: heated at 105 ℃, heated at 80 ℃, heated at 60 ℃, heated at 60 ℃under the reduced pressure, freeze-dried. The dried toad venom was processed with wine and milk. The content of five indole alkaloids and five bufadienolides was determined by HPLC in the different samples in order to evaluate the best drying method and compare the difference between the crude and processed toad venom. Result: The total content of five indole alkaloids in the samples obtained by different dryness methods was as follows: 17.57%±0.15%, 20.01%±0.45%, 19.99%±0.68%, 19.85%±0.25%, 20.12%±0.27%, respectively. The total content of five bufadienolides in the samples obtained by different dryness methods was as follows: 19.91%±0.17%, 20.20%±0.17%, 19.96%±0.06%, 20.24%±0.17%, 21.05%±0.13%, respectively. The total content of five alkaloids in the toad venom and its processed products with wine and milk was 15.62%±0.29%,15.77%±0.24%,15.78%±0.27%and the total content of five bufadienolides was 20.69%±0.17%,20.74%±0.09%,22.12%±0.21%, respectively. Conclusion: According to the total content of five indole alkaloids and five bufadienolides as well as the appearance of the sample, the freeze-dryness was regarded as the best method, however, comprehensively considering the dryness time and cost, as well as the feasibility of the operation in the production area, heating at 60-80 ℃ for several hours is also an alternative drying method. Processing with wine and milk has no obvious influence on the content of five indole alkaloids and five bufadienolides in toad venom.关键词：toad venom;HPLC;indole alkaloid;bufadienolide;drying method;process method
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for determination of crocin-Ⅰand crocin-Ⅱ in the Gardenia jasminoides. Method: High performance liquid chromatography was applied on a Dikma Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column at temperature 30 ℃ with 0.1% formic acid-acetonitrile as the mobile phase in gradient elution for 30 min. The flow rate was 1.0 mL·min-1, detected wavelength of 440 nm. Result: The sample quality of crocin-Ⅰand crocin-Ⅱwith the peak area showed good linear relationship; the average recoveries were 96.68% (n=6) and 102.94% (n=6). Conclusion: The method is simple, accurate and reproducible; it can provide the basis of rational application, quality control and the standards for G. jasminoides.关键词：Gardenia jasminoides;HPLC;crocin-Ⅰ;crocin-Ⅱ
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发布时间：2024-01-04 - 摘要：Objective: To research the chromatography fingerprint of Radix Astragali with hyphenated technique of HPLC-DAD-ELSD. Method: The chromatography conditions were defined as SpursilTM C18 column (4.6 mm× 250 mm,5 μm); mobile phase was A:ACN, B:H2O, gradient elution; flow speed was 1.0 mL·min-1; temperature of column was set at 25 ℃; injection volume was 20 μL; detected wavelength was at 254 nm. The ELSD conditions were as follows: the temperature of drift tube was 112.8 ℃, the gas speed was 3.2 L·min-1. Result: In the chromatography fingerprint with HPLC-DAD of Radix Astragali, 14 common peaks were demarcated, calycosin and formononetin were identified; in the chromatography fingerprint with HPLC-ELSD of Radix Astragali, 9 common peaks were demarcated, calycosin-7-O-β-D-glucoside, astragaloside Ⅳ, astragaloside Ⅲ and astragaloside Ⅱ were identified. Conclusion: The method was accurate and reliable and the study may contribute in controlling of Radix Astragali.关键词：Radix Astragali;fingerprint of traditional Chinese medicine;HPLC-DAD-ELSD
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of p-hydroxycinnamic in 5 batch Slices of Rhodobryum giganteum by HPLC. Method: DIKMA Platisil ODS C18 (4.6 mm×250 mm, 5 μm) was used with the mobile phase of acetonitrile-1% acetic acid water (20∶80). Flow rate was 1.0 mL·min-1. The detection wavelength was set at 310 nm. Column temperature was maintained at 30 ℃. Result: p-Hydroxycinnamic had a good linear relationship (r=0.999 9) in the 0.08-0.4 μg range, the average recovery was 95.7%-104.0%. The average content of p-hydroxycinnamic in 5 batch slices of R. giganteum determined was 0.111 5 mg·g-1. Conclusion: The method was simple, fast, and had a good separation, high sensitivity, good reproducibility and could be used for the quality control of R. giganteum.关键词：p-hydroxycinnamic;Rhodobryum giganteum;HPLC
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发布时间：2024-01-04 - 摘要： Objective: To establish the method for content determination of sinapine cyanide sulfonate in Semen Raphani total alkaloids. Method: The determination was carried out with HPLC, acetonitrile-H2O-0.08 mol·L-1 KH2P04 (12∶88) as the mobile phase at a flow rate 1.0 mL·min-1. Result: The linear range of Sinapine cyanide sulfonate was 0.482-2.892 μg and the average recovery was 103.63%. Conclution: The method is rapid, simple, accurate and reliable in results,and suitable for the determination of sinapine cyanide sulfonate in Semen Raphani total alkaloids.关键词：Semen Raphani total alkaloids;sinapine cyanide sulfonate;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for Dioscorea nipponica rats serum HPLC fingerprinting. Method: The separation was performed on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with 0.1% phosphoric acid water and acetonitrile as mobile phase by gradient elution; the flow rate was 0.8 mL·min-1; detection wavelength was set at 254 nm; the column temperature was maintained at 25 ℃. Result: The RSD of precision, reproducibility, stability test of relative peak area and relative retention time was less than 5%. Conclusion: The method is simple, practical, reliable,which would lay a solid foundation for further studies on pharmacodynamic material basis of Dioscorea nipponica and pharmacodynamic action mechanism research.关键词：Dioscorea nipponica;RP-HPLC;serum fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To establish the methods for determination of imperatorin and astragaloside in Tongqiaobiyan granule. Method: The HPLC method was performed on Diamonsil C18(4.6 mm×250 mm, 5 μm)column;a mixture liquid of methanol-water(54∶46) was used as the mobile phase,and the detective wavelength was at 238 nm to determine the content of imperatorin. With Philo door 100A C18 (4.6 mm×150 mm, 5 μm)column and evaporative light scattering detector, a mixture liquid of acetonitrile-water (32∶68)was used as the mobile phase to determine the content of astragaloside. Result: The calibration curve of imperatorin and astragaloside were linear in the ranges of 0.072 5-0.773 6, 1.462 8-7.314 μg respectively. Conclusion: It is an accurate and credible quality control method.关键词：Imperatorin;astragaloside;evaporative light scattering detector
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发布时间：2024-01-04 - 摘要：Objective: To establish the methods of qualitation and quantitation about Trichosanthis Semen, one of the commonly used Chinese material medica. Method: The thin-layer chromatography (TLC) method was found with the reference substance both karounidiol and α-spinasterol. The HPLC method was found with the PAD and the reference substance of karounidiol. Result: The unsaponifiable matter from all 12 Trichosanthis Semen samples showed the spots of both karounidiol and α-spinasterol on a TLC plate. The content of karounidiol in all 12 Trichosanthis Semen samples was 0.056 88%-0.087 28%. Conclusion: Karounidiol, one of the active components of Trichosanthis Semen, can be considered as an important index of the quality standard of Trichosanthis Semen. The content of karounidiol was suggested not less than 0.05% in Trichosanthis Semen.关键词：Trichosanthis Semen;karounidiol;TLC;HPLC;Trichosanthes kirilowii
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical components of Styrax tonkinensis. Method: Chromatographic technologies were used for separation and purification, while physicochemical properties and spectral analysis were used for structure elucidation. Result: Eleven compounds were isolated and their structures were identified as 19α-hydroxy-3-oxo-olean-12-en-28-oic acid ( 1 ), 6β-hydroxy-3-oxo-olean-12-en-28-oic acid ( 2 ), sumaresinolic acid ( 3 ), siaresinolic acid ( 4 ), oleanolic acid ( 5 ), 4- -2-methoxyphenol ( 6 ), benzoic acid ( 7 ), vanillin ( 8 ), vanillic acid ( 9 ), coniferyl aldehyde ( 10 ), dehydrodivanillin ( 11 ). Conclusion: Except for compound 7, the other compounds were isolated from S. tonkinensis for the first time.关键词：Styrax tonkinensis;chemical constituents;triterpenoids
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发布时间：2024-01-04 - 摘要：Objective: To explore the correlation between the color and the contents of effective constituents, including chlorogenic acid and galuteolin, in Lonicera japonica . Method: High Performance Liquid Chromatography(HPLC) was applied to determine the contents of chlorogenic acid and galuteolin in L. japonica from different habitats, collecting periods and processing methods. The colorimeter was applied to determine the color of L. japonica. Result: The color index L* of L. japonica mainly showed a negative correlation with chlorogenic acid while no correlation with galuteolin. Conclusion: The obtained method in this study may give insight to quickly estimate and predict the content of chlorogenic acid.关键词：Lonicera japonica;chlorogenic acid;galuteolin;color;colorimeter
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发布时间：2024-01-04 - 摘要：Objective: Four kinds of different active ingredients from the fruiting bodies from different varieties of Ganoderma lucidum have been detected. The experimental data could be used as the basis of the special medicinal varieties breeding. Method: The content of total polysaccharides, triterpenoid and protein was determined by UV spectrophotometer. Ergosterol was determined by HPLC. Result: Total polysaccharides, triterpenoid and protein were the main components of different sources of Ganoderma lucidum. Ergosterol content was very low in the fruiting bodies. The content of the four ingredients was significantly different in different sources of G. lucidum. In addition, there were no correlations among the content of these four ingredients. Conclusion: Determination of polysaccharides, triterpenoid and protein was meaningful in the quality control of G. lucidum, and it can provide scientific basis for the breeding of special varieties.关键词：Ganoderma lucidum;varieties;active ingredients;quality control
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity of 6 compounds isolated from Erigeron breviscapus. Method: Compounds were isolated and acquired from E. breviscapus through the methods of organic solvent extraction and chromatography, and identified by the spectrum of NMR(nuclear magnetic resonance). The scavenging activities of all these compounds against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) radicals, 2,2'-amino-di(2-ethyl-benzothiazoline sulphonic acid-6) ammonium salt (ABTS) radicals,O2-·and·OH radicals were evaluated by in vitro method and compared with Vc as positive control. Result: All the six compounds have scavenging capability against DPPH radicals, ABTS radicals,O2-·and·OH radicals and have a good dose-responses relationship with the concentration. The differences between the free radical scavenging capacity of six compounds and Vc are were significant. Conclusion: Six pounds isolated from E. breviscapus have the stronger antioxidant activity.关键词：Erigeron breviscapus;free radicals;scavenging activity;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To establish a dual-wavelength high performance liquid chromatography method for uncertainty analysis of chlorogenic acid, forsythin and aicalin in Shuanghuanglian Oral Liquid. Method: According to the technical requirements of JJF 1059—1999(Evaluation and expression of uncertainty in measurement), each active component of uncertainty was calculated the variable parameters among the procedures.The combined uncertainty and extended uncertainty and reported uncertainty were finally obtained by synthesized the uncertainties of various component variables. Result: Uncertainty evaluation for chlorogenic acid was ±2.23%, forsythin was ±9.18% and aicalin ±0.84%. Conclusion: The uncertainty of the reference standard quality was the main source of three components measurement uncertainty in Shuanghuanglian Oral Liquid.关键词：dual-wavelength HPLC;shuanghuanglian oral liquid;chlorogenic acid;Forsythin;aicalin;measurement uncertainty
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发布时间：2024-01-04 - 摘要：Objective: To compare the diversity of curcumin content in Curcuma longa before and after processed. Method: The column of HPLC was SHIMADZU VP-ODS C18 (4.6 mm×250 mm,5 μm), the mobile phase was acetonitrile-0.1% acetic acid (48∶52),V=1.0 mL·min-1, λ= 430 nm,t=30 ℃. Volatile oil was extracted with steam distillation. Result: The content of curcumin in different processed products of C. longa was as folllows: raw materials(10.77 mg·g-1)>slightly fried sample(10.04 mg·g-1)>primitive(10.02 mg·g-1)>liquor-fried sample (9.44 mg·g-1)>vinegar-fried sample (9.28 mg·g-1). The content of volatile oil was as follows: raw materials(8.05%)>primitive(8.00%)>liquor-fried sample (7.99%)>slightly fried sample(7.81%)> vinegar-fried sample(7.60%). Conclusion: Different processed methods may have some affection to the quality of C. longa.关键词：Curcuma longa;processing;volatile oil;curcumin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC-ELSD method for the determination of saponin A in Reineckia carnea. Method: The column was Elipse-XDB-C18 (4.6 mm×150 mm, 5 μm) and mixture of methanol-water (46∶54) as mobile phase; the flow rate was 1.0 mL·min-1and the column temperature was at 40 ℃. Drift tube temperature was 40 ℃, pressure of nebulizer gas was 0.3 MPa. Result: The linearity of saponin A was good in the range of 0.618-9.888 μg(r=0.999 8). The average recovery was 99.11%, with RSD of 2.47%. Conclusion: The method is convenient, rapid, accurate with high sensitivity and can be used reliably for analyzing saponin A in R. carnea.关键词：Reineckia carnea;saponin A;HPLC-ELSD
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发布时间：2024-01-04 - 摘要：Objective: To evaluate accumulation of the volatiles of the different tissues in Magnolia officinalis. Method: The volatiles of leaf buds, root skins, barks, twig skins, leaves, pericarps and seeds of M. officinalis were analyzed using headspace solid phase micro-extraction(HS-SPME) following by capillary gas chromatography coupled to mass spectrometry (GC-MS). Result: The volatiles were α-linalool 0.21%-4.92%, α-caryophyllene 7.48%-38.71%, β-caryophyllene 1.53%-14.43%, eudesma-4(14),11-diene 0.15%-10.02%, β-chamigrene 0.56%-10.68%, caryophyllene oxide 1.68%-36.01%, α-eudesmol 1.56%-33.79% and β-eudesmol 0.19%-28.89%. The yields of essential oils in the different tissues were 0.12%-0.96%. Conclusion: The essential oil, the volatiles composition and their accumulation content of leaf buds, root skins, barks, twig skins, leaves, pericarps and seeds of M. officinalis were significantly different.关键词：Magnolia officinalis;tissue;volatiles;evaluation
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发布时间：2024-01-04 - 摘要：Objective: To study the secondary metabolites of endophytic fungal strain Botryosphaeria sp. MHF of Maytenus hookeri. Method: The chemical constituents were isolated by column chromatography such as normal phase or reverse phase etc. The structures were identified by spectroscopic analysis. Result: Eight compounds were obtained and elucidated as 22E,24R-ergosta-5-en-3β-ol ( 1 ), 22E,24R-ergosta-4,6,8,22-tetraen-3-one ( 2 ), 22E,24R-3β,5α,9α-trihydroxy-ergosta-7,22-diene-6-one ( 3 ), 22E,24R-ergosta-7,22-diene-3β,5α,6β-triol ( 4 ), 22E,24R-5α,8α-epidioxyergosta-6,22-dien-3β-ol ( 5 ), fusaproliferin ( 6 ), cerebroside C ( 7 ) and 3,4,5-trihydroxy1-tetralone ( 8 ). Conclusion: All these compounds were isolated from this strain cultivated on Murashige-Skoog culture medium for the first time.关键词：Maytenus hookeri;endophytic fungal strain;Botryosphaeria sp. MHF;chemical constituents;ergosterol derivatives
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the preparation of rhamnocitrin reference substances from Oxytropis falcata. Method: Separated using silica gel column chromatography preliminarily, and then separated and purified rhamnocitrin by Sephdex LH-20 column, thin-layer chromatography and area normalization of HPLC was used to detect purity, spectral analysis identified its structure. Result: The preparation of compounds of rhamnocitrin, had a purity of more than 98%. Conclusion: The preparation method has high efficiency, the reference substance induced has high purity and meet the relevant technical requirements of traditional Chinese medicine chemical reference substance, can be used for mass production of the reference substance used for traditional Chinese medicine qualitative and quantitative analysis.关键词：Oxytropis falcata Bunge;rhamnocitrin;reference substances
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发布时间：2024-01-04 - 摘要：Objective: To develop a RP-HPLC method for the quantitative analysis of daurisoline and dauricine, and establish an HPLC-UV fingerprint of Rahizoma Menispermi. Method: The alkaloids were separated on a ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm)with gradient elution using acetonitrile and H2O-formic acid (100∶0.5)solvent system and detected at 268 nm. Flow rate was 1.0 mL·min-1 and 10μL was injected every time. Result: The linear range of daurisoline and dauricine was 0.072-2.29 mg·mL-1(r=0.999 6),0.053-1.70 mg·mL-1(r=0.999 9), respectively. The average recovery rates of daurisoline and dauricine were 98.7% (RSD 1.5%),100.6%(RSD 1.9%), respectively. In the fingerprint, 9 common peaks of Rahizoma Menispermi from Chengde and 13 common peaks of Rahizoma Menispermi from other production areas were confirmed. The accuracy and reproducibility of the method of fingerprint were good. The results of similarity analysis were above 0.9 except No.2 was 0.404 of Rahizoma Menispermi from Chengde and No.9 was 0.716 from other production areas. Conclusion: The method can be used scientifically to evaluate the quality of the Rahizoma Menispermi qualitatively and quantitatively.关键词：Rahizoma Menispermi;daurisoline;dauricine;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint of mongolian medicine sedeng and provide reliable method for scientific evaluation and effective control of quality. Method: Sendeng from 10 different area was determined by HPLC-DAD, and evaluated with traditional Chinese medicine fingerprint similarity evaluation system 2004A, the Common Pattern was established and the similarity of chromatography with similar degree was evaleated. Result: The HPLC fingerprint of sedeng from 10 different area had 10 common peak, the similar degree of HPLC fingerprint was above 0.9; the similarity of Fingerprint of Mongolian Medicine Sedeng from different area were good. Conclusion: The method can be used to evaluat the quality of Mongolian medicine sedeng.关键词：Mongolian medicine sedeng;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To develop a novel method for fast analysis of one active component in water extracting process of Paeonia lactiflora with near infrared transmittance spectroscopy. Method: The calibration model of 54 extraction samples was developed by the spectral data pretreatment of the multiplicative scatter correction (MSC) with the spectral regions (6 101.9-5 446.2,4 601.5-4 424.1 cm-1) and analyzed the correlation between the spectra and the corresponding values by PLS method. Result: The R2was 0.998 9, the root mean square error of Cross-Validation (RMSECV) was 0.025 2 and the best dimension was six. The paeoniflorin in the 13 samples was evaluated by using the model established, root mean square errors of prediction(RMSEP)was 0.086 1. Conclusion: This method is not only rapid, simple, accurate and reliable, but also has great application prospects in the optimization of extraction technology and on-line monitoring of production process in TCM field.关键词：the near infrared transmittance spectroscopy;Paeonia Lactiflora;paeoniflorin;extracting process monitoring;multiplicative scatter correction(MSC)
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of saikosaponin A in Radix Bupleuri by combining near-infrared spectroscopy with the chemometrics method. Method: The content of saikosaponin A in Radix Bupleu was determined by HPLC as a reference method. Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method. Result: RMSECV (root-mean-square error of cross-validation)of the model for saikosaponin A was 0.321 and the correlation coefficients of the calibration model was 0.934.External validation proved that the relative coefficient of the predicted value and the HPLC value was 0.951,with measured recycle rate of 96.64%. Conclusion: NIRS can be used in the determination of saikosaponin A, which sets up the foundation of product-line control of Radix Bupleuri.关键词：NIR;Radix Bupleuri;saikosaponin A;quantitative analysis model
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发布时间：2024-01-04 - 摘要：Objective: This article analyze and compare the volatile oil of Magnolia officinalis from different regions. Method: Headspace solid phase microextraction (HS-SPME) was used to extract volatile oil of M. officinalis from different regions and gas chromatography-mass spectrometry (GC-MS) was used to analyze their compositions. The relative percentage of various volatile oil ingredients was determined by the area normalizing method. Result: Forty-seven kinds of compounds were identified in three different regions, a total of six kinds of ingredients were the same. Conclusion: The comparative analysis showes that volatile oils of M. officinalis different regions have some differences in composition and content.关键词：Magnolia officinalis;volatile oil components;gas chromatography-mass spectrometry
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发布时间：2024-01-04 - 摘要：Objective: To establish the determination method of luteolin content in Codariocalyx motorius. Method: VP-ODS Cl8(4.6 mm×250 mm, 5 μm) colmnn was used, the mobile phase was methanol-0.3% phosphonic acid solution (60∶40) with the flow rate as 1.0 mL·min-1. The detection wavelength was set at 350 nm. Result: The linear relation of hyperin was good in the range from 0.040 72 to 0.651 52 μg. The average recovery was 100.35%, RSD was 0.80%. Conclusion: The method established is accurate, sensitive and good in reproducibility. It can be applied to the quality control of C. motorius.关键词：Codariocalyx motorius;luteolin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To prove the long-circulating and distribution of liposome which is modified with choesterol-poly (ethylene glycol)2000-glycyrrhetinic acid (Chol-PEG-GA)(CPGL) in vivo and provide theoretical basis for targeting therapy of tumor. Method: The biodistribution of NIRD-15 labeled liposome was studied in mice by fluorescence animal imaging. The mice were randomly separated in conventional liposomes (LP) group and CPGL group. The fluorescence intensities in vivo and in isolated organs were measured 5,10,15,30,60,120,180,360,600 min after injection. Result: LP faded away quickly through metabolism in organs of body, transferred by circulation system, while few phenomenon of NIRD-15's enrichment in liver occurred. After injected CPGL into body, the content ratio of NIRD-15 in the body was increased slowly. The phenomenon of NIRD-15 enrichment occurred in the liver 15 min after administration, and the concentration was still higher than LP after 600 min, which suggested liposomes modified with Chol-PEG-GA can target to the liver and extend its residence time in liver. Conclusion: CPGL can control the release of the drug in vivo, prolong the biological half life, and improve the liver targeting effect.关键词：Chol-PEG-GA;liposome;NIRD-15;liver targeting;animal imaging
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发布时间：2024-01-04 - 摘要：Objective: To investigate the contents after single oral one kind of homogeneous molecular weight polysaccharide(MDG-1) extracted from Ophiopogonis Radix in rat urine and faeces by high performance gel permeation chromatography(HPGPC). Method: Labeling the MDG-1(F-MDG-1) and detecting replacement of fluorescein isothiocyanate(FITC) then the HPGPC was used to detect the contents of MDG-1 in urine and faeces after single oral administration. The rats were given F-MDG-1 at doses of 300 mg·kg-1 then urine and faeces were collected at predefined time points (0, 4, 12, 24, 48, 72 h postdose;n=six per time point) then stored at -20 ℃ to be analyzed. Result: The F-MDG-1 in rats urine was described with the max 0.808 7 mg at 12 h and a degradation model to the lowest 0.1053 mg at 72 h. The F-MDG-1 in rats faeces was described with the max 21.332 4 mg in 12 h and a degradation model to the lowest 0.506 5 mg at 72 h. Conclusion: The combination of the FITC prelabeling method with the HPGPC is confirmed and tested and feasibile.This methodology should be helpful to other polysaccharides research in rat urine and faeces. In this study, F-MDG-1 was absorbed hardly and the main excretion way is through faeces.关键词：Ophiopogonis Radix polysaccharides;homogeneous molecular weight polysaccharide;fluorescein isothiocyanate;high performance gel permeation chromatography;urine;faeces
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发布时间：2024-01-04 - 摘要：Objective: To analyze the feature of the drug pairs of Flos Lonicerae and Fructus Forsythiae by determining the content of chlorogenic acid and forsythin. Method: The content of chlorogenic acid and forsythin in different organs was analyzed by using the Diamonsil C18(4.6 mm×200 mm, 5 μm) column with acetonitrile-0.4% phosphoric acid as mobile phase; the velocity of flow was 1.0 mL·min-1, and the detection wavelength was set at 280 nm. Result: Chlorogenic acid and forsythin can be detected in all the organs and there was more in brain except the gastrointestinals. Conclusion: The distribution of chlorogenic acid and forsythin fit in with the feature'clearing away the heat-evil to relieve the exterior with light drug'.关键词：drug pairs;chlorogenic acid;frusthin;tissue distribution
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发布时间：2024-01-04 -
Comparative Study on Purgative Effects Among Different Components of Processed Rhez Radix et Rhizoma
摘要：Objective: To study the different purgative effects and molecular mechanisms among different components of processed Rhez Radix et Rhizoma (extracts from Rhez Radix et Rhizoma, cooked Rhez Radix et Rhizoma and Rhez Radix et Rhizoma carbon). Method: Mice were randomly divided into control group and treatment groups with different components of processed Rhez Radix et Rhizoma at a dose of 10 g·kg-1. The different samples were administered to mice for observing the purgative number, the first purgative time,the 5 hour purgative times and defecation quantity. The effects on small intestinal propulsion were determined by propulsion rate of charcoal powder in mice. The effects on Na+-K+-ATPase in rat colon wall cell membrane were determined after the rat was treated for 5 days. Result: The mice showed no purgative effect after be administered with different components of the processed Rhez Radix et Rhizoma except the component 2 and 3.As the total number of defecation in mice was concerned, except the cooked Rhez Radix et Rhizoma component 2 and 3 as well as the Rhez Radix et Rhizoma component 4 and Rhez Radix et Rhizoma carbon component 4, other components were significantly higher compared with the controls (P<0.05 or P<0.01). Compared with the Rhez Radix et Rhizoma component 2, the number of defecation for the cooked Rhez Radix et Rhizoma component 2 was significantly less (P<0.05). Compared with the Rhez Radix et Rhizoma component 3, the number of defecation for the cooked Rhez Radix et Rhizoma component 3 and the Rhez Radix et Rhizoma carbon component 3 was significantly less (P<0.01). Compared with control group, the Rhez Radix et Rhizoma component 2 and 3 could significantly promote the intestinal propulsion (P<0.05), while the other components also had the same tendency. Among the different components of the same procesed Rhez Radix et Rhizoma, the intestinal propulsions of the Rhez Radix et Rhizoma component 2 and 3 were higher than the cooked Rhez Radix et Rhizoma component 2 and 3 respectively (P<0.05). All the components of the processed Rhez Radix et Rhizoma could inhibit Na+-K+-ATPase in cell membrane of colon wall, among them the Rhez Radix et Rhizoma component 2 and 3 and the Rhez Radix et Rhizoma carbon component 3 showed the significant inhibitory effect compared with the control group (P<0.05 or P<0.01). Among the component 2, the highest inhibition for Na+-K+-ATPase appeared from Rhez Radix et Rhizoma, compared with the processed Rhez Radix et Rhizoma and the Rhez Radix et Rhizoma carbon (P<0.05 or P<0.01). Among the component 3, the lowest inhibition for Na+-K+-ATPase appeared from Rhez Radix et Rhizoma, with significance between that of the processed Rhez Radix et Rhizoma (P<0.05). Conclusion: The Rhez Radix et Rhizoma component 2 and 3 possess the strongest purgative effects and the inhibitory action on Na+-K+-ATPase is potentially the molecular mechanism.关键词：processed Rhez Radix et Rhizoma;different components;purgative effect;number of defecation;small intestinal propulsion rate- 2
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发布时间：2024-01-04 - 摘要：Objective: Our aim was to investigate the protein changes of the expression of γ-aminobutyric acid B2(GABABR2) receptor in primary cultured rat hippocampus neurons, to explore the intervention mechanism of Shuyu capsule for treating depression emotion preliminarily. Method: We used the chronic mild stress(CMS)to establish the depression emotion(DE) model in rats,and then the rats were evaluated by sucrose intake,the body weight and open-field from beginning to the end for the model. Serum of rats in each group was collected and then added into the medium of vitro cultured hippocampus primary neurons. At last the expression of γ-aminobutyric acid B2 receptor was detected with Western blot respectively. Result: Compared with the normal group, the weight, the total score of open field test and the sucrose intake of preference test of rats in depression emotion model group lessened significantly (P<0.01). On the other hand, compared with the depression emotion model group, the weight, the total score of open field test and the sucrose intake of preference test of rats in Shuyu capsule,bupleurum and fluoxetine treatment groups increased significantly(P<0.05 or P<0.01). Western blot demonstrated that the serum of model group rats obviously increased the protein levels of GABABR2 (P<0.01), compared with the normal control group. While serum of the Shuyu capsule and fluoxetine treatment groups markedly decreased the protein levels of GABABR2 (P<0.05), the bupleurum group had no difference with the model group, but the trendhad improved. Conclusion: The serum of rats with depression emotion could induce the high expression of GABABR2, the Shuyu capsule may play antidepressants by reducing the expression of GABABR2 in primary cultured rat hippocampus neurons. The bupleurum also has a good antidepressant effect, but the effect is less than Shuyu capsule.关键词：depression emotion;Shuyu capsule;γ-aminobutyric acid B2 receptor;serum pharmacology
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of Fufang Danshenfang and total saponin of Dioscorea compatibility on myocardial ischemia reperfusion injury in rats. Method: Seventy SD rats were randomly divided into 10 groups: sham-operation group, model group, Salvia extract (300 mg·kg-1), total saponins of panax notoginseng (PNS) 100 mg·kg-1, dioscorea saponins 100 mg·kg-1, compatibility (Salvia extract+PNS+borneol+Dioscorea saponins) three dose of (300+100+10+50),(300+100+10+100),(300+100+10+200) mg·kg-1, compound Danshen tablets 300 mg·kg-1,Tongxinluo capsules 300 mg·kg-1. Each group was ig administrted for 7 consecutive days.The model of myocardial ischemia reperfusion injury in rats was induced by ligation of the left anterior descending of the coronary artery(LAD) after one hour of end administration. Serum superoxide dismutase (SOD), lactic clehydrogense(LDH) and creatine kinase isoenzyme(CK-MB) activity were determined by kit, the TTC staining method was used to determine the myocardial infarct size, TUNEL staining was used to detect apoptosis. Result: Compatibility (300+100+10+100) mg·kg-1 could significantly enhance SOD activity (P<0.01) significantly reduce myocardial LDH and CK-MB activity (P<0.01); compared with the ingredients and the original side, infarct size of 20.78% was significantly lower than the Salvia extract 34.04%(P<0.05), PNS 34.62% (P<0.05), the total saponin of Dioscorea 36.19% (P<0.05), Fufang Danshen Tablets 26.47%; apoptosis rate of 16.53%, 25.08% (P<0.05) was significantly lower than the Salvia extract 25.08%, PNS 22.12% (P<0.05), the total saponin of Dioscorea 24.45% (P<0.05), Fufang Danshen Tablets 18.14%. Conclusion: Fufang Danshenfang and the total saponin of Dioscorea compatibility have showed protective effects on myocardial ischemia reperfusion injury, which is much better than other compostion and Fufang Danshenfang.关键词：Fufang Danshenfang;total saponin of Dioscorea;myocardial ischemia and reperfusion;ischemic area;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To observe different extracts from Santalum album on isolated smooth muscle contraction. Method: Using BL-420F biological function experimental system and the HW-400S constant temperature smooth muscle bath isolated smooth muscle contraction was observed. Result: Water extraction liquid ethyl ether extract of S. album, water extraction liquid ethyl acetate extract of S. album, ethanol-extraction liquid ethyl ether extract of S. album, ethanol-extraction liquid ethyl acetate extract of S. album had inhibitory effect (P<0.01).Water extraction liquid ethyl ether extract of S. album, water extraction liquid ethyl acetate extract of S. album, ethanol-extraction liquid petroleum ether extract of S. album, ethanol-extraction liquid ethyl ether extract of S. album, ethanol-extraction liquid ethyl acetate extract of S. album from had a antagonist function (P<0.01). Water extraction liquid ethyl acetate extract of S. album, have obvious synergy on the flabby condition caused by atropine (P<0.01). Conclusion: Different extracts of S. album possess the inhibition on isolated intestinal smooth muscle.关键词：Santalum album;extract;Guinea pigs vitro bowel;acetylcholine;atropine
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发布时间：2024-01-04 - 摘要：Objective: To study the influences of Weiweikang granule(WWKG) on the expression of proliferating cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR) of gastric mucosa with chronic atrophic gastritis(CAG), and evaluate the possible mechanisms. Method: Ninety SD rats were randomly divided into five groups. The high dose WWKG group, low dose WWKG group, Sanjiu Weitai granule(SJWTG) group and model group.The multiple stimulations were used to duplicate the CAG model. After modeled successfully, the high dose WWKG group, low dose WWKG group, SJWTG group and model group were intragastrically injected(ig) with WWKG at dose of 6, 3 g·kg-1, and SJWTG at dose of 1.6 g·kg-1, respectively and normal saline(0.9%)at dose of 20 mL·kg-1. After 30 days, the rats'stomach mucous membrane atrophy situation was observed with the help of light microscopy and transmission electron microscopy and the expression of PCNA and EGFR were observed with immunohistochemical method. Result: The light microscopy and electron microscopy examination showed that the pathological changes of gastric mucosa in large dose WWKG group and small dose WWKG group were significantly milder than those in the model group. Compared with the control group, the expression of PCNA labeling index(LI 44.96%)and EGFR(average obsorbance,A0.183 ±0.030) in the model group was higher than the normal group (LI 29.30%, A 0.125±0.039)obviously(P<0.01).Compared with the model group, the expression of PCNA and EGFR in large dose WWKG group(LI 29.58%,A 0.100±0.023)and small dose WWKG group (LI 29.97%, A 0.104±0.027)was significantly lower(P<0.05 or P<0.01). Conclusion: WWKG can significantly repair gastric mucosa of rats with CAG and its mechanism may be actualized by decreasing the expression of PCNA and EGFR and restraining cell proliferation and malignant transformation.关键词：chronic atrophic gastritis;Weiweikang granul;PCNA;EGFR
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发布时间：2024-01-04 - 摘要：Objective: To observe apoptosis of nonalcoholic fatty liver in rat liver tissue and discuss mechanism of Qutan Huoxue decoction using non-alcoholic fatty liver rat model. Method: Sixty SD male rats were randomly divided into 2 groups:normal control group with 10 rats, which was given normal diet; model group with 50 rats, which was given high fat diet. The model group. After second week, 50 rats were divided into model control group, low dose group (Qutan Huoxue decoction 4.5 g·kg-1), middle dose group (Qutan Huoxue decoction 9 g·kg-1), high dose group (Qutan Huoxue decoction 13 g·kg-1) and Kaixilai drug group (30 mg·kg-1). Model control group and normal control group were given the same dose of NS. According to above drug plan, at the end of 8,9,10th week, 1-2 rats were dissected from the model group to observe pathological changes in liver tissue and blood biochemical parameters alanine aminotransferase(ALT), aspartate aminotransferase(AST), triglycerides(TG), total cholesterol(TC) and so on. All rats were dissected at the end of 10th week to observe hepatic function,blood lipid and apoptosis. Result: In model control group compared with normal control group, serum ALT, AST, TC, TG level,range of hepatic pathological inflammation, index in hepatic apoptosis showed significantly increased (P<0.01); low, middle, high dose treatment groups of Qutan Huoxue decoction, Kaixilai treatment group compared with model group, their indexes were significantly decreased (P<0.05). Conclusion: Qutan Huoxue decoction can significantly reduce NAFL liver fatty degeneration,regulate lipid metabolism, improve liver function and decrease the apoptosis in liver.关键词：non alcoholic fatty liver;Qutan Huoxue decoction;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: Through the establishment of the model of oxidatie stress damage induced by H2O2 in myocardium cells, to observe the protective effects of total saponin from Panax japonicus (SPJ) on myocardial cells. Method: Neonatal rat cardiomyocytes were randomly divided into four groups: normal, model (H2O2), H2O2+l-SPJ (SPJ, 50 mg·L-1) and H2O2+h-SPJ (SPJ, 100 mg·L-1). H2O2was used to induce oxidative stress injury in primary cultured cardiomyocytes of neonatal rats for 2 hours, and then incubated 24 hours with SPJ (50, 100 mg·L-1). The beating rates of cardiomyocytes were monitored under inverted microscope, cell viability was detected by MTT assay. The content of lactate dehydrogenase (LDH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in culture medium were detected after treated by SPJ with colorimetric technique at 24 hours. Result: SPJ could significantly increase beating rates of cardiomyocytes; and the contents of LDH, MDA in culture medium were remarkably decreased, and activities of SOD, CAT and GSH-Px were significantly increased (P<0.05 or P<0.01). Conclusion: SPJ exerted protective effects on oxidative stress injury induced by H2O2 in cardiomyocytes of neonatal rats.关键词：total saponin from Panax japonicus C. A. Mey;H2O2;cardiomyocytes;oxidative stress;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To observe the impact about moxa flavone on animal experimental acne and pathology links. Method: To observe the influences of 60, 30 mg·kg-1 moxa flavone on models of experimental rabbit ear keratinization, the influences of 60, 30, 15 mg·kg-1 moxa flavone on the models of the rat voix pedis engorgement and rats blood stasis syndrome. The influences of 100, 50, 25 mg·kg-1 moxa flavone on the models of the mice ear engorgement and the delayed hypersensitivity were evaluated. The treatment of moxa flavone on acne was evaluated. Result: 60 mg·kg-1 of moxa flavone can significantly reduce the level of ear keratinization of rabbit models and restrain obviously voix pedis engorgement of the rat models after 5 h medication(P<0.01);60 mg·kg-1 of moxa flavone has obviously improved the blood viscosity of rat acute in blood stasis model(P<0.01 and P<0.05); 100 mg·kg-1 of moxa flavone can significantly depress the ear engorgement of the mouse models (P<0.05) and the increasing CD4+ and CD8+ caused by the delayed hypersensitivity, and inhibit obviously the indexes of immune organs(P<0.05). Conclusion: The experiments show that moxa flavone can cure acne through the experimental keratinization, the blood circulation activating, stasis eliminating, antiinflammatory action, immunosuppression and androgen depreciation.
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发布时间：2024-01-04 - 摘要：Objective: To evaluate anti-fatigue and hypoxia tolerance effect of mogrosides in mice. Method: Mice were randomly divvied into normal control group, model control group, positive control group (Hongjingtian capsule, 570 mg·kg-1), mogrosides high dosage (300 mg·kg-1), medium dosage (150 mg·kg-1) and low dosage groups (75 mg·kg-1). After orally administrated for 21 days consecutively, exhaustive swimming test and atmospheric hypoxia test were employed to evaluate anti-fatigue and hypoxia tolerance effect of mogrosides in mice. Furthermore, liver glycogen and muscle glycogen level were examined, urea nitrogen level, lactic acid level and lactate dehydrogenase activity after swim were determined. Result: Mogrosides significantly extended swimming time (P<0.05), and extended hypoxia survival time at 75 mg·kg-1 and 150 mg·kg-1 (P<0.05). Moreover, liver glycogen and muscle glycogen level were significantly increased after treatment (P<0.001), urea nitrogen level, lactic acid level were decreased (P<0.001), lactate dehydrogenase activity was enhanced (P<0.05). Conclusion: Mogrosides possesses abilities of anti-fatigue and hypoxia tolerance. The effects could be mediated by increased glycogen supplying and improved lactate metabolism.关键词：mogrosides;anti-fatigue;exhaustive swimming test;hypoxia tolerance
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发布时间：2024-01-04 - 摘要：Objective: To explore the effects of Mayinglong eye cream on infraorbital dark circles. Method: The right rear toes of SD-rats were hypodermic injected with carrageenan and then coated with Mayinglong eye cream in different quality percentage (2%, 1%, 0.5%) to observe toe swell at different times and toe weight before and after drug administration. Anti-inflammatory effect of Mayinglong eye cream was evaluated. The blood flow of auricle of SD-rats was monitored by Peri Cam PSI system. The effect of Mayinglong eye cream on regional blood flow of auricle skin surface was observed. Result: Mayinglong eye cream showed effects of anti-inflammation and reduced regional blood flow. Conclusion: Mayinglong eye cream could improve infraorbital dark circles via the effect of reducing blood flow and anti-inflammation.关键词：infraorbital dark circles;anti-inflammation;blood flow
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of the extract of Angelica sinensis on the melanin synthesis in co-culture system of human melanoma and keratinocytes cells. Method: A375 and HaCaT cell were cultured respectively in MEM and co-cultured in 1∶2 ratio for 24 hours. The co-cultured cells were cultured for 48 hours after MEM containing different concentration of A. sinensis were added to the dishes. The proliferation, the tyrosinase(TYR) activity and melanin contents of the co-culture cells were measured by MTT,dopa-oxidase and NaON assays respectively. Result: Compared with control group,the cells proliferation were markedly increased by the extract of A. sinensis in the concentration range of 0.5-2 g·L-1(P＜0.01),the melanin synthesis were markedly suppressed in the concentration range of 0.25-2 g·L-1(P＜0.05 or P＜0.01),TYR activity were significantly suppressed in the concentration range of 0.125-2 g·L-1(P＜0.01). Conclusion: The water extract of A. sinensis can decrease the melanogenesis by decreasing the activity of tyrosinase though it can increase the proliferation of the cells in higher concentration.关键词：Angelica sinensis;melanoma;keratinocytes;co-culture;melanin;tyrosinase
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Compound Ginseng Brain Tonic Liquid (CGBTL) on cerebral ischemia-reperfusion injury. Method: Rats were randomly divided into normal control group, cerebral ischemia and reperfusion model group, large drug dose + model group (CGBTL 10 g·kg-1), small drug dose + model group (CGBTL 5 g·kg-1), vitamin E + model group. The model of cerebral ischemia reperfusion rats was established by ligaturing carotid. The lipid peroxide content of brain cell was measured with thiobarbituric acid. Reduced glutathione was determined by the method of spectrophotometer. Oxygen consumption was measured with clark oxygen electrode method. Result: In rats with cerebral ischemia-reperfusion injury, there showed an increase in lipid peroxide content (P <0.001), a decrease in reduced glutathione (P<0.001) and an increase in oxygen consumption (P<0.001) compared with those in the normal control group. In CGBTL large dose and small dose groups, compared with the model group, there showed a decrease in lipid peroxide content (P <0.001), and an increase in reduced glutathione content (P <0.001) and a decrease in oxygen consumption (P<0.001). In vitamin E treatment group, the outcomes were significant (P <0.001). Conclusion: CGBTL has preventive effect on cerebral ischemia-reperfusion injury.关键词：Compound Ginseng Brain Tonic Liquid;cerebral ischemia-reperfusion;lipid peroxidation;reduced glutathione;oxygen consumption
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of the water extract of Tetrastigmatis hemsleyani and the serum containing it on the proliferation in vitro. Method: The mice of clean grade were randomly assigned into 5 groups: high dose group (20 g·kg-1), moderate dose group(10 g·kg-1), low dose groups (5 g·kg-1), positive control cyclophosphamide (CTX,5 g·kg-1) group and negative control group(distilled water). Every group was administered by intragastric administration 20 mL·kg-1 each, twice a day within 3 successive days. One hour after the last administration on third day, blood was collected from every decapitated mouse. The blood was centrifugalized for 20 min at 3 000 r·min-1after standing at 4 ℃. The separated serum was filtered to remove bacteria by 0.22 μm sepatate film at 56 ℃ after inactivated for 30 min and stored at -20 ℃ for later use. The suspensions of AGS cells in logarithmic growth phase digested by 0.25% tripsin were inoculated in 96 well culture plate with a cell density of 2.5×104/L. The different concentrations of the water extract of T. hemsleyani and the serum containing it were added into the culture after 24 h culture. MTT assay was used with determinating A value in the wavelength of 570 nm to evaluate the cell proliferation of the water extract of T. hemsleyani and the serum containing it for 48 h. Result: When the concentration of the water extract of T. hemsleyani was range from 1 g·L-1 to 40 g·L-1, a significant proliferation inhibiting (P<0.01) was shown and its 50% inhibition concentration(IC50) was 13.15 g·L-1. High, moderate and low dose treatment groups (20, 10, 5 g·kg-1) also significantly inhibited the proliferation of AGS cells and its IC50was 70.06 g·L-1. Both of the proliferation inhibitings were shown in dose-dependent manner. Conclusion: The water extract of T. hemsleyani can strongly inhibit the AGS cells proliferation.关键词：Tetrastigmatis hemsleyani;AGS cells;the serum containing T. hemsleyani;inhibition of proliferation in vitro
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发布时间：2024-01-04 - 摘要：Objective: To explore intervention of Jiawei Gualou Qumai Tang(GLQM)in hepatocyte growth factor, insulin-like growth factor-1 and vascular endothelial growth factor in rats with diabetic nephropathy. Method: Except those in the normal group, all rats of diabetic nephropathy model were established by with unilateral nephrectomy and intraperitoneal injection of streptozotocin(STZ) induced production. Then rats were randomly divided into five groups: the model group, and the four treated groups treated with low dose of GLQM-L, medium dose of GLQM-M, high dose of GLQM-H, positive medicine (Captopril tablets). The treatments were given via gastrogavage every day starting from the 4th week of modeling, groups treated with high, medium and low dose of GLQM were given via gastrogavage to 5.6, 2.8, 1.4 g·kg-1, positive drugs (Captopril tablets) group were given via gastrogavage to 1.8×10-3 g·kg-1, the normal group and model group were given 2.8 mL·kg-1 distilled water,once a day for 8 weeks. Then observed the signs of rats and the hepatocyte growth factor (HGF), insulin-like growth factor-1(IGF-1) and vascular endothelial growth factor (VEGF) changes in renal tissue of rats. Result: The HGF, IGF-1 and VEGF in renal tissues of rats with model were higher than the normal group, there were significant difference among the model group and the normal group(P<0.01); the HGF in renal tissues of rats with GLQM-M and IGF-1 in renal tissues of rats with GLQM-H and GLQM-M and VEGF in renal tissues of rats with GLQM-H and HGF and IGF-1 and VEGF in renal tissues of rats with Captopril tablets were lower than the model group, there were significant difference among the treated groups and the model group(P<0.01); the HGF in renal tissues of rats with GLQM-M and VEGF in renal tissues of rats with GLQM-H were same as Captopril tablets group, there were no significant difference among them; the IGF-1 in renal tissues of rats with GLQM-H was lower than the GLQM-M,there was significant difference between two(P<0.01). Conclusion: Jiawei Gualou Qumai Tang could decrease the HGF, IGF-1 and VEGF in renal tissues of rats, improve microcirculation, increase supply of blood oxygen to renal, speed up the repair and regenerate of injury renal tissue.关键词：diabetic nephropathy;Jiawei Gualou Qumai Tang;hepatocyte growth factor;insulin-like growth factor-1;vascular endothelial growth factor
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of traditional Chinese medicine Liuyueqing polysaccharide(LYQP) on duck liver injury induced by duck hepatitis B virus(DHBV),and provide an experimental base for research and developing a new durg for anti-HBV. Method: Liver injury induced by DHBV in Guangxi brown spotted ducks was used as animal model. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), DHBsAg and DHBeAg in serum were detected before treatment(T0), 7 days(T7), 14 days(T14)after treatment, and 3 days after stopping treatment.The extent of liver injury was observed by HE staining. Result: Compared with modle group, the serum levels of ALT, AST, DHBsAg and DHBeAg were decreased significantly (P<0.05 or P<0.01) and at 3 days after stopping treatment, the ALT, AST, DHBsAg and DHBeAg in serum in high-dose and middle-dose did not return. HE staining showed that LYQP had significant protective effects against the duck liver injury induced by DHBV. Conclusion: LYQP has a protective effect against the duck liver injury induced by DHBV.关键词：Liuyueqing polysaccharide;DHBV;ALT;AST;DHBsAg;DHBeAg
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Astragali Radix and Angelicae Sinensis Radix on level of interferon-γ (IFN-γ) and interleukin-4(IL-4) in serum and expression of signal transducers and activators of transcription 6(STAT6), signal transducers and activators of transcription 4(STAT4) of airway allergic inflammation in rats, in order to partly show the mechanism of Astragali Radix and Angelicae Sinensis Radix in treating airway allergic inflammation. Method: The model of airway allergic inflammation in rats were caused by OVA. Astragali Radix and Angelicae Sinensis Radix were used to control the model rats. The contents of IFN-γ and IL-4 in the serum were detected by ELISA. The expression of STAT6, STAT4 in lung tissue were detected by Western blot. Result: The level of IL-4 in serum of model group were significantly higher than those in the control group. The level of IFN-γ in serum of model group were significantly lower than those inthe control group. Astragali Radix can depress the level of IL-4 in serum, and increase the level of IFN-γ in serum. Angelica can depress the level of IL-4 in serum. Compatibility of Astragali Radix and Angelicae Sinensis Radix can depress the level of IL-4 in serum, and increase the level of IFN-γ in serum. Astragali Radix can depress the expression of STAT6, p-STAT6 in lung tissue, and increase the expression of p-STAT4.Angelicae Sinensis Radix can depress the expression of p-STAT6.Compatibility of Astragali Radix and Angelicae Sinensis Radix can depress the expression of STAT6,p-STAT6 in lung tissue, and increase the expression of STAT4,p-STAT4. Conclusion: Compatibility of Astragali Radix and Angelicae Sinensis Radix may adjust the balance of Th1 and Th2 by restaining STAT6 and promoting STAT4 to control the airway allergic inflammation.关键词：Astragali Radix;Angelicae Sinensis Radix;airway allergic inflammation;IFN-γ;IL-4;STAT6;STAT4
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Dongyao Wuwei Zhixie decoction on serum levels of interleukin-1(IL-1)and interleukin-10(IL-10) in rats with intestinal ulcerative colitis and. Method: Ulcerative colitis rat model was copied by 2,4,6-TNBS. Sixty SD rats were randomly divided into normal group,model group,Dongyao Wuwei Zhixie decoction low,middle and high dose group(5.5,11,22 g·kg-1),Mesalazie group (0.5 g·kg-1),10 in each group. The general situation and histopathological changes were observed.The ultrastructural changes of UC model rats were investigated by transmission electron microscope and the rat serum levels of IL-1,IL-10 were detected by ELISA 10 days later. Result: Dongyao Wuwei Zhixie decoction effectively improved the serum levels of IL-10, reduced the serum levels of IL-1, and had significant effect of repairing the intestinal mucosal injury. Conclusion: Dongyao Wuwei Zhixie decoction achieves a therapeutic effect by inhibiting the production of proinflammatory cytokines IL-1 and improving the production of antiinflammatory cytokines IL-10.关键词：Dongyao Wuwei Zhixie decoction;ulcerative colitis;histopathological changes;interleukin-1;interleukin-10
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发布时间：2024-01-04 - 摘要：Objective: To study the correlation of plasma concentration before and after ultrafine grinding and Corydalis Decumbentis Rhizoma bioavailability by oral administration in mice. Method: Acetic acid induced writhing response was used to observe analgesic effect in mice and the blood contained in the amount of alkaloids. Result: By ultrafine grinding of Corydalis Decumbentis Rhizoma, stronger inhibitory effect on acetic acid induced writhing response was fund compared with the ordinary pieces, alkaloid content in the blood was higher than ordinary pieces. Conclusion: Ultrafine grinding can improve the bioavailability of Corydalis Decumbentis Rhizoma.关键词：ordinary pieces of Corydalis Decumbentis Rhizoma;ultrafine powder of Corydalis Decumbentis Rhizoma;alkaloid;analgesic
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发布时间：2024-01-04 - 摘要：Objective: To explore the anti-fatigue effect and mechanism of polysaccharides from mulberry amylose(MA). Method: A total of 224 Kunming mice were randomly divided into 4 batches,each of which consisted of 4 groups:MA groups at the dosages of low(100 mg·kg-1),middle(300 mg·kg-1)and high(900 mg·kg-1),and the control group. Each mouse was administered once a day for 35 days. After the end of the administration,all the mice were evaluated for their weight-loaded swimming duration and the contents of hepatic glycongen, blood urea nitrogen(BUN) and blood lactic acid. Result: MA at the high dosages could prolong weight-loaded swimming duration(31.3±15.13) min,compared with (7.15±2.91) min. In addition, an increase of hepatic glycongen (26.23±6.08) mg·g-1,compared with (18.75±3.12) mg·g-1 and a decline of BUN (9.66±1.35) mmol·L-1,compared with (12.07±1.64) mmol·L-1 were observed in mince administered MA at the high dosages. Conclusion: MA could alleviate physical fatigue.关键词：mulberry amylose;anti-fatigue effect;mechanism
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Zhishi Xiaopi Pills combined with western medicine on Fas protein expression in gastric epithelial cells in rats with Helicobacter pylori infection and to explore the related mechanism. Method: BALB/c mice were fed with NaHCO3 solution of 5% and Helicobacter pylori(Hp) strains SS1 international standards to establish Hp infection, and randomly divided into three groups: the Chinese medicine group, the western medicine group, and the Chinese medicine combined with western medicine group. The injury of gastric mucous membrane, cell apoptosis index and epithelial cell expression of Fas were investigated by HE staining, Tunel method and immunohistochemical evaluation (SABC). Result: In three groups, the over-expression of epithelial Fas protein could be inhibited by the interventions. The model group expression was (28.30±2.36)%, while the Chinese medicine combined with western medicine group, the western medicine group and the Chinese medicine group were (15.50±2.39)%, (22.0±1.90)%, (25.9±2.34)% accordingly. The inhibition degree appeared to be the Chinese medicine combined with western medicine group>the western medicine group>the Chinese medicine group. In the three groups, the injury of gastric mucous membrane was improved to different degree. The Chinese medicine combined with western medicine group showed less injury (lesion index LI=1.10±0.18) compared with the western medicine group(1.30±0.26) and the Chinese medicine group (1.80±0.25). As cell apoptosis was concerned, the apoptosis index in the Chinese medicine combined with western medicine group (5.10±0.67)% was smaller than those in the western medicine group (8.70±0.88)% and the Chinese medicine group (11.60±1.11)%. The model showed an index of (16.60±1.74)%. Conclusion: Zhishi Xiaopi Pills combined with western medicine can improve Helicobacter pylori-induced gastric injury. The mechanism may be related to inhibition of cell apoptosis and inhibition of expressions of Fas protein in gastric epithelial cells.关键词：helicobacter pylori;animal model;Zhishi Xiaopi Pills;western medicine;Fas protein
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-hypertentive and sedative effects of Tianma Gouteng Jiangya capsules (TGJC). Method: Spontaneous hypertention rats were orally given TGJC (5.4, 10.8, 21.6 g·kg-1) daily for 4 weeks. Anti-hypertentive effect was evaluated. Mice were orally given TGJC (7.8, 15.6, 31.2 g·kg-1) daily for 5 days. One hour after the last administration, intraperitoneal injection of sodium pentobarbital 55 mg·kg-1 was carried out, and sleep time was recorded. Similarly, intraperitoneal injection of thio-semicarbazide (16 mg·kg-1) was carried out, the time of convulsions and the incubation period were recorded. In addition, one hour after the last administration, mice were placed in YLS-1A versatile recorder to record the number of locomotor activities in mice within 10 min. Result: Three doses of TGJC could significantly reduce the blood pressure in the hypertensive rats (P<0.01). TGJC high dose could significantly prolong the sodium pentobarbital induced sleep time P<0.05), extend the thio-semicarbazide-induced seizure latency (P<0.05), without significant effect on the number of seizures. TGJC had no effect on locomotor activity in mice. Conclusion: TGJC has some anti-hypertention and sedative effects.关键词：Tianma Gouteng Jiangya capsule;hypnotic;sedative;locomotor activity
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发布时间：2024-01-04 -
Effect of Pollen Typhae Flavonoids on Hemorheological Parameters and Platelet Aggregation in Rabbits
摘要：Objective: To study the effect of pollen typhae flavonoids on hemorheological parameters and platelet aggregation. Method: Forty-eight female rabbits were divided into normal group (saline), model group (saline), Salvia miltiorrhiza group (tanshinone 0.13 g·kg-1), pollen typhae flavonoids high, median, low dose group (dose of 0.8, 0.5, 0.2 g·kg-1). Except for the normal group which was given sc saline, other groups were given sc adrenaline hydrochloride and socked in ice-water, so as to establish acute blood stasis model. Then the effect of pollen typhae flavonoids on hemorheological parameters was studied by this model. Based on the platelet aggregation in rabbits, the inhibitory action of pollen typhae flavonoids on the formation of thrombosis was studied. Result: Pollen typhae flavonoids could significantly reduce viscosities at each shear rates and HCT for blood stasis model, there were significant differences (P＜0.05 or P＜0.01), and could significantly reduce the maximum platelet aggregation rate and shorten the maximum agglutinating time with significant differences (P＜0.01). Conclusion: Pollen typhae flavonoids can obviously ameliorate the hemorheological parameters and inhibit the platelet aggregation.关键词：pollen typhae flavonoids;hemorheology;platelet aggregation- 2
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Sanqing Jiangtang Fang on α-glucosidase activity. Method: α-glucosidase from small intestine of rats was extracted and the content was determined. The optimal reaction system was set by orthogonal experiment. Sanqing Jiangtang Fang was extracted with water and ethanol respectively and prepared into solutions at 25, 2.5,0.25,0.025,0.002 5 g·L-1. Acarbose was used as the positive drug. The content of glucose was measured by glucose oxidase method to evaluate the inhibitory effect on α-glucosidase. The inhibitory rate was calculated. Result: Water-extraction preparation and ethanol-extraction preparation of Sanqing Jiangtang Fang could inhibit α-glucosidase activity, and showed marked concentration-effect relationship. The inhibitory effect of water-extraction preparation at 25 g·L-1 was 64.85%±10.5%, which was in correspondence to acarbose at 2.5 g·L-1. Conclusion: Sanqing Jiangtang Fang can inhibit α-glucosidase activity.关键词：Sanqing Jiangtang Fang;water-extraction preparation;ethanol-extraction preparation;α-glucosidase
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发布时间：2024-01-04 - 摘要：Objective: To investigate apoptosis induction effects of oridonin(Ori)on human kidney carcinoma cells and its mechanisms in vitro. Method: The MTT assay was used to measure the inhibitory effects of oridonin at the concentration of 1×104/mL on A-704 cells.The apoptosis rate of A-704 cells which were impacted by Ori for 24 hours was examined by Annexin V-FITC/PI staining of Flow cytometry with the increase of the concentration, the number of apoptotic cells and necrotic cells were increased, the higher the concentration, the more the ratio of necrotic cells increased expression of Bax,Caspase-3 and Bcl-2 of A-704 cells which were impacted by 32 μmol·L-1oridonin for 24 hours were evaluated by Real-time PCR. Result: Ori could inhibit the growth of A-704 cells significantly in a time-dependent and dose-dependent manner. The inhibition rate of A-704 cells which were impacted by 64 μmol·L-1oridonin for 24 hours could be reach 73%.With the concentration of Ori increased,the number of apoptotic and necrosis cells both increased. The higher concentration, the more rate of necrotic cells increased. The ratio of necrosis cells of A-704 cells which were impacted by 64 μmol·L-1Ori for 24 hours could be reach 32.4%.Along with the extention of time, Bax and Caspase-3 expressions were up-regulated,Bcl-2 expression was down-regulated (all P<0.05). Conclusion: Ori can inhibit A-704 cells growth by induction of apoptosis in cells via activation of Caspase-3 as well as up-regulation of Bax and down-regulation of Bcl-2 gene expression.关键词：Oridonin;kidney carcinoma;apoptosis;Bax;Caspase-3;Bcl-2
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Jiajian Bufei decoction on plasma endothelin(ET) and nitric oxide(NO) in rats which have chronic obstructive pulmonary disease(COPD) and traditional Chinese medicine(TCM) syndrome such as 'Lung deficiency'. Method: The rats were divided randomly into groups and were given the medicine. Blood was detected for the contents of plasma endothelin-1(ET) and nitric oxide(NO) and comparisons were made between the groups. Result: The NO level in the large dosage group was markedly higher than that in the pathology control group. ET level in all treatment groups were both markedly lower than that in the pathology control group. Conclusion: Jiajian Bufei decoction can raise the NO and reducing the content of ET in blood plasma. Jiajian Bufei decoction can regulate the balance of ET and NO,thus prent and treat COPD.关键词：Jiajian Bufei decoction;lung deficiency;COPD;endothelin-1;nitric oxide
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发布时间：2024-01-04 - 摘要：Objective: To compare the sensitization effect of old Shengmai injection and new Shengmai injection on Beagle dogs. Method: Beagle dogs were randomly divided into 3 groups, 3 dogs in each group. Each group was injected with 5% glucose injection, old Shengmai injection and new Shengmai injection. The changes of each dog were observed from injection before and after injection until 24 hours, and the response level were determined according to the severity of the symptoms. Blood samples were collected before injection and at 10 min after injection for measuring histamine content in plasma by ELISA. Sensitization of the injection was comprehensively determined by combined the response level of symptoms and the histamine level. Result: The old Shengmai injection led to beagle dogs showing typical symptoms of anaphylactoid reactions and elevating serum histamine times.The old Shengmai injection caused Beagle dogs anaphylactoid reactionis was positive. The new Shengmai injection led to Beagle dogs showing untypical symptoms of anaphylactoid reactions and elevating serum histamine times.It caused Beagle dogs anaphylactoid reaction was suspicious. Conclusion: The old Shengmai injection can induce typical anaphylactoid reactions on beagle dogs, and the sensitization is strong. Compared with the old Shengmai injection, the symptoms of anaphylactoid reactions induced by the new Shengmai injection appeared later and showed lesser degree. The sensitization to beagle dogs can bereduced.关键词：Shengmai Injection;Beagle dogs;anaphylactoid reactions
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发布时间：2024-01-04 - 摘要：Objective: This article is to analyze the compatible mechanism of Astragalus Membranaceus and Atractylodes Macrocephala and the clinical application, which could offer some suggestions to highlight the importance of compatibility of medicines in clinial. Method: Astragalus Membranaceus and Atractylodes Macrocephala are both traditional Chinese medicine in common clinical use. Based on the compatible mechanism,combination characters and the modern relative research, we had an analysis of the compatible mechanism and the clinical sense of Astragalus Membranaceus and Atractylodes Macrocephala,which is helpful for us to correctly comprehend the clinical application and principles of the compatibility between Astragalus Membranaceus and Atractylodes Macrocephala. Result: The compatibility of Astragalus Membranaceus and Atractylodes Macrocephala is put to the clinical use mainly in three aspects such as tonifying Qi and spleen, alleviating edema and strengthening the defensive energy to firm the skin. Conclusion: The compatible mechanism of Astragalus Membranaceus and Atractylodes Macrocephala is flexible and has very wide clinical use, which offer some new ideas in clinical.关键词：astragalus membranaceus;Atractylodes Macrocephala;compatibility;analyzing
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect and the safety of Wenshen Kaiyu Tongbi Tang (WSKYTBT) on chest stuffiness and pain with heart-kidney yang deficiency. Method: Using prospective method, 78 patients with chest stuffiness and pain with heart-kidney yang deficiency were divided into two groups randomly. The treatment group of 41 patients was given modified self-designed WSKYTBT, and the control group of 37 patients was given Chinese herbs of Huoxue Huayu. The period of treatment was 4 weeks. Items such as symptoms, angina attack, electrocardiogram, cardiac function, adverse reactions were examined before treatment and after treatment. Result: In the treatment group significant efficiency rate and total efficacy rate of clinical symptom of angina pectoris was 68.3%,97.6%, in the control group they were 45.9%, 75.7% (P<0.05). In the treatment group significant efficiency rate and total efficacy rate of ECG were 63.4%, 95.1%, while in the control group they were 37.8% and 73.0% (P<0.05). On the aspect of the effect on syndrome, significant efficiency rate and total efficacy rate were 68.3% and 97.6% in the treatment group, whle 45.9% and 75.7% in the control group (P<0.05). Conclusion: WSKYTBT can effectively improve the clinical symptoms of the patients with chest stuffiness and pain with heart-kidney yang deficiency, and it is safe and reliable as well.关键词：Wenshen Kaiyu Tongbi Tang;chest stuffiness and pain with heart-kidney yang deficiency;clinical observation
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发布时间：2024-01-04 - 摘要：Objective: To observer the therapeutic effect of Gegen Qinlian Wutan decoction on tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-8(IL-8) in patients with active ulcerative colitis (UC). Method: Sixty cases with ulcerative colitis were divided into the treatment group and the control randomly. The treatment group was given Gegen Qinlian Wutan decoction by oral administration. The control group was given Sulfasalazine enteric-coated tablets. TNF-α, IL-6 and IL-8 in serum were detected by ELISA for all patients before and after treatment, and compared with 30 healthy volunteers. Result: Creative effects in the treatment was obviously better than in the control group, the difference was significant (P＜0.05). After treatment, compared with the control group, the treatment group had the lower plasma levels of TNF-α, IL-6 and IL-8 (P＜0.05). Conclusion: Gegen Qinlian Wutan decoction has obvious effect on ulcerative colitis. The down regulation of the expressions of TNF-α, IL-6 and IL-8 in serum may be one of the therapeutic mechanism.关键词：Gegen Qinlian Wutan decoction;ulcerative colitis;tumor necrosis factor-α;interleukin-6;interleukin-8
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发布时间：2024-01-04 - 摘要：Objective: To observe the influence of Shexiang Baoxin Pills (SBP) on the plasma n-terminal-pro-brain natriuretic peptide(NT-proBNP) and the cardiac function in patients with chronic heart failure. Method: Eighty-four patients of chronic heart failure caused by coronary heart disease were randomly divided into treatment and control group (n=42 each). The control group was applied with routine treatment and the treatment group was given the routine treatment and SBP. After four weeks,the level of plasma NT-proBNP and the cardiac function were detected. Result: Compared with those before treatment, left ventricular end distolic diameter(LVEDD), left ventricular ejection fraction(LVEF),cardiac output(CO) and left ventricular and systolic diameter(LVESED) were improved obviously in both groups. However,these indices in the treatment group were better than those in the control group(P<0.05,P<0.01).As plasma NT-proBNP was concerned, the reduce in the treatment group was more significantly than that of the control group(P<0.05).The clinical efficacy of the treatment group was more significantly than that of the control group(P<0.05). Conclusion: SBP can significantly enhance the cardiac systolic and diastolic function and decrease plasma level of NT-proBNP.关键词：Shexiang Baoxin pills;heart failure;cardiac function;n-terminal-pro-brain natriuretic peptide
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy on Huayu Xiaocuo decoction in treatment of nodular cystic type acne. Method: One hundred and eight cases of nodular cystic acne patients were randomly divided into treatment group and control group, 54 cases each group. The treatment group was given Huayu Xiaocuo decoction, and control group was given Vitamin A acid capsules, 10 mg each time, twice a day. Effects were observed in both groups for 8 weeks after administration. The skin lesions were recorded at begnning of treatment,and week 2,4,8,the signs and symptoms were scored to determine the clinical efficacy. During treatment adverse reactions and recurrence were observed. The total efficiency rate was given as cured and effective. Rusult: In treatment group the improvement of clinical symptoms and clinical symptom score was more significant than that in the control group. The total efficiency rate in treatment group was 88.89%, and in control group being 68.52%. Conclusion: Huayu Xiaocuo decoction can treat nodular cystic type acne effectively.关键词：Huayu Xiaozuo decoction;nodular cystic type;acne;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy and mechanism of action of Shenmai injection for cancer related fatigue (CRF), Method: According to the clinical application of Shenmai injection, one hundred and fifty-six patients with CRF were divided into 86 cases of the observation group and 70 cases of the control group. The control group was treated by only conventional symptomatic treatment, while the observation group was treated by Shenmai injection in addition to conventional symptomatic treatment. The treatment in two groups lasted for 4 weeks. Piper fatigue revised scale, the indicators of quality of life scale, the syndrome score in traditional Chinese medicine and the immune function were evaluated. Result: The observation group showed a decrease in the number of CRF patients compared with the control group (P<0.01). The physical function, emotional function, social function and total score ratings in observation group were better than those in the control group (P<0.01). In observation group, levels of IgG, IgA, and IgM were higher than those in the control group, levels of IL-1 and TNF-α is were lower than those in the control group (P<0.05, P<0.01). The score of deficiency of qi and yin in the observation group was lower than that in the control group (P<0.01). Conclusion: Shenmai injection can improve immune function and reduce the score of deficiency of qi and yin, therefore it alleviates CRF and improve the quality of life.关键词：Shenmai injection;cancer related fatigue;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of metformin treatment for metabolic syndrome induced by atypical antipsychotic drugs. Method: The patients suffered from metabolic syndrome induced by atypical antipsychotic were randomly divided into the treatment group (hydrochloric acid metformin 500 mg 1 time, daily) and the control group (diet control+exercise). Treatment lasted for 3 months. Before and after treatment the following parameters were observed: height, weight, waist circumference (WAIST), blood fat, fasting blood sugar (FPG), blood glucose 2 h after OGTT (PG 2 h), blood pressure (SBP/DBP), body mass index (BMI). Result: After the treatment, BMI, WAIST, FPG, PG 2 h, SBP/DBP, serum triglyceride (TG), high-density lipoprotein (HDL) all were improved significantly (P<0.01). In the control group, after the intervention, only BMI, WAIST, FPG and TG were obviously improved (P<0.05). Except HDL-C, all the other parameters in the treatment group were improved better than those in the control group·m-2vs (0.48±1.18)kg·m-2,(7.53±4.35)cm vs(2.21±5.37)cm,(0.85±0.82)mmol·L-1vs(0.22±0.57)mmol·L-1,(1.31±1.00)mmol·L-1vs(0.21±0.71)mmol·L-1,(0.92±0.55)mmol·L-1vs(0.32±0.65)mmol·L-1,(0.19±0.21)mmol·L-1vs(0.06±0.3)mmol·L-1,(4.59±6.59)mmHg vs(0.76±4.30)mmHg,(4.25±7.03)mmHg vs(0.31±5.63)mmHg,P<0.01]. Conclusion: The interventions in both groups could improve metabolic syndrome caused by atypical antipsychotic drugs, but metformin showed better effects compared with diet control+exercise.关键词：atypical antipsychotic drugs;metabolic syndrome;metformin;diet and exercise
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy of Zhizhu Kuanzhong capsule in the treatment of functional dyspepsia (FD). Method: Eighty cases reached the standard of FD were randomly divided into treatment group, control group (n=40 each). Patients in two groups were orally given domperidone (10 mg, 3 times daily), and asked to eat easy to digest food, to avoid other drugs with potential influences. The patients in treatment group were orally treated with Zhizhu Kuanzhong capsule (3 tablets, tid). Seven days were considered as a treatment course. After 3 courses of treatment, the outcome was compared for the two groups. Result: The cure rate was 75% in treatment group, and 62.50% in control group, with significant difference (P<0.05). The total efficiency was 95% in treatment group, and 85% in control group, with significant difference (P<0.05). The side effect scores of postoperative nausea and vomiting, loss of appetite, abdominal discomfort, belching, heartburn, acid reflux symptoms in treatmentt group were (0.94 ±0.27), (0.95±0.44), (0.75+0.37) points (0.25±0.45), (0.47±0.12), (0.76±0.22) accordingly, in the control group they were (1.76±0.32), (1.51±0.55), (1.75±0.52), (1.78±0.41), (1.45±0.54), (1.45±0.51) respectively. In two groups, the scores were decreased obviously after the treatment compared with those before the treatment (P<0.05). The treatment group was better than the control group (P<0.05). After treatment, normal slow wave before meals, normal slow wave after meals and the main power ratio before and after meals in the treatment group were (43.62±18.60)%, (52.32±1.54)%, (2.87±2.55), while in the control group (40.75±15.58)%, (46.32±16.64)%, (2.51±1.23) respectively. In two groups the treatments improved the parameters significantly (P<0.05). The treatment group was better than the control group (P<0.05). Conclusion: Clinical efficacy of Zhizhu Kuanzhong capsule for the treatment of FD is satisfactory.关键词：dyspepsia;functional;Zhizhu Kuanzhong capsule;domperidone
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of lithagogue decoction of Herba Desmodii on relieving pain and lithagogue after extracorporeal shock-wave lithotripsy (ESWL) surgery. Method: Eighty-six patients with ESWL surgery were randomly divided into two groups, the treatment group (43 cases) and the control group (43 cases). The treatment group was orally given lithagogue decoction of Herba Desmodii, while the control group was injected progesterone. The pain-relieving time and the lithagogue time were recorded respectively in the two groups. Result: The pain-relieving time in the treatment group(9±4.1)h was shorter significantly than that in the control group(11±6.4)h(P<0.05). The lithagogue time in the treatment group(7±3.1)d was shorter significantly than that in the control group(10±3.8)d (P<0.01). Conclusion: The lithagogue decoction of Herba Desmodii has both pain-relieving and lithagogue effects after ESWL surgery.关键词：lithagogue decoction of Herba Desmodii;progesterone;ureteral calculi;extracorporeal shock-wave lithotripsy
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical curative effect of Fangxiang Huashi decoction in treatment of diabetic gastroparesis with damp-pathogen syndrome. Method: Sixty-six cases with damp-pathogen syndrome in diabetic gastroparesis patients were randomly divided into treatment group and control group,33 cases in each. Treatment group was given the Fangxiang Huashi decoction and control group received mosapride citrate dispersible tablets by oral administration. The treatment course lasted 6 weeks. The main symptoms and the time of gastric emptying were observed before and after treatment. Result: The total effective rate in the treatment group was significantly better than the control group (P<0.05), the clinical symptoms in treatment group were better than those in control group (P<0.05). Conclusion: The Fangxiang Huashing decocotion has remarkable curative effect. In treatment of diabetic gastroparesis with damp-pathogen syndrome.关键词：diabetic gastroparesis;eliminating dampness with aromatics;clinical efficacy
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发布时间：2024-01-04 - 摘要：Objective: To explore application of Xuebijing in treatment of severe pneumonia and its effects on inflammatory factors and cellular immunity. Method: Fifty patients were chosen with severe pneumonia divided them into control group and treatment group receiving basic treatment including anti-infection, nutritional support, and mechanic ventilation and so on. Treatment group received an additional treatment of Xuebijing Injection during the treatment. Result: Compared with control group, the total effective rate in treatment group was increased significantly (84.00% vs 96.00%) (P＜0.05); serum level of interleukin-1(IL-1), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) decreased significantly after treatment (P＜0.05), a more significant decrease was found in treatment group than in control group (P＜0.05); compared with control group, CD4+/CD8+ ratio increased significantly after treatment (P＜0.05). Conclusion: Application of Xuebijing based on regular therapy shows a fine effect in treatment of severe pneumonia by decreasing inflammatory level and increasing cellular immunity.关键词：severe pneumonia;Xuebijing;inflammatory factor;cellular immunity
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发布时间：2024-01-04 - 摘要：Index the literatures in CNKI, ACS, ScienceDirect, and Wiley Online Library databases from 1993 to 2012 choosing titles or keywords as 'Polymethoxylated Flavones (PMFs)'. Up to now, PMFs reported home and abroad has almost 80, and they were found widely distributed in the plants, such as Rutaceae and Rubiaceae . However, there is little systemic summary on them. This paper sums up the structural features, distribution, preparation methods and pharmacological effects of PMFs, and provides useful references for the further research for the development of plant resources of PMFs.
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发布时间：2024-01-04 - 摘要：To analysis the affect on the saponins of the medicinal plants bring by the major environmental factors. We analysis the relationship between the environmental factors and the composition of the saponins of the medicinal plants. the environmental factors, such as origin, harvest time, light, temperature, and cultivation measures etc. analysis the mechanism to find the theoretical basis for these phenomenons. The environmental factors such as origin, harvest season, growth years, light and temperature in the prosess of growth, and the regulate of the fertilizer or water in the prosess of cultivation, all of these have significant impact on the saponin of the medicinal plant. These results have certain theoretical support. The production of high quality of Chinese herbal medicines should consider the suitable area for the cultivation, and to determine the reasonable harvest time. The scientific cultivation practices in the cultivation is the effective means of to improve production and active ingredients of the medicinal plants. It is als a good way to take the appropriate short-term stress during the progress of optimize the cultivation conditions.关键词：environmental factors;medicinal plants;saponin;environmental stress
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发布时间：2024-01-04 - 摘要：To explore the relations between the P-glycoprotein and the nature of traditional Chinese medicine (TCM), which will be helpful to a better application of the P-glycoprotein in the study on the nature of TCM. Based on the generalization of the latest progresses of the studies on the relation of the P-glycoprotein and the Meridian-guiding herbs, compatibility relations and detoxification by compatibility, we came up with the thoughts of the nature analysis of TCM by P-glycoprotein. We also analyzed the technical characteristics of the study methods of the relationship between the nature of TCM and the P-glycoprotein, including cell model method, in situ perfusion experiment, gene knock-out animal method, and computer simulation. Since some ingredients in the TCM can affect the activity of the P-glycoprotein, by which the interaction of the TCM in vivo can be illustrated from the angle of the transmembrane protein. By the theoretical and methodical application of the P-glycoprotein, new idears for the study of the theoretical study of the nature of the TCM can be worked out, which is good for the mutual osmosis of subjects and the improvement of the application and the modern research of the traditional theory of TCM.关键词：P-glycoprotein;theory of the nature of TCM;absorption-distribution-metabolism-excretion;compatibility of Chinese traditional medicine
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发布时间：2024-01-04 - 摘要：To study HIV/AIDS research progress of traditional Chinese medicine(TCM) syndrome standardi-zation. The team researched in positive TCM syndrome standardization of HIV/AIDS, based on the study of philology, combined with clinical research using the method of evidence-based medicine in recent years. It's preliminary draws up the seven AIDS basic syndrome differentiation standard scale. The syndrome diagnostic scale formulation is has the important meaning to the HIV/AIDS theory of traditional Chinese medicine, Chinese medicine building syndrome diagnostic scientific, preciseness and reliability and clinical evaluation method of the development.关键词：AIDS;TCM syndromes;standardization;practice
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发布时间：2024-01-04 - 摘要：Acute lung injury (ALI) is a common clinical critical disease, due to direct or indirect injury factors. It presents as the damage of alveolar epithelial cells and capillary endothelial cells, resulting in diffuse pulmonary interstitial and alveolar edema, leading to acute hypoxemic respiratory insufficiency. There are few effective drugs for the treatment of ALI so far. Complex prescriptions of traditional Chinese medicine (TCM) have increasingly shown their obvious advantages and further mechanism for the treatment of ALI. In this review, we introduced some related advances on account of the mechanisms including anti-inflammation, regulating immunological function, anti-oxidation, modulating abnormal coagulation and fibrinolysis, up-regulating of gaquaporins (AQPs) and attenuating pulmonary fibrosis since recent three years. What's more, the association between therapeutic principle of TCM and modern pharmacological mechanisms is summarized, in order to provide some references and clues to the drug development for ALI.关键词：acute lung injury;traditional Chinese medical prescription;mechanism;therapeutic principle of TCM
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