最新刊期
卷 19 ， 期 7 ， 2013
- 摘要：Objective: To investigate in vivo release behaviour of 5-aminosalicylic acid(5-ASA) colon-specific pellets in rats, which based on osmotic pump enzyme trigger principle, and evaluate its colon localization release characteristics. Method: 5-ASA colon-specific pellets (the pellet group) with 5-ASA sodium carboxymethyl cellulose suspension(the control group) were given to rats by intragastric administration respectively, then got blood from rat heart in predetermined time intervals, and removed the stomach, small intestine, cecum, colon and their contents, relative targeted release index(DDI value) of them were calculated;Pellets' surface structure of different parts were observed by scanning electron microscope. Result: DDI value of the pellets group in the stomach, small intestine, cecum and colon were 11.09, 12.08, 187.25, 181.68, respectively.We could observe that pellets had clearly visible aperture in the colon by scanning electron microscope. Conclusion: 5-ASA colon-specific pellets had good drug release in colon-specific.关键词：5-aminosalicylic acid;pellets;colon-specific drug delivery system;osmotic pump enzyme trigger;in vivo drug release
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction and purification procedures of antioxidant activity polysaccharides from Brassica rapa. Method: With the crude polysaccharides yield, the content and antioxidant activity of total polysaccharides as indicators, orthogonal design was used to optimize extraction technology by taking extraction times, solid-liquid ratio and extraction time as factors;With the removal rate of pigment and the loss rate of polysaccharides as comprehensive evaluation index, effects of bleaching temperature, adsorption time and activated carbon dosage on decoloration technology were investigated by orthogonal test. Result: Optimum extraction technology was as following:solid-liquid ratio 1:30, extracted 3 times at 90 ℃ with 2 h each time;The best decoloration technology was:absorbed 40 min with 3% activated carbon at 60 ℃.Under these conditions, purity of polysaccharides from B. rapa was up to 4.66%, the IC50 value of DPPH free radical scavenging was 6.71 g·L-1. Conclusion: These optimized extraction and purification processes were stable and reliable, a high level of polysaccharides with fewer impurities was obtained, which indicated that optimized processes were suitable for industrial production of polysaccharides from B. rapa.Meanwhile, these results indicated that polysaccharides from B. rapa had strong antioxidant activity, and worthy of further study.关键词：Tibetan medicine;Brassica rapa;polysaccharide;extraction technology;antioxidant
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology parameters of total flavonoids from flowers of Camellia semiserrata with HP-20 macroporous resin. Method: With elution ratio and purity of total flavonoids as indexes, technology parameters of HP-20 macroporous resin were investigated by single factor test, including static saturated adsorption amount, dynamic saturated adsorption amount, the concentration and amount of alcohol, et al. Result: Static saturated adsorption amount was 50.5 mg per gram resin.Optimum purification technology was as following:the volume of sample liquid 9 BV, washed impurity with 3 BV of deionized water and 3 BV of 15% ethanol, eluted with 10.5 BV of 35% ethanol at flow rate of 2 BV·h-1.Under these process conditions, purity of total flavonoids was up to 54.24%, elution rate of total flavonoids was 29.83%. Conclusion: HP-20 macroporous resin had good purification effects on total flavonoids from flowers of C. semiserrata, this optimized purification process was reasonable, stable and feasible.关键词：flowers of Camellia semiserrata;HP-20 macroporous resin;total flavonoids;technology conditions
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of total tannins from leaves of Apocynumm venetum. Method: With the content of total tannins as index, single factor test was used to investigate extraction methods, extraction solvent, solid-liquid ratio and other factors on extraction technology;With extract amount and yield of total tannins as comprehensive evaluation index, orthogonal test was used to optimize extraction technology of total tannins from leaves of A. venetum with liquid-solid ratio, extraction time and times as factors. Result: Optimum extraction process was A2B3C3, that was as following:reflux extracted 3 times with 20 times the amount of methanol, 3 h each time. Conclusion: This optimized extraction technology was stable, reasonable and feasible, it could be as an industrial production technology for leaves of A. venetum.关键词：leaves of Apocynumm venetum;total tannins;extraction technology;orthogonal test
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining the content of eugenol and imperatorin in compound Nanxing pain paste formed with different stroma, and compare the loss rate of these two components during preparation process. Method: HPLC was adopted performing on a C18 column(4.6 mm×250 mm, 5 μm) with a mobile phase of methanol(A)-1% acetic acid (B), the gradient elution program was as follows:0-30 min, 46%A;30-32 min, 46%-61%A;32-34 min, 61%A;34-42 min, 61%-47%A;42-60 min, 47%A;The detection wavelength was set at 280 and 300 nm, the column temperature was at 30 ℃.Quality of these prepared three kinds of paste were comprehensively compared with the loss rate of index components during preparation process as indexes. Result: Eugenol was linear in the range of 0.018 31-3.662 7 μg(r=0.999 9), imperatorin was linear in the range of 0.013 22-0.528 6 μg(r=0.999 9).The loss rate of eugenol in compound Nanxing pain paste formed with different stroma were all large, however the loss rates of imperatorin were all small.Then the loss rates of eugenol in gel ointment of compound Nanxing pain paste were all smaller than solvent and emulsion transdermal patch of compound Nanxing pain paste. Conclusion: This established determination method was accurate and had satisfactory efficacy, it could determine the content of eugenol and imperatorin in these three kinds of compound Nanxing pain paste, and compound Nanxing pain gel ointment was more superior to be prepared as patch.关键词：compound Nanxing pain paste;gel ointment;solvent transdermal patch;emulsion transdermal path;eugenol;Imperatorin;HPLC
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize preparation technology of total saponins from Panax notoginseng transdermal gel ointments and establish its content determination method. Method: With adhesive power, appearance, coating properties, et al as comprehensive indexes, molding technology of total saponins from P. notoginseng gel ointments was optimized by orthogonal test.The content of total saponins from P. notoginseng was determined by HPLC. Result: The best formulation was as following:NP-700 and CMC (4:1) 5 g, kaolin 3.5 g, aluminium glycinate 0.18 g, glycerin and propylene glycol (2:1) 25 g, PVP-K90 0.5 g, PVPP 0.5 g, PVA 0.25 g.The linear range of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.42-3.36 μg(r=0.999 6), 4.82-38.56 μg(r=0.999 4), 1.24-9.92 μg(r=0.999 7). Conclusion: This optimized preparation technology of gel ointments was stable and practical.Established HPLC method was simple, reliable, and suitable for content determination and quality control of the preparation.关键词：total saponins from Panax notoginseng;gel ointments;orthogonal test;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology conditions of tannin from Diascorea cirrhosa. Method: With the content and retention rate of tannin as indexes, different macroporous resin properties of absorption and desorption were observed by static absorption method, purification technology conditions of tannin from D. cirrhosa was optimized by single factor test and L9(34) orthgonal design. Result: AB-8 type macroporous resin was chosen, its optimum purification technology conditions were as followings:sample amount no more than 17.25 mg per gram of resin, washed impurity with 3 BV water, and eluted by 1.5 BV 40% ethanol with flow rate of 0.5 BV·h-1, collected the eluent.The content and retention rate of tannin were 82.02%, 64.39%, respectively. Conclusion: This optimized technology was stable, feasible and reasonable, it could provide experimental basis for industrial purification of tannin in D. cirrhosa.关键词：Diascorea cirrhosa;AB-8 type macroporous resini;purification process;Orthogonal Design;tannin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Magnolia officinalis by central composite design/response surface methodology. Method: Taking ethanol concentration, extraction time and the amount of solvent as independent variables, overall desirability of dry extract yield and the content of magnolol and honokiol as dependent variable, each level of independent variables was fitted by multiple linear regression and binomial formula fitting, extraction process was optimized by response surface method, and prediction was carried out through comparing the observed and predicted value. Result: Optimum extraction technology was as following:extracted 3 times with 10-fold the amount of 60% ethanol for 95 minutes each time. Conclusion: This optimized extraction technology of M. officinalis was reasonable, stable and feasible with high accuracy, it could be extended to large-scale production applications.关键词：Magnolia officinalis;central composite design;response surface methodology;magnolol;honokiol;extract
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total flavonoids in Geranium wilfordii. by central composite design/response surface methodology. Method: Independent variables were ethanol concentration, reflux time, the amount of solvent and reflux temperature, dependent variable was extraction rate of total flavonoids, linear or no-liner mathematic models were used to estimate the relationship between independent variables and dependent variable, response surface methodology was used to optimize extraction technology, and prediction was carried out through comparing the observed and predicted value. Result: Regression coefficients of binomial fitting complex model was as high as 0.960 9, optimal extraction technology conditions were as followings:extracted 2 times with 35 times the amount of 65% ethanol at 90 ℃, 70 min per time.Bias between the observed and predicted value was-1.25%. Conclusion: Central composite design/response surface methodology was convenient and highly predictive for optimizing extraction technology of total flavonoids.关键词：Erodium stephanianum;central composite design;response surface methodology;total flavonoids;extraction technology
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To determine the contents of six kinds of alkaloids from Aconitum kusnezoffii and its processed products, evaluate its safety and effectiveness of clinical application. Method: The contents of six kinds of alkaloids was determined simultaneously by HPLC, and compare content changes of six kinds of alkaloid before and after processing. Result: The content of monoester alkaloids from A. kusnezoffii was 0.408 1-1.718 2 mg·g-1, and the content of diesterifiable alkaloids was 0.660 4-8.653 2 mg·g-1;The content of diesterifiable alkaloids greatly reduced and the content of monoester alkaloids increased significantly after processing. Conclusion: The content of six kinds of alkaloid had significantly difference from A. kusnezoffii in different areas, processing technologies of steam and cooking had some scientific, the content of diesterifiable alkaloids was less than 0.04% and monoester alkaloids often beyond limited demand after processing.关键词：Aconitum kusnezoffii;diesterifiable alkaloids;monoester alkaloids;processing technology;content determination
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Guyu liniment. Method: Orthogonal test was employed to optimize extraction procedure, including the concentration of ethanol, the amount of solvent, extraction time and extracting times.The content of hydroxy safflor yellow A(HSYA) was used as index which was determined by HPLC. Result: Optimum extracting process for Guyu liniment was as follows:reflux extracted 2 times with 12 times the amount of 70% ethanol, 1.5 h per time. Conclusion: This optimized process was stable and feasible with relatively high content of HSYA.关键词：Guyu liniment;extraction process;orthogonal test;hydroxy safflor yellow A;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize supercritical carbon dioxide fluid extraction (SFE-CO2) process of volatile oil from the roots of Clematis chinensis, and components of volatile oil were analyzed by GC-MS. Method: With yield of volatile oil as index, effects of extraction time, particle size of raw materials, fluid extraction pressure and temperature on SFE-CO2 process of volatile oil from the roots of C. chinensis were investigated by single factor test and orthogonal test, and chemical constituents of volatile oil were analyzed by GC-MS. Result: Effects of three factors on yield of volatile oil were in order of extraction pressure>extraction time>extraction temperature;Optimal fluid extraction technology was as following:extraction pressure 20 MPa, extraction time 1.5 h, temperature 45 ℃;Yield of volatile oil under these conditions was 0.44%.156 compounds were identified by GC-MS and they were accounting for 85.03% of the total content of volatile oil, compositions that the relative content was more than 1% accounted for 51.78% of the total content of volatile oil. Conclusion: SFE-CO2 method had higher extraction efficiency than steam distillation, this optimized technology was stable and reliable.In addition, chemical compositions of volatile oil were complicated, and protoanemonin, the major bioactive component in volatile oil from the roots of C. chinensis, was found by GC-MS for the first time.关键词：Clematis chinensis;volatile oil;supercritical carbon dioxide fluid extraction process;orthogonal design;GC-MS
- 2
- |
- 4
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total flavonoids from Xinjiang Berberis heteropoda fruit. Method: The content of total flavonoids was determined by UV, which was as dependent variable, based on single factor test, with solid-liquid ratio, extraction time and ethanol concentration as independent variables, three factors and three levels Box-Behnken design was adopted, extraction technology conditions of total flavonoids from Xinjiang B. heteropoda fruit were optimized by response surface methodology. Result: Optimal extraction conditions were as followings:extracted 2 h with 33 times the amount of 43% ethanol at 80 ℃, yield of total flavonoids was 24.75 mg·g-1, which accounted for 97.6% of the predicted value(25.37 mg·g-1). Conclusion: Response surface methodology to optimize extraction technology of total flavonoids from Xinjiang B. heteropoda fruit was feasible and reasonable.关键词：Berberis heteropoda fruit;total flavonoids;response surface methodology
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize matrix composition and ratio of artemisia flavonoids cream. Method: L9(34) orthogonal test was employed to investigate effects of four factors including the amount of cetyl alcohol, octadecanol, emulsifiers, 1, 3-propylene glycol on matrix formulation, with appearance, stability, ductility, viscosity, pH value and microscopic observation as comprehensive evaluation index. Result: The best proportion of matrix prescription was as following:artemisia flavonoids 0.5%, cetyl alcohol 6%, octadecano 6%, emulsifier 8%, 1, 3-propylene glycol 4%, urea 2%, pure water 64%. Conclusion: This prepared cream was fine and uniform with suitable consistence and ductility, stable properties.The color of it was bright yellow, odor was special smell of traditional Chinese medicine, it was in line with the requirements.关键词：artemisia flavonoids;acne;cream matrix;orthogonal test;process ratio
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of ginger antihalation gel ointments. Method: With initial adhesive force, cohesive forces and appearance score as comprehensive evaluation index, orthogonal test was used to optimize formulation technology of ginger antihalation gel ointments with the amount of PAC-Na, PVP-k90, glycerin, aluminium glycinate as factors. Result: Optimum formulation was as following: PAC-Na-PVP-k90-glycerin-aluminium glycinate 8: 3: 45: 1. Conclusion: These prepared gel ointments had a smooth appearance, moderate viscosity, good moisture retention and good compatibility with antihalo active ingredients in ginger.关键词：orthogonal test;gel ointments;preparation process;ginger;motion sickness
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize microwave processing technology of Curcuma phaeocaulis processed with vinegar. Method: With the content of volatile oil, germacrone and curcumin as comprehensive indexes, effects of the amount of vinegar, moistening time, microwave intensity and processing time on microwave processing technology of C. phaeocaulis were investigated by orthogonal design. Result: The best processing technology was as following: the amount of vinegar 15%, moistening time 45 min, microwave heating with 60%, processing time 3 min. Conclusion: This optimized technology was simple, feasible and easy to control, it could be used as a new processing method of C. phaeocaulis processed with vinegar.关键词：Curcuma phaeocaulis;germacrone;curcumin;microwave processing;orthogonal design
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize enzymatic hydrolysis technology of genistin. Method: Materials of genistin with different size were prepared by ultrafine grinding technology, then genistin was hydrolysised by celluse to get genistein.With the conversion rate of genistein as index, single factor tests were used to investigate effects of enzymatic hydrolysis time, temperature, size and pH on enzymatic hydrosis technology, and then, preparation technology of genistein was optimize by central composite design response surface methodology, which was enzymatic from genistin ultrafine powder. Result: Optimum technology conditions to get genistein through enzymatic hydrolysis genistin ultrafine powder were as followings:pH 5.5 acetic acid and sodium acetate anhydrous buffer as reaction medium, temperature 49 ℃, reaction time 11 h and ratio of substrate-enzyme1: 1.Under these conditions, the conversion rate of genistein was (96.47±1.12)%, which comparing with genistin ordinary powder, the conversion rate increased 1.84 folds. Conclusion: This method of enzymatic hydrolysis genistin ultrafine powder by celluse to obtain genistein was simple, efficiency and suitable for modern large-scale production.关键词：genistin;ultrafine gringding;genistein;celluse;central composite design response surface methodology
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To optimize supercritical CO2 fluid extraction(CO2-SFE) conditions of four medicinal herbs in Shufushuang sprays including Cnidium monnieri, et al. Method: With the content of ostho1e and total extraction yield as comprehensive evaluation index, the content of osthole was determined by HPLC, CO2-SFE of four medicinal herbs in Shufushuang sprays was optimized by orthogonal design with extraction pressure, extraction temperature and the separating kettleⅠ temperature as factors. Result: Extraction pressure and temperature had significant effects on CO2-SFE, whereas the separating kettleⅠ temperature had no significant effects.Optimum CO2-SFE technology was A2B1C1, extraction pressure 30 MPa, extraction temperature 50 ℃, the separating kettleⅠ temperature 50 ℃. Conclusion: Optimized extraction technology of volatile oil was stable and feasible, it could be used in industrial production.关键词：Shufushuang sprays;CO2-supercritical fluid extraction technology;orthogonal design
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of different control samples on content determination of total saponins from Panax notoginseng. Method: With ginsenosides Rg1, ginsenoside Rb1, notoginsenoside R1 and P. notoginseng saponins as reference substance, taking HClO4-vanilin staining method, the content of total saponins in P. notoginseng was determined by ultraviolet spectrophotometry. Result: There were significant differences in determination of the content of total content from P. notoginseng among groups of different references. Conclusion: With UV spectrophotometry as content determination method for total saponins from P. notoginseng, we could choose P. notoginseng saponins as a reference substance, which could provide more reliable experimental data for determining the content of P. notoginseng and its preparations.关键词：Panax notoginseng;ginsenosides Rg1;ginsenoside Rb1;notoginsenoside R1;panax notoginseng saponins;UV Spectrophotometry;content determination
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprint of Ginger, dried Ginger and roasted Ginger to provide evidence for its quality control. Method: A gradient mobile system was applied to the measurement of ten batches of Ginger, dried Ginger and roasted Ginge. Mobile Phase was acetonitrile-water. The UV detection wave length was at 240 nm, and the flow rate was 0.8 mL·min-1. The chromatogram fingerprints were evaluated by the similarity evaluation software published by the committee of codex and calculate the similarity of ten batches of Ginger, dried Ginger and roasted Ginge. Result: The HPLC fingerprint of Ginger was established and six co-possessing peaks were selected separately as the fingerprint peaks of Ginger, dried Ginger and roasted Ginger. The similarity of ten batches of Ginger, dried Ginger and roasted Ginge was respectively more than 0.902, 0.911 and 0.929. Conclusion: The instrument precision, stability test and replicate test were in accord with the technical requirements of fingerprint. This method shows the high precision and reliability, and can be used to the quality control of Ginger.关键词：ginger;dried ginger;roasted ginger;HPLC;fingerprint
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the fingerprint method of Herba Bidentis and identify five species of Herb Bidens. Method: The methanol extraction of Herba Bidentis was separated on a Kromasil C18 column(4.6 mm×250 mm, 5 μm) eluted with Methanol-(0.1%phosphoric) acid water in linear gradient condition with DAD detected at 290 nm.Flow rate was 1 mL·min-1 and 10 μL was injected and the column temperature was 30 ℃. Result: The fingerprint of Herba Bidentis was established for 10 samples of different sources. Principal components analysis and clustering analysis were used in the identification of the samples, and the similitude was computed by computer. Conclusion: It was also the simple extraction and the most chemical components could be separated very well by HPLC. This method was stable, repeatable and feasible. And it provided scientific reference and effective method for the quality control of Herba Bidentis. The fingerprint could be used to distinguish Herba Bidentis.关键词：Herba Bidentis;HPLC;fingerprint;clustering analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: A gas chromatographic method was established for the determination of camphor, menthol, methyl salicylate in Guanjie Zhitong Plaster. Method: Three components in samples were extracted by steam distillation-extraction method. And camphor, menthol, methyl salicylate were separated by HP-INNOWAX column with FID detector, and the column temperature was 140 ℃. Result: The linear range of camphor, menthol, methyl salicylate is 0.402 7-4.026 9, 0.028 1-0.280 5, 0.121 0-1.209 9 g·L-1;and average recovery is 98.5%, 95.4%, 95.6% separately(n=6). Conclusion: The method is accurate and fast, and is reliable for the quality control of Guanjie Zhitong Plaster.关键词：Guanjie Zhitong Plaster;camphor;Menthol;methyl salicylate;gas chromatography
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To determine the five alkaloids of different parts in S. flavescens of different growth years. Method: The five alkaloids in S. flavescens were analyzed by HPLC. Result: Five alkaloids of different parts in S. flavescens of different growth years had their distribution law. The content of sophocarpine's distribution in different parts was leaf>stem>lateral root>taproot>reed head; the distribution of matrine was leaf>stem>reed head>lateral root>taproot; the distribution of oxysophocarpine was lateral root>taproot>reed head>stem>leaf; the distribution of sophoridine was lateral root>taproot>leaf>stem>reed head; the distribution of oxymatrine was lateral root>taproot>reed head>stem>leaf. With the increase of growth years, the five alkaloids increased; the fourth year was tend to be gentle. For example, the content of the taproot was: 13.58 mg·g-1 (first year), 20.49 mg·g-1 (second year), 27.74 mg·g-1(third year), 31.32 mg·g-1(forth year). Conclusion: This research can provide data for rational utilization and growth years determination of S. flavescens.关键词：Sophora flavescens;different growth years;different parts;HPLC;five alkaloids
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: A near-infrared quantitative method was developed for rapid determination of chlorogenic acid, luteoloside and isochlorogenic acid A in Chrysanthemum morifolium. Method: Near-infrared diffuse reflectance spectroscopy technology was adopted to collect NIR spectra of C. morifolium with HPLC analysis values as reference, the rapid determination method of chlorogenic acid, luteoloside and isochlorogenic acid A was established with partial least squares (PLS). Result: The correlation coefficients, the root-mean-square error of calibration (RMSEC) and the root-mean-square error of validation (RMSEP) of the calibration model for chlorogenic acid, luteoloside and isochlorogenic acid A in C. morifolium were 0.954 93, 0.015 7, 0.012 7, 0.984 50, 0.013 1, 0.017 6 and 0.998 28, 0.009 17, 0.005 11, respectively. The correlation coefficient of the true value and prediction value from external validation of 3 ingredients was 0.940 4, 0.941 2 and 0.944 6. Conclusion: The method is accurate, fast and simple, which can be applied for the rapid determination of large quantities of number of samples.关键词：Chrysanthemum morifolium;near-infrared spectroscopy;chlorogenic acid;luteoloside;isochlorogenic acid A
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of Shudantong Granules. Method: The jasminoidin, rhein and tetrahydropalmatine in Shudantong Granules were identified by TLC, and the content of jasminoidin was detected by HPLC. The separation was carried out on an Agilent ZORBAX C18column (4.6 mm×250 mm, 5 μm). The mobile phase consisted of acetonitrile-water (15: 85). The detective wavelength was set at 238 nm. The column temperature was kept at 30 ℃. The flow rate was 1.0 mL·min-1 and the injection volume was 10 μL. Result: The jasminoidin, rhein and tetrahydropalmatine could be identified by TLC and the calibration curve of jasminoidin showed a good linearity over the range of 15.1-151.0 mg·L-1 (r=0.999 9). The average recovery was 100.45% with RSD 0.48%. Conclusion: This method to identify three main ingredients in Shudantong Granules by TLC is simple and has a great repeatability. The negative control sample has no interference. The method to determine the content of jasminoidin in Shudantong Granules is rapid, accurate and its results have a good repeatability and recovery. This method can be used as the quality control method for this preparation.关键词：Shudantong Granules;jasminoidin;rhein;tetrahydropalmatine;TLC;content determination
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical compositions and their relative content of the petroleum ether immersion of the leaves of Gymnema sylvestre. Method: The chemical compositions of petroleum ether immersion of leaves of Gymnema sylvestre were separated and identified by GC-MS. Result: Thirteen compositions were found and 11 compositions were identified in the petroleum ether immersion. Conclusion: The principal chemical compositions in petroleum ether immersion were n-hexadecanoic acid, 2, 6, 10, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-tetracosa hexaene and octadecane, accounting for 23.28%, 26.34% and 21.35% of all quantity respectively. The research could provide scientific basis for chemical composition researches of leaves of Gymnema sylvestre.关键词：leaves of Gymnema sylvestre;composition analysis;GC-MS
- 4
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare the forsythoside, forsythin and rutin of Green Fructus Forsythiae and Grown Fructus Forsythiae from Shanxi Anze, the main ingredient content can provide the basis for application of forsythia in clinical. Method: The samples were extracted by ultrasonic extraction with methanol, and separated at 25 ℃ on a HITACHI C18 (4.6 mm×250 mm, 5 μm) column, eluted with methanol-acetic acid water as mobile phase. Flow rate was set at 1 mL·min-1 and the detection wavelength was set at 280 nm. Sample size was 10 μL. Result: The forsythoside, forsythin and rutin of Green Fructus Forsythiae were 2.38%, 0.37%, 0.26%; the content of the three components of Grown Fructus Forsythiae was 0.74%, 0.20%, 0.09%. Conclusion: The main ingredient content of the Green Fructus Forsythiae is higher than the Grown Fructus Forsythiae by HPLC.关键词：HPLC;Green Fructus Forsythiae;Grown Fructus Forsythiae;forsythoside;Forsythin;rutin
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for determination of 10 indicative components (aloe-emodin, rhein, emodin, chrysophanol, physcion, jatrorrhizine, epiberberine, coptisine, palmatine, berberine) in Rhubarb and Coptis chinensis. Method: Phenomsil BDS C18 (4.6 mm×250 mm, 5 μm) was adopted; the mobile phase was methanol-0.1% phosphoric acid (65: 35). The detection wavelength was set at 254 nm; the other mobile phase was acetonitrile-water (37: 63) with 0.34 g potassium phosphate and 0.17 g SDS; the detection wavelength was set at 345. The column temperature was maintained at 25 ℃. Result: The complete separation was obtained for the five compounds of Rhubarb. The complete separation was obtained for the five compounds of Coptis chinensis. Ten regression equation showing linear relationships between peak area and content of each compound were obtained. The average recoveries (n=6) of the compounds listed above were 100.79%, 99.38%, 99.83%, 98.94%, 98.86%, 100.20%, 99.53%, 99.76%, 100.07%, 100.37% and RSDs were 1.3%, 1.8%, 1.6%, 0.5%, 1.6%, 1.2%, 1.6%, 1.8%, 2.0%, 1.6%, respectively. Conclusion: This method is dependable, simple and practical, and may be used to control quality of Rhubarb and Coptis chinensis.关键词：Rhubarb;Coptis chinensis;Dahuang Huanglian Xiexintang;HPLC;Assay
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a new method of quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four components in Polygoni Cuspidati Rhizoma et Radix. Method: A HPLC method was developed as QAMS to determine polygonin, resveratrol, archen and emodin monomethyl ether in Polygoni Cuspidati Rhizoma et Radix. The relative correction factor (RCF) of other three components was determined by HPLC using archen as the internal reference substance. Content of the four components in 11 batches of samples, collected from different areas, was determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS. Result: RSD of QAMS method and external standard method was less than 3%, which indicated that no siginificant differences between the quantitative results of QAMS method and external standard method were observed. Conclusion: It is feasible and suitable to evaluate the quality of Polygoni Cuspidati Rhizoma et Radix by QAMS.关键词：quantitative analysis multi-components by single marker (QAMS);relative correction factor (RCF);Polygoni Cuspidati Rhizoma et Radix;polygonin;resveratrol;archen;emodin monomethyl ether
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of chromium in gelatin empty capsules which were digested in furnace by AAS. Method: The solution of gelatin empty capsules which digested by microwave or dissolved directly with water was injected into the graphite furnace of AAS, and determined the content of chromium with different temperature-programmed. Result: The calibration curves of both methods for chromium standard solution were prepared covering the concentration ranges of 4 to 40 μg·L-1. The correlation coefficients were all 0.999 9.The average recoveries of furnace digestion were in range of 96.2% to 101.2% and the RSD were 1.8% to 3.3%. The average recoveries of microwave digestion were in range of 97.51% to 103.1% and the RSD was 3.4% to 5.3%. The sample solution which would be injected directly and digested in furnace was stable within 4 hours. Conclusion: The results which determined by graphite furnace digestion and microwave digestion were consistent. This new method is easy, accurate and suitable for screening the exceeded content of chromium in the large quantities of gelatin empty capsules.关键词：gelatin empty capsules;atomic absorption-graphite furnace;furnace digestion;chromium
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents and establish a HPLC fingerprint of flavonoids parts of Smilax glabra. Method: UPLC-UV-MS technology was used to identify the chemical constituents of flavonoids parts of S. glabra; Diamonsil C18 column (4.6 mm×250 mm, 5 μm), methanol and 1.5% acetic acid solvent system gradient elution were applied, with flow rate of 0.8 mL·min-1 and UV detector at 280 nm. The column temperature was maintained at 30 ℃ and 10 μL was injected every time. HPLC method was used to establish a fingerprint. Result: Sixteen compounds were identified, among them, 9 chemical constituents were identified by direct comparison with the reference substances; in the fingerprint, 24 common peaks were confirmed. The results of similarity analysis of 10 batches of flavonoids parts of Smilax Glabra from different sources were above 0.99. Conclusion: The constituents of flavonoids parts of S. Glabra were clear. HPLC fingerprint has good repeatability, precision, strong specificity, and can be used to control the quality of flavonoids parts of S. Glabra. This research laid a foundation for further studies on pharmacodynamic material basis of flavonoids parts of Smilax Glabra.关键词：Smilax glabra;flavonoids parts;fingerprint;UPLC-MS;constituent analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the volatile constituents from pulp of two Cucurbita moschata (miben, chaotianmiben). Method: The volatiles were analyzed by head-space solid micro-extraction, coupled with GC-MS and Kovats indices for the first time. A quantitative analysis in percent was performed by peak area normalization measurements. Result: Twenty-nine compounds were identified from miben and 23 from chaotianmiben C.moschata. 96.55 % and 94.04% of the total essential constituents respectively. The two C.moschata. had 23 common components. Conclusion: Pyranone(16.66%), β-ionone(7.44%) were the highest components of the total essential constituents of C.moschata (miben); pyranone(14.87%), hexadecanoic acid (13.20%) were the highest components of the total essential constituents of C.moschata(chaotianmiben).The volatile constituents were different between the two C.moschata.关键词：Cucurbita moschata;volatile constituents;SPME;GC-MS
- 3
- |
- 4
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the method of determining the main lactones (senkyunolide A, coniferyl ferulate and ligustilide) in Ligusticum chuanxiong simultaneously, and to compare the content of them in L.chuanxiong from new and traditional producing areas. Method: The optimum condition of extracting three lactones was confirmed by single factor experiments. The content of the three was determined by RP-HPLC. Result: The content of the three lactones had significant differences between new and traditional producing areas, of which coniferyl ferulate was the most different, then senkyunolide A and ligustilide. Clustering analysis showed that there were no geographical distribution laws of the three lactones. Conclusion: The method was simple for operation, sensitive with good reproducibility and can be used for quality control of L.chuanxiong. The comparison of the three lactones from new and traditional producing areas can provide theoretical basis for introduction cultivation and quality evaluation of L.chuanxiong.关键词：Ligusticum chuanxiong;senkyunolide A;Coniferyl ferulate;ligustilide;new and traditional producing areas
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To compare the polysaccharide content from different Eremurus anisopterus at puberty and rest period. Method: The polysaccharide was extracted from the Eremurus anisopterus using water extraction and alcohol precipitation. Result: The polysaccharide content in flowering E. anisopterus is 3.98%, rest period polysaccharide content was 5.10%. Conclusion: Different E. anisopterus flowering period than rest period polysaccharide content as high as 1.12%, rest period recovery is relatively appropriate.关键词：Eremurus anisopterus;polysaccharide;colorimetry;harvest time
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of quercitrin in Radix Wikstroemia indicae. Method: HPLC was performed on Kromasil C18 column (4.6 mm×250 mm, 5 μm) at room temperature; the mobile phase consisted of acetonitrile-0.5% phosphoric acid solution (25: 75) at a flow rate of 1.0 mL·min-1, and the detection wavelength was set at 254 nm. Result: The calibration curves of quercitrin were linear in the range of 0.112-1.008 μg (r=0.999 1). The average recovery of quercitrin was 101.38% (RSD 2.78%), and the properties of sample solution kept steady within 24 hours. Conclusion: This method is simple, rapid, accurate.关键词：Radix Wikstroemia indicae;quercitrin;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish the quality standards for Zhierling Granules. Method: Rhizoma Atractylodis Macrocephalae and Leatherleaf Mahonia were identified by TLC. The content of hesperidin in Pericarpium Citri Reticulatae was determined by HPLC. Result: The method of TLC was highly specific with good reproducibility, the negative control without interference. The linear range of hesperidin was 0.12-0.60 μg (r=1.0). The average recovery rate was 99.90% and RSD was 1.073% (n=6). Conclusion: The method is simple, accurate and suitable for the quality control of Zhierling Granules.关键词：Zhierling Granules;quality standard;TLC;HPLC;hesperidin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To establish a determination method for the extraction of total flavonoids in Morus alba. Method: The sanggenon C was used as the authentic standard. The content of total flavonids was determined by the HCl-Mg reaction colorimetry, measurement wavelength was set at 481 nm. Result: The sanggenon C calibration curve showed a good linear relationship in the ranger of 146.8-734.0 mg·L-1 (n=5, r=0.999 8) and the average recovery was 96.74%, RSD 2.60% (n=6). Conclusion: The method established was reliable and accurate. It can be used to determine the content of total flavonoid in M. alba.关键词：Morus alba;total flavonoids;UV Spectrophotometry;Sanggenon C
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the essential oil from the rhizomes of Bellamcanda chinensis. Method: The essential oil was extracted by supercritical carbon dioxide extraction assay (SFE-CO2), and was separated and identified by gas chromatography-mass spectrometer (GC-MS). The relative content of each component was calculated by area normalization method. Result: Thirty-two compounds were identified, accounted for 93.87%. Conclusion: The major components were tetradecanoic acid, ethyl ester (17.38%), tetradecanoic acid (14.63%), hexadecanoic acid, ethyl ester (11.80%), et al. The study can provide quality control evidence and further exploitation of B. chinensis.关键词：Bellamcanda chinensis;supercritical carbon dioxide extraction;essential oil;gas chromatography-mass spectrometer
- 2
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To Study the chemical constituents of the Solidago virgaurea. Method: The compounds were isolated and purified from the plant with chromatography techniques and the structures were identified on the basis of spectral analyses and physicochemical properties. Result: Twelve compounds were isolated from S. virgaurea and their structures were identified as 2'-methoxybenzyl-2-methoxy-6-hydroxybenzoate, 2'-methoxybenzyl-2, 6-dimetho-xybenzoate, β-amyrin acetate, α-spinasterol, stigmasta-7, 22-dien-3-one, hexadecanoic acid, 2-hydroxy-6-methoxybenzoic acid, pedalitin, quercecetin, kaemferol, 3β-acetoxyolean-12-en-11-one-acetate, N-benzoylphenylalaninyl-N-benzoylphenylalaninate and protocatechoic acid. Conclusion: Compounds 11 was isolated from Solidago for the first time, compounds 5 and 10 were isolated from Solidago genus for the first time, compounds 1, 2, 3, 6, 7 and 12 were isolated from the plant for the first time.关键词：Solidago virgaurea;chemical constituents;structural identification
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the fatty acids of fruits of Xylocarpus granatum. Method: The gas chromatography and mass spectrometry was used in the study. The fatty acids of fruits of X. granatum were extracted by refluxing with anhydrous methanol and were abstracted with petroleum ether and were esterified with sulfuric acid-methanol. Result: In fruits of X. granatum, were detected to have eleven kinds of fatty acids. There were five kinds of unsaturated acids and the total amount were 81.97%.Respectively, the relative content of 9, 12, 15-octadecatrienoic acid was the highest. Conclusion: This study may provide scientific reference for the pharmacological use of X. granatum.关键词：Xylocarpus granatum;fatty acid;gs chromatography-mass spectrameter
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from stems and leaves of Scutellaria baicalensis. Method: Eleven compounds were isolated from the dried stems and leaves of S. baicalensis. Their structures were identified on the basis of physic-chemical constants and spectral analysis. Result: Compounds were isolated and identified as chrysin (1), 5, 7, 4'-trihydroxy-6-methoxyflavone (2), wogonin (3), 5, 4'-dihydroxy-6, 7, 3', 5'-tetramethoxyflavone (4), apigenin (5), isoscutellarein (6) baicalein-7-O-β-D-glucopyranoside (7), baicalin (8), apigenin-7-O-β-D-glucopyranoside (9), chrysin-7-O-β-D-glucuronopyranoside (10) and oroxylin-7-O-β-D-glucopyranoside (11). Conclusion: Compound 2, 4 and 6 were firstly isolated from this plant. Compound 9 and 11 obtained from stems and leaves of S. baicalensis for the first time.关键词：Scutellaria baicalensis;stems and leaves;chemical constituents;flavonoids
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the absorption kinetics of active components which was rhynchophylline and isorhynchophylline of Tiangou Jiangya capsules in rat's intestinal. Method: Using an improved rat everted gut sac model, rhynchophylline concentration and isorhynchophylline concentration were determined by HPLC. The concentration of cumulative absorption amount was calculated. The linear regression equation was figured through the concentration of cumulative absorption amount and time. The parameters were calculated. Result: The regression equation of rhynchophylline was Y=0.000 9X-0.008 5(r=0.908 6), Ka=9.0×10-4μg·min-1·cm-2, t1/2=12.8 h; the regression equation of isorhynchophylline is Y=0.008 6X-0.178 2(r=0.909 0), Ka=8.6×10-3μg·min-1·cm-2, t1/2=1.34 h. Conclusion: The absorption of rhynchophylline and isorhyncho-phylline conforms to the first-order kinetics in rats' intestine.关键词：Tiangou Jiangya capsules;rhynchophylline;isorhynchophylline;absorption kinetics;HPLC
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the influence of Polygalae Radix compatibility with Magnoliae Officinalis Cortex to the chemical composition in rats gastrointestinal liquid and plasma. Method: Treating plasma with methanol protein precipitation, the residues were analyzed with HPLC system (Synergi Hydro-RP C18 column, 4.6 mm×250 mm, 5 μm), with the mobile phase consisted of acetonitrile-water (51: 49), flow-rate of 1 mL·min-1, detected-wavelength of 294 nm, and column temperature of 30 ℃. Result: Magnolol and honokiol were detected in both of the Magnoliae Officinails Cortex group and the campatibility group's rats gastric intestinal juice and plasma, and the content of honokiol kind material in campatibility group's rats of gastric juice was obviously higher than the other group at every time piont. The content in rat's intestinal juice was higher than the magnoliae officinails group's rats after ig 150-480 minutes. There are only honokiol which had been detected in the rat's plasma, and the content of it in compatibility group's plasma was much higher than the other group after ig 60-480 minutes, with the trending of delayed absorption. Conclusion: After the compatibility of Polygala and Magnoliae Officinails Cortex, magnolol and honokiol were increased significantly in Magnolia, which may be relevanted with Magnolia relieving Polygala inhibition of gastrointestinal motility. First adopted this method to study the mechanism of Magnolia relieving Polygala inhibition of gastrointestinal motility, which had some innovation significance.关键词：Polygalae Radix compatibility with Magnoliae Officinalis Cortex;HPLC;gastric intestinal juice and plasma;magnolol;honokiol
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the anti-hypertensive effects of extracts from leaves of Apocynum venetum L. (ELA) and its mechanisms. Method: Twelve Beagle dogs were used to establish hypertensive mode by two-kidney two-clip method; endothelial cells were cultured to determine effects of ELA on nitric oxide(NO) output and endothelial nitric oxide synthase(eNOS) activities; then Western blot method was involved in current study to detect effects of ELA on eNOS, phospho-endothelial NOS(p-eNOS), phosph-phosphponositide-3 kinase(p-PI3K) and phosph-protein kinase B(p-Akt) expression. Result: Contractive and diastolic pressure of Beagle dogs showed degression in dose-dependent manner after ELA was administrated (P<0.01). Moreover, NO yield and eNOS activities of EA hy 926 cells were increased significantly when these cells incubated with ELA (P<0.01). It was also showed that ELA enhanced expression of eNOS, p-eNOS, p-PI3K and p-Akt in endothelial cells. Conclusion: ELA shows significant anti-hypertensive properties. The mechanisms may be associated with activation of PI3K/Akt pathway in endothelium.关键词：extracts from leaves of Apocynum venetum (ELA);hypertension;NO;eNOS;PI3K/Akt
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate whether the compound prescription Tongluo Jiannao and its main ingredients like geniposide (Gp) and Panax notoginseng Saponins (PNS) can promote the synaptic plasticity of nerve cells. Method: This experiment was divided into control group and treatment groups.Cell counting kit-8(CCK-8) kit was used to detect the cell proliferation curve after adding the drugs into the medium at the time interval 6, 12, 18, 24, 48 h. Western blotting was used to assay the synapse-associated proteins, such as drebrin and postynaptic density protein-95(PSD-95).PC12 cells induced by nerve growth factor (NGF) were used to observe if the drugs improve the amount of synapses. Result: Tongluo Jiannao, Gp and PNS couldn't influence the cell proliferation curve, and Gp could improve the expression of drebrin and PSD-95 significantly (P<0.01), Gp also could increase the amount of synapses (P<0.01). Conclusion: Tongluo Jiannao and geniposide could promote synaptic plasticity of nerve cells.关键词：compound prescription Tongluo Jiannao;panax notoginseng saponins;geniposide;synaptic plasticity;drebrin;PSD-95
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Hejie Lishi formula on rat alcohol chronic pancreatitis (ACP) through inhibiting apoptosis of pancreatic acinar cells. Method: Thirty animals were divided into two groups. Model group of 24 animals was established by feeding Lieber-De-Carli Liquid diet after 14 weeks. Normal group of 6 animals was given general diet. The model animals were injected 1.5, 1.5, 2.0, 2.5, 3 mg·kg-1 lipopolysaccharide (LPS) into caudal vein for five times at 8 weeks, once per one week. The model group was divided into control and different concentrations (21.7, 10.85, 5.425 g·kg-1) of Hejie Lishi formula group at 10 weeks. Then Hejie Lishi formula was given 4 weeks and once per day by ig. After treatment for 4 weeks, all rats were executed. The pathological changes of pancreas, apoptosis and its associated proteins were detected in pancreatic tissue. Moreover, apoptosis and cell growth were observed in AR42J cells with serum contained drug treatment. Result: There were inflammatory cell infiltration, bleeding and pancreatic acinar cell shedding in control group, compared with normal. Compared to control, Hejie Lishi formula decreased inflammatory cell infiltration and pancreatic acinar cell shedding (P<0.05). The high concentration of Hejie Lishi formula reduced apoptosis (P<0.05) and inhibited the activation of caspase-3 and caspase-9, increased the level of Bcl-2 and decreased the expression of Bax. The ratio of Bcl-2 to Bax in the high concentration of Hejie Lishi formula is 1.5-fold higher than control group. Compared with 30% normal rats serum, 30% containing Hejie Lishi formula serum enhanced pancreatic acinar cell growth (P<0.05). Apoptosis was reduced 32.66% by containing Hejie Lishi formula serum. Conclusion: High concentration of Hejie Lishi formula may prevent inflammatory and injury of pancreatic cells in ACP of rat. The mechanism may be involved in reducing apoptosis of pancreatic acinar cells through down-regulating caspase-3, -9 activities and bax expression, and up-regulating Bcl-2 expression.关键词：Hejie Lishi formula;alcohol chronic pancreatitis;pancreatic acinar cells;apoptosis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Chuangxiong Dingtong grainules (CXDT) on promoting blood circulation to dissipate blood stasis. Method: Seventy-two Kuenming mice were randomly divided into sex groups: normal control group(A), model group(B), compound Danshen dripping pills group(C), high-dose CXDT group(D), middle-dose CXDT group(E), low-dose CXDT group(F). Mice in group C-F were ig medicine 0.135, 1.75, 0.88, 0.44 g·kg-1 for 3 days. 1:1 000 epinephrine hydrochloride was dropped to the auricle of mice to induce local microcirculation disturbance. BI-2000 medical image analysis system was used to observe the microcirculation of mice at 10, 20, 30 minutes after dropping epinephrine hydrochloride. Sixty SD rats were grouped and gavaged as Kuenming mice. The qi-stagnancy and blood stasis rat model were established by epinephrine hydrochloride subcutaneous injection and ice water immertion. The femoral arteriovenous mixed-blood was used to assay hemorheology. Result: CXDT could significantly accelerate the microvascular flow rate of auricle microcirculation, expand its microvascular vein caliber and micro-artery diameter(P<0.05), increase the amount of open capillaries(P<0.05), significantly reduce the whole blood viscosity and the high shear blood relative index of blood stasis rat mode(P<0.05). On the other hand, CXDT could reduce the trend of the whole blood relative index. Conclusion: Chuanxiong Dingtong grainules has the effect of promoting blood circulation.关键词：CXDT;microcirculation;hemorheology
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Epimedii Folium on ovariectomy-induced osteoporosis in rats. Method: The female SD rats were divided into sham operated group(Sham), ovariectomized group(Model), high concentration Epimedii Folium with ovariectomized group(H-EF) and low concentration Epimedii Folium with ovariectomized group(L-EF).The serum Ca2+ were examined after administration for 45 days and 90 days. And the serum P, superoxide dismutase(SOD), malonaldehyde(MDA), estradiol(E2), testosterone(T)and alkaline phosphatase (ALP) were examined after 90 days ofadministration. The tibial slice was also analysized. Result: Compared with the model group, the serum Ca2+ was decreased [(2.48±0.39)mmol·L-1(P<0.05),(2.86±0.34)mmol·L-1(P<0.01)] by epimedium after 45 days of administration, and the serum MDA (8.4±1.3)mmol·L-1(P<0.01)for 90 days. But the serum ALP(4.4±0.89)U·L-1(P<0.05), P(4.61±0.82)mmol·L-1(P<0.05), E2(25.66±9.38) pg·L-1(P<0.05), T(1.95±1.21) ng·L-1(P<0.05) and SOD (110±13)U·L-1(P<0.05) were all increased by high concentration Epimedii Folium. Conclusion: It may be one of the mechanisms on osteoporosis that Epimedii Folium enhance estrogen and decrease ROS.关键词：Epimedii Folium;osteoporosis;ovariectomy
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Buyang Huanwu decoction(BYHWD) with different doses of Astragalus membranaceus on the proliferation of neural stem cells in the subventricular zone(SVZ) of the lateral ventricle after focal cerebral ischemia in rats. Method: Focal cerebral ischemia was induced by occlusion of the right middle cerebral artery for 90 min using the intraluminal filament model. BYHWD with different doses of A. membranaceus (120, 60, 30, 15 g, respectively, orally) and bromodeoxyuridine(BrdU, 50 mg·kg-1, ip) were treated on day 1 after ischemia once a day for 14 days. The modified neurological severity score(mNSS) and the corner test were used to evaluate sensorimotor function on 1, 7, 14 days after ischemia. BrdU/Nestin positive cells were examined by double immunofluorescence on 14 days after ischemia. Result: Compared with the model group, BYHWD with A. membranaceus (120, 60 g) significantly ameliorated neurological dysfunction and reduced the number of right turn on 7, 14 days after ischemia(P<0.05 or P<0.01).The number of BrdU/Nestin positive cells in SVZ was significantly enhanced on 14 days after ischemia. Conclusion: BYHWD with higher dose of A. membranaceus improves the proliferation of neural stem cells in SVZ and neurological function recovery following focal cerebral ischemia, the effect of 120 g A. membranaceus is the best.关键词：Buyang Huanwu Decoction;astragalus membranaceus;focal cerebral ischemia;neural stem cells;proliferation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the antiobesity function of the orlistat on diet-induced obese rats. Method: SD rats were randomized on the basis of their body weight and divided into control group, model group and orlistat group. Control group was fed with normal diet, model group and orlistat group was fed with nutritional diet. Orlistat group was administered for 7 weeks through the route of intragastric administration of orlistat(60 mg·kg-1) along with nutritional diet. Control group and model group were administered with 0.5% CMC-Na. The body weight, Lee's index, body fat, body fat/body weight and liver weight were measured. Result: The body weight [(306.52±37.02)g→(266.11±21.55)g,P<0.05], Lee's index [(309.45±7.25)→(301.34±6.69),P<0.05], body fat [(9.84±2.95)g→(4.67±1.98)g,P<0.05], and liver weight [(9.95±2.66)g→(7.61±1.08)g,P<0.05] of orlistat group significantly reduced compared to model group. Conclusion: Orlistat could significantly decrease body weight, liver weight, body fat and Lee's index of obese rats. The antiobesity function of the orlistat is significant.关键词：orlistat;antiobesity;obese rats
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate influences of compatibility of emodin and aconitine on the biomembrane of in vitro cultured interstitial cells of Cajal(ICC) from murine conlon. Method: ICCs of kunming mice's conlon were obtained by enzyme-digestion and culture in vitro. ICCs were cultured with compatibility of emodin(1, 0.3, 0.08 mg·L-1) and aconitine(1 g·L-1) to study the changes of malondialdehyde(MDA) and acid phosphatase(ACP). Result: Aconitine could increase the content of MDA and the activity of ACP in the cells(P<0.01). The compatibility of emodin and aconitine could reduce the content of MDA and the activity of ACP in the cells(P<0.05). Conclusion: Emodin can lighten the cell trauma of lysosomal membrane witch caused by aconitine.The compatibility of emodin and aconitine can protect the cell biomembrane.关键词：emodin;aconitine;ICC;biomembrance
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effect of the compalibility of glycyrrhizic acid and aconitine on nerve cell. Method: Neuron cells were prepared from neonatal SD rats and cultured in vitro. The MTT assay was used to determine how mixture of the glycyrrhizic acid and aconitine in different proportions infect the survival of nerve cell. Then the activity of Na+, K+-ATPase and the contents of [Na+], [K+] in the cells were measured using colorimetric method. Result: The activity of Na+, K+-ATPase and the contents of [Na+] in the cells were increased, while the contents of [K+] in the cells and the survival rate of nerve cell were decreased, after administration by the 2 g·L-1 aconitine for 30 min. The mixture of glycyrrhizic acid and aconitine in different proportions could lighten those changes caused by aconitine. Conclusion: Glycyrrhizic acid probably can decrease the toxicity of aconitine and antagonise the disturbance of internal environment in nerve cells. Besides, the best compatibility proportion is 1:2.关键词：glycyrrhizic acid;aconitine;neural cells;compatibility
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effects of Shenfu injection on the Ca2+-Mg2+-ATP ase, Ca2+-ATP ase, Na+-K+-ATP and Na+, Mg2+, K+, Ca2+ in the myocardial cell injured by nembutal. Method: The myocardial cell is gotten by trypsin digestion and purified by different velocity of adherence. After injured by 0.8% nembutal, the myocardial cell is administrated with 3 different concentrations of Shenfu Injection. Then mensurate the vitality of the Ca2+-Mg2+-ATP ase, Na+-K+-ATP ase and Ca2+-ATP ase and Na+, Mg2+, K+, Ca2+ in the cell 1 h later. Result: The Shenfu injection can increase the strength of the myocardial cell beating injured by nembutal, and enhance the activity of Ca2+-ATP ase and the Ca2+-Mg2+-ATP ase, inhibit the the activity of Na+-K+-ATP, enzyme increase the content of Na+, Mg2+ and decrease the content of K+, Ca2+ in the myocardial cell. Conclusion: The Shenfu injection can protect the myocardial cell injured by nembutal, and the function is relevent to the effects of Shenfu injection on the Ca2+-Mg2+-ATP ase, Na+-K+-ATP ase, Ca2+-ATP ase and the content of Na+, Mg2+, K+, Ca2+ of myocardial cell.关键词：congestive heart failure;Shenfu Injection;Ca2+-Mg2+-ATP ase;Ca2+-ATP ase;Na+-K+-ATP ase;Myocardial cell
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity of (6R, 7S, 8S)-7α-[(β-glucopyranosyl)oxy] lyoniresinol from Tarphochlamys affinis. Method: With microtitration assay and ultra-violet-visible (UV-Vis) spectrophotometry, effect of (6R, 7S, 8S)-7α-[(β-glucopyranosyl)oxy] lyoniresinol on clearance rate of 1, 1-diphenyl-2-picrylhydrazyl radical 2, 2-diphenyl-1-(2, 4, 6 trinitrophenyl) hydrazyl(DPPH·), hydroxyl radical·OH and superoxide anion O-2· were determined with Vitamin C(Vc) as positive control. Result: (6R, 7S, 8S)-7α-[(β-glucopyranosyl)oxy] lyoniresinol had scavenging capability against DPPH·(half clearance rate concentration IC50 8.11 mg·L-1), ·OH(IC50 21.61 mg·L-1) and O-2·(IC50 10.30 mg·L-1) radicals and has a good dose-responses with the concentration. Conclusion: (6R, 7S, 8S)-7α-[(β-glucopyranosyl)oxy] lyoniresinol has the stronger antioxidant activity.关键词：Tarphochlamys affinis;free radicals;antioxidant activity
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effects of dl-3n-butyphthalide(NBP)on neuromotor function and its mechanism of microcirculation dysfunction following diffuse brain Injury(DBI) in rats. Method: One hundred and thirty-five male SD rats were randomly divided into sham group(21), model group(38), low-dose NBP treatment group(38) and high-dose NBP treatment group(38). Treatment groups were adiministrated after injury by intraperitoneal injection, once for 24 h, continued to 72 h rat diffuse brain injury model was established by using Marmarou's method. The laser Doppler flowmeter was used to assay brain tissue changes of blood flow after 24, 48, 72 h tannic acid-ferric chloride mordant staining was used to determine the marking microvessel density, brain tissue changes of morphous was measured by electron microscopy; behavioral tests were performed. Result: Compared with sham group, brain tissue was damaged, the cerebral blood flows decreased at 24, 48, 72 h. The neuroscores and the general movement ability were damaged in model groups. Compared with model group, the damage of brain tissue was decreased, the cerebral blood flows increased at 24, 48, 72 h(P<0.05).The vascular density increased(P<0.05).The neuroscores and the general movement ability enhanced in NBP groups. The above mentioned indexes changed more significantly in high dose of NBP group. Conclusion: NBP can improve neurological function after brain trauma injury, which is in part through attenuating the cerebral microcirculation damage.关键词：diffuse traumatic brain injury;cerebral blood flow;microcirculation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Lycium bararum polysaccharide (LBP) and LBP with interferon-inducible protein 10(CXCL10) on the differentiation of helper T cells in bearing mice. Method: H22 bearing mice model was established and randomized into six groups: the model group, high-and low dose group, LBP with CXCL10 group, CTX group, control group. Serum was separated from eye ball after two weeks of treatment. tumors, spleen, thymus were separated and calculated anti-tumor rate, thymus index and spleen index. Flow cytometry was used to detect the differentiation of Th1/Th2 in mice peripheral blood. Result: Compared with model group, tumor inhibitory rate in low-and high dose group, LBP with CXCL10 group was 37.83%, 12.50%, 14.14%; the rate of Th1/Th2 was 4.44±3.05, 2.48±2.93, 4.36±1.96, among them LBP low dose group and LBP with CXCL10 group was obvious(P<0.05). Conclusion: Low dose of LBP and LBP with CXCL10 can increase the rate of Th1/Th2 markedly in bearing mice.关键词：Lycium bararum polysaccharide;interferon-inducible protein 10;Th1/Th2;H22 hepatocarcinoma cell
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of three kind of steroid saponins, extract obtained from Cestrum nocturnum on the human hepatocellular carcinoma BEL-7404 cell proliferation and its mechanism in vitro. Method: Three kind of steroid saponins of the leaves of C. nocturnum(SSCN) were separated and identified their structures by solvent. The BEL-7404 cells were cultured in vitro and cell colony measurement technique assay was used to determine the inhibition of cell proliferation. Alteration of cell cycle and apoptosis rate was defected by flow cytometry(FCM). The single cell microgel electrophoresis technique was used to analyse effect of DNA damage of BEL-7404.The effects of SSCN on Topo II mediated-pBR322 DNA unwinding were measured by using agarose gel electrophoresis. Result: SSCN significantly inhibited the proliferation of BEL-7404 cells in a time and dose-dependent manner. The results from FCM assay indicated that the percentage of cells in S phase decreased, while the percentage of cells in G0/G1 and G2/M phases increased, the phenomenon of blockage appeared and cell death was induced. The bioactive components of SSCN have effect on DNA damaged and inhibited the Topo II mediated relaxation of supercoiled pBR322 DNA effectively. Conclusion: SSCN could effectively inhibit cell proliferation and induce BEL-7404 apoptosis. With a direct relation to the dose and time, which may be correlated with factors as follows:①SSCN can arrest the cell cycle at G0/G1 and G2/M phase; ②DNA damage and cleavage of SSCN on DNA double strands may be a potential mechanism. ③CNSN can inhibit the catalytic activity of Topo II.关键词：steroid saponins of Cestrum nocturnum;human hepatocellular carcinoma BEL-7404;apoptosis;topoisomerase Ⅱ
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of ethanolic extract of Mirabilis jalapa L. roots on glucolipid metabolization in diabetic rats. Method: Type 2 diabetes mellitus rats were induced by intraperitoneal injection 40 mg·kg-1 streptozotocin and a high-carbohydrate/high-fat diet. Rats were divided into following groups: normal group, model group, ethanolic extract of M. jalapa roots group (1, 2, 4 g·kg-1) and rosiglitazone group(4 mg·kg-1). After 8-weeks treatment, fasting blood glucose(FBG), hemoglobin(HbAlC), total cholesterol(TC), triglyceride(TG), serum insulin (FINS) levels and glycogen contents in liver and skeletal muscle were assayed. Result: Compared with the normal group, FBG, HbAlC, TC, TG, FINS levels were significantly increased, and insulin sensitivity index(ISI), glycogen contents in liver and skeletal muscle decreased significantly in the model group (P<0.01). Compared with the model group, the levels of FBG, HbAlC, TC, TG, FINS decreased significantly, and ISI, glycogen contents in liver and skeletal muscle increased significantly in the ethanolic extract of M. jalapa roots group(2, 4 g·kg-1)(P<0.01). Conclusion: ethanolic extract of M. jalapa roots possesses both potential hypoglycemic and hypolipidemic effects on diabetes.关键词：Mirabilis jalapa root;diabetes;hypoglycemic;insulin;hypolipidemic
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the anti-inflammatory and analgesic effects of dichloromethane, acetate, and n-butanol extracts from vinegar-processed Cynanchum komarovii and to ensure the active fractions. Method: The mice were divided into 11 groups respectively. The effects were investigated 3 days after drug administration.Hot plate and glacial acetic acid writhing test were adopted in Kunming mice to observe the analgesic effect and the experiment of mice ear swelling induced by xylene and paw edema induced by carrageenan were applied to study on the anti-inflammatory effect. Result: Compared with the model group, all the three extracts of dichloromethane, acetate, and n-butanol (400, 800, 1 600 mg·kg-1) from vinegar-processed C. komarovii could significantly prolong the time period of paw-licking and reduced the writhing times induced by glacial acetic acid in a dose-dependence (P<0.05, P<0.01). And then the different extracts could inhibit the ear swelling and paw edema, and decrease the content of prostaglandin E2(PGE2) in inflammation tissue. Conclusion: Dichloromethane extract was ensured to be the active fraction. After toxicity attenuation by vinegar-processing, the C. komarovii still has a stronger bioactivity that can prolong the time period of paw-licking and reduced the writhing times induced by glacial acetic acid and inhibit the ear swelling and paw edema, and decrease the content of PGE2 in inflammation tissue. And the anti-inflammatory effect showed a relationship with content of PGE2.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the effect of artesunate nano-liposomes on vascular endothelial growth factor (VEGF) and VEGF receptor expression in HepG2 cells, provide the basic theory for liver cancer treatment. Method: HepG2 cells were cultured in vitro. Expression levels of VEGF mRNA and VEGFR-2 mRNA in HepG2 cells were evaluated in the control group, the artesunate active pharmaceutical ingredient(API) group and the artesunate nano-liposomes group by using RT-PCR method, protein expression levels of VEGF and VEGFR2 in HepG2 cells were detected by Western blotting method. Result: Compared mean values between the three groups, the artesunate nano-liposomes and the artesunate API could decrease the protein expression levels of mRNA and of VEGF and VEGFR2 in HepG2 cells (P<0.05). The expression intensity of the artesunate nano-liposomes group was lower than that of the artesunate API group. Conclusion: Artesunate nano-liposomes could inhibit the formation of new blood vessels, suppress tumor cells and have the potential use for the treatment of liver cancer.关键词：artesunate;nano-liposome;liver cancer;VEGF;VEGFR2
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Jiangzhi Baoganfang on the metabolic disorders of glucose and lipid in type 2 diabetic rat with hyperlipemia. Method: Type 2 diabetic rat model was established by high-fat diet with tail vein injection of streptozotocin (STZ, 38 mg·kg-1).Model rats were randomly divided into model group, positive medicine group (metformin 0.18 g·kg-1), Jiangzhi Baoganfang group 9.54 g·kg-1 and normal control group. Then the rats were administered with corresponding drugs for 35 days.The fasting plasma glucose (FPG), glycosylated hemoglobin(HbA1c), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), malondialdehyde (MDA), superoxide dismutase (SOD), as well as TC, TG, MDA and SOD in liver were measured. Result: Compared with model group, the levels of FPG, HbA1c in serum, TC, TG, LDL and MDA in serum and liver were significantly increased while the level of HDL and the activity of SOD in serum and liver were significantly decreased in model group(P<0.05). The levels of FPG, HbA1c in serum, TC, TG, LDL and MDA in serum and liver were significantly reduced while the level of HDL and the activity of SOD in serum and liver was significantly higher in Jiangzhi Baoganfang group(P<0.05). Conclusion: Jiangzhi Baoganfang can adjust the metabolic disorders of glucose and lipid in type 2 diabetic rat with hyperlipemia, decrease the lipid levels in liver and improve the antioxidant capacity.关键词：Jiangzhi Baoganfang;diabetic hyperlipemia;metabolic disorders of glucose and lipid;MDA;SOD
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 -
Effect of Baogan Tablets on Hepatic Fibrosis Induced by Carbon Tetrachloride Complex Factors in Rats
摘要：Objective: To investigate the effect of Baogan tablets against CCl4 complex factors induced hepatic fibrosis in rats. Method: The SD rat were divided into six groups randomly: control group (NC), hepatic fibrosis model group, 3 different does drug group (27.55, 13.78, 6.89 g·kg-1) of Baogan tablets and a positive control group (colchicine tablets 0.2 mg·kg-1).The rats of NC group were given normal saline, the other group were induced by CCl4 complex factors. At the same time, the other groups were treated by Baogan tablets and colchicine tablets. The effects of drugs were observed by calculating the liver index, detecting liver function alanine aminotransferase(ALT), aspartate aminotransferase(AST), albumin(ALB) and observing the extent of liver fibrosis. Result: Compared with control group, the content of AST, ALT in serum, liver index, and the extent of liver fibrosis were increased significantly, ALB was decreased in the model group(P<0.01). Compared with the model group, the content of ALB in serum was increased significantly (P<0.01), and the extent of liver fibrosis was lessened significantly in the drug group (27.55 g·kg-1, P<0.05). Conclusion: Baogan tablets could improve the degree of liver fibrosis in rats.关键词：Baogan tablets;hepatic fibrosis;CCl4 complex factors- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the expression levels of hippocampal vascular endothelial growth factor(VECF), fms-like tyrosine kinase-1(flt-1), brain-derived neurotrophic factor(BDNF), and basic fibroblast growth factor(bFGF) in vascular dementia rats, thus studying the angiogenesis mechanism of moxibustion in vascular dementia(VD). Method: Ninty-eight rats were selected. The VD model was established by permanent bilateral occlusion of both common carotid arteries(2-VO).Then the modeled rats were rando mLy divided into 5 groups, ie, the memory deficit model group, the donepezil group, and the positive drug control groups·kg-1).The donepezil hydrochloride suspension was given to rats in donepezil group by gastrogavage(0.62 mg·kg-1).High(64 g·kg-1), middle(32 g·kg-1), and low(16 g·kg-1) dose of Miao medicine Michen decoction were respectively given to rats in the other three groups for 2 theapeutic courses, 4 weeks as one course. The learning and memory results were observed by the neuroethological score in morriswater maze experiment before treatment and by the end of the 2 course respectively. The expression levels of hippocampal VECF, flt-1, BDNF, and bFGF of all rats were detected using enzyme linked immunosorbent assay. Result: After 2 courses of treatment, statistical difference existed in the latent period, the error times, and the neuroethological score in the high, middle, and low dose of Miao medicine Michen decoction groups and the western medicine group when compared the model group (P<0.05 or P<0.01). statistical difference existed in the latent period and the neuroethological score between the middle dose of Miao medicine Michen decoction group and the western medicine group(P<0.01).Which indicated that the Miao medicine Michen decoction and western medicine showed significant effects in improving the latent period, decreasing the error times and the neuroethological score.Better results were obtained in the middle dose of Miao medicine Michen decoction group than in the western medicine group(P<0.01). Statistical difference of the average level of Hippocampal VECF, flt-1, BDNF, and bFGF in the High, middle, and low dose of Miao medicine Michen decoction groups and the western medicine group when compared the model group. Statistical difference of Hippocampal VECF, flt-1, BDNF, and bFGF in the middle dose of Miao medicine Michen decoction group and the western medicine group(P<0.01). Conclusion: Miao medicine Michen decoction showed confirmative effects in improving the behavioral score and memory performance in VD rats. Its mechanisms might lie in that Miao medicine Michen decoction regulated and controlled the expression levels of Hippocampal VECF, flt-1, BDNF, and bFGF in VD rats, and ultimately stimulated the repairing mechanisms of cerebral nerve injury.关键词：Miao medicine Michen decoction;vascular dementia;vascular endothelial growth factor;fms-like tyrosine kinase-1;brain-derived neurotrophic factor;basic fibroblast growth factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Yixinnao dripping pills on platelet aggregation and thrombus formation in rats, as well as the effects on coagulability activity. Method: The Wistar rats were divided into normal control group, aspirin group, Yixinnao dripping pills high, middle and low dose group(906, 453, 226 mg·kg-1, ig, accordingly). The effects of Yixinnao dripping pills on platelet aggregation in vivo were measured by the model induced by ADP, thrombin and CaCl2.Coagulation time in acute blood stasis rat models was determined by the glass capillary method and the effects were also evaluated on prothrombin time (PT) and thrombin time (TT) in these groups. The inhibitory action of Yixinnao dripping pills on the thrombus formation was investigated by the model of artery-vein bypass thrombosis. Result: Yixinnao dripping pills in the high, middle and low dose could signifiinhibit platelet aggregation induced by ADP, thrombin and CaCl2, respectly (P<0.05 or P<0.01). It could also lengthen coagulation time, TT and PT in different degree (P<0.05 or P<0.01).Moreover, Yixinnao dripping pills (906, 453 mg·kg-1) could markedly restrain thrombus formation, reduce thrombus weight (P<0.05 or P<0.01). Conclusion: Yixinnao dripping pills showed the function of anti-thrombosis, and exerted the effects on inhibition of platelet aggregation and coagulability.关键词：Yixinnao dripping pills;platelet aggregation;prothrombin time;thrombin time;thrombus formation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effect of Jiangzhi Mixture (IM) on low density lipoprotein (LDL) oxidative susceptibility and low density lipoprotein receptor (LDL-R) gene expression in rats with hyperlipidemia, and elucidate the possible mechanism of IM regulating blood lipid. Method: Sixty Wistar male rats were randomly divided into normal group, model group, simvastatin and IM high, middle, low dose groups, 10 rats in each group. The rats in normal control group were fed with basal diet, the rats in the other groups were fed with a high fat diet to establish hyperlipidemia rat models, and during modeling the drugs were used for prevention. Physiological saline were given to the rats in control group and model group, simvastatin group rats were given 7.2×10-4g·kg-1 simvastatin suspension solution, and Jiangzhi Mixture low, middle, and high dose groups were intragastrically given 0.25, 0.5, 1 g·kg-1 decoction, respectively, 10 mL·kg-1, once a day for 10 weeks, during making model. After 10 weeks, according to the method of abstracting eyeball blood, the blood lipid spectrum of each individual animal in various groups were measured with full automatic biochemical analyzer. After separation of LDL, its susceptibility to oxidation was determinated in vitro. According to the method of Trizol extracting liver RNA of rats, LDL-R mRNA expression was determinated by RT-PCR. Result: Compared with model group, the cholesterol (TC), triglycerides (TG), low density lipoprotein chelesterol(LDL-C), and apolipoprotein B (Apo B) levels in Jiangzhi Mixture groups were regulated and inhibited; the high density lipoprotein chelesterol(HDL-C), apolipoprotein A (Apo A) levels and Apo A/Apo B ratio were increased, meanwhile the differences were statistically significant (P<0.05 or P<0.01); Compared with the model group, the maximum rate of oxidation time (Tmax) and LDL oxidation lag time (lag time) were significantly prolonged, and the relative expression levels of liver LDL-R mRNA were significantly elevated in all Jiangzhi mixture dose groups, and the differences were statistically significant (P<0.05 or P<0.01). Conclusion: Jiangzhi Mixture can modulate blood lipid metabolism in experimental hyperlipidemia rats models, reduce LDL susceptibility to oxidation of hyperlipidemia rat significantly, and induce liver tissue LDL-R mRNA expression of hyperlipidemia rat, and have good adjusting effect on lipid abnormalities.关键词：Jiangzhi Mixture;hyperlipidemia;lipid profile;LDL;LDL-R;gene
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the effects and mechanism of the extract of Ginseng Radix et Rhizoma on hair growth of C57BL/6J mice. Method: This experiment had 5 groups: positive control group (zhangguang 101), blank control group, high dose group (2 g·mL-1), medium dose group (1 g·mL-1), low dose group (0.5 g·mL-1). The skin color and histological examination of hairless mice were investigated to study the effects of promoting hair growth. The skin temperature at different time was measured with infrared radiation thermometers and platelet aggregation rate in vitro was surveyed with nephelometry and chromogenic substrate to preliminarily study the mechanism. Result: All dose groups could induce the hair growth of C57BL/6J mice in a dose-dependent manner from resting stage to growing period, but had no effect on final length of the hair. The differences were significant (P<0.01) between the therapeutic groups and the controls, but no significant between high dose group and positive control group. Histologic examination showed that the extract of Radix Ginseng could produce melanin and induce C57BL/6 mice hair follicle to enter from telogen into anagen. Twenty and sixty minutes after administration, all dose groups can significantly improve the temperature of mice (P<0.01), however, one hundred and twenty minutes later, the temperature recovered to initial time. The blood platelet aggregation induced by platelet activating factor (PAF) at five minutes could significantly and concentration-dependently be inhibited by all dose groups, the platelet aggregation rates of different doses were 49.41%, 27.80%, 18.39%, repevtively, and the differences were significant between the therapeutic groups and the blank control group (P<0.01), but there were no significant differences between the Ginseng Radix et Rhizoma groups and the positive control group. The platelet aggregation activity was partially related to antithrombin and the thrombin inhibition rates of different doses were (20.24±2.72)%, (15.42±1.03)%, (8.61±0.80)%, respectively. The differences were significant between the therapeutic groups and the blank control group (P<0.01). Conclusion: The mechanism of the extract of Ginseng Radix et Rhizoma on hair growth of C57BL/6J could be connected with expending skin capillary and improving local microcirculatory perfusion.关键词：Ginseng Radix et Rhizoma;C57BL/6J mice;growing phase of hair cycle;skin temperature;platelet aggregation;antithrombase
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This experiment is mainly to estimate the expression nuclear factor-kappa B and inflammatory factors related in atherosclerosis (AS) model rats and investigate the anti-AS mechanism of Dahuang Zhechong Pill(DHZCP). Method: SD rats were divided into control group, model group, high and low dose DHZCP treated group(1.4, 0.7 g·kg-1).There are average eight rats every group. AS model was made by high fat feeding and injected vitamin D3.After 21 days, low and high dose DHZCP treated group were given low and high dose DHZCP respectively except control group and model group. Rats were fed totally for 8 weeks, the level of serum total cholesterol (TC), triglyceride (TG), highdensity lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), tumor necrosis factor(TNF-α), pathological change of aortic tissue were observed. Immunohistochemical technique was utilized to measure the expression of nuclear factor κB (NF-κB) and intercellular adhesion molecule-1(ICAM-1). Positive cells rate of NF-κB and ICAM-1 made by half quantitative computer image analysis. Result: Compared with control group, the level of TC, TG, LDL-C, TNF-α in model group were higher and HDL-C was lower remarkably. HE staining revealed the aortic intima was obviously thickened. Cholesterol crystal and fibrous cap were seened in the aortic intima plaque.Immunohistochemical detection showed NF-κB and ICAM-1 protein were found in the intima plaque and plasma membrane of foam cells in model group, While the positive-cells rate of NF-κB and ICAM-1 expression were respectively (47.35±5.18)% and (55.92±0.40)% increased than treatment group(P<0.01, P<0.05). Compared with model group, the level of TC, TG, LDL-C, TNF-α in two treatment group were lower and HDL-C was higher remarkably(P<0.01, P<0.05). Pathological change in high dose DHZCP group is most remarkably decreased than others, While in low dose DHZCP is better than model group. The positive-cells rate of NF-κB and ICAM-1 expression were respectively (16.71±4.43)% and (19.73±0.28)% reduced than model group(P<0.01, P<0.05). Conclusion: DHZCP could have the effect of anti-AS by cut down expression of canonical NF-κB signaling pathway, and inhibition inflammatory factors of TNF-α, ICAM-1.关键词：atherosclerosis;nuclear factor-kappa B;Dahuang Zhechong Pill;tumor necrosis factor;intercellular adhesion molecule-1
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This research was aimed at observing the effect of the Danqi Mixture on the expression of Akt1 in kidney tissue of diabetic rats and exploring it's mechanism of renal protection. Method: Healthy male Sprague-Dawley rats were divided into normal group (A), diabetic model group (B), diabetic group treated with Danqi (C) and Enalapril group (D) randomly. Diabetic rat model was induced using injection of 50 mg·kg-1 streptozotocin(STZ). Rats of each group were killed at the 12th week. Biochemistry assay was employed to assess the urine protein and serum creatinine. The protein expressions of Akt1, E-cadherin and fibronection(FN) in renal tissue were measured using immunohistochemistry, meanwhile α-smooth actin(α-SMA) protein expression level was detected by Western blot. The mRNA level of Akt1 in renal cortex was examined by Reverse Transcriptose Polymerase Chain Reaction(RT-PCR). Result: Danqi mixture and enalapril down-regulated mRNA and protein expression of Akt1, and decreased protein expressions of α-SMA and FN (P<0.01), along with significantly increased level of E-cadherin protein in renal tissue diabetic rats (P<0.01). Comparing to the normal group, the levels of proteinuria, serum creatinine in Danqi Mixture group were remarkably decreased as well (P<0.05). Conclusion: Danqi Mixture could inhibit epithelial-mesenchymal transition(EMT) and improve the injury of diabetic kidney, which might associate with the down-regulation of Akt1 mRNA and protein expression.关键词：diabetic nephropathy;Akt1;epithelial mesenchymal transition;Danqi Mixture;enalapril
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the therapeutic effect in visceral hypersensitivity rat model with irritable bowel syndrome (IBS) induced by acetic acid and analgesic effect of total flavonoids from Alpinia officinarum(GLJ) and supply experimental evidence to the therapy of irritable bowel syndrome. Method: IBS models in SD rats were established by acetic acid for 2 weeks, and then the rats were randomized into model group, pinaverium group, GLJ high dose group, GLJ middle dose group, and GLJ low dose group. After treatment for 2 weeks, the therapeutic effect of GLJ was assessed by observing the abdominal withdrawal reflex (AWR) condition. The analgesic effects of GLJ were observed in pain models of mice induced by hot plate, acetic acid, pressure and formalin. Result: GLJ could effectively reduce the sensitivity of visceral hypersensitivity rat model of IBS induced by acetic acid(P<0.01 or P<0.05) and had analgesic effect on the pain models of hot plate, acetic acid and formalin (P<0.01 or P<0.05). Conclusion: GLJ has better therapeutic effect on visceral hypersensitivity rat model of IBS and analgesic effect.关键词：total flavonoids from Alpinia officinarum;irritable bowel syndrome;visceral hypersensitivity;rat model;analgesic
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effects between Wild and Breeding double teeth mole Faeces Trogopterori on removing blood stasis, anti-inflammatory in rat with acute blood stasis syndrome and paw edema inflammation. Method: Rats were randomly divided into 8 groups for anti-inflammation test: model control group, positive medicine group (aspirin 0.2 g·kg-1), shannxi Faeces Trogopterori of higher, middle and low dosage groups (9, 4.5, 2.25 g·kg-1), wild Faeces Trogopterori of higher, middle and low dosage groups (9, 4.5, 2.25 g·kg-1). All groups were treated continuously for 7 days. Rats were randomly divided into 9 groups for activating-blood: normal control group, model control group, positive group (aspirin 0.05 g·kg-1) and 6 treatment groups which use the same dose and time to the anti-inflammation test. After treatment, carrageenan was injected subcutaneously in the pad of the rat's hindfoot.The volume of the rat's hindfoot was measured at 30 min, 1, 2, 4 h, and 6 h respectively after the modeling, and the degrees of swelling(E%). The indexes of hemorheology including whole blood viscosity under different shear rates, packed cell volume, plasma viscosity, platelet aggregation and fibrinogen were detected for the rat blood stasis syndrome model. Result: All the treatment groups showed the strongest effect after treatment for 1 h. Faeces Trogopterori from Shannxi had the lasting action time. Higher dosage still showed, an obviously decreasing digits swelling degree after 6 h. There was no significant effect of from shaanxi on blood rheology indexes, and whole blood viscosity could be decreased by wild Faeces Trogopterori. Conclusion: The effect of breeding Faeces Trogopterori on anti-inflammatory was better than the wild, but there was no significant effect on removing blood stasis.关键词：Faeces Trogopterori;anti-inflammatory;removing blood stasis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To detect Rho family (RhoA, RhoB, RhoC) mRNA expression and study the role of Rho family in endometriosis. Method: Quantified RT-PCR technique was used in the detection. Result: RhoA and RhoC mRNA expression in endometriosis was lower than that in normal endometrium markedly (P<0.05, vs normal). RhoB mRNA expression didn't show any distinction between the groups. Conclusion: Endometriosis may be related to the low expression of RhoA and RhoC.关键词：endometriosis;Rho family
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the safety of three surfactants from plants, gleditsia saponin, tea saponin and sapindus saponin, in the field of cosmetics. Method: After simple pre-treatment, ethanol used as the extraction agent, 40 mesh-powders from Gleditsia sinensis, tea-seed cake and sapindus mukorossi were extracted respectively. Compared with ammonium alcohol ether sulphat(AESA)which was commonly used in the cosmetics, the 50% hemolytic dosage (HC50), protein denaturation index (DI), and the ratio of HC50/DI(L/D) in hemolysis test were measured to evaluate the safety of leditsia saponin, tea saponin and sapindus saponin. Result: The yield and the real content of sapindus saponin among three surfactants were the lowest. HC50 of gleditsia saponin, tea saponin and sapindus saponin were all lower than 50 mg·L-1, which meant the saponins had stronger irritation than AESA, and the HC50 value of sapindus saponin was the minimum. L/D value of the saponins was less than 1.By eye irritation classification, it showed that three surfactants from plants belonged to the irritating substance. Conclusion: The production cost of sapindus saponin was the highest among three saponins. Considering the strong irritation of the three surfactants from plants, the amount of them should be strictly controlled to decrease the irritation when applied as cosmetics.关键词：surfactants;saponin;hemolysis;the safety of cosmetic raw material
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: The aim of the present study is to identify the toxic substances in Genkwa Flos extract which could induce human liver cell line L02 injury. Method: MTT assay was applied to assess human liver cell line L02 proliferation after incubation with Genkwa Flos extract. The chemical constituents in Genkwa Flos extract and their affinity with L02 cell were measured by ultraperformance liquid chromatography-quadrupole time of flight/mass spectrometry(UPLC-QTOF/MS). Result: Genkwa Flos extract exhibited inhibitive effect on L02 cell in a dose-dependent manner. IC50 value was (48.34±4.66) mg·L-1 after 48 hours incubation with L02 cell. Four flavonoids, such as apigenin, 3'-hydroxygenkwanin, genkwanin, and velutin, were detected in Genkwa Flos extract. Nine diterpene orthoesters were also detected in Genkwa Flos extract, including yuanhuapine, genkwanine L, ynanhuafine, genkwadaphnin, yuanhuatin, yuanhuagine, yuanhuadine, yuanhuajine, and yuanhuacine. The substances in Genkwa Flos extract that had affinity with L02 were 3'-hydroxygenkwanin, genkwanin, velutin, yuanhuapine, genkwadaphnin, yuanhuatin, yuanhuadine, and yuanhuacine. Among these components, yuanhuacine inhibited the L02 proliferation in a dose-dependent manner with the IC50 of (29.57±2.01) mg·L-1 after 48 hours, while genkwanin(0.1-100 mg·L-1) had no markedly toxic activity on L02. Conclusion: Genkwa Flos extract can inhibit the growth of human liver cell line L02 in vitro. Diterpene orthoesters exhibit cellular affinity with L02 cell and show cytotoxicity, indicating that Diterpene orthoesters are the main substances contributing to the toxicity of Genkwa Flos.关键词：Genkwa Flos;human liver cell line L02;toxicity;diterpene orthoesters;yuanhuacine;flavonoids;genkwanin
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate the antiviral therapeutic effects and safety of Bushen Qingtou prescription to hepatitis B virus(HBV) carriers with positive e antigen. Method: With the multi-center, randomized, double-blinded and placebo-controlled methods, 300 cases of HBV carriers with positive e antigen were double-blinded randomized into 2 groups, with 200 cases in treated group and 100 cases in controlled group. During the 52-week treatments, Bushen Qingtou prescription was applied in treated group and Bushen Qingtou placebo prescription was adopted in controlled group. All the drugs above were granular formulation, one dose twice per day taken with water. During the treatments, the DNA quantitative of serum HBV and the two pairs of semi-hepatitis B quantitative were tested and the occurrence of adverse effects was observed. Result: At the 52nd week, the DNA levels of serum HBV in treated group were remarkably decreased, with significant difference compared with that at the 0 week respectively. The ratio of decreasing over 1 lg and over 2 lg was 41.36% and 19.37% in treated group, which were more compared with that of 15.96% and 4.26% in Controlled Group. During the treatments, the average values of HBeAg in treated group were continuously decreasing. At the 52nd week, the average values of HBeAg in treated group were obviously decreased compared with that in the placebo Controlled Group, with significant difference, while the average values of HBsAg in treated group were not better. The ratio of HBsAg decreasing over 0.5 lg was 27.23% in treated group, which was superior to that of 8.51% in Controlled Group, with significant difference. At the 52nd week, in two groups, the decreasing of DNA level of serum HBV over 3 lg and negative-transmission ratio, the HBeAg negative-transmission ratio, the HBeAg seroconversion ratio and the HBsAg decreasing over 1 lg and over 2 lg and negative-transmission ratio were almost the same without significant difference. There was no case of serious adverse effects during the treatments. Conclusion: Bushen Qingtou prescription has the function of HBV inhibition, which have the tendency of time-dependence.关键词：Hepatitis B virus carriers;positive e antigen;Bushen Qingtou prescription;randomized controlled trial
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the efficacy of Chinese herbal formula Sanhuang tang in treating obese type-2 diabetes. Method: Sixty-nine patients conformed to western medicine diagnosis of type 2 diabetes and traditional Chinese medicine's phlegm hot junction card were randomly distributed into Sanhuang tang group and western medicine group.After treatment for 3 months, comparative analysis of glucose and lipid metabolism indicators, indicators of insulin resistance and inflammatory factors. Result: Herbal superior in improving clinical symptoms, glucose and lipid metabolism and insulin resistance index with western medicine group, and reduce the lever of inflammatory factors to some extent. Conclusion: Both western and herbal formula treatment has better efficacy.关键词：Sanhuang Tang;obesity;type 2 diabetes;insulin resistance;inflammatory factors
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Zhengan Xifeng granule on insulin sensitivity, blood pressure, blood glucose and blood lipid in hypertension patients. Method: Sixty-six hyper-tension patients with insulin resistance were randomly divided into treatment group (cooperation of chinese and western medicine group) and control group(western medicine group). Each group was treated for 6 months and then the blood pressure (BP), fasting blood glucose (FBG), total cholesterol (TC), the triglyceride (TG), low-or high-density lipoprotein-cholesterol (LDL-C or HDL-C), free fatty acids, as well as insulin sensitivity index (ISI) were measured. Result: Compared to the control group, the contents of the treatment group were significantly improved as follow:BP decreased(P<0.01), FBG, TC, TG, LDL-C and FFA lowered, HDL-C increased(P<0.05) and the ISl weakened(P<0.05). Conclusion: Zhengan Xifeng granule appeared to decrease blood pressure, stable the blood glucose and blood fat, suppress insulin resistance, enhance the insulin sensitivity as well as modify hyperinsulinemia.关键词：Zhengan Xifeng granule;insulin resistance;hypertension
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of traditional Chinese(TCM) medicine therapy on plasminogen activator(t-PA), plasminogen activator inhibitor(PAI-1) to chronic renal failure(CFR) patients and its intervention way. Method: The patients of chronic kidney disease(CKD) 4 were randomly divided into 4 groups: western medicine group, western medicine with herbs group, western medicine with herbs and clysis group, and western medicine with herbs, clysis and fumigation group, and treated with the medicine respectively.In the third month, serum creatinine, blood urea nitrogen, plasma t-PA and PAI-1 were detected before and after treatment. Result: In TCM treatments, the levels of plasma PAI-1 were decreased, renal function was improved, and the levels of plasma t-PA were increased significantly as compared to western medicine group(P<0.05).The 4th group decreased the levels of serum creatinine, blood urea nitrogen and increased the levels of plasma t-PA bestly(P<0.05), but there were no significant differences among the three groups about the decreasing of plasma PAI-1. Conclusion: TCM treatments could intervent CRF by modulating the activity of fibrinolytic system.关键词：traditional Chinese medicine therapy;chronic renal failure;plasminogen activator;plasminogen activator inhibitor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Response to acute respiratory distress syndrome (ARDS), using the Jiawei Xuan bai Chengqi tang together with western medicine therapy, observation of Jiawei Xuanbai Chengqi tang on patients with mechanical ventilation in ARDS clinical curative effect and its mechanism of action. Method: Thirty-one patients with ARDS, were randomLy divided into the treatment group (16 cases) and control group (15 cases), the control group received mechanical ventilation (tidal volume plus PEEP), treatment group at the same time tube feeding on Jiawei Xuanbai Chengqi tang. Comparison of two groups of patients before treatment and after treatment of 24 hour, 48 hour arterial blood gas indicators: arterial blood pH (pH), oxygen partial pressure (PO2), partial pressure of carbon dioxide (PCO2); ventilatory parameters: peak airway pressure (Ppeek), platform pressure(Pplat), positive end expiratory pressure (PEEP)、oxygen concentration (FiO2); and to compare the duration of mechanical ventilation and ICU time, ARDS causes mortality. Result: The treatment group compared with the control group, 31 cases of patients before treatment of blood gas and ventilation index showed no statistically significant difference; 24 and 48 hours after treatment compared with those before treatment, pH, PO2, PCO2, Parameter improvement, P<0.05; 24 hours and 48 hours after treatment, pH, PO2, PCO2 had no significant difference; Ppeek, Pplat, PEEP, FiO2 treatment after 24 hours, 48 hours were improved after treatment(P<0.05); 24 hours after treatment in two groups showed no statistically significant difference; after treatment for 24 hours and 48 hours comparison, treatment group ventilatory parameters improved indexes(P<0.05); two group of mechanical ventilation time, ICU time and mortality comparison of prognosis, treatment group better(P<0.05). Conclusion: Modified Jiawei Xuanbai Chengqi tang can effectively improve the acute respiratory distress syndrome in patients with mechanical ventilation, ventilation blood gas index. Decrease the duration of mechanical ventilation and retain ICU time, to strengthen the protection of patients with mechanical ventilation in ARDS, improve the patients prognosis.关键词：Jiawei Xuanbai Chengqi tang;acute respiratory distress syndrome;low tidal volume mechanical ventilation
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Bufei addition and subtraction decoction in chronic obstructive pulmonary disease(COPD) a stable condition with the syndrome of qi deficiency of both lung and spleen with detected the expressions of clinical symptoms, physical signs, arterial blood gas, pulmonary function, tumor necrosis factor α(TNF-α), interleukin 8(IL-8). Method: Dividing randomly sixty patients suffering from COPD in a stable condition with the syndrome of qi deficiency of both lung and spleen into 2 groups in even number named as treatment group and control group seperately. Detected the expressions of clinical symptoms, physical signs, arterial blood gas, pulmonary function, tumor necrosis factorα(TNF-α), interleukin 8(IL-8), before and after treatment. Result: After 2 months of treatment, both groups were markedly improve(P<0.05) in clinical symptoms. Pulmonary function had been up (P<0.05), between the Jiajian Bufei decoction after treatment, and the expressions of the TNF-α, IL-8 were lower (P<0.05). The positive-controlled group patients had no obvious adverse reactions after treatment for 2 months. Compared with positive controlled group, the Jiajian Bufei decoction treatment group the expressions of TNF-α, IL-8 were markedly higher(P<0.05). Conclusion: The Yifei Jianpi method can improve the COPD patient's clinical symptoms, postpone the patient 's pulmonary function descend, improve living quality. Lowering content TNF-α, IL-8 into serum.
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the Jiangtangjing granule in effect of treatment for type 2-diabetes and its hypoglycemic mechanism of the initial study. Method :Forty patients with type 2 diabetes randomly divided into Jiangtangjing group and control group were compared by blood sugar, glycated hemoglobin(HbAlc) and traditional Chinese medicine(TCM) symptoms integral change through the eight weeks observation. Result: There was no significant difference between two groups before using the indicators. After treatment, Jiangtangjing- granule group show significant difference in fasting blood sugar, blood sugar, 2 h postprandial glycosylated hemoglobin( HbAlc) compared with before (P<0.05), no statistical difference compared with control group, but the symptoms of Jiangtangjing granule group patients significantly reduced compared with the control group, there was statistically significant (P<0.05). Conclusion: Jiangtangjing granule can improve the glucose metabolism and relieve the symptoms related to TCM of patients with type 2 diabetes.Its mechanism may be related to the level of incretin pancreatic hormone(GLP-1).关键词：Jiangtangjing granule;Type 2-diabetes;glucagon-like peptide 1;sugar metabolism;traditional Chinese medicine symptoms integral
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of treatment on the Qizhi Xueyu type lung cancer of Taohong Siwu soup plus and minus combined with chemotherapy. Method: The 60 cases of Qizhi Xueyu type lung cancer in middle and late period were randomly grouped to control group and treatment group, each group for 30 cases. The control groups were only used western medicine chemotherapy scheme, and the treatment groups were cured together with Taohong Siwu soup based on the treatment of control group. After 3 months' treatment continuously, to observe the short term curative effect, the improvement of life quality, the changes of body weight, the improvement of peripheral blood and immune function, and the occurrence of adverse reaction and the survival rates of patients after 1, 3 and 5 years' treatment. Result: The total effectiveness of control group and treatment group were respectively 90.0% and 70.0%, the short term curative effect of treatment group was better than the control group, and there was significant difference(P<0.05); the improvement rate of life quality was respectively 60.0% and 40.0%, the treatment group was better than the control group, and there was significant difference(P<0.05);the improvement rate of body weight was respectively 16.67% and 16.67%, and there was no significant difference; the peripheral blood abnormality rates of treatment group were obviously lower than the control group, and there was significant difference(P<0.05);the treatment and control group were compared before and after treatment, the immune indexes (CD4+, CD8+, CD4+/CD8+ and the activity of NK cell) all presented significant difference of different degree(P<0.05 and P<0.01), and the immunity of treatment group was obviously improved; compared with control group, the occurrence rate of adverse reaction of treatment group was obviously lower than control group, and there was significant difference(P<0.05). The survival rates of patients after 1, 3 and 5 years' treatment was better than the control group, and there was significant difference(P<0.05). Conclusion: The Qizhi Xueyu type lung cancer was cured with Taohong Siwu soup plus and minus combined with chemotherapy, and it could effectively relieve the clinical symptoms, improve the immunity, reduce the occurrence of adverse reaction, and improve the life quality, and the TCM combined with chemotherapy had the efficacy improved and toxicity lowered, and it was worthy popularized and applied in clinic.关键词：Taohong Siwu soup;chemotherapy;Qizhi Xueyu type;lung cancer;clinical efficacy
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To explore the clinical curative effect on Jiawei Yigan san (JYS) for treatment of infantile fastidium. Method: Eighty-three cases of children with infantile fastidium were randomly divided into the observation group (n=42) and the control group (n=41). The control group was given the pediatric Jianpi Huaji oral liquid (2-3 years old 5-10 mL, tid;>3 years old 10 mL, tid). The observation group was received JYS, 1 dose of tap water decoction (divided in a few portions to take) daily. The treatment lasted for 4 weeks. Serum trace elements Zn and serum neuropeptide Y (NPY) were investigated before and after the treatment. Result: Serum Zn level in the observation group was slightly higher than that in the control group. Serum NPY level in two groups was increased; compared with that before treatment, it was more significant in the observation group (P<0.01). After treatment, the total effective rate in the observation group was 97.62%, higher than that of 82.93% in the control group (P<0.05). After treatment, the score for liver and spleen syndrome in the observation group was (2.5±2.4) which was lower than (7.6±3.2) in the control group (P<0.01). In two groups, body mass index (BMI) after therapy was increased compared with that before treatment (P<0.01), the increase in observation group was higher than that in the control group (P<0.05).After treatment, the total effective rate in the observation group syndrome was 97.62%, which was higher than 80.49% in the control group (P<0.05). Conclusion: Treatment of JYS can increase BMI, food intake and serum NPY level. It has good therapeutic effect for infantile fastidium.关键词：infantile fastidium;Jiawei Yigan san;trace element Zn;serum neuropeptide Y
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Tanreqing injection in treatment of acute lung injury (ALI) on pulmonary function and protective effects on vascular endothelial growth factor effects. Method: One hundred and twenty-four cases of ALI were randomly divided into the observation group and the control group (n=62 each), the control group was received conventional emergency treatment, including venous channel establishment, circulatory support, oxygen supply, antibiotics and primary trauma treatment. On the basis of the measures in the control group, the observation group was given Tanreqing injection (20 mL in 5% glucose intravenous infusion of 250 mL) daily for 7 d. Blood gas indexes, systemic inflammatory response syndrome (SIRS) and Acute physiology and chronic health evaluation II (APACHE II) were investigated. Endothelin-1 (ET-1), vascular endothelial growth factor (VEGF) and von willebrand factor (vWF) were determined. Result: After treatment for 3, 7 days PO2, PCO2 and PO2/FiO2 in observation group were better than those in the control group significantly (P<0.01). After the treatment SIRS and APACHEⅡin observation group were lower than those in control group (P<0.05, P<0.01). After 3, 7 days of treatment, ET-1, VEGF and vWF levels in the observation group were lower than those of the same period accordingly in the control group (P<0.01). Patients who needed mechanical ventilation and with pulmonary infection in observation group were less than that in control group (P<0.05). Conclusion: Tanreqing injection can decrease the serum ET-1, VEGF and vWF levels, reduce endothelial injury, reduce pulmonary edema, so it protects the lung function.关键词：Tanreqing injection;acute lung injury;vascular endothelial growth factor;endothelin-1;von Willebrand factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the effect of salvianolate injection on cardiac function, high sensitivity C reactive protein (hs-CRP), tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in patients with chronic heart failure (CHF). Method: Eighty cases of CHF were randomly divided into the observation group and control group, 40 cases each. The control group was only received western medicine therapy, and the observation group was received western medicine therapy and salvianolate injection (200 mg in 5% glucose injection of 250 mL) iv dropping. The course of treatment of two groups was 14 days. Lee's heart failure score method was used to evaluate cardiac function. The termination and decreasing condition of digoxin was recorded, and hs-CRP, TNF-α and IL-6 were determined. Result: There was obvious difference in effective rate between observation group (95.0%) and control group (77.5%) for heart failure (P<0.05). The LVEF in the observation group (51.3±4.18)% was superior to that in the control group (47.2±4.27)% with a statistical significance (P<0.01). The terminating and decreasing rate of digoxin was 82.5% in the observation group, which was superior to 57.5% in the control group (P<0.05). After treatment, hs-CRP, TNF-α and IL-6 in the observation group were lower than those in the control group (P<0.01). Conclusion: Salvianolate injection can improve cardiac function in the patients with CHF, reduce the dosages of Western medicines, possess good clinical efficacy, resist inflammatory factors (hs-CRP, TNF-α and IL-6), and reduce myocardial injury.关键词：salvianolate;heart failure;cardiac function;hs-CRP;TNF-α;IL-6
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical effects of modified Zhibai Dihuang decoction for patients of systemic lupus erythematosus (SLE) with Yin deficiency. Method: Ninety-eight patients of active SLE with Yin deficiency were randomly divided into two groups.Fifty-two patients in treatment group were treated with modified Zhibai Dihuang Decoction combined with conventional glucocorticoid treatment for 12 weeks, while 46 cases in control group received conventional glucocorticoid treatment, when glucocorticoid showed invalid cyclophosphamide therapy was given. Systemic lupus erythematosus disease activity index (SLEDAI), serum complement (C3 and C4), circulating immunocomplex (CIC) and consumption of hormones were investigated before and after the treatments. Result: After treatment for 8 weeks and 12 weeks, the scores of SLEDAI of treatment group were lower than that in the control group (P<0.05, P<0.01); C3 and C4 value of control group were lower than those before treatment (P<0.05), but those in the treatment group did not change significantly. In both groups, CIC was higher after treatment (P<0.05), the increase in the control group was more significant (P<0.01). The daily and the total consumption of prednisone in the treatment group was less than those in the control group (P<0.05, P<0.01). Conclusion: Combination of modified Zhibai Dihuang decoction with western medicine is more effective compared with western medicine alone for treating active SLE patients with Yin deficiency. The combined therapy can improve the immune system, reduce the amount of glucocorticoids.关键词：systemic lupus erythematosus;modified Zhibai Dihuang decoction;circulating immunocomplex;complement
- 3
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the clinical curative effect of Tianma Gouteng granules (TGG) combined with nifedipine controlled release tablets and captopril for treatment of senile hypertension with hyperactivity of Yang due to deficiency of Yin. Method: Ninety-seven cases of primary hypertension were randomly divided into the treatment group and the control group, 48 cases and 49 cases respectively. Control group was given nifedipine controlled release tablets, 30 mg daily, captopril, 12.5 mg tid orally. The treatment group was received TGG 10 g tid orally plus the western medicines as in the controls.The interventions in two groups were lasted for 12 weeks. Blood pressure controlling rate, changes in urine albumin creatinine ratio (UACR) and Chinese clinical syndromes were investigated. Result: In treatment group, systolic blood pressure (SBP) level was lower than that in control group (P<0.01). The controlling rate for SBP and diastolic blood pressure (DBP) in treatment group was 72.91% (35/48), while 59.18% (29/49) in the control group. The UACR level in treatment group was lower than that in control group (P<0.01). The score of hyperactivity of Yang due to deficiency of Yin in the treatment group was lower than that in the control group. The curative effect of TGG for the score of Chinese medical syndrome was better than that in the control group (P<0.01). Conclusion: TGG combined with captopril and nifedipine controlled release tablets for treatment of senile hypertension with hyperactivity of Yang due to deficiency of Yin, shows high success rate for blood pressure controlling, improvement of clinical symptoms, and certain protection effect on early renal damage induced by hypertension. TGG is worth for spreading use in clinic.关键词：primary hypertension;hyperactivity of Yang due to deficiency of Yin;Tianma Gouteng granules;combined therapy with drugs
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To analysis the clinical effect on enema with traditional Chinese medicine combined with physical therapy for treatment of recurrent pelvic inflammatory disease. Method: One hundred and fifty cases of recurrent pelne cavity inflammation were randomly divided into Chinese medicine enema group, physical therapy group and combination therapy group (n=50 each). The hemorheological changes and the score of life quality were compared. Result: The efficacy of combined treatment group was 98%, obviously superior to 88% of the Chinese medicine enema group and 78% of the physiotherapy group (P<0.05). The hemorheological parameters of combined treatment group were obviously superior to those of the Chinese medicine enema group/physical therapy group (P<0.05). The physical function, mental function, social function, cognitive functioning and overall score of life quality in the combined treatment group were significantly superior to those in the Chinese medicine enema group/physical therapy group (P<0.05). Conclusion: The enema of traditional Chinese medicine combined with physiotherapy can significantly improve treatment outcomes of recurrent pelvic inflammatory disease, improve hemorheological parameters and the quality of life.关键词：enema with traditional Chinese medicine;physical therapy;recurrent pelvic inflammatory disease;hemorheology;quality of life
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To describe the efficacy of Modified Xuefu Zhuyu decoction (MXZD) on patients with acute pulmonary contusion and its effect on endothelin (ET-1), interlukin-8 (IL-8) and C-reactive protein (CRP). Method: Eighty-four patients with acute pulmonary contusion were divided into two groups randomly: observation group and control group (n=42 each). In control group, the patients were treated only with routine therapy. In observation group, they were prescribed with MXZD (one dose daily). Clinical symptom, blood gas, mechanical ventilation rate, lung infection rate, acute respiratory distress syndrome (ARDS) rate, death rates and serum ET-1, IL-8 and CRP were observed and analyzed. Result: After treatment, the clinical stages of the observation group were lower than that of control group (P<0.05). PO2, PCO2 and PO2/FiO2 in the observation group were significantly higher than those in control group (P<0.05, P<0.01). Compared with the control group, the mechanical ventilation rate and lung infection rate in the observation group were lower without a statistical significance, while ET-1, IL-8 and CRP in the observation group were significantly lower (P<0.05, P<0.01). Conclusion: MXZD can diminish clinical symptoms of acute pulmonary contusion, decrease plasma ET-1, IL-8 and CRP, increase PO2, PCO2 and PO2/FiO2, and improve the recovery of pulmonary function caused by pulmonary contusion.关键词：pulmonary contusion;Modified Xuefu Zhuyu decoction;endothelin-1;interleukin-8;C-reactive protein
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical efficacy of Danbie capsule combined with mifepristone in treatment of uterine fibroids. Method: Sixty-eight patients with uterine fibroids were randomly divided into observation group and control group (n=34 each). The control group was orally given 12.5 mg of mifepristone, qd; except mifepristone, the observation group was given Danbie capsule, 5 capsules tid for 12 weeks. The fibroid volume was observed by B-ultrasound, sex hormone levels and qi stagnation and blood stasis scores were also investigated. Result: After treatment, the uterine fibroids volume in the observation group was reduced by 68.7% than before while the control group reduced by 60.6% than before, and the average fibroids volume in the observation group was less than that in the control group (P<0.05); the serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen 2 (E2) and progestin (P) levels had decreased than those before, and the FSH, LH, E2 in the observation group were lower than those in the control group (P<0.05 or P<0.01); after treatment, qi stagnation and blood stasis scores of the observation group was (5.9±2.87), lower than (9.4±3.69) of the control group (P<0.01);the clinical efficacy of the observation group was better than the control group (P<0.05). Conclusion: Danbie capsule combined with mifepristone for treating uterine fibroids is effective and it can significantly reduce FSH, LH and E2 level, reduce the symptoms, improve clinical outcomes, and it has significant short-term effect.关键词：uterine fibroids;Danbie capsules;Mifepristone;promote blood circulation to remove blood stasis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To observe the clinical effect of Shujie Tuire decoction for treating pediatric exogenous fever. Method: One hundred cases were divided into two groups randomly. Fifty cases in the treatment group were given Shujie Tuire decoction, besides, 50 cases in the control group were given acetaminophen suspension fluid. Finally, antifebrile effect in the two groups were observed and compared. Result: The effective rate of the treatment group was higher than that in the control group, besides, the defervescence time and blood test results of the treatment group were better than the control group. The differences in the above observations were statistically significant, (P<0.05). Conclusion: Shujie Tuire decoction treatment for exogenous fever is effective and safe.关键词：pediatric exogenous fever;Shujie Tuire decoction;curative effect
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To study the influence of Fuzheng Shugan Xiaoai decoction (FSXD) on relapse, metastasis and life quality in breast cancer patients with postoperative radiation and chemotherapy. Method: Eighty-two cases of breast cancer patients after modified radical mastectomy were randomly divided into the observation group (n=43) and the control group (n=39). The control group was given routine western medicine symptomatic treatment. Except the routine symptomatic treatment, the observation group was given FSXD, 1 dose daily right after radiotherapy or/and chemotherapy until 3 months after the ending of those therapies. Afterwards, 4-7 doses of FSXD per week were given to a patient for 1 year. Generally, a dose of FSXD was decocted and divided in half for 2 services daily. When decoction was inconvenient for some patients, the formulated granules were given. The life quality, the main symptoms and signs, relapse and metastasis were recorded. Vascular endothelial growth factor (VEGF) was determined for the patients. Result: After 6, 12 months of treatment, the overall health scores in the observation group were higher than those in the control group (P<0.01). After 6, 12 months of treatment, the overall life quality in the observation group was higher than that in the control group (P<0.01). After the ending of radiotherapy and chemotherapy, additional treatment of FXSD for 6, 12 months the main symptoms and signs in observation group were lower than those in the control group (P<0.01). The serum VEGF levels in the observation group after treatment of 6 and 12 months were lower than those in the control group, the difference was significant (P<0.05, P<0.01). Conclusion: FSXD can reduce the main symptoms and signs for breast cancer patients with postoperative radiotherapy and chemotherapy, improve the quality of life for the patients, and reduce serum VEGF level. Therefore, FSXD may prevent the recurrence and metastasis of the cancer.关键词：Fuzheng Shugan Xiaoai decoction;breast cancer;postoperative;life quality;relapse and metastasis;vascular endothelial growth factor
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To evaluate the therapeutic effects of Qingre Sanwen oral liquid on children with hand, foot and mouth disease (HFMD). Method: Ninety-four patients with HFMD in our out-patient department were randomly divided into two groups. Forty-eight patients were treated with Qingre Sanwen oral liquid(5-10 mL, tid), forty-six patients were treated with ribavirin (10-15 mg·kg-1·d-1). The treatment lasted for 3 days. Result: The marked effect rates were 63.82% and 56.81% in the observation group and in the controls accordingly without significant difference. The total effect rates were 93.64% and 86.36% in the observation group and in the controls respectively without significant difference. The observation group showed no adverse reaction. The control group had diarrhea in four cases and vomiting in 3 cases. Conclusion: Qingre Sanwen oral liquid has a good therapeutic effect on children with HFMD, and no adverse reactions.关键词：hand, foot and mouth disease;Qingre Sanwen oral liquid;children
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: By analyzing and mining these obtained numerous pharmacodynamic test data, finally testify the characteristic of two-way applicable of Chinese herbs with neutral property. Method: Weka analysis software and bayesian network analysis method were used in this research. The procedure was data preprocessing, attribute selection, building related Bayesian network model, classification forecasting. Result: In the heat stagnation and blood stasis syndrome, AUC values of classification between three different herb property groups and model control group were in order of neutral property herb>cold property herb>hot property herb.But in the cold stagnation and blood stasis syndrome, the order was hot property herb>neutral property herb>cold property herb. Conclusion: To treat heat stagnation and blood stasis syndrome, the effect of neutral property herb and cold property herb were better than hot property herb, even neutral property herb was better than cold property herb.To treat cold stagnation and blood stasis syndrome, the effect of neutral property herb and hot property herb were better than cold property herb.These results proved that neutral property herbs were two-way applied to cold and hot syndrome, which did not rely on concocted degeneration or compatibility.关键词：neutral property herbs;herb property;two-way application;bayesian analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: This paper aims to build a prescription information database, mining the rules of drugs in the prescription, and study the compatibility law. Method: Using information extraction based on regular expressions and vs 2008 programming to realize automatic extraction, and realized association rules mining in SQL Sever 2005. Result: Generated a structured prescription information database, and initial extracted prescription information 81 305.When the support degree is 10% and the confidence level is 70%, 9 kinds of high frequency drus and 4 rules were geted. Conclution: The method of information extraction based on regular expressions make it be possible that make a full-scale study of prescription compatibility law. The results of data mining and the theory of traditional Chinese medicine and clinical experience were matching overall, which lay a foundation for further study.关键词：association rules;prescription compatibility;information extraction
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: Using evidence-based Meta analysis to evaluate traditional Chinese medicine enema method in the treatment efficacy and safety of chronic renal failure. Method: Retrieval of domestic medical journals, choose Chinese medicine enema method with oxyamyli tectus aldehydum in the treatment of chronic renal failure randomized controlled experimental study.8 randomized controlled clinical trials and Meta analysis. Result: Compared with oxyamyli tectus aldehydum, enema method in the treatment of chronic renal failure in overall efficacy, lower serum creatinine, blood urea nitrogen, are superior to that of oxyamyli tectus aldehydum group. Conclusion: Enema method in treating chronic renal failure of the superior efficacy of oxyamyli tectus aldehydum, but also needs to be well designed randomized controlled multicenter clinical trial and further confirmed.关键词：evidence-based medicine;enema method;Meta analysis
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To review the research on monomer components of Chinese materia medica for immunoregulation of chronic hepatitis B. Method: The articles related to monomer components of Chinese materia medica for immunoregulation of chronic hepatitis B in CNKI database and PubMed Database from January 2000 to September 2012 were retrieved with the key words of'Chronic hepatitis B, monomer components of Chinese materia medica, mmunoregulation'. A total of 126 literatures were obtained from computer screen, and 45 documents of them were involved for summarization. Result: A lot of monomer components of Chinese materia medica has been screened, for example, root of whitebackleaf mallotus, astragaloside IV, homobarringtonie. The mechanisms is closely related with inhibiting HBVDNA replication, regulating the balance of Thl/Th2 cell factors, promoting the maturation of dendritic cell, regulating the express of Toll-like receptor, regulating the singal of hepatocyte apoptosis. Conclusion: The monomer components of Chinese materia medica play an key role in immunoregulation of chronic hepatitis B.But there are some problem need to be solved, for example, the control of herb resource and preparation, the lack of study on evidence based medicine in large sample and long term effect research. It is heavy responsibility to deal with these problems.关键词：chronic hepatitis B;monomer components of Chinese materia medica;immunoregulation
- 3
- |
- 3
- |
- 0
发布时间：2024-01-04 - 摘要：Objective: To determine the impact of supplementing Qi and nourishing Yin on the rate of body weight in patients with lung cancer. Method: The articles about supplementing Qi and nourishing Yin and lung cancer were collected in CBM, CNKI, Weipu from 2002 to 2012. Result: A total of 91 literatures were searched, and 5 studies met the inclusion criteria were identified for meta-analysis. In comparison to control groups, patients who received supplementing Qi and nourishing Yin had a better weight change. Conclusion: The use of supplementing Qi and nourishing Yin played a significant role in lung cancer and improved body weight and the quality of life.关键词：R project;Meta-analysis;supplementing Qi and nourishing Yin;lung cancer;Chinese medical treatment
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：To summarize the progress on pharmacological activities through the analysis of literature on plants of Oxytropis. It is helpful for the researchers to carry out further researches on the genus of Oxytropis plants in the future. This paper reviewed the researches of various pharmacological activities in Oxytropis. O. glacialis has antimicrobial activity. O. glabra and O. Kansuensis have significant antitumor effect. While O. falcata shows prominent activities of anti-inflammatory, analgesia, antitumor and antiviral. Studies on plants of the genus of Oxytropis will have important significance on finding new bioactive compounds.关键词：Oxytropis;pharmacological activities;antitumor;anti-inflammatory and analgesia
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04 - 摘要：To promote the application of sour-sweet herbs for nourishing Yin, such as Shaoyao Gancao decoction (SGD) in treating pediatric diseases, the author summarized typical medical records that use SGD and its modified receipts based on syndrome differentiation. Cases included pediatric intestinal spasm, Tourette syndrome, bronchial asthma, and children night crying. The results showed that although the symptoms of children with Tourette Syndrome were different from those with night crying, the pathogenesis of both diseases showed common features. These features are 'Yang in the liver is often in excess' and 'Yang is frequently in excess while Yin is often in deficiency'. SGD contains sour-sweet herbs which can nourish Yin, and harmonize liver and spleen. It functions in a way to nourish blood and Yin, suppress hyperactive liver and wood, astringe and soften liver. The use of SGD targeted the pathogenesis of both diseases and therefore achieved a satisfactory effect. The use of SGD for pediatric intestinal spasm, and be used at the time when wheeze is widely presented in the lung in children with bronchial asthma, is based on syndrome differentiation as well as modern pharmacological research findings about SGD's antispasmodic, analgesic, anti-inflammatory, cough-relieving, and anti-allergic effects. This suggests prescriptions selected based on traditional Chinese medicine theories and modern pharmacology research findings can serve multiple purposes and improve clinical outcomes.关键词：sour-sweet herbs for nourishing Yin;Shaoyao Gancao decoction;pediatric disease;experience summary
- 2
- |
- 2
- |
- 0
发布时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0