最新刊期
卷 16 ， 期 11 ， 2010
- 摘要：Objective:Research the best technology of Shuanghuanglian Effervescent Tablets by dry granulation.Method:Inspect the influence of roller press,roller speed and the water content of extract powder on yield and friability of particle diameter.Result:The roller press,roller speed and the water content of extract powder had a significant degree of influence.Conclusion:The investigation of Shuanghuanglian Effervescent Tablets by dry granulation would provide the reference technology for study and production of Shuanghuanglian Effervescent Tablets.关键词：Shuanghuanglian Effervescent Tablets;dry granulation;the roller press;roller speed;the water content of extract powder
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发布时间：2024-09-23 - 摘要：Objective: Research the best technology of Shuanghuanglian Effervescent Tablets by dry granulation. Method: Inspect the influence of roller press, roller speed and the water content of extract powder on yield and friability of particle diameter. Result: The roller press, roller speed and the water content of extract powder had a significant degree of influence. Conclusion: The investigation of Shuanghuanglian Effervescent Tablets by dry granulation would provide the reference technology for study and production of Shuanghuanglian Effervescent Tablets.关键词：Shuanghuanglian Effervescent Tablets;dry granulation;the roller press;roller speed;the water content of extract powder
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发布时间：2024-09-23 - 摘要：Ganoderma applanatum was extracted with fast magnetic sound generator. Response surface analysis(RSA) with orthogonal experimental design was successfully applied to determine the optimal conditions where the maximum extraction of the G. applanatum with fast magnetic sound generator occurred, which was at ultrasonic intensity 26.6 KHz, magnetic strength 1 252.5 GS, extraction time 40 min and the concentration of ethanol 70%. In this extraction conditions, the yield of extract can reach 11.86%. This study shows that traditional Chinese medicine fast magnetic sound generator is a very effective experimental device for rapid extraction of Chinese medicine.关键词：Ganoderma applanatum;s ound and magnetic generator;RSA
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发布时间：2024-09-23 - 摘要：Ganoderma applanatum was extracted with fast magnetic sound generator.Response surface analysis（RSA） with orthogonal experimental design was successfully applied to determine the optimal conditions where the maximum extraction of the G.applanatum with fast magnetic sound generator occurred,which was at ultrasonic intensity 26.6 KHz,magnetic strength 1 252.5 GS,extraction time 40 min and the concentration of ethanol 70%.In this extraction conditions,the yield of extract can reach 11.86%.This study shows that traditional Chinese medicine fast magnetic sound generator is a very effective experimental device for rapid extraction of Chinese medicine.关键词：Ganoderma applanatum;sound and magnetic generator;RSA
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发布时间：2024-09-23 - 摘要：目的:优选膏方煎煮工艺的最佳条件参数。方法:以出膏率为指标,煎煮次数、煎煮时间、加液料比为3因素,用L9（34）正交试验优选出膏方的最佳煎煮工艺参数。结果:经优选的工艺条件为煎煮3次,第1次加水量为7倍,第2,3次加水量为5倍,每次煎煮时间为1 h。结论:该工艺合理,出膏率高。关键词：膏方;煎煮工艺;正交试验;出膏率
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发布时间：2024-09-23 - 摘要：Objective: To establish the optimal extraction technology of forsythiaside by orthogonal test. Method: The amount of water, extraction time, extraction times and extraction temperature were set as index for orthogonal test. Result: The factors were: C > A> B> D . Extraction times ( C ) was as the main factor and the amount of water ( A ), extraction time ( B ), extraction temperature ( D ) as a secondary factor. The optimum conditions for extraction was as follows: 16 times of water, soaking extraction 3 times at 60 ℃, each time extracting for 1 hour. Conclusion: The optimum extraction process for forsythiaside was simple, stable and feasible.关键词：forsythiaside;extraction process;orthogonal test
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发布时间：2024-09-23 - 摘要：Objective:To establish the optimal extraction technology of forsythiaside by orthogonal test.Method:The amount of water,extraction time,extraction times and extraction temperature were set as index for orthogonal test.Result:The factors were:C > A > B > D.Extraction times（C） was as the main factor and the amount of water（A）,extraction time（B）,extraction temperature（D） as a secondary factor.The optimum conditions for extraction was as follows:16 times of water,soaking extraction 3 times at 60 ℃,each time extracting for 1 hour.Conclusion:The optimum extraction process for forsythiaside was simple,stable and feasible.关键词：forsythiaside;extraction process;orthogonal test
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- 490
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发布时间：2024-09-23 - 摘要：目的:优选调脂片成型工艺。方法:以制粒和压片过程的工艺参数为评价指标,选择适宜的辅料及其用量。结果:最优成型工艺条件为:干膏粉、淀粉、乳糖的配比为7∶1.5∶1.5,用1.5%PVP乙醇液作为黏合剂制湿粒（用量约为粉料总量25%）,加入1%的CMS-Na为崩解剂,0.7%的硬脂酸镁和0.5%的微粉硅胶分别作为润滑剂与助流剂。结论:该成型工艺可用于调脂片的工业生产。关键词：调脂片;成型工艺
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发布时间：2024-09-23 -
Optimization of Extraction Technology for Shaoshangling Film with Multiple Guidelines Grading Method
摘要：Objective: To optimize the preparation process for Shaoshangling Film by alcohol.Method: The contents of emodin and berberine hydrochloride and the yield of extract were used as index of appraisal. Data processing was carried out with the multiple guidelines grading method for optimizing the extraction condition. The orthogonal experimental design was introduced to optimize the conditions of the alcohol extraction technology. The experiment were involved four factors, concentration of alcohol, amount of alcohol, refluxing time, times of refluxing.Result: The best extraction technology was that refluxing 3 times, refluxing 2 hours each time and in 6 times amount of 95% alcohol.Conclusion: The process is simple, reasonable and relialbe.关键词：Shaoshangling Film;emodin;berberine hydrochloride;orthogonal test;grading methods- 0
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发布时间：2024-09-23 -
Optimization of Extraction Technology for Shaoshangling Film with Multiple Guidelines Grading Method
摘要：Objective:To optimize the preparation process for Shaoshangling Film by alcohol.Method:The contents of emodin and berberine hydrochloride and the yield of extract were used as index of appraisal.Data processing was carried out with the multiple guidelines grading method for optimizing the extraction condition.The orthogonal experimental design was introduced to optimize the conditions of the alcohol extraction technology.The experiment were involved four factors,concentration of alcohol,amount of alcohol,refluxing time,times of refluxing.Result:The best extraction technology was that refluxing 3 times,refluxing 2 hours each time and in 6 times amount of 95% alcohol.Conclusion:The process is simple,reasonable and relialbe.关键词：Shaoshangling Film;emodin;berberine hydrochloride;orthogonal test;grading methods- 0
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发布时间：2024-09-23 - 摘要：Objective:To optimize the best softening and cutting technology of Phellodendron chinense.Method:The content of berberian and alcohol-soluble extract was regarded as evaluated index to determine the best softening and cutting technology of P.chinense.with different ways.Result:Spraying was best softening method that Phellodendron was sprayed at the temperature of 20 ℃ in 8 hours with the total water of 150 mL per 100 g Phellodendron.The reasonable cutting method was to cut the Phellodendron perpendicular to the fiber in 4 mm width.Conclusion:Softening and cutting were reasonable with perfect stability that could be applied to the industrial manufacture and the quality control of Phellodendron chinense Schneid.
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发布时间：2024-09-23 - 摘要：Objective: To optimize the best softening and cutting technology of Phellodendron chinense . Method: The content of berberian and alcohol-soluble extract was regarded as evaluated index to determine the best softening and cutting technology of P. chinense . with different ways. Result : Spraying was best softening method that Phellodendron was sprayed at the temperature of 20 ℃ in 8 hours with the total water of 150 mL per 100 g Phellodendron. The reasonable cutting method was to cut the Phellodendron perpendicular to the fiber in 4 mm width. Conclusion: Softening and cutting were reasonable with perfect stability that could be applied to the industrial manufacture and the quality control of Phellodendron chinense Schneid.
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发布时间：2024-09-23 - 摘要：Objective: To optimize the extraction procedure of Wuwei Weikang Capsule. Method: The optimal extraction process of the Wuwei Weikang Capsule was studied by orthogonal design with extractive yield and the content of paeoniflorin as index. Result: The optimal procedure was using the circulation reflux extracted for 2 times with 8 times 60% ethanol, 1.5 hours each time. Conclusion: The method was effective for the extraction of active components in Wuwei Weikang Capsule and could provide tab data for industrial production of Wuwei Weikang Capsule.关键词：Wuwei Weikang Capsule;Extracting Technology;paeoniflorin
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发布时间：2024-09-23 - 摘要：Objective:To optimize the extraction procedure of Wuwei Weikang Capsule.Method:The optimal extraction process of the Wuwei Weikang Capsule was studied by orthogonal design with extractive yield and the content of paeoniflorin as index.Result:The optimal procedure was using the circulation reflux extracted for 2 times with 8 times 60% ethanol,1.5 hours each time.Conclusion:The method was effective for the extraction of active components in Wuwei Weikang Capsule and could provide tab data for industrial production of Wuwei Weikang Capsule.关键词：Wuwei Weikang Capsule;Extracting Technology;paeoniflorin
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发布时间：2024-09-23 - 摘要：Objective: To establish an effective method for simultaneously determining the concentration of brucine and strychnine in rat plasma by HPLC. Method: The processed rat plasma samples were separated on a Kromasil C18 column. The mobile phase consisted of acetonitrile-0.01 mol ·L-1 sodium heptane sulfonate and 0.02 mol ·L-1 potassium dihydrogen phosphate,adjusted pH to 2.8 with 10% phosphonic acid (24 ∶76). The detection wavelength was set at 264 nm. Result: Chromatograms showed endogenous peaks not interfering with the analysis. The calibration curves of brucine and strychnine were both in good linearity within the range of 50-2 000 μg ·L-1. The mean extraction recoveries were more than 86% while the precisions were less than 8%. The results of stability experiment meet certain requirements for the biopharmaceutical analysis. Conclusion: The method with good sensitivity and precision is applicable to the pharmacokinetic studies of the main compounds in total alkaloids from seeds of Strychnou nux-vomica .关键词：brucine;strychnine;biopharmaceutical analysis;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To establish an effective method for simultaneously determining the concentration of brucine and strychnine in rat plasma by HPLC.Method:The processed rat plasma samples were separated on a Kromasil C18 column.The mobile phase consisted of acetonitrile-0.01 mol.L-1 sodium heptane sulfonate and 0.02 mol.L-1 potassium dihydrogen phosphate,adjusted pH to 2.8 with 10% phosphonic acid（24∶ 76）.The detection wavelength was set at 264 nm.Result:Chromatograms showed endogenous peaks not interfering with the analysis.The calibration curves of brucine and strychnine were both in good linearity within the range of 50-2 000 μg.L-1.The mean extraction recoveries were more than 86% while the precisions were less than 8%.The results of stability experiment meet certain requirements for the biopharmaceutical analysis.Conclusion:The method with good sensitivity and precision is applicable to the pharmacokinetic studies of the main compounds in total alkaloids from seeds of Strychnou nux-vomica.关键词：brucine;strychnine;biopharmaceutical analysis;HPLC
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发布时间：2024-09-23 - 摘要：目的:建立天南星中的草酸钙针晶的含量测定方法。方法:采用HPLC法,Diamonsil C18柱（4.6 mm×250 mm,5μm）色谱柱;流动相0.5%KH2PO4-H3PO4水溶液（pH 2.3）,流速0.7 L.min-1;检测波长210 nm;柱温28℃。结果:草酸在0.06～1.20μg线性关系良好（r=0.999 9）。平均回收率为100.89%（n=5）,RSD为1.07%。结论:本法快速、灵敏、准确、可靠、重复性好,可用于天南星草酸钙针晶定量研究。关键词：天南星;HPLC;草酸钙针晶
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发布时间：2024-09-23 - 摘要：Objective:To establish the quality standards of Xuefu Chuanping gel patch.Method:Semen Sinapis.Corydalis and Herba Asari were identified by thin layer chromatography（TLC）.The content of ephedrina hydrochloridum was determined by high performance liquid chromatography（HPLC）.Result:Semen Sinapis.Corydalis and Herba Asari were identified by thin layer chromatography（TLC）.The characteristic identification was distinct and highly specific.Ephedrina hydrochloridum was linear in the range of 0.032 ～ 0.096 μg.Average recovery was 98.12%,RSD was 1.08%.The content of ephedrina hydrochloridum in gels was 4.25 mg.g-1.Conclusion:The quality control method is reliable,accurate and specific.It can be used for quality control of Xuefu Chuanping gel patch.关键词：Xuefu Chuanping Gel Patch;quality standards;HPLC
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发布时间：2024-09-23 - 摘要：Objective: To establish the quality standards of Xuefu Chuanping gel patch. Method: Semen Sinapis. Corydalis and Herba Asari were identified by thin layer chromatography(TLC). The content of ephedrina hydrochloridum was determined by high performance liquid chromatography(HPLC). Result: Semen Sinapis. Corydalis and Herba Asari were identified by thin layer chromatography(TLC). The characteristic identification was distinct and highly specific. Ephedrina hydrochloridum was linear in the range of 0.032～0.096 μg. Average recovery was 98.12%,RSD was 1.08%. The content of ephedrina hydrochloridum in gels was 4.25 mg ·g-1. Conclusion: The quality control method is reliable, accurate and specific. It can be used for quality control of Xuefu Chuanping gel patch.关键词：Xuefu Chuanping Gel Patch;quality standards;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To study the absorption and accumulation of microelements among different strains of Pinellia ternate grown in Jingzhou.Method:Microelements in powders of leaves and tubers of four different strains were detected with the technique of wavelength dispersive X-ray fluorescence（WDXRF）.Result:Eighteen microelements（K,Ca,Cl,S,P,Mg,Na,Si,Al,Fe,Zn,Mn,Sr,Cu,Cr,Ni,Se and Ti） have been detected,but Se was not found in leaves and Ti not in tuber.Conclusion:The absorption and accumulation of microelements were different among different strains of P.ternate grown in Jingzhou.And wether might this cause any differences in efficacy and compatibility needs to be further studied.关键词：Pinellia ternate grown in Jingzhou;strains;microelement
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发布时间：2024-09-23 - 摘要：Objective: To study the absorption and accumulation of microelements among different strains of Pinellia ternate grown in Jingzhou. Method: Microelements in powders of leaves and tubers of four different strains were detected with the technique of wavelength dispersive X-ray fluorescence (WDXRF). Result: Eighteen microelements (K, Ca, Cl, S, P, Mg, Na, Si, Al, Fe, Zn, Mn, Sr, Cu, Cr, Ni, Se and Ti) have been detected, but Se was not found in leaves and Ti not in tuber. Conclusion: The absorption and accumulation of microelements were different among different strains of P. ternate grown in Jingzhou. And wether might this cause any differences in efficacy and compatibility needs to be further studied.关键词：Pinellia ternate grown in Jingzhou;strains;microelement
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发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents of Panax ginseng.Method :The compounds were separated and purified by HPLC and their structures were conformed by TLC and NMR.Result:The structures of eight compounds were conformed,they were 20（S）-protopanaxadiol（1）,ginsenoside-Rc（2）,20（S）-ginsenoside-Rg2（3）,20（S）-ginsenoside-Rg3（4）,20（S）-ginseno side-Rh1（5）,ginsenoside-Rb3（6）,20（S）-protopanaxatriol（7）,ginsenoside-Rf（8）.Conclusion:Compound 1,compound 7 and compound 8 were the first found in P.ginseng.This research provided theoretical evidences for the far-reaching use of P.ginseng.关键词：Panax ginseng;ginsenoside;chemical constituents;isolation and identification
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发布时间：2024-09-23 - 摘要：Objective: To study the chemical constituents of Panax ginseng . Method :The compounds were separated and purified by HPLC and their structures were conformed by TLC and NMR. Result: The structures of eight compounds were conformed, they were 20(S)-protopanaxadiol(1), ginsenoside-Rc(2), 20(S)-ginsenoside-Rg2(3), 20(S)-ginsenoside-Rg3(4), 20(S)-ginseno side-Rh1(5), ginsenoside-Rb3(6), 20(S)-protopanaxatriol(7), ginsenoside-Rf(8). Conclusion: Compound 1, compound 7 and compound 8 were the first found in P. ginseng . This research provided theoretical evidences for the far-reaching use of P. ginseng .关键词：Panax ginseng;ginsenoside;chemical constituents;isolation and identification
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发布时间：2024-09-23 - 摘要：Objecive:To discuss the relationship between water-soluble sugar and cold-heat properties and further reveal correlations of four properties and material basis.Method:Water-soluble sugars were purified from 10 herbs with cold properties and 10 herbs with heat properties.After acetylation,GC /MS fingerprints of water-soluble sugars were determined and the results were analyzed by Fisher.Result:Discriminant function had been established by Fisher.The Fisher discriminant analysis could distinguish the cold and heat properties between 20 herbs that the back substitution in group was 100%.There was difference between the drugs with cold and heat properties in the GC /MS fingerprints,which corresponded to the different water-soluble sugars in the cold and heat herbs.Conclusion:The Fisher analysis could differentiate the herbs with cold and heat properties,and it was an effective statistical tool to study the relationship between the cold and heat properties by water-soluble sugar.It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.关键词：water-soluble sugar;cold and heat properties;GC /MS fingerprints;Fisher analysis
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发布时间：2024-09-23 - 摘要：Objecive: To discuss the relationship between water-soluble sugar and cold-heat properties and further reveal correlations of four properties and material basis. Method: Water-soluble sugars were purified from 10 herbs with cold properties and 10 herbs with heat properties. After acetylation, GC/MS fingerprints of water-soluble sugars were determined and the results were analyzed by Fisher. Result: Discriminant function had been established by Fisher. The Fisher discriminant analysis could distinguish the cold and heat properties between 20 herbs that the back substitution in group was 100%. There was difference between the drugs with cold and heat properties in the GC/MS fingerprints, which corresponded to the different water-soluble sugars in the cold and heat herbs. Conclusion: The Fisher analysis could differentiate the herbs with cold and heat properties, and it was an effective statistical tool to study the relationship between the cold and heat properties by water-soluble sugar. It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.关键词：water-soluble sugar;cold and heat properties;GC/MS fingerprints;Fisher analysis
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发布时间：2024-09-23 - 摘要：Objective:To optimize the technological conditions for effective compounds of Aucklandia lappa.by microwave-assisted extraction.Method:The microwave extract technology was chosen and optimized by an orthogonal test.The contents of costunolide and dehydrocostuslactone were determined by HPLC.Each influence factor of effective component of A.lappa by the microwave-assisted extraction was observed.Result:The best extraction was obtained with 400 W as the microwave power.Four volume of ethyl alcohol（60%） extracted for four times and each extract last 20 minitues.Conclusion:The microwave extraction technique is bettev than backflow extraction method.with high efficiency and short extraction time.关键词：Aucklandia lappa;costunolide;dehydrocostuslactone;microwave-assisted extraction
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发布时间：2024-09-23 - 摘要：Objective: To optimize the technological conditions for effective compounds of Aucklandia lappa. by microwave-assisted extraction. Method: The microwave extract technology was chosen and optimized by an orthogonal test. The contents of costunolide and dehydrocostuslactone were determined by HPLC. Each influence factor of effective component of A. lappa by the microwave-assisted extraction was observed. Result: The best extraction was obtained with 400 W as the microwave power. Four volume of ethyl alcohol (60%) extracted for four times and each extract last 20 minitues. Conclusion: The microwave extraction technique is bettev than backflow extraction method. with high efficiency and short extraction time.关键词：Aucklandia lappa;costunolide;dehydrocostuslactone;microwave-assisted extraction
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发布时间：2024-09-23 - 摘要：目的:建立HPLC法测定降糖明目颗粒中羟基红花黄色素A的含量测定方法。方法:采用Waters symmetry色谱柱（4.6 mm×150 mm,5μm）;以水-三氟乙酸（100∶0.1）为A相,乙腈-三氟乙酸（100∶0.1）为B相,梯度洗脱程序为0～20min,5%～20%（B）;流速为1.0 mL.min-1;检测波长为403 nm;柱温40℃。结果:羟基红花黄色素A在0.099 2～3.968μg呈良好线性关系,r=0.999 9,平均回收率和RSD分别为100.2%和2.96%。结论:本方法简便、准确、重复性好,可测定该制剂中羟基红花黄色素A的含量。关键词：高效液相色谱法;降糖明目颗粒;红花;羟基红花黄色素A
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发布时间：2024-09-23 - 摘要：Objective:To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes.Method:The HPLC analysis was carried out on a ELITE C18（4.6 mm × 250 mm,5 μm） column with mobile phase consisted of acetonitrile-0.06 mol.L-1 KDP buffer（38∶ 62） at a flow rate of 1.0 mL.min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination.Result:The 1inear range of vinorelbine was 2.08 ～ 20.8 μg（r = 0.999 9）.The average recovery was 98.64%（RSD 1.09%）.The average entrapment efficiency was 86.83%.Conclusion:This method is simple,precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.关键词：vinorelbine;liposome;content determination;entrapment efficiency
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发布时间：2024-09-23 - 摘要：Objective: To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes. Method: The HPLC analysis was carried out on a ELITE C18 (4.6 mm×250 mm, 5 μm) column with mobile phase consisted of acetonitrile- 0.06 mol ·L-1 KDP buffer (38 ∶62) at a flow rate of 1.0 mL ·min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination. Result: The 1inear range of vinorelbine was 2.08～20.8 μg (r=0.999 9).The average recovery was 98.64% (RSD 1.09%). The average entrapment efficiency was 86.83%. Conclusion: This method is simple, precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.关键词：vinorelbine;liposome;content determination;entrapment efficiency
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发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for simultaneous determination of isofraxidin and rosmarinic acid in Qingre Xiaoyanning Capsules. Method: A HPLC method was used with a Waters XBridge C18 column(4.60 mm×250 mm,5 μm) and acetonitrile-0.1% phosphoric acid (21[DK1]∶79) as the mobile phase.The flow rate was 1.0 mL·min-1 and the detection wavelength was 340 nm. Result: The linear ranges of isofraxidin and rosmarinic acid were 0.32-3.2 μg(r=0.999 8) and 0.256 6-2.566 μg (r=0.999 9), respectively. The average recoveries (n =6) were 97.3% (RSD 1.6%) and 98.9% (RSD 1.3%), respectively. Conclusion: This HPLC method is simple, accurate and relilable, and can be used for the quality control of Qingre Xiaoyanning Capsules.关键词：Qingre Xiaoyanning Capsules;Isofraxidin;rosmarinic acid;HPLC;Determinantion
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发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for simultaneous determination of isofraxidin and rosmarinic acid in Qingre Xiaoyanning Capsules.Method:A HPLC method was used with a Waters XBridge C18 column（4.60 mm × 250 mm,5 μm） and acetonitrile-0.1% phosphoric acid（21∶ 79） as the mobile phase.The flow rate was 1.0 mL.min-1 and the detection wavelength was 340 nm.Result:The linear ranges of isofraxidin and rosmarinic acid were 0.32-3.2 μg（r = 0.999 8） and 0.256 6-2.566 μg（r = 0.999 9）,respectively.The average recoveries（n = 6） were 97.3%（RSD 1.6%） and 98.9%（RSD 1.3%）,respectively.Conclusion:This HPLC method is simple,accurate and relilable,and can be used for the quality control of Qingre Xiaoyanning Capsules.关键词：Qingre Xiaoyanning Capsules;Isofraxidin;rosmarinic acid;HPLC;Determinantion
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发布时间：2024-09-23 - 摘要：Objective: The essential oil was extracted from the seed of wild Perilla frutescens , and the constituent was analyzed to provide scientific proof for the search of biological activity compositions from P. frutescens to empolder the natural resource. Method : The essential oil from the seed of wild P. frutescens was extracted by steam distillation, and the components of the essential oil were separated and structurally identified by gas chromatography-mass spectrometry. The essential oil was investigated by anti-oxidation activities and antimicrobial activities. Result : Fifty-six components were identified and accounted for 92.77% of the all peak area. The essential oil had anti-oxidation activity and antimicrobial activity. Conclusion: The main chemical constituents of the essential oil were monoterpenoids and sesquiterpenoids compounds. The major components were perillaketone (10.32%), isoegomaketone (9.73%), caryophyllene(7.96%), α-linalool (6.32%), cinerone (5.28%). The essential oil had obvious anti-oxidation activities for scavenging of ·OH, killed and inhibited the growth of testing bacteria, especially against Staphylococcus aureus CMCC26112 and Candida albicans CMCC850216.关键词：Perilla frutescens;the essential oil;gas chromatography-mass spectrometry;anti-oxidation activity;antimicrobial activity
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发布时间：2024-09-23 - 摘要：Objective:The essential oil was extracted from the seed of wild Perilla frutescens,and the constituent was analyzed to provide scientific proof for the search of biological activity compositions from P.frutescens to empolder the natural resource.Method:The essential oil from the seed of wild P.frutescens was extracted by steam distillation,and the components of the essential oil were separated and structurally identified by gas chromatography-mass spectrometry.The essential oil was investigated by anti-oxidation activities and antimicrobial activities.Result:Fifty-six components were identified and accounted for 92.77% of the all peak area.The essential oil had anti-oxidation activity and antimicrobial activity.Conclusion:The main chemical constituents of the essential oil were monoterpenoids and sesquiterpenoids compounds.The major components were perillaketone（10.32%）,isoegomaketone（9.73%）,caryophyllene（7.96%）,α-linalool（6.32%）,cinerone（5.28%）.The essential oil had obvious anti-oxidation activities for scavenging of.OH,killed and inhibited the growth of testing bacteria,especially against Staphylococcus aureus CMCC26112 and Candida albicans CMCC850216.关键词：Perilla frutescens;the essential oil;gas chromatography-mass spectrometry;anti-oxidation activity;antimicrobial activity
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- 183
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- 10
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for the content determination of euphol in slices of E.kansui and its preparation Kongxian Pills.Method:HPLC-ELSD was carried out on a Dikma Diamonsil C18 column（4.6 mm × 250 mm,5 μm）with methanol-water（99∶ 1） as the mobile phase,the flow rate was 1.0 mL.min-1,and the detector was evaporative light-scattering detector（ELSD）.The column temperature was set at 35 ℃.Result:The calibration curve was linear in the range of 1.04-20.80 μg（r = 0.999 6）,the average recovery was 97.53% with RSD of 2.56%.The content of euphol in ten batches of slices of E.kansui and five batches of Kongxian Pills were determined.Conclusion:The method is simple,accurate and reproducible,and it can be used to determine the content of euphol in slices of E.kansui and Kongxian Pills.关键词：HPLC-ELSD;euphol;slices of Euphorbia kansui;Kongxian Pills;content determination
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- 198
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发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for the content determination of euphol in slices of E. kansui and its preparation Kongxian Pills. Method: HPLC-ELSD was carried out on a Dikma Diamonsil C18 column(4.6 mm×250 mm, 5 μm)with methanol-water (99 ∶1) as the mobile phase, the flow rate was 1.0 mL ·min-1, and the detector was evaporative light-scattering detector(ELSD). The column temperature was set at 35 ℃. Result: The calibration curve was linear in the range of 1.04-20.80 μg ( r=0.999 6), the average recovery was 97.53％with RSD of 2.56％. The content of euphol in ten batches of slices of E. kansui and five batches of Kongxian Pills were determined. Conclusion: The method is simple, accurate and reproducible, and it can be used to determine the content of euphol in slices of E. kansui and Kongxian Pills.关键词：HPLC-ELSD;euphol;slices of Euphorbia kansui;Kongxian Pills;content determination
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发布时间：2024-09-23 - 摘要：Objective: To establish a method to determine quercetin in Heibai Baoganqing Granules of Mao ethnomedicine by HPLC. Method: HPLC was used. The column was Diamonsil (TM) C18 (4.6 mm×250 mm, 5 μm). The mobile phase consisted of methanol-0.2% phosphoric acid (56 ∶44), with the flow rate of 1.0 mL ·min-1 and the wavelength was at 370 nm. Result: Quercetin was linear in the range of 0.020 6～0.133 μg. The average recovery was 98.2%. The average content of quercetin in five samples was 1.44 mg ·g-1. Conclusion: This method is accurate, simple and reproducible, and can be used for the determination of quercetin in Heibai Baoganqing Granules.关键词：Heibai Baoganqing Granules;quercetin;content determination;RP-HPLC
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发布时间：2024-09-23 - 摘要：Objective:To establish a method to determine quercetin in Heibai Baoganqing Granules of Mao ethnomedicine by HPLC.Method:HPLC was used.The column was Diamonsil（TM） C18（4.6 mm × 250 mm,5 μm）.The mobile phase consisted of methanol-0.2% phosphoric acid（56∶ 44）,with the flow rate of 1.0 mL.min-1and the wavelength was at 370 nm.Result:Quercetin was linear in the range of 0.020 6 ～ 0.133 μg.The average recovery was 98.2%.The average content of quercetin in five samples was 1.44 mg.g-1.Conclusion:This method is accurate,simple and reproducible,and can be used for the determination of quercetin in Heibai Baoganqing Granules.关键词：Heibai Baoganqing Granules;quercetin;content determination;RP-HPLC
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- 95
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- 2
发布时间：2024-09-23 - 摘要：Objective:To establish a method to determine the microbial limit test for Jiangdan Kechuan Tablets.Method:According to China Pharmacopoeia edition 2005,the antimicrobial effect of Jiangdan Kechuan Tablets was determined by recovery rate with 5 control trains.Result:Antibacterial effect was found against Staphyloccocus aureus Rosenbach and Bacillus subtilis Cohn.Centrifuging medium and diluting method were used for counting bacteria.Conclusion:The method is simple,feasible and accurate.
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- 47
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发布时间：2024-09-23 - 摘要：Objective: To establish a method to determine the microbial limit test for Jiangdan Kechuan Tablets. Method: According to China Pharmacopoeia edition 2005, the antimicrobial effect of Jiangdan Kechuan Tablets was determined by recovery rate with 5 control trains. Result: Antibacterial effect was found against Staphyloccocus aureus Rosenbach and Bacillus subtilis Cohn. Centrifuging medium and diluting method were used for counting bacteria. Conclusion: The method is simple, feasible and accurate.
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发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for determination of methotrexate concentration in plasma using higher doses.Method:The separation was performed in a Gold column（4.6 mm × 150 mm,5 μm） with a mobile phase of methanol:phosphate buffer（19 ∶ 81,pH 7.2） and determinated at 306 nm.Result:The measurable lowest concentration was 0.002 μmol.L-1.The experiment showed that the recovery from plasma was 90.2%,94.4%,101.2%.The RSD of within day and between day was less than 9.0%.A good linearity was found in the range of 0.01 ～ 1.38 μmol.L-1（r = 0.999 7） and in the range of 1.38 ～ 103.5 μmol.L-1（r = 0.999 5）.Conclusion:The method is simple,sensitive and accurate with wide linear dynamic range.关键词：Methotrexate;HPLC;drug concentration;wide linear dynamic range
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- 262
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发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for determination of methotrexate concentration in plasma using higher doses. Method: The separation was performed in a Gold column(4.6 mm×150 mm,5 μm) with a mobile phase of methanol: phosphate buffer (19∶81,pH 7.2) and determinated at 306 nm.Result: The measurable lowest concentration was 0.002 μmol·L-1. The experiment showed that the recovery from plasma was 90.2%, 94.4%, 101.2%. The RSD of within day and between day was less than 9.0%. A good linearity was found in the range of 0.01～1.38 μmol·L-1 (r=0.999 7) and in the range of 1.38～103.5 μmol·L-1 (r=0.999 5).Conclusion: The method is simple, sensitive and accurate with wide linear dynamic range.关键词：Methotrexate;HPLC;drug concentration;wide linear dynamic range
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发布时间：2024-09-23 - 摘要：Objective:To establish a new determination method for quercitrin from Platycladus orientalis leaves by capillary-zone electrophoresis（CZE）.Method:Under the optimized CZE conditions:a running buffer composed of 30 mmol.L-1 borax-boric acid（volume 1∶ 3）,separation voltage at 25 kV,temperature at 25 centigrade,injection time for 8 sec with 0.5 psi pressure,quercitrin from P.orientalis leaves was detected at 254 nm wavelength.Result:Quercitrin was linear in the range of 1-200 mg.L-1（r = 0.999 3）.The RSD of exactitude,stability,and recovery of the method was 1.1346%,2.6046 % and 2.3315%,respectively.The contents of quercitrin in three batches of P.orientalis leaves were 2.551 3,2.546 7,2.478 0 mg.g-1.Conclusion:The method was simple,fast and accurate for determination of the drugs containing quercitrin.关键词：capillary-zone electrophoresis;Platycladus orientalis leaves;quercitrin
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- 156
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发布时间：2024-09-23 - 摘要：Objective: To establish a new determination method for quercitrin from Platycladus orientalis leaves by capillary-zone electrophoresis (CZE). Method: Under the optimized CZE conditions:a running buffer composed of 30 mmol ·L-1 borax-boric acid (volume 1 ∶3),separation voltage at 25 kV,temperature at 25 centigrade, injection time for 8 sec with 0.5 psi pressure, quercitrin from P. orientalis leaves was detected at 254 nm wavelength. Result: Quercitrin was linear in the range of 1-200 mg ·L-1 (r=0.999 3). The RSD of exactitude, stability, and recovery of the method was 1.1346%, 2.6046 % and 2.3315%, respectively. The contents of quercitrin in three batches of P. orientalis leaves were 2.5513, 2.5467, 2.4780 mg ·g -1. Conclusion: The method was simple, fast and accurate for determination of the drugs containing quercitrin.关键词：capillary-zone electrophoresis;Platycladus orientalis leaves;quercitrin
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发布时间：2024-09-23 - 摘要：Objective: To Analyze the chemical constituents of volatile oil from the leaves and flowers of Dimocarpus longan in Guangxi. Method: The volatile oil was extracted from the leaves and flowers of D. longan . by steam distillation. The constituents of volatile oil were identified by GC/MS technology. Result: The yield of the collected volatile oil of the leaves and flowers of D. longan was 0.35%, 0.20%, repectively. 39 compounds constituting 96.2% of the oil of leaves were identified. 32 compounds constituting 97.01% of the oil of flowers were identified. Conclusion: The GC/MS is a simple, rapid and sensitive method. It can provide a scientific basis for rational use of the leaves and flowers of D. longan in Guangxi.
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发布时间：2024-09-23 - 摘要：Objective:To establish the quality standard of Paozhen Bingdu Tablet.Method:TLC was adopted to identify Rhizoma Coptidis,Fructus Gardeniae,Radix et Rhizoma Cynanchi Paniculati and Pulvis Fellis Suis in Paozhen Bingdu Tablet.HPLC was adopted to determine the content of Baicalin in Paozhen Bingdu Tablet.Result:Rhizoma Coptidis,Fructus Gardeniae,Radix et Rhizoma Cynanchi Paniculati and Pulvis Fellis Suis could be identified by TLC.Baicalin was linear in the range of 0.097 6-2.440 0 μg,r = 0.999 9,the average recovery was 100.91%,RSD 1.24%.Conclusion:The established method is simple,convenient and reoproducible and it can be used for the quality control of Paozhen Bingdu Tablet.关键词：Paozhen Bingdu Tablet;TLC;HPLC;baicalin
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- 78
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- 2
发布时间：2024-09-23 - 摘要：Objective: To establish the quality standard of Paozhen Bingdu Tablet. Method: TLC was adopted to identify Rhizoma Coptidis,Fructus Gardeniae,Radix et Rhizoma Cynanchi Paniculati and Pulvis Fellis Suis in Paozhen Bingdu Tablet. HPLC was adopted to determine the content of Baicalin in Paozhen Bingdu Tablet. Result: Rhizoma Coptidis,Fructus Gardeniae,Radix et Rhizoma Cynanchi Paniculati and Pulvis Fellis Suis could be identified by TLC. Baicalin was linear in the range of 0.0976-2.4400 μg, r=0.999 9, the average recovery was 100.91%, RSD 1.24%. Conclusion: The established method is simple, convenient and reoproducible and it can be used for the quality control of Paozhen Bingdu Tablet.关键词：Paozhen Bingdu Tablet;TLC;HPLC;baicalin
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发布时间：2024-09-23 - 摘要：Objective:To study differences between the essential oil of Thymus mongolicus from Gansu by GC /MS.Method:The essential oils were extracted with steam distillation method.The relative amount of each component was calculated in area normalization.The compounds in oils were isolated by column chromatography to identify the chemical constituents of essential oils as assistant method.Result:Eighty-nine compounds were identified in the three essential oils.The major constituents of oils from Dazonghu region were geraniol,thymol and longifolene,and from Zhayougou were linalool and thymol,ect.The major constituents of oils from Tianshui were sabinene,eucalyptol,thymol,cedrol,ect.Conclusion:The main chemical component of essential oil of T.mongolicus in Gansu were monoterpenes.The differences in growing environment were different with relative content of essential oil composition.关键词：Thymus mongolicus;essential oil;GC /MS;Gansu
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- 295
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发布时间：2024-09-23 - 摘要：Objective: To study differences between the essential oil of Thymus mongolicusfrom Gansu by GC/MS. Method: The essential oils were extracted with steam distillation method.The relative amount of each component was calculated in area normalization. The compounds in oils were isolated by column chromatography to identify the chemical constituents ofessential oils as assistant method. Result: Eighty-nine compounds were identified in the three essential oils. The major constituents of oils from Dazonghu region were geraniol, thymol and longifolene, and from Zhayougou were linalool and thymol, ect. The major constituents of oils from Tianshui were sabinene, eucalyptol, thymol, cedrol, ect. Conclusion: The main chemical component of essential oil of T. mongolicus in Gansu were monoterpenes. The differences in growing environment were different with relative content of essential oil composition.关键词：Thymus mongolicus;essential oil;GC/MS;Gansu
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发布时间：2024-09-23 - 摘要：目的:建立HPLC测定益心舒胶囊中人参皂苷Rg1,Re,Rb1的方法。方法:色谱柱Diamond C18（4.6 mm×250mm,5μm）;流动相乙腈-水梯度洗脱;流速1.0 mL.min-1;检测波长203 nm;柱温为30℃;进样量10μL。结果:人参皂苷Rg1,Re,Rb1分别在2.04～12.38μg,0.295～1.768μg,1.69～10.51μg呈良好的线性关系,三者平均加样回收率分别为96.77%（RSD 1.01%）,96.84%（RSD 0.95%）,97.26%（RSD 0.87%）。结论:本方法灵敏、简便、准确,可用于益心舒胶囊的质量控制。关键词：益心舒胶囊;人参皂苷Rg1;人参皂苷Re;人参皂苷Rb1;含量测定
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- 178
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- 8
发布时间：2024-09-23 - 摘要：Objective:The aim of the paper was to study the relationship between 18 kinds of amino acid contents and cold or hot nature of traditional Chinese material medica and establish discriminant function.Method:Extracting eighteen kinds of amino acid from thirty kinds of TCM.Seventeen kinds of amino acid contents were determined by high performance liquid chromatography（HPLC） with precolumn phenylisothiocyanate derivatization spectrophotometry.Tryptophan were determined by ultraviolet spectrophotometry.The Fisher discriminant analysis was used to analyze the relationship between amino acid and cold or hot nature of traditional Chinese material medica.Result:Form the determinantion of 18 kinds of amino acid contents,it showed that the average amino acid contents of the heat is 1.32 times than the one of the cold,the correct rate is ninety percent analyzed by Fisher discriminant analysis.Conclusion:Form the results of fisher discriminant of 18 variables,it revealed that there are many differences between the cold and hot property in 18 kinds of amino acid contents.Based on the analysis of the determined data,a discrimination function was established and it could distinguish cold and hot property of Chinese herbs.关键词：nature of traditional Chinese material medica;cold or hot property;amino acid;discriminant function
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- 397
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发布时间：2024-09-23 - 摘要：<正>尊敬的作者、读者:由于本刊近来投稿量不断增加,杂志影响力不断扩大,每月一刊的出版周期已无法满足广大科研工作者的投稿及发表需求。经过编辑部研究,主办单位中国中医科学院中药研究所及中国中西医结合学会中药专业委员会、主管单位国家中医药管理局及北京市新闻出版管理局
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发布时间：2024-09-23 - 摘要：Objective: The aim of the paper was to study the relationship between 18 kinds of amino acid contents and cold or hot nature of traditional Chinese material medica and establish discriminant function. Method: Extracting eighteen kinds of amino acid from thirty kinds of TCM. Seventeen kinds of amino acid contents were determined by high performance liquid chromatography(HPLC) with precolumn phenylisothiocyanate derivatization spectrophotometry. Tryptophan were determined by ultraviolet spectrophotometry. The Fisher discriminant analysis was used to analyze the relationship between amino acid and cold or hot nature of traditional Chinese material medica. Result: Form the determinantion of 18 kinds of amino acid contents,it showed that the average amino acid contents of the heat is 1.32 times than the one of the cold,the correct rate is ninety percent analyzed by Fisher discriminant analysis. Conclusion: Form the results of fisher discriminant of 18 variables, it revealed that there are many differences between the cold and hot property in 18 kinds of amino acid contents. Based on the analysis of the determined data,a discrimination function was established and it could distinguish cold and hot property of Chinese herbs.关键词：nature of traditional Chinese material medica;cold or hot property;amino acid;discriminant function
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发布时间：2024-09-23 - 摘要：Objective: To studythe absorptionofsalvianolic acid Banddanshensu. Method: In situ single pass perfusion model was used and the concentration ofsalvianolic acid B and danshensu in perfusate were determined by HPLC in combinationwith ultraviolet detector. Result: The apparent penetration coefficient(Papp) and absorption percentage(Fa)of danshensu andsalvianolic acid B were all low at differentconcentrationin four different regions of intestine of rat (duodenum, jejunum,ileum,colon).The absorption rate of dashensu was faster than salvianolic acid B in this study. The values of the Fa a and the Papp were not significantly affected by concentration of perfusion solution and intestinal segments. Conclusion: The intestine absorption characteristics ofsalvianolic acid B and danhensu was poor at all segments of intestine in rats where there is no special ab sorption site and showed the passive diffusion process.关键词：danshensu;salvianolic acid B;intestine absorption;insitu singlepassperfusionmodel
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发布时间：2024-09-23 - 摘要：Objective:To study the absorption of salvianolic acid B and danshensu.Method:In situ single pass perfusion model was used and the concentration of salvianolic acid B and danshensu in perfusate were determined by HPLC in combination with ultraviolet detector.Result:The apparent penetration coefficient（Papp） and absorption percentage（Fa） of danshensu and salvianolic acid B were all low at different concentration in four different regions of intestine of rat（duodenum,jejunum,ileum,colon）.The absorption rate of dashensu was faster than salvianolic acid B in this study.The values of the Fa a and the Papp were not significantly affected by concentration of perfusion solution and intestinal segments.Conclusion:The intestine absorption characteristics of salvianolic acid B and danhensu was poor at all segments of intestine in rats where there is no special ab sorption site and showed the passive diffusion process.关键词：danshensu;salvianolic acid B;intestine absorption;in situ single pass perfusion model
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- 1225
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of aqueous extract of alcohol of the roots of Apium graveolens on blood pressure and underlying the mechanisms in renal hypertension rats.Method:Two-kidney one clip（2K1C） renal hypertension rats were randomly divided into negative control group,model group,the positive control（Captopril,CAP） group and the roots of A.graveolens extract high,medium low-dose group and,the daily perfusion for 16 week at any time adjust the dose according to weight capacity,periodic determination of rat tail artery systolic and diastolic blood pressure.After the last administration,after separation of the abdominal aorta blood,take heart,left ventricular weight（LVW） / body weight（BW） ratio,superoxide dismutase（SOD） activity,malondialdehyde（MDA）,vascular tone factor Ⅱ（Ang Ⅱ）,endothelin（ET） content.Result:The roots of A.graveolens extract L treatment group was significantly higher SOD activity,lower MDA content and significantly decreased plasma ET levels.The roots of A.graveolens plasma high-dose group were significantly lower Ang Ⅱ levels.Conclusion:The roots of A.graveolens extract can depress blood pressure in rats with renal hypertension.关键词：Uygur medicine;roots of Apium graveolens;renal hypertension
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- 239
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- 8
发布时间：2024-09-23 - 摘要：Objective: To observe the effects of aqueous extract of alcohol of the roots of Apium graveolens on blood pressure and underlying the mechanisms in renal hypertension rats. Method: Two-kidney one clip (2K1C) renal hypertension rats were randomly divided into negative control group, model group, the positive control (Captopril,CAP) group and the roots of A. graveolens extract high, medium low-dose group and, the daily perfusion for 16 week at any time adjust the dose according to weight capacity, periodic determination of rat tail artery systolic and diastolic blood pressure.After the last administration, after separation of the abdominal aorta blood, take heart, left ventricular weight (LVW) / body weight (BW) ratio, superoxide dismutase (SOD) activity, malondialdehyde (MDA), vascular tone factor Ⅱ (Ang Ⅱ), endothelin (ET) content. Result: The roots of A. graveolens extract L treatment group was significantly higher SOD activity, lower MDA content and significantly decreased plasma ET levels. The roots of A. graveolens plasma high-dose group were significantly lower Ang Ⅱ levels. Conclusion: The roots of A. graveolens extract can depress blood pressure in rats with renal hypertension.关键词：Uygur medicine;roots of Apium graveolens;renal hypertension
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发布时间：2024-09-23 - 摘要：Objective: To observe the effect of VEGF-VEGFR pathway on EPCs function induced by Xuefu Zhuyu Tang. Method: After induced by serial concentrations of Xuefu Zhuyu Decoction-contained serum (XZDCS) and blank serum, EPCs proliferation, migration, adhension ability and VEGF, VEGFR expression were detected by MTT, Boyden chamber, adhension test and RT-PCR respectively. Result: Compared to the control group, 10% XZDCS could elevate the cell regeneration and adhension ability. The EPCs migration ability was inhibited by 5% XZDCS and improved by 10%,15% XZDCS. The higher two concentrations could up-regulate VEGF and VEGFR transcription significantly. Conclusion: Xuefu Zhuyu Tang could induce EPCs into angiogenesis by up regulating VEGF-VEGFR pathway to promote its proliferation, migration and adhension function.关键词：Xuefu Zhuyu Tang;EPCs;angiogenesis;VEGF-VEGFR
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of VEGF-VEGFR pathway on EPCs function induced by Xuefu Zhuyu Tang.Method:After induced by serial concentrations of Xuefu Zhuyu Decoction-contained serum（XZDCS） and blank serum,EPCs proliferation,migration,adhension ability and VEGF,VEGFR expression were detected by MTT,Boyden chamber,adhension test and RT-PCR respectively.Result:Compared to the control group,10% XZDCS could elevate the cell regeneration and adhension ability.The EPCs migration ability was inhibited by 5% XZDCS and improved by 10%,15% XZDCS.The higher two concentrations could up-regulate VEGF and VEGFR transcription significantly.Conclusion:Xuefu Zhuyu Tang could induce EPCs into angiogenesis by up regulating VEGF-VEGFR pathway to promote its proliferation,migration and adhension function.关键词：Xuefu Zhuyu Tang;EPCs;angiogenesis;VEGF-VEGFR
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- 535
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- 60
发布时间：2024-09-23 - 摘要：Objective : To expound the mechanism of nourishing kidney and reducing urine by investigating the regulative action for renin-angiotensin-aldosterone system (RAAS) by Suoquan Capsule. Method: The rat model of kidney deficiency and diuresis was established by adenine. The levels of ACE,PRA,AngⅡ and ALD in blood were determined for model group, Suoquan Capsule group,Jinsuo Gujin Wan group and control group with ELISA technology. Result : Compared with model group, Suoquan Capsule could remarkedly increase the levels of ACE,PRA,AngⅡ and ALD in blood of the rats with kidney deficiency and diuresis. Conclusion : Suoquan Capsule can regulate water metabolism by coordinating the function of RAAS.The mechanism of nourishing the kidney and reducing urine by Souquan Capsule appears to be related with endocrinium system.关键词：Suoquan Capsule;deficiency of the kidney with diuresis;renin-angiotensin-aldosterone-system
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发布时间：2024-09-23 - 摘要：Objective:To expound the mechanism of nourishing kidney and reducing urine by investigating the regulative action for renin-angiotensin-aldosterone system（RAAS） by Suoquan Capsule.Method:The rat model of kidney deficiency and diuresis was established by adenine.The levels of ACE,PRA,AngⅡ and ALD in blood were determined for model group,Suoquan Capsule group,Jinsuo Gujin Wan group and control group with ELISA technology.Result:Compared with model group,Suoquan Capsule could remarkedly increase the levels of ACE,PRA,AngⅡ and ALD in blood of the rats with kidney deficiency and diuresis.Conclusion:Suoquan Capsule can regulate water metabolism by coordinating the function of RAAS.The mechanism of nourishing the kidney and reducing urine by Souquan Capsule appears to be related with endocrinium system.关键词：Suoquan Capsule;deficiency of the kidney with diuresis;renin-angiotensin-aldosterone system
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- 345
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- 41
发布时间：2024-09-23 - 摘要：Objective: Proteomics approaches were performed to identify proteins that may be involved in dimethylnitrusamine(DMN)-induced cirrhosis/fibrosis and the effects of Huangqi Tang (HQT) intervention were observed in rats. Method: Differential protein spots that exhibited changes in HQT group in DMN-induced cirrhosis were selected by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization time-of-flight and peptide mass fingerprinting analysis. After that, these protein spots were recognized by bioinformatics information retrieval. Lastly, the results of this proteomic analysis were confirmed by immunoblotting and detection of enzyme activity. Result: Eighteen protein spots were found to be differentially expressed, including 7 spots involved in disorder of lipid metabolism and oxidative damage, 3 spots involved in protein metabolism, 2 spots involved in biotransformation and 3 spots involved in material synthesis and energy metabolism in liver. Among them, 3 spots for confirmation(Prdx6, Hsp70, CAT)were consistent with the results of two-dimensional gel electrophoresis. Conclusion: There are significant oxidative stresses occurred during hepatofibrogenesis of DMN-induced cirrhosis/fibrosis in rats and HQT can significantly improve the antioxidant capacity of liver and protect peroxide damage of cells.关键词：cirrhosis;proteomics;oxidative stress;Huangqi Tang
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发布时间：2024-09-23 - 摘要：Objective:Proteomics approaches were performed to identify proteins that may be involved in dimethylnitrusamine（DMN）-induced cirrhosis /fibrosis and the effects of Huangqi Tang（HQT） intervention were observed in rats.Method:Differential protein spots that exhibited changes in HQT group in DMN-induced cirrhosis were selected by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption /ionization time-of-flight and peptide mass fingerprinting analysis.After that,these protein spots were recognized by bioinformatics information retrieval.Lastly,the results of this proteomic analysis were confirmed by immunoblotting and detection of enzyme activity.Result:Eighteen protein spots were found to be differentially expressed,including 7 spots involved in disorder of lipid metabolism and oxidative damage,3 spots involved in protein metabolism,2spots involved in biotransformation and 3 spots involved in material synthesis and energy metabolism in liver.Among them,3 spots for confirmation（Prdx6,Hsp70,CAT） were consistent with the results of two-dimensional gel electrophoresis.Conclusion:There are significant oxidative stresses occurred during hepatofibrogenesis of DMN-induced cirrhosis/fibrosis in rats and HQT can significantly improve the antioxidant capacity of liver and protect peroxide damage of cells.关键词：cirrhosis;proteomics;oxidative stress;Huangqi Tang
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- 438
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of Liuwei Dihuang Wan on spontaneous breast carcinoma in mice. Method: The spontaneous breast carcinoma derived in kunming mice (clean class) was used as animal model. The mice were given Liuwei Dihuang Wan to their agonal stage. The cancer mass pathology was analyzed by HE staining. The mRNA and protein expressions of vascular endothelial growth factor(VEGF) and cyclin D3 were assayed by RT-PCR and Western blotting. Result: In Liuwei Dihuang Wan group, the tumor weight and expression of VEGF and cyclin D3 gene were obviously lower than that in the control groups(P<0.05). The cell differentiation was also better than that in the control group. Conclusion: Liuwei Dihuang Wan can retrain the growth of cancer and promote the cancer cell differentiation. The possible mechanism is related with the retrained expression of VEGF and cyclin D3 gene.关键词：Liuwei Dihuang Wan;spontaneous breast carcinoma;weight of tumor;vascular endothelial growth factor;cyclin D3;gene expression
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Liuwei Dihuang Wan on spontaneous breast carcinoma in mice.Method:The spontaneous breast carcinoma derived in kunming mice（clean class） was used as animal model.The mice were given Liuwei Dihuang Wan to their agonal stage.The cancer mass pathology was analyzed by HE staining.The mRNA and protein expressions of vascular endothelial growth factor（VEGF） and cyclin D3 were assayed by RT-PCR and Western blotting.Result:In Liuwei Dihuang Wan group,the tumor weight and expression of VEGF and cyclin D3 gene were obviously lower than that in the control groups（P < 0.05）.The cell differentiation was also better than that in the control group.Conclusion:Liuwei Dihuang Wan can retrain the growth of cancer and promote the cancer cell differentiation.The possible mechanism is related with the retrained expression of VEGF and cyclin D3 gene.关键词：Liuwei Dihuang Wan;spontaneous breast carcinoma;weight of tumor;vascular endothelial growth factor;cyclin D3;gene expression
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- 319
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- 18
发布时间：2024-09-23 - 摘要：Objective:To analyze the chemical constitutes and effective components of Fufang Fuling Tang（FFFLT）,a traditional Chinese medicine shown to be effective in treating eczema.Method:A rapid method incorporating of ultra fast liquid chromatography-photodiode array detector-electrospray ionization-mass spectra（UFLC-DAD-ESI-MS） profiling was developed for screening the chemical constitutes in FFFLT.Based on the different peaks from FFFLT samples,four herb groups（Ⅰ-Ⅳ） were established.Herb group I was composed of Poria cocos,Paeonia lactiflora Pall,Gardenia jasminoides,Glycyrrhiza uralensis,Alisma orientalis,Phellodendron amurense,Angelica sinensis and Spirodela polyrrhiza;groupⅡwas composed of P.lactiflora Pall,G.jasminoides,G.uralensis;group Ⅲ was composed of P.cocos and A.sinensis;and group Ⅳ was composed of P.cocos,P.lactiflora Pall,G.jasminoides,G.uralensis and A.sinensis.Sixty mice with allergic contact dermatitis（ACD） induced by DNFB were randomly divided into 6 groups,including Ⅰ-Ⅳ herb treatment groups（HTG）,positive control group（hydrocortisone treatment group,HCTG） and negative control group（physiological saline treatment group,PSTG）.The thickness and weight of mouse ears,the pathological changes in ear tissue and the serum levels of interferon（IFN）-γ,interleukin（IL）-4 and IL-10 were observed.Result:11 chemicals were analyzed and the most abundant constitutes from FFFLT were genipingentiobioside,geniposide,paeoniflorin and liquiritin.Compared with PSTG,significant decrease in ear swelling and dermal inflammatory infiltration were seen inⅠ-Ⅳ HTG and the HCTG（P < 0.05）.ⅠHTG,ⅣHTG and HCTG showed better effects（P < 0.01）.Conclusion:The pharmacodynamics of FFFLT in treating eczema mostly come from Paeonia lactiflora Pall,Gardenia jasminoides,Glycyrrhiza uralensis,Poria cocos and Angelica sinensis.关键词：Fufang Fuling Tang;dermatitis;ultra fast liquid chromatography-photodiode array detector-electrospray ionization-mass spectra
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- 648
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发布时间：2024-09-23 - 摘要：Objective: To analyze the chemical constitutes and effective components of Fufang Fuling Tang (FFFLT), a traditional Chinese medicine shown to be effective in treating eczema. Method: A rapid method incorporating of ultra fast liquid chromatography-photodiode array detector-electrospray ionization-mass spectra (UFLC-DAD-ESI-MS) profiling was developed for screening the chemical constitutes in FFFLT. Based on the different peaks from FFFLT samples, four herb groups(Ⅰ-Ⅳ) were established. Herb group I was composed of Poria cocos, Paeonia lactiflora Pall, Gardenia jasminoides, Glycyrrhiza uralensis, Alisma orientalis, Phellodendron amurense, Angelica sinensis and Spirodela polyrrhiza; group Ⅱ was composed of P. lactiflora Pall, G. jasminoides, G. uralensis; group Ⅲ was composed of P. cocos and A. sinensis; and group Ⅳ was composed of P. cocos, P. lactiflora Pall, G. jasminoides, G. uralensis and A. sinensis. Sixty mice with allergic contact dermatitis (ACD) induced by DNFB were randomly divided into 6 groups, including Ⅰ-Ⅳ herb treatment groups(HTG), positive control group (hydrocortisone treatment group, HCTG) and negative control group (physiological saline treatment group, PSTG). The thickness and weight of mouse ears, the pathological changes in ear tissue and the serum levels of interferon (IFN)-γ, interleukin (IL)-4 and IL-10 were observed. Result: 11 chemicals were analyzed and the most abundant constitutes from FFFLT were genipingentiobioside,geniposide, paeoniflorin and liquiritin. Compared with PSTG, significant decrease in ear swelling and dermal inflammatory infiltration were seen in Ⅰ-Ⅳ HTG and the HCTG (P<0.05).ⅠHTG,ⅣHTG and HCTG showed better effects (P<0.01). Conclusion: The pharmacodynamics of FFFLT in treating eczema mostly come from Paeonia lactiflora Pall, Gardenia jasminoides, Glycyrrhiza uralensis, Poria cocos and Angelica sinensis.关键词：Fufang Fuling Tang;dermatitis;ultra fast liquid chromatography-photodiode array detector-electrospray ionization-mass spectra
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发布时间：2024-09-23 - 摘要：目的:研究姜黄活性成分对血小板聚集及小鼠常压耐缺氧能力的影响。方法:①采用比浊法观察姜黄活性成分对二磷酸腺苷（ADP）,肾上腺素（Adr）诱发的家兔血小板聚集的影响。②采用常压耐缺氧实验观察姜黄活性成分对小鼠常压耐缺氧能力的影响。结果:①川郁金油（CYJY）0.01,0.001 g.L-1对Adr诱导的血小板聚集有明显的抑制作用。②CYJY,CYJY 0.001 g.L-1对ADP诱导的血小板聚集有较明显的抑制作用。③与空白组比较,姜黄提取物J1,J2,J3,EZY能显著延长小鼠的存活时间。结论:①CYJY 0.01,0.001 g.L-1对ADP及Adr所诱导的血小板聚集均有明显的抑制作用,并显示剂量依赖性。②姜黄提取物姜黄素Ⅰ,Ⅱ,Ⅲ（J1,J2,J3）莪术油（EZY）具有显著提高常压耐缺氧能力。关键词：姜黄;血小板聚集;常压耐缺氧能力
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- 336
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- 16
发布时间：2024-09-23 - 摘要：Objective: To understand the correspondence between syndrome of hyperactivity of liver-yang and the prescriptions of Lingjiao Jiangya Prescription (LJP) and Tianma Gouteng Prescription (TMGTP). Method: Aconium carmichaeli Debx., ephedrine and salty water were used to establish the rat model of hypertension with syndrome of hyperactivity of liver-yang. Fifty SD rats were divided into five groups, including normal control, model, LJP, TMGTP and Jinkuishenqi Prescription (JKSQP). The medicines were given for 28 days, facial temperature, grip strength, systolic pressure, diastolic pressure and mean arterial pressure were determined per 7 days, and rat temper and coat color were observed per 14 days. Result: On day14, LJP and TMGTP could decrease the facial temperature and the blood pressure. On day21, all three prescriptions could strengthen rat grip strength. On day28, the prescriptions could ameliorate rat coat color, and LJP and TMGTP could keep rats calm. Conclusion: LJP and TMGTP could restrain hyperactivity of liver-yang, ameliorate yin-deficiency, relieve hypertension, and decrease the systolic pressure. So the correspondence between the prescriptions of LJP and TMGTP and hypertension with syndrome of hyperactivity of liver-yang has been confirmed.关键词：hyperactivity of liver-Yang;hypertension;correspondence
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发布时间：2024-09-23 - 摘要：Objective:To understand the correspondence between syndrome of hyperactivity of liver-yang and the prescriptions of Lingjiao Jiangya Prescription（LJP） and Tianma Gouteng Prescription（TMGTP）.Method:Aconium carmichaeli Debx.,ephedrine and salty water were used to establish the rat model of hypertension with syn-drome of hyperactivity of liver-yang.Fifty SD rats were divided into five groups,including normal control,model,LJP,TMGTP and Jinkuishenqi Prescription（JKSQP）.The medicines were given for 28 days,facial temperature,grip strength,systolic pressure,diastolic pressure and mean arterial pressure were determined per 7 days,and rat temper and coat color were observed per 14 days.Result:On day14,LJP and TMGTP could decrease the facial temperature and the blood pressure.On day21,all three prescriptions could strengthen rat grip strength.On day28,the prescriptions could ameliorate rat coat color,and LJP and TMGTP could keep rats calm.Conclusion:LJP and TMGTP could restrain hyperactivity of liver-yang,ameliorate yin-deficiency,relieve hypertension,and decrease the systolic pressure.So the correspondence between the prescriptions of LJP and TMGTP and hypertension with syn-drome of hyperactivity of liver-yang has been confirmed.关键词：hyperactivity of liver-Yang;hypertension;correspondence
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- 451
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- 28
发布时间：2024-09-23 - 摘要：Objective: To study the inhibitory effect of Boschniakia rossica extracts (BRE) on lipid peroxidation of plasma lipoproteins in rabbits with hyperlipidemia. Method: The hyperlipidemic model were established by feeding rabbits with high-fat diet. After 8 weeks of treatment, plasma lipoproteins were isolated, and then lipid peroxide (LPO) and susceptibility of low density lipoprotein (LDL) to oxidation was investigated. Result: After the administration with BRE, the levels of plasma total cholesterol (TC), very low density lipoprotein cholesterol (VLDL-CH) and low density lipoprotein cholesterol (LDL-CH) in hyperlipidemic rabbits were significantly reduced; LPO levels of plasma and lipoproteins were significantly decreased; and the lag phage of LDL oxidation curve in vitro was noticeably prolonged. Conclusion: BRE, which can reduce blood lipids, decrease the lipid peroxidizability of lipoproteins and promote resistance to LDL oxidation, may be useful in the prevention and treatment of atherosclerosis.关键词：Boschniakia rossica;hyperlipidemia;blood lipid;lipoproteins;lipid hydroperoxide;oxidative susceptibility
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发布时间：2024-09-23 - 摘要：Objective:To study the inhibitory effect of Boschniakia rossica extracts（BRE） on lipid peroxi-dation of plasma lipoproteins in rabbits with hyperlipidemia.Method:The hyperlipidemic model were established by feeding rabbits with high-fat diet.After 8 weeks of treatment,plasma lipoproteins were isolated,and then lipid peroxide（LPO） and susceptibility of low density lipoprotein（LDL） to oxidation was investigated.Result:After the administration with BRE,the levels of plasma total cholesterol（TC）,very low density lipoprotein cholesterol（VLDL-CH） and low density lipoprotein cholesterol（LDL-CH） in hyperlipidemic rabbits were significantly re-duced;LPO levels of plasma and lipoproteins were significantly decreased;and the lag phage of LDL oxidation curve in vitro was noticeably prolonged.Conclusion:BRE,which can reduce blood lipids,decrease the lipid per-oxidizability of lipoproteins and promote resistance to LDL oxidation,may be useful in the prevention and treatment of atherosclerosis.关键词：Boschniakia rossica;hyperlipidemia;blood lipid;lipoproteins;lipid hydroperoxide;oxidative susceptibility
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- 108
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发布时间：2024-09-23 - 摘要：Objective:To explore the protective mechanism of Xinjia Xiayuxue Tang on kidneys in diabetic rats by investigating the effect of Xinjia Xiayuxue Tang on bone morphogenetic protein 7（BMP7） and transforming growth factor β1（TGFβ1）in kidneys of diabetic rats.Method:SD rats were divided into control group and diabetes model group.The diabetes model was established by injection of alloxan through vena caudalis,and then were randomly divided into diabetes model（DM） group and Xinjia Xiayuxue Tang-treated group.After 12 weeks,The fast-ing blood glucose（FBG）,serum creatinine（Scr）,urine protein（Upro） were checked with biochemical methods.The morphological changes of renal tissue were examined under microscopy on sections stained with PAS.The mRNA expression of BMP7,TGFβ1 and plasminogen activator inhibitor 1（PAI1）were tested by RT-PCR.Result:Compared with DM model group,in Xinjia Xiayuxue Tang-treated group,FBG,Scr and Upro were markedly decreased（P < 0.01）;renal damage were significantly reduced,the expression of TGFβ1 and PAI1 mRNA were significantly reduced,BMP7 mRNA expression was greatly increased.Conclusion:Xinjia Xiayuxue Tang exerted protective effect on diabet-ic nephropathy probably via elevation of BMP7 and reduction of TGFβ1,then reducing the expression of PAI1.关键词：Xinjia Xiayuxue Tang;diabetic nephropathy;bone morphogenetic protein7;transforming growth factor β1
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- 176
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发布时间：2024-09-23 - 摘要：Objective: To explore the protective mechanism of Xinjia Xiayuxue Tang on kidneys in diabetic rats by investigating the effect of Xinjia Xiayuxue Tang on bone morphogenetic protein 7(BMP7) and transforming growth factor β1(TGFβ1 )in kidneys of diabetic rats. Method: SD rats were divided into control group and diabetes model group. The diabetes model was established by injection of alloxan through vena caudalis,and then were randomly divided into diabetes model(DM) group and Xinjia Xiayuxue Tang -treated group. After 12 weeks,The fasting blood glucose (FBG), serum creatinine (Scr),urine protein (Upro) were checked with biochemical methods. The morphological changes of renal tissue were examined under microscopy on sections stained with PAS. The mRNA expression of BMP7, TGFβ1 and plasminogen activator inhibitor 1(PAI1)were tested by RT-PCR. Result: Compared with DM model group, in Xinjia Xiayuxue Tang -treated group, FBG, Scr and Upro were markedly decreased (P<0.01);renal damage were significantly reduced,the expression of TGFβ1 and PAI1 mRNA were significantly reduced, BMP7 mRNA expression was greatly increased. Conclusion: Xinjia Xiayuxue Tang exerted protective effect on diabetic nephropathy probably via elevation of BMP7 and reduction of TGFβ1, then reducing the expression of PAI1.关键词：Xinjia Xiayuxue Tang;diabetic nephropathy;bone morphogenetic protein7;transforming growth factor β1
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发布时间：2024-09-23 - 摘要：Objective:To investigate effects of Wutian Baogan Ye on alcohol hepatic fibrosis in rats.Meth-od:One hundred Wistar rats were divided randomly into six groups:a normal group（10）,a model control group（18）,Wutian Baogan Ye low dose group（18）,middle dose group（18）,high dose group（18）,and Yishanfu group（18）.Rat model of alcohol hepatic fibrosis was induced by perfusing stomach with the mixture of alcohol,pyrazol and corn oil.After 12 weeks intervention,the indexes of hepatic fiborsis and expression of α-SMA,PPARγ mRNA in liver tissue were investigated for rats in different groups.Result:Compared with model rats,Wutian Ban-gan Ye（especially the middle and the high dose） and Yishanfu could suppress the development of fibrosis,the lev-els of PⅢP,LN in serum and the expression of α-SMA,and increase the expression of PPAR-γ mRNA.The ex-pression of α-SMA and PPARγ was negatively correlated.Conclusion:Wutian Baogan Ye has an active effect on inhibition of alcohol hepatic fibrosis in rats,with a possible mechanism of promoting the expression of PPARγ.关键词：Wutian Baogan Ye;alcohol hepatic fibrosis;Peroxisome proliferator-activated receptor γ
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- 131
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发布时间：2024-09-23 - 摘要：Objective: To investigate effects of Wutian Baogan Ye on alcohol hepatic fibrosis in rats. Method: One hundred Wistar rats were divided randomly into six groups: a normal group (10),a model control group(18), Wutian Baogan Ye low dose group(18), middle dose group(18), high dose group(18), and Yishanfu group(18). Rat model of alcohol hepatic fibrosis was induced by perfusing stomach with the mixture of alcohol, pyrazol and corn oil. After 12 weeks intervention, the indexes of hepatic fiborsis and expression of α-SMA, PPARγ mRNA in liver tissue were investigated for rats in different groups. Result: Compared with model rats, Wutian Bangan Ye (especially the middle and the high dose) and Yishanfu could suppress the development of fibrosis, the levels of PⅢP, LN in serum and the expression of α-SMA, and increase the expression of PPAR-γ mRNA. The expression of α-SMA and PPARγ was negatively correlated. Conclusion: Wutian Baogan Ye has an active effect on inhibition of alcohol hepatic fibrosis in rats, with a possible mechanism of promoting the expression of PPARγ.关键词：Wutian Baogan Ye;alcohol hepatic fibrosis;Peroxisome proliferator-activated receptor γ
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发布时间：2024-09-23 - 摘要：目的:观察桂郁金醇提物对小鼠出血时间和凝血时间的影响。方法:小鼠ig桂郁金醇提物（8,16,32 g.kg-1）1次或1日1次连续5 d,采用断尾法测定出血时间、毛细管法和玻片法测定凝血时间。结果:1日1次连续给药5 d,3个剂量组的桂郁金醇提物对小鼠的凝血时间均无明显影响;1次给药或1日1次连续给药5 d,3个剂量组的桂郁金醇提物均能缩短小鼠的出血时间。结论:桂郁金醇提物具有一定程度的止血作用,但凝血作用不明显。关键词：桂郁金;醇提物;凝血时间;出血时间
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- 366
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发布时间：2024-09-23 - 摘要：目的:研究评价中药外用对骨癌疼痛模型的止痛和骨保护作用。方法:大鼠左后肢胫骨接种转移性大鼠乳腺癌细胞株MRMT-1细胞以制成骨癌疼痛模型按体重将动物分为模型组、唑来膦酸组和中药组;同时设假手术组。中药组于造模后2～21 d用3%化瘀止痛方浸剂浸泡后肢,60 min/次,Bid;以唑来膦酸为阳性对照30μg.kg-1于造模后第5,7,9,12,14,16,19天ip,造模后第19天检测痛行为双足负重差值,第21天测定胫骨标本骨密度。结果:与模型组比较,中药组和唑来膦酸组的双足负重差值降低,胫骨骨质破坏明显改善,骨密度增高。结论:外用中药化瘀止痛方可有效治疗骨癌疼痛,减轻骨转移所致的溶骨性骨质破坏。关键词：骨转移癌疼痛;中药;化瘀止痛;骨密度
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- 176
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发布时间：2024-09-23 - 摘要：目的:观察复方牛蒡子含片的抗炎作用及其对急性咽炎模型大鼠的治疗作用。方法:采用小鼠耳廓肿胀实验及大鼠羧甲基纤维素囊袋实验,观察复方牛蒡子含片的抗炎作用;通过制备急性咽炎模型大鼠,观察复方牛蒡子含片对急性咽炎模型大鼠的治疗作用。结果:复方牛蒡子含片可抑制小鼠耳廓炎性肿胀程度及炎性灶内白细胞的聚集,可减轻急性咽炎模型大鼠咽腔组织黏膜的炎症表现,使急性咽炎症状缓解,且随药物剂量的增加其抗炎作用和对咽炎黏膜的改善作用均增强。结论:复方牛蒡子含片良好的抗炎作用是其治疗急性咽炎的一个重要药理学作用基础。关键词：复方牛蒡子;含片;抗炎;急性咽炎模型
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- 774
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发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of Sulfur fumigated Dioscorea opposita on antioxidation,Ca2+Mg2+-ATPase,Na+K+-ATPase in liver of rats. Method: Wistar rats were divided randomLy into 4 groups, one control and three experimental groups administrated diets mixed with concentrated extractions from unfumigated, commercial and sulfur fumigated D. opposita respectively. The activities of Ca2+Mg2+-ATPase and Na+K+-ATPase and the level of MDA and activities of SOD and GSH-Px 14 weeks after treatment were measured and observe liver framework of organization change. Result: Results showed that activities of GSH-Px 14 weeks after treatment of unfumigated, commercial D. opposita were significantly increased compared with control group; commercial D. opposita enhanced the activities of Na+K+-ATPase. Conclusion: The long-term use of D. opposita has the possibility to cause lipid peroxidation and change the activities of Na+K+-ATPase in liver.关键词：Dioscores opposita;sulphur fumigation;liver;lipid peroxidation;ATPase
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发布时间：2024-09-23 - 摘要：Objective:To investigate the influence of Sulfur fumigated Dioscorea opposita on antioxidation,Ca2 + Mg2 +-ATPase,Na + K +-ATPase in liver of rats.Method:Wistar rats were divided randomLy into 4 groups,one control and three experimental groups administrated diets mixed with concentrated extractions from unfumigated,commercial and sulfur fumigated D.opposita respectively.The activities of Ca2 + Mg2 +-ATPase and Na + K +-ATPase and the level of MDA and activities of SOD and GSH-Px 14 weeks after treatment were measured and observe liver framework of organization change.Result:Results showed that activities of GSH-Px 14 weeks after treatment of un-fumigated,commercial D.opposita were significantly increased compared with control group;commercial D.opposi-ta enhanced the activities of Na + K +-ATPase.Conclusion:The long-term use of D.opposita has the possibility to cause lipid peroxidation and change the activities of Na + K +-ATPase in liver.关键词：Dioscores opposita;sulphur fumigation;liver;lipid peroxidation;ATPase
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- 327
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- 41
发布时间：2024-09-23 - 摘要：Objective:To observe the actute toxicity of volatile oil from Bupleurum chinense in mice and rats and evaluate its safety.Method:Ig volatile oil from B.chinense of different dosages to mice and rats;the acute toxi-cal symptoms,signs,death time and accumulated death rate were observed and the LD50 was calculated by Bliss method.Result:Restlessness,abnormal walking,accelerated heart rate,breathlessness,consecutive convulsion were the main acute toxical symptoms and signs caused by volatile oil from B.chinense;the LD50 and its 95% confident limit of volatile oil of B.chinense to rats and mice are 2.081（1.759 9-2.461 3） mL.kg-1 and 3.118（2.872 4-3.391 2）mL.kg-1 differently.Conclusion:Ig volatile oil from B.chinense to rats and mice can cause acute toxi-cal injury to them.The further study on toxical components,toxical cheminisms and toxical targets of volatile oil from B.chinense are need to do,so as to offer experimental basement for the safe and effective application of B.chinense and its preparation.关键词：volatile oil from Bupleurum chinense;acute toxicity;half lethal dosage
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- 752
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发布时间：2024-09-23 - 摘要：Objective: To observe the actute toxicity of volatile oil from Bupleurum chinense in mice and rats and evaluate its safety. Method: Ig volatile oil from B. chinense of different dosages to mice and rats; the acute toxical symptoms, signs,death time and accumulated death rate were observed and the LD50 was calculated by Bliss method. Result: Restlessness,abnormal walking,accelerated heart rate,breathlessness,consecutive convulsion were the main acute toxical symptoms and signs caused by volatile oil from B. chinense ;the LD50 and its 95% confident limit of volatile oil of B. chinense to rats and mice are 2.081(1.759 9-2.461 3)mL·kg-1 and 3.118(2.872 4-3.391 2)mL·kg-1 differently. Conclusion: Ig volatile oil from B. chinense to rats and mice can cause acute toxical injury to them.The further study on toxical components,toxical cheminisms and toxical targets of volatile oil from B. chinense are need to do,so as to offer experimental basement for the safe and effective application of B. chinense and its preparation.关键词：volatile oil from Bupleurum chinense;acute toxicity;half lethal dosage
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发布时间：2024-09-23 - 摘要：Objective: To study the effects of Modified Xionggui Decoction(MXD) on protection from cerebral ischemia injury and its influence on hemorheological parameters in rats. Method: Seventy of Wistar rats were undergone right middle cerebral artery occlusion (MCAO) according the method of intraluminal thread,then were randomly divided into 5 groups. Four groups were administered MXD (0.38,0.76,1.52 g ·kg-1) and Tongmai Keli (2.7 g ·kg-1) respectively. MCAO model group and sham-operation control group of fifteen rats were administered with the same volume of water. The neurologic findings were scored,hemorheological parameters were measured,and the size of cerebral infarction was determined by TTC-stained method,and histopathologic changes were observed. Result: MXD and Tongmai Keli significantly improved neurologic function after administered drugs for 5 days. MXD and Tongmai Keli significantly reduced blood viscosity of high shear rate, reduced blood viscosity and plasma viscosity,and significantly lessened the size of cerebral infarction and lightened histopathologic changes after administered for 7 days. Conclusion: MXD may improve the obstruction of neurologic function,and protect brain from ischemia injury. The effect of MXD might be related with improvement in hemorheological parameters.关键词：Xionggui Decoction;cerebral ischemia;hemorheology;obstruction of neurologic function
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of Modified Xionggui Decoction（MXD） on protection from cere-bral ischemia injury and its influence on hemorheological parameters in rats.Method:Seventy of Wistar rats were undergone right middle cerebral artery occlusion（MCAO） according the method of intraluminal thread,then were randomly divided into 5 groups.Four groups were administered MXD（0.38,0.76,1.52 g.kg-1） and Tongmai Keli（2.7 g.kg-1） respectively.MCAO model group and sham-operation control group of fifteen rats were administered with the same volume of water.The neurologic findings were scored,hemorheological parameters were measured,and the size of cerebral infarction was determined by TTC-stained method,and histopathologic changes were observed.Result:MXD and Tongmai Keli significantly improved neurologic function after administered drugs for 5 days.MXD and Tongmai Keli significantly reduced blood viscosity of high shear rate,reduced blood viscosity and plasma viscos-ity,and significantly lessened the size of cerebral infarction and lightened histopathologic changes after administered for 7 days.Conclusion:MXD may improve the obstruction of neurologic function,and protect brain from ischemia injury.The effect of MXD might be related with improvement in hemorheological parameters.关键词：Xionggui Decoction;cerebral ischemia;hemorheology;obstruction of neurologic function
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发布时间：2024-09-23 - 摘要：Objective:To study the immune protective mechanism of Qingying Jiebiao Heji on A/PR/8 / H1N1 virus infected mice.Method:The influenza model in mice was established through dropping A /PR /8 /H1N1 virus into the nose of Kunming mice.Ribavirin and Qingying Jiebiao Heji were given to the infected mice respective-ly,then death protection and life prolongation were determined;thymus index and spleen index were observed;Th1（IFN-γ） /Th2（IL-10） expression in peripheral blood was measured by flow cytometry.Result:After being infec-ted,the death protection rate and life prolongation rate of Qingying Jiebiao Heji were 52.94% and 37.64% accord-ingly,the thymus index and spleen index were improved apparently.Th1cytokine IFN-γ attenuated and Th2cytokine IL-10 dominantly expressed.The expression of Th1（IFN-γ） /Th2（IL-10） was remarkably depressed and adjusted,Th2 expression of Qingying Jiebiao Heji group was markedly strengthened and Th1 / Th2 drifted towards the normal level.Conclusion:Qingying Jiebiao Heji has the effect of death protection on the A /PR /8 /H1N1 virus infected mice,it also can reverse its peripheral blood cytokine Th1drifting to Th2,and prevent influenza through immune reg-ulation.关键词：influenza;Qingying Jiebiao Heji;Th1 /Th2;IFN-γ;IL-10
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- 150
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发布时间：2024-09-23 - 摘要：Objective: To study the immune protective mechanism of Qingying Jiebiao Heji on A/PR/8/H1N1 virus infected mice. Method: The influenza model in mice was established through dropping A/PR/8/H1N1 virus into the nose of Kunming mice. Ribavirin and Qingying Jiebiao Heji were given to the infected mice respectively, then death protection and life prolongation were determined; thymus index and spleen index were observed; Th1(IFN-γ)/Th2(IL-10) expression in peripheral blood was measured by flow cytometry. Result: After being infected, the death protection rate and life prolongation rate of Qingying Jiebiao Heji were 52.94% and 37.64% accordingly, the thymus index and spleen index were improved apparently. Th1 cytokine IFN-γ attenuated and Th2 cytokine IL-10 dominantly expressed. The expression of Th1 (IFN-γ)/Th2 (IL-10) was remarkably depressed and adjusted, Th2 expression of Qingying Jiebiao Heji group was markedly strengthened and Th1 / Th2 drifted towards the normal level. Conclusion: Qingying Jiebiao Heji has the effect of death protection on the A/PR/8/H1N1 virus infected mice, it also can reverse its peripheral blood cytokine Th1 drifting to Th2, and prevent influenza through immune regulation.关键词：influenza;Qingying Jiebiao Heji;Th1/Th2;IFN-γ;IL-10
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Pressure-sensitive Adhesives Shujin Paste（SJP） on activa-ting blood circulation and anti-inflammation.Method:The effects of SJP on blood viscosity and platelet aggregation of rats were observed.anti-inflammatory action was investigated through experimental granuloma in rats,the animal model was induced by injection of fresh egg white to the rats,model of ear swelling of mice was also used.Result:The SJP could reduce obviously the blood viscosity of the rabbit,could inhibit the weight gaining of granuloma in rats,and paw swelling induced by fresh egg white in rats,ear inflammation induced by xylene in mice.Conclu-sions:The SJP could promote blood circulation to remove blood stasis and anti-inflammation.
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发布时间：2024-09-23 - 摘要：Objective: To observe the effects of Pressure-sensitive Adhesives Shujin Paste(SJP) on activating blood circulation and anti-inflammation. Method: The effects of SJP on blood viscosity and platelet aggregation of rats were observed. anti-inflammatory action was investigated through experimental granuloma in rats, the animal model was induced by injection of fresh egg white to the rats, model of ear swelling of mice was also used. Result: The SJP could reduce obviously the blood viscosity of the rabbit, could inhibit the weight gaining of granuloma in rats, and paw swelling induced by fresh egg white in rats, ear inflammation induced by xylene in mice. Conclusions: The SJP could promote blood circulation to remove blood stasis and anti-inflammation.
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Shenqi Bufei Tang on collagen deposition and fibronectin（FN） of COPD rat model with lung-qi deficiency syndrome.Method:COPD rat model with lung-qI deficiency syn-drome was established by quantitative stimulation with tobacco,SO2 and papain.Sixty male rats were randomly divid-ed into 6 groups:normal group,model group,low dose treatment group,medium dose treatment group,high dose treatment group,and glucocorticoid treatment group.Collagen deposition in Masson stained slides was observed un-der light microscope and expression of collagen was determined by computer image analyzer.The contents of FN in the BALF were tested by radioimmunoassay.Result:The expressions of collagen in small brouch and lung tissue and the contents of FN in the BALF of the model group were significanthly higher than those of the normol group（P < 0.01）.After treatment,the expressions of collagen and the contents of FN in the BALF in the high and middle dose group were remarkably decreased than those in the modle group（P < 0.01）.Conclusion:Shenqi Bufei Tang may inhibit the deposition of collagen and fibronectin,influence airway remodeling of COPD rat model with lung-qi deficiency syndrome.
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发布时间：2024-09-23 - 摘要：Objective: To observe the effects of Shenqi Bufei Tang on collagen deposition and fibronectin (FN) of COPD rat model with lung-qi deficiency syndrome. Method: COPD rat model with lung-qI deficiency syndrome was established by quantitative stimulation with tobacco,SO2 and papain.Sixty male rats were randomly divided into 6 groups: normal group, model group, low dose treatment group, medium dose treatment group, high dose treatment group, and glucocorticoid treatment group. Collagen deposition in Masson stained slides was observed under light microscope and expression of collagen was determined by computer image analyzer. The contents of FN in the BALF were tested by radioimmunoassay. Result: The expressions of collagen in small brouch and lung tissue and the contents of FN in the BALF of the model group were significanthly higher than those of the normol group(P<0.01). After treatment,the expressions of collagen and the contents of FN in the BALF in the high and middle dose group were remarkably decreased than those in the modle group (P<0.01). Conclusion: Shenqi Bufei Tang may inhibit the deposition of collagen and fibronectin, influence airway remodeling of COPD rat model with lung-qi deficiency syndrome.
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Supplemented Siwu Decoction（SSWD） on blood pressure and left ventricular hypertrophy of spontaneous hypertension rats（SHR）.Method:Sixty SHR were randomly divided into six groups（n = 10,each）:SSWD high dose group（31.52 g.kg-1.d-1）,SSWD middle dose group（15.74 g.kg-1.d-1）,SSWD low dose group（7.81 g.kg-1.d-1）,model group（with normal saline）,Niuhuang Jiangya Wan group（0.17 g.kg-1.d-1） and Captopril group（7.88 g.kg-1.d-1）.SBP and left ventricular mass index（LVMI）,the content of left ventricular myocardical interstitial collagen and the collagen ratio of typeⅠto type Ⅲ at each groups were measured.Result:SSWD of high dose reduced SBP significantly compared with the model group（P < 0.01）,the antihypertensive effect was remaining and lasting during whole investigation.LVMI of high dose group reduced remarkly compared to that in the model group（P < 0.05）,the content of left ventricular myocardical collagen was lower than that in the model group,the deposition of left ventricular myocardical interstitial collagen of high dose group decreased pronouncedly compared to the model group.The outcomes from the microscope and elec-tronic microscope indicated that the damages in SSWD group were much slighter than those of the model group.Conclusion:SSWD could effectively lower blood pressure and led to regression of left ventricular hypertrophy in SHR.关键词：Supplemented Siwu Decoction;spontaneous hypertension rat;blood pressure;left ventricular hypertrophy
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发布时间：2024-09-23 - 摘要：Objective: To observe the effect of Supplemented Siwu Decoction(SSWD) on blood pressure and left ventricular hypertrophy of spontaneous hypertension rats(SHR). Method : Sixty SHR were randomly divided into six groups(n=10, each):SSWD high dose group(31.52 g ·kg-1·d-1), SSWD middle dose group(15.74 g ·kg-1·d-1), SSWD low dose group(7.81 g ·kg-1·d-1), model group(with normal saline), Niuhuang Jiangya Wan group(0.17 g ·kg-1·d-1) and Captopril group(7.88 g ·kg-1·d-1). SBP and left ventricular mass index(LVMI),the content of left ventricular myocardical interstitial collagen and the collagen ratio of typeⅠto type Ⅲ at each groups were measured. Result : SSWD of high dose reduced SBP significantly compared with the model group (P<0.01), the antihypertensive effect was remaining and lasting during whole investigation. LVMI of high dose group reduced remarkly compared to that in the model group (P<0.05), the content of left ventricular myocardical collagen was lower than that in the model group, the deposition of left ventricular myocardical interstitial collagen of high dose group decreased pronouncedly compared to the model group.The outcomes from the microscope and electronic microscope indicated that the damages in SSWD group were much slighter than those of the model group. Conclusion: SSWD could effectively lower blood pressure and led to regression of left ventricular hypertrophy in SHR.关键词：Supplemented Siwu Decoction;spontaneous hypertension rat;blood pressure;left ventricular hypertrophy
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发布时间：2024-09-23 - 摘要：目的:测定党参中二氧化硫（SO2）含量,比较含硫量不同的党参对小鼠的抗应激作用及对免疫器官质量影响的差异。方法:用水蒸气蒸馏法测定3种受熏度不同党参的含硫量;比较不同含硫量党参对小鼠的游泳时间、耐缺氧时间、气管结扎存活时间和免疫器官指数。结果:脱硫党参比含硫党参具有延长小鼠耐缺氧、气管结扎存活时间及增加脾重的作用,对小鼠的游泳时间,无统计学意义。结论:脱硫党参与含硫量党参比较,前者具有显著延长小鼠的耐缺氧时间、结扎气管存活时间和增加脾重的作用。关键词：党参;抗应激;免疫器官;药效比较
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发布时间：2024-09-23 - 摘要：Objective:To study the antitumor mechanism of petroleum ether extract of pine needles.Meth-od:Antitumor activity of the petroleum ether extract was determined by means of transplantable tumor（S 180） in mice.Immunohistochemical methods were employed for detection of Bax,Bcl-2 in tumor tissues.Result:The inhi-bition rate of peftroleum ether extract2.0 g.（kg.d）-1 on S 180-bearing mice was 42.16%.As for 1.0 g.（kg.d）-1 and 0.5 g.（kg.d）-1 groups,the inhibition rates were 41.71% and 31.16%.Expression of Bax in tumors was obviously increased and Bcl-2 was obviously decreased in all the therapy groups compared with the tumor control group（P < 0.01）.Conclusion:Petroleum ether extract from pinee needles possesses definite antitumor effect.Ap-optosis is one of its antitumor mechanisms.关键词：pine needles;antitumor;mechanism;apoptosis
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发布时间：2024-09-23 - 摘要：Objective: To study the antitumor mechanism of petroleum ether extract of pine needles. Method: Antitumor activity of the petroleum ether extract was determined by means of transplantable tumor (S180) in mice. Immunohistochemical methods were employed for detection of Bax, Bcl-2 in tumor tissues. Result: The inhibition rate of peftroleum ether extract2.0 g·(kg·d) -1 on S180-bearing mice was 42.16%. As for 1.0 g·(kg·d) -1 and 0.5 g·(kg·d) -1 groups, the inhibition rates were 41.71% and 31.16%. Expression of Bax in tumors was obviously increased and Bcl-2 was obviously decreased in all the therapy groups compared with the tumor control group (P<0.01). Conclusion: Petroleum ether extract from pinee needles possesses definite antitumor effect.关键词：pine needles;antitumor;mechanism;apoptosis
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发布时间：2024-09-23 - 摘要：The free radical scavenging capacity of different extracts and macroporous resin column chromatography gradient solutions from ethanol extract of Elsholtzia rugulosa were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and improved 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) assay, with Vit C and Vit E as the control. The free radical scavenging capacity on DPPH and ABTS of different extracts of E. rugulosa followed by ethyl acetate>Vit C > n -butanol>aqueous solution>petroleum ether>chloroform and ethyl acetate>Vit E > n -butanol>aqueous solution>chloroform>petroleum ether,respectively. The free radical scavenging capacity on DPPH and ABTS of macroporous resin column chromatography gradient solutions were Vit C>50% MeOH>70% MeOH>30% MeOH>100% MeOH and 50% MeOH>70% MeOH>30% MeOH > Vit E >100% MeOH. The ethyl acetate extracts and 30%, 50% and 70%MeOH solutions exhibited strong antioxidant activity. It indicated as a new source of natural antioxidants.关键词：Elsholtzia rugulosa;2,2-diphenyl-1-picrylhydrazyl;2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt;free radical scavenging activity
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发布时间：2024-09-23 - 摘要：The free radical scavenging capacity of different extracts and macroporous resin column chroma-tography gradient solutions from ethanol extract of Elsholtzia rugulosa were determined by 2,2-diphenyl-1-picryl-hydrazyl（DPPH） and improved 2,2′-azino-bis（3-ethylbenzthiazoline-6-sulphonic acid） diammonium salt（ABTS） assay,with Vit C and Vit E as the control.The free radical scavenging capacity on DPPH and ABTS of different ex-tracts of E.rugulosa followed by ethyl acetate > Vit C > n-butanol > aqueous solution > petroleum ether > chloro-form and ethyl acetate > Vit E > n-butanol > aqueous solution > chloroform > petroleum ether,respectively.The free radical scavenging capacity on DPPH and ABTS of macroporous resin column chromatography gradient solutions were Vit C > 50% MeOH > 70% MeOH > 30% MeOH > 100% MeOH and 50% MeOH > 70% MeOH > 30% MeOH > Vit E > 100% MeOH.The ethyl acetate extracts and 30%,50% and 70% MeOH solutions exhibited strong antiox-idant activity.It indicated as a new source of natural antioxidants.关键词：Elsholtzia rugulosa;2,2-diphenyl-1-picrylhydrazyl;2,2′-azino-bis（3-ethylbenzthiazoline-6-sulphonic acid） diammonium salt;free radical scavenging activity
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发布时间：2024-09-23 - 摘要：Objective :To do the animal experiment to observe the therapeutic effect of Zhiyi Heji to children’s enuresis.Method:The water load Kidney Yang Deficiency model in rats was made by using hydrocortisone,then observing the influences upon the times of urine excreted and the amount of urine excreted caused by the Zhiyi Heji.Result:The Zhiyi Heji could obviously reduce the times of urine excreted and the amount of urine excreted in any time period and reduce the total times of urine excreted and the total amount of urine excreted in the all observation period of the water load Kidney Yang Deficiency model in rats.Conclusion:The Zhiyi Heji have a good effect to reduce the times of urine excreted and the amount of urine excreted of the water load Kidney-Yang Deficiency model in rats关键词：Zhiyi Heji;the amount of urine excreted;the times of urine excreted
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- 180
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发布时间：2024-09-23 - 摘要：Objective : To do the animal experiment to observe the therapeutic effect of Zhiyi Heji to children’s enuresis. Method : The water load Kidney Yang Deficiency model in rats was made by using hydrocortisone, then observing the influences upon the times of urine excreted and the amount of urine excreted caused by the Zhiyi Heji. Result: The Zhiyi Heji could obviously reduce the times of urine excreted and the amount of urine excreted in any time period and reduce the total times of urine excreted and the total amount of urine excreted in the all observation period of the water load Kidney Yang Deficiency model in rats. Conclusion :The Zhiyi Heji have a good effect to reduce the times of urine excreted and the amount of urine excreted of the water load Kidney-Yang Deficiency model in rats关键词：Zhiyi Heji;the amount of urine excreted;the times of urine excreted
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发布时间：2024-09-23 - 摘要：目的:探究中西药联合应用治疗口腔扁平苔藓的临床疗效。方法:将我科自2005年6月—2008年5月收治的口腔扁平苔藓患者50例,随机分为两组,西药组:用3%硼酸溶液漱口,口服利君沙0.375 g,3次/d;灭滴灵0.2 g,3次/d;维生素B和C等,每日常用量。以此处方连用7 d为1个疗程,对于有糜烂型扁平苔藓患者,可于治疗时加用激素强的松龙混悬液1mL并与1 mL 2%的利多卡因在患处于基底部做封闭注射,每隔5 d 1次或9 d 2次,连用3次为1个疗程。中西药组:在西医治疗的基础上,针对每位患者症状体征予以不同的汤药治疗,随症加减。结果:西医治疗组25例,其中痊愈4例、显效4例、好转8例、无效3例、总有效率为64%,中西药组25例:其中痊愈8例、显效6例、好转10例、无效1例、总有效率为96%,两组比较有显著差异。结论:中西药联合应用可以有效的治疗口腔扁平苔藓。关键词：中西医结合治疗;口腔扁平苔藓;临床疗效
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发布时间：2024-09-23 - 摘要：目的:分析慢性胃炎胃液细菌学分布,探讨其临床意义。方法:对慢性胃炎患者,胃镜下无菌操作采取胃液,涂片染色显微镜检查;对有感染的患者胃液进行细菌培养、分离,利用珠海黑马生物Bact-IST进行鉴定及药敏检测;用Whonet 5.0软件进行数据分析。结果:胃液镜检与细菌培养阳性率随慢性胃炎患病时间延长而增加;从胃液标本中分离出细菌阳性109例,阳性率为79.56%（109/137）;所分离到的革兰阴性杆菌占78.90%（86/109）,革兰阳性球菌占39.45%（43/109）,真菌占19.27%（21/109）;其中混合感染占27.52%（30/109）。在革兰阴性杆菌中,发酵菌占72.10%（62/86）,主要有大肠埃希菌、肺炎克雷伯菌、肠杆菌、枸橼酸杆菌;非发酵菌占27.90%（24/86）,包括绿脓假单胞菌、不动杆菌、嗜麦芽窄食假单胞菌。结论:慢性胃炎患者早期进行细菌学鉴定有助于临床治疗。关键词：慢性胃炎;胃液;细菌学分析
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- 87
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发布时间：2024-09-23 - 摘要：目的:比较罗格列酮与格列齐特对2型糖尿病患者的降糖效果及副作用。方法:56例2型糖尿病患者,随机分为2组,分别予以罗格列酮与格列齐特降糖,然后测定药后的FPG,2hPG,HbA1C的水平及观察药后的不良反应。结果:罗格列酮组总有效率高于格列齐特组（P<0.05）,且起效快（P<0.05）,不良反应发生率低于格列齐特组（P<0.05）。结论:罗格列酮与格列齐特对2型糖尿病均具有良好的疗效,但罗格列酮效果更好,安全性更高。关键词：罗格列酮;格列齐特;疗效
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发布时间：2024-09-23 - 摘要：目的:观察低剂量吸入丙酸氟替卡松加口服补肾健脾冲剂治疗儿童慢性持续期哮喘的疗效。方法:107例患儿随机分为2组,分别用低剂量吸入丙酸氟替卡松（西药组）和加用补肾健脾冲剂口服（中西药结合组）,3个月为1个疗程。观察2组患儿治疗后哮喘的控制情况、中医证候缓解情况、最大呼气峰流速、药物不良反应。结果:2组治疗1个疗程后哮喘症状明显好转,最大呼气峰流率增加量>30%,中西药结合组在改善患儿神疲纳呆、动则多汗、鼻塞流涕、易患感冒等方面,优于西药组,未发现不良反应。结论:低剂量吸入丙酸氟替卡松加补肾健脾冲剂口服可以减轻患儿气道变应性炎症,提高免疫功能,增强患儿及家长控制疾病的信心。关键词：哮喘;吸入丙酸氟替卡松;补肾健脾冲剂;临床疗效
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发布时间：2024-09-23 - 摘要：目的:观察艾迪注射液联合化疗治疗晚期肺癌的临床疗效。方法:将136例晚期肺癌患者随机分为治疗组70例和对照组66例。对照组在第1,8天给予重酒石酸长春瑞滨注射液25 mg.m-2,第1～3天给予顺铂30 mg.m-2;治疗组化疗方案同对照组,同时给予艾迪注射液50 mL加入5%葡萄糖注射液450 mL中,gtt,qd;连用10 d为1周期,每间隔21 d重复治疗,共3～4个周期。2组病例均给予格拉司琼止吐治疗。结果:近期疗效有效率治疗组为68.57%,对照组39.39%,2组比较差异有显著性（P<0.05）;生活质量改善率（等级提高）治疗组为77.14%,对照组为48.48%,治疗组优于对照组（P<0.05）;血象变化和胃肠道毒性反应比较,除厌食差异无显著性外,其余差异均具有显著性（P<0.05）,治疗组优于对照组。结论:艾迪注射液可作为一种有效的化疗增效减毒剂,配合化疗治疗中晚非小细胞期肺癌。关键词：艾迪注射液;化疗;非小细胞肺癌
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- 87
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发布时间：2024-09-23 - 摘要：Objective:Observe the effect to treat herpes zoster of early stage accompanied of heat stagnation in liver meridian with the methods to cool blood,relieve toxin and dredge collaterals combined with western medicine.Method:Seventy-two cases are divided into the treatment group（36 cases） and the control group（36 cases） at random,the conventional therapy for the treatment group is to take Chinese medicinal combined with western medicine;the conventional therapy for the control group is to apply western medicine simply,the courses are 4 weeks.Result:The effective rate of two groups is 88.89% and 61.11% respectively;the treatment group is more better than that of the control group,P < 0.05.There are no obvious side effects during the treatment courses of two groups.Conclusion:The effect to treat herpes zoster of early stage with the methods to cool blood,relieve toxin and dredge collaterals combined with western medicine is sure.关键词：method to cool blood,relieve toxin and dredge collaterals;herpes zoster
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发布时间：2024-09-23 - 摘要：Objective: Observe the effect to treat herpes zoster of early stage accompanied of heat stagnation in liver meridian with the methods to cool blood, relieve toxin and dredge collaterals combined with western medicine. Method: Seventy-two cases are divided into the treatment group (36 cases) and the control group (36 cases) at random, the conventional therapy for the treatment group is to take Chinese medicinal combined with western medicine; the conventional therapy for the control group is to apply western medicine simply,the courses are 4 weeks. Result: The effective rate of two groups is 88.89% and 61.11% respectively; the treatment group is more better than that of the control group,P<0.05. There are no obvious side effects during the treatment courses of two groups. Conclusion: The effect to treat herpes zoster of early stage with the methods to cool blood, relieve toxin and dredge collaterals combined with western medicine is sure.关键词：method to cool blood, relieve toxin and dredge collaterals;herpes zoster
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发布时间：2024-09-23 - 摘要：Objective: To review the research progress in Baoji pills’ chemical compositions and pharmacological effects in recent years, to provide bases for the future studies of Baoji pills. Method: By investigating references on Baoji pills in recent decades, the pharmacological effects of antibacterial, anti-inflammatory, analgesic, anti-diarrhea and on gastrointestinal functions of Baoji pills, the main chemical components of the single herb in the pills and the drug-pairs based on Baoji pills are summarized. Result: More than nine kind ingredients with antibacterial, anti-inflammatory effects are found the Baoji pills. More than five kinds of components which have a moderate effect of analgesic and anti-diarrhea, and for disorders of the gastrointestinal tract are also found. The drug-pairs with pharmacological enhancing effects in Baoji pills are nine pairs. Conclusion: The Baoji pills’ pharmacological effects and chemical constituents corresponds its traditional clinical applications. The recent research progress in Baoji pills will benefit the further development and utilization of the pills.关键词：Baoji pills;single herb;drug-pair;chemical component;pharmacological effect
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发布时间：2024-09-23 - 摘要：Objective:To review the research progress in Baoji pills’ chemical compositions and pharmacological effects in recent years,to provide bases for the future studies of Baoji pills.Method:By investigating references on Baoji pills in recent decades,the pharmacological effects of antibacterial,anti-inflammatory,analgesic,anti-diarrhea and on gastrointestinal functions of Baoji pills,the main chemical components of the single herb in the pills and the drug-pairs based on Baoji pills are summarized.Result:More than nine kind ingredients with antibacterial,anti-inflammatory effects are found the Baoji pills.More than five kinds of components which have a moderate effect of analgesic and anti-diarrhea,and for disorders of the gastrointestinal tract are also found.The drug-pairs with pharmacological enhancing effects in Baoji pills are nine pairs.Conclusion:The Baoji pills’pharmacological effects and chemical constituents corresponds its traditional clinical applications.The recent research progress in Baoji pills will benefit the further development and utilization of the pills.关键词：Baoji pills;single herb;drug-pair;chemical component;pharmacological effect
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- 811
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- 9
发布时间：2024-09-23 - 摘要：Medical resources of mulberry include leave,fruit,stem,and root-bark and all of them have hypoglycemic effect.The mechanisms of hypoglycemic effect include inhibiting α-glucosidase activity,promoting insulin release,increasing peripheral tissue utilization of glucose,increasing glucose uptake in liver,and inhibiting protein glycosylation.The paper reviews the present researches on hypoglycemic mechanisms for medical resources of mulberry and provides the reference for further mechanism research and future development.关键词：mulberry;Hypoglycemic effect;mechanism;α-glucosidase;insulin
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- 406
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- 21
发布时间：2024-09-23 - 摘要：Medical resources of mulberry include leave, fruit, stem, and root-bark and all of them have hypoglycemic effect. The mechanisms of hypoglycemic effect include inhibiting α-glucosidase activity, promoting insulin release, increasing peripheral tissue utilization of glucose, increasing glucose uptake in liver, and inhibiting protein glycosylation. The paper reviews the present researches on hypoglycemic mechanisms for medical resources of mulberry and provides the reference for further mechanism research and future development.关键词：mulberry;Hypoglycemic effect;mechanism;α-glucosidase;insulin
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发布时间：2024-09-23 - 摘要：In this paper,the studies on the chemical composition,qualitative and quantitative analysis,pharmacological effects of Heterosmilax yunnanensis,H.chinensis and H.gaudichaudiana,medicinal plants of Heterosmilax,were reviewed.The mixed-up clinical application of Heterosmilax with Smilax was analyzed and summarized.For utilizing Heterosmilax plants fully and for providing scientific basis of Heterosmilax,the chemical composition,physical effectiveness and mechanism of actions need to be further researched,and to reduce mix-up Heterosmila and Smilax must be distinguished from the origin.关键词：Heterosmilax;chemical composition;qualitativity;quantitativity;pharmacological effect;clinical mixed-up application
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- 351
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- 9
发布时间：2024-09-23 - 摘要：In this paper, the studies on the chemical composition, qualitative and quantitative analysis, pharmacological effects of Heterosmilax yunnanensis , H. chinensis and H. gaudichaudiana , medicinal plants of Heterosmilax , were reviewed. The mixed-up clinical application of Heterosmilax with Smilax was analyzed and summarized. For utilizing Heterosmilax plants fully and for providing scientific basis of Heterosmilax , the chemical composition, physical effectiveness and mechanism of actions need to be further researched, and to reduce mix-up Heterosmila and Smilax must be distinguished from the origin.关键词：Heterosmilax;chemical composition;qualitativity;quantitativity;pharmacological effect;clinical mixed-up application
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发布时间：2024-09-23 - 摘要：Based on the complex chemical structures and various biological activities, diterpenoid alkaloids demonstrate the importance for the development of new drugs. The main activities and toxicities of diterpenoid alkaloids are summarized in this paper.关键词：diterpenoid alkaloids;immunohistochemistry;methylene blue staining;toxicity determination;turbidimetric method
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发布时间：2024-09-23 - 摘要：Based on the complex chemical structures and various biological activities,diterpenoid alkaloids demonstrate the importance for the development of new drugs.The main activities and toxicities of diterpenoid alkaloids are summarized in this paper.关键词：diterpenoid alkaloids;immunohistochemistry;methylene blue staining;toxicity determination;turbidimetric method
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- 820
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- 30
发布时间：2024-09-23 - 摘要：The rhizomes of Cyperus rotundus is a traditional chinese medicine for the treatment of dispersing the depressed liver-energy, regulating vital energy, dysmenorrhea, and menstrual disorders and so on. Modern pharmacological studies have proven the broad range of biological activities, such as antidepressant, anti-cancer, acetylcholinesterase inhibitory activity, anti-diabetic. Previous phytochemical investigations have described the presence of more than 100 compounds in it, among of which about 70 ones are sesquiterpenes with diverse skeletons such as eudesmane, patchoulane, copane, rotundane and so on. This paper reviews the progress of the constituents of it which provides references for further development and utilization of it.
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发布时间：2024-09-23 - 摘要：The rhizomes of Cyperus rotundus is a traditional chinese medicine for the treatment of dispersing the depressed liver-energy,regulating vital energy,dysmenorrhea,and menstrual disorders and so on.Modern pharmacological studies have proven the broad range of biological activities,such as antidepressant,anti-cancer,acetylcholinesterase inhibitory activity,anti-diabetic.Previous phytochemical investigations have described the presence of more than 100 compounds in it,among of which about 70 ones are sesquiterpenes with diverse skeletons such as eudesmane,patchoulane,copane,rotundane and so on.This paper reviews the progress of the constituents of it which provides references for further development and utilization of it.
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- 1978
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- 80
发布时间：2024-09-23 - 摘要：Sophoridine（sophoridine,SR） is a kind of monomer alkaloid which is purified from Sophora alopecuroides L.The researchers found that it has many benefits for heaeth,such as anti-tumor,anti-arrhythmia,anti-inflammatory antibacterial,sperm suppression in vitro,immune suppression and so on.As a kind of highly efficient low toxicity anti-cancer alkaloid,sophoridine is applied in clinical treatment of malignant lymphoma and gastrointestinal tumors.In order to provied references for further development and application,the main pharmacological effects of sophoridine are reviewed in this paper.关键词：pharmacological studies;progress;review
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- 712
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发布时间：2024-09-23 - 摘要：Sophoridine (sophoridine, SR) is a kind of monomer alkaloid which is purified from Sophora alopecuroides L.The researchers found that it has many benefits for heaeth, such as anti-tumor, anti-arrhythmia, anti-inflammatory antibacterial, sperm suppression in vitro, immune suppression and so on. As a kind of highly efficient low toxicity anti-cancer alkaloid, sophoridine is applied in clinical treatment of malignant lymphoma and gastrointestinal tumors. In order to provied references for further development and application, the main pharmacological effects of sophoridine are reviewed in this paper.关键词：pharmacological studies;progress;review
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发布时间：2024-09-23 - 摘要：该文认为方证辨证具有操作简捷方便,辨证精确高效,适应范围广阔,解决方证分离,提供科研思路等5大优势。同时也指出方证辨证存在摒弃辨证体系,违反辨证程序,证名表述不清,容易对号入座、方证数量庞杂等不足之处。认为方证辨证在研究不太成熟的今天,还不能成为一种正式的辨证方法,只能作为辨证诊断后选择相应方药的补充。关键词：方证辨证;临床意义;褒贬评述
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- 252
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- 5
发布时间：2024-09-23 - 摘要：Based on the investigation and research of literature,the author finds there are some errors in the Chinese Pharmacopoeia need errata and some questions need discussion.关键词：Chinese Pharmacopoeia Volume I;2010 edition;discussion
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- 210
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- 5
发布时间：2024-09-23 - 摘要：Based on the investigation and research of literature, the author finds there are some errors in the Chinese Pharmacopoeia need errata and some questions need discussion.关键词：Chinese Pharmacopoeia Volume I;2010 edition;discussion
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发布时间：2024-09-23 - 摘要：<正>痔是常见病、多发病,任何年龄皆可发病。据有关文献报道,痔疮患者约占受检人群的46.3%[1]。本科收集三期内痔患者300例,采用"消痔灵"液以四步注射治疗,取得了较为满意的临床疗效。1资料与方法1.1一般资料300例病人均为北京市鼓楼中医医院肛肠科2007年1月～2009年12月期间的门诊患者。其中男性174例,占58%,女性126例,占42%;年龄19～72岁,平均年龄47岁;病程1～30年,平均病史4年。
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发布时间：2024-09-23 - 摘要：<正>支气管哮喘是儿科临床常见的慢性呼吸道疾病,以持续咳喘或咳喘反复发作为特征,近年来发病率呈上升趋势,严重影响患儿的身心健康。支气管哮喘中西医结合治疗的指导思想是:发时治标、平时治本、标本兼治的原则。我院采用中西医结合治疗小儿支气管哮喘发作及减少复发方面取得了较满意的疗效。
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- 95
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发布时间：2024-09-23 - 摘要：<正>围绝经期综合征是女性在围绝经期和绝经期,因卵巢功能衰退至消失而表现的一组内分泌失调和植物神经功能紊乱的综合症状。在治疗上西医多以激素替代疗法为主,但临床应用有局限性,且长期服用又有诱发子宫内膜癌、乳腺癌的危险。作者运用中医理论辨证治疗129例,疗效满意。
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- 170
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发布时间：2024-09-23 - 摘要：<正>痤疮是一种青春期常见的毛囊皮脂腺慢性炎症性皮肤病,发病机制复杂,许多研究表明,皮脂分泌过多,毛囊过度角化,痤疮丙酸杆菌增殖,过度免疫反应,遗传及雄激素受体增多等都起了重要作用[1]。在我科长期的临床诊疗中,运用自拟中药枇杷去渣汤内服治疗取得了较为满意的疗效。
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术
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发布时间：2024-09-23
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