最新刊期
卷 16 ， 期 14 ， 2010
- 摘要：Objective: To study the influence of differenet pH on the oil-water partition co-efficient,transdermal absorption characteristics and the anti-inflammation effect of brucine solution. Method: The oil-water partition co-efficients and the permeation rates of brucine in pH 5, 7.4 and 9 phosphate buffer solution(PBS) were measured; The effects of brucine in pH5, 7.4 and pH 9 on the inflammatory edema of ear induced by xylol in mice were also determined. Result: The oil-water partition coefficient and permeation rate of brucine in pH 9 were(22.17±0.4) and (2.84±0.10)μg ·cm-2 ·h-1,which was almost 239.5 times and 3.15 times more than the corresponding data obtained under pH 5 PBS. The effect of inhibiting the inflammatory edema in the ear of mice induced by xylol was also markly increased under pH 9. Conclusion: The oil-water partition coefficient and transdermal absorption characteristics of brucine can be improved by increasing pH. which also markly promotes the anti-inflammatory activities of brucine.关键词：brucine;oil-water partition coefficiency;anti-inflammation;transdermal absorption
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the influence of differenet pH on the oil-water partition co-efficient,trans- dermal absorption characteristics and the anti-inflammation effect of brucine solution. Method:The oil-water parti- tion co-efficients and the permeation rates of brucine in pH 5,7. 4 and 9 phosphate buffer solution （ PBS） were measured; The effects of brucine in pH5,7. 4 and pH 9 on the inflammatory edema of ear induced by xylol in mice were also determined. Result:The oil-water partition coefficient and permeation rate of brucine in pH 9 were （22. 17 ± 0. 4） and （2. 84 ± 0. 10）μg·cm -2·h -1,which was almost 239. 5 times and 3. 15 times more than the cor- responding data obtained under pH 5 PBS. The effect of inhibiting the inflammatory edema in the ear of mice in- duced by xylol was also markly increased under pH 9. Conclusion:The oil-water partition coefficient and transder- mal absorption characteristics of brucine can be improved by increasing pH. which also markly promotes the anti-in- flammatory activities of brucine.关键词：brucine;oil-water partition coefficiency;anti-inflammation;transdermal absorption
- 0
- |
- 184
- |
- 7
发布时间：2024-09-23 - 摘要：Objective: To study the optimal technics of the extraction of Gegen Qinlian dispensing granule. Method: Taking puerarin, baicalin and berberine as the index, a method of orthogonal experimental design was applied to optimizing the technics of extraction. Result: The optimal extraction technology of Gegen Qinlian dispensing granule was to extract Radix Puerariae Lobatae, Radix Scutellariae, and Radix et Rhizoma Glycyrrhizae Praeparata cum Melle together and to extract Rhizoma Coptidis alone. Radix Puerariae Lobatae, Radix Scutellariae, and Radix et Rhizoma Glycyrrhizae Praeparata cum Melle were weighed in proportion, and were extracted for 1.5 h twice with 10 times and 8 times water, respectively. Rhizoma Coptidis was weighed and extracted for 1.5 h twice with 12 times and 10 times water, respectively. Conclusion: The extraction of the active components of puerarin, baicalin and berberine from Gegen Qinlian dispensing granule was satisfactory.关键词：Gegen Qinlian dispensing granule;technics of extraction;optimal
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the optimal technics of the extraction of Gegen Qinlian dispensing granule.Method:Taking puerarin,baicalin and berberine as the index,a method of orthogonal experimental design was ap-plied to optimizing the technics of extraction.Result:The optimal extraction technology of Gegen Qinlian dispensing granule was to extract Radix Puerariae Lobatae,Radix Scutellariae,and Radix et Rhizoma Glycyrrhizae Praeparata cum Melle together and to extract Rhizoma Coptidis alone.Radix Puerariae Lobatae,Radix Scutellariae,and Radix et Rhizoma Glycyrrhizae Praeparata cum Melle were weighed in proportion,and were extracted for 1.5 h twice with 10 times and 8 times water,respectively.Rhizoma Coptidis was weighed and extracted for 1.5 h twice with 12 times and 10 times water,respectively.Conclusion:The extraction of the active components of puerarin,baicalin and berberine from Gegen Qinlian dispensing granule was satisfactory.关键词：Gegen Qinlian dispensing granule;technics of extraction;optimal
- 0
- |
- 1327
- |
- 15
发布时间：2024-09-23 - 摘要：Objective: To optimize the extraction technology for Sanmiao Xiaotong Tie. Method: An orthogonal test was adopted in this study. The extraction technology of San Miao Xiao Tong Tie was optimized by extracting berberine hydrochloric and swelling function of mice. Result: The best extraction technology was to extract for 2 times with 10 fold ethanol solution (50%) and 2 h each time. Conclusion: The optimized extraction technology was rational and the extraction rate of the active components was high.关键词：orthogonal test;extraction technology;berberine hydrochloric;mouse ear swelling
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To optimize the extraction technology for Sanmiao Xiaotong Tie.Method:An or-thogonal test was adopted in this study.The extraction technology of San Miao Xiao Tong Tie was optimized by ex-tracting berberine hydrochloric and swelling function of mice.Result:The best extraction technology was to extract for 2 times with 10 fold ethanol solution（50%） and 2 h each time.Conclusion:The optimized extraction technolo-gy was rational and the extraction rate of the active components was high.关键词：orthogonal test;extraction technology;berberine hydrochloric;mouse ear swelling
- 0
- |
- 70
- |
- 2
发布时间：2024-09-23 - 摘要：Objective: To study the optimum ethanol extraction process and antioxidant activities in vitro of Scutellaria barbata D.Don. Method: The optimum ethanol extraction process was established with the orthogonal design. The extraction rate of extractum, total flavonoids and scutellarin from crude material were chosen as the assessment guideline. Antioxidant activities in vitro of the extract were evaluated by FRAP assay and DPPH assay compared with Trolox. Result: The optimum ethanol extraction process conditions were as follows: 10 fold of 75% ethanol was added, to be extracted 3 times by reflux and 1 h per time. The extract of S. barbata exerted antioxidant role with dose-dependent relation in the two systems through oxidation-reduction reaction and inhibition of free radical. Conclusion: Validation studies proved that the ethanol extraction process had good reproducibility and feasibility. The ethanol extract of S. barbata exhibited a strong antioxidant activity in vitro , which is worthy of further studies.关键词：Scutellaria barbata;Orthogonal Design;total flavonoids;scutellarin;antioxidant activities;FRAP assay;DPPH assay
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the optimum ethanol extraction process and antioxidant activities in vitro of Scutellaria barbata D.Don.Method:The optimum ethanol extraction process was established with the orthogonal design.The extraction rate of extractum,total flavonoids and scutellarin from crude material were chosen as the as-sessment guideline.Antioxidant activities in vitro of the extract were evaluated by FRAP assay and DPPH assay compared with Trolox.Result:The optimum ethanol extraction process conditions were as follows:10 fold of 75% ethanol was added,to be extracted 3 times by reflux and 1 h per time.The extract of S.barbata exerted antioxidant role with dose-dependent relation in the two systems through oxidation-reduction reaction and inhibition of free radi-cal.Conclusion:Validation studies proved that the ethanol extraction process had good reproducibility and feasibil-ity.The ethanol extract of S.barbata exhibited a strong antioxidant activity in vitro,which is worthy of further stud-ies.关键词：Scutellaria barbata;Orthogonal Design;total flavonoids;scutellarin;antioxidant activities;FRAP assay;DPPH assay
- 0
- |
- 381
- |
- 12
发布时间：2024-09-23 - 摘要：Objective: The preparation process optimization of microns red Ginseng was performed with detection of ginsenoside. Method: The single factor law on the factors affected red Ginseng microns in diameter screening, particle size distribution to optimize the production of red Ginseng microns optimum technology. The ginsenoside Rg1, Re and Rb1 were determined by HPLC. Result: The best process was as follows: the wind bag length was 20 cm, crushing time 40 min into the charge for a single 500 g; the concent of ginsenoside Rg1, Re, Rb1 were 20.6, 17.7, 24.1 mg ·g-1 respectively. Ginsenosides from micron Red Ginseng was significantly higher than normal powder and sliced Red Ginseng . Conclusion: Micron Red Ginseng preparation technology was stable. The content determination method established can be used for determination of ginsenosides composition in micron Red Ginseng .关键词：ultra-fine pulverizing;ginsenoside;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:The preparation process optimization of microns red Ginseng was performed with de-tection of ginsenoside.Method:The single factor law on the factors affected red Ginseng microns in diameter screening,particle size distribution to optimize the production of red Ginseng microns optimum technology.The gin-senoside Rg1,Re and Rb1 were determined by HPLC.Result:The best process was as follows:the wind bag length was 20 cm,crushing time 40 min into the charge for a single 500 g;the concent of ginsenoside Rg1,Re,Rb1 were 20.6,17.7,24.1 mg.g-1 respectively.Ginsenosides from micron Red Ginseng was significantly higher than normal powder and sliced Red Ginseng.Conclusion:Micron Red Ginseng preparation technology was stable.The content determination method established can be used for determination of ginsenosides composition in micron Red Ginseng.关键词：ultra-fine pulverizing;ginsenoside;HPLC
- 0
- |
- 129
- |
- 3
发布时间：2024-09-23 - 摘要：Objective: To optimize extraction process of the combination of Radix Bupleuri and Paeoniae Alba. Method: The proper extracting condition was optimized by orthogonal tests with the amount of saikosaponin A, D and paeoniflorin as index. Result: The proper extracting condition was that alcohol concentration was 70 percent, and extracted with 10 fold alcohol for 2 times, 1.5 hour each time. Conclusion: Optimized process condition was scientific, stable and feasible.关键词：herbal pair;Radix Bupleuri-paeoniae Alba;orthogonal tests;extraction process
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To optimize extraction process of the combination of Radix Bupleuri and Paeoniae Alba.Method:The proper extracting condition was optimized by orthogonal tests with the amount of saikosaponin A,D and paeoniflorin as index.Result:The proper extracting condition was that alcohol concentration was 70 per-cent,and extracted with 10 fold alcohol for 2 times,1.5 hour each time.Conclusion:Optimized process condition was scientific,stable and feasible.关键词：herbal pair;Radix Bupleuri-paeoniae Alba;orthogonal tests;extraction process
- 0
- |
- 448
- |
- 10
发布时间：2024-09-23 - 摘要：目的:对金芍胶囊的水提取工艺进行优选。方法:以方中君药赤芍中芍药苷转移率及提取出膏率为评价指标,用RP-HPLC测定其含量,采用正交表设计试验法对处方水提取工艺进行优选。结果:最佳水提取工艺条件为按处方比例取药材加水煎煮2次（第1次10倍量,第2次8倍量）,每次1 h。结论:该工艺芍药苷提取率较高,有较好的稳定性,且工艺简便经济,适合于工业生产。关键词：金芍胶囊;赤芍;芍药苷;提取工艺
- 0
- |
- 135
- |
- 6
发布时间：2024-09-23 - 摘要：Objective:To optimize the extraction condition of lowering blood sugar substance from agrimony.Method:With effects of lowering blood sugar as criteria,concentration of alcohol,extraction time,and the ratio of gardenia to liquor and extraction temperature are evaluated by L9（34） orthogonal design method.Result:The opti-mum extraction condition was described as follow:hot reflux extraction 3 times（once 1 hour）,60% ethanol solu-tion,and 10-fold solvent.Conclusion:The high extraction rate is got at the optimum technology.关键词：agrimony;lowering blood sugar substance;reflux
- 0
- |
- 232
- |
- 5
发布时间：2024-09-23 - 摘要：Objective: To optimize the extraction condition of lowering blood sugar substance from agrimony. Method: With effects of lowering blood sugar as criteria,concentration of alcohol,extraction time,and the ratio of gardenia to liquor and extraction temperature are evaluated by L9(34) orthogonal design method. Result: The optimum extraction condition was described as follow: hot reflux extraction 3 times(once 1 hour),60% ethanol solution,and 10-fold solvent. Conclusion: The high extraction rate is got at the optimum technology.关键词：agrimony;lowering blood sugar substance;reflux
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:考察白藜芦醇固体脂质纳米粒（Res-SLN）的制备工艺及Res-SLN的形态学。方法:采用HPLC测定制剂中白藜芦醇（Res）的含量;采用单因素考察法筛选载体、稳定剂及制备工艺。结果:建立了用HPLC测定本制剂中Res含量,采用单硬脂酸甘油酯（GMS）作为载体材料,泊洛沙姆188（F68）稳定剂,采用热高压均质法制备得到Res-SLN,平均粒径为125 nm,平均包封率为91%,平均载药量为10%。结论:热高压均质法制备SLN,包封率高、简便可行。关键词：白藜芦醇;固体脂质纳米粒;制备工艺;形态学
- 0
- |
- 379
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: To explore the optimal purification process of the polysaccharides from sugarcane biogases. Method: To explore the optimum technology, we compared Savage method with TCA method for deproteinization and hydrogen peroxide solution method with active carbon method for decoloration. And four common macroporous absorption resins were compared for differences in adsorption and desorption performance. Then phenol-sulfuric acid method was used to determine the content of the polysaccharides from sugarcane biogases. Result: The optimum purification technological conditions were determined as following: deproteinization with savage method; discoloration with active carbon method; purification with macroporous absorption resins of MG-1 (the upper sample weight is 0.3 g; the column high is 35 cm and the flow rate is 10 mL/15 min). The polysaccharides of sugarcane biogases improved from 51.11% to 83.57%. Conclusion: This experiment provided a reference for the purification of polysaccharides from sugarcane biogases and provided an evidence for the further development of sugarcane biogases.关键词：polysaccharides from sugarcane biogases;deproteinization;decoloration;macroporous absorption resins;purification process
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To explore the optimal purification process of the polysaccharides from sugarcane biogases.Method:To explore the optimum technology,we compared Savage method with TCA method for depro-teinization and hydrogen peroxide solution method with active carbon method for decoloration.And four common macroporous absorption resins were compared for differences in adsorption and desorption performance.Then phe-nol-sulfuric acid method was used to determine the content of the polysaccharides from sugarcane biogases.Result:The optimum purification technological conditions were determined as following:deproteinization with savage meth-od;discoloration with active carbon method;purification with macroporous absorption resins of MG-1（the upper sample weight is 0.3 g;the column high is 35 cm and the flow rate is 10 mL /15 min）.The polysaccharides of sug-arcane biogases improved from 51.11% to 83.57%.Conclusion:This experiment provided a reference for the pur-ification of polysaccharides from sugarcane biogases and provided an evidence for the further development of sugar-cane biogases.关键词：polysaccharides from sugarcane biogases;deproteinization;decoloration;macroporous absorp-tion resins;purification process
- 0
- |
- 430
- |
- 10
发布时间：2024-09-23 - 摘要：Objective: To determine the trace elements in traditional Chinese Medicine of ophicslcite and its decoction, and the primary speciation analysis on the trace elements of Ophicalcitum. Method: ICP-MS was used to determine the content of 13 trace elements and their primary speciation also been studied. Result: The content of elements in the residue was far more than the content in the solution. The content of elements in original drug was as follows: Mg>Ca>Fe>K>Zn>Mn>Cu, among which Se, Cu, Mo, K were in a higher extraction rate. Conclusion : The mineral medicine decoction extraction rate and the leaching residue ratio were relatively smaller than botanical drug. In ophicslcite, Se, Cu could be considered to be the two largest elements of the role of the drug or the most characteristic elements.关键词：ophicalcitum;ICP-MS;mineral elements;primary speciation
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To determine the trace elements in traditional Chinese Medicine of ophicslcite and its decoction,and the primary speciation analysis on the trace elements of Ophicalcitum.Method:ICP-MS was used to determine the content of 13 trace elements and their primary speciation also been studied.Result:The content of elements in the residue was far more than the content in the solution.The content of elements in original drug was as follows:Mg > Ca > Fe > K > Zn > Mn > Cu,among which Se,Cu,Mo,K were in a higher extraction rate.Conclu-sion:The mineral medicine decoction extraction rate and the leaching residue ratio were relatively smaller than bo-tanical drug.In ophicslcite,Se,Cu could be considered to be the two largest elements of the role of the drug or the most characteristic elements.关键词：ophicalcitum;ICP-MS;mineral elements;primary speciation
- 0
- |
- 198
- |
- 16
发布时间：2024-09-23 - 摘要：Objective:To study on the chemical chromatographic corresponding methods in different botani-cal origins of Sinapis semen.Method:The presence of 4-hydroxybenzylglucosinolate,4-hydroxy benzoylcholine and sinapine was identified by TLC and HPLC,respectively.Result:The TLC and HPLC chromatograms showed the obvious differences between the two origins,while three standards were identified in Sinapis alba,and only sinapine was detected in Brassica juncea.Conclusion:The established methods were simple and can be used for evaluating the quality in different botanical origins of Sinapis semen.关键词：Sinapis alba;Brassica juncea;corresponding identification;TLC;HPLC
- 0
- |
- 231
- |
- 6
发布时间：2024-09-23 - 摘要：Objective : To study on the chemical chromatographic corresponding methods in different botanical origins of Sinapis semen. Method : The presence of 4-hydroxybenzylglucosinolate, 4-hydroxy benzoylcholine and sinapine was identified by TLC and HPLC, respectively. Result : The TLC and HPLC chromatograms showed the obvious differences between the two origins, while three standards were identified in Sinapis alba , and only sinapine was detected in Brassica juncea . Conclusion : The established methods were simple and can be used for evaluating the quality in different botanical origins of Sinapis semen.关键词：Sinapis alba;Brassica juncea;corresponding identification;TLC;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for determination of different parts of Arctium tomento-sum.Method:Chromatographic separation was achieved by using RP-HPLC system consisting of a Shimadzu LC-6AD and Kromasil C18column（4.6 mm × 250 mm,5 μm）.The mobile phase was consisted of methanol and water（55∶ 45）.Detection wavelength was at 280 nm.The flow rate was set at 1.0 mL.min-1.Result:A small amount of arctiin and no arctigenin was found in the root;while stem and leaf nearly did not contain.Leaves and inflores-cence did not contain arctiin and a few arctigenin.But both ingredients were higher in the fruit than in the leaves and inflorescence.Conclusion:The method was simple and accurate,and it could be used for simultaneous deter-mination of arctiin and arctigenin from Radix Arctium,to provide the basis for a better development of A.tomento-sum Mill.关键词：RP-HPLC;Arctium tomentosum Mill.;arctiin;arctigenin
- 0
- |
- 192
- |
- 13
发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for determination of different parts of Arctium tomentosum . Method: Chromatographic separation was achieved by using RP-HPLC system consisting of a Shimadzu LC-6AD and Kromasil C18 column (4.6 mm×250 mm,5 μm). The mobile phase was consisted of methanol and water (55 ∶45). Detection wavelength was at 280 nm. The flow rate was set at 1.0 mL ·min-1. Result: A small amount of arctiin and no arctigenin was found in the root; while stem and leaf nearly did not contain. Leaves and inflorescence did not contain arctiin and a few arctigenin. But both ingredients were higher in the fruit than in the leaves and inflorescence. Conclusion: The method was simple and accurate, and it could be used for simultaneous determination of arctiin and arctigenin from Radix Arctium, to provide the basis for a better development of A. tomentosum Mill.关键词：RP-HPLC;Arctium tomentosum Mill.;arctiin;arctigenin
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To establish a quality standard for Three Patches of Hushi. Method: Helleborus thibetanus , Rhizoma Corydalis , Clematis chinensis Osbeck were identified by TLC, and the content of icariin was determined by HPLC. Result: Helleborus thibetanus , Rhizoma Corydalis , Clematis chinensis Osbeck could be identified by TLC, icariin was linear in the range of 0.195 2- 1.659 2 μg,r=0.999 8. The average recovery was 96.29%, RSD 1.05%(n=9). Conclusion: This method was accurate and can be used for the quality control of Three Patches of Hushi.关键词：Three Patches of Hushi;TLC;HPLC;icariin
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a quality standard for Three Patches of Hushi.Method:Helleborus thi-betanus,Rhizoma Corydalis,Clematis chinensis Osbeck were identified by TLC,and the content of icariin was de-termined by HPLC.Result:Helleborus thibetanus,Rhizoma Corydalis,Clematis chinensis Osbeck could be identi-fied by TLC,icariin was linear in the range of 0.195 2-1.659 2 μg,r = 0.999 8.The average recovery was 96.29%,RSD 1.05%（n = 9）.Conclusion:This method was accurate and can be used for the quality control of Three Patches of Hushi.关键词：Three Patches of Hushi;TLC;HPLC;icariin
- 0
- |
- 106
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a high performance capillary electrophoresis（HPCE） method for the de-termination of hesperidin,glycyrrhizic acid and glycyrrhetinic acid in Erchen Tang.Method:Fused silica capillary coated（50 μm × 95 cm） with detection window at 86.5 cm from anode was employed and 50 mmol.L-1 borax solu-tion（10% acetonitrile,pH 9.28） served as the running buffer.Other conditions were as follows:electrokinetic in-jection 20 s.analytical voltage 24 kV,detection wavelength 254 nm and temperature 20 ℃.Result:The linearity of hesperidin was in the range of 0.10-2.40 g.L-1（r = 0.999 4）,the average recovery was 98.17% and RSD1.49%.The linearity of glycyrrhizic acid was in the range of 0.04-0.72 g.L-1（r = 0.999 4）,the average recovery was 98.72% and RSD 2.95%.The linearity of glycyrrhetinic acid was in the range of 0.01-0.18 g.L-1（r = 0.999 6）,the average recovery was 98.48% and RSD 2.25%.Conclusion:The method is rapid,simple and accurate and it provides the basis of the fingerprints of CE and composite herbal medicines.关键词：Erchen Tang;high performance capillary electrophoresis;hesperidin;glycyrrhizic acid;gly-cyrrhetinic acid
- 0
- |
- 147
- |
- 8
发布时间：2024-09-23 - 摘要：Objective: To establish a high performance capillary electrophoresis(HPCE) method for the determination of hesperidin, glycyrrhizic acid and glycyrrhetinic acid in Erchen Tang. Method: Fused silica capillary coated (50 μm×95 cm) with detection window at 86.5 cm from anode was employed and 50 mmol ·L-1 borax solution (10% acetonitrile, pH 9.28) served as the running buffer. Other conditions were as follows: electrokinetic injection 20 s. analytical voltage 24 kV, detection wavelength 254 nm and temperature 20 ℃. Result: The linearity of hesperidin was in the range of 0.10- 2.40 g ·L-1 (r=0.999 4), the average recovery was 98.17% and RSD1.49%. The linearity of glycyrrhizic acid was in the range of 0.04- 0.72 g ·L-1 (r=0.999 4), the average recovery was 98.72% and RSD 2.95%. The linearity of glycyrrhetinic acid was in the range of 0.01- 0.18 g ·L-1(r=0.999 6), the average recovery was 98.48% and RSD 2.25%. Conclusion: The method is rapid, simple and accurate and it provides the basis of the fingerprints of CE and composite herbal medicines.关键词：Erchen Tang;high performance capillary electrophoresis;hesperidin;glycyrrhizic acid;glycyrrhetinic acid
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 -
Comparison of Acetyl-11-keto--boswellic Acid Contents in Olibanum before and after Processed by HPLC
摘要：Objective: To compare the changes of contents of acetyl-11-keto-β-boswellic acid in Olibanum before and after processed. Method: HPLC was used for the comparison. Result: Acetyl-11-keto-β-boswellic acid contents in the different batch of Olibanum beforeand after processed were as follows raw 3.79%, stir-fried 4.07%, vinegar processed 4.08% (batch 090325); raw 3.24%, stir-fried 3.32%, vinegar processed 3.71% (batch 090917); raw 4.02%, stir-fried 4.07%, vinegar processed 4.08% (batch 100413). Conclusion: Acetyl-11-keto-β-boswellic acid content in Olibanum was increased after processing.关键词：Olibanum;processing;acetyl-11-keto-β-boswellic acid;HPLC- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To compare the changes of contents of acetyl-11-keto-β-boswellic acid in Olibanum before and after processed. Method: HPLC was used for the comparison. Result: Acetyl-11-keto-β-boswellic acid contents in the different batch of Olibanum beforeand after processed were as follows raw 3. 79% ,stir-fried 4. 07% , vinegar processed 4. 08% （ batch 090325 ）; raw 3. 24% ,stir-fried 3. 32% ,vinegar processed 3. 71% （ batch 090917）; raw 4. 02% ,stir-fried 4. 07% ,vinegar processed 4. 08% （batch 100413）. Conclusion: Acetyl-11-ke- to-β-boswellic acid content in Olibanum was increased after processing.关键词：Olibanum;processing;acetyl-11-keto-β-boswellic acid;HPLC
- 0
- |
- 298
- |
- 8
发布时间：2024-09-23 - 摘要：Objective:To develop an HPLC method for determination of Buddleoside from Flos Chrysanthemi Indici. The result was compared with IR spectrums. Method:Flos Chrysanthemi Indici from various habitats were used as test sample and HPLC was used. The chromatographic separation was performed on a Diamonsil C18（4. 6 mm × 250 mm,5 μm） column gradient eluted with a mobile phase of 0. 5% H3PO4（A）-acetonitrile（B）,B changed from 10% to 20% in the first 10 min,in the next 20 min,when B changed from 20% to 30% ,and in the last 10 min,B changed to 40% . The detection wavelength was set at 326 nm and column temperature was set at 35 ℃ . The FTIR spectrums,the second-order derivative spectrogram and the two-dimensional infrared correlation spectrograms were used. Result:Buddleoside was linear within the range of 0. 262-5. 24 μg （r = 0. 999 91）. The average recovery was 98. 75% ,with RSD 1. 02% （n = 5）. There were differences of samples on the second-order derivative spectrogram of Flos Chrysanthemi Indici. in 1 700-1 400 cm -1 and the two-dimensional infrared correlation spectrograms could be classified between frequency range 1 800-800 cm -1. Conclusion:The method was accurate,repetable and suitable to determine buddleoside from Flos Chrysanthemi Indici. The method can be used for quality control of Flos Chrysanthe- mi Indici. IR spectrum can also be used to identify the flavonoids from Flos Chrysanthemi Indici.关键词：HPLC;Fourier transform infrared spectroscopy;Flos Chrysanthemi Indici;buddleoside
- 0
- |
- 340
- |
- 8
发布时间：2024-09-23 - 摘要：Objective: To develop an HPLC method for determination of Buddleoside from Flos Chrysanthemi Indici. The result was compared with IR spectrums. Method: Flos Chrysanthemi Indici from various habitats were used as test sample and HPLC was used. The chromatographic separation was performed on a Diamonsil C18 (4.6 mm×250 mm,5 μm) column gradient eluted with a mobile phase of 0.5%H3PO4(A)-acetonitrile(B), B changed from 10% to 20% in the first 10 min, in the next 20 min, when B changed from 20% to 30%, and in the last 10 min, B changed to 40%. The detection wavelength was set at 326 nm and column temperature was set at 35 ℃. The FTIR spectrums, the second-order derivative spectrogram and the two-dimensional infrared correlation spectrograms were used. Result: Buddleoside was linear within the range of 0.262- 5.24 μg (r=0.999 91). The average recovery was 98.75%, with RSD 1.02% (n=5). There were differences of samples on the second-order derivative spectrogram of Flos Chrysanthemi Indici. in 1 700- 1 400 cm-1 and the two-dimensional infrared correlation spectrograms could be classified between frequency range 1 800- 800 cm-1. Conclusion: The method was accurate, repetable and suitable to determine buddleoside from Flos Chrysanthemi Indici. The method can be used for quality control of Flos Chrysanthemi Indici. IR spectrum can also be used to identify the flavonoids from Flos Chrysanthemi Indici.关键词：HPLC;Fourier transform infrared spectroscopy;Flos Chrysanthemi Indici;buddleoside
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To establish a dual-wavelength HPLC method for the determination of ursolic acid and loganin from Cornus officinalis. Method: Octadecyl bonded silica stationary phase Shim-pack VP-ODS column was used. Gradient elution was performed with acetonitrile as mobile phase A, 0.02% phosphoric acid water as mobile phase B. The detection wavelength was set at 210 nm and 238 nm. Result: UA was linear in the range of 0.1- 2.0 μg, (r=0.999 9). The average recovery was 99.44% (RSD 0.39%, n=6). Loganin was linear in the range of 0.08- 1.6 μg. (r=0.999 9). The average recovery was 98.92% (RSD 0.75%, n=6). Conclusion: The method established was simple, rapid and accurate and could be used for simultaneous determination of ursolic acid and loganin from dogwood.关键词：HPLC;ursolic acid;loganin;Cornus officinalis;content
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a dual-wavelength HPLC method for the determination of ursolic acid and loganin from Cornus officinalis.Method:Octadecyl bonded silica stationary phase Shim-pack VP-ODS column was used.Gradient elution was performed with acetonitrile as mobile phase A,0.02% phosphoric acid water as mo-bile phase B.The detection wavelength was set at 210 nm and 238 nm.Result:UA was linear in the range of 0.1-2.0 μg,（r = 0.999 9）.The average recovery was 99.44%（RSD 0.39%,n = 6）.Loganin was linear in the range of 0.08-1.6 μg.（r = 0.999 9）.The average recovery was 98.92%（RSD 0.75%,n = 6）.Conclusion:The method established was simple,rapid and accurate and could be used for simultaneous determination of ursolic acid and loganin from dogwood.关键词：HPLC;ursolic acid;loganin;Cornus officinalis;content
- 0
- |
- 252
- |
- 6
发布时间：2024-09-23 - 摘要：Objective: To establish the quality standard for Niaoganning Granule. Method: The TLC was applied to identify Herba Glechomae Longitubae and Herba Pteridis Nervosae in this prescription, and aesculetin content in Herba Violae was determined by HPLC. A column (4.6 mm×250 mm ,5 μm) was used with the mobile phase of methanol and 0.5% phosphoric acid (22 ∶78) at the detection wavelength of 345 nm. Result: Glechomae Longitubae and Pteridis Nervosae could be identified by TLC. The aesculetin linear rang of 20.08-2 080 ng, and the average recoverywas 96.3%,RSD 1.9%, Conclusion: The above methods are simple, sensitive, accurate and reproducible, and can be used for the quality control of this granule.关键词：Glechomae Longitubae;Pteridis Nervosae;Violae;aesculetin;TLC;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish the quality standard for Niaoganning Granule.Method:The TLC was applied to identify Herba Glechomae Longitubae and Herba Pteridis Nervosae in this prescription,and aesculetin content in Herba Violae was determined by HPLC.A column（4.6 mm × 250 mm,5 μm） was used with the mobile phase of methanol and 0.5% phosphoric acid（22∶ 78） at the detection wavelength of 345 nm.Result:Glechomae Longitubae and Pteridis Nervosae could be identified by TLC.The aesculetin linear rang of 20.08-2 080 ng,and the average recoverywas 96.3%,RSD 1.9%,Conclusion:The above methods are simple,sensitive,accurate and re-producible,and can be used for the quality control of this granule.关键词：Glechomae Longitubae;Pteridis Nervosae;Violae;aesculetin;TLC;HPLC
- 0
- |
- 164
- |
- 1
发布时间：2024-09-23 - 摘要：Objective:To investigate the experimental proof for the application of super fine crushing tech-nique（SFCT） to the traditional Chinese medicinal herb and develop new methods to evaluate the effect of SFCT on drug.Method:The dissolution of Rhodiola rosea prepared by different techniques was investigated by dialysis deter-mining gallic acid,salidroside and catechin as the test marker.These markers were determined at different time by RP-HPLC.Result:The speed and degree of dissolution of salidroside and catechin were improved in micro-powder than those in crude powder after a certain time（15-45 min,P < 0.05,45-60 min,P < 0.01）,but the difference of the gallic acid dissolution in the two kinds of powder were not obvious.Conclusion:Both the dissolution of effect component in R.rosea and the effect of Rhodiola rosea can be improved by SFCT.关键词：Rhodiola rosea;super-fine crushing technique;gallic acid;salidroside;catechin;dissolution
- 0
- |
- 184
- |
- 4
发布时间：2024-09-23 - 摘要：Objective: To investigate the experimental proof for the application of super fine crushing technique (SFCT) to the traditional Chinese medicinal herb and develop new methods to evaluate the effect of SFCT on drug. Method: The dissolution of Rhodiola rosea prepared by different techniques was investigated by dialysis determining gallic acid,salidroside and catechin as the test marker. These markers were determined at different time by RP-HPLC. Result: The speed and degree of dissolution of salidroside and catechin were improved in micro-powder than those in crude powder after a certain time (15- 45 min, P<0.05, 45- 60 min, P<0.01), but the difference of the gallic acid dissolution in the two kinds of powder were not obvious. Conclusion: Both the dissolution of effect component in R.rosea and the effect of Rhodiola rosea can be improved by SFCT.关键词：Rhodiola rosea;super-fine crushing technique;gallic acid;salidroside;catechin;dissolution
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To study the qualitative standard of Fuke suppository, so as to better control the quality of this product. Method: To establish the thin layer chromatography (TLC) for Rhizoma Coptidis, Cortex Phellodendri Chinensis, Radix Sophorae Flavescentis, Fructus Cnidii, and determination method for gentiopicrin by the high performance liquid chromatography (HPLC). Result : The TLC method could well identify Rhizoma Coptidis, Cortex Phellodendri Chinensis, Radix Sophorae Flavescentis, Fructus Cnidii, with clear spots, non-interference of negative controls and good specificity. Gentiopicrin was linear in the range of 0.688~6.880 μg, with a good correlation coefficient(r=0.999 9). The average recovery is 101.91%, RSD as 2.00%(n=6). Conclusion: This method is simple, reliable and precise, can be used for qualitative control of this preparation.关键词：Fuke suppository;TLC;gentiopicrin;HPLC
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the qualitative standard of Fuke suppository,so as to better control the qual-ity of this product.Method:To establish the thin layer chromatography（TLC） for Rhizoma Coptidis,Cortex Phel-lodendri Chinensis,Radix Sophorae Flavescentis,Fructus Cnidii,and determination method for gentiopicrin by the high performance liquid chromatography（HPLC）.Result:The TLC method could well identify Rhizoma Coptidis,Cortex Phellodendri Chinensis,Radix Sophorae Flavescentis,Fructus Cnidii,with clear spots,non-interference of negative controls and good specificity.Gentiopicrin was linear in the range of 0.688 ～ 6.880 μg,with a good corre-lation coefficient（r = 0.999 9）.The average recovery is 101.91%,RSD as 2.00%（n = 6）.Conclusion:This method is simple,reliable and precise,can be used for qualitative control of this preparation.关键词：Fuke suppository;TLC;gentiopicrin;HPLC
- 0
- |
- 277
- |
- 3
发布时间：2024-09-23 - 摘要：Objective:To establish the methods of determination on the contents of total bile acid in Bile Arisaema by ultraviolet spectrophometry.Method:To add 50% sulfuric acid to the sample and react in water bath at 75 ℃.The contents of total bile acid were determined by ultraviolet spectrophometry on（377 ± 2） nm,using chenodeoxycholic acid（CDCA） as the chemical reference substance.Result:The linear response range was 0.000 0 ～ 0.431 2 g.L-1,r = 0.999 1.The average recovery was 98.79%（n = 5）,RSD 3.02%.Conclusion:The method was accurate,simple and reproducible which could be used for the determination of total bile acid in Bile Arisaema.关键词：Bile Arisaema;chenodeoxycholic acid;total bile acid;determination;ultraviolet spectroph-ometry
- 0
- |
- 434
- |
- 19
发布时间：2024-09-23 - 摘要：Objective: To establish the methods of determination on the contents of total bile acid in Bile Arisaema by ultraviolet spectrophometry. Method: To add 50% sulfuric acid to the sample and react in water bath at 75 ℃. The contents of total bile acid were determined by ultraviolet spectrophometry on (377±2) nm, using chenodeoxycholic acid (CDCA) as the chemical reference substance. Result: The linear response range was 0.000 0~0.431 2 g ·L-1, r=0.999 1. The average recovery was 98.79%(n=5), RSD 3.02%. Conclusion: The method was accurate, simple and reproducible which could be used for the determination of total bile acid in Bile Arisaema.关键词：Bile Arisaema;chenodeoxycholic acid;total bile acid;determination;ultraviolet spectrophometry
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To establish the internal standard multi-control method and applied to the determina-tion of flavonoid compounds in rutaceae plant:citrus aurantium,bitter orange Immature,walnut peel and tangerine peel.Method:Naringin as index,establishing the relative correction factor of naringin,hesperidin and neohesperi-din and investigating the durability.Internal standard multi-control method was used to determine the content of fla-vonoid compounds in rutaceae plant,using included angle cosine method to compare the calculated values with measured values by external standard method.Result:The flow rate,column temperature,columns and instruments did not have significant effect on the relative correction factor and there was no significant difference between the calculated values of determination of flavonoid compounds in rutaceae plant by "internal standard multi-control method"and measured values by external standard method.Conclusion:The established internal standard multi-control method can be used to quantitative analysis and quality control of flavonoid compounds in citrus aurantium,bitter orange immature,walnut peel and tangerine peel.关键词：internal standard multi-control method;naringin;hesperidin;neohesperidin;content deter-minnation
- 0
- |
- 861
- |
- 29
发布时间：2024-09-23 - 摘要：Objective: To establish the internal standard multi-control method and applied to the determination of flavonoid compounds in rutaceae plant: citrus aurantium, bitter orange Immature, walnut peel and tangerine peel. Method: Naringin as index, establishing the relative correction factor of naringin, hesperidin and neohesperidin and investigating the durability. Internal standard multi-control method was used to determine the content of flavonoid compounds in rutaceae plant, using included angle cosine method to compare the calculated values with measured values by external standard method. Result: The flow rate, column temperature, columns and instruments did not have significant effect on the relative correction factor and there was no significant difference between the calculated values of determination of flavonoid compounds in rutaceae plant by "internal standard multi-control method" and measured values by external standard method. Conclusion: The established internal standard multi-control method can be used to quantitative analysis and quality control of flavonoid compounds in citrus aurantium, bitter orange immature, walnut peel and tangerine peel.关键词：internal standard multi-control method;naringin;hesperidin;neohesperidin;content determinnation
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:测定抗眩晕颗粒中泽泻醇B乙酸酯的含量。方法:以AGT C18色谱柱（4.6 mm×250 mm,5μm）为固定相,乙腈-水（85∶15）为流动相,检测波长208 nm。结果:线性范围为0.28～3.50 g.L-1,r=0.999 2,回收率为99.37%,RSD为1.38%。结论:该方法用于测定抗眩晕颗粒中泽泻醇B乙酸酯的含量具有操作简便、结果准确、灵敏的特点,可用于该制剂的质量控制。关键词：高效液相色谱法;泽泻醇B乙酸酯;抗眩晕颗粒
- 0
- |
- 68
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To develop an HPLC method for determination of the content of caffeic acid in PYJP. Method: An C18 column was used as the chromatographic column.The mobile phase consisted of methanol-phosphate buffer (23 ∶77),the flow rate was 1.0 mL·min-1.The UV detection wavelength was at 323 nm. Result: There was a good linear relationship between the concentration of caffeic acid and peak area value when the concentrations of caffeic acid was within the range of 0.072 96- 0.437 76 μg(r=0.999 9).The average recovery was 99.10%,RSD 1.22%(n=5). Conclusion: The method was accurate, sensitive,reproducible and may be used in the determination of caffeic acid in PYJP关键词：PYJP;caffeic acid;HPLC;determination
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To develop an HPLC method for determination of the content of caffeic acid in PYJP.Method:An C18 column was used as the chromatographic column.The mobile phase consisted of methanol-phosphate buffer（23∶ 77）,the flow rate was 1.0 mL.min-1.The UV detection wavelength was at 323 nm.Result:There was a good linear relationship between the concentration of caffeic acid and peak area value when the concentrations of caffeic acid was within the range of 0.072 96-0.437 76 μg（r = 0.999 9）.The average recovery was 99.10%,RSD 1.22%（n = 5）.Conclusion:The method was accurate,sensitive,reproducible and may be used in the determina-tion of caffeic acid in PYJP关键词：PYJP;caffeic acid;HPLC;determination
- 0
- |
- 53
- |
- 0
发布时间：2024-09-23 - 摘要：目的:气相色谱内外标法测定鱼腥草含量比较。方法:采用气相色谱测定鱼腥草中甲基正壬酮的含量,HP-5弹性石英毛细管柱。结果:甲基正壬酮在24～366 ng线性关系良好,r=0.999,平均回收率99.61%。结论:气相色谱外标法甲基正壬酮的测定方法稳定,重复性好,可作为鱼腥草药材质量控制方法。关键词：气相色谱法;外标法;内标法;甲基正壬酮
- 0
- |
- 221
- |
- 11
发布时间：2024-09-23 - 摘要：目的:建立麻辛止咳胶囊质量标准。方法:用薄层色谱法对处方中的辛夷、枳壳、黄芩、细辛进行定性鉴别;用高效液相色谱法对样品中的麻黄碱进行含量测定。结果:薄层鉴别的色谱斑点清晰,阴性对照无干扰;含量测定盐酸麻黄碱进样量在0.249～7.470μg呈良好的线性关系,平均回收率为97.50%（n=5）,RSD为0.86%。结论:定性、定量方法简便、专属、准确,可用于麻辛止咳胶囊的初步质量控制。关键词：麻黄碱;薄层色谱法;高效液相色谱法
- 0
- |
- 56
- |
- 6
发布时间：2024-09-23 - 摘要：目的:测定调胃舒肝丸中厚朴酚、和厚朴酚的含量。方法:HPLC法,Zorbax SB C18色谱柱（4.6 mm×150 mm,5μm）,流动相甲醇-0.1%磷酸溶液（62∶38）,检测波长为294 nm。结果:厚朴酚在0.145 9～0.437 8μg线性良好（r=0.999 9,n=5）,和厚朴酚在0.028 5～0.085 5μg线性良好（r=0.999 6,n=5）。加样回收率厚朴酚为101.73%,RSD 0.57%（n=6）、和厚朴酚为97.27%,RSD 0.82%（n=6）。结论:本试验处理方法简单,测定结果稳定可靠,可作为调胃舒肝丸中厚朴的含量测定方法。关键词：调胃舒肝丸;厚朴酚;和厚朴酚;高效液相色谱法
- 0
- |
- 119
- |
- 6
发布时间：2024-09-23 - 摘要：目的:研究补肺丸中黄芪甲苷的含量测定方法。方法:采用高效液相色谱-蒸发光散射（HPLC-ELSD）法,以Wa-ters SymmetryShieldTM C18色谱柱分析;流动相乙腈-水（36∶64）;流速1.0 mL.min-1;漂移管温度90℃;氮气压力25 psi。结果:黄芪甲苷在0.367 2～2.203 2μg呈良好的线性关系,r=0.999 8;平均回收率为95.49%,RSD为2.5%（n=6）。结论:该方法简便、快捷、灵敏、准确,可作为补肺丸的质量控制方法。关键词：补肺丸;黄芪甲苷;高效液相色谱-蒸发光散射
- 0
- |
- 96
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: To study the liposoluble components from the liquor of the Panax ginseng. Method: The liposoluble components of P. ginseng were separated by GC-MS and identified by means of indexing the spectrum storeroom. Result: Forty compounds were obtained and 18 of them were identified. Conclusion: This research provided theoretical evidences for the far-reaching use of P. ginseng.关键词：Panax ginseng;liposoluble component;GC-MS
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the liposoluble components from the liquor of the Panax ginseng.Method:The liposoluble components of P.ginseng were separated by GC-MS and identified by means of indexing the spec-trum storeroom.Result:Forty compounds were obtained and 18 of them were identified.Conclusion:This research provided theoretical evidences for the far-reaching use of P.ginseng.关键词：Panax ginseng;liposoluble component;GC-MS
- 0
- |
- 233
- |
- 15
发布时间：2024-09-23 - 摘要：Objective: To determinate the contents of total flavonoids in the prepared medical herbs of Cyperi Rhizoma from different areas. Method: The contents of total flavonoids were detected by UV while rutin was used as controller and measurement wavelength was 507 nm. Result: The results showed that rutin had a good Linear relationship in the concentrations between 3.0 mg·L-1 to 50.0 mg·L-1 (r=0.999 8). The contents of total flavonoids in most areas were 0.81％-1.75％ except from Shanghai which was 0.42%. Conclusion: The contents of total flavonoids of the prepared medical herbs of Cyperi Rhizoma were higher and it may be used as one of quality index.关键词：The prepared medical herbs of Cyperi Rhizoma;total flavonoids;UV;content
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To determinate the contents of total flavonoids in the prepared medical herbs of Cy-peri Rhizoma from different areas.Method:The contents of total flavonoids were detected by UV while rutin was used as controller and measurement wavelength was 507 nm.Result:The results showed that rutin had a good Line-ar relationship in the concentrations between 3.0 mg.L-1 to 50.0 mg.L-1（r = 0.999 8）.The contents of total flavonoids in most areas were 0.81%-1.75% except from Shanghai which was 0.42%.Conclusion:The contents of total flavonoids of the prepared medical herbs of Cyperi Rhizoma were higher and it may be used as one of quality index.关键词：The prepared medical herbs of Cyperi Rhizoma;total flavonoids;UV;content
- 0
- |
- 251
- |
- 11
发布时间：2024-09-23 - 摘要：Objective: The HPLC fingerprints of chemical composition were established to evaluate different collecting time and different growing areas of overground part of Desmodium styracifolium. Method: HPLC analysis was performed on a DiamonsilTM C18 column(4.6 mm×250 mm,5 μm) with the mobile phase in isocratic elution : ethanol(A)-H2O(0.2% phosphoric acid) (28 ∶72). The column temperature was setup at 40 ℃ and the flow-rate was 1.0 mL·min-1. The detection of wavelength was at 271 nm. Result: The D.styracifolium from Yangchun has distinct differences with other place of origin; The accumulation of flavones constituents showed a regular pattern in different collecting time. Conclusoin: The method is reliable and can be helpful on the quality control of D. styracifolium.关键词：Desmodium styracifolium;different collecting time;different growing area;HPLC fingerprint
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:The HPLC fingerprints of chemical composition were established to evaluate different collecting time and different growing areas of overground part of Desmodium styracifolium.Method:HPLC analysis was performed on a DiamonsilTM C18column（4.6 mm × 250 mm,5 μm） with the mobile phase in isocratic elution :ethanol（A）-H2O（0.2% phosphoric acid）（28∶ 72）.The column temperature was setup at 40 ℃ and the flow-rate was 1.0 mL.min-1.The detection of wavelength was at 271 nm.Result:The D.styracifolium from Yangchun has distinct differences with other place of origin;The accumulation of flavones constituents showed a regular pattern in different collecting time.Conclusoin:The method is reliable and can be helpful on the quality control of D.styraci-folium.关键词：Desmodium styracifolium;different collecting time;different growing area;HPLC fingerprint
- 0
- |
- 456
- |
- 19
发布时间：2024-09-23 - 摘要：Objective: To study the chemical constituents of Ludwigia adscendens. Method: The chemical constituents were repeatedly subjected to chromatography over Si gel and sephadex LH-20. Their structures were identified by spectroscopic experiments and chemical reactions. Result: Nine compounds were isolated from L. adscendens, and seven of them were established as hexadecanoic acid (Ⅰ), β-sitosterol (Ⅱ), bentulinic acid (Ⅲ), gallic acid (Ⅳ), ursolic acid (Ⅴ), quercetin-3-O-α-L-rhamnose (Ⅵ), and oleanolic acid (Ⅶ). Conclusion: Seven compounds were isolated from L. adscendens for the first time.关键词：Ludwigia adscendens;Ludwigia;chemical constituents
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents of Ludwigia adscendens.Method:The chemical constituents were repeatedly subjected to chromatography over Si gel and sephadex LH-20.Their structures were i-dentified by spectroscopic experiments and chemical reactions.Result:Nine compounds were isolated from L.ad-scendens,and seven of them were established as hexadecanoic acid（Ⅰ）,β-sitosterol（Ⅱ）,bentulinic acid（Ⅲ）,gallic acid（Ⅳ）,ursolic acid（Ⅴ）,quercetin-3-O-α-L-rhamnose（Ⅵ）,and oleanolic acid（Ⅶ）.Conclusion:Seven compounds were isolated from L.adscendens for the first time.关键词：Ludwigia adscendens;Ludwigia;chemical constituents
- 0
- |
- 288
- |
- 11
发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents from exocaep of Diospyros kaki.Method:Compounds were isolated by chromatographic techniques.The structures were identified by physicochemical properties and spectral analyses（1H-NMR,13 C-NMR,MS and IR）.Result:Seven compounds were isolated and identified as palmitic acid（1）,β-sitosterol（2）,ursolic acid（3）,24R-24-propylcholest-5-en-3β-ol（4）,oleanolic acid（5）,scopoletin（6）,myristic acid（7）.Conclusion:Compounds 4,6,7 were obtained from this plant for the first time.关键词：Diospyros kaki;chemical constituent;(24R)-24-propylcholest-5-en-3β-ol
- 0
- |
- 233
- |
- 15
发布时间：2024-09-23 - 摘要：Objective: To study the chemical constituents from exocaep of Diospyros kaki. Method: Compounds were isolated by chromatographic techniques. The structures were identified by physicochemical properties and spectral analyses(1H-NMR,13C-NMR,MS and IR). Result: Seven compounds were isolated and identified as palmitic acid(1),β-sitosterol(2),ursolic acid (3),24R-24-propylcholest-5-en-3β-ol(4),oleanolic acid(5),scopoletin(6),myristic acid(7). Conclusion: Compounds 4,6,7 were obtained from this plant for the first time.关键词：Diospyros kaki;chemical constituent;(24R)-24-propylcholest-5-en-3β-ol
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Helicobacter pylori on pGL3-Basic-COX-2-promoter transcriptional activity in gastric cancer MKN45 cells and the regulatory mechanism of Jianpi Jiedu Recipe on COX-2 promoter transcriptional activity.Method:The recombinant vector pGL3-Basic-COX-2-promoter was transient co-transfected into MKN45 cells,then detected the effect of H.pylori on COX-2 promoter activity.The effect of H.pylori on COX-2 promoter activity in human gastric cancer cells after blocking p38MAPK signal transduction pathway with a specific inhibitor SB203580 was observed.The effect of Jianpi Jiedu Recipe on H.pylori-stimulated phosphorylation of p38MAPK and ATF-2,the downstream transcription factor of p38MAPK,was investigated.Result:H.pylori could increase COX-2 promoter activity.COX-2 promoter activity down-regulated significantly afterblocking p38MAPK signal transduction pathway.Jianpi Jiedu Recipe inhibited H.pylori-induced p38MAPK and ATF-2 activity.Conclusion:H.pylori infection increases COX-2 promoter transcriptional activity via p38MAPK signal transduction pathway,and Jianpi Jiedu Recipe inhibits H.pylori-induced COX-2 promoter transcriptional activity through regulating p38MAPK/ATF-2 signal transduction pathway,which may be one of the mechanisms of prevention and treatment of gastric cancer.关键词：gastric cancer;helicobacter pylori;cyclooxygenase-2;promoter;Jianpi Jiedu Recipe;p38 mitogen-activated protein kinases;activating transcription factor-2
- 0
- |
- 221
- |
- 12
发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of Helicobacter pylori on pGL3-Basic-COX-2-promoter transcriptional activity in gastric cancer MKN45 cells and the regulatory mechanism of Jianpi Jiedu Recipe on COX-2 promoter transcriptional activity. Method: The recombinant vector pGL3-Basic-COX-2-promoter was transient co-transfected into MKN45 cells, then detected the effect of H. pylori on COX-2 promoter activity. The effect of H. pylori on COX-2 promoter activity in human gastric cancer cells after blocking p38MAPK signal transduction pathway with a specific inhibitor SB203580 was observed. The effect of Jianpi Jiedu Recipe on H. pylori-stimulated phosphorylation of p38MAPK and ATF-2, the downstream transcription factor of p38MAPK, was investigated. Result: H.pylori could increase COX-2 promoter activity. COX-2 promoter activity down-regulated significantly after blocking p38MAPK signal transduction pathway. Jianpi Jiedu Recipe inhibited H. pylori-induced p38MAPK and ATF-2 activity. Conclusion: H. pylori infection increases COX-2 promoter transcriptional activity via p38MAPK signal transduction pathway, and Jianpi Jiedu Recipe inhibits H. pylori-induced COX-2 promoter transcriptional activity through regulating p38MAPK/ATF-2 signal transduction pathway, which may be one of the mechanisms of prevention and treatment of gastric cancer.关键词：gastric cancer;helicobacter pylori;cyclooxygenase-2;promoter;Jianpi Jiedu Recipe;p38 mitogen-activated protein kinases;activating transcription factor- 2
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the action mechanism of Dihuang Yinzi on hypertension progress of spontaneously hypertensive rats（SHR）,and understand the role of endothelial function recovery by Dihuang Yinzi.Method:Fifty-four male SHR were divided into three groups:the control;Dihuang Yinzi treatment;and captoril treatment.Dihuang Yinzi（crude drug 15.9 g.kg-1.d-1） and captoril（3.375 g.kg-1.d-1） were treated from wk 7 to wk 24（wk = week） through gavage.The drug effect was observed at wk 18 and wk 24 during the treatments and 8 wk（wk32） after treatment.Experiments including the blood pressure by arterial carotis cannula;the concentration of NO 2-by Griess;the Ach-dependent relaxation of mesenteric rings by isometric tension change;and the mRNA expressions of IL-1,IL-6,TNF-α and iNOS of aorta tissue by real time PCR.Result:Dihuang Yinzisignificantly reduced blood pressure at wk18,and increased Ach-dependent relaxation of mesenteric rings and the NO 2-concentration in serum at wk18 and wk24.The mRNA expression of IL-1,IL-6,TNF-α and iNOS was markedly inhibited by the herb formula.Although captopril showed stronger anti-hypertensive effect,which could not extended after drug withdraw.In addition,the beta blocker did not enhance Ach-dependent vasodilation.NO 2-levels in serum and IL-1,IL-6 expression of aorta tissue were increased by captopril,while iNOS and TNF-α were inhibited at wk 24.Conclusion:Dihuang Yinzi showed anti-hypertensive effect with enhanced endothelium-dependent vasodilation in mesenteric artery and increased NO 2-concentration in serum.Furthermore,the herb formula also exerted anti-inflammatory effect by decreasing IL-1,IL-6,TNF-α and iNOS expression.关键词：endothelial dysfunction;inflammation;Dihuang Yinzi;captoril;spontaneously hypertensive rat（SHR）;real-time PCR
- 0
- |
- 260
- |
- 16
发布时间：2024-09-23 - 摘要：Objective: To investigate the action mechanism of Dihuang Yinzi on hypertension progress of spontaneously hypertensive rats (SHR), and understand the role of endothelial function recovery by Dihuang Yinzi. Method: Fifty- four male SHR were divided into three groups: the control; Dihuang Yinzi treatment; and captoril treatment. Dihuang Yinzi (crude drug 15.9 g ·kg-1 ·d-1) and captoril (3.375 g ·kg-1 ·d-1) were treated from wk 7 to wk 24 (wk=week) through gavage. The drug effect was observed at wk 18 and wk 24 during the treatments and 8 wk (wk32) after treatment. Experiments including the blood pressure by arterial carotis cannula; the concentration of NO-2by Griess; the Ach-dependent relaxation of mesenteric rings by isometric tension change; and the mRNA expressions of IL-1,IL-6,TNF-α and iNOS of aorta tissue by real time PCR. Result: Dihuang Yinzi significantly reduced blood pressure at wk18, and increased Ach-dependent relaxation of mesenteric rings and the NO-2 concentration in serum at wk18 and wk24. The mRNA expression of IL-1, IL-6, TNF-α and iNOS was markedly inhibited by the herb formula. Although captopril showed stronger anti-hypertensive effect, which could not extended after drug withdraw. In addition, the beta blocker did not enhance Ach-dependent vasodilation. NO-2levels in serum and IL-1, IL-6 expression of aorta tissue were increased by captopril, while iNOS and TNF-α were inhibited at wk 24. Conclusion: Dihuang Yinzi showed anti-hypertensive effect with enhanced endothelium-dependent vasodilation in mesenteric artery and increased NO-2concentration in serum. Furthermore, the herb formula also exerted anti-inflammatory effect by decreasing IL-1, IL-6, TNF-α and iNOS expression.关键词：endothelial dysfunction;inflammation;Dihuang Yinzi;captoril;spontaneously hypertensive rat (SHR);real-time PCR
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:实验观察甘草蜜炙前后补益作用的变化,为甘草炮制工艺研究提供药效学依据。方法:昆明种小鼠,按性别、体重均衡随机分组,每组10只,分组后连续给药10 d,观察小鼠碳粒廓清指数,剥离肝、脾和胸腺等脏器,计算脏器指数和吞噬活性a;连续10 d ig给予小鼠大黄水煎液20 g/kg,造成脾虚模型后给予相应受试药物10 d,观察小鼠的体重,一般状况的改善情况,爬杆时间,负重游泳试验等指标,解剖小鼠脾脏和胸腺,计算脏器指数。结果:各给药组均不同程度提高单核巨噬细胞的吞噬指数,其中水提取液中生品和炙品与空白组比较具有显著性差异,两种提取方式中除水提清炒甘草外,其余各组甘草均不同程度提高单核巨噬细胞的吞噬活性,其中炙甘草水提液较为明显。3个甘草炮制品的水提取液比较,炙甘草水提取液的胸腺指数最大,其次为生甘草水提取液。3个甘草炮制品的醇提取液比较,亦为炙甘草醇提取液指数最大,其次为生甘草醇提取液。3个甘草炮制品对胸腺指数的影响程度依次为炙甘草、生甘草、清炒甘草。各组爬杆时间比较,生甘草水提取液和2种甘草的醇提取液明显长于空白对照组。各受试药物组明显延长负重游泳时间,各药物组平衡比较,炙甘草两个提取液组优于生甘草提取液两个组,其中炙甘草醇提取液组与模型对照组比较有显著性差异（P<0.05）。各给药组间平衡比较可见炙甘草水提取液和醇提取液两指数提高均较生甘草水提取液和醇提取液高。结论:甘草炮制前后主治功能有所改变,甘草蜜炙后可增强补益功能,与传统中医药理论吻合,同时可见炙甘草醇提取液的药效学作用可能优于水提取液。关键词：甘草;炮制工艺;动物模型/脾虚症;药效学
- 0
- |
- 1621
- |
- 55
发布时间：2024-09-23 - 摘要：Objective:To observe the Effects of Suanzaoren Tang（SZRT） on glutamic acid（Glu）,γ-aminobutyric acid（GABA） and γ-aminobutyric acid subtype A rceptor（GABAAR） expression in the brain of the elderly insomnia model in rats.Method:Multiple factors model were established with subacute senescence induced by D-galactose,Yin and blood-deficiency induced by cyclophosphamide and hydrocortisonum,and sleep deprivation（SD） induced by a modified multiple platform method（MMPM）.The levels of amino acid neurotransmitters Glu and GABA were detected in cortex and hypothalamus for the rats by HPLC using immunohistochemical staining and semi-quantitative analysis of a retrovirus-polymerase chain reaction（RT-PCR）,subunit expression of GABAAR α1 and γ2 were detected in cortical and hippocampal parts of the model rats.After intervention of SZRT,thoseindicators were investigated too.Result:Compared with the environmental control group,content of Glu,GABA and the ratio of them in cortex and hypothalamus in the insomnia model rats showed a considerable elevatio,GABAARα1 and γ2 subunits expressed by immunochemical accumulated absorbance in cortex and hippocampus in the models were significantly higher（P < 0.01）,and GABAARα1,γ2 mRNA expression in cortex showed higher significantly（P < 0.01）.While these indicators tended to be in the normal range after the treatment of SZRT.Conclusion:Markedly up-regulated expression of GABAAR α1 and γ2 in cerebral cortex and hippocampus may be one of the pathological mechanisms for the insomnia model with sleep deprivation.SZRT can reduce the toxic effects of amino acids in brain,and downward adjust GABAAR α1 and γ2subunit expression in the brain.This may be one of the mechanisms for SZRT treatment of insomnia.关键词：sleep deprivation;model;Suanzaoren Tang（SZRT）;amino acid neurotransmitters;mechanism
- 0
- |
- 1337
- |
- 64
发布时间：2024-09-23 - 摘要：目的:观察干姜、高良姜及丁香各自3种不同提取物（挥发油、水提液、去挥发油水提液）对交感神经-肾上腺机能的影响,探讨这3种药物的挥发油与其药性、功效的相关性,及其以汤剂入药的合理性。方法:设空白对照组、模型对照Ⅰ组、模型对照Ⅱ组、阳性对照组、干姜（高良姜、丁香）3种不同提取物高、低剂量组,用寒凉药复制大鼠寒证模型,采用酶联免疫法测尿内17-羟皮质类固醇（17-OHCS）的含量,荧光分光光度法测尿内儿茶酚胺（CAs）类物质肾上腺素（A）、去甲肾上腺素（NE）、多巴胺（DA）的含量。结果:干姜挥发油与水提液高、低剂量组,高良姜挥发油与去挥发油水提液油高、低剂量组,丁香挥发油与水提液高、低剂量组、去挥发油水提液高剂量组能显著增加17-OHCS的排出量（P<0.05或P<0.01）。干姜水提液高、低剂量组,挥发油高剂量组,高良姜水提液高剂量组、去挥发油水提液高、低剂量组,丁香挥发油高、低剂量组、水提液与去挥发油水提液高剂量组能显著增加A的排出量（P<0.05或P<0.01）。干姜挥发油与水提液高、低剂量组,高良姜水提液与去挥发油水提液高、低剂量组,丁香挥发油与水提液高、低剂量组、去挥发油水提液高剂量组能显著增加NE的排出量（P<0.05或P<0.01）。干姜挥发油与水提液高剂量组,高良姜水提液高剂量组,丁香3种提取物高剂量组能显著增加DA的排出量（P<0.05或P<0.05）。结论:干姜的有效成分主要集中在挥发油和水提液部分,高良姜、丁香的有效成分在3种提取物中都有存在,3药以水煎剂入药具有科学依据。干姜所含的挥发油与温热药性、功效有密切的相关性。高良姜所含的挥发油与温热药性、功效的相关性不显著,丁香所含的挥发油与温热药性、功效有一定的相关性。关键词：干姜;高良姜;丁香;挥发油;水提液;去挥发油水提液;寒证;交感神经-肾上腺机能
- 0
- |
- 710
- |
- 29
发布时间：2024-09-23 - 摘要：目的:研究敏咳清颗粒含药血清对经肝素刺激增殖的人胚肺成纤维细胞（lung fibrobast,LFB）表达纤维连接蛋白（fibronectin,FN）、干细胞因子（stem cell factor,SCF）的影响,探讨敏咳清颗粒治疗咳嗽变异性哮喘的作用机制。方法:将常规培养LFB细胞分为空白组、模型组、对照组、敏咳清高剂量组、敏咳清低剂量组、养阴清肺糖浆高剂量组、养阴清肺糖浆低剂量组、地塞米松组,加入肝素刺激后分别加入对应的含药血清,采用ELISA法检测各组细胞培养上清液中FN,SCF的水平。结果:①各组含药血清均可不同程度的抑制SCF的产生,敏咳清颗粒作用最强,并呈剂量依赖性;②敏咳清颗粒能明显抑制FN的表达,高、低剂量组之间相比无显著意义。结论:敏咳清颗粒能显著降低经肝素刺激增殖的LFB对FN,SCF的表达,提示抑制SCF,FN的表达,可能是其治疗咳嗽变异性哮喘（CVA）的作用机制之一。关键词：敏咳清颗粒;人胚肺成纤维细胞;纤维连接蛋白;干细胞因子
- 0
- |
- 110
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the protective effect of aqueous extract of Erzhi Pill（AEEP） on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.Method:Sixty Kunming mice were randomly divided into six groups:the normal group,the model group,bifendate group（150 mg.kg-1）,high AEEP group（19.8 g.kg-1）,middle AEEP group（13.2 g.kg-1） and low AEEP group（6.6 g.kg-1）.The treatment groups were orally administered once per day for 7 d separately,whereas the normal and model groups were orally administered with saline.Except normal rats,all the other rats were injected intraperitoneally CCl4 20 mL.kg-1 once.The rats were sacrificed 16 h after CCl4administration.Serum and liver samples were collected for analysis.The acute hepatic injury model was prepared by CCl4 injected intraperitoneally.Then,the therapeutic effects of AEEP on the model were evaluated by the activity determination of serum alanine aminotransferase and asparate aminotransferase（ALT and AST）,superoxide dismutase（SOD） and the content of malondialdehyde（MDA） in liver,and the hepatic pathohistological changes following the treatment.Result:The activities of ALT and AST and the MDA content in liver was significantly increased and the activity of SOD was largely inhibited in the animals ofmodeling group.Following the treatment with AEEP,ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group.Furthermore,AEEP could ameliorate the hepatic pathological changes.Conclusion:AEEP have protective effects on acute hepatic injury induced by CCl4in mice.关键词：Erzhi Pill;carbon tetrachloride;acute hepatic injury
- 0
- |
- 208
- |
- 16
发布时间：2024-09-23 - 摘要：Objective: To study the protective effect of aqueous extract of Erzhi Pill (AEEP) on acute hepatic injury induced by carbon tetrachloride(CCl4) in mice. Method: Sixty Kunming mice were randomly divided into six groups: the normal group, the model group, bifendate group(150 mg ·kg-1), high AEEP group(19.8 g ·kg-1), middle AEEP group(13.2 g ·kg-1) and low AEEP group(6.6 g ·kg-1). The treatment groups were orally administered once per day for 7 d separately, whereas the normal and model groups were orally administered with saline. Except normal rats, all the other rats were injected intraperitoneally CCl4 20 mL ·kg-1 once. The rats were sacrificed 16 h after CCl4administration. Serum and liver samples were collected for analysis. The acute hepatic injury model was prepared by CCl4injected intraperitoneally. Then, the therapeutic effects of AEEP on the model were evaluated by the activity determination of serum alanine aminotransferase and asparate aminotransferase (ALT and AST), superoxide dismutase(SOD)and the content of malondialdehyde (MDA)in liver, and the hepatic pathohistological changes following the treatment. Result: The activities of ALT and AST and the MDA content in liver was significantly increased and the activity of SOD was largely inhibited in the animals of modeling group. Following the treatment with AEEP, ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group. Furthermore, AEEP could ameliorate the hepatic pathological changes. Conclusion: AEEP have protective effects on acute hepatic injury induced by CCl4in mice.关键词：Erzhi Pill;carbon tetrachloride;acute hepatic injury
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To select a simple, stable and satisfied experimental model of renal calcium oxalate calculus in rats. Method: After several renal calcium oxalate calculus models currently used (Model group I: administered intragastrically with 1% ethylene glycol free drink+2% ammonium chloride 2 mL/rat per day; model Group Ⅱ: administered intragastrically with 1.25% ethylene glycol+1% ammonium chloride 20 mL ·kg-1 per day; model group Ⅲ: drank 10 mL 1.25% ethylene glycol+1% ammonium chloride and 30 mL water per day; model group Ⅳ: drank 1% ethylene glycol+1% ammonium chloride freely.) experimental rat models were developed. Several biochemical indicators of urine, blood and nephridial tissue were measured and renal pathological specimens were observed. Then the modeling results were comprehensive evaluated. Result: The 24 h urinary BUN, Cr, UA, IP, Ca2+, OX of model groupⅠwere singnificantly higher than the normal control group (groupⅤ) (P<0.01); blood BUN, Cr, UA, IP, Ca2+ were significantly higher than the normal control group (P<0.01); nephridial tissue Ca2+ and OX were significantly higher than the normal control group (P<0.01); and calcium oxalate crystals were distributed in nephridial tissue. The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats renal function breakdown, and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadnt been seen in nephridial tissue. All rats of model group Ⅳ were dead. Conclusion: Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.关键词：calcium oxalate;renal calculus;animal model;rat
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To select a simple,stable and satisfied experimental model of renal calcium oxalate calculus in rats.Method:After several renal calcium oxalate calculus models currently used（Model group I:administered intragastrically with 1% ethylene glycol free drink + 2% ammonium chloride 2 mL/rat per day;model Group Ⅱ:administered intragastrically with 1.25% ethylene glycol + 1% ammonium chloride 20 mL.kg-1 per day;model group Ⅲ:drank 10 mL 1.25% ethylene glycol + 1% ammonium chloride and 30 mL water per day;model group Ⅳ:drank 1% ethylene glycol + 1% ammonium chloride freely.） experimental rat models were developed.Several biochemical indicators[urea nitrogen（BUN）,creatinine（Cr）,uric acid（UA）,inorganic phosphorus（IP）,calcium（Ca2 +）,oxalate（OX）] of urine,blood and nephridial tissue were measured and renal pathological specimens were observed.Then the modeling results were comprehensive evaluated.Result:The 24 h urinary BUN,Cr,UA,IP,Ca2 +,OX of model groupⅠwere singnificantly higher than the normal control group（groupⅤ）（P < 0.01）;blood BUN,Cr,UA,IP,Ca2 + were significantly higher than the normal control group（P < 0.01）;nephridial tissue Ca2 + and OX were significantly higher than the normal control group（P < 0.01）;and calcium oxalate crystals were distributed in nephridial tissue.The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats’ renal function breakdown,and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadn’t been seen in nephridial tissue.All rats of model group Ⅳ were dead.Conclusion:Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.关键词：calcium oxalate;renal calculus;animal model;rat
- 0
- |
- 337
- |
- 22
发布时间：2024-09-23 - 摘要：Objective:To observe the anti-inflammatory,immune regulating effect of Tiezhou Qingzhuo Pill（TZQZP） and its effects on prostatic hyperplasia.Method:The hyperplasia of prostate induced by testosterone in castrated rat was investigated.Anti-inflammatory was evaluated by paw edema test and cotton bailing granulation in rats,and ear tumefaction in mice;immune regulating effect was observed by the means of carbon particle clearance and haemolysin antibodies.Result:TZQZP could obviously inhibit the index of prostate in prostatitis rat（P < 0.05）;inhibit the paw edema and cotton bailing granulation in rats,and ear tumefaction in mice（P < 0.05,P < 0.01）;enhance the phagocytic activity of monocytes and increased content of haemolysin antibody（P < 0.05）.Conclusion:TZQZP has evident effects of anti-inflammation,immune regulation,and inhibition of prostatic hyperplasia.关键词：Tiezhou Qingzhuo Pill;prostatic hyperplasia;anti-inflammation;immunity
- 0
- |
- 112
- |
- 4
发布时间：2024-09-23 - 摘要：Objective: To observe the anti-inflammatory,immune regulating effect of Tiezhou Qingzhuo Pill(TZQZP) and its effects on prostatic hyperplasia. Method: The hyperplasia of prostate induced by testosterone in castrated rat was investigated. Anti-inflammatory was evaluated by paw edema test and cotton bailing granulation in rats, and ear tumefaction in mice; immune regulating effect was observed by the means of carbon particle clearance and haemolysin antibodies. Result: TZQZP could obviously inhibit the index of prostate in prostatitis rat(P＜0.05); inhibit the paw edema and cotton bailing granulation in rats, and ear tumefaction in mice(P＜0.05, P＜0.01); enhance the phagocytic activity of monocytes and increased content of haemolysin antibody(P＜0.05). Conclusion: TZQZP has evident effects of anti-inflammation, immune regulation, and inhibition of prostatic hyperplasia.关键词：Tiezhou Qingzhuo Pill;prostatic hyperplasia;anti-inflammation;immunity
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: To observe and verify the intervention action of Sanhuang Yishen Capsule in rats with early diabetic nephropathy, and to study its possible mechanism, so as to provide experimental evidence for the clinic prevention of diabetic nephropathy. Method: Diabetic rats(n=13/group) were randomly divided into diabetes model group, low-dose Sanhuang Yishen Capsule treated group(0.4 g ·kg-1 ·d-1), middle-dose Sanhuang Yishen Capsule treated group(0.8 g ·kg-1 ·d-1), high-dose Sanhuang Yishen Capsule treated group(1.6 g ·kg-1 ·d-1). Ten SD rats were selected as control group. At the end of the 8th week, we detected blood glucose, glycosylated hemoglobin, creatinine, urea nitrogen, triglyceride, cholesterol and kidney weight/body weight index. The expression of renal TGF-β1 and VEGF were assessed by immune-histochemistry. Result: After treatment of 8 weeks,compared with the diabetes model group the middle-dose and the high-dose Sanhuang Yishen Capsule groups showed obviously decreases in kidney weight/body weight index and the level of serum creatinine, urea nitrogen (P<0.01). The expression of TGF-β1 and VEGF in kidney of middle-, high-dose Sanhuang Yishen Capsule treated group rats was lower than that of the diabetes model group(P<0.05). Conclusion: Sanhuang Yishen Capsule can reduce the expression of TGF-β1 and VEGF in the kidney of diabetic rats and delay of renal injury in diabetic rats.关键词：Sanhuang Yishen Capsule;diabetic nephropathy;transforming growth factor-β1(TGF-β1);vascular endothelial growth factor (VEGF)
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To observe and verify the intervention action of Sanhuang Yishen Capsule in rats with early diabetic nephropathy,and to study its possible mechanism,so as to provide experimental evidence for the clinic prevention of diabetic nephropathy.Method:Diabetic rats（n = 13/group） were randomly divided into diabetes model group,low-dose Sanhuang Yishen Capsule treated group（0.4 g.kg-1.d-1）,middle-dose Sanhuang Yishen Capsule treated group（0.8 g.kg-1.d-1）,high-dose Sanhuang Yishen Capsule treated group（1.6 g.kg-1.d-1）.Ten SD rats were selected as control group.At the end of the 8th week,we detected blood glucose,glycosylated hemoglobin,creatinine,urea nitrogen,triglyceride,cholesterol and kidney weight/body weight index.The expression of renal TGF-β1 and VEGF were assessed by immune-histochemistry.Result:After treatment of 8 weeks,compared with the diabetes model group the middle-dose and the high-dose Sanhuang Yishen Capsule groups showed obviously decreases in kidney weight/body weight index and the level of serum creatinine,urea nitrogen（P < 0.01）.The expression of TGF-β1 and VEGF in kidney of middle-,high-dose Sanhuang Yishen Capsule treated group rats was lower than that of the diabetes model group（P < 0.05）.Conclusion:Sanhuang Yishen Capsule can reduce the expression of TGF-β1 and VEGF in the kidney of diabetic rats and delay of renal injury in diabetic rats.关键词：Sanhuang Yishen Capsule;diabetic nephropathy;transforming growth factor-β1（TGF-β1）;vascular endothelial growth factor（VEGF）
- 0
- |
- 186
- |
- 41
发布时间：2024-09-23 - 摘要：Objective: To investigate the protective effect of the ethanol extract of Ligularia fischeri Ledeb Turcz. in mice on acute alcohol intoxication. Method: We recorded the duration from disappearance of righting reflex (drunk) to restore (hangover) in mice, after given a gavage of 56 degrees liquor. In addition, alcohol-induced liver injury model of mice was established by acute gavage of 52 degrees liquor, and serum alanine aminotransferase (ALT) activity, malondialdehyde (MDA), triglyceride (TG) content were tested. Activity of glutamic-pyruvic transaminase (ALT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), triglyceride (TG) in liver tissue was also determined. Result: The ethanol extract of L. fischeri significantly prolonged the incubation period of righting reflex disappeared (drunk) (P<0.05), reduced hangover time (P<0.05); significantly depressed TG and ALT levels (P<0.05) in serum and liver tissue; and significantly increased GSH-Px activity (P<0.01) in liver tissue. Conclusion: L. fischeri may have a protective effect on acute alcohol intoxication in mice.关键词：Ligularia fischeri;alcoholism;mice;triglyceride;alanine aminotransferase;glutathione peroxidase;malondialdehyde
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the protective effect of the ethanol extract of Ligularia fischeri Ledeb Turcz.in mice on acute alcohol intoxication.Method:We recorded the duration from disappearance of righting reflex（drunk） to restore（hangover） in mice,after given a gavage of 56 degrees liquor.In addition,alcohol-induced liver injury model of mice was established by acute gavage of 52 degrees liquor,and serum alanine aminotransferase（ALT） activity,malondialdehyde（MDA）,triglyceride（TG） content were tested.Activity of glutamic-pyruvic transaminase（ALT）,glutathione peroxidase（GSH-Px）,malondialdehyde（MDA）,triglyceride（TG） in liver tissue was also determined.Result:The ethanol extract of L.fischeri significantly prolonged the incubation period of righting reflex disappeared（drunk）（P < 0.05）,reduced hangover time（P < 0.05）;significantly depressed TG and ALT levels（P < 0.05） in serum and liver tissue;and significantly increased GSH-Px activity（P < 0.01） in liver tissue.Conclusion:L.fischeri may have a protective effect on acute alcohol intoxication in mice.关键词：Ligularia fischeri;alcoholism;mice;triglyceride;alanine aminotransferase;glutathione peroxidase;malondialdehyde
- 0
- |
- 141
- |
- 7
发布时间：2024-09-23 - 摘要：Objective: To study the effect of Yangyuan Yin on decreasing alcohol in blood and protecting liver after drinking alcohol. Method: The model of alcoholic liver injury in rats was established by giving alcohol ig for 6 weeks. Meanwhile, Yangyuan Yin was given to the rats in the treatment group. By the end of the 6th week, alcohol content in blood was determined by GC, colorimetry was applied to determine ADH activity in liver and ALT, AST in blood serum. Result: Middle and high doses of Yangyuan Yin could significantly reduce the levels of alcohol and acetaldehyde in blood. Actually, the high dose could significantly improve the level of liver ADH, better than that of Haiwangjinzun. Actually, compared to the model, the activity of ALT and AST was decreased by the treatment. Conclusion: Yangyuan Yin is able to decrease alcohol in blood after drinking alcohol. The effects may be achieved by increasing the level of liver ADH.关键词：Yangyuan Yin;anti-alcoholism;liver protection;alcohol dehydrogenase;alanine aminotransferase;aspartate aminotransferase
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To study the effect of Yangyuan Yin on decreasing alcohol in blood and protecting liver after drinking alcohol.Method:The model of alcoholic liver injury in rats was established by giving alcohol ig for 6 weeks.Meanwhile,Yangyuan Yin was given to the rats in the treatment group.By the end of the 6th week,alcohol content in blood was determined by GC,colorimetry was applied to determine ADH activity in liver and ALT,AST in blood serum.Result:Middle and high doses of Yangyuan Yin could significantly reduce the levels of alcohol and acetaldehyde in blood.Actually,the high dose could significantly improve the level of liver ADH,better than that of Haiwangjinzun.Actually,compared to the model,the activity of ALT and AST was decreased by the treatment.Conclusion:Yangyuan Yin is able to decrease alcohol in blood after drinking alcohol.The effects may be achieved by increasing the level of liver ADH.关键词：Yangyuan Yin;anti-alcoholism;liver protection;alcohol dehydrogenase;alanine aminotransferase;aspartate aminotransferase
- 0
- |
- 375
- |
- 16
发布时间：2024-09-23 - 摘要：Objective: To investigate the effects of Danshen Chuanxinlian Decoction(DCD) on the hemorheological parameters of the rat model with high blood viscosity. Method: Fifty rats were divided into 5 groups: control group,model group and experimental groups. After 7 days of medication, a model with high blood viscosity by injecting NE and iced bath, and observed the effects of these two herbs on hemocheological parameters. Result: Blood viscosity and the content of plasma fibrinogen were decreased in DCD group compared with those in model group, and the combination of Danshen and Chuanxinlian could inhibit platelet aggregation and prolong prothrombin time. Conclusion: The combination of Danshen and Chuanxinlian can obviously improve the hemorheological parameters in rats with high blood viscosity.关键词：Danshen Chuanxinlian Decoction;blood viscosity;hemorheology
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of Danshen Chuanxinlian Decoction（DCD） on the hemorheological parameters of the rat model with high blood viscosity.Method:Fifty rats were divided into 5 groups:control group,model group and experimental groups.After 7 days of medication,a model with high blood viscosity by injecting NE and iced bath,and observed the effects of these two herbs on hemocheological parameters.Result:Blood viscosity and the content of plasma fibrinogen were decreased in DCD group compared with those in model group,and the combination of Danshen and Chuanxinlian could inhibit platelet aggregation and prolong prothrombin time.Conclusion:The combination of Danshen and Chuanxinlian can obviously improve the hemorheological parameters in rats with high blood viscosity.关键词：Danshen Chuanxinlian Decoction;blood viscosity;hemorheology
- 0
- |
- 83
- |
- 4
发布时间：2024-09-23 - 摘要：Objective: To discuss the molecular mechanism of Ease Powder decoction treating on sleep deprivation depression(SDD) by observing the changes of autonomic-behavior and the expression of hippocampus 5-HT1A receptor and 5-HT2A receptor in a rat experimental model of sleep deprivation depression. Method: 48 Wistar rats were divided into four groups after first open-field testing(OFT), they were normal group, model group, mirtazapine group and Ease Powder decoction group. Except the normal group, the other groups were received different stress for 21 days, then made 72 h rapid eye movement (REM), taken the drug every day before the stress(1 h). Meanwhile, the model group, mirtazapine group and Ease Powder decoction group were intragastric administration of normal saline,mirtazapine,Ease Powder decoction respectively. At the end of 21 days, all the animals were sacrificed. The hippocampus of rats were taken then kept in liquid nitrogen. Result: Compared with the situation before stress, there is a significant decrease in the autonomic-behavior of SDD after 21 days(P<0.01). Compared with the model group, the rats autonomic-behavior in Ease Powder decoction group and the mirtazapine group is increased. Compared with the normal group, the expression of 5-HT1AR mRNA in hippocampus of model group is decreased, the expression of 5-HT2AR mRNA is increased,with statistical significance(P<0.05). Comparison for expression of hippocampus 5-HT1AR mRNA and 5-HT2AR mRNA between the Ease Powder decoction group and the model group is significant(P<0.01). However, compared with the model group, the mirtazapine group has no statistical significance. Comparison of Ease Powder decoction group and mirtazapine group has statistical significance (P<0.05). Conclusion: The Ease Powder decoction exert an effect on SDD by regulating the expression of hippocampus 5-HT1AR and 5-HT2AR.关键词：sleep deprivation depression;5-hydroxytryptamine;Ease Powder decoction
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To discuss the molecular mechanism of Ease Powder decoction treating on sleep deprivation depression（SDD） by observing the changes of autonomic-behavior and the expression of hippocampus 5-HT1A receptor and 5-HT2A receptor in a rat experimental model of sleep deprivation depression.Method:48 Wistar rats were divided into four groups after first open-field testing（OFT）,they were normal group,model group,mirtazapine group and Ease Powder decoction group.Except the normal group,the other groups were received different stress for 21 days,then made 72 h rapid eye movement（REM）,taken the drug every day before the stress（1 h）.Meanwhile,the model group,mirtazapine group and Ease Powder decoction group were intragastric administration of normal saline,mirtazapine,Ease Powder decoction respectively.At the end of 21 days,all the animals were sacrificed.The hippocampus of rats were taken then kept in liquid nitrogen.Result:Compared with the situation before stress,there is a significant decrease in the autonomic-behavior of SDD after 21 days（P <0.01）.Compared with the model group,the rat’s autonomic-behavior in Ease Powder decoction group and the mirtazapine group is increased.Compared with the normal group,the expression of 5-HT1AR mRNA in hippocampus of model group is decreased,the expression of 5-HT2A R mRNA is increased,with statistical significance（P < 0.05）.Comparison for expression of hippocampus 5-HT1AR mRNA and 5-HT2AR mRNA between the Ease Powder decoction group and the model group is significant（P < 0.01）.However,compared with the model group,the mirtazapine group has no statistical significance.Comparison of Ease Powder decoction group and mirtazapine group has statistical significance（P < 0.05）.Conclusion:The Ease Powder decoction exert an effect on SDD by regulating the expression of hippocampus 5-HT1AR and 5-HT2AR.关键词：sleep deprivation depression;5-hydroxytryptamine;Ease Powder decoction
- 0
- |
- 665
- |
- 32
发布时间：2024-09-23 - 摘要：Objective:To investigate the influence of different Chinese medicine treatments on NF-кB signal transduction pathway of myocardial ischemia-reperfusion（I /R） injury in rats.Method:Sixty SD rats were divided into 5 groups randomly,sham-operated group:model group,removing phlegm and enlarging chest group,activating blood and dissolving stasis group,treating both phlegm and blood stasis group.The model of I /R of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.NIK,IKKβ and IкBα protein expression of myocardial tissues in rats were detected by Western blotting method.And the levels of NF-кBp65 mRNA were determined with RT-PCR.Result:Compared with model group,the expression of NIK,IKKβ and NF-кBp65 mRNA were lower,and the protein expression of IкBα was increased in all groups treated by different Chinese medicine treatments（P < 0.01）.Conclusion:The removing phlegm andenlarging chest method,activating blood and dissolving stasis method,treating both phlegm and blood stasis method can protect myocardium from I /R.The mechanism of action was related to inhibiting the protein expression of NIK,IKKβ and enhancing the expression of IкBα,restraining the activation of NF-кB.关键词：different Chinese medicine treatments;myocardial ischemia-reperfusion injury;signal transduction pathway
- 0
- |
- 177
- |
- 9
发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of different Chinese medicine treatments on NF-кB signal transduction pathway of myocardial ischemia-reperfusion(I/R) injury in rats. Method: Sixty SD rats were divided into 5 groups randomly, sham-operated group: model group, removing phlegm and enlarging chest group, activating blood and dissolving stasis group, treating both phlegm and blood stasis group. The model of I/R of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK, IKKβ and IкBα protein expression of myocardial tissues in rats were detected by Western blotting method. And the levels of NF-кBp65 mRNA were determined with RT-PCR. Result: Compared with model group, the expression of NIK,IKKβ and NF-кBp65 mRNA were lower,and the protein expression of IкBα was increased in all groups treated by different Chinese medicine treatments(P<0.01). Conclusion: The removing phlegm and enlarging chest method, activating blood and dissolving stasis method, treating both phlegm and blood stasis method can protect myocardium from I/R. The mechanism of action was related to inhibiting the protein expression of NIK, IKKβ and enhancing the expression of IкBα, restraining the activation of NF-кB.关键词：different Chinese medicine treatments;myocardial ischemia-reperfusion injury;signal transduction pathway
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:观察双花解毒颗粒对豚鼠变应性接触性皮炎的抑制作用。方法:10%二硝基氯苯（DNCB）-丙酮溶液涂抹去毛皮肤复制豚鼠变应性接触性皮炎模型,口服给药,通过观察耳廓肿胀度和病理图片,观察药物各剂量组的作用。结果:双花解毒颗粒5个剂量组均显著减小豚鼠耳肿胀度,但无明显量效关系。结论:双花解毒颗粒对豚鼠变应性接触性皮炎有明显抑制作用。关键词：双花解毒颗粒;变应性接触性皮炎;豚鼠
- 0
- |
- 128
- |
- 2
发布时间：2024-09-23 - 摘要：目的:研究清热解毒剂的急性毒性、镇痛、抗炎作用。方法:采用最大耐受量（MTD）法研究其急性毒性,通过热板法和扭体法观察清热解毒剂的镇痛作用。采用二甲苯致小鼠耳肿胀、冰醋酸致小鼠毛细血管通透性增高、棉球致肉芽肿等方法观察该药的抗炎作用。结果:清热解毒剂MTD相当于人临床日用量的222倍（人每日用药量为0.9 g.kg-1）;清热解毒剂中剂量对小鼠醋酸所致扭体反应有明显的镇痛作用;各剂量对对热板法引起的疼痛有不同程度的镇痛作用,其中中剂量镇痛作用的持续时间最长,药后120 min仍有明显的镇痛作用（P<0.05）;中剂量对二甲苯致小鼠耳廓肿胀及醋酸致小鼠腹腔毛细血管通透性的增高有明显的抑制作用（P<0.05）;各剂量对棉球致小鼠肉芽肿没有抑制作用。结论:清热解毒剂毒性较低,具有一定的镇痛作用,对急性炎症有抑制作用。关键词：清热解毒剂;镇痛;抗炎
- 0
- |
- 114
- |
- 6
发布时间：2024-09-23 - 摘要：目的:研究左归丸对老年性痴呆（AD）模型大鼠海马与皮质神经细胞凋亡的影响。方法:以慢性铝中毒法复制AD大鼠模型,随机分为模型组、西药组、左归丸低、中、高剂量组,另设空白对照组;用TUNEL法观测大鼠海马和皮质内细胞凋亡水平,光镜下计数阳性表达细胞。结果:模型组大鼠海马和皮质具有典型的细胞凋亡特征,而左归丸组的海马和皮质神经细胞凋亡数减少,以中、高剂量组作用较强。与模型组比较,有显著性差异（P<0.05,P<0.01）。结论:左归丸具有抑制AD模型大鼠海马皮质与神经细胞凋亡的作用。关键词：左归丸;老年性痴呆;海马神经细胞;皮质神经细胞
- 0
- |
- 235
- |
- 13
发布时间：2024-09-23 - 摘要：目的:研究制何首乌、大黄酚对小鼠肝脏细胞凋亡的影响。方法:制何首乌、大黄酚按一定剂量ig小鼠,1次/d,连续4周,检测转氨酶,用AnnexinV-FITC细胞凋亡检测试剂盒给予凋亡细胞染色,利用流式细胞仪检测细胞凋亡的情况。结果和结论:制何首乌组、大黄酚组均能提高小鼠肝细胞凋亡率,与空白对照组比较有显著差异。关键词：制何首乌;大黄酚;小鼠;细胞凋亡
- 0
- |
- 359
- |
- 15
发布时间：2024-09-23 - 摘要：目的:研究抑郁平胶囊的抗抑郁作用,并对其有效部位进行筛选。方法:采用小鼠悬尾和小鼠强迫游泳实验的2种行为绝望法复制小鼠抑郁模型,对抑郁平胶囊进行抗抑郁作用及筛选有效部位的研究。结果:抑郁平胶囊可明显缩短小鼠强迫游泳不动时间、悬尾不动时间（P<0.05或P<0.01）。结论:抑郁平胶囊有明显的抗抑郁作用,并且采用乙醇提取60%乙醇洗脱的样品较其他提取精制方法有更加明显抗抑郁作用。关键词：抑郁平胶囊;小鼠强迫游泳试验;小鼠悬尾试验
- 0
- |
- 115
- |
- 8
发布时间：2024-09-23 - 摘要：Objective:To observe the effect of the bioconversion product of jade screen powder on cellular immunity in immunosuppressed mice.Method:The levels of CD4 + T,CD8 + T,CD4 + T/CD8 + T were determined by flow cytometry;The content of serum interleukin-2（IL-2） and interferon-γ（IFN-γ） were detected by ELISA.Result:Compared with cyclophosphamide group,the levels of CD4 + T,CD8 + T,CD4 + T/CD8 + T improved in experimental group（P < 0.01 or P < 0.05） and the content of serum IL-2 and IFN-γ improved obviously in experimental group（P < 0.01）.Conclusion:The bioconversion product of Jade Screen Powder can promote the cell immunity function,through regulating the balance between CD4 + T and CD8 + T cells and correcting Th1/Th2 drift phenomenon to keep the superiority of Th1.关键词：Jade Screen Powder;biotransformation;cellular immunity
- 0
- |
- 174
- |
- 9
发布时间：2024-09-23 - 摘要：Objective: To observe the effect of the bioconversion product of jade screen powder on cellular immunity in immunosuppressed mice. Method: The levels of CD4+T,CD8+T,CD4+T/CD8+T were determined by flow cytometry;The content of serum interleukin-2(IL-2) and interferon-γ(IFN-γ) were detected by ELISA. Result : Compared with cyclophosphamide group,the levels of CD4+T,CD8+T,CD4+T/CD8+T improved in experimental group(P<0.01 or P<0.05) and the content of serum IL-2 and IFN-γ improved obviously in experimental group(P<0.01). Conclusion : The bioconversion product of Jade Screen Powder can promote the cell immunity function, through regulating the balance between CD4+T and CD8+T cells and correcting Th1/Th2 drift phenomenon to keep the superiority of Th1.关键词：Jade Screen Powder;biotransformation;cellular immunity
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:确认药典常规法是否适合于同芬和培欣的微生物限度检查,并取得适宜的检查方法。方法:按2005年版《中国药典》微生物限度检查法及方法学验证要求,对同芬和培欣进行了方法学验证。结果:同芬和培欣对大肠埃希菌、金黄色葡萄球菌和枯草芽孢杆菌抑菌作用强,培养基稀释法阳性对照菌回收率仍低于70%。结论:对同芬和培欣进行微生物限度检查,应按照2005年版《中国药典》要求,通过方法验证实验建立相应的合理的检验方法。关键词：方法学验证;微生物限度;同芬;培欣
- 0
- |
- 20
- |
- 0
发布时间：2024-09-23 - 摘要：Objective: In order to search potent intervening means for low-level exercise-associated cellular immune capacities, effect of Radix Astragali injection and Aerobic exercise on quantities of peripheral T lymphocyte subsets in rats was observed. Method: 50 Wister Rats were randomLy divided into 5 group: quiet control group(n=10), once exhausting exercise group(once exhausting exercise before drawning materials)(n=10), Aerobic exercise and once exhausting exercise group(once exhausting exercise after aerobic exercise for four weeks)(n=10), Radix Astragali injection and once exhausting exercise group(once exhausting exercise after oraling Radix Astragali injection for four weeks)(n=10), Radix Astragali injection and Aerobic exercise and once exhausting exercise group(once exhausting exercise after oraling Radix Astragali injection and for aerobic exercise four weeks)(n=10). After 4 weeks test, time of exhaustive exercise were record,percentage of peripheral T Lymphocyte subsets in circulating blood were tested by FCM,and body weight were measured before and after experiment. Result: Afer exhausting exercise, percentage of CD4+ and the ratio of CD4+ to CD8+ in Lymphocyte subsets,as well as were lower(P<0.01),percentage of CD8+ as well as were higher than that in normal control group(P<0.01).The change was prevented by Radix Astragali injection and aerobic exercise; the exhausting exercise time of aerobic exercise and once exhausting exercise group,astragali injection and once exhausting exercise group,Radix Astragali injection and aerobic exercise and once exhausting exercise group as well as were longer than once exhausting exercise group(P<0.01),and the exhausting exercise time of Radix Astragali injection and aerobic exercise and once exhausting exercise group as well as were longer than aerobic exercise and once exhausting exercise group,Astragali injection and once exhausting exercise group(P<0.01). Conclusion: Radix Astragali injection and aerobic exercise may improve exercise capacity and preven immunosuppression in rats after exhaustive exercise in rats, enhance effect after combinating the two means.关键词：Radix Astragali injection;exhausting exercise;aerobic exercise;CD4+/CD8+
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:In order to search potent intervening means for low-level exercise-associated cellular immune capacities,effect of Radix Astragali injection and Aerobic exercise on quantities of peripheral T lymphocyte subsets in rats was observed.Method:50 Wister Rats were randomLy divided into 5 group:quiet control group（n = 10）,once exhausting exercise group（once exhausting exercise before drawning materials）（n = 10）,Aerobic exercise and once exhausting exercise group（once exhausting exercise after aerobic exercise for four weeks）（n = 10）,Radix Astragali injection and once exhausting exercise group（once exhausting exercise after oraling Radix Astragali injection for four weeks）（n = 10）,Radix Astragali injection and Aerobic exercise and once exhausting exercise group（once exhausting exercise after oraling Radix Astragali injection and for aerobic exercise four weeks）（n = 10）.After 4 weeks test,time of exhaustive exercise were record,percentage of peripheral T Lymphocyte subsets in circulating blood were tested by FCM,and body weight were measured before and after experiment.Result:Afer exhausting exercise,percentage of CD4 + and the ratio of CD4 + to CD8 + in Lymphocyte subsets,as well as were lower（P < 0.01）,percentage of CD8 + as well as were higher than that in normal control group（P <0.01）.The change was prevented by Radix Astragali injection and aerobic exercise;the exhausting exercise time of aerobic exercise and once exhausting exercise group,astragali injection and once exhausting exercise group,Radix Astragali injection and aerobic exercise and once exhausting exercise group as well as were longer than once exhausting exercise group（P < 0.01）,and the exhausting exercise time of Radix Astragali injection and aerobic exercise and once exhausting exercise group as well as were longer than aerobic exercise and once exhausting exercise group,Astragali injection and once exhausting exercise group（P < 0.01）.Conclusion:Radix Astragali injection and aerobic exercise may improve exercise capacity and preven immunosuppression in rats after exhaustive exercise in rats,enhance effect after combinating the two means.关键词：Radix Astragali injection;exhausting exercise;aerobic exercise;CD4 +/CD8 +
- 0
- |
- 156
- |
- 10
发布时间：2024-09-23 - 摘要：目的:观察黄芪桂蛭方防治药物流产并发症的临床疗效。方法:将128例药物流产患者随机分为治疗组予黄芪桂蛭方口服10 d,对照组不予辅助治疗。结果:治疗组总有效率92.19%,对照组总有效率70.31%。结论:黄芪桂蛭方是防治药物流产并发症的有效药物。关键词：黄芪桂蛭方;药物流产并发症;临床疗效
- 0
- |
- 36
- |
- 2
发布时间：2024-09-23 - 摘要：目的:比较经尿道前列腺电切（TURP）术与等离子体双极电切（TUPKRP）术治疗良性前列腺增生症（BPH）的临床疗效。方法:TUPKRP组98例,TURP组67例,观察平均手术时间、术中出血量、术后持续膀胱冲洗时间、留置尿管时间、住院时间、手术并发症及术前术后国际前列腺症状评分（IPSS）、生活质量评分（QOL）、最大尿流率（Qmax）、剩余尿量（PVR）等指标。结果:TURP组失血量多于TUPKRP组,P<0.05。TURP组术后血钠浓度较术前明显下降,且低于PKRP组,P<0.05。2种方法的手术时间,IPSS,QOL,Qmax,PVR等相比差异无统计学意义。与TURP术比较,TUPKRP术出血更少,无经尿道电切综合征发生。结论:TUPKRP和TURP均是治疗BPH的微创方法;TUPKRP是一种安全性高、并发症少、疗效确切、更适合人体生理功能的一种手术方法,是现代腔道泌尿外科的一个进步。关键词：经尿道前列腺切除术;等离子体双极电切术;前列腺增生
- 0
- |
- 75
- |
- 15
发布时间：2024-09-23 - 摘要：Objective:To investgate the efficacy of radioactive 125I seed implant brachytherapy for salivary malignancy.Method:Twelve patients with salivary malignancy were treated by radioactive 125 I seed implanting.Eight patients’tumors were completely resected by surgery.Two patients’tumors were part resected by surgery.Two patients did not accept the surgery because they could not tolerate surgery.Result:All the patients were followed up for 2-36 months.The volume of tumors for those with radioactive therapy and without surgery was reduced obviously.Two patients’tumors were disappeared by partially surgery plus radioactive therapy.Eight patients who accepted complete operations had no local recurrence.The local control rate was 91%.Conclusion:The radioactive 125I seed implant brachytherapy for salivary malignancy is effective.But the long-term effects need further investigation.关键词：salivary malignancy;radioactive 125I seed;brachytherapy
- 0
- |
- 59
- |
- 2
发布时间：2024-09-23 - 摘要：Objective: To investgate the efficacy of radioactive 125I seed implant brachytherapy for salivary malignancy. Method: Twelve patients with salivary malignancy were treated by radioactive 125I seed implanting. Eight patients’ tumors were completely resected by surgery. Two patients’ tumors were part resected by surgery. Two patients did not accept the surgery because they could not tolerate surgery. Result: All the patients were followed up for 2- 36 months. The volume of tumors for those with radioactive therapy and without surgery was reduced obviously. Two patients’ tumors were disappeared by partially surgery plus radioactive therapy. Eight patients who accepted complete operations had no local recurrence. The local control rate was 91%. Conclusion: The radioactive 125I seed implant brachytherapy for salivary malignancy is effective. But the long-term effects need further investigation.关键词：salivary malignancy;radioactive 125I seed;brachytherapy
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:观察糖益肾方治疗早期糖尿病肾病的疗效。方法:对确诊为早期糖尿病肾病的患者,采用随机法分为治疗组和对照组,治疗组100例给予"糖益肾方",对照组60例予以胰激肽原酶片,观察2组患者临床症状、空腹血糖（FBG）、餐后2h血糖（PBG）、糖化血红蛋白（HbA1C）、尿微量白蛋白（MAlb）、尿白蛋白/尿肌酐（MAlb/Cr）、尿转铁蛋白（TRF）、血脂（TC,TG,HDL,LDL）等变化,同时检测安全性指标:血、尿常规、肝功能（ALT,AST）、肾功能（BUN,Cr）。结果:治疗组98例,在改善临床症状,HbA1C,Malb,MAlb/Cr,TRF,改善血脂代谢等方面均优于对照组（P<0.05或P<0.01）。结论:糖益肾方能降低早期糖尿病肾病患者的Malb,MAlb/Cr,TRF及HbA1C,改善脂代谢,对早期糖尿病肾病有较好疗效。关键词：糖益肾方;糖尿病肾病
- 0
- |
- 117
- |
- 2
发布时间：2024-09-23 - 摘要：Objective:To study the injection of sodium ferulate combined with Cordyceps Gushen Guiqi drink diabetic nephropathy.Method:Ninty-eight Patients were randomLy divided into three groups of diabetic nephropathy after admission all patients high-quality low-protein diabetic diet,adequate exercise,according to blood glucose levels to metformin 1.0-1.5 g.d-1 or glipizide up to 15-75 mg.d-1,hypertension Captopril 25-75 mg.d-1 divided 2-3 times orally,the control group with sodium ferulate was based on the use of sodium ferulate,14 days to a course of treatment,sharing 3 courses.the treatment group was based on the use of sodium ferulate combined injection Cordyceps Guiqi Gushen drink,14 days to a course of treatment,sharing 3 courses were evaluated.Result:Before and after treatment FBG,24 h urinary protein,blood BUN,Scr,serum Alb comparison,the differences were very significant（P《0.05 or P < 0.01）;control group FBG,24 h urinary protein before and after treatment,There was a significant difference（P《0.05 or P < < 0.01）;the indicators after treatment compared to the treatment group than the control group,the difference was significant（P《0.05） shows injection of sodium ferulate combined Cordyceps Guiqi Gushen drink treatment and delay the progression of diabetic nephropathy in synergy,efficacy significantly.关键词：diabetic nephropathy;sodium ferulate injection;Cordyceps Gushen Guiqi drink
- 0
- |
- 61
- |
- 4
发布时间：2024-09-23 - 摘要：Objective: To study the injection of sodium ferulate combined with Cordyceps Gushen Guiqi drink diabetic nephropathy. Method: Ninty- eight Patients were randomLy divided into three groups of diabetic nephropathy after admission all patients high-quality low-protein diabetic diet, adequate exercise, according to blood glucose levels to metformin 1.0- 1.5 g·d-1 or glipizide up to 15- 75 mg·d-1, hypertension Captopril 25- 75 mg·d-1 divided 2- 3 times orally, the control group with sodium ferulate was based on the use of sodium ferulate,14 days to a course of treatment, sharing 3 courses. the treatment group was based on the use of sodium ferulate combined injection Cordyceps Guiqi Gushen drink, 14 days to a course of treatment, sharing 3 courses were evaluated. Result :Before and after treatment FBG, 24 h urinary protein, blood BUN, Scr, serum Alb comparison, the differences were very significant (P0.05 or P<0.01); control group FBG, 24 h urinary protein before and after treatment, There was a significant difference (P0.05 or P<<0.01); the indicators after treatment compared to the treatment group than the control group, the difference was significant (P0.05) shows injection of sodium ferulate combined Cordyceps Guiqi Gushen drink treatment and delay the progression of diabetic nephropathy in synergy, efficacy significantly.关键词：diabetic nephropathy;sodium ferulate injection;Cordyceps Gushen Guiqi drink
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：目的:观察苁蓉益肾颗粒治疗肾虚型慢性肾小球肾炎蛋白尿的疗效。方法:口服苁蓉益肾颗粒治疗34例肾虚型慢性肾小球肾炎蛋白尿患者12周,观察治疗前后患者主要症状的变化,并测定治疗前后患者24 h尿蛋白量及肾功能。结果:与治疗前比较,苁蓉益肾颗粒能显著改善肾虚型慢性肾小球肾炎患者的主要症状（P<0.01）,能降低患者24 h尿蛋白量（P<0.05）。结论:苁蓉益肾颗粒对肾虚型慢性肾小球肾炎蛋白尿有较好的治疗作用。关键词：苁蓉益肾颗粒;蛋白尿;肾小球肾炎
- 0
- |
- 207
- |
- 21
发布时间：2024-09-23 - 摘要：Objective: To observe the the therapeutic effect on peripheral blood leukocyte reduction of AIDS by Jingyuankang Capsule. Method: 116 cases of HIV/AIDS patients were divided into two groups: 58 cases in the treatment were treated by Jingyuankang Capsule and likejun mimic tablet with HAART therapy, and the other 58 cases in the control by likejun tablet and mimic Jingyuankang Capsules with HAART. The design was a randomized double-blind research, and 6 months as a course. Peripheral blood leukocytes were monitored for these cases. Result: Jingyuankang Capsule treatment for AIDS showed good effect, whether they had taken with AZT (HAART) or not. HAART scheme showed that it could improve peripheral blood leukocytes. Jingyuankang Capsule appeared safe and effective. Through clinical investigation and follow-up the capsule showed no toxicity and adverse reaction. Conclusion: Jingyuankang Capsules can effectively treat AIDS patients with reduction in peripheral blood leukocyte.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the the therapeutic effect on peripheral blood leukocyte reduction of AIDS by Jingyuankang Capsule.Method:116 cases of HIV /AIDS patients were divided into two groups:58 cases in the treatment were treated by Jingyuankang Capsule and likejun mimic tablet with HAART therapy,and the other 58 cases in the control by likejun tablet and mimic Jingyuankang Capsules with HAART.The design was a randomized double-blind research,and 6 months as a course.Peripheral blood leukocytes were monitored for these cases.Result:Jingyuankang Capsule treatment for AIDS showed good effect,whether they had taken with AZT（HAART） or not.HAART scheme showed that it could improve peripheral blood leukocytes.Jingyuankang Capsule appeared safe and effective.Through clinical investigation and follow-up the capsule showed no toxicity and adverse reaction.Conclusion:Jingyuankang Capsules can effectively treat AIDS patients with reduction in peripheral blood leukocyte.
- 0
- |
- 222
- |
- 8
发布时间：2024-09-23 - 摘要：Objective:To observe the clinical curative effects of acupuncture combined with Chinese herbal medicine in treating chloasma.Method:One hundred and seventy-six patients with chloasma were randomly divided into two groups:90 cases in the treatment group were treated by acupuncture combined with Chinese herbal medicine,86 patients were using vitamins（E and C） as the control.The effect was observed after the three courses of treatment.Result:The total effective rate in treatment group and control group were 96.7% and 82.6% respectively.There appeared a significant difference（P < 0.05）.Conclusion:Combined treatment of acupuncture with Chinese herbal medicine for chloasma is obviously superior to vitamines.
- 0
- |
- 257
- |
- 15
发布时间：2024-09-23 - 摘要：Objective: To observe the clinical curative effects of acupuncture combined with Chinese herbal medicine in treating chloasma. Method: One hundred and seventy-six patients with chloasma were randomly divided into two groups: 90 cases in the treatment group were treated by acupuncture combined with Chinese herbal medicine, 86 patients were using vitamins ( E and C) as the control. The effect was observed after the three courses of treatment. Result: The total effective rate in treatment group and control group were 96.7% and 82.6% respectively. There appeared a significant difference (P<0.05). Conclusion: Combined treatment of acupuncture with Chinese herbal medicine for chloasma is obviously superior to vitamines.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Bufonis venenum and bufonis corium have been widely used in the Chinese Traditional Medicine for a long history. To investigate the chemical differences among the bufonis venenum, bufonis corium,and bufonis periostracum, the progress on the chemical constituents in the different medicinal parts from toads, such as venom and skin, was summarized. The chemical constituents mainly included bufadienolides, indole alkaloids, steriods, and etc. More than one hundred bufadienolides and twelve indole alkaliods were reported and 34 references were cited in the present review.关键词：Toad;chemical constituents;bufadienolides;indole alkaliods;bufogargarizanine
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Bufonis venenum and bufonis corium have been widely used in the Chinese Traditional Medicine for a long history.To investigate the chemical differences among the bufonis venenum,bufonis corium,and bufonis periostracum,the progress on the chemical constituents in the different medicinal parts from toads,such as venom and skin,was summarized.The chemical constituents mainly included bufadienolides,indole alkaloids,steriods,and etc.More than one hundred bufadienolides and twelve indole alkaliods were reported and 34 references were cited in the present review.关键词：Toad;chemical constituents;bufadienolides;indole alkaliods;bufogargarizanine
- 0
- |
- 1458
- |
- 45
发布时间：2024-09-23 - 摘要：The methods and applications of fluorescence labeling on macromolecules from traditional Chinese medicine(TCM) were reviewed in this paper. Potential application of biophotonic techniques in activity tracing of TCM was discussed. This may clarify the mechanism of macromolecule from TCM and propose new ideas for fluorescence labeling unknown multicomponent of TCM macromolecules.
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：The methods and applications of fluorescence labeling on macromolecules from traditional Chinese medicine（TCM） were reviewed in this paper.Potential application of biophotonic techniques in activity tracing of TCM was discussed.This may clarify the mechanism of macromolecule from TCM and propose new ideas for fluorescence labeling unknown multicomponent of TCM macromolecules.
- 0
- |
- 835
- |
- 15
发布时间：2024-09-23 - 摘要：Based on the literatures of Chinese journal full-text database in China national knowledge infrastructure during 1954- 2010, the annual distribution, periodicals distribution, citation frequency, subjects distribution, authors and research institutes distribution of the literatures about Dendrobium were analyzed statistically by the method of bibliometrics. The results indicate that the course of Dendrobium study in China can be divided into 5 stages—starting stage(including 2 stages), standing stage, stable stage and rapid upstroke stage. Dispersion and concentration coexisted in the journal distribution, the core journal research groups have been already formed and take the following three journals as representation—The Chinese Medicine Magazine of China, Chinese Traditional and Herbal Drugs and Shaanxi Journal of Traditional Chinese Medicine. The research subjects have a wide array of sources, including clinic, pharmacology, tissue culture, seedling propagation, component analysis and so on. The research institutes and teams have been already formed. Dendrobium officinale,Dendrobium nobile and Dendrobium huoshanense were the main objects of those studies. The study of Dendrobium has showed marked achievement, but the growing industry developed slowly and the supply and demand imbalance was outstanding. It indicates that seedling propagation, artificial planting, product development, chemistry and pharmacology study will be the hot research topics and the number of research documents about them will remain rising in the future.关键词：Dendrobium;bibliometrics;literature analysis;Dendrobium officinale;Dendrobium nobile;Dendrobium huoshanense;Dendrobium devonianum
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Based on the literatures of Chinese journal full-text database in China national knowledge infrastructure during 1954-2010,the annual distribution,periodicals distribution,citation frequency,subjects distribution,authors and research institutes distribution of the literatures about Dendrobium were analyzed statistically by the method of bibliometrics.The results indicate that the course of Dendrobium study in China can be divided into 5 stages—starting stage（including 2 stages）,standing stage,stable stage and rapid upstroke stage.Dispersion and concentration coexisted in the journal distribution,the core journal research groups have been already formed and take the following three journals as representation—The Chinese Medicine Magazine of China,Chinese Traditional and Herbal Drugs and Shaanxi Journal of Traditional Chinese Medicine.The research subjects have a wide array of sources,including clinic,pharmacology,tissue culture,seedling propagation,component analysis and so on.The research institutes and teams have been already formed.Dendrobium officinale,Dendrobium nobile and Dendrobium huoshanense were the main objects of those studies.The study of Dendrobium has showed marked achievement,but the growing industry developed slowly and the supply and demand imbalance was outstanding.It indicates that seedling propagation,artificial planting,product development,chemistry and pharmacology study will be the hot research topics and the number of research documents about them will remain rising in the future.关键词：Dendrobium;bibliometrics;literature analysis;Dendrobium officinale;Dendrobium nobile;Dendrobium huoshanense;Dendrobium devonianum
- 0
- |
- 583
- |
- 18
发布时间：2024-09-23 - 摘要：Valerenic acid is a key marker compound of valerian of Valerianacea,which also is one of the main active constituents of its central inhibiting effects.Recent studies show that valerenic acid has pharmacological effects probably through acting on GABAA receptor,5-HT receptor and NF-kappaB.In this paper the research progress of the extraction and content determination,pharmacological action,pharmacokinetics and pharmacodynamics of valerenic acid were involved,which will offer references for further study of Valerenic acid.关键词：valerian;valerenic acid;review articles
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：Valerenic acid is a key marker compound of valerian of Valerianacea,which also is one of the main active constituents of its central inhibiting effects.Recent studies show that valerenic acid has pharmacological effects probably through acting on GABAA receptor,5-HT receptor and NF-kappaB.In this paper the research progress of the extraction and content determination,pharmacological action,pharmacokinetics and pharmacodynamics of valerenic acid were involved,which will offer references for further study of Valerenic acid.关键词：valerian;valerenic acid;review articles
- 0
- |
- 272
- |
- 6
发布时间：2024-09-23 - 摘要：The concept and characteristics of DNA barcoding technology,the gene standard and identify principle, operation procedure and methods of analysis,establishing new animal traditional Chinese medicine DNA barcoding identification platform were reviewed, and the application of DNA barcoding for animal traditional Chinese medicine identification was prospected.关键词：DNA barcoding;animal traditional chinese medicine;identification
- 0
- |
- 0
- |
- 0
发布时间：2024-09-23 - 摘要：The concept and characteristics of DNA barcoding technology,the gene standard and identify principle,operation procedure and methods of analysis,establishing new animal traditional Chinese medicine DNA barcoding identification platform were reviewed,and the application of DNA barcoding for animal traditional Chinese medicine identification was prospected.关键词：DNA barcoding;animal traditional chinese medicine;identification
- 0
- |
- 918
- |
- 22
发布时间：2024-09-23 - 摘要：中医学是复杂性思维的体现,《内经》是中医基础理论的奠基者,《内经》的学术思想已经蕴含了复杂性的思考。从《内经》研究的对象,提倡的整体、恒动观念,强调多脏腑相关性等,都体现了复杂性思维的基本特征。关键词：内经;复杂性科学;理论研究
- 0
- |
- 175
- |
- 7
发布时间：2024-09-23 - 摘要：<正>慢性肾衰竭（CRF）是多种原发病或继发肾脏病变持续发展的共同后果,目前缺乏有效的治疗方法。寻找有效的治疗药物,延缓CRF发展是临床急待解决的重要课题。中药对肾脏治疗作用的研究已日益引起人们的重视,我院用黄
- 0
- |
- 102
- |
- 8
发布时间：2024-09-23 - 摘要：<正>老年人肺部感染如不及时、妥善地治疗,易引起多种并发症,甚至导致多脏器功能衰竭,预后不良。头孢类抗菌素是临床常用的治疗肺部感染的药物。笔者观察到用头孢菌素治疗老年人肺部感染,配合以有针对性的护理,疗效良好。
- 0
- |
- 30
- |
- 0
发布时间：2024-09-23 - 摘要：<正>近年来再生障碍性贫血（AA）发病呈上升趋势,由于机体抵抗力极低,容易并发多种疾病,带状疱疹就是一种比较常见的并发症。带状疱疹系由水痘-带状疱疹病毒感染所引起的一种急性皮肤病。常突然发生,沿一侧周围神经呈带状分布,成群聚集性小水疱,常伴神经痛;以皮肤红斑,灼热刺痛,簇集水疱为主要特征,我科自2005年1月至2008年12月应用西黄丸治疗AA并发带状疱疹40例取得明显疗效。
- 0
- |
- 126
- |
- 6
发布时间：2024-09-23 - 摘要：<正>血清总胆固醇（TC）水平升高与冠状动脉粥样硬化性心脏病（CHD）的发生及病变的进展密切关联。在改善冠状循环血流的同时,积极控制血清TC的升高,对CHD的治疗具有重要意义。我们在扩张冠状动脉、降低血脂、抗血小板聚集等西药治疗的基础上,加用中药治疗CHD中稳定性心绞痛合并高胆固醇血症的患者,取得了满意疗效。
- 0
- |
- 58
- |
- 8
发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术
- 0
- |
- 26
- |
- 0
发布时间：2024-09-23 - 摘要：<正>东莞广州中医药大学中医药数理工程研究院成立于2007年7月,是由东美市人民政府与广州中医药大学共同出资组建的产学研合作创新平台,座落于风光旖旎旅的松山湖畔。研究院是实行企业化管理的事业单位,全面执行项目负责制,以团队形式整合研究资源,运作灵活、高效,积聚了中医理论、中医临床、中药新产品研发、中
- 0
- |
- 42
- |
- 0
发布时间：2024-09-23
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0