最新刊期
卷 16 ， 期 15 ， 2010
- 摘要：Objective:To investigate the optimum conditions and parameters of macro reticular resin in purification of flavonoids in BYHWD.Method:Through the static adsorption and desorption experiments and the dynamic adsorption and desorption experiments,the ideal resins for BYHWD flavonoids from the D101,DA201,AB-8 and polyamide were screened.Through single-factor analysis to study the optimum conditions of resin in separating and purifying BWD flavonoids.Result:After purification by D101,BYHWD the purity of flavonoids could reach 63.1%,transfer rate was 80.2%.The static adsorption capacity of D101 macroporous resin for BYHWD flavonoids was 66.28 mg.g-1（dry resin）,the best dynamics on the sample size was 20 mL.g-1（total flavonoids concentration 3.033 mg.mL-1）,the sample flow rate was 1 BV.h-1,elution ethanol concentration was 60%,elution speed was 1.5 BV.h-1.Conclusion: D101 macroreticular resin could be better used for the separation and purification of BWD flavonoids.关键词：macroporous resin;BYHWD;flavonoids;separation;purification;D101
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发布时间：2024-09-23 - 摘要：Objective: To optimize the formula of total alkaloids of Aconitum sinomontanum Nakai sustained-release tablets and evaluate its drug release property.Method:Orthogonal design was used to obtain the best formula.The best formula was determined by dissolution of lappaconitine in total alkaloids of Aconitum sinomontanum Nakai sustained-release tablets and comprehensive scoring method.The release formula was made to investigate the release mechanism.Result:The dissolution curves in-vitro showed that the drug release could be best described by the Higuchi equation（Q = 0.349 t1/2-0.150 5,r = 0.997 2） and Peppas equation（lnQ = 0.688 9 lnt-1.595 7,r = 0.995 5）.It showed that the release mechanism were Fick diffusion and backbone corrosion.The release rate matched the requirement of quality control on sustained-release tablets in Chinese Pharmacopoeia（2005）.Conclusion:The method of content determination was simple and sustained-releasing rate was significant.The release mechanism followed the kinetic model of sustained-release tablets.It is worth doing further research for clinical use.关键词：total alkaloids of Aconitum sinomontanum Nakai;sustained-release tablets;release rate
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发布时间：2024-09-23 - 摘要：目的:建立常春油麻藤中大豆黄素的含量测定方法。方法采用正交试验对大豆黄素含量测定的提取条件进行系统研究。结果:甲醇为提取溶剂,固液比1∶15（g∶mL）,85℃回流提取3次,每次1.5 h,常春油麻藤中大豆黄素含量为0.004 47%。结论:优化确定了常春油麻藤中大豆黄素的提取方法,建立了相关的含量测定方法。关键词：常春油麻藤;大豆黄素;RP-HPLC法
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发布时间：2024-09-23 - 摘要：目的:利用响应面分析法优化脑络安胶囊研制渗漉提取工艺条件。方法:在单因素实验基础上选取实验因素与水平,选择中心组合实验设计,考察乙醇体积分数、粉碎度、浸提时间3因素对葛根素质量浓度的影响,依据design expert7.1回归分析确定各工艺条件。结果:根据实验因素的显著性和交互作用分析及最优化,得出最佳工艺条件为:乙醇体积分数为70%,粉碎度为1 030μm,浸提时间为37 h。结论:结论该优选出的提取工艺合理,可为工业生产提供依据。关键词：葛根素;渗漉;响应面分析;提取工艺
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发布时间：2024-09-23 - 摘要：Objective:Study the optimum condition of extraction of licorice flavonoids from licorice waste residue and explore the feasibility of development and utilization of licorice waste residue.Method:Organic solvent extraction,ultrasonic-assisted organic solvent extraction,basic solvents extraction and ultrasonic-assisted basic solvent extraction were used to extract licorice flavonoids from licorice waste residue.Optimum condition for extraction was selected with theorthogonal design,using the yield,content and α-glucosidase inhibitory activity of licorice flavonoids from all extracts as the evaluating criteria.Result: The optimum extracting condition with ultrasonic-assisted organic solvent extraction method was that the ethanol concentration was 65%,ratio of material to solvent was 1∶ 12,extraction time was 1.5 h,and extraction temperature was at 75 ℃.Conclusion:The development and utilization of extracting licorice flavonoids from licorice waste residue was feasible with good social and economic benefits.关键词：icorice waste residue;licorice flavonoids;ultrasonic-assisted organic solvent extraction;Orthogonal Design;α-glucosidase inhibitory activity
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发布时间：2024-09-23 - 摘要：Objective:To establish a method for determination of chlorogenic acid in Lonicera japonica leaves by HPLC,and study on extracting and purifying chlorogenic acid from Lonicera japonica leaves by different macroporous resin,so as to provide a reference for the comprehensive development and utilization of honeysuckle herbs.Method: The chlorogenic acid was determined with the detection wavelength 327 nm by HPLC.Static adsorption and dynamic adsorption were used to optimize the adsorption and elution process by macroporous resin.Result:Chlorogenic acid was linear in the range of 2.376 8-60.476 9 μg.The average recovery was 96.82%,RSD was 1.83%.At the same time,the purification effect of chlorogenic acid by HPD450 macroporous resin was better than other resins,the optimal conditions were:column diameter ratio 3 ∶ 1,liquid concentration 0.5-1.0 g·mL-1,absorption velocity 0.5 BV·h-1,resin adsorption quantity 0.3 g·g-1,elution solvent 30% ethanol,elution speed 2 BV·h-1,elution solvent volume 2 BV.Conclusion:The process of purification of chlorogenic acid from Lonicera japonica leaves by HPD450 macroporous resin was stable and could be used for industrial production.关键词：Lonicera japonica leaf;chlorogenic acid;Macroporous resin;HPLC
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发布时间：2024-09-23 - 摘要：Objective: To optimize the formulation and preparation process of Zhi-kang orally disintegrating tablets,and establish an HPLC method for determination of emodin and chrysophanol in the tablets.Method:The prescription of Zhi-kang orally disintegrating tablets were optimized by parallel design technique,with disintegrating time,appearance and taste as the assessment index.The analytical column was packed with Kromasil C18（200 mm × 4.6 mm,5 μm） and performed at 35 ℃.The mobile phase was consisted of methanol-0.2% formic acid（82∶ 18）.The flow rate was 1.0 mL.min-1.Result:The tablets prepared were fine in appearance and taste,disintegrated within 1 min.Emodin and chrysophanol were linear in the range of 0.100 5-0.201 0 μg and 0.040 2-1.608 0 μg respectively.The average recovery was 99.44%（emodin） and 98.18%（chrysophanol）.Conclusion: The disintegrating tablets were designed rationally and feasible to operate.HPLC assay was simple,accurate and specific.关键词：orally disintegrating tablets;HPLC;emodin;chrysophanol
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发布时间：2024-09-23 - 摘要：Objective: To seek the optimum alcohol extraction conditions of Weikan tablets.Method:The extraction conditions were optimized by orthogonal tests with the extract yield and the amount of the extracted hydrochloride berberine as index.Result: The optimum extraction process was as follows: soaking in alcohol for 1.5 h,extracting two times,adding alcohol for 8,6 times and extracting for 2.5,2.0 h respectively.Conclusion: The optimum extraction process was stable and practicable in manufacture.关键词：Weikan tablets;orthogonal tests;extraction process;hydrochloride berberine
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发布时间：2024-09-23 - 摘要：Objective: Flavonoids were extracted from Toona sinesis successfully.Method:Flavonoids were extract,By using the method of dissolved in alkali and separated out in acid and microwave.All the flavonoids in Toona sinesis were determined by UV-spectrophotometry with rutin as the standard and the quantities of flavonoids component were measured.And use L9（34） orthogonal test design.Result:It showed that the optimum extracting conditions were determined with 0.5%（M /V） Na2B4O7.10 H2O as extractor with the ratio of material to liquid at 1 g∶ 40 mL,and extracting for 30min /time and the separation temperature is 60 ℃.Conclusion:This study provided scientific basis for Toona sinesis,development and utilization of gardenia.关键词：Toona sinesis;Flavonoids;extraction;Determination
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发布时间：2024-09-23 - 摘要：Objective: To develop a new method for simultaneous determination of two hydrophillc components and three lipophillc components of Radix et Rhizoma Salviae Miltiorrhizae.Method:The HPLC-double wavelength detection was employed using a column of Dikma Diamonsil C18（4.6 mm × 250 mm,5 μm） with a mobile phase of acetonitrile（A）-0.05% phosphoric acid（B）.The gradient elution program was as follows:0-27 min,20%-25% A;27-55 min,25%-80% A;55-65 min,80%-80% A.The detection wavelength was set at 270 nm and 286 nm.The column temperature was at 30 ℃.Result:Rosmarinic acid was linear in the range of 1.92 × 10-2-0.96 μg;salviamolic acid B was linear in the range of 7.29 × 10-2-3.64 μg;clyptotanshinon was linear in the range of 3.05 × 10-3-0.15 μg;tanshinone was linear in the range of 1.71 × 10-3-8.55 × 10-2 μg and tanshinone ⅡA was linear in the range of 5.80 × 10-3-0.29 μg.The average recovery was 99.88%,104.75%,101.08%,101.41%,102.41% respectively.Conclusion:The present method was simple and had satisfactory efficacy;it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.关键词：Salvia miltiorrhiza Bge.;rosmarinic acid;salviamolic acid B;clyptotanshinone;tanshinone Ι;Tanshinone ⅡA;high performence liquid chromatography
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发布时间：2024-09-23 - 摘要：Objective:The contents in different samples and different parts of the beetle were determined to study the impact of processing blister beetle on the total cantharidin contents.Method:HPLC conditions were as follows:the mobile phase was methanol-water（23∶77）;the column temperature was at 35℃;the flow rate was 1.0 mL.min-1;the detection wavelength was at 230 nm.Result: The total cantharidin contents determined were: unprocessed mylabris>blister beetle>mylabris（stir-frying with rice）.The total contents of head,legs,inside and outside wings of blister beetle were 0.61%.Conclusion:For safety,the total cantharidin contents should be used to control the quality of blister beetle.关键词：blister beetle;total cantharidin;before and after processed;HPLC
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发布时间：2024-09-23 - 摘要：Objective: To analyze the components of urine and feces from Trogopterus.Method:To collect the same group Trogopterus urine and feces feed by Cacumen platycladi.Extract by N-hexane and Analysis by GC-MS.Result:The components of urine and feces Trogopterus,were very different more small molecular supersession components and Cacumen Platycladi ingredients exit in the urine.Conclusion: The traditional Chinese medicine experience of using this material is proper and scientific.关键词：Trogopterus xanthipes;mixture of urine and feces;feces;urine;GC-MS
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发布时间：2024-09-23 - 摘要：Objective: To study the chemical constituents of Flos Genkwa,providing control substance for the research of Flos Genkwa and Flos Genkwa processed with vinegar.Method: 80% ethanol extract of Flos genkwa was obtained by solvent method,chemical constituents were isolated by macroporous resin HP-20,silica gel column and Sephadex LH-20 column chromatography.Their structures were determined by physiochemical and NMR spectral data.Result: Eleven compounds were isolated from ethanol extract of Flos Genkwa,and identified as genkwanin（1）,apigenin（2）,luteolin（3）,luteolin-7-O-β-D-glucoside（4）,quercetin（5）,daphnoretin-7-O-β-D-glucoside（6）,kaempferol-3-β-D-（6-O-trans-p-coumaroyl） glucopyranoside（7）,4′,7-dimethyl-apigenin（8）,daphnoretin（9）,β-sitosterol（10）,daucosterol（11）.Conclusion:Compounds 4,5,6 were first isolated from Flos Genkwa.关键词：Flos Genkwa;chemical constituents;flavonoids
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发布时间：2024-09-23 - 摘要：Objective: Comparing the content of flavonoid in different harvesting periods and to evaluate the optimal harvest time.Method:Extraction of flavonoid with ultrasonic.The content of flavonoid was compared by UV at wavelength of 508 nm.Result:Rutin was linear in the range of 22.78-113.88 mg.L-1（r = 0.999 8） with average recovery of 98.87% and RSD of 1.03%（n = 9）.The measurement results of Poacyzuan hendersonii leaves in different harvesting time:relative low in April and May,unfaltering rose in June to September,little lower in October than in September.Conclusion:The method was simple,accurate,reproducible,it can be used to evaluate the best harvest time of P.hendersonii,and provide a scientific basis for rational development and utilization.关键词：Poacyzuan hendersonii;flavonoid;harvesting periods;UV
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发布时间：2024-09-23 - 摘要：Objective:To investigate the volatile constituents in Mayi Rhinitis Spray and provide some useful information for the quality.Method: The volatile components of Mayi Rhinitis Spray were separated by GC-MS and identified by comparing the structural information given by the MS database.Area normalization method was used to calculate the relative content of each compound of the volatile components.Result: Thirty four compounds were separated,27 compounds were identified,accounting to 95.5% of the total essential components.Conclusion: The principle constituents of the volatile components were eucalyptol（19.85%）,borneol（16.32%）,camphor（12.54%）,isoborneol（8.4%）,methyl eugenol（6.08%）,sabenene（4.19%）.关键词：Mayi Rhinitis Spray;volatile components;GC-MS
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发布时间：2024-09-23 - 摘要：Objective: To set up the method for content determination of chlorogenic acid of Maxin oral.Method: HPLC was adopted performing on a kromasil C18 column（4.6 mm × 250 mm,5 μm） with acetonitrile-0.1%（w） phosphoric acid（10∶ 90） as the mobile phase at a flow rate of 1.0 mL.min-1.The detective wave length was set at 327 nm and the column temperature was at 30℃.Result:Chlorogenic acid was linear in the range of 15.84-95.04 mg·L-1.The standard curve equation was Y = 27.606 X + 27.927,r = 0.999 5.Conclusion: The method set up was stable,feasible and accurate,and it can be used to control the quality of Maxin oral.关键词：Maxin oral;chlorogenic acid;content determination
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发布时间：2024-09-23 - 摘要：Objective: To establish a method for the quality control of the Tongsaiyinao Oral Liquid.Method: TLC method was used to identify Dilong,Chuanxiong,and Yuanhu.HPLC was adopted to determine the ferulic acid in Chuanxiong.Result: The characteristic spots of these crude drugs could be detected in TLC color spectrum.Ferulic acid was linear in the range of 15.28-152.80 mg.L-1.The average recovery was 99.38% RSD 1.11%（n = 6）.Conclusion:The method was accurate and reliable with good reproducibility,and can be used as a quality control of Tongsaiyinao Oral Liquid.关键词：HPLC;ferulic acid;Tongsaiyinao Oral Liquid
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发布时间：2024-09-23 - 摘要：Objective: To establish the method for quality control of Gualouxiebai Granules.Method: Macrostemonoside B in this medicine was determined by HPLC.Result: The regression equation of the macrostemonoside B was Y = 1.446 3X + 4.916 5,the linear range was 1.095-17.52 μg,r = 0.999 6.The average recovery was 97.85% with RSD of 1.86%.Conclusion: The method can be applied to the quality control of Gualouxiebai Granules.关键词：Gualouxiebai Granules;macrostemonoside B;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To compare the influences of four kinds of extraction methods in current standard of proprietary Chinese medicines on the determination of magnolol and honokiol content.Method: The samples were extracted by petroleum ether and methanol using ultrasonic extraction or Soxhlet extraction;determination was performed by HPLC.The column was Kromasil C18（4.6 mm × 250 mm,5 μm）,water-acetonitrile-acetic acid（60∶38∶2） was used as mobile phase;UV detection was at 294 nm.Result:The content of magnolol and honokiol with ultrasonic extraction by methanol was higher than Soxhlet extraction by methanol,and the Soxhlet extraction by methanol was higher than Soxhlet extraction by petroleum ether and ultrasonic extraction in the recovery aspects.he ratio of honokiol to magnolol in samples was 0.304-2.669.Conclusion:The ultrasonic extraction by methanol for the determination was the optimal extraction methods.The different ratio of honokiol to magnolol was due to the different ingredients from different source.关键词：Magnoliae Officinalis Cortex;magnolol;honokiol;HPLC;determination
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发布时间：2024-09-23 - 摘要：Objective:To determine the contents of active ingredients（magnolia and honokiol） in Magnolia officinalis processed by different methods and from different areas by HPLC.Method: HPLC was applied to determine the contents of magnolia and honokiol in M.officinalis processed by different methods and from different areas.The samples were performed on a Phenomsil C18（4.6 mm × 250 mm,5 μm） column with mobile phase of CH3OH-H2O（78∶22）at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 294 nm.The column temperature was 25 ℃ and the injection volumn was 10 μL.Result:There are some difference on contents of active ingredients in M.officinalis processed by different methods and from different areas.Conclusion:The method is convenient.accurate and suitale for separation of magnolia and honokiol.It can be used for determination of magnolia and honokiol in M.officinalis.关键词：Magnolia officinalis;magnolia;honokiol;HPLC;processed
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发布时间：2024-09-23 - 摘要：Objective: To establish a method for quantitative determination of mercury and arsenic in Cynomorium songaricum by using intermittent-flow-hydride generation atomic fluorescence spectrometry（HG-AFS）.Method: The method of determination was set up by atomic fluorescece spectrometry method after digesting by perchloric acid combining nitric acid.Result: Mercury was linear in the rang of 0.1-0.5 ng·mL-1,the linear function was Y = 3.54 × 102X + 9.54,r = 0.999 7,the detection limits of mercury was 0.022 ng·mL-1.Arsenic was linear in the rang of 1.0-10.0 ng.mL-1,the linear function was Y = 1.65 × 102X-3.89,r = 0.999 9,the detection limits of arsenic was 0.151 ng.mL-1.Conclusion:This method was simple and quick,the results wereaccurate and reliable for quantitative determination of mercury and arsenic in Cynomorium songaricum.关键词：hydride generation;atomic fluorescece spectrometry method;Cynomorium songaricum Rupr;mercury;arsenic;content
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发布时间：2024-09-23 - 摘要：Objective:To determine Determination ginsenoside Rg1 and notoginsenoside R1in Yirenchangtai Capsules by HPLC.Method:The separation was performed on Agilent ZE-C18 column,using methyl cyanide-water（17∶83）as mobile phase.The detection wavelength was at 203 nm.The flow rate was 1.0 mL·min-1.Result:The linear range of ginsenoside Rg1 was 0.4-10.1 μg（r=0.999 6）,The linear range of notoginsenoside R1 was 0.1-0.25 μg（r=0.999 6）.The average recovery of ginsenoside Rg1 was 99.96%,RSD was 0.25%（n=6）.The average recovery of notoginsenoside R1 was 100.03%,RSD was 0.23%（n=6）.Conclusion: The method can determine the ginsenoside Rg1and notoginsenoside R1 The method was reliable and accura.关键词：Yirenchangtai Capsules;Ginsenoside Rg1;notoginsenoside R1
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发布时间：2024-09-23 - 摘要：Objective:This investigation was performed to develop a method to prepare liquiritigenin using acid hydrolysis.Method: Preliminary test was performed to see if purified liquiritin can be converted into liquiritigenin by acid hydrolysis,then liquiritin from Glycyrrhiza uralensis was converted into liquiritigenin use the same method.TLC,UV and HPLC were used to judge feasibility of this method.Result:The conversion rate of the purified liquiritin was 81.1%,and the conversion rate of the liquiritin from G.uralensis was 63.2%.Conclusion: This method is feasible,concise and results in high extraction rate.关键词：liquiritin;acid hydrolysis;liquiritigenin
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》由国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的学术刊物,已成为"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊";"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术期刊光盘版"全文收录期刊;并被
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发布时间：2024-09-23 - 摘要：<正>《中国中药杂志》系中国科协主管,中国药学会主办,中国中医科学院中药研究所承办的综合性中药学术期刊。创刊于1955年7月,是创早最早、发行量最大的中药学术刊物。《中国中药杂志》全面反映我国中医科研最高学术水平,主要报道该领域新成果、新技术、新方法与新思路,内容包括栽培、资源与鉴定、炮制、药
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发布时间：2024-09-23 - 摘要：<正>尊敬的作者、读者:由于本刊近来投稿量不断增加,杂志影响力不断扩大,每月一刊的出版周期已无法满足广大科研工作者的投稿及发表需求。经主办单位中国中医科学院中药研究所及中国中西医结合学会中药专业委员会、主管单位国家中医药管理局及北京市新闻出版管理局的批准,本刊自
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术
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发布时间：2024-09-23 - 摘要：Objective: Comparative study on the mineral elements from wild and cultivated Sarcandra glabra.Method: Six mineral elements,K,Zn,Fe,Mg,Ca and Cu,in S.glabra were determined by microware FAAS.Result:Standard curve method was used for the determination.The experiments indicated that the content of mineral elements for human requirement（K,Ca,Fe,Mg） is rich,the content of Zn and Cu is low,for both origins of the herb.For cultivated herb,the order appears to be K>Ca>Fe>Mg>Zn>Cu;for the wild herb,the order is Ca>K>Fe>Mg>Zn>Cu.The method is highly sensitive,convenient and speedy.The recovery（n=6） is 93.8～102.5% and the RSDs（n=6） are lower than 5%.Conclusion: The methods are practicable with a good reproducibility,and the results are satisfactory.关键词：FAAS;mineral elements;Sarcandra glabra;wild;cultivated
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发布时间：2024-09-23 - 摘要：目的:建立红色金银花绿原酸、芦丁含量的测定方法,并比较红色和黄色金银花不同花期有效成分的差异。方法:对红色金银花绿原酸、芦丁含量测定方法的线性关系、检测限、定量限、精密度、稳定性、重复性、加样回收进行考察。采用HPLC法测定红色和黄色金银花中绿原酸、芦丁的含量。结果:黄色金银花绿原酸含量在花蕾期最高,而红色金银花中绿原酸含量在败花期最高。结论:红色金银花与黄色金银花在不同花期有效成分含量存在差异。关键词：金银花;绿原酸;芦丁;高效液相色谱
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of precursor feeding on cell growth and salidroside content of Rhodiola sachalinensis A.Bor.Method:Three kinds of precursor of L-phenylalanine,cinnamic acid and tyrosine were added in the initial period of cell culture and cells were harvested to measure dry weight,salidroside content,polyphenol oxidase（PPO） and phenylalanine lyase（PAL） activity in the 14 d,i e in the stability phase of the cell growth curve.Result:The best concentrations of phenylalanine,cinnamic acid and tyrosine to be added were 20,10,10 mg·L-1,while salidroside content was 2.65,3.50,3.21 mg·g-1 respectively.But high concentration of tyrosine would restrain cell growth.Conclusion:Three kinds of precursor in appropriate concentration could all promote the biosynthesis of salidroside and increase polyphenol oxidase（PPO）,phenylalanine lyase（PAL） activity,among them,cinnamic acid did work obviously.关键词：Rhodiola sachalinensis A.Bor;salidroside;precursor
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发布时间：2024-09-23 - 摘要：Objective: To study the effects of Tenebrio molitor proteins on immune function in mice.Method: Effects of T.molitor proteins on immune organs’ indexes,carbon clearance ability,delayed type hypersensitivity（DTH）,hemolysin antibody were investigated in mice.Result: T.molitor proteins could significantly increase weight of immune organs and swelling in foot sole of mice.T.molitor proteins could enhance macrophage phagocytic activity and production of hemolysin antibody.Conclusion:T.molitor proteins can enhance the immune function in mice significantly.关键词：Tenebrio molitor proteins（TS）;immune function;delayed type hypersensitivity;macrophages
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- 140
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- 9
发布时间：2024-09-23 - 摘要：Objective: The discussion straightened up the effects of the Fuzheng Huayu（FZHY） decoction on the marrow cells migrating into liver in mice fibrosis liver.Method: Based on the model of the transposition bone marrow between female and male BABL /C mice,we duplicated the CCl4 liver fibrosis mice model,and then the mice in FZHY group were administered with FZHY decoction ig for 4 weeks.Did PCR,liver function and pathology examination to view the model.,marked the Y through the FISH to observe the number of the cells from the male mice bone marrow in female mice livers,tested the chemokine SDF-1 relative expression in female mice livers by Western blot.Result: The damage of mice liver relieved obviously in FZHY decoction group compared the model group.The observation of Y chromosome dyed indicated,the number of the cells from the male mice bone marrow reduced in the FZHY decoction group compared the model group liver（P < 0.05）.The chemokine SDF-1 relative expression reduced in the FZHY decoction group compared the model group liver（P < 0.01）.Conclusion: When the liver is damaged,the FZHY decoction may be able to depress the chemokine SDF-1 expression to prevent the marrow cells to migrate into the liver,and thus in certain degree controls the marrow cell promoting the liver fibrosis.关键词：Fuzheng Huayu Decoction;bone marrow cell;cell migration;liver fibrosis
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发布时间：2024-09-23 - 摘要：Objective:To investig,ate the therapeutic effect of Anzi Mixture on concentration of positive serum anticardiolipid antibody（ACA） in cyclophosphamide-treated mice.Method:Cyclophosphamide was given to induce positive serum ACA for the model mice.Control group was given saline（0.01 mL.g-1） and distilled water（0.01 mL·g-1）;model group was received cyclophosphamide（0.01 mL·g-1）and distilled water（0.01 mL·g-1）.The treatment groups were given aspirin or high,middle,low doses of Anzi Mixture（0.01 mL·g-1） on the model of cyclophosphamide induced.All groups were administered once daily for 14 consecutive days.Eyeball blood sample was collected for all the mice in all groups 1 h after administration on the 14 th day,and then serum was separated by centrifugation,ELISA was employed to detect the serum ACA levels.Result:Compared with the model group,sig-nificant decrease in serum ACA level was observed in all groups of different dosages of Anzii Mixture（P < 0.05）.Compared to aspirin group,there was no obvious difference in serum ACA level for any group of Anzi Mixture.Con-clusion:Anzi Mixture could effectively reduce serum ACA level in cyclophosphamide-treated mice with a dose-de-pendent manner.The potency of Anzi Mixture treatment appeared to be equal to that of aspirin treatment.关键词：Anzi Mixture;immune abortion;cyclophosphamide;anticardiolipid antilody
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- 140
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发布时间：2024-09-23 - 摘要：Objective:To study,the effect of decoction of Agastache rugosa with dried tangerine peel in different proportion on gastrointestinal motility in rat model of limb ischemia-reperfusion.Method:Limb ischemiareperfusion model of gastrointestinal dysfunction was established.Decoction of Agastache rugosa with tangerine peel in different proportions was administered to observe the influence on gastrointestinal motility in ischemia-reperfusion rat and on gastrointestinal hormone abnormalities.Result:Gastrointestinal motility in the model rat was slowed down,and secretion of motilin and gastrin was decreased.Agastache rugosa and dried tangerine peel together in different proportions could improve gastrointestinal mobility in the model（P < 0.05） and increase the levels of the motilin and gastrin（P < 0.05）.Conclusion:Agastache rugosa and dried tangerine peel together in different proportions may improve gastrointestinal motility disorder in the brat model of limb ischemia-reperfusion.The mechanism may linked with improvement of gastrointestinal motility,and the increase in plasma motilin and gastrin.关键词：Decoction of Agastache rugosa with tangerine peel in different proportion;model of gastrointestinal motility disorder;gastrointestinal propulsion;motilin;gastrin
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- 300
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- 24
发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Qianliean on the treatment of benign prostatic hyperplasia and the related mechanism.Method:Rat model of benign prostatic hyperplasia was established by castration followed by injection of testosterone.The rats were randomly divided into 5 groups:the normal group,the model group,the Qianliean high-dose group,the Qianliean low-dose group,Longbishu group.The rats were treated accordingly and sacrificed after 30 d.The prostates were sampled and weighed,and the prostate index was calculated.Light microscopic and immunohistochemical specimens were prepared and investigated.Result:Prostatic weight,prostatic index and basic fibroblast growth factorlevelsin（bFGF） expression levels in three treatment groups were significantly lower than that of the model group,in particular for the Qianliean of high-dose group.Model group showed marked pathological changes in prostate tissue,Qianliean low-dose group showed alleviated pathological changes in prostate tissue compared with the model group,high-dose group and Longbishu group rats prostate tissue showed obvious improvement in pathological changes.Conclusion: Qianliean can decrease prostate wet weight in rat and alleviate pathological damage in prostate.The mechanism may be related to the inhibition of prostate tissue bFGF release.关键词：benign prostatic hyperplasia;Qianliean;basic fibroblast growth factorlevelsin
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- 142
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发布时间：2024-09-23 - 摘要：Objective:To investiga:te the mechanism of Danqi Granule for treatment of Sjogren’s syndrome（SS） in rat,and to consider expression of aquaporin-5（AQP5） as the target point.Method:Submandibular gland tissue was extracted from Wistar rat before blending and emulsification with complete Freund’s adjuvant（CFA）.After that,subcutaneous injection of DTP vaccine was conducted on the back to enhance immunity.The effects of Astragalus and Danshen on rat isolated salivary glands were investigated,the method used was Chinese herb perfusion.The expression distribution of AQP5 in submandibular salivary glands of rat with SS was shown by Western blot analysis.Result:After immunization 7 weeks of SS rats,thus pathological changes similar to the salivary gland in human SS were found.It suggested that a large number of lymphocyte cells were different invasive depth and effective immunization.Through Chinese herb perfusion on rat isolated salivary glands,it was found that Astragalus and Danshen could promote salivary gland secretion in different depth.Western blot analysis showed that there was a 42 × 103 in each groups.There was no abnormal expression of AQP5 in the control group,Compared with the control group,the expression of AQP5 in the rat model group was significant reduced（P < 0.05）;Danqi Granule of high-dose and low-dose could significantly increase the expression of AQP5（P < 0.05）.Conclusion:The results indicate Danshen can promote salivary glands slightly.Astragalus also has the similar effect.However,they promote the secretion of saliva graphics in different patterns.Danqi Granule through enhanced the release of water molecules in channel of submandibular gland,by upregulating the expression of AQP5,improving the secretive function of salivary glands and increasing saliva flow,so as to release the thirsty symptoms of SS.关键词：sjogren’s syndrome;submandibular gland;aquaporin;western blot;Danqi granule
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- 302
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Shuanggen Qingnao granules on proliferation and migration of neural stem cells（NSCs） in model of rats with vascular dementia and its treatment mechanism for vascular dementia（VD）.Method:Forty two SPF level SD rats were randomly divided into large,medium,low-dose of Shuanggen Qingnao granules groups,piracetam group,model group,sham operation group,the normal group（n = 6,each）.VD rat model was established by slowly injecting the same kinds of thrombotic emboli to the catheter of the external carotid artery.5-bromo-2-deoxyuriding（BrdU） positive cells and doublecortin（DCX） positive cells were respectively marked by immunohistochemical method and their expressions were observed by microscope.Result: Compared with model group,whether in cerebral cortex or hippocampus,BrdU positive cells of medium,low-dose of Shuanggen Qingnao granules groups and piracetam group were significantly increased（P < 0.05）;compared with piracetam group,BrdU positive cells of medium,low-dose of Shuanggen Qingnao granules groups in cerebral hippocampus were also significantly increased（P < 0.01）.DCX-positive cells in rats’SGZ district of normal group,sham operation group and medium,low-dose of Shuanggen Qingnao granules groups were significantly increased（P <0.01）;compared with the model group,DCX-positive cells of large-dose of Shuanggen Qingnao granules group and piracetam group were also significantly increased（P < 0.05）;DCX-positive cells in rats’SGZ district of medium,low-dose of Shuanggen Qingnao granules groups compared with the piracetam group were obviously increased（P < 0.01）.Conclusion:Shuanggen Qingnao granules can promote the proliferation and migration of NSCs in inner brain of VD rats.关键词：Shuanggen Qingnao Granules;vascular dementia（VD）;neural stem cell（NSC）;proliferation;migration
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- 192
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of the extract from Cestrum cocturnum on the immune function for the tumor-bearing mouse.Method:The routine methods were used to observed the carbon partical clearance,seurm hemolysin level,the contents of proteinic factor IL-2 and TNF-α of the H22 tumor-bearing mouse.Result:The high and middle dose of the extract from C.cocturnum could promote the carbon partical clearance speed and the content of the IL-2 and TNF-α.But the C.cocturnum had no obvious influence on the serum hemolysin level in the tumorbearing mouse.Conclusion:The extract from C.cocturnum could promote the cellular immune function of tumorbearing mouse.关键词：extract from Cestrum cocturnum Linn.;tumor-bearing mouse;immune function
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- 128
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发布时间：2024-09-23 - 摘要：Objective: To observe the protective effect of terpenoids from Liuyueqing（TLYQ） on immunological hepatic injury in mice.Method:Seventy-two mice were randomly divided into normal group,model group,Zadaxi positive control（4.2 × 10-3 g.kg-1·d-1） group and low,medium,high-dose TLYQ-treated group（0.5,1,2 g·kg-1.d-1）.On the first day,except the blank group,other groups were injected BCG from tail vein.The next day the mice in normal and model groups were administered with nothing,while those mice in other groups were respectively administered with different medicine.By the end of the 10 th day,model,Zadaxi and three TLYQ-treated groups were injected LPS from tail vein.10 h later the mice were weighed,blood was collected from eyeball,then all of groups were sacrificed,livers were taken out.ALT and AST in serum,percentage of CD3 +,CD4 +,CD8 + and CD4 + /CD8 + of every group were determined.Liver tissue damage with HE staining was observed by light microscope.Result:Compared with model group,high,medium-dose TLYQ could significantly increase percentage of CD3 +,CD4 + cell and CD4 + /CD8 +,decrease the ratio of CD8 + and the activity of AST,ALT.And TLYQ also could reduce the balloon degeneration,punctate,patchy necrosis and inflammatory cell infiltration.Conclusion:TLYQ has a protective effect on immunological liver injury in mice.关键词：terpenoids of Liuyueqing（TLYQ）;hepatic injury;T lymphocyte subpopulation
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- 174
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发布时间：2024-09-23 - 摘要：Objective:To investigate the analgesic mechanism of cinobufagin injection based on opioid receptor.Method: Hind paw withdrawal latency of female Kunming mice was induced by radiant heat and mechanical stimuli.Moreover,the paw withdrawal latency（PWL） was measured to serve as indicator of pain threshold.Then,influence of naloxone methiodide,the peripheral opioid receptor antagonist,on PWL increase induced by cinobufagin injection in mice was investigated.Result:The analgesic effect and PWL increase induced by of cinobufagin injection could be completely blocked by naloxone methiodide.Conclusion: The peripheral opioid receptor may play a main role in the analgesic effect of cinobufagin injection.关键词：cinobufagin;peripheral opioid receptor;analgesic mechanism
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- 250
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- 23
发布时间：2024-09-23 - 摘要：Objective: To explore the effect of alcohol extraction of eupatorium（AEOE） on ulcer index（UI）,malondialdehyde（MDA）,superoxide dismutase（SOD）,Nitric Oxide（NO）,Epidernal Growth Factor（EGF） in rats with experimental gastric ulcer.Method: The rat model of experimental gastric ulcer was established by giving glacial acetic acid.The levels of MDA,SOD,NO and EGF in blood serum were measured.The level of NO in stomach homogenate and UI were measured and calculated.At last,the effect of AEOE for the rats of experimental gastric ulcer was studied.Result: Compared with control group,AEOE groups and cimetidine tablet group could significantly decrease UI and the level of MDA（P < 0.01）,and significantly rise the level of SOD and NO in blood serum（P < 0.01）.AEOE groups could significantly rise the level of NO in stomach homogenate（P < 0.01）.Conclusion: AEOE has the function of protecting gastric mucous from ulcerous injury.关键词：eupatorium;gastric ulcer;anti-oxidative stress;rat
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- 202
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发布时间：2024-09-23 - 摘要：Objective: To explore the mechanism of reducing urine by effect of Suoquan Wan（SQW） on the mRNA and protein expressions of collage Ⅰ/Ⅲ.Method: Aging SD rat model was established by subcutaneous injection of D-galaclose for 8 weeks.Treatment of SQW was carried out from the 3rd week of model establishing and lasted for 8 weeks.The mRNA and protein expressions of collage Ⅰ/Ⅲ in detrusor were investigated with RT-PCR and IHC technologies for all the groups.Result: Compared with control group,SQW could remarkably cndecrease the expressions of mRNA and protein of model rats collage Ⅰ/Ⅲ（P<0.01）.Conclusion: SQW may reduce urine by increasing the expressions of mRNA and protein of collage Ⅰ/Ⅲ,so adjust water metabolism in the body.关键词：Suoquan Wan;sub-acute aging rat;detrusor;collage Ⅰ/Ⅲ;mRNA and protein expressions
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- 169
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发布时间：2024-09-23 - 摘要：目的:研究热痛方预处理对大鼠急性肺损伤的保护作用及其机制。方法:40只健康成年SD雄性大鼠,随机分为4组:生理盐水组（NS组）、模型组（ALI组）、热痛方组（RT组）和地塞米松组（DM组）。除NS组外,每组大鼠尾iv脂多糖（LPS5 mg.kg-1）复制急性肺损伤模型,并于注射LPS 2 h后行腹腔麻醉,腹主动脉采血处死。测肺系数、外周血白细胞与血小板数,行肺泡灌洗后测灌洗液中的白细胞数,ELISA法检测灌洗液中肿瘤坏死因子α（TNF-α）的含量,并在光镜下观察肺组织形态学变化。结果:应用热痛方预先给药可明显减轻内毒素引起的肺组织形态学改变,与ALI组相比,RT组肺系数、肺泡灌洗液中白细胞数及TNF-α含量均显著降低（P<0.01或P<0.05）、外周血白细胞数升高（P<0.05）;与DM组相比,肺系数、外周血小板计数、肺泡灌洗液中白细胞数也存在差异。结论:热痛方预先给药可能通过减轻肺部的炎性渗出、改善肺部的局部微循环、抑制炎症因子TNF-α的产生等途径减轻内毒素引起的急性肺损伤。关键词：急性肺损伤;热痛方;预处理;地塞米松
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发布时间：2024-09-23 - 摘要：Objective:To explore the mechanism of Bushen Wenyang Huayu（BSWYHY） on endometriosis（EMs）.Method: The peritoneal type endometriosis in SPF-grade female rats was established.They were then divided into 4 groups: BSWY high dose group（16）,BSWYHY low dose group（16）,danazol group（16）,model group（17）,and control group（12）.Immunohistochemistry was performed to examine the expression of HIF-1α and GLUT-1 in the ectopic endometrial tissues of the EMs model rats before and after treatment,and the volume change in endometriotic lesions before and after treatment was compared.Result:After treatment with high dose and low dose of BSWY and danazol,volumes of ectopic lesions were reduced obviously（P < 0.05）,and the volume of the high dose group demonstrated the most notable reduction（P < 0.01）.The expression of HIF-1α and GLUT-1 in ectopic EMs of the model group is significantly higher than that in eutopic endometrium in the control group（P < 0.01）.The BSWYHY high and low dose group and the danazol group were able to inhibit the high expression of HIF-1α and GLUT-1 substantially（P < 0.01）,with the high dose group as the best,followed by the low dose anddanazol group（P < 0.05）.Conclusion: BSWY can be an effective measure for reducing the high expression of HIF-1α and GLUT-1 for the treatment of EMs.
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of active fraction of Bushen Xingnao Decoction（BSXND） on cerebral IL-1β and ICAM-1 expression in middle cerebral artery occlusion followed reperfusion in rats.Method: The reversible middle cerebral artery occlusion（MCAO） model was established in rats to investigate the role of BSXND in ischemia injury.The scores of neurological deficits were determined by Lorma EZ method in MCAO rats.IL-1β and ICAM-1 expression in cerebral tissue were determined by immunohisto-chemistry.Result: After cerebral ischemia for 2 h followed reperfusion for 46 h,IL-1β positive cells in cerebral hippocampus and medulla increased markedly（P < 0.05,P < 0.01）,ICAM-1 positive cells in hippocampus and cortex increased remarkably（P < 0.01,P < 0.05）,alkaloid extracted from BSXND could reduce IL-1β positive cells（P < 0.01）,glycoside extracted from BSXND could decrease ICAM-1 positive cells（P < 0.01）.Conclusion: After cerebral ischemia reperfusion,both IL-1β and ICAM-1 expression are increased,alkaloid from BSXND could inhibit IL-1β protein expression in cerebral medulla,glycoside from BSXND could inhibit ICAM-1 protein expression in cerebral cortex.Alkaloid and glycosidemay be the partial substance basis of BSXND for antagonizing inflammatory reaction after cerebral ischemia reperfusion.关键词：Bushen Xingnao Decoction（BSXND）;Active fraction;ischemia-reperfusion;neurological deficit;interleukin-1β;intercellular adhesion molecule-1
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- 109
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Gengnianchun formula（GNC） on BDNF in hippocampus of ovariectomized SD rat.Method: Ten to twelve months old SD rats were randomized into mimic sham operation group,ovariectomized（OVX） group + NS,OVX + high-dose GNC group,OVX + low-dose GNC group and OVX + Nilestriol group.A little fat around ovary was cut in mimic sham operation group rats.Ovariectomy was operated in four other groups.These rats were fed with GNC,nilestriol or NS for 12 weeks respectively.The changes learning and memory were tested by Morris water maze（MWM）.The real time PCR was performed to determine the mRNA level of BDNF in right hippocampuses.And the immunohistochemistry was performed to detect the distribution of protein of BDNF in paraffin section of left hippocampus CA3.Result:Twelve weeks after ovariectomy,the scale of BDNF mRNA and protein decreased in ovariectomized rat,but rats fed with GNC or Nilestriol had higher scale of BDNF mRNA and protein.Conclusion:GNC may increase the BDNF mRNA and protein in hippocampus,and to improve the learning and memory ability for the ovx rats.关键词：Gengnianchun formula;ovariectomy;hippocampus;BDNF
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- 112
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发布时间：2024-09-23 - 摘要：Objective: To study effect of low molecular dextran on the pharmacokinetics of Xiangdan Injection in rats.Method:The rats were divided into two groups: one was administrated with Xiangdan Injection individually（group XD） and the other was administrated with the combination of Xiangdan Injection and low molecular dextran（group XD + DY）.Plasma concentrations of danshensu of two groups were detected by HPLC.The pharmacokinetic parameters were calculated by software and statistical analysis.Result: The main pharmacokinetics parameters（α,t1/2α,CL,AUC（0-t） and K10） of danshensu had statistical differences between group XD and group XD + DY（P < 0.05）.There were no significant differences in β,t1/2β,Vc,K12 and K21 between the two groups.Conclusion: There was obvious interaction of pharmacokinetics when the two drugs were used together.It is concluded that low molecular dextran inhibits the normal metabolism of danshensu of Xiangdan Iinjection.It is necessary to change the therapeutic dose of danshensu when the drug is coinjected with low molecular dextran.关键词：Xiangdan injection;low molecular dextran;danshensu;pharmacokinetics
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- 130
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发布时间：2024-09-23 - 摘要：Objective: To compare the pharmacological effects of Peucedanum praeruptorum before and after honey roasted pieces.Method: The pharmacological experiments for expectorant,antitussive and relieving asthma effects were tested by means of mouse tracheas excretion contents of phenol red,cough induced by ammonia water and the guinea pig model induced by histamine phosphate accordingly.Result: The middle and large-dosage of the honey roasted products showed the strong expectorant and antitussive effects while the small-dose of honeyed pieces and the raw pieces exhibited the better relieving action for asthma.Conclusion: The honey roasted pieces have slightly better efficiency than the raw pieces.It primarily elucidates the processing principle of the stir-baking with honey for P.praeruptorum.关键词：Peucedanum praeruptorum;stir-baking with honey;pharmacological effect
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- 323
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发布时间：2024-09-23 - 摘要：目的:研究软肝散结胶囊对大鼠肝纤维化的防治作用。方法:选用雄性大鼠,随机分为正常对照、模型组、给药30 g·kg-1组和给药15 g·kg-1组。除正常对照组外,肝纤维化模型用4O%四氯化碳油液首次5 mL·kg-1以后每次3mL·kg-1sc,每周2次,连续8周。造模首日给药组开始给药,连续8周后麻醉大鼠,腹腔主动脉取血,测定血清AST,ALT,总蛋白（Tb）,白蛋白（ALb）含量;取肝制备10%肝匀浆,测定其丙二醛（MDA）、超氧化物歧化酶（SOD）和羟脯氨酸（HYP）水平。结果:大鼠肝纤维化损伤时,血清ALT,AST活性升高,肝匀浆MDA和肝组织HYP明显增加.Tb,ALb,肝组织SOD活性降低。软肝散结胶囊给药组与模型组比较可降低ALT,AST活性及肝匀浆HYP水平,而提高血清ALB,TB及肝组织SOD活性。结论:软肝散结胶囊对四氯化碳实验性大鼠肝纤维化具有防治作用。关键词：肝纤维化;软肝散结胶囊;大鼠
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- 166
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发布时间：2024-09-23 - 摘要：目的:研究复方红景天抗缺氧的作用。方法:小鼠以复方红景天0.75,1.5,3.0 g.kg-1 3个剂量组ig给药,阳性对照组予复方党参片,对照组予等体积蒸馏水,15 d后进行各项试验和指标检测。结果:在各种缺氧模型（常压、减压、亚硝酸钠中毒、急性脑缺血）,复方红景天可不同程度地延长小鼠存活的时间,与对照组相比有显著差异。并能减少低压缺氧小鼠的死亡数量,提高小鼠的存活率,与对照组比较有显著差异。但对大鼠减压缺氧后的血糖及血乳酸值没有明显的影响。结论:复方红景天具有明显的抗缺氧作用。关键词：复方红景天制剂;缺氧;小鼠
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发布时间：2024-09-23 - 摘要：Objective: To study the influence of the Tiaozhong Granule on the vasoactive intestinal peptide（VIP）,substance P（SP）and nitricoxide synthase（NOS） of rats with mixed reflux esophagitis.Method: The rats were randomly divided into five groups:the model group,the normal group,the Tiaozhong granule Group,the Banxia Xiexin Tang group and cisapride group.Then anastomose esophagoduodenstom was used to make the rat model of mixed reflux esophagitis.The pathohistologic examination was used to observe the changes in esophagus mucous membrane.The content of SP,NOS and VIP in the esophagus tissue was detected after 12 days by immunohistochemical technique.Result: Compared with the model group,the esophagus mucous membrane of the Tiaozhong Granule group,the Banxia Xiexin Tang group and cisapride group were renovated;the content of NOS and VIP in the rats of the Tiaozhong granule group,the Banxia Xiexin Tang group and cisapride group were increased significantly;and the content of SP decreased after treatment.The differences between the treatment groups were not significant.Conclusion: Tiaozhong granule can be applied for mixed reflux esophagitis through decreasing the content of NOS,VIP and increasing SP in the esophagus tissue.关键词：reflux esophagitis;Tiaozhong Granule;vasoactive intestine peptide;substance P;nitricoxide synthase
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发布时间：2024-09-23 - 摘要：目的:测定川芎和黄芪合用后川芎主要有效成分藁本内酯在心肌缺血大鼠体内的血药浓度及药代动力学参数。方法:舌下静脉给予3.5 mg.kg-1盐酸异丙肾上腺素诱发心肌缺血,于给药后不同时间点眼眶（眼球后静脉丛）采血1 mL,HPLC/MS法测定血浆藁本内酯含量,Win Nonlin计算药代动力学参数。结果:川芎-黄芪合用川芎的主要有效成分藁本内酯的药动学参数为:Cmax（0.737±0.181）mg·L-1;AUC（0-t）（75.20±10.35）mg·L-1·min-1;AUC（0-∞）（81.66±21.01）mg·L-1·min-1;MRT（0-t）（85.87±5.03）min;CL（0.0512±0.0118）L·min-1;t1/2（77.97±5.75）min;Vz（5.808±1.641）L·kg-1。结论:与川芎单用相比,黄芪-川芎合用后藁本内酯的AUC（0-t）显著增加,MRT（0-t）延长,说明两药配伍有利于提高藁本内酯的血药浓度及体内滞留,进而发挥其药理作用。关键词：川芎-黄芪药对;藁本内酯;药代动力学
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- 733
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- 22
发布时间：2024-09-23 - 摘要：目的:研究丹芪益肝颗粒对异烟肼（INH）和利福平（RFP）合用致肝损伤的保护作用。方法:异烟肼和利福平造成小鼠肝损伤模型,测定肝脏指数（LI）、血清谷丙转氨酶（SALT）、肝组织谷丙转氨酶（LALT）、甘油三酯（TG）、总胆固醇（TC）、丙二醛（MDA）、超氧化物歧化酶（SOD）以及谷胱甘肽（GSH）等指标,光镜观察肝脏组织学变化。结果:与模型组比较,经丹芪益肝颗粒预防性治疗后,SALT,LALT,TG,MDA含量均明显降低（P<0.05,P<0.01）,SOD活力明显增强（P<0.05）,GSH含量有所升高。病理切片观察,丹芪益肝颗粒能明显改善肝组织的病理变化。结论:丹芪益肝颗粒对异烟肼和利福平造成的小鼠肝损伤具有保护作用。关键词：丹芪益肝颗粒;肝损伤;异烟肼;利福平
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- 109
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- 2
发布时间：2024-09-23 - 摘要：目的:健脾清化方抗刀豆蛋白A（ConA）诱导的肝损伤免疫学机制探讨。方法:雄性Balb/c小鼠随机分为3组:正常组、模型组、干预组,除正常组外,按ConA 5 mg.kg-1尾iv每周3次,共2周造模。正常组和模型组给予生理盐水ig,干预组给予健脾清化方25 g·kg-1 ig,治疗4周。检测血清ALT,AST含量,PE和FITC-单克隆抗体双染色法流式细胞仪检测外周血及肝内淋巴细胞亚群比例及活化状态。结果:健脾清化方干预后降低血清ALT,AST（P<0.05）,肝内CD4+T淋巴细胞活化受到明显抑制,CD8+T淋巴细胞活性增强;结论:健脾清化方通过促使细胞因子网络紊乱状态的复常,抑制CD4+T细胞的含量及其活性,活化CD8+T细胞等作用达到抗肝损伤的目的。关键词：健脾清化方;刀豆蛋白A;肝损伤;免疫学
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- 176
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- 8
发布时间：2024-09-23 - 摘要：目的:研究清热解毒剂的解热作用。方法:采用内毒素致家兔发热,观察清热解毒剂的解热作用。结果清热解毒剂高剂量组在药后120 min体温开始降低,药后360 min时温度基本降至基础体温后维持不变。中剂量组在药后120 min体温开始下降,低剂量组在药后300 min体温开始下降,体温反应曲线呈负向弧线,解热作用与剂量成正相关。结论:清热解毒剂有明显的解热作用。关键词：清热解毒剂;内毒素;解热作用
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- 140
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- 7
发布时间：2024-09-23 - 摘要：Objective: To investigate the mechanism of Jiawei Zhenwu Tang on renal interstitial fibrosis in model of SD rats.Method: Rat renal interstitial fibrosis model was produced by unilateral ureteral obstruction（UUO）,60 rats were randomly divided into three groups: sham group,UUO group,treaement group.Some rats of each group were killed respectively at 7,14,21 days after operation.Renal index,Cr and BUN were measured.The level of renal damage by HE and Masson staining was carried out.The expression of TGF-β and FN was analyzed by immunohistochemical method.Result: The level of renal index,Cr,BUN and renal damage in UUO group were highter than those in treament group.The expression of TGF-β was significantly higter than treatment group,and FN increased also.Conclusion: Jiawei Zhenwu Tang can downregulate the level of renal index,Cr,BUN in UUO group,inhibit the expression of TGF-β and accumulation of FN.Jiawei Zhenwu Tang has active effects in anti-fibrosis.关键词：renal interstitial fibrosis;Jiawei Zhenwu Tang;TGF-β;FN
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- 237
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- 15
发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of traditional Chinese medicine aconite roots on the contents of adenosine triphosphate（ATP）,adenosine diphosphate（ADP） and adenosine monophosphate（AMP） in normal rats liver tissue and its impact on the energy charge by RP-HPLC.Method: 20 Wister rats were divided into two groups randomLy and were administered with decoction of aconite roots or saline respectively for four weeks.The contents of adenine nucleotides in rat liver tissues were analysed by RP-HPLC after four weeks.Result: The hepatic ATP in aconite roots treated group increased significantly by 70.3%（P < 0.01）,with high statistical significancy compared with that in control group,whereas the content of ADP and AMP showed no obvious differences between these two groups.The total adenylate library and energy charge（EC） levels in aconite roots treated group also increased significantly（P < 0.05） compared with that in the control group.Conclusion: Aconite roots,the hot natured Chinese herbal medicine,can boost the body energy reserves,increase the ATP content and EC level in liver tissue,and thus influence the hepatic energy metabolism.关键词：HPLC;aconite roots;ATP;ADP;AMP;EC
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- 554
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- 35
发布时间：2024-09-23 - 摘要：Objective: To establish a rapid screening and evaluating method for antibacterial active substance,and apply it to the screen of antibacterial marine active substance.Method: accelerated solvent extraction was applied during the active substance extraction procedure of biological samples,and then resazurin was used as a indicator to determine the antibacterial ability.Result: A rapid screening method for antibacterial medicine was worked out.What’s more,we found that the ethyl acetate extract of Tripnetistes gratilla,Echinometra mathaei,Culcita novaeguineae and Ophiarthrum elegans have relatively strong inhibition to the experimental bacteria.Conclusion: It is an applicable method to realize high-throughput screen and evaluation of antibacterial biological-active substance,and overcome the shortage of conventional methods such as conventional methods.关键词：resazurin;antibacterial;marine drug;rapid screening;accelerated solvent extraction
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- 339
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- 8
发布时间：2024-09-23 - 摘要：Objective: To study the mechanism of acupoint external application with "treating winter disease in summer"prescription on bronchial asthma in cavia cobaya.Method: The metabolite profiles of the urine samples of the blank control group,the asthma-model group and the acupoint administration group were detected by ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry（UPLC-Q-TOF-MS）.The data was analyzed by partial least square discriminatory analysis（PLS-DA） in SIMCA-P +.Result: The result showed that metabolite profile of the urine sample was separated obviously and had no any overlapping between the asthma-model group and the blank control group,the acupoint administration group and the asthma-model group.N4-acetylcytidine,6-methyladinosinek and an undetermined compound were confirmed initially as the marker of asthma,while methylglutarate and methyl inosine,the markers of the ‘treating winter disease in summer’prescription.Conclusion: The topic establishes a method of metabonomic analysis for urine from cavia cobaya using UPLC-Q-TOF-MS.The study shows the mechanism of metabolism for the therapy of asthma with‘treating winter disease in summe’prescription.The results will be of great helpful to explain the theory of Chinese drugs pharmaceutics using biological science of modern time.关键词：treating winter disease in summer;asthma;metabonomic;ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry;partial least square discriminatory analysis
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- 740
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- 29
发布时间：2024-09-23 - 摘要：Objective: to study the anti-lipid peroxidative effect of Boschniakia rossica extract（BRE）.Method:The total antioxidant capacity,the reactive oxygen species scavenging activity,and the malonyldialdehide（MDA） content in H2O2-or Fe2 + + H2O2-induced liver homogenate and liver mitochondria were detected by spectro-photometric method.Result: BRE elevated the total antioxidant capacity and the reactive oxygen species scavenging activity in liver homogenate and liver mitochondria,and inhibited the lipid peroxidation in liver homogenate and liver mitochondria.Conclusion: BRE has the anti-lipid peroxidative effect.关键词：Boschniakia rossica;antioxidative;lipid peroxidative
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- 177
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- 19
发布时间：2024-09-23 - 摘要：Objective: To study the intestine absorption of salvianolic acid B.Method: The isolated everted intestine model was used to study the absorption in different regions of rat intestine（duodenum,jejunum,ileum and colon）.The sample was determined with HPLC in combination with ultraviolet detector.Result: The intestine absorption rate constant increased along with the increase in concentration of salvianolic acid B.The absorption rate had no significant difference between different regions of the intestine.Conclusion: The absorption of salvianolic acid B by intestine appears to be a passive diffusion process and it does not contain a special absorption window..关键词：salvianolic acid B;intestine absorption;isolated everted gut model
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- 563
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- 21
发布时间：2024-09-23 - 摘要：Objective:To explore antipyretic and anti-inflammatory effects of QingLi Tonglin decoction.Method: The antipyretic effects of Qingli Tonglin decoction were observed by dry yeast induced fever in rats;the anti-inflammatory effects of xylene induced ear edema were observed by mouse ear swelling.Result: Qingli Tonglin decoction had good antipyretic and anti-inflammatory effects for fever induced dry yeast in rats,and could significantly inhibite ear edema induced xylene.Conclusion: Qingli Tonglin decoction had significant antipyretic and anti-inflammatory effects,espicially in the high-dose group.关键词：Qingli Tonglin decoction;antipyretic effect;anti-inflammatory effect;animal experiments
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- 124
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- 12
发布时间：2024-09-23 - 摘要：Objective: To explore the effects of water extracts from Cassia obtusifolia on blood pressure,blood lipids and kidney structure in cold-induced hypertensive mice.Method: Water extracts from Cassia obtusifolia was prepared.Mice were divided into normal control group,cold-stress control group,and cold-stress feeding groups（water extracts 5,10,20 mg·kg-1）.Control groups were given physiological saline as the control.After 18 days,blood pressure was measured by carotid intubation and the contents of serum trireactive glyceride（TG）,total cholesterol（CHO）,low density lipoprotein（LDL）,high density lipoprotein（HDL） were also detected.Kidney was observed by routine histology inspection.Result: The blood pressure in cold-stress control group increased significantly compared with normal control group（P < 0.01）.Cold-stress feeding groups’systolic pressure,diastolic pressure and mean arterial pressure were not evident compared with normal control group.The content of TG in cold-stress control group was increased compared with that in normal control group（P < 0.01）,and the content of TG,TC in cold-stress feeding groups decreased compared with that in cold-stress control group（P < 0.05）.The content of LDL and HDL in cold-stress feeding group（10 mg.kg-1） decreased compared with that in cold-stress control group（P < 0.05）,and the others had no statistical significance compared with that in cold-stress control group.Renal histologic changes caused by cold-stress showed severely enlarged glomeruli,narrowed Bowman’s spaces,and renal tubule edema.Nephrons of the cold-stress feeding groups were similar to that of the control group.Conclusion: The water extracts from C.obtusifolia possesses the effects of decreasing blood pressure and modulating blood lipidcontents and improving pathological changes of renal structure.关键词：Cassia obtusifolia;hypertensive mice;blood pressure;blood lipids;kidney
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- 788
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发布时间：2024-09-23 - 摘要：目的:通过对24例注射清开灵注射液引起的不良反应进行分析,探讨其在临床中的合理应用。结果:在24例患者中,有9例患者在给药后20 min内即出现不良反应,占37.5%,有7例在给药后的1 h内出现不良反应,占29.17%,有5例在给药1 h后出现不良反应,占20.83%,另外3例在给药3 d后出现不良反应。在24例患者中有11例患者有药物热反应,有10例患者出现皮肤反应,有3例出现过敏性休克。结论:医护人员应严格掌握清开灵注射液适应证、禁忌证、配伍禁忌及使用方法,加强用药监护,力求合理、规范、安全用药,减少不良反应的发生率。关键词：清开灵注射液;不良反应;临床研究
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- 161
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- 11
发布时间：2024-09-23 - 摘要：To explore the effect of modified Shengyu decoction on spinal cord function of cervical spondylotic myelopathy after operation.Method: A total of 68 cases diagnosed definitely and operated,were randomly divided into rehabilitation exercise treatment combined with modified Shengyu decoction group（group A）,and rehabilitation exercise treatment（group B）.Group A included 32 cases,and 36 cases were in group B.After operation,the cases were treated by anti-inflammatory,rehabilitation treatment,traditional Chinese medicine,etc.Spinal cord function of cervical spondylotic myelopathy was evaluated by JOA score at preoperative and 1,2,4,6 weeks and 3 months after operation.Result: The JOA scores for patients in both groups were significantly improved compared with that of pre-operation（P < 0.05）,but there was no significant difference between the two groups after one week.And JOA in group A were significantly better than that in group B after 2,4,6 weeks and 3months,and the difference was significant（P < 0.05）.Conclusion: Rehabilitation exercise treatment and rehabilitation exercise treatment combined with modified Shengyu decoction can improve the spinal cord function for cervical spondylotic myelopathy after operation.But the effect of combined with modified Shengyu decoction is more significant.关键词：modified Shengyu decoction;cervical spondylotic myelopathy;cervical vertebrae operation;spinal cord function;clinical research
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- 170
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- 22
发布时间：2024-09-23 - 摘要：Objective:Compared with the western treatment,the plasma brain natriuretic peptide（BNP） is used to assess the efficacy of traditional Chinese and western medicine integrated treatment to congestive heart failure（CHF）.Method: 164 patients with CHF were randomly divided into western treatment group alone（control group） and combination group（treatment group）,The level of plasma BNP were measured.Result: The level of BNP decreased in both group while it decreased more significantly in treatment group compared with control group.Treatment group in improving cardiac function is better than control group（P < 0.05）.Conclusion: Integration of Chinese and western medicine is benefit to the patients of CHF and has better clinical efficacy than purely western medicine method.It may be come true through the deceasing of the level of BNP.关键词：congestive heart failure;brain natriuretic peptide;traditional Chinese and western medicine integrated treatment
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- 147
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- 11
发布时间：2024-09-23 - 摘要：Estrogen receptor（ER） is a ligand-activated nuclear transcription factor to estradiol,and mediates most of the estrogen responses.As the estrogen widely used in clinic in recent years,there are many advances in ER research,such as the organization localization,expression,function and related diseases.In addition,researches also suggest that some mechanisms responsible for traditional Chinese medicine are closely related with ER.关键词：estrogen receptor α;estrogen receptor β;traditional Chinese medicine
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- 414
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- 20
发布时间：2024-09-23 - 摘要：The Database of adverse reaction were set up according to the data from "The Traditional Chinese Medicine Database System（1949-2007）"and "The Chinese Biomedical Database System（2008）".The key words were concluding traditional Chinese medicine（TCM） and toxicity or side effect or adverse reaction.Studies showed there were over 90% of adverse reaction owing to overdose,improper or without processing,incorrect usage of toxic drug,the confused resource or misuse of medicinal plants.This result was a base for the research of internationalization and proper usage of TCM.
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- 1372
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- 63
发布时间：2024-09-23 - 摘要：The hemostyptics with complicated mechanism are commonly used in clinic,and the study of hemostyptics is the attention-attractive issue.Owing to few side effects,and abundant resources,natural compounds from medicinal plants and traditional Chinese medicines possess unique advantages and good potential in prevention and treatment of hemorrhage.Researchers have made great efforts in the field of botanical hemostyptics,and have found some medicinal plants,natural compounds and compound preparations,such as flavonoids,alkaloids and quinones.In this article,recent advances in the research of the botanical,traditional Chinese drugs,active constituents,compound preparations and the action mechanisms of these agents were reviewed.关键词：hemostyptic drug;hemostasis mechanism;active constituent;compound preparation
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- 1798
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- 51
发布时间：2024-09-23 - 摘要：Objective:The scientific significance of the compatibility theory of traditional Chinese medicine is discussed.From the compatibility point of view,there being three levels of development of Chinese medicine in the process of traditional Chinese medicine modernization: traditional Chinese medicine by prepared pieces compatibility →chinese medicine by drug combination compatibility→Chinese medicine by molecular compatibility.A new model of molecular compatibility is proposed,and the initial approach and the theoretical framework in the research of modern Chinese medicine by molecular compatibility are discussed in the paper.关键词：molecular compatibility;modern Chinese medicine
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- 422
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- 6
发布时间：2024-09-23 - 摘要：Hemorheological parameters are closely linked with cardiovascular disease.Abnormalities of the parameters in cardiovascular diseases is one of the important pathological changes.This paper gives an overview of the relationship between hemorheological parameters and cardiovascular diseases,and the influences of some traditional Chinese single herbs and compound prescriptions on hemorheological parameters.This article may provide valuable references for future researches and development of traditional Chinese medicine treatment for cardiovascular diseases.关键词：hemorheology;traditional Chinese medicine;cardiovascular diseases
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- 413
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- 15
发布时间：2024-09-23 - 摘要：The research information on volatileoils,pterosins,aromatic compounds,water-soluble phenolic compounds,flavonoids,amino acids,inorganic elements in Cibotium barometz was summarized,and pharmacological effects of osteoporosis treatment,stanch bleeding,easing pain,bacteriostasis,anti-inflammation,anti-rheumatism of C.barometz was discribed.The information summarized here is intended to provide a rational foundation for the futher development and utilization of C.barometz in China.关键词：Cibotium barometz;chemical constituents;pharmacological activities
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- 1293
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- 41
发布时间：2024-09-23 - 摘要：Objective: Using the method of statistical analysis to explore the relationship between the Traditional Chinese Medicine formulae of different proportions and the treatment for diseases.Method:We retrieved database containing 2 632 formulae of Radix Angelicae Sinensis and Radix Astragali from the "Dictionary of Traditional Chinese Medicine Formulae",then built the database to analyze the relationship among the ratio,the corresponding major disease and the disease category.Result:The ratio"1∶ 1"appears to be the most（49.43%） in the 2 632 formulae containing Radix Angelicae Sinensis and Radix Astragali;which were most-frequently used for the treatment of the diseases in internal medicine（47.83%） and gynecology（23.18%）.Conclusion: Using the method of the statistical frequency analysis,we can find the applied pattern of the TCM formulae and provide a theoretical basis for using herbs effectively in clinic.
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- 727
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- 26
发布时间：2024-09-23 - 摘要：目的:通过实验教学方法的改革,提高学生学习中医诊断学的主动性,深刻理解脏腑辨证各证的概念、表现和证候分析,学会脏腑辨证的基本方法。方法:在教师指导下,学生亲自进行动物模型的建造,并进行病例分析。结果:通过学生亲自进行动物模型的建造,使学生对中医证候的病因、病位、症状、病机等有深刻的认识,提高了脏腑辨证和证候分析的能力,显著提高了教学效果。关键词：中医诊断学实验;动物模型建造;脏腑辨证;教学方法
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- 104
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- 1
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