最新刊期
卷 17 ， 期 19 ， 2011
- 摘要：Objective: To study on the change of content and intrinsic viscosity from water extract of Gardeniae Fructus by ultrafiltration. Method: Different relatively density was determined by Ubbelohde viscometer. Investigated the change of intrinsic viscosity by dealed with ultrafiltration of different retention molecular weight,then study on the effect of deacetyl-asperulosidic acid methyl ester,genipin-1-β-D -gentiobioside and geniposide by ultrafiltration. Result: With the pore size decreased and relative density increased,the loss rate of components have a tendency to increase;Relative viscosity of extract from Gardeniae Fructus reduced significantly after ultrafiltration, with the membrane pore size and extract the relative density decreased, the relative viscosity of ultrafiltrate also decreased. Conclusion: Viscosity of water extract from Gardeniae Fructus decreased significantly after ultrafiltration.关键词：ultrafiltration;Gardeniae Fructus;intrinsic viscosity
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发布时间：2024-01-04 - 摘要：Objective: To study the application in classification of MCC by principal component analysis(PCA)and cluster analysis method. Method: Determined powder property and other physical properties of 12 kinds of PH101 and PH102 MCC types which obtained from 5 different manufacturers,then PCA and cluster analysis method were used to investigate the classification of MCC. Result: The same result was obtained from PCA and cluster analysis method,it revealed that the basis on classification of PH101 and PH102 MCC types by PCA. Conclusion: PCA and cluster analysis method can be applied to study the classification of MCC with the result was the same as classification of MCC in traditional production application.关键词：MCC;physical property;PCA;cluster analysis;classification study
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发布时间：2024-01-04 - 摘要：Objective: To investigate the technology condition of dextroborneol from Cinnamomum camphora leaves in Yunnan by supercritical CO2 fluid extraction. Method: The content of dextroborneol was determined by gas chromatography(GC), to analysis the conditions of pressure, temperature, CO2 flow rate by orthogonal test and analysis extraction time by single factor test. Result: The optimum extraction condition was as follows:extraction pressure was 30 MPa, extraction temperature was 45 ℃,CO2 flow rate was 20 L·h-1,extraction time was 90 min. Conclusion: The optimum extraction technology had high extraction yield,and it was a extraction and separation method which had considerable potential for development.关键词：supercritical CO2 fluid extraction(SFE-CO2);Cinnamomum camphora;dextro borneol
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction process of polysaccharide from sugarcane leaves by orthogonal design. Method: The yield of polysaccharide as index to optimize extraction process of polysaccharide from sugarcane leaves by orthogonal test with spectrophotometry.The content of polysaccharide was determined by phenol-sulfuric acid method. Result: The optimum extraction technology was as follows:extracted 3 times at 100 ℃ with 30 times amount of deionized water for 5 h per time. Conclusion: The verification test showed that the optimum extraction process was stable,reasonable and feasible.关键词：sugarcane leaves;polysaccharide;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To determine the optimum moulding process of Tongzhi Surunjiang dropping pills. Method: Single experiment and orthogonal design were used to optimize temperature of drug liquid,temperature of cooling,internal and external diameter of emitter, dropping distance and dropping rate.Roundness,hardness,resolving time and pill weight difference were used as criteria. Result: The optimum moulding process was as follow:PEG4000-drug was 1.5 ∶1, internal and external diameter of emitter were 2.5 mm and 3.0 mm, dimethyl silicone oil was coolant, temperature of cooling liquid was 10 ℃,temperature of drug liquid was 90 ℃,dropping rate was 40 drops·min-1, dropping distance was 6 cm. Conclusion: This process is stable and feasible.关键词：Tongzhi Surunjiang dropping pills;moulding process;polyethylene glycol;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To investigate the preparation technology of albumin microspheres with total saponins from Panax notoginseng as raw material. Method: Microspheres were prepared by thermal curing method, optimized preparation process by orthogonal test,investigated the amount of albumin, time of phacoemulsification, temperature and time of curing and so on, morphological study by optical microscope and electron scanning microscope, determinate entrapment efficiency by HPLC. Result: the amount of albumin was 300 mg, time of phacoemulsification was 15 min, temperature of curing was at 105 ℃, time of curing was 20 min. Conclusion: It is feasible that prepare albumin microspheres with panax notoginseng saponins as raw material.关键词：total saponins from Panax notoginseng;albumin microspheres;orthogonal test;preparation technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize the technology of Banxia Baizhu Tianma decoction by SBE with uniform design. Method: Using uniform design table UL(91×33)to optimize the technology conditions of Banxia baizhu tianma decoction by SBE with composite evaluate indexes of gastrodin, glycyrrhetinic acid, total area of HPLC, total flavonoids and the extract of molecular≤1 000. Result: The suitable pH for three times of water solution shoud be 2.00,6.56,8.73 respectively and the total time was 4.916 2 h. Conclusion: Combined with practical production, the optimun pH was 2.0,6.5,8.5, the extraction time was 2.0,1.5,1.5 h respectively.关键词：Banxia Baizhu Tianma decoction;semi-bionic extraction method;uniform design;molecular ≤1 000 extract;comprehensive evaluation
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发布时间：2024-01-04 - 摘要：Objective: To optimize complex purification technology of effective parts from Astragali Radix. Method: The technology of polysaccharide alcohol precipitation and removing protein were optimized by orthogonal test with the yield and content of polysaccharide in the extract as index. Purification technology of total saponins from Astragali Radix was optimized by single factor test. Result: Optimum ethanol deposition technology was as follows:3 times of 95% ethanol was added to the solution and keep still in 18 hours at 40 ℃ with the concentration of 1 ∶1(material:solution volume);The optimum technology of polysaccharide removing protein was as follows:one third volume of Sevage reagent was added into polysaccharide solution with the concentration of 15 g·L-1;Total saponin purification optimum technology was as follows:D101 macroporous resin was used,the ratio of resin to drug was 5 ∶2,eluted with 4 BV of water followed with 4 BV of 70% ethanol in D101 macroporous resin,collected ethanol eluate, evaporated. Conclusion: The polysaccharide and total saponins can be purified with the systemic method and it can be used of the full use of the material and industrial work.关键词：Astragali Radix;polysaccharide;total saponins;orthogonal test;synthetical mark
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发布时间：2024-01-04 - 摘要：Objective: To investigate the optimum extraction technology of bergenin from Yinxian Tongfeibao granules. Method: The optimum extraction process was investigated by L9(34)orthogonal design with the content of bergenin as index.The chief factors were ethanol concentration,the amount of ethanol,flow rate of percolation fluid which influencing extraction efficiency. Result: The optimum extraction technology was as follows:percolated with 60% ethanol,collected 10 times(to raw material)percolation fluid, flow rate of percolation was 5 mL·min-1·kg-1. Conclusion: The optimum extraction technology is scientific,reasonable and feasible.关键词：bergenin;HPLC;extraction process;orthogonal design
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发布时间：2024-01-04 - 摘要：Objective: To optimize the extraction process of Ru'an gel cream. Method: The optimum extraction process was selected by orthogonal test.The content of icariin,total flavones from Epimedh Folium and tetrahydropalmatine as indexes. Result: Extraction process of Epimedh Folium was as follows:reflux extract 3 times with 15 times of water,1 h per time. Extraction process of Corydalis Rhizoma was as follows:reflux extract 3 times with 15 times of 50% ethanol,1.5 h per time. The transfer rate of icariin,total flavones from Epimedh Folium and tetrahydropalmatine were 62.64%、83.83%and 93.27%. Conclusion: The optimum extraction technology is resource-saving,stable and practicable.关键词：Ru’an gel cream;icariin;tetrahydropalmatine;extraction process
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发布时间：2024-01-04 - 摘要：Objective: To optimize ethanol extraction process of Jutongning patch. Method: Optimized concentration of ethanol extract are investigated by direct comparison with paeoniflorin,total alkaloids and the amount of alcohol extracts as index.Optimized ethanol extraction processes of Paeoniae Radix Alba,Coptidis Rhizoma,Corydalis Rhizoma from Jutongning patch are investigated by using orthogonal design.Paeoniflorin and the amount of alcohol extracts were used as index in the investigation. Result: Paeoniae Radix Alba,Coptidis Rhizoma,Corydalis Rhizoma were ground to coarse powder and extracted twice in 70% ethanol with 2 h per time,10 times ethanol for the first time and 8 times ethanol for the second time. Conclusion: The optimum process are rational and has high yield of active constituents.关键词：Jutongning patch;paeoniflorin;total alkaloids;the amount of alcohol extracts;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To optimize the best extraction technology of Hanbi patch. Method: Orthogonal test was used to optimize alcohol extraction process of Aconiti Kusnezoffii Radix with the yield of total alkaloid as index;Optimize decocting technology of Paeoniae Radix Rubra and other five herbs by orthogonal design with the extraction amount of paeoniflorin as index. Result: The optimum extraction technology was as follows:Aconiti Kusnezoffii Radix was refluxed with 6 times amount of 70% ethanol for two times by 2 h per time. Paeoniae Radix Rubra and other herbs was added 8 times amount of water for three times with 1 h each time. Conclusion: The optimized extraction process is reasonable,feasible and provide scientific basis for the production.关键词：Hanbi patch;extraction technology;orthogonal test;total alkaloid;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective: To optimize alcohol extraction process of Anshen granules. Method: The optimum alcohol extraction conditions were investigated by orthogonal design with the content of jujuboside A and schizandrin as indexes.Determined by HPLC, using Lichrospher C18column(4.6 mm×250 mm,5 μm),column temperature was 30 ℃,flow rate was 1.0 mL·min-1;Determined jujuboside A with CH3CN-water(30.5 ∶69.5)as mobile phase, the UV detection wavelength was 204 nm;Schizandrin were investigated with methanol-water(13 ∶7)as mobile phase, the UV detection wavelength was 250 nm. Result: The amount of ethanol and extracting time had significant effect on extraction of schizandrin.The optimum alcohol extraction technology of Schisandrae Chinensis Fructus and Ziziphi Spinosae Semen from complex prescription was as follows:extracted 2 times with 10 times amount of 85% ethanol for 1.0 h per time. Conclusion: This extraction technology is reasonable,simple and suitable for mass production.关键词：jujuboside A;schisandrin;extraction process;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of microwave drying technology in the production of concentrated pill. Method: Three varieties pills were tested by microwave drying process,compared technology data with oven drying. Result: The character of products, time-limit of dissolve, moisture, the limit of microorganism were all accord with the regulation of standard. Microwave drying was more effective. Conclusion: Microwave drying had characteristics of uniform heating, drying time was short, improved drug quality and so on.It can be widely used in the production of concentrated pill.关键词：microwave;drying;concentrated pill
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发布时间：2024-01-04 - 摘要：Objective: To study on preparation and drug release behavior in vitro of medicine loaded polyvinyl alcohol(PVA)fibrous membrane. Method: The micro-fibers of 2-SeCD/PVA were prepared by electrospun technique, whose relative weight percentage was 10%, 20%, 30% and 40% respectively. The structure and property of medicine loaded micro-fibers with different weight percentage were characterized by FT-IR and SEM. Drug release behavior in vitro of medicine loaded micro-fibers were investigated by ultraviolet-visible spectrometer in artificial intestinal fluid and artificial gastric juice. Result: The diameter of medicine loaded fibers became larger with increasing 2-SeCD content, however, the grown of accumulative released medicine curve was slower.The cumulative drug release percentage was higher in the simulated intestinal fluid than in the gastric juice with the same volumn percentage of 2-SeCD/PVA micro-fibers. Conclusion: Medicine loaded micro-fibers had better effective sustained-release and certain targeted drug delivery.关键词：electrospinning;medicine loaded fibers;polyvinyl alcohol;sustained-release
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发布时间：2024-01-04 - 摘要：Objective: To determine the content of Arsenic(As)in radix notoginseng, panaxatriol saponins(PTS)and Sanqi Tongshu capsule. Method: The content of As was determined by hydride generation atomic fluorescence spectrometry. The optimal experimental conditions of microwave digestion-hydride generation were optimized. Result: Fluorescence intensity and concentration was in a good linear relationship;the linear range was 0-12 ng·mL-1and the recovery was in the range of 88.37%-104.76%, the detection limit was 0.109 6 ng·mL-1, RSD 0.82%;the content of As in PTS and its preparation were low. Conclusion: The method was simple, rapid and high sensitive. It can be used in the determination of As in radix notoginseng, protoparaxotriol saponins and its preparation.关键词：Notoginseng Radix et Rhizoma;panaxatriol saponins;Sanqi Tongshu capsule;arsenic;atomic fluorescence spectrometry
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发布时间：2024-01-04 - 摘要：Objective: To establish a high performance liquid chromatography method of simultaneously determining 6 constituents in Flos Lonicerae and Jinqi Jiangtang tablets. Method: The separation was carried out on an ODS C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.1% H3PO4 as mobile phase by gradient elution. The flow rate was 1 mL·min-1 and the detection wavelength was at 355 nm. The column temperature was at 30 ℃. Result: The six ingredients were separated well. The linear ranges of chlorogenic acid, caffeic acid, rutin, hyperoside, luteolin-7-O-glucoside and 4,5-O-dicaffeoylquinic acid were 3.24-64.80, 0.24-4.79, 1.22-24.40, 0.09-0.44, 0.68-13.55, 1.19-23.87 mg·L-1 respectively. The average recoveries were above 97% (RSD<3%, n=9). Conclusion: This method is simple and accurate with good recovery and repeatability, and can be used in Jinqi Jiangtang tablets and the related preparation quality control.关键词：HPLC;Jinqi Jiangtang tablets;chlorogenic acid;4,5-O-dicaffeoylquinic acid;rutin;luteolin-7-O-glucoside
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发布时间：2024-01-04 - 摘要：Objective: To establish the quantitative analysis method for borax, boric acid and total boron in Zhengshiming diyanye. Method: Borax, boric acid and total boron in Zhengshiming Diyanye were determined by micro-titration and constant titration. Result: The linear range of borax was from 8-16 mg, and the regression equation was Y=504.52X-0.14( r=0.998 8) with the average recovery rate 98.29%(n=6,RSD 1.37%). The linear range of boric acid was from 50-70 mg, and the regression equation was Y=141.74X+0.07, r=0.999 7 with the average recovery rate 97.02%(n=6,RSD 0.78%). The linear range of total boron was from 0.8-1.2 mmol, and the regression equation was Y=9.51X-0.05, r=0.999 7 with the average recovery rate 95.29%(n=6,RSD 0.75%). Conclusion: This method is simple with high accuracy and easy to popularize, can provide foundation for the quality control of Zhengshiming diyanye.关键词：Zhengshiming diyanye;borax;boric acid;total boron
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发布时间：2024-01-04 - 摘要：Objective: To establish the method for the content determination of paeoniflorin and salvianolic acid B in Huoluoxiaoling granules. Method: HPLC was adopted to determine the content of paeoniflorin and salvianolic acid B. The column for paeoniflorin is Zorbax SB-C18(4.6 mm×150 mm,5 μm). The mobile phase is consisted of acetonitrile-water(10 ∶90)with the detection wavelength at 230 nm. The column temperature was at 40 ℃ and the flow rate was set at 1.0 mL·min-1. The column for salvianolic acid B is Zorbax Extend-C18(4.6 mm×150 mm,5 μm). The mobile phase is consisted of methanol-acetonitrile-0.1% formic acid(22 ∶10 ∶68)with the detection wavelength at 286 nm. The column temperature was at 25 ℃ and the flow rate was set at 1.0 mL·min-1. Result: There was good linearity between the peak area and concentration within the range of 0.084 4 -0.928 4 μg(r=0.999 8)for paeoniflorin and 0.226-2.034 μg(r=0.999 9)for salvianolic acid B respectively. The average recoveries of paeoniflorin and salvianolic acid B were 100.4%(RSD 2.30%)and 100.7%(RSD 2.73%)respectively( n ＝6). Conclusion: The method is simple,accurate and specific for quality control of Huoluoxiaoling granules.关键词：Huoluoxiaoling granules;paeoniflorin;salvianolic acid B;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To compare the content of evodiamine and rutaecarpine from traditional decoction, compound granules and granule formulation in the Zuojin pills . Method: The content of evodiamine and rutaecarpine in the three forms of Zuojin pills was determined and the method to determine content was established by HPLC. Result: There is 15.455 3 mg·g-1 evodiamine and rutaecarpine in the traditional decoction, 21.792 4 mg·g-1 in the compound granules, 26.648 3 mg·g-1 in the formulation granules. Conclusion: There are significant differences in the three forms of Zuojin pills. The order is formulation granules>compound granules>traditional decoction.关键词：HPLC;Zuojin Pills;traditional decoction;compound granules;granule formulation;evodiamine;rutaecarpine
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发布时间：2024-01-04 - 摘要：Objective: To establish the analysis method for content determination of calcium sulfate dihydrate in Xiao’er Qingre Zhike Koufuye. Method: Samples were charcoaled and incinerated to ash, and the content of calcium sulfate dihydrate was determined by complexometric titration. Result: The content of calcium sulphate dihydrate in three batches of Xiao’er Qingre Zhike Koufuye was 1.033, 2.172, 1.872 g·L-1 respectively. The average recovery was 97.35% with RSD of 1.21%. Conclusion: The method established was simple in operation, and can be used for the determination of calcium sulphate dihydrate in Xiao’er Qingre Zhike Koufuye.关键词：gypsum;calcium sulphate dihydrate;Xiao’er Qingre Zhike Koufuye;content determination
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发布时间：2024-01-04 - 摘要：Objective: To study the HPLC fingerprint of hawthorn leaf from different producing areas, establish a method for the analysis of chlorogenic acid, glucosyl-vitexin, vitexin-2″-O-rhamnoside, hyperoside and quercetin. Method: The separation was carried out on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm, 5 μm)with the mixture of acetonitrile and 0.5% formic acid as mobile phase in a gradient elution model(flow rate 1.0 mL·min-1, detection wavelength 350 nm, column temperature 30 ℃). 13 groups of hawthorn leaf were analyzed with the developed HPLC fingerprint and determination methods, the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM(Version 2004A). Result: The linear range of chlorogenic acid, glucosyl-vitexin, vitexin-2″-O-rhamnoside, hyperoside and quercetin were 3.75-120 mg·L-1(r=0.999 6), 9.69-310 mg·L-1(r=0.999 8), 24.69-790 mg·L-1(r=0.999 9), 6.25-200 mg·L-1(r=0.999 9), 0.31-10 mg·L-1(r=0.999 9), respectively. The average recovery rates of chlorogenic acid, glucosyl-vitexin, vitexin-2″-O-rhamnoside, hyperoside and quercetin were 99.2%(RSD 1.7%), 100.3%(RSD 1.8%), 99.0%(RSD 1.4%), 99.8%(RSD 2.1%), 100.5%(RSD 1.9%), respectively. In the fingerprint, 15 common peaks of hawthorn leaf were confirmed and 5 peaks were determined. Conclusion: This method provides a scientific basis for controlling the quality of hawthorn leaf.关键词：hawthorn leaf;HPLC;fingerprint;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for determination of geniposide in Biyuanshu granules. Method: The Kromasil C18 column(4.6 mm×150 mm, 5 μm)was used, and mobile phase was composed of CH3OH-0.2% H3PO4(30 ∶70). Detection wavelength was at 238 nm, the flow rate was 1.0 mL·min-1, column temperature was at 35 ℃. Result: The linear response range was from 0.04-0.51 μg(r=0.999 9).The recovery rate was 100.35%(n=5), RSD was 1.31%. Conclusion: The HPLC method is simple, accurate, and suitable for the determination of geniposide in Biyuanshu granules.关键词：HPLC;Biyuanshu granules;geniposide
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发布时间：2024-01-04 - 摘要：Objective: To optimize and establish a processing technology of Evodia rutaecarpa using stir-bake with ginger on orthogonal test, providing technical index for standardization of stir-bake with ginger on E. rutaecarpa. Method: The content of evodiamine, rutaecarpine and limonin were selected as index;L9(34)orthogonal test was employed to determine the effects of the four factors including quantities of ginger, moistening time, processing temperature and time. Result: Quantities of ginger, processing temperature and time had obviously effects on the content of limonin. The best preparing condition was as follows:the ratio of ginger to raw material was 7.5 to 100, and stir-baked 8 min at the temperature of 160 ℃ after moistening 4 h. Conclusion: The research provided a scientific basis for standardization of processing technology of stir-bake with ginger on E. rutaecarpa using stir-bake with ginger.关键词：orthogonal test methods;processing Evodia rutaecarpa using stir-bake with ginger
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发布时间：2024-01-04 - 摘要：Objective: To assign phenolic acids and determine the content of chlorogenic acid contained in Fructus Xanthii collected from different product areas, and establish a practical quality control method for the herbal drug. Method: Major phenolic acids of this herb were identified based on their UV absorption, retention time and mass fragmentation characteristics. The content of chlorogenic acid was determined using ultra-performance liquid chromatography-ultraviolet detector(UPLC-UV). The mobile phase consists of 0.1% phosphoric acid in water as solvent A and methanol as solvent B. The detection wavelength is set at 327 nm and the column temperature at 35 ℃. The mobile phase flow rate is 0.4 mL·min-1. Result: A total of 9 major chromatographic peaks were assigned as phenolic acids, and peak area vs. content of chlorogenic acid showed a good linearity in the range of 3.5-350 mg·L-1 with a correlation coefficient of 0.999 9 and the average recovery was 101.7%. Conclusion: The established method is suitable for determining the content of chlorogenic acid contained in Fructus Xanthii and can be used to effectively control the quality of the herb;and assigning major characteristic peaks can significantly enhance specificity and accuracy in accessing the quality of herb.关键词：Fructus Xanthii;phenolic acid;identification;determination;UPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a high performance liquid chromatography method for uncertainty analysis of paeoniflorin and albiflorin of TGP. Method: HPLC method was established to determine the content of paeoniflorin and albiflorin. Each active component of uncertainty was calculated the variable parameters among the procedures. The combined uncertainty and extended uncertainty and confidence were finally obtained by synthesized the uncertainties of various component variables. Result: Uncertainty evaluation for paeoniflorin was ±1.48% and albiflorin is ±0.66%. Conclusion: The established mathematic model is reasonable and relible, it is applicable to the uncertainty analysis of high performance liquid chromatography method in the determination of drugs.关键词：high performance liquid chromatography;TGP;paeoniflorin;albiflorin;measurement uncertainty
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for the determination of reference substances in salvianolic acid A. Method: Diamonsil C18 column(4.6 mm×250 mm, 5 μm), acetonitrile-0.2% phosphoric acid solution(30 ∶70)as mobile phase, flow rate 1 mL·min-1, detection wavelength 285 nm, column temperature 30 ℃, other relative substances were controlled by main component self-compare method. Result: The content of salvianolic acid C was less than 1.0%, and the others were less than 3.0%. Conclusion: This method is specific, sensitive, simple, accurate, and can effectively detect salvianolic acid C.关键词：HPLC;main component self-compare method;salvianolic acid C;determination
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发布时间：2024-01-04 - 摘要：Objective: To analyze the volatile components of Feces Trogopterus. Method: The volatile components of Feces Trogopterus were extrabtel by steam distillation and analyzed by GC-MS. Result: Sixty-six components of Feces Trogopterus were Identified and the main components are all-trans-squalene(12.14%),hexadecanoic acid(8.27%),mono(2-ethylhexyl)phthalate(8.25%),dibutyl phthalate(3.80%),tetradecanoic acid(3.04%). Conclusion: The volatile components of the sample are the ingredients with same basic structure and chemical compositions of the foods. The main food of the animal is Rhus leaf at gathering time.关键词：Trogopterus xanthipes;feces;volatile components;Shanyang county of shanxi province;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish a sensitive HPLC-DAD fingerprint method for controlling the quality of compound Tangmaikang. Method: HPLC analysis was performed on a Dikma C18 column(4.6 mm×250 mm, 5 μm), with a mixture of acetonitrile and water as mobile phase in gradient mode. The wavelength was set at 270 nm, and column temperature was 30 ℃. The data were processed by fingerprint similarity evaluation software. Result: A standard HPLC-DAD fingerprint for Compound Tangmaikang with 21 common peaks was established. The similarity of 14 batches of compound Tangmaikang was more than 0.80.Good precision, stability and repetition were showed. Conclusion: This method can be used for the quality control of compound Tangmaikang.关键词：Tangmaikang;HPLC-DAD;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To establish the HPLC fingerprint chromatogram identification of the processed products from Gastrodia elata . Method: HPLC procedure was performed on a chromatographic column of Kromasil C18. Acetonitrile-glacial acetic acid water solution(0.5%)was used as mobile phase by gradient elution, and the detection wavelength was at 270 nm. The mobile phase flow rate was at 1.0 mL·min-1. The similarity and the clustering analysis were carried out by fingerprint chromatogram software. Result: The standard HPLC Fingerprint chromatogram was established through the determination of 10 batches processed products from 6 different producing areas. 15 peaks can be detected and the average similarity was over 98% in 10 samples. The processed products were almost the same on the whole chromatograms but the relative peak areas had the differences. The cluster analysis was consistent with the processed origins. Conclusion: HPLC method can be used in quality control of G. elata .关键词：Gastrodia elata;HPLC fingerprint;identification.
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发布时间：2024-01-04 - 摘要：Objective: To analyze the relationship between the main content of Flos Sophora and heated temperature. Method: 14 portions of Flos Sophora were heated from 120 ℃ to 250 ℃ for 30 min, according to internal temperature was 10 ℃, separately. The content of rutin, quercetin in the heated samples were determined by HPLC. Result: The content of rutin and quercetin were not change singnicantly from 120 ℃ to 160 ℃. The content of rutin was reduced significantly from 170 ℃ to 190 ℃ and the content of quercetin was increased significantly. When heated over 200 ℃, the contents of the two compounds were all reduced. Conclusion: When the heated temperature between 170 ℃ to 190 ℃, the content of main compounds in Flos Sophora changed significantly. When heated over 200 ℃, the main compounds were all damaged.关键词：Flos Sophora;quercetin;heated temperature
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发布时间：2024-01-04 - 摘要：Objective: To study the quality control of Jianpi Kang’er tablets. Method: The content of main component, berberine hydrochlorde was determined by HPLC method. Panax ginseng, Rhizoma Coptidis, Pericarpium Citri Reticulatae were identified by TLC. Result: The negative comparison displayed no disturbance. The linearity of the method founded was within the range of 0.054-0.648 μg(r=0.999 6). The average recovery rate was 97.5% and RSD was 1.51%(n=6). Conclusion: The method was available for quality control of Jianpi Kang’er tablets.关键词：Jianpi Kang’er tablets;quality standard;berberine hydrochlorde;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of volatile oils from Curcuma kwangsiensis to provide the theoretical evidence for controlling its quality. Method: The volatile oils were extracted by supercritical fluid method and analyzed by gas chromatography-mass spectrometry. The relative content of the separated components was determined by normalization method in area. Result: As many as 23 of compounds were identified, containing 96.83%of volatile oils. Conclusion: The main chemical constituents of the volatile oil from C. kwangsiensis are trans-ethyl-p-methoxycinnamate(55.29%), 3,4-dimethoxycinnamic acid(8.57%), anethole(6.29%)and ethyl cinnamate(5.23%).关键词：C urcuma kwangsiensis;volatile oils;supercritical CO2 extraction;gas chromatography-mass spectrometry
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发布时间：2024-01-04 - 摘要：Objective: To study the HPLC fingerprint of Lonicerae Japonicae Flos and establish a chromatographic standard for the quality control as well as to comparative study the HPLC fingerprint among various farm cultivars. Method: The HPLC method was used. The gradient elution was adopted acetonitrile and 1% acetic acid.The 'similarity evaluation system for chromatographic fingerprint of TCM’ algorithm was adopted in combination with the clustering analysis to study ten flos lonicerae’s thirteen characteristic common peaks, and to evaluate the quality and dividide the farm cultivars of flos lonicerae. Result: The HPLC fingerprint of Lonicerae Japonicae Flos were established and the differences of HPLC fingerprint among the farm cultivars of Lonicerae Japonicae Flos produced in Shandong were compared. Conclusion: The HPLC fingerprint method summaried in this article can be used as the typical chromatography and provide conference evidence for dividing the Cultivars resource of Lonicerae Japonicae Flos.关键词：Lonicerae Japonicae Flos;farm cultivars;HPLC;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To isolate and identify chemical constituents from Gentiana algida collected from Qinghai province. Method: The chemical constituents were isolated by column chromatography and their structures were elucidated through NMR, MS and IR. Result: Eleven compounds were isolated and identified as β-sitosterol(1), β-amyrin(2), ursolic acid(3), oleanolic acid(4), 24-hydroxyl- β- amyrin(5), 28-hydroxyl-α-amyrin(6), 28-hydroxyl- β-amyrin(7), pseudotaraxasterol(8), pseudotaraxasterol-3 β- O -palmitate(9), 3 β-palmitate-28-hydroxyl-α-amyrin(10), 3 β-palmitate-28-hydroxyl- β-amyrin(11). Conclusion: All these compounds are obtained from G. algida for the first time.关键词：Gentiana;Gentiana algida;chemical constituents;isolate
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents in the leaves of Psychotria hainanensis. Method: The chemical constituents were isolated by using various column chromatorgaphy and their structures were elucidated on basis of physicochemical constants and spectral data analysis. Result: Seven compounds were obtained and determined to be β-sitosterol(1), stearic acid(2), quercetin(3), rutin(4), kaempferol-7- O -glucopyranoside(5), kaempferol-3- O -rutinoside(6)and daucosterol(7). Conclusion: Compounds 3-7 were obtained from this genus for the first time.关键词：Psychotria hainanensis;chemical constituents;structure elucidate
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Rumex nepalensis . Method: Isolation and purification were carried out repeatedly by silica gel, sephadex gel and ODS C18 column chromatography. And structure identification of compounds was carried out by physical, chemical methods and spectral data. Result: Sixteen compounds were obtained form the petroleum ether and ethyl acetate extraction parts of 80% ethanol extract of R. nepalensis , and identified as β-sitosterol(1), daucosterol(2), hexadecanoic acid(3), hexadecanoic-2,3-dihydroxypropyleste(4), gallic acid(5), ethyl gallate(6), physcion(7), physcion-8- O-β-D -glucopyranoside(8), chrysophanol(9), chrysophanol-8 -O-β-D -glucopyranoside(10), emodin(11), emodin-8- O-β-D -glucopyranoside(12), kaempferol(13), kaempferol-3- O-α-L -rhamnopyranoside(14), quercetin(15)and quercitroside(16). Conclusion: Compounds 2, 4, 5, 6, 8, 13, 14 and 16 were obtained from R. nepalensis for the first time.关键词：Rumex nepalensis;chemical constituent;extraction and isolation;structure identification
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发布时间：2024-01-04 - 摘要：Objective: To discuss the characteristic of the contents of inorganic elements in samples of Dioscorea nipponica and the soil in which the crude drugs were planted, and explore the correlation between them. Method: The contents of 13 elements in ten D. nipponica samples and ten soil samples were determined by means of ICP-MS. The results were provided for calculate the correlations by SPSS. Result: There were no significant correlations between the crude drug and its relevant soil. The most conspicuous accumulation of elements was K, the others were Ni, Hg, Mg, Zn, Cu, Cd, subsequently. Specially, the sample of Jiamusi, Heilongjiang province had the strongest ability to accumulate every element that it can do. And there were multi-correlations among some elements in the crude drug. Conclusion: The majority of pathway of inorganic element absorption are selective absorption in D. nipponica . The coordination among elements is in favor of the accumulation of elements in D. nipponica .关键词：Dioscorea nipponica;soil;inorganic element;correlation
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of the free radical scavenging activity of these formulae for blood-activating and stasis-removing such as Xuefu Zhuyu Decoction(XF), Shaofu Zhuyu Decoction(SF), Gexia Zhuyu Decoction(GX), Shentong Zhuyu Decoction(ST)and Tongqiao Huoxue Decoction(TQ). Method: Free radical scavenging activity was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH)method. Result: The result showed that all these formulae had significant effects on scavenging DPPH radical(P<0.01), in a dose-dependent manner.. The free radical scavenging capacity on DPPH were in the following descending order:GX>TQ>SF>ST>XF. The scavenging ability in total formula was better than in ethanol precipitant and supernatant. Conclusion: Validation studies proved that the enzyme-linked immunosorbent assay for DPPH had good reproducibility and feasibility. The method could be used as high throughput screening for Chinese herbs and traditional chinese medicine formulae.关键词：formulae for blood-activating and stasis-removing;antioxidant activity;DPPH;dose-response relationship
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发布时间：2024-01-04 - 摘要：Objective: To study effects of ethanol extraction of Tripterygium wilfordii Hook. F.(TWH)on cell proliferation, cell cycle and apoptosis in bone marrow cells(BMCs) in vitro . Method: BMCs were cultured in vitro treated with different concentrations of ethanol extraction of TWH. MTT assay was used to evaluate the cell proliferation. The cell cycle and apoptosis rate were determined by FCM with PI and AnnexinⅤ/PI staining. Result: Viability of BMCs was significantly reduced by ethanol extraction of TWH in a time-and dose-dependent manner. Compared with control group, more BMCs were arrested at G0/G1 phase by TWH. Early Apoptosis rate was increased significantly. Conclusion: Ethanol extraction of TWH could notably inhibit the prolifteration of BMCs, arrest BMCs at G0/G1 phase and induce apoptosis of BMCs.关键词：Tripterygium wilfordii;bone marrow cells;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Jiawei Sini powder on learning and memory ability and synaptic plasticity in prefrontal cortex of sleep deprived rats. Method: Experimental model of sleep deprivation was established by use of multi-platform method. The intervention group were fed with Jiawei Sini powder, and the dosage is 16.25 g·kg-1. The variation of learning and memory was detected by Y maze method at pre and post sleep deprivation.The changes of the ultrastructure of rat prefrontal cortex were observed by using transmission electron microscopy. Result: The percentage of correct responses of intervention group was significantly higher than those in model group (P<0.05) .Compared with the model group, the post synaptic density (PSD) in prefrontal cortex areas in the intervention group was significantly thickened (P<0.01) . Conclusion: Jiawei Sini powder could improve the ability of learning and memory in sleep deprived rats via increasing synaptic plasticity in prefrontal cortex.关键词：sleep deprivation;Jiawei Sini Powder;prefrontal cortex;learning and memory;synaptic plasticity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of blood-activating method on brain water content and aquaporin4(AQP4)expression in intracerebral hemorrhage(ICH)rats. Method: SD rats were randomly divided into the following groups:the sham operated group, model group, blood-activating high dose group and blood-activating low dose group. ICH rat model was established by injection autologous blood. The brain water content was determined by wet-dry weight,the expression of AQP4 protein and AQP4mRNA in cerebral tissues were determined respetively by immunohistochemitry techniques and real-time PCR. Result: Compared with those in the sham group, the expression of AQP4 mRNA and AQP4 protein increased significantly in the model group(P<0.01),and the brain water content showed the same trend. Compared with the model group, the expression of AQP4 mRNA and AQP4 protein and the brain water content decreased obviously in blood-activating groups(P<0.01), in a dose dependent manner. Conclusion: Blood-activating and stasis-removing herbs could decrease the edema of ICH, and the probable mechanism might be downregulation of AQP4.关键词：blood-activating and stasis-removing;intracerebral hemorrhage;brain water content;aquaporin
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of curcumol on the expression of apoptosis induce factor(AIF),poly ADP-ribose polymerase(PARP)and caspase-3 protein in lung cancer cell A549. Method: A549 cells were treated with different concentrations(0,12.5, 25, 50,100 mg·L-1)of curcumol for 48 hours, then the total protein was extracted from each group respectively. The expression of AIF,PARP and caspase-3 was detected by Western blotting. Result: The PARP and AIF protein expressions were significantly elevated in the treatment groups(1.661 3 and 2.212 4 respectively)compared with the model group(0.545 3 and 0.358 6 respectively), in a dose dependent manner(P<0.01). The caspase-3 precursor(32×103)protein expression showed no significance difference among all the groups. Conclusion: Curcumol could induce apoptosis through caspase-independent pathway in human lung cancer cell A549, whose mechanism might be linked to up-regulation of the PARP protein and translocation of AIF.关键词：curcumol;lung cancer;apoptosis induce factor;poly ADP-ribose polymerase;caspase-3
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发布时间：2024-01-04 - 摘要：Objective: Effects of platelet aggregation and thrombin time in vitro in rabbits by water extracts and volatile oil from Olibanum and Myrrha and their compatibility were studied to evaluate their effect and dose-effect relationship. Method: Extracts and oils from Olibanum and Myrrha and their combination in different compatibility on platelet aggregation induced by adenosine diphosphate(ADP) in vitro, thrombosis by using thrombin time (TT) method and their dose-effect relationship were observed. Interaction effects of two drugs used in compatibility were measured by an isobole method. Result: The results showed that all the extracts and their combinations in different compatibility had significant or very significant inhibitory effects on the platelet aggregation induced by ADP. Compatibility of the extracts had synergism effect and its activity was better than both single-use of extracts. The experimental data of TT indicated that different extracts from Olibanum and Myrrha could all significantly prolong clotting time of blood plasma in rabbits. Except that combination of water extracts of Olibanum: Myrrh (1 ∶1) had antagonism effect, the others had synergism effect. Conclusion: Water extracts and volatile oil from Olibanum and Myrrha and their combinations in different compatibility all showed significant effect in vitro on platelet aggregation and thrombin time in rabbits. Furthermore, except that compatibility of water extracts of Olibanum: Myrrh (1 ∶1) had antagonism effect, the other showed synergistic action. The study provided reference for scientific characters and rationality of herbal compatibility.关键词：Olibanum;Myrrha;herbal pair;effect;dose-effect relationship
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发布时间：2024-01-04 - 摘要：Objective: To compare the effects of the ethanol and water extract from houttuynia cordata composition on renal lesion in diabetic db/db mice. And the mechanism of Houttuynia cordata composition on preventing the development of diabetic nephropathy(DN)was also investigated. Method: Twenty-four db/m and db/db mouse with 8 weeks of age were divided into four groups according to their treatment ways:group db/m was treated with distilled water, group db/db with distilled water;gruop db/db with ethanol extracted Houttuynia cordata composition;group db/db with water extracted composition. The changes in body weight, 24 h urinary albumin excretion, serum insulin, fasting plasma glucose were measured after mouse were treated for 4 weeks and 8 weeks. Result: After treatment with houttuynia cordata composition for 8 weeks, the plasma glucose, cholesterols and 24 h urinary albumin decreased. Compared with the untreated diabetic controls, both of ethanol and water extracted led to similar amelioration of hyperlipidemia and hyperglycemia, as well as reduction of serum insulin level. Conclusion: The houttuynia cordate composition could improve diabetic nephropathy, and the mechanism might be linked to the reduction of the urinary albumin and improvement of insulin resistance.关键词：houttuynia cordata composition;db/db mice;insulin resistance;diabetic nephropathy
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发布时间：2024-01-04 - 摘要：Objective: To explore antihyperglycemic, antihyperlipidemic, antihypertensive and anti-tumor effects of the cactus fruit polysaccharide extract(CPFP). Method: The diabetic rats were induced by STZ in SD rats, and randomly divided into model group, insulin group(10 U·kg-1·d-1), high-dose group of CPFP(2.37 g·kg-1·d-1), low-dose group of CPFP(1.58 g·kg-1·d-1). The CPFP were administrated daily via gavage for eight weeks. During the experiment, the blood glucose of rats were determined. Male SD rats were fed by hyper-fatty diet for 3 weeks in order to establish the hyperlipemia rat model. The hyperlipemia rats were randomly divided into model group, lovastatin group(3.0 mg·kg-1·d-1), high-dose group of CPFP(2.37 g·kg-1·d-1), low-dose group of CPFP(1.19 g·kg-1·d-1). Different dose of CPFP were administrated daily via gavage for 3 weeks. The blood lipoprotein of rats were determined. Fifty male spontaneous hypertension rats(SHR)at the age of 15 weeks were randomly divided into model group, captopril group(0.01 g·kg-1·d-1), high-dose group of CPFP(2.37 g·kg-1·d-1), middle-dose group of CPFP(1.58 g·kg-1·d-1), low-dose group of CPFP(0.76 g·kg-1·d-1). Ten male Wistar rats were served as normal group. Different dose of CPFP were administrated daily via gavage for 9 weeks. The systolic blood pressure(SBP)of experimental rats was detected weekly. S180-tumor bearing mice were established and divided into model group, cyclophosphamide(CTX)group(0.02 g·kg-1·d-1), high-dose group of CPFP(1.97 g·kg-1·d-1), middle-dose group of CPFP(0.99 g·kg-1·d-1), low-dose group of CPFP(0.49 g·kg-1·d-1). The CPFP were administrated daily via gavage for 10 days. Tumor inhibition rates were recorded. Result: Compare with model group, the content of blood glucose was remarkably decreased and the hypoglycemic rates were 54.08% and 44.73% respectively in high-dose and low-dose group of CPFP. The levels of TC, TG and LDL-C were decreased significantly(P<0.01)in high-dose and low-dose group of CPFP. The level of HDL-C was obviously increased in high dose group of CPFP(P<0.05). The SBP level of SHR in CPFP treatment groups was depressed notability, nevertheless the SBP level of SHR of model group was increased obviously comparing with normal group after 9 weeks. The tumor was inhibited in CPFP treatment groups. The tumor inhibited rates were 49.70%, 31.13 in high-dose, middle-dose group of CPFP respectively after 10 days. Conclusion: CPFP could markedly decrease blood glucose in STZ-induced diabetic rats and decrease blood lipoprotein in hyperlipemia rats. CPFP showed effects on reducing blood pressure in SHR and certain anti-tumor.关键词：cactus fruit polysacharide extract;antihyperglycema;antihyperlipidema;antihypertensive;anti-tumor
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Jiawei Taohe Chengqi decoction(JWTHCQD)on learning and memory in Aβ1-42 induced Alzheimer’s disease(AD)in rats and study its mechanism. Method: Rats were injected bilaterally of aggregated Aβ1-42, Aβ42-1 or PBS into hippocampus mounted using stereotaxic apparatus for AD model establishment. Three groups rats were treated with JWTHCQD(20 g·kg-1)or saline solution respectively. After 21 days, learning and memory ability of the rats were determined by Morris water maze test. And hippocampus tissue specimens for each group were harvested for Tau and p-Tau protein detcted by Western blotting. Result: In place navigation test, the time of escape latency was significantly longer in Aβ1-42 group than that in other groups(P<0.05), but shorter in JWTHCQD treated Aβ1-42 group(P<0.05). Besides, Tau and p-Tau protein were all down-regulation in the Aβ1-42 group after JWTHCQD treatment. Conclusion: JWTHCQD could improve the learning and memory in AD model rats by regulating Tau and p-Tau protein expression.关键词：Jiawei Taohe Chengqi decoction;Alzheimer’ s disease;learning and memory;tau protein;p-Tau protein
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发布时间：2024-01-04 - 摘要：Objective: To investgate the effect of Shuyu granules on monoamine neurotransmitters and inflammatory factors in hippocampus of post-stroke depression(PSD)rats. Method: PSD model was established by permanent bilateral common carotid artery ligation together with a behavior limitation. The spontaneous behavior was observed and monoamine neurotransmitters in the hippocampus and tumor necrosis factor-α(TNF-α)and interleukin 1β(IL-1β)were detected in 22 days after surgery. Result: Compared with the model group, there were significant differences in the behavior ability of PSD rats in three dose groups of Shuyu granules and Fluoxetine group(P<0.05). Compared with the model group, norepinephrine(NE), dopamine(DA),serotonin(5-HT) were significantly increased(P<0.05)in the three dose groups of Shuyu Granules., IL-1β and TNF-α were significantly decreased(P<0.05). Conclusion: Shuyu granuls could increase monoamine neurotransmitters and reduce cytokines and improve the spontaneous behavior in post-stroke depression rats.关键词：post-stroke depression;Shuyu granuls;monoamine neurotransmitters;tumor necrosis factor-α;interleukin 1β
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发布时间：2024-01-04 - 摘要：Objective: To investigate the involvement of reactive oxygen species(ROS)in glaucocalyxin A(GLA)-induced cytotoxicity of human chronic myelogenous leukemia K562 cells. Method: After 12, 24 and 48 hours treatment by GLA,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay was performed to assess the cytotoxicity. The cell cycle was studied by flow cytometry. The ROS burst induced by GLA was also determined by flow cytometry. Result: Exposure of K562 cells to GLA lead to a dose-and time-dependent cytotoxicity with IC50 values of 6.168, 2.968, 1.086 mg·L-1 for 12, 24, 48 h, respectively. The block function of K562 cells cycle after treated GLA mainly occurred on G0/G1 phase. There is a generation of ROS in K562 cell after using GLA. NAC can increase the cell viability and decrease cell cycle arrest. Conclusion: The results suggested that ROS might be involved in the K562 cytotoxicity induced by GLA.关键词：glaucocalyxin A;reactive oxygen species;cytotoxicity;cell cycle
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发布时间：2024-01-04 - 摘要：Objective: To study effects of the total flavones from Mori Folium(TFMF)on myocardial cell apoptosis and caspase-3 expression in myocardial ischemic-reperfusion injury in rats. Method: Ninety SD rats were randomized into sham operation group,model group,three dose groups of TFMF(35, 70, 140 mg·kg-1·d-1),Tonxinluo capsule group(100 mg·kg-1·d-1).The left anterior descending coronary artery was ligated for 30 min, followed by 2 h of reperfusion to establish the rat model of myocardial ischemia-reperfusion injury. myocardia apoptosis were detected by the TUNEL staining and caspase-3 expression was detected by immunohistochemical technique method. Result: IS/LV and IS/AR increased obviously in model group compared with those in sham group, Myocardial apoptosis rate increased obviously(P<0.01)and caspase-3 protein expression was significantly elevated(P<0.05 or P<0.01)in the model group. Compared with model group,IS/LV,IS/AR and myocardial apoptosis rate in TFMF group decreased obviously(P<0.05 or P<0.01),and caspase-3 protein expression was down-regulated(P<0.05 or P<0.01). Conclusion: TFMF possessed an obviouse ameliorate effect on myocardial cell apoptosis in myocardial ischemic-reperfused injury rats by decreasing the caspase-3 protein expression.关键词：total flavonoid of Mori Folium;myocardial ischemia-reperfusion injury;myocardia apoptosis;caspase-3
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Toutongning capsule on P2X3mRNA expression in trigeminal ganglion of migraine rats. Method: The healthy SD rats were evenly divided into:normal group. model group, three dose groups of Toutongning(760, 380, 190 mg·kg-1)capsule group and Xibiling group. Migrained rats model was established by NTG subcutaneous injection. Samples were harvested and measured in 3 hours and 6 hours after NTG subcutaneous injection. The P2X3mRNA expression in trigeminal ganglion was determined by RT-PCR. Result: P2X3mRNA expression in trigeminal ganglion increased in the model group compared with that in the normal group(P<0.01). P2X3mRNA expression was elevated at hour 3 then decreased at hour 6.P2X3mRNA expression in trigeminal ganglion and thalamencephalon were downregulated in the three dose groups of Toutongning capsule and Xibiling group compared with those in the model group(P<0.01). Conclusion: Toutongning capsule could treat migraine by reducing P2X3mRNA expression in trigeminal ganglion.关键词：migraine;Toutongnning capsule;trigeminal ganglion;P2X3mRNA;rat
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shuyu Wan on the immunity of murine AIDS(MAIDS)induced by the Friend murine leukemia virus(FLV). Method: Experimental model was established by inoculated intraperitoneally(ip)with FLV in mice. Meanwhile, treatment groups were intragastric administrated with Shuyu Wan. Three weeks later, blood was withdrawn to assay interleukin-2(IL-2), interferon(IFN)-γ and IL-6 by enzyme linked immunosortent assay(ELISA). Percentage of cluster of differentiation 4+(CD4+)T lymphocyte and CD8+T lymphocyte were determined by flow cytometry. In addition,some mice were injected cyclophosphamide(CTX)and given Shuyu Wan for 5 days, then, leucocytes, hemoglobins, and erythrocytes were dectected. Result: FLV model group showed no evident differences to the treatment groups. But compared with CTX group, the amount of leucocytes, hemoglobins, and erythrocytes were higher markedly in Shuyu Wan groups(P<0.05). Conclusion: Shuyu Wan could not ameliorate immunity of MAIDS induced by the Friend murine leukemia virus. Friend virus infection model might not be appropriate for anti-HIV study by traditional Chinese medicine.关键词：Shuyu Wan;Friend murine leukemia virus;mice;immunity
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发布时间：2024-01-04 - 摘要：Objective: To observe effect of effective constituents of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma on the expression of α-SMA and E-cadherin in UUO rat kidney. Method: Sixty-six SD rats were randomly divided into 7 groups, normal group, model group, fosinopril group, salvianolic acids group, astragalus saponins group, granules combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group, constituents combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group. Each group was treated with different does of drugs after UUO surgery for 14 days. The doesage of fosinopril group was 6.6 mg·kg-1, salvianolic acids group was 40 mg·kg-1, astragalus saponins group was 37.5 mg·kg-1, constituents combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group was given salvianolic acids 40 mg·kg-1 and astragalus saponins group was 37.5 mg·kg-1,granules combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma group was given Astragali Radix granules and Salviae Miltiorrhizae Radix et Rhizoma granules 10 g·kg-1.Blood was withdrawn from abdominal aortic to determine the renal function(creatinine, urea nitrogen)and metabolic cages were used to collect urine for retinol binding protein(RBP)determination, the obstructed kidney was taken out and fixed by 10% paraform and made into paraffin section by dehydration and embedding which were used for immunohistochemistry experiment. Result: The level of serum Cr in UUO rats reached (52.34±7.63) μmol·L-1, was higher than that of (43.29±4.13) μmol·L-1 in the normal group, however, except fosinopril, there was no significant difference between the treatment group and the model group No significant differences were found in the serum BUN. The standard of urinary RBP expression in every UUO group was significantly higher than normal group(P<0.05), compared with the model group that its figure was(0.49±0.09)μmol·L-1,the fosinopril group and constituents group RBP levels were(0.41±0.05),(0.41±0.04)μmol·L-1,of which had obvious significance(P<0.05). Immunohistochemical results showed that the most severely pathological changes was in the model group,the lightest pathological changes were found in fosinopril group and the group with constituents combination of Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma. Conclusion: Renal function has been damaged in UUO rats, Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma effective constituents and their combination could ameliorate renal function and reduce the expression of tubular protein RBP in urine. Astragali Radix and Salviae Miltiorrhizae Radix et Rhizoma and their active constituents intervention could alleviate renal fibrosis at a certain degree.关键词：renal fibrosis;Astragali Radix;Salviae Miltiorrhizae Radix et Rhizoma;α-smooth muscle actin;E-cadherin
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发布时间：2024-01-04 - 摘要：Objective: To observe the protect effects of chlorogenic acid on liver-injury of mice induced by Carbon Tetrachlotide (CCl4). Methods: Animals were devided into 6 groups:normal group, model group, solvent group, the chlorogenic acid high-dose group,middle-dose group,low-dose group. The dose of chlorogenic acid is ig 14,7,3.5 g·kg-1respectively. The mice models of acute chemical liver injury was induced by CCl4 . The indexes of the live and spleen, alanine transaminase(ALT) and aspartate transaminase(AST) activity in serum were examined. Superoxide dismutase(SOD), glutathione peroxidase(GSH-Px)activity,MDA level in liver tissue were determined by immunological liver injury. Meanwhile liver pathological examination was observed by light microscope. Result: The chlorogenic acid (14,7,3.5mg·kg-1)could remarkablly resist the increase of ALT(40.01±7.14),(51.19±9.45), (62.66±9.01)U·L-1,AST (43.59±4.32),(52.99±5.97),(66.97±7.34)U·L-1, decreased the MDA level (21.68±10.28),(28.54±6.84),(35.51±9.87) nmol·g-1, and could improve SOD activity (112.98±8.41),(98.36±11.18),(81.76±9.88) U·g-1and GSH-Px level (196.19±10.56),(169.77±13.61),(141.57±15.11) U·g-1 (P<0.01 versus model group for above results). Conclusion: Chlorogenic acid has remarkable protective effects on liver –injury in mice.关键词：Eucommiae Folium;chlorogenic acid;carbon tetrachlotide (CCl4);liver-injury
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发布时间：2024-01-04 - 摘要：Objective: To observe the assistant effect of EBFL on S180 tumor mice and explore its possible mechanism. Method: Mice tumor model was applicated; the model groups were divided into EBFL-low dose group (20 mg·kg-1), EBFL-high dose group (40 mg·kg-1), and combined group with cyclophosphamide(CTX, 20 mg·kg-1, 40 mg·kg-1) ig for 2 weeks.Life span, the tumor growth inhibition rate, WBC count, and indexes of immunological founctions, lymphocyte transformations,interleukin-2(IL-2) and tummor necrosis factor-α(TNF-α) levels were observed. Result: Compared with the model group, the EBFL could prolong life span but there was no obvious inhibition effect on S180 tumor. Compared with the CTX group, effect of high dose EBFL group was more obvious and could reverse inhibiting activity of weight, WBC count, immunological function indexes (P<0.05,P<0.01),EBFL could improve IL-2 levels(P<0.05,P<0.01).The effect of high dose EBFL group was more obvious and could reverse the inhibiting activity of lymphocyte transformation(P<0.01). Conclusion: The EBFL can prolong life span, and attenuate the toxicity of CTX as well as adjust the immunity function.关键词：extraction of buckwheat flower and leaf;cyclophosphamide;immunity;S180 tumor
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发布时间：2024-01-04 - 摘要：Objective: to observe protection of total flavonoids in anti-coxsackie B complex capsule(K-CoxB-JN) against acute myocardial injury caused by adriamycin in mice and to discuss the feasible mechanism. Method: One hundred and seventy five Kunming mice were selected and diveded into 7 groups, including high dose group of flavonoid (0.8 g·kg-1), middle dose group of flavonoid(0.4 g·kg-1), low dose group of flavonoid, model group(0.2 g·kg-1), Xinxuekang group, blank group and original formula group., there 25 mice in each group are taken. After a week, model of acute myocardial injury was established by adriamycin injection of 20 mg·kg-1. Blood was collected after 24 h of model building, then determination of myocardial enzyme like SOD and MDA were performed as well as indexes of heart and spleen. Result: Activity of myocardial enzymes was decreased drastically with total flavonoids in K-CoxB-JN, as well as indexes of heart and spleen, especially middle dose group of flavonoid. Conlusion: Total flavonoids in K-CoxB-JN do prectect mice against acute myocardial injury. The possible meachanism may be related to antioxidation and scavenging of oxygen free radicals.关键词：anti-coxsackie B complex capsule;total flavonoids;adriamycin;myocardial injury
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-fatigue effects of ginseng and its processed products. Method: One hydred and twenty mice were allocated randomly into ginseng, red ginseng and red ginseng processed by high pressure groups. Each treatment was divided into three subgroups by dosages(5,2.5,1.25 g·kg-1). Blank control group was treated with 0.9%NaCl. Drugs were administered to mice for 15 days, then fatigue-related biochemical indicators were analyzed. Result: Compared with the control group, the mice of ginseng group had a higher content of liver glycogen(11.33±3.45)g·kg-1(P<0.01)and muscle glycogen(1.98±0.83)g·kg-1(P<0.05), and lower content of blood urea nitrogen(BUN)(29.71±6.42)g·L-1(P<0.05);the mice of red ginseng group had a higher content of liver glycogen(10.46±3.21)g·kg-1(P<0.01), and lower content of BUN(29.45±5.06)g·L-1(P<0.05);the mice of high pressure red ginseng group had a higher content of liver glycogen(9.87±2.44)g·kg-1(P<0.01). Conclusion: All the three types of ginseng showed anti-fatigue effects.关键词：anti-fatigue;Ginseng;Red ginseng;red ginseng processed by high pressure
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发布时间：2024-01-04 - 摘要：Objective: To investigate the sedative and hypnotic effects of Tianwang Buxin Wan(TWBXW)on mice model. Method: ICR rats were divided into five groups randomly, three dose groups of TWBXW(3.24,1.62,0.81 g·kg-1),control group and Annao Jianshen group. Spontancous locomotor activities of mice in five minutes, precentage of sleep, sleep latency, maintaining sleep were evaluated.The effects of TWBXW on spontaneous locomotor activities in normal mice group and yin deficiency mice group were observed. Hypnotic effects were assayed by cooperated with pentobarbital sodium. Result: There was no direct influence of TWBXW on the spontaneous locomotor activity in normal mice. But it could significantly decrease the spontaneous locomotor activity of mice with yin deficiency syndrome. TWBXW could reduce sleep latency and extend sleep time induced by pentobarbital sodium. Conclusion: TWBXW showed certain sedative and hypnotic effects.关键词：Tianwang Buxin Wan;mice;sedation;hypnogensis
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-inflammatory effect of Jianghuang Xiaocuo Chaji. Method: Anti-inflammatory effect of Jianghuang Xiaocuo Chaji was studied on various experimental models. Result: Jianghuang Xiaocuo Chaji (1.35,0.90,0.45 mg·kg-1, externally used three times daily for 4 d) inhibited mice paw edema produced by ovalbum and reduced the production of prostaglandin-2(PGE2) in inflammatory tissues. In allergic arthritis mice, Jianghuang Xiaocuo Chaji (1.35,0.90,0.45 mg·kg-1 externally used twice daily for 30 d) could inhibit the dermal pathological changes and decrease the content of serum interleukin-2(IL-2). Conclusion: Jianghuang Xiaocuo Chaji showed remarkable antiinflammatory effects, reduced the production of PGE2 and decreased the content of serum IL-2.
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects of Ligustrazine combined with puerarin on the changes of neurological deficits. Method: Rats were randomized into sham-operation group, model group, Ligustrazine-puerarin group(ligustrazine 50 mg·kg-1-puerarin 50 mg·kg-1)and nimodipine group(0.5 mg·kg-1). The model was established by 4 vessel occlusion method. The changes in nerve function and brain morphology were evaluated. Result: After ischemia reperfusion, the model group neurologic deficit and morphological changes were higher than that in the sham-operation group significantly (P<0.01).Neuronal damage and inflammatory reactions could be found in the model group(the sections stained with Nissle staining). In Ligustrazine-puerarin group and nimodipine group, the neurologic deficit scores and morphological changes were lower than that in the model group (P<0.05, P <0.01). Conclusion: Ligustrazine-puerarin can prevent and alleviate the ischemia and reperfusion injury to some degree.关键词：ligustrazine;puerarin;compatibility;cerebral ischemia;rat;nerve function;morphology
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Tianzhicao capsule on tumor cell cycle. Method: S180 tumor-bearing mice were administraed with Tianzhicao capsule orally(0.5,1.0,2.0 g·kg-1, 7 d). Cyclophaspharmide, the positive control, was given at a dose of 0.02 g·kg-1·d-1. The changes of tumor cell cycle after administration were detected by flow cytometer. Result: Tianzhicao capsule could blockade the course of tumor cell cycle. The rate of Sub-G1 and G0/G1 phase of Tianzhicao high dose group were increased from (2.26±0.11)% up to (2.61±0.04)% and from (47.34±0.70)% up to (55.23±1.05)% accordingly. And the rate of S phase was decreased. Conclusion: Tianzhicao capsule can arrest tumor cell cycle in G0/G1 phase and inhibit proliferation of tumor cell.关键词：Tianzhicao capsule;tumor cell cycle;G0/G1 phase;S phase
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of total flavones from Cuscuta chinensis (TFCC)on the rat osteoblasts metabolism in vitro . Method: Improved tissue block culture was used. Osteoblast from newly born SD rats was used. MTT, PNPP, RIA and ELISA were used separately to observe the proliferation, the activity of alkaline phosphatase(ALP), secretion of osteocalcin (BGP) and Collagen Type I(COL-I) of osteoblasts cultured in vitro with TFCC of different concentration (0.01, 0.1, 1, 10, 100 μg·L-1) in it for different incubation periods. Result: After 48 h and 72 h, 5%TFCC had the effect on stimulating cell proliferation and activity of ALP; and TFCC promoted secretion of BGP and COL-I after 48 h of cultured osteoblast. Conclusion: TFCC can significantly promote the proliferation and the differentiation, and may influence the bone metabolism by promoting the secretion of COL-I.
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Zhisheng Capsule on the rat model of esophageal cancer, inducing apoptosis and the impact on Fas, FasL protein expression. Method: Healthy Wistar rats were treated with MANA 5 mg·kg-1 for 18 weeks except normal group.After the last injection,the rats were randomly divided into the following groups:esophageal model group, Zhisheng Capsule high(0.75 g·kg-1), middle (0.5 g·kg-1),low (0.25 g·kg-1)dose groups, tegafur group (20 mg·kg-1). TUNEL assay was used to examine the effect of Zhisheng Capsule on apoptosis of esophageal cancer cells. Immunohisto-chemistry (IHC) technique was used to measure the expression of Fas and FasL protein in cells. Result: Zhisheng Capsule of three doses could promote apoptosis of esophageal cancer cells according to the result of TUNEL assay. Comparison with model group was significant (P<0.05). High dose group was significantly better than chemotherapy group and low dose group (P<0.05). There showed some dose-effect relationship. Measured with IHC, the expression of Fas protein in the cells dealt with Zhisheng Capsule was increased, whereas the expression of FasL protein was decreased . Conclusion: Zhisheng Capsule could promote apoptosis of esophageal cancer cells. The molecular mechanism of Zhisheng Capsule may be related with up-regulating of Fas and down-regulating the expression of FasL. In addition, the effects of Zhisheng Capsule to promote apoptosis of esophageal cancer cells and Fas, FasL showed some dose-effect relationship. The effect of the capsule was better than tegafur.关键词：Zhisheng Capsule;esophageal carcinoma;apoptosis;fas;fasL
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Shexiang Wulong pill on pathomorphology and expression of vascular endothelial growth factor (VEGF) and endostatin (ES) of synovium tissue in the rats with adjuvant arthritis (AA), and investigate Shexiang Wulong pill treatment for the rheumatoid arthritis whether concerns with the adjustment to the imbalance of VEGF/ES. Method: The rats with adjuvant arthritis model was established. Shexiang Wulong pill fills pill low dose and high dose (1, 2 g·kg-1) were given orally. One week later, the structure changes of synovium tissue were observed, and the expression of VEGF and ES in synovium tissue was determined by immunohistochemical test. Result: The Shexiang Wulong pill low, high dose group both could reduce the pathological synovium integral, both dose group compared with model group had difference (P<0.05); the low dose group had no statistical difference with the high dose group. Compared with the model group, the low and high dose groups also could reduce the expression of VEGF, increase the expression of ES and reduce the ratio of VEGF/ES in synovium tissue(P<0.05 or P<0.01). The low dose group had no statistical difference with the high dose group. Conclusion: The Shexiang Wulong pill can attenuate the degree of chronic inflammation and angiogenesis in synovium of AA rats. The mechanism might be associated with the adjustment to the imbalance of VEGF/ES in synovium of AA rats.关键词：Shexiang Wulong pill;rheumatoid arthritis;adjuvant arthritis;vascular endothelial growth factor;endostatin
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发布时间：2024-01-04 - 摘要：Objective: To explore effects of tanshinone ⅡA on expression changes of nephrin and transforming growth factor-β1(TGF-β1)in adriamycin(ADR)-induced-nephropathy rats. Method: Fifty-six rats of Sprague-Dawley were divided into two groups:the model group(n=44)and the control group(n=12).The rats in the model group were injected with a single dose of ADR(0.007 g·kg-1)via tail-vein, while the rats in the control group were injected with a comparable volume of 0.9% saline. The model was successfully established when the 24 h urinary protein excretion exceeded 100 mg after ADR injection 1 week, forty rats were successful and then were randomly assigned to the ADR nephrosis group(n=10), prednisone group(n=10), the low dose and high dose of tanshinone ⅡA group(n=10). The rats in the prednisone group were treated with prednisone(0.01 g·kg-1·d-1), ig, The rats in the low dose and high dose of tanshinone ⅡA group were treated with tanshinone ⅡA(0.02 g·kg-1·d-1 or 0.04 g·kg-1·d-1), ig, once a day for 4 weeks,whereas the other rats were received a comparable volume of 0.9% saline. The blood and urina biochemical indicators were measured and the pathological changes of the renal tissues were observed under light microscope, immunohistochemical and Western blotting techniques to detect the expression of nephrin and TGF-β1. Result: The 24 h urinary protein, cholesterol, triglyceride, blood urea nitrogen, serum creatinine, and the expression of TGF-β1 in the ADR nephrosis group, prednisone group, the low dose and high dose of tanshinone ⅡA group were higher than those in the control group(P<0.05). The total protein, albumin and the expression of nephrin in the ADR nephrosis group, prednisone group,the low dose and high dose of tanshinone ⅡA group was lower than those in the control group(P<0.05). After treatment with prednisone and the high dose of tanshinone ⅡA could improve above-mentioned indicators. Conclusion: Nephrin and TGF-β1 play an important role in the pathogenesis of ADR nephrosis; tanshinone ⅡA decreased the urine albumin excretory rate, and prevent the glomemlar sclerosis in rats with ADR nephrosis, which mechanism may be at least partly correlated with enhancing the expression of nephrin and inhibiting the expression of TGF-β1 in kidney.关键词：adriamycin nephrosis;proteinuria;nephrin;transforming growth factor-β1;Tanshinone ⅡA
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发布时间：2024-01-04 - 摘要：Objective: To explore the protective effect and mechanism of Qiju Dihuang Wan (QJDH) on kidney in diabetic rats. Method: Rat diabetic model was set up by intraperitoneal injection of streptozotocin. Thirty-two SD rats were randomly divided into normal group, model group, QJDH high and low dose group (2,1 g·kg-1), and each group contained eight rats. The daily treatments were started at 72 h after model establishment, and lasted for 4 weeks.The general state of rats was observed. Blood sugar, kindey weight/body weight index, 24 h urine protein, serum creatinine(SCr), blood urea nitrogen(BUN), malondialdehyde (MDA), superoxide dismutase (SOD) were measured by different methods. Result: QJDH was able to obviously ameliorate the general state of diabetic rats, reduce 24 h urine protein, SCr and BUN content, decrease MDA content and increase SOD activity in kindey tissue. Conclusion: QJDH can relieve the oxidative stress of kindey tissue, and lighten kindey injury induced by diabetes mellitus in diabetic rats.关键词：Qiju Dihuang Wan;diabetes mellitus;kindey function;oxidative stress
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发布时间：2024-01-04 - 摘要：Objective: To research the regulation of Jiangfang Baoxin capsule to vascular endothelial cell function disorder in essential hypertension patients. Method: Sixty-two essential hypertension patients were rando mLy divided into two groups:control group(30 cases)and herb intervention group(32 cases), which were treated with standard therapy and additional Jiangfang Baoxin capsule(orally ter daily,3 capsules each time)respectively for 3 months. The changes of blood pressure and levels of nitric oxide(NO), nitric oxide synthase(NOS), inducible nitric oxide synthase(iNOS), endothelin(ET-1)and angiotention II(AngII)from peripheral blood of the two group patients were compared. Result: After treatment, NO level and NO/ET-1 ratio of herb intervention group was significantly increased than before(P<0.05), while ET-1 dose significantly decreased(P<0.05), and the levels of NOS, iNOS, ET-1 and AngⅡ had no difference;In control group, there was no difference of the values of NO, ET-1, AngII and NO/ET-1 ratio between before and after treatment, and the levels of NOS and iNOS were significantly decreased through the treatment(both P <0.01);After treatment, values of NO, NOS, iNOS and NO/ET-1 ratio of herb intervention were all significantly higher than those of control group(P<0.01). Conclusion: Jiangfangbaoxin capsule could correct (vascular endothelial cell, VEC) function obstacle and protect VEC function of essential hypertension patients.关键词：Jiangfang Baoxin capsule;essential hypertension;vascular endothelial cell function
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of the Shuxuening injection and Trimetazidine on coronary heart disease(CHD)and angina pectoris. Method: One hundred and twenty patients were randomly divided into treatment group and control group. The control group were given routine western medicine,and the treatment group received Shuxuening injection and trimetazidine additionally. 4 weeks was a course of treatment. Result: The total effective rate was 85.00% in control group and 98.33% in treatment group on the clinical symptoms(P＜0.05). The total effective rate was 60.00% in control group and 85.00% in treatment group on the electrocardiogram(P＜0.05).The total cholesterol (TC), low density lipoprotein (LDL-C) and triglyceride (TG) of the two groups was significantly lowered, and high density lipoprotein (HDL-C) was highered,and improving in the treatment group was more significant than in the control group(P<0.05). Conclusion: Shuxuening injection and Trimetazidine has significant therapeutic effect in treating CHD and angina pectoris.关键词：Shuxuening injection;trimetazidine;coronary heart disease;angina pectoris.
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发布时间：2024-01-04 - 摘要：Objective: To observe the changes of small intestines movement and motilin, substance P(SP) levels of rats with functional dyspepsia, to discuss the mechanism. Method: The animal model of functional dyspepsia was established by irregular feeding and stimulating tails of the rats. Forty-eight Wistar rats were randomized into control group, madinglin group, high,middle and low dose groups of shuweifang and modle group, 8 rats in each group. The content of motilin and substance P in plasma were determined by radiommunoassay. And advance rate of small intestines were observed with phenolsulfonphthalein. Result: Compared to the control group, the contents of Motilin(MTL), SP and advance rate of small intestines in model group were obviously decreased(P<0.05), while in the Shuweifang and Madinglin group increased significantly(P<0.05). Conclusion: Shuweifang can increase the contents of MTL and SP in the plasma of model rats and promote advance rate of small intestines which is probably one of the mechanisms in the treatment of functional dyspepsia.关键词：Shuweifang;functional dyspepsia;advance rate of small intestines;motilin;substance P
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发布时间：2024-01-04 - 摘要：Objective: Observe clinical efficacy of the traditional prescription of Xuanfu Daizhe Tang curing chronic pharyngitis. Method: Xuanfu Daizhe Tang(Flos Inulae,Rhizoma Ginseng,Rhizoma Zingiberis,Rhizoma Glycyrrhizae,Rhizoma Pinelliae, Fructus Gardeniae)treat chronic pharyngitis. Result: The total effective rate is 69.1%. The effective rate of female is 62.5.6% and of male is 73.4%. Conclusion: This prescription has obvious effection to chronic pharyngitis significantly. The efficacy of male patients was significantly higher than female.关键词：chronic pharyngitis;Xuanfu Daizhe Tang;phlegm in the coke
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Ziyin Jianghuo Fang(ZYJHF) on patients suffering from menopausal syndrom (MPS) caused by deficiency of yin and exess of fire,try to explore its potential mechanism. Method: Sixty cases with MPS caused by deficiency of yin and exess of fire were randomly divided into two groups. The therapy group(30) was treated by ZYJHF, the control group(30) was treated by placebo A course lasted for 4 weeks, and total treatment was 3 courses. Result: The main symptom:flush, sweating in the therapy group was relieved(P<0.05),while in the control group the symptom was not relieved. In the two groups, the Kupperman scores of and traditional Chinese medicine(TCM) symptom were decreased significantly(P<0.05), but with significant difference in the two groups(P<0.01). Conclusion: ZYJHF could relief the clinical symptoms of MPS, particularly effective on hectic fever and perspiration, it can improve the life quality of patients suffering from MPS caused by deficiency of yin and exess of fire.关键词：menopausal syndrom;syndrome of deficiency of yin and exess of fire;Ziyin Jianghuo Fang;hectic fever;perspiration;integration of Kupperman;integration of syndrome
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发布时间：2024-01-04 - 摘要：Objective: To clarify the research progress in the mechanism of regulating vascular smooth muscle cell apoptosis by Chinese materia medica. Method: Looked through the related literatures from domestic and abroad recent years, analyzed and reviewed on them. Result: Many kinds of Chinese materia medica can regulate vascular smooth muscle cell from different molecular level to regulate its abnormal apoptosis, thus to reverse or change the pathologic state accordingly. Conclusion: Chinese materia medica can regulate vascular smooth muscle cell apoptosis reasonably, and the molecular biology research of its mechanism has wide perspective.关键词：Chinese materia medica;vascular smooth muscle cell;apoptosis;mechanism
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发布时间：2024-01-04 - 摘要：Literatures aboutpreventing vascularcomplication of diabetes mellitus(DM)have beenanalyzed. There aremore and more DM patients with DM vascular complications, which has been paid attention for its big damage.Traditional Chinese medicine shows its characteristics and advantages in DM treatment. Its applicationisgrowing concern. This articlemainlyreviews the traditional Chinese medicine(tablets, injection, decoctions), single herb withextracts(pieplant, coix seed, ect.), and monomer components(salvianolic acid, puerarin, geniposide, naringin and astragaloside)in the prevention and treatment of vascular complications of DM(diabetic nephropathy and diabetic retinopathy), including clinical observations, animal studies in vivo and in vitro experiments.Aiming to explore the advantages of Chinese medicine in the treatmentof DM,andimprove itsapplicationin theresearch of DM and new drug development.
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发布时间：2024-01-04 - 摘要：To introduce the principles of response surface optimization method, design method and its application in Chinese traditional medicine research and development,based on the extensive literature, describing the concept of response surface methodology to the principle, design method, the stpes in the extraction of traditional Chinese medicine, and prescription of traditional Chinese medicine preparation process of screening applications and research in the future development of Chinese medicine. so response surface optimization method with a good prediction, experimental simplicity, high efficiency and other characteristics, can be a good application in pharmaceutical research.关键词：response surface method;method of designing;steps;design of Chinese traditional medicine experimental
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