最新刊期
卷 18 ， 期 6 ， 2012
- 摘要：Objective: To study on effect of azone on in vitro transdermal absorption of puerarin from Guping cataplasmata. Method: Transdermal absorption of puerarin was investigated by Franz diffusion pool method when used by azone alone or combination of azone and other penetration enhancers. Result: Transdermal absorption of puerarin could be enhanced by different concentration of azone, especially when azone combined with camphol and volatile oil in this prescription. Conclusion: Added 5% azone in Guping cataplasmata could remarkably increase absorption rate and absorbing capacity of puerarin.关键词：Guping Cataplasmata;Azone;puerarin;transdermal absorption
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发布时间：2024-01-04 - 摘要：Objective: To optimize vinegar technology of Pueraria lobata. Method: By taking the content of puerarin, daidzin and daidzein as indexes, three factors of the amount of vinegar, moistening time, slow fire frying time were investigated, optimum vinegar technology of P. lobata was optimized by orthogonal test. Result: Optimum vinegar technology was as follows: took a certain amount of P. lobata, added 20% vinegar to moisten 0.5 h, slow fire frying time was 30 min. Conclusion: This optimized technology was stable, feasible and reproducible.关键词：Pueraria lobata;stir-frying with vinegar;puerarin;daidzin;daidzein
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology for Sishen colon targeting tablets by orthogonal test design. Method: L9(34) orthogonal test was used for optimizing content determination extraction technology for Sishen colon targeting tablets with the concentration of ethanol, the amount of solvent, extraction times and extraction time as investigation factors,extraction rate and extract yield of psoralen, isopsoralen, evodiamine, rutaecarpine was taken as indexes. Result: Optimum extraction technology was as follows: reflux extracted 3 times with 8 times the amount of 60%ethanol for 1.5 h each time. Conclusion: Optimized extraction technology was rational with high extraction efficiency, it was suitable for industrial production.关键词：Sishen colon targeting tablets;orthogonal design test;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To study on stability of active ingredients from Rheum officinale in concentration and drying process. Method: Taking active ingredients from extraction fluid of R. officinale as studying target,samples were taken at different time points in concentration and drying progress,change trends of relative content was determined by HPLC. Result: The relative content of active ingredients were degraded greater degree after concentrated 1 h in process of heating at 70 ℃,its reduction is not obvious after concentrated 2 h by comparing with 1 h,but after concentrated for 3 h,the relative content reduced significantly; At 70 ℃ vacuum drying process,active ingredients were in large degree of degradation after drying 2 h, the change of content was small after drying 3 h by comparing with 2 h,general trend was relative stable,the content of active ingredients was less affected in drying process.Sennoside D decomposed easily in heating. Conclusion: During in process of concentration and drying at 70 ℃ vacuum for extraction fluid from R. officinale , concentration time should be controlled within 2 h,components were stable in drying time for 3 h.关键词：Rheum officinale;concentration and drying;active ingredients;peaks ascription;stability
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发布时间：2024-01-04 - 摘要：Objective: To study on purification technology conditions of total flavonoids from leaves of Ficus carica by macroporous resin. Method: Five models of macroporous resins were selected by static adsorption,purification technology of total flavonoids from leaves of F. carica with macroporous resin was optimized by dynamic adsorption. Result: Optimum purification technology was as follows:concentration of sample 2 g·L-1,velocity of sample 2 BV·h-1, volume of sample 3 BV,eluted with 2 BV deionized water and abandoned eluate, then eluted with 4BV 80% ethanol at speed of 2 BV·h-1,eluate was collected;Yield of total flavonoids was 51.3% at this technology conditions, and recovery rate of total flavonoids was 77.6%. Conclusion: D140 macroporous resin could purify total flavonoids from leaves of F. carica effectively.关键词：leaves of Ficus carica;total flavonoids;macroporous resin;D140;purification
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发布时间：2024-01-04 - 摘要：Objective: To resarch chemical composition change after enrichment and purification of alkaloids from Aconitum carmichaelii with D101 macroporous resin. Method: Taking transfer rate of total alkaloids, diester diterpenoid alkaloids, aconitine,hypaconitine and mesaconitine as indexes, before and after purification,the content of alkaloids from extracts of A. carmichaelii was determined by UV and HPLC,ingredients of alkaloids differentiated by TLC. Result: Alkaloids from A. carmichaelii was enriched by D101 macroporous resin,transfer rate and purity of total alkaloids were 83.70%,67.34%,respectively;Transfer rate of aconitine,hypaconitine and mesaconitine were 77.78%,94.12%,52.63%;It showed 6 similar biological spots by TLC comparison, this indicated that there was no significant difference before and after enrichment of alkaloids composition. Conclusion: D101 type macroporous resin could effectively enhance purity of total alkaloids from A. carmichaelii with high transfer rate of alkaloids,it could be used for production promotion.关键词：Aconitum carmichaelii;total alkaloids;total diester diterpenoid alkaloids;aconitine;hypaconitine;mesaconitine
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for extracting, purifying and preparing isoguanosine from Croton tiglium. Method: Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result: Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol, 40 min each time. After preliminarily purifying by macroporous resin,isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion: This method was simple and easy to operate, also could be used for preprartion high purity isoguanosine samples.关键词：Croton tiglium;isoguanosine;extraction;purification;macroporous resin;preparative liquid chromatography
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发布时间：2024-01-04 - 摘要：Objective: To optimize optimum separation and purification technology of pseudo-ginsenoside F11 from total saponins in leaves and stems of Panax quinquefolium. Method: PF11 was determined by HPLC-ELSD with yield of PF11 as index,and AB-8 type macroporous adsorption resin was choosen to purify PF11from total saponins in leaves and stems of P. quinquefolium. Result: Optimum technology was:total saponins in leaves and stems of P. quinquefolium passed 10 times the amount of AB-8 macroporous adsorption resin with flow rate 5 mL·min-1,eluted with 7.5 BV 30%ethanol-33%ethanol,discard eluate,then eluted with 8 BV 35%ethanol-40%ethanol,collected eluate,recovered ethanol,evaporated, purity of pseudo-ginsenoside F11 was more than 50%. Conclusion: This method of PF11was separated by macroporous adsorption resin was simple,rapid and feasible.关键词：pseudo-ginsenoside F11;macroporous adsorption resin;separation and purification
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method of in vitro release for spray film of extract from Galla Chinensis, and investigate its in vitro release characteristics. Method: With gallic acid as index,modified Franz diffusion cell was chosen with semi-permeable membrane as in vitro permeability barrier, the content of gallic acid was determined by HPLC and in vitro release of gallic acid was calculated. Result: Affect of penetration enhancers on release effect of this spray film was not obvious,equation of in vitro release model fitted best with Kinetic equation. Conclusion: This spray film had characteristics of good release and Kinetic equation, it could be achieved a better release effect without penetration enhancers.关键词：extract from Galla Chinensis;spray film;penetration enhancers;in vitro release
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发布时间：2024-01-04 - 摘要：Objective: To investigate freeze-drying technology of hydrocamptothecin-loaded liposomes (HCPT-lipo). Method: Effect of various types and concentrations of cryoprotectives on HCPT-lipo were investigated with appearance, particle size, disparity and encapsulation efficiency as indexes,and to optimize freeze-drying technology parameters. Result: 1.5% mannitol could prevent liposomes from aggregating better as cryoprotective, optimized process was as follows:precooled at -45 ℃ for 10 h,maintained at -30 ℃ for 8 h,maintained at -10 ℃ for 6 h,maintained at 25 ℃ for 2 h. Conclusion: Stable HCPT-lipo could be obtained by screening cryoprotectives and optimizing freeze-drying process parameters.关键词：long-circulating liposomes;freeze-drying
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发布时间：2024-01-04 - 摘要：Objective: To prepare wogonin albumin microspheres, and evaluate its in vitro release behavior and stability. Method: The microspheres were prepared by emulsion cross-linking method with albumin as carrier.Based on single-factor test,preparation technology of wogonin albumin microspheres were optimized by orthogonal design test,investigated size of microspheres and in vitro release property. Result: Prepared microspheres were round and surface was smooth,the average particle size was (0.95±0.14) μm,the average drug loading was (53.58±3.26)% and the average entrapment efficiency was (62.54±3.17)%,in vitro release was in line with Higuchi equation Q=10.402 8 t1/2-1.205 6 (r=0.998 2). Conclusion: This paper obtained ideal wogonin albumin microspheres,its in vitro release characteristics was in line with property of sustained-release preparation.关键词：wogonin;albumin microspheres;in vitro release
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发布时间：2024-01-04 - 摘要：Objective: To investigate influence of sulfur fumigation on ecdysterone from Achyranthes bidentata. Method: The content changes of ecdysterone after fumigation of sulfur was determined by HPLC,XB-C18 column (4.6 mm×250 mm,5 μm),mobile phase acetonitrile-water (15: 85),detection wavelength 250 nm,column temperature 25 ℃,flow rate 0.8 mL·min-1. Result: Conditions of maximum amount of ecdysterone was:the amount of sulfur 100 g·m-3,fumigation once for 2 h;Linear relationship of ecdysterone in the range of 0.408-2.448 μg was good, the regression equation was Y=34.167X+61(r=0.999 6). Conclusion: Sulfur fumigation of ecdysterone from A. bidentata had a certain influence,the content of ecdysterone had a decreasing trends with increasing the amount of sulfur, prolonging exposure time and increasing fumigation times.关键词：Achyranthes bidentata;sulfur fumigation;ecdysterone;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To prepare fibrauretinum colon targeting tablets delivery system controlled by pH-dependent and time-dependent effects, and to investigate its in vitro release. Method: Fibrauretinum colon targeting tablets were coated sequentially with isolated layer of HPMC and PEG 6 000, time-dependent layer of polyacrylic resin Ⅳ and PVP, and outermost enteric coating layer of polyacrylic resin Ⅱand polyacrylic resin Ⅲ. Release situation of coated tablets in artificial gastrointestinal environment were investigated. Result: There was no drug release of prepared fibrauretinum colon targeting tablets in pH 1.0 artificial gastric juice in 2 hours;In pH 6.8 artificial intesrinal fluid, the amount of release was less than 1% in 3.5 hours;But in pH 7.6 artificial colon fluid, drug was completely released within 6 hours. Conclusion: This study reveals that fibrauretinum colon targeting tablets had a good effect of colon targeting.关键词：fibrauretinum;colon targeting;in vitro release
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation process of β-asarone cataplasm. Method: Orthogonal design test was employed to optimize matrix proportion and molding technology of β-asarone cataplasma with glossiness,adhesiveness,matrix residue,skin following and repeatedly exposing as indexes. Result: Optimum matrix proportion of carbomer-pressure sensitive adhesive-polyvinyl alcohol-sodium carboxymethy cellulose-gelatinum-citric acid-sodium citrate-glycerol-propylene glycol was 0.3:1:0.05:0.35:0.1:0.12:0.18: 3.5:3.5. Optimized molding technology were that stiring speed was 500 r·min-1,refining temperature was 45 ℃,refining time was 15 min,the content of of β-asarone was 8.7%. Conclusion: Optimized β-asarone cataplasm had a good adhesive power,excipients and glossiness,drug loading was large and no skin left.This preparation had a certain advantages and wide application prospects for treatment of stroke.关键词：β-asarone;Cataplasm;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To optimize precipitation desalination technology for regeneration silk fibroin by orthogonal test. Method: Application of neutral calcium chloride prepared regenerated silk fibroin solution,desalination was applied by ethanol precipitation,desalination technology was optimized by orthogonal test with investigating factors(the concentration of ethanol,the amount of ethanol,precipitation time) of affecting precipitation process parameters for fibroin protein protein. Result: Optimum precipitation desalination technology for regeneration silk fibroin was:precipitated 4 h with 6 times the amount of 100%ethanol,at this point the concentration of silk fibroin solution was 0.08 g·mL-1. Precipitation desalination efficiency could reach 97.34%,yield of silk fibroin protein was 77.28%. Conclusion: This methord was stable and feasible, it had high desalination efficiency and could be used in desalination technology of protein.关键词：regenerated silk fibroin;orthogonal test;precipitation desalination
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发布时间：2024-01-04 - 摘要：Objective: To optimize flash-type extraction technology of baicalin from Scutellaria baicalensis by response surface methodology (RSM). Method: With yield of baicalin as index, the content of baicalin was determined by HPLC. Concentration of ethanol,extracting time and liquid-material ratio were selected by single-factor test;Technology parameters were optimized by Box-Benhnken design and response surface analysis statistical method. Result: Optimal extraction technology conditions were as follows:concentration of ethanol 73%,extraction time 102 s, liquid-solid ratio 14: 1 (mL: g),under these optimum conditions,measured value of baicalin was 13.588%,it was in line with theoretical value of 13.592%. Conclusion: Flash-type extraction was proved to be a kind of efficient and rapid method for extraction of baicalin from S. baicalensis.关键词：baicalin;flash-type extraction;response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To investigate affecting factors of geniposide content in Xinshenning tablet. Method: Some controlling points in production process of Xinshenning tablet were selected,the content of geniposide was determined by HPLC and transfer rate of geniposide was calculated. Result: Main affecting factors for the content of geniposide were concentration process and granulation process,temperature of these 2 process was controlled below 75 ℃,the average content of geniposide from three samples was 1.87 mg/tablet,average transfer rate was 67.0%. Conclusion: Geniposide could completely extracted and effectively transferred according to this process,product quality was controlled better.关键词：Xinshenning;geniposide;affecting factor
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of compound Hongjingtian grain. Method: Water extraction technology for main component of compound Hongjingtian grain was optimized by contrast test method and orthogonal test method.Took yield of extract and extraction yield of salidroside as indexes to optimize extraction times,soaking time,water volume,extraction time and other factors. Result: Optimum extraction technology was soaked 0.5 h,extracted 2 times,first time extracted 2 hours with 10 times the amount of water,second time extracted 1 h with 8 times the amount of water. Conclusion: This experiment provided basis for water extraction technology of compound Hongjingtian grain, and this technology was reasonable and simple.关键词：compound Hongjingtian grain;extraction technology;salidroside;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To develop a rapid NIR method for determination of paeoniflorin in paeony root. Method: HPLC was used as a reference method to determine the content of paeoniflorin. Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method. Result: the RMSECV of the model for paeoniflorin was 0.019 7,the correlation coefficients of the calibration models was 0.969 7,the RMSEP was 0.046 1. Conclusion: The NIR method was rapid,simple,and noninvasive and that is applied to determine the content of paeoniflorin in paeony root. It can offer reference to content determination of other Chinese herbs.关键词：near-infrared spectroscopy;Paeoniae Radix Alba;rapid analysis
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发布时间：2024-01-04 - 摘要：Objective: In order to explore a rapid approach to determine dulcitol content in Euonymus fortune. Method: Based on fourier transform infrared spectroscopy (FTIR) and used the result of High Performance Liquid Chromatography (HPLC) as controls, partial least square method (PLS), principal component regression (PCR) and multiple linear regression (MLR) were employed to determine the content of Dulcitol in Euonymus fortune collected from 8 different producing areas quantificationally. Result: The results showed that the forecasted values by those of three regression methods basically tallied with the actual values by HPLC method, there was no significant difference among them(P<0.05) and no system errors; The MLR result was optimal with mean standard error of 0.160, 0.296 and the average relative standard deviationwas of 4.475%, 6.643% respectively when the crude drugs and its methanolic extract of Euonymus fortune were selected to determine the value of dulcitol solely, which were less than PLS and PCR methods. Conclusion: The quantitative correction model has stronger capability of determination, the detection on real-time was implemented rapidly with the Fisher model, proposed the model has more practical value.关键词：Euonymus fortune;dulcitol;FTIR;quantitative analysis
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发布时间：2024-01-04 -
Study on Quality Standard for Shangshizhitong Plaster based on Hot-Melt Pressure Sensitive Adhesives
摘要：Objective: To establish the quality control standard of Shangshizhitong plaster based on hot-melt pressure sensitive adhesives. Method: Belladonna Liquid Extract in Shangshizhitong plaster was identified by TLC. The content of brucine and strychnine in Shangshizhitong plaster was identified by HPLC. Result: Atropine in belladonna liquid extract could be detected by TLC. Strychnine shows a good linear relationship in the range of 0.53-53.0mg·L-1(r=0.999 5), which average recovery was 95.57% and RSD was 2.91% (n=6). Brucine shows a good linear relationship in the range of 0.53-53.0mg·L-1(r=0.999 5), whose average recovery was 97.95% and RSD was 1.43% (n=6). Conclusion: The method was simple, specific and accurate. This study provide a method for the quality control of Shangshizhitong plaster based on hot-melt pressure Sensitive Adhesives.关键词：Shangshizhitong plaster based on hot-melt pressure sensitive adhesives;atropine;brucine;strychnine- 2
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发布时间：2024-01-04 - 摘要：Objective: To determine norswertianolin of Gentianella acuta by reverse-phase HPLC method. Method: The determination of norswertianolin was performed on a shim-pack VP-ODS-C18 column (4.6 mm ×150 mm, 5 μm). The mobil phase was 0.1% phosphoric acid solution (A)-acetonitrile (B) (80: 20). The flow rate was 1.0 mL·min-1. Ultraviolet absorption detector was set at 254 nm and column temperature at 25 ℃. Norswertianolin of G. acuta was separated well. Result: The result indicated that the linear range of norswertianolin of G. acuta was between 9.6-192 mg·L-1(r=0.999 5); the relative standard deviations of instrument precision was 0.67%(n=6); the relative standard deviations of method reproducibility was 1.88% (n=6); the relative standard deviations for stability was 1.68%; the average recovery was 99.8% (n=6). Conclusion: The results indicated that this developed assay method was rapid, accurate, and reliable and could be readily used as a quality control method for the norswertianolin.关键词：HPLC;Gentianella acuta;xanthone;determination
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发布时间：2024-01-04 - 摘要：Objective: To compare the contents of licorice glucoside and glycyrrhizic acid in Sini Decoction processed by traditional extraction method, water extraction and alcohol precipitation method included in Pharmacopoeia and the single herb separately extracted then combined method. Method: To determine the the contents of licorice glucoside and glycyrrhizic acid was determined by three extraction method.Separation was carried out on C18 column. A linear solvent gradient elution is used and solvent A was acetonitrile, and solvent B is water containing 0.05% phosphoric acid. The flow rate was 1.0 mL·min-1 at 25 ℃ and the detector was set at 237 nm. Result: The content of licorice glucoside was 2.57 mg·g-1 in the traditional decoction, 2.21 mg·g-1 in the mixture and 3.58 mg·g-1 in the single herb formula combined solution in a prescription.The content of glycyrrhizic acid was 1.96 mg·g-1 in the traditional decoction, 1.84 mg·g-1in the mixture and 3.04 mg·g-1 in the single herb formula combined solution in a prescription. Conclusion: The contents of licorice glucoside and glycyrrhizic acid in the single herb formula combined solution in a prescription is higher than the others. The contents of licorice glucoside and glycyrrhizic acid is significantly different in the preparation processed by different extraction methods which can provide references for Sini decoction extraction technology.关键词：Sini Decoction;licorice glucoside;glycyrrhizic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a microwave-assisted extraction method for the extraction of the naringin from Drynaria fortunei. and compare of the determination of naringin from D. fortunei between microwave-assisted extraction and Chinese pharmacopeia method. Method: Dikma Diamonsil C18 column(4.6 mm×150 mm,5 μm)was used. The mobile phase consisted of methanol-acetic acid-water (35: 4: 65). The temperature of column was mainted at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 283 nm. Result: Microwave extractive time was 5 min,the temperature was 120 ℃,the extraction solvent was 50%methanol. The calibration curve was in good linearity with in the range from 0.04 to 0.6 μg(r=0.999 9). The average recovery was 99.51% (n=6). Conclusion: The method is simple,rapid,as well as precise and reliable,and can be used for the determination of naringin from D. fortunei.关键词：microwave-assisted extraction;Drynaria fortunei;naringin;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish a determination method of the paridis saponinsⅠand Ⅱ for the content comparison of ethanol-extraction and granule of paridis. Method: Kromasil C18column(4.6 mm×250 mm,5 μm)was used.Acetonitrile-water(42: 58)was used as a mobile phase.Flow rate was 1 mL·min-1.Detection wavelength was at 210 nm,column temperature was in room temperature. Result: The Paridis saponinsⅠconcentration and peak area was showed good linear relationship between 0.0435-0.87 g·L-1(r=0.999 1).Paridis saponins Ⅱ concentration and peak area was showed good linear relationship between 0.038-0.76 g·L-1(r=0.999 7).recovery of Paridis saponinsⅠand saponins Ⅱ was 99.90% and 100.2% separately.RSD is 1.6% and 1.34%,the content of paridis saponin Ⅰand Ⅱ in ethanol-extraction and granule is 34.7% and 3.1% respectively. Conclusion: This method is simple with short ratention time and well separation,the content of saponin Ⅰand Ⅱ in ethanol-extraction were significantly higher than that of granule.关键词：HPLC;paridis saponinsⅠ;paridis saponinsⅡ;content determination
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of Rheumatism ostalgia SprayⅠ. Method: TLC was used in qualitative identification of Gelsemium elegans and Zanthoxylum nitidum. And HPLC was used for assay of koumine in the preparation. Result: G. elegans and Z nitidum were detected by TLC. Koumine had a good linear relationship within the range of 0.199 2-3.187 2 μg (r=0.999 7). The average recovery was 98.41%,and the RSD was 1.409%. Conclusion: The method established is stable and reliable. It can be used for quality control of the preparation.关键词：Rheumatism ostalgia sprayⅠ;TLC;HPLC;koumine
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of the gallic gallic acid and ellagic ellagic acid indifferent part of Dimocarpus longan. Method: An HPLC method was performed on a SinoChrom ODS-BP (4.6 mm×250 mm,5 μm) at 30 ℃. The mobile phase was acetonitrile-0.1% phosphoric acid with gradient elution and changeable wavelength detection(0-11.5 min,215 nm for gallic acid and 11.5-25 min,255 nm for ellagic acid).The flow rate was 0.8 mL·min-1 and the sample size was 10 μL. Result: The calibration curve of gallic gallic acid and ellagic ellagic acid showed good linearity over the range of 0.229-2.29 μg(r=0.999 9),0.412 5-4.125 μg(r＝0.999 9).The average recoveries were 98.27%(RSD 0.63%),98.31%(RSD 0.88%). Conclusion: This method is sensitive,accurate,reproducible, it can be used for the quality control of longan.关键词：longan;gallic acid;ellagic acid;HPLC;variable wavelength
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents from the flowers of Cirsium setosum. Method: Compounds were isolated by chromatographic techniques. Their structures were elucidated by spectral methods. Result: Nine compounds were identified as pseudotaraxasterol (1), pseudotaraxasteryl acetate (2), β-sitosterol (3), (E)-hexacosyl p-coumarate (4), apigenin (5), isokaempferide (6), β-carotene (7), rutin (8), buddleoside (9). Conclusion: Compounds 2, 4, 6 were isolated from Cirsium setosum for the first time.关键词：Cirsium setosum;Asteraceae;Cirsium;chemical constituent
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Mianyi composite decoction. Method: The constituents were separated and purified by column chromatography on silica gel, RP-silica gel and Sephadex LH20 from 30% alcohol elution on microporous resin chromatograph of Mianyi composite decoction. Their structures were elucidated on the basis of spectra data. Result: Eleven compounds were isolated and identified as 4-hydroxy-2(E)-nonenoic acid (1), adenine (2), quercetin (3), luteolin (4), adenosine (5), scandosidemethyl ester (6), astragaloside Ⅳ (7), galloylpaeoniflorin (8), propanoid acid (9), salvianolic acid C (10), apigenin-7-O-β-D-glucopyranoside (11). Conclusion: All of compounds were isolated from Mianyi composite decoction for the first time, and chemical type of compound 1 was isolated from single herb in Mianyi composite for the first time.关键词：Mianyi composite decoction;chemical constituents;structure elucidation
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发布时间：2024-01-04 - 摘要：Objective: To establish a quantitative HPLC method for the determination of mollugin in extract from Rubia cordifolia L. Method: The determination was carried out on a Ultimate XB-C18 column at 25 ℃ with methanol-acetonitrile-0.2%phosphoric acid (25:50:25) as mobile phase and detection wavelength at 250 nm. The flow rate was 1.0 mL·min-1. Result: The good linearity was obtained with the correlation coefficients ranged of 3.566-456.4 mg·L-1(r=0.999 9). The average recovery rate was 98.97% (RSD 2.29%). Conclusion: This method is accurate and reliable with easy operation, which can be used in the quality control of extracts from R. cordifolia.关键词：Rubia cordifolia L.;mollugin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To measure the content of baicalin in root, stem and leave of Scutellaria baicalensis growing in different habitat. Method: The content of baicalin was determined by HPLC. The chromatographic condition included Kromasil ODS (4.6 mm×250 mm, 5 μm) and mobile phase consisting of a mixture of methanol-H2O (53: 47). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.124-0.93 μg with correlation coefficient 0.999 9. The average recovery of baicalin was 98.17% with RSD 1.3% (n=6). Result: The average content of baicalin in different habitat root was 6.97%, 6.01%, 5.49%, 3.50% and 4.38% respectively. In different habitat stem, the average content of baicalin was 0.10%, 0.08%, 0.09%, 0.06% and 0.03% respectively. In different habitat leave, the average content of baicalin was 0.27%, 0.26%, 0.15%, 0.14% and 0.09% respectively. Conclusion: The method of distill is simple and stable. The determination method was quick with good reappearance. Produced in the same area, the content of baicalin of S. baicalensis in different habitat and different organ had significant different.关键词：Scutellaria baicalensis;habitat;HPLC;baicalin;content
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of different dry methods on ferulic acid in different parts of Ligusticum chuanxiong. Method: The content of ferulic acid in L. chuanxiong from different dry methods and different parts was determined by HPLC. Result: There was a good linear relationship between ferulic acid and peak area when the sample injection was from 0.005 74-0.918 4 μg (r=0.999 9); the quantification limit was 0.114 8 mg·L-1; the average recovery was 100.7%, and RSD was 1.07% (n=9). The ferulic acid content in different parts of L. chuanxiong arranged in descending order was: rhizome>stem>leaf. Different dry methods had different influence on the ferulic acid content in different parts of L. chuanxiong. Conclusion: Ferulic acid content in different parts of L. chuanxiong is different. Sunshine is not suitable for rhizome, oven-drying after sunshine is suitable for L. chuanxiong.关键词：dry methods;Ligusticum chuanxiong;ferulic acid;content determination
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发布时间：2024-01-04 - 摘要：Objective: Select the appropriate reference standard, the establishment of an accurate determination of total flavonoid content. Method: Select rutin as reference substance use spectrophotometry to compare different drying on the actinides chinesis. Result: A variety drying method of actinides chinensis impact on total flavonoids influenced. The content of flavionoids in actinides Chinensis driey by drying, vacuum drying, microwave drying is 10.62, 15.64, 13.32 mg·g-1, respectively. Conclusion: Actinides chinensis was suitable for vacuum drying.关键词：Actinides chinensis;flavonoids;determination;rutin
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发布时间：2024-01-04 - 摘要：Objective: To establish a simple determination method of polysaccharide in Rhizoma Polygonatii, which is not influenced by the content of monosaccharide. Method: The total sugar was determined by phenol-sulfuric acid method while the concentration of reducing sugar was determined by 3,5-dinitrosalicylic acid (DNS) method, the contentration of polysaccharide=Ct(the concentration of total sugar)-Cs(the concentration of reducing sugar). Result: It was used to evaluate the content of polysaccharide collected in different area, the concentrations of polysaccharide in Polygonatum Rhizoma collected fromYunnan province are relatively higher. Conclusion: The results showed above methods are convenient, stable and accurate,which can be used for quality evaluation of Polygonatum Rhizoma and its preparations.关键词：3,5-dinitrosalicylic acid method;phenol-sulfuric acid method;Polygonatii Rhizoma;determination of polysaccharide
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activity Psammosilene tunicoides in vitro. Method: Antioxidant activities of P. tunicoides were evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, diamonium salt (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP) assay. Results were compared with that of positive controls PG, BHA and BHT. Result: Antioxidant activity of three extracts from P. tunicoides was weaker than that of the positive control PG, BHA and BHA. In the three extracts, Antioxidant activities of ethyl acetate extract was good. ABTS radical scavenging (IC50=40.54 mg·L-1) and ferric reducing antioxidant power 2.42) μmol·g-1] of ethyl acetate extract were strong, followed by the ABTS radical scavenging (IC50=83.38 mg·L-1) and ferric reducing antioxidant power·g-1] of n-butanol extract, petroleum ether extract was the weakest. Conclusion: Ethyl acetate extract of P. tunicoides had strong antioxidant activity.关键词：Psammosilene tunicoides W. C. Wu et C. Y. Wu;antioxidant activity;DPPH;ABTS;FRAP
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发布时间：2024-01-04 - 摘要：Objective: To bring forward a method of determining 6 preservatives in Shedan chuanbei mixture and figure out the state of quality of preservative in this mixture. Method: A ODS Hypersil C18(4.6 mm×250 mm,5 μm) column was used with menthol-0.025 mol·L-1 sodium phosphate monobasic as the mobile phase under condition of gradient elution. The detection wavelength of benzoic acid was at 228 nm and the detection wavelength of sorbic acid and parahydroxybenzoate esters were at 258 nm. Result: All the 6 preservatives were completely separated, the average recovery was in the range of 99.2%-102.04%. Conclusion: The method can be used to control the quality of Shedan chuanbei mixture, which provides the advantage of quickness, simplicity and repeatability.关键词：Shedan chuanbei mixture;preservative;HPLC
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发布时间：2024-01-04 - 摘要：Objective: Study on HgS content, species and crystal construct of cinnabar. Comparation of cinnabar from its confusable varieties. Method: The cinnabar were identified by macroscopic,microscopic observation,physical and chemical testing. It supplied the bases for identifying the cinnabar. The structure and paticle diameter of cinnabar were investigated with XRD and SEM. HgS contents of cinnabar was determined by volumetric analysis。 Result: Cinnabar from its confusable varieties were identified By difference of macroscopic,microscopic observation,physical and chemical. The cinnabar have two polymorphism(α-HgS and β-HgS). The XRD spectra of HgS nano-powders collected in the different habitats were very consistent with each other. Conclusion: The property of cinnabar have a close relationship with HgS contents and states and crystal shape. It is a main influencing factors of evaluation of cinnabar’ quality.关键词：cinnabar;mercury polysulfide(α-HgS,β-HgS);species;crystal construct;XRD;SEM
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发布时间：2024-01-04 - 摘要：Objective: Four kinds of Chinese medicinal materials (medicated leaven, Jianqu, pinellia rhizome fermented mass, semen sojae praeparatum) processed by fermentation method were studied through the correlative microbial culture, identification and analysis, to confirm the inherent quality and provide reference for using medicinal materials safely and effectively in clinical. Method: Four kinds of culture dishes (blood plate, Sabouraud, Chinese blue and conventional air) were used to make bacterial culture and morphological testing to the above Chinese medicinal materials. Result: The four Chinese medicinal materials were all cultured more miscellaneous bacteria in the conventional air dishes; gram-negative bacilli were cultured in some Chinese blue Petri dishes; while gram-positive bacillus, gram-negative bacillus, gram-positive coccus and fungal spores were cultured in blood plate in varying degrees; in sabouraud medium, only raw medicated leaven and raw Jianqu could culture fungoid which was distinguished as microzyme by morphological identification. Conclusion: Raw medicated leaven and Jianqu cultured more fungoid microzyme which were beneficial bacteria produced by fermentation. But fungoid have not been found in pinellia rhizome fermented mass and semen sojae praeparatum. Therefore, attention should be paid to the process of fermentation processing, so as to further ensure the quality of medicine materials.关键词：fermentation;processing drugs;fungoid;microorganism
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-inflammation and pharmacodynamics of Leigongteng Duogan Pian. Method: The model of adjuvant arthritis in mice was established with complete freund’s adjuvant(CFA) and the changes of interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α) were determined to estimate the pharmacodynamics. Result: The results showed that concentration-time curves after iv leigongteng duogan tablet were fit to a two-compartment model,The t1/2α was 0.230,0.196 h, t1/2βwas 0.283,0.246 h, V1/F was 2.174,1.798 L·kg-1, AUC0-∞was 1.236, 1.578 mg·L-1·h-1. Conclusion: Pharmacodynamics of anti-inflammation conforms to linear two-compartment model and the distribution and elimination are both fast in mouses in vivo.关键词：Leigongteng Duogan Pian;anti-inflammation;adjuvant arthritis;pharmacodynamics
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发布时间：2024-01-04 - 摘要：Objective: To establish a high performance liquid chromatography(HPLC) method for determining the serum concentration of swertiamain in acute liver damage rat and to investigate pharmacokinetics of swertiamain. Method: The model of acute liver damage rat was established by D-galactosamine(D-GIaN) 400 mg·kg-1 intraperitoneal injection. After the rats were administered by DIGEDA-4 decoction, HPLC was used to determine the serum concentration of swertiamain at different times; Winnonlin 5: 1 procedure was used to calculate concentration-time data and pharmacokinetic parameters. Result: The linearity were ranged from 0.15-4.8 μg·L-1 (r=0.998 9) and the average recovery was higher than 80%. Methodology investigation was all complied with the requirements. After oral administration of DIGEDA-4 decoction, the T1/2 was similar, the relationship between dose and AUC showed good linearity. Conclusion: The swertiamain was fitted to be a two-compartment model in acute liver damage rat. The method described in this report was accurate and has high sensitivity and selectivity which can be used to analyze quantitatively the serum concentration of swertiamain of the DIGEDA-4 decoction.关键词：DIGEDA-4 decoction;swertiamain;acute liver damage rat;pharmacokinetics
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发布时间：2024-01-04 - 摘要：Objective: To study the antidepressant effect and mechanism of Yuxinxiao. Method: Mice were randomly divided into five groups as follows:the model group,positive control group,Yuxinxiao groups(320,640,1 280 mg·kg-1),all groups were administered ig for 7 days, 5-hydroxytryptophan(5-HTP)-induced head-twitch and yohimbine-induced lethality tests were used after the last administration to observe the impact of 5-HTP(10 mg·kg-1,iv) induced head twitch behavior and yohimbine-induced(35 mg·kg-1,sc) lethality in mice; rats were randomly divided into control group, model group, sertraline hydrochloride group(10 mg·kg-1),Yuxinxiao groups(220,440,880 mg·kg-1).The chronic unpredictable mild stress model was established by continuous gavage 8 weeks, the sucrose preference degree,open-field behavior and the content of 5-HT,dopamine(DA) and norepinephrine(NE) in hippocampus were tested to evaluate the empact of Yuxinxiao on CUMS models. Result: Yuxinxiao(320,640,1 280 mg·kg-1,ig)ignificantly increased the frequence of 5-HTP(10 mg·kg-1,iv) induced head-twitch in mice after 7 days of continuous administrations while it did not enhance the yohimbine-induced(35 mg·kg-1,sc)lethality in mice. The contents of 5-HT and DA were increased remarkably in hippocampus of the model group treated with high dose of Yuxinxiao(880 mg·kg-1) after 8 weeks of continuous administrations compared with model groups. Conclusion: Yuxinxiao has a clear antidepressant effect with manner of dose-dependent. The mechanism may be related to the system of 5-HT in central nervous system.关键词：Yuxinxiao;antidepressant effect;head-twitch tests in mice;yohimbine-induced lethality tests;chronic unpredictable mild stress models
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Rehmannia glutinosa polysaccharide (RGP) on inducing differentiation of rats bone marrow mesenchymal stem cells(BMSCs) into neuron-like cells in vitro. Method: After characterized cell surface markers by flow cytometry analysis and assessed cell growth curve by MTT, rat BMSCs were experimented as the object. When the third passage cells grew to fusion of more than 80%, the cells were divided into 4 groups such as the control group, the β-mercap toethanol (BME)induction group, the brain derived neurotrophic factor (BDNF) induction group and the (RGP) induction group. Immunocytochemical stain and RT-PCR method were used to detect several neural specific proteins such as nestin, neuronal specific endase(NSE), glial fibrillory acidic protein(GFAP) and the related mRNA genes expression of nestin, βⅢ-tubulin, NSE and GFAP respectively after 6 hours. Result: Rat BMSCs had good vitality which were isolated, identified and cultivated within five generations. The detection of immunocytochemical stain and RT-PCR method show that neural cell markers were not expressed in the control group, but expressed in the other groups. Positive cells rate of nestin and NSE in the RGP induction group(56.74±1.36)%,(73.37±1.27)% respectively was higher than the BME induction group(28.21±2.43)%,(2.31±2.72)% and the BDNF induction group(31.3±1.61)%,(28.87±1.65)%(P<0.05)and positive cells rate of GFAP(20.17±1.27)%lower (P<0.05), but there was no difference between the BME induction group and the BDNF induction group in nestin, NSE and GFAP positive cells rate. Conclusion: The rat bone marrow mesenchymal stem cells could be differentiated into neure-like cells in vitro with the induction of R. glutinosa polysaccharide, mainly inducted to neuron-like cell.关键词：Rehmannia glutinosa polysaccharide;bone marrow mesenchymal stem cells;neuron-like cells;cell induction
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发布时间：2024-01-04 - 摘要：Objective: Using Rhei Radix et Rhizome with tail clamp and senna combined with restraint to establish and evaluat irritable bowel syndrome of hepatic stagnation and spleen deficiency type. Method: The rats were randomly divided into three groups:control group(K),Rhei Radix et Rhizome with tail clamp group(D+J),Sennae Folium combined with restraint group, keeping modeling 7 days.All rats of control group were fed with NaCl 2 mL, Rhei Radix et Rhizome with tail clamp group was fed with 1 g·mL-1Rhei Radix et Rhizome decoction 20 g·kg-1 according to the body weight, Sennae Folium with restraint group F+S was fed with 0.36 g·mL-1 Sennae Fadium decoction 5.76 g·kg-1 according to the body weight.Watching the general behavioral activity, weight chang and the defecation,etc,after modeling evaluating the fecal points and the time of the first fecal after administration. Result: Rhei Radix et Rhizome with tail clamp and Sennae Folium combined with restraint group has the loose stools but control group has not;the weight growth rates of K,D+J,F+S were 30.24%,11.59%,11.32%;the time of the first fecal after administration were 222.91,182.61,162.36 min;the fecal points in 5 hours after modeling were 4.5,7.68,8.71;the fecal points in 24 hours after modeling were 37.14,43.49,45.54.comparing with the control group(K), D+J,F+S group have significant difference in weight chang, the fecal points and the time of the first fecal after administration(P<0.05). Conclusion: Rhei Radix et Rhizome with tail clamp and Sennae Folumn combined with restraint group can establish irritable bowel syndrome of hepatic stagnation and spleen deficiency type successfully.关键词：hepatic stagnation and spleen deficiency;Rhei Radix et Rhizome;Sennae Folium;tail clamp;restraint
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发布时间：2024-01-04 - 摘要：Objective: To study the mechanism of Tongxie Yaofang (TXYF) in rat model of diarrhea-predominant irritable bowel syndrome (D-IBS). Method: Forty male Wistar rats were divided randomly into normal group,model group, TXYF group, antagonism group. The rat D-IBS model was established by binding stress for 18 d, after D-IBS model was established suceesful, TXYF group and TXYF+antagonism group were orally given TXYF at a dose of 23.6 g·kg-1,once a day for 7 d, meanwhile antagonism group was iv given vasoactine intrestinal peptide(VIP) antagonist at a dose of 35 μg·kg-1 for once a day 4 d after 4 d of being given TXYF. The water content in rat feces was determined. The method of reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry was used to determine the expression of aquaporin-8(AQP8) in colon tissue. Result: ① The water content in rat feces, compared with normal group, in TXYF group was higer(P<0.01),and compared with model group,in TXYF group the water content was significantly reduced (P<0.01), but in antagonism group no change was found. ②The colon expression level of AQP8, compared with model group, the expression of AQP8 in TXYF group was significantly increased (P<0.01), but in antagonism group no change was observed. Conclusion: The mechanism of TXYF in treatment of D-IBS may be related to regulating the secretion of VIP and up-regulating the AQP8 expression in colon tissue.关键词：Tongxie Yaofan;diarrhea irritable bowel syndrome;vasoatine intrestinal peptide antagonist;aquaporin8
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发布时间：2024-01-04 - 摘要：Objective: The present study is designed to observe the protective effects of baicalein against hepatotoxins-induced cytotoxicity on the human normal liver L-02 cells. Method: L-02 cells were seeded and pretreated with or without baicalein (1, 10, 25, 50, 100 μmol·L-1) for 15 min, and then cells were further treated with or without various hepatotoxins, including acetaminophen (AP, 10 mmol·L-1), clivorine (CLI, 50 μmol·L-1), toosendanin (TSN, 2 μmol·L-1) and ethanol (EtOH, 100 μmol·L-1) for 48 h. The survival cells were determined by MTT assay. Result: Four hepatotoxins could significantly reduce the cell viability (P< 0.001) compared with the control group. After treatments, baicalein could increase the cell viability which was decreased by AP, TSN and EtOH (P<0.01,P<0.001). Moreover, baicalein could significantly increase the cell viability which was decreased by CLI (P<0.05,P<0.001). Conclusion: Baicalein can prevent the cytotoxicity induced by those hepatotoxins in human normal liver L-02 cells.关键词：baicalein;hepatotoxicity;hepatocyte
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-tumor action of total saponins from the crude and processed products of Entada phaseoloides in vivo. Method: Mice bearing transplanted tumor S180 were used to observe the effects of total saponins from E. phaseoloides;then inhibition ratio,thymus index,spleen index and liver index were calculated. The 1eve1 of superoxide dismutase (SOD) and malondialdehyde (MDA) was detected. Result: The crude and processed products E. phaseoloides saponins showed a significant anti-cancer activity on S180 implanted in mice, increased the level of SOD and reduced the content of MDA in blood serum in tumor-bearing mice. Conclution: The crude and processed products E. phaseoloides saponins have anti-tumor effects and anti oxidative capacity.关键词：Entada phaseoloides;total saponins;anti-tumor;processing
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influence of Biyuanshu (BYS) nuclear factor-kappa B(NF- κB) p65 expression of nasal sinuses mucosa epithelial in rabbit chronic rhinosinusitis(CRS) model, and explore its possible molecular mechanism. Method: One hundred New Zealand rabbits were randomly divided into normal group, model group, sham operation group, BYS group, western medicine group, with 20 in each group, and CRS model was established. BYS group was given BYS 4.05 g·kg-1·d-1, western medicine group given clarithromycin 25 mg·kg-1·d-1 for 14 days. Nasal sinuses mucosa tissue was collected to observe nasal sinuses mucosa pathological changes with light microscopy after HE dyeing, and detect nasal sinuses mucosal epithelium cytoplasm and nucleus NF-κB p65 protein expression with Western Blotting. Result: Model group appeared chronic inflammation and inflammatory cell infiltration, epithelial cells and glandular organs and goblet cells were hyperplasia obviously. Nasal sinuses mucosal epithelium cytoplasm and nucleus NF-κB p65 protein expression was higher than normal group(P<0.01). After the treatment with BYS, the nasal sinuses mucosa epithele was improved, inflammatory cells and glandular organs and goblet cells were not hyperplasia obviously. Nasal sinuses mucosal epithelium cytoplasm NF-κB p65 protein expression was higher than normal group and sham operation group and western medicine group obviously(P<0.01), nasal sinuses mucosal epithelium nucleus NF-κB p65 protein expression was lower than model group obviously(P<0.01), but there were no differences among nomal group and sham operation group and western medicine group. Conclusion: NF-κB may take part in the development of CRS, BYS and suppress transposing of NF-κB p65 to cell nucleus.关键词：Biyuanshu;chronic rhinosinusitis;mucosa epithelial;nuclear factor-kappa B p65
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发布时间：2024-01-04 - 摘要：Objective: To investigate the influence and possible anti-atherosclerotic mechanism.of serum contained Buyang Huanwu decoction on the expression of toll-like receptor 4(TLR4) and its downstream signal transduction pathway and LOX-1 in human umbilical vein enddhelial cells(HUVECs). Method: Twenty New Zealand rabbits were divided into 4 groups in random:the normal control group, the high dosage group,the middle dosage group, and the low dosage group of Buyang Huanwu decoction, with 5 rabbits in each group. Each group was ig given normal saline and Buyang Huanwu decoction at dosage of 13.2,6.6, 3.3 g·kg-1, respectively everyday. In the 7th day, blood was collected from lefe ventricle in 2 h after administration,and then the serum was separated. HUVECs was cultured in vitro and stimulated with lipopolysaccharide(LPS) 1 mg·L-1 for 2 h, then treated separately with the serum contained different concentrations of Buyang Huanwu decoction for 24 h, finally the expression of TLR4, MyD88, TRAF-6, TRAM,TRIF,NF-κB and LOX-1 mRNA was measured with real-time PCR. Western blotting method was used to analyze the expression of TLR4, MyD88, TRAF-6 and LOX-1 protein. Result: LPS enhanced the expression of TLR4, MyD88, TRAF-6, TRAM,TRIF,NF-κB and LOX-1(vs normal control group, P<0.01). The serum contained Buyang Huanwu decoction decreased the high expression of TLR4, MyD88, TRAF-6,NF-κB and LOX-1 (vs model group, P<0.05),which was stimulated by LPS. Conclusion: The serum contained Buyang Huanwu decoction can block the high expression of TLR4 and its downstream signal transduction pathway and the high expression of LOX-1, which may be the mechanism of anti-artherosclerosis and inhibition of inflammatory reaction.关键词：drug-serum;human umbilical vein endothelial cells;toll-like receptor-4;MyD88;LOX-1;artherosclerosis;Buyang Huanwu Decoction
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Buyang Huanwu decoction (BYHWD) on migration of neural stem cells after middle cerebral artery occlusion (MCAO) in rats. Method: Cerebral ischemia/reperfusion model of MCAO was established in rats using the suture method, successful modelings were divided into ischemia model group and BYHWD group randomly. BYHWD (12 g·kg-1) was administered orally 24 h after ischemia once a day for 21 days. Neurological deficit was assessed by neuroethological assessment. The area of cerebral was determined by 2,3,5-triphenyltetrazolium chloride(TTC) staining. 5-bromo-2-deoxyuridine(BrdU) positive cells were examined by immunohistochemistry, the expression of stromal cell derived factor-1(SDF-1) measured by ELISA. Result: After MCAO the neurological behavioral score, cerebral ischemia area, the number of BrdU-positive cells were significantly increased (P<0.05 or P<0.01). Compared to model group, BYHWD could significantly reduce neurological behavioral score and cerebral ischemia areas(P<0.05 or P<0.01), enhance the SDF-1 protein level (P<0.05). The number of BrdU-positive cells in subventricular zone(SVZ) and corpus striatum also increased markedly(P<0.05 or P<0.01). Conclusion: BYHWD improves migration of neural stem cells in ischemic penumbra after MCAO, the possible mechanism is related to the increased expression of SDF-1.关键词：Buyang Huanwu Decoction;middle cerebral artery occlusion;neural stem cells;migration;stromal cell derived factor-1
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Icariin on the levels of osteopontin(OPN) in rat carotid artery injured by balloon and to evaluate the mechanisms of inhibiting intima hyperplasia. Method: Fourty-two rats were randomly divided into 4 groups: control group,model group and icariin 60 mg·kg-1 group and icariin 120 mg·kg-1 group. Rat carotid balloon injury restenosis model was established. Rats of icariin group were treated with drugs for 2 or 4 weeks. The expression of OPN mRNA were assessed by RT-PCR and immunohistochemistry strategy analysis. Result: Compared with model group,the icariin group significantly decreased the expression of OPN at 14 and 28 day after injury (P<0.01). The difference between two doses was not significant. Conclusion: Icariin could inhibit neointimal proliferation of rat carotid artery injured by balloon. The mechanism might be related to the effects of icariin for decreasing level of OPN, and inhibiting the migration and proliferation of vascular smoothmuscle cells.关键词：icariin;neointima;osteopontin (OPN)
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发布时间：2024-01-04 - 摘要：Objective: To observe gene and protein expression of TRPV5 in bone tissue of rats with dexamethasone induced osteoporosis, and to explain pathogenesis of glucocorticoid-induced osteoporosis(GIO), meawhile to compare the effects of herbs with functions of nourishing-kidney,strengthening-spleen and promoting-blood circulation. Method: One hundrad and thirty two Wistar rats were randomly divided into normal group,model group,nourishing-kidney herb group(1.139 g·kg-1·d-1),strengthening-spleen herb group(0.945 g·kg-1·d-1), activating-blood herb group(0.504 g·kg-1·d-1) and Gushukang group(2.1 g·kg-1·d-1). The rat osteoporosis models was replicate byintramuscular injection of dexamethasone (2.5 mg·kg-1)twice a week, lasted for 9 weeks. The BMD of femur in vitro was detected to determine the bone metabolism marker in serum by biochemical process. The mRNA and protein expression of TRPV5 in bone tissue was detected by real-time quantified PCR and Western blot. Result: The BMD of model group decreased obviously(P<0.01), the content of TRAP in serum increased evidently(P<0.01), mRNA and protein expression of TRPV5 in bone tissue of model group rats increased. In nourishing-kidney herb group, the BMD up-regulated(P<0.01), TRAP down-regulated(P<0.01),and the expression of TRPV5 down-regulated evidently compared with the model group. Conclusion: Nourishing-kidney herbs can inhibit bone resorption of osteoclast through down-regulating mRNA and protein expression of TRPV5 in bone tissue, so have the effect of anti-osteoporosis, which is superior to strengthening-spleen and promoting-blood circulation herbs.关键词：glucocorticoid-induced osteoporosis (GIO);nourishing-kidney;strengthening-spleen;promoting-blood circulation;TRPV5
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发布时间：2024-01-04 -
The Effect of Liguzinediol on Hemodynamics in Rats with Acute Heart Failure Induced by Pentobarbital
摘要：Objective: To investigate the effect of liguzinediol on the hemodynamics in acute heart failure rats. Method: Forth rats were intravenously injected pentobarbital through a micro-infusion pump and randomly divided into 5 groups: saline control group (NS),positive control group (digoxin,22.5 μg·kg-1), three doses of liguzinediol groups (liguzinediol 5,10, 20 mg·kg-1). The heart contractile founctions such as left ventricular systolic pressure (LVSP), maximal rising and falling rate of ventricular pressure (±dp/dtmax), systolic arterial pressure (SAP),diastolic arterial pressure (DAP),heart rate (HR) were measured using a RM6240B/C four channel physiological recording instrument. Result: Liguzinediol induced significant increases of LVSP,+dp/dtmax, -dp/dtmax,and SAP. Conclusion: Liguzinediol induces a positive inotropic effect on the heart in acute heart failure rats.关键词：liguzinediol;acute heart failure;pentobarbital;haemodynamics- 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of curative effect increased of Common Cnidium Fruit-Malaytea Scurfpea Fruit compatibility based on osthole and psoralen as the target components absorbed into the bloodstream. Method: The breast cancer bone metastasis nude mice models were established by injecting MDA-MB-231BO cells into left ventricular, and they were given different combinations of Common Cnidium Fruit-Malaytea Scurfpea Fruit drugs decoction, osthole and psoralen in nude ice plasma were detected by HPLC-MS, and the characteristics of osthole and psoralen absorbed into the bloodstream were observed. Result: The plasma osthole's ion peak could be found in the plasmas of Common Cnidium Fruit group; the psoralen molecule and its ion peak signal could be found in the plasmas of Malaytea Scurfpea Fruit group; osthole molecule and its ion peak signal, but not psoralen molecule or its ion peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit (1: 3) group; not only osthole molecule and its lon peak signal but also psoralen molecule and its lon peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit (2: 2) group; osthole molecule and its ion peak signal, but not psoralen molecule or its ion peak signal could be found in the plasmas of Common Cnidium Fruit-Malaytea Scurfpea Fruit (3: 1) group. Conclusion: Osthole and psoralen could be absorbed into the bloodstream, and the absorption into bloodstream of psoralen could be promoted by Common Cnidium Fruit and Malaytea Scurfpea Fruit 1: 1 compatibility.关键词：Common Cnidium Fruit;Malaytea Scurfpea Fruit;breast cancer bone metastasis model in nude mice
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发布时间：2024-01-04 - 摘要：Objective: To study the fingerprints correlated with pharmacological effect on the basis of LC-MS fingerprint and data of Radix Paeoniae Alba and Radix Paeoniae Rubra, in order to definite the effective compounds of decreasing apoptosis induced by lipopolysaccharide in the alveolar macrophages. Method: The inherent connections were studied between LC-MS fingerprint and decreasing apoptosis of Radix Paeoniae Alba and Radix Paeoniae Rubra, which was induced by lipopolysaccharide in the alveolar macrophages, with partial least squares regression (PLSR). Result: Effective compounds consist of five peaks X8, X10, X3, X7, X13of the fingerprint play the most important role in the decreasing apoptosis induced by lipopolysaccharide in the alveolar macrophages. Conclusion: The 'effective compounds’are the key of quality evaluation of traditional Chinese medicine and can provide comparatively comprehensive information of Chinese medicine.关键词：effective compounds;Radix Paeoniae Rubra;Radix Paeoniae Alba;PLSR;spectrum-effect analysis
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发布时间：2024-01-04 - 摘要：Objective: To establish a test model for phototoxic chemicals with the method of chemiluminescence and assess the phototoxicity of different components of Radix Echinopsis. Method: Nicotinamide adenine dinucleotide reduced(NADH) and photosensitive agents was irradiated with vitraviolet A(UVA), then luminol was added to the test samples, and chemiluminescent value was counted and calculated by an ultra weak chemiluminescence analyzer. Result: Chemiluminescent values of the α-terthienyl concerned with the increase of UVA dosage, and there was a dose-effect relationship in the concentration range of 1.0×10-9-2.5×10-2 g·L-1. Conclusion: This method is simple, accurate and sensitive. It can be used for pre-assessment of phototoxicity.关键词：phototoxicity;chemiluminescence;α-terthienyl;Radix Echinopsis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Bushen Shugan (BSSG) formula on immune function and endocrine system of Lewis lung cancer mice and discuss the possible mechanism. Method: The mice transplanted Lewis lung cancer were randomLy divided into model control group, BSSG group, BSSG combined with cisplatin(DDP) group and DDP group. After two weeks’treatment, the mice were sacrificed and blood was taken from eyeballs, and the tissue of tumor was taken and weighted. The concentration of serum interleukin-2(IL-2), IL-10, estradiol(E2) and testosterone(T) were measured by enzyme-linked immuno sorbent assay(ELISA). Result: Compared with the control group, the BSSG group can inhibit mouse tumor growth and lung cancer metastasis. The content in blood serum of IL-2, T, E2 of BSSG group, DDP group and BSSG combined with DDP group is higher than that of model control group respectively. The level of IL-10 in blood serum of them is lower than model control group respectively. Conclusion: BSSG formula can suppress tumor growth and decrease the number of lung metastatic tumors in the mice; BSSG formula can make an effect on the level of IL-2, IL-10, E2 and T. It can improve the functions of immunity and regulate internal secretion.关键词：Bushen Shugan formula;Lewis lung cancer;interleukin-2(IL-2);IL-10;estradiol(E2);testosterone(T)
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Xiaobanxia Fuling decoction and each decomposed group on the isolated gastric and duodenal smooth muscle movement of rabbit. Method: The experiment uses the organs out of body, and the biologic function analyzer to record the effect of Xiaobanxia Fuling decoction and the aqueous extract of each decomposed group on the rabbits’normal isolated gastric and duodenal smooth muscle and the contraction of isolated gastric and duodenal smooth muscle induced by Ach and atropine. The plot are collected, and the amplitude of vibration, frequency and intension of the isolated gastric and duodenal smooth muscle contraction before and after medication are compared. Result: The effect of Xiaobanxia Fuling decoction and the aqueous extract of each decomposed group on the the isolated gastric and duodenal smooth muscle movement can be recapitulated as the stomach contracts and the duodenum diastoles. It not only has effect on the normal isolated stomach and duodenum, but also has significant antagonistic effect on the rigidity and spasm of gastric and duodenal smooth muscle caused by acecoline(P<0.01); it has synergistic effect on the attenuation of the contraction of duodenal smooth muscle caused by atropine(P<0.05); and it has the effect of excitation on the smooth muscle of the fundus gastricus and the gastric antrum after the use of atropine(P<0.01). The drug potency of Xiaobanxia Fuling decoction and each decomposed group can be listed as: Xiaobanxia Fuling decoction>Xiaobanxia decoction>Rhizoma Zingiberis Recens>Rhizoma Pinelliae. Conclusion: Xiaobanxia Fuling decoction and the aqueous extract of each decomposed group has dual- effect on the isolated gastric and duodenal smooth muscle movement of rabbit; Xiaobanxia Fuling decoction is better than the decomposed group. The way that it plays the role has relationship not only with M choline receptor, but also with other regulatory factors.关键词：Xiaobanxia Fuling decoction and each decomposed group;aqueous extract;isolated gastric and duodenal smooth muscle
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of ilexgenin A on vascular endothelial growth factor(VEGF) and microvessel density(MVD) of tumor in Walker-256 rat model to explore the mechansim. Method: Thirty two Walker-256 rats were randomly divided into 4 groups, and treated with the high dose of ilexgenin A (HD group), low dose of ilexgenin A, 5-Fu (positive group), and normal saline (NS group),respectively. After 8 days of modeling, corresponding drugs were administered by intraperitoneal injection for 14 days. Then, blood rats werr executed to collect blood and remove tumors which were weighted, the morphological observation was performed, aspartate transaminase(AST) and alanine transaminase(ALT) levels were assayed, and the expression of VEGF and MVD in tumor was tested by immunohistochemical method. Result: In treatment group, the weight of tumor obviously decreased than that in model. In treatment group and 5-Fu, the vascular article number intensive degree, compared with model group, was obviously reduced, and ilexgenin A inhibited the elevated level of AST and ALT, the inhibition on AST level was more obvious (P<0.01), the expression of VEGF and MVD levels was significantly decreased compared with model group (P<0.01). Conclusion: Ilexgenin A can significantly inhibit growth of tumor, the mechanism may be related to down-regulating the expression of VEGF and MVD.关键词：ilexgenin A;vascular endothelial growth factor;microvessel density;mechanism of action
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发布时间：2024-01-04 - 摘要：Objective: To separate and screen anti-inflammatory fraction of Isatidis Radix. Method: Membrane separation method was used to separate Isatidis Radix into different sections, their anti-inflammatory effects were evaluated by acetic acid-induced celiac capillary permeability and xylene-induced mouse ear swelling models in mice. Macroporous resin and ion exchange resin were used to further separate Isatidis Radix into different sections, and the anti inflammatory effects were evaluated by abouve mentioned models, finally, their chemical property was determined by color reactions. Result: The substance less than 1 000 in Isatidis Radix had a good inhibiting effect in mice era swelling model, the inhibition rate of swelling being 40.29%(P<0.05). The fraction absorbed by 732 cationic resin in the less than 1 000 substances could inhibit celiac capillary permeability andear swelling, the inhibiting rate being 17.76% and 28.20%, respectively (P<0.05). Color reactions showed that the fraction was composed of amino acids and alkaloids. Conclusion: The amino acids and alkaloids of Isatidis Radix could be the anti-inflammatory fraction.关键词：Isatidis Radix;anti-inflammatory;Active fraction;membrane separation
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发布时间：2024-01-04 - 摘要：Objective: To study the in vitro activity of five furanocoumarins from the root bark of Changium smyrnioides Wolff. isoimperatorin, imperatorin, xanthotoxol, phellopterin and 5-hydroxyl-8-methoxy-psoralen. Method: The antitumor effect against human liver cancer (SMMC-7721 and HepG2), human lung cancer (A549), human gastric cancer (MKN-45), human uterine cervix cancer (Hela) and human breast cancer (MCF-7 and MDA-MB-231) was observed by MTT. Result: Five furanocoumarins had different inhibition on seven kinds of tumors. IC50 was from 0.30 mg·L-1 to 17.23 mg·L-1, especially isoimperatorin was most significantly effective. IC50 was from 0.39 mg·L-1 to 4.11 mg·L-1. Conclusion: These five furanocoumarins have obvious antitumor effect.关键词：root bark of Changium smyrnioides;furanocoumarin;tumor;inhibition
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects and mechanism of Shuwei decotion on the gastric emptying, substance P (SP) and gastric antrum of interstitial cells of Cajal (ICC) in functional dyspepsia (FD) with stagnation of liver-Qi and deficiency of the spleen. Method: The sixty SD rats were randomly divided into 6 groups: Shuwei decoction low dose group, Shuwei decoction high dose group, Muxiang Shunqi pill group, mosapride group,control group and model group, with 10 rats in each group. Shuwei decoction groups were administrated with Shuwei decoction at doses of 7.67 g·kg-1,30.68 g·kg-1, and muxiangshunqi pill group was given Muxiang Shunqi pill of 1.65 g·kg-1, and mosapride group was given mosapride of 1.37 mg·kg-1. The rats FD model ws maded by clasping their tails except the control group.After 3 days,the control and model groups were given distilled water ig(10 mL·kg-1); the normal group and model group were given physiological saline intragastrically; the treated rats were given corresponding drugs for 14 days. To observe gastric emptying, the content of SP were determined by radiommunoassay, structure change of interstitial cells of cajal in gastric antrum was observed by transmission electron microscope. Result: The gastric emptying was delayed and the content of SP obviously decreased(P<0.05) in model group. compared with model group, Muxiang Shunqi pill group (58.35±8.71)%, mosapride group(78.17±10.74)%, Shuwei decoction low dose group(60.39±3.48)% and Shuwei decoction high dose group(76.80±6.96)% improved gastric emptying(P<0.05); compared with SP level(5.32±2.12)ng·L-1 of model group, Muxiang Shunqi pill group(12.89±2.10) ng·L-1, mosapride group(17.86±3.56) ng·L-1,Shuwei decoction low dose group(8.03±2.51) ng·L-1 and Shuwei decoction high dose group(13.79±2.58)ng·L-1 was significantly increased the plasma SP level (P<0.05). The structure change of ICC in gastric antrum improved after treatment. Conclusion: The gastrointestinal motility in FD rats was inhibited, Shuwei decoction can regulate the level of SP, improve the structure and function of ICC in gastric antrum, promote the gastric emptying and recovery the function of gastrointestinal motility.关键词：gastrointestinal motility;functional dyspepsia;Shuwei decoction;interstitial cells of Cajal(ICC);substance P
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Sanhua Tang on expression of MMP-9 in brain tissues of cerebral ischemia-reperfusion rat. Method: The rats were randomly allotted 5 groupa as following: sham operation group, model group, low dose group of Sanhua Tang(7.2 g·kg-1),large dose group of Sanhua Tang(14.4 g·kg-1)ig, and nimodipine group(8.1 mg·kg-1)ig. After 3 days of routine breeding, Sanhua Tang was administered to rats by intragastric administration once every day for a week.The cerebral ischemia reperfusion model was reproduced by blocking middle cerebral arteryt with suture method. The expression of MMP-9 protein was detected by immunohistochemical methods with marketable kit, and mRNA expression of MMP-9 was assayed by RT-PCR. Result: In model group the expression of MMP-9 and MMP-9 mRNA in brain tissues was significantly increased(P<0.01).In large dose group of Sanhua Tang the expression of MMP-9 and MMP-9 mRNA was significantly decreased (P<0.01).In nimodipine group the expression of MMP-9 and MMP-9 mRNA was significantly decreased (P<0.05).In low dose group of Sanhua Tang the expression of MMP-9, and MMP-9 mRNA was less significantly decreased. In large dose group of Sanhua Tang the expression of MMP-9,MMP-9 mRNA was decreased more significantly than in nimodipine group. Conclusion: The mechanism of Sanhua Tang protecting against injure of cerebral ischemia reperfusion may be involved in ameliorating MMP expression.关键词：Sanhua Tang;cerebral ischemia-reperfusion;MMP-9;MMP-9 mRNA
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发布时间：2024-01-04 - 摘要：Objective: To compare the anticonvulsion effect of supercritical-CO2 fluid extractions (SFE-CO2) of Bupleuri Radix, and explore their mechanism. Method: One thousand and sixty mice were randomly divided into 11 groups: RBⅠgroup, RBⅡgroup, RATⅠgroup, RATⅡgroup, ARⅡgroup, UNⅡgroup, PRⅡgroup, SCLⅠgroup, PSⅠgroup, topamax group and NS group. Every group was divided into 4-6 dose groups. The maximal electroshock seizure (MES) was used to observe the anticonvulsion effect. ED50 dose against MES was considered as the administrated dosage to observe the anticonvulsant affects in metrazol seizure test(MET).The rat seizure model was reduced by cortex localion injection of penicillin to investigate the effect of extractions on the convulsant behaviors and hippocampus EEG (the doses were converted by ED95 of MES). Result: ①Except for RBⅡand PSⅠ, all the other seven extractions(PRⅡ, UNⅡ, RATⅠ, PRⅡ, SCLⅠ, RBⅠ,RATⅡ) could dose-dependently antagonize MES in mice. ②All of the seven extractions could prolong the latent period of MET in mice, and the sequence from maximum to minimum was RPⅡ, UNⅡ, RATⅠ, PRⅡ, SCLⅠ, RBⅠ, RATⅡ. The effect of RPⅡ(11.06±4.32)min was better than topamax (9.57±4.47)min.③ The extractions with SFE-CO2 showed a faster onset and shorter duration of he anticonvulsant action than that of supercritical-CO2 alcohol fluid extractions (SAFE-CO2). ④PRⅡ, ARⅡ, UNⅡ, RATⅠ, RATⅡ and RBⅠ could prolong the latent period of epileptiform discharge, reduce the frequency of epileptiform discharge, and decrease the highest wave of hippocampus EEG. Conclusion: ARⅡ, UNⅡ, RATⅠ, PTⅡ, SCLⅠ, RBⅠ, RATⅡ could dose-dependently antagonize MES and MET in mice; PRⅡ, ARⅡ, UNⅡ, RATⅠ and RBⅠ could antagonize penicillin-kindling seizure in rats. PTⅡ may be the most effective extraction in antagonizing the penicillin-induced seizure, and the effect of which may be better than topamax.关键词：extractions with supercritical-CO2 fluid extractions;maximal electroshocfe seizure;metrazol seizure test;penicillin- convulsant model
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发布时间：2024-01-04 -
Effect of Oxymatrine on Expression of c-myc, PSMD9 and CDK4 mRNA in Human Colon Carcinoma LoVo Cells
摘要：Objective: To explore the molecular mechanism of inhibiting colon cancer cell strein LoVo proliferation and inducing apoptosis by oxymatrine (OM) Method: Flow cytometry was used to detect the LoVo cells apoptosis and cell cycle distribution. Fluorescence quantitative PCR was used to detect cell proliferation-related genes like the c-myc, proteasome modulator 9(PSMD9), CDK4 gene expression when LoVo was treated with 0.25,0.5 g·L-1OM. Result: OM had no significant effect on apoptosis in colon cancer LoVo cells when the treatment of OM lasted 48 h and the concentration was lower than 0.5, 0.25 g·L-1 OM can inhibit c-myc gene expression in LoVo when duration of action last 24 h (P<0.05). When the dose increated to 0.5 g·L-1 and duration of action was 48 h, OM could inhibit c-myc, CDK4 gene expression in LoVo cells (P<0.01, P<0.01). When duration of action was extended to 96 h, 0.5 g·L-1 OM could inhibit the c-myc, PSMD9, CDK4 gene expression in LoVo cells(P<0.05,P<0.01, P<0.01). Conclusion: OM at Lower dose could significantly inhibit the proliferation of human colon cancer LoVo cells, the mechanism may be related to reducing c-myc, PSMD9, CDK4 expression in LoVo cells.关键词：oxymatrine;LoVo cells;inhibiting proliferation;apoptosis;c-myc;PSMD9;CDK4- 2
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发布时间：2024-01-04 - 摘要：Objective: To study the antidepressant effect of Jiaotai pill with different proportions. Method: Male Kunming mice were randomly divided into control group, fluoxetine (7.5 mg·kg-1) group and five different proportions of Jiaotai pill in high and low dose groups (Rhizoma Coptidis Granules: Cinnamon Granules 10: 1, 1: 10, 10: 0, 0: 10, 1: 1) according to body weight, togather 12 groups. The tail suspension test (TST) and the forced swimming test (FST) were used to observe the antidepressant effect of Jiaotai pillswith different proportions at 1 h after the administration (ig). Result: Compared with the control group, five different proportions of Jiaotai pill and fluoxetine (7.5 mg·kg-1) reduced the immobility time both in the TST and FST, more obviously in Jiaotai pill (Rhizoma Coptidis Granules: Cinnamon Granules 10: 1 and 1: 10). In the TST, the immobility time of Jiaotai pill (Rhizoma Coptidis Granules: Cinnamon Granules 10: 1) in high and low dose groups was (69.8 ± 18.1) s and (79.2± 14.6) s, respectively; the immobility time of Jiaotai pill (Rhizoma Coptidis Granules: Cinnamon Granules 1: 10) in high and low dose groups was (69.5 ± 21.7) s and (64.1 ± 26.0) s, respectively. In the FST, the immobility time of Jiaotai pill (Rhizoma Coptidis Granules: Cinnamon Granules 10: 1) in high and low dose groups was (83.6 ± 29.0) s and (72.9± 37.7) s, respectively; the immobility time of Jiaotai pill (Rhizoma Coptidis Granules: Cinnamon Granules 1: 10) in high and low dose groups was (92.2 ± 37.8) s and (71.9± 35.9) s, respectively. Compared with the control group, Jiaotai pill (Rhizoma Coptidis Granules: Cinnamon Granules 10: 1 and 1: 10) show an obvious difference in TST and FST test(P<0.05 or P<0.01). The same results in the TST werefound in the FST (P <0.01). Conclusion: The results suggested that different proportions of Jiaotai pill performed antidepressant effect, while there may be a difference in intensity of the effect.关键词：Jiaotai pill;depression;tail suspension test;forced swimming test
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发布时间：2024-01-04 -
Effect of Cistanches Herba on the Swimming Ability and Oxidation Resistance of Mitochondrial in Rats
摘要：Objective: To study the effectd of Cistanche Herba on swimming ablity antioxidant resistance of. mitochondrial in rats Method: One hundred and twenty male Wistar rats were randomly divided into static ig water group (C group), still ig Cistancht Herba group(M group), exercise ig water group (T group), group exercise ig Cistanches Herba 1-4 (TM 1-4 group). A professional device ig 1 times a day. TM 1-4 ig dose group were 6.01,9.97,13.94,17.90 g·kg-1, ig volume 5 mL·kg-1, C, T two groups ig distilled water. 4 weeks after exhaustive swimming training, measured body weight, swimming time and heart mitochondria in malondialchehy-che(MDA), superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) levels and other indicators related with fatigue. Result: TM weight of each group is greater than the T group (P<0.05), swimming time longer than T group (P<0.01), TM each group no significant differences. Result of exhaustive swimming in the MDA content in rat heart mitochondria was significantly increased,T-group and TM 1-4 group were increased to (5.96±0.71), (5.05±0.36), (5.05±0.92), (5.06±0.16), (5.05 ±0.25) μmol·L-1, and that the TM groups were significantly different lower than the T group (P<0.01); SOD, GSH-Px activity was significantly decreased (T-group and T1-T4 group, SOD decreased to (209.78±17.21),(249.85±10.93),(253.98±9.64),(259.31±14.57),(259.46±15.73) U·mL-1, GSH-Px decreased to (57.95±11.95),(68.58±6.01),(70.63±5.83),(70.78±7.04),(72.56±4.34) U·mg-1, and that the TM groups were significantly higher than the T group (P<0.01); TM of each group showed no significant difference. Conclusion: Cistanches Herba has improved exercise capacity in rat heart mitochondria and the role of antioxidant enzymes, there by reducing free radicals on myocardial sarcoplasmic reticulum and mitochondrial membrane damage, inhibition of high-intensity exhaustive exercise cause myocardial mitochondrial oxidative damage, delay fatigue occurred; have a significant effect at a dose of 6.01 g·kg-1.关键词：Herba Cistanches;exercise capacity;anti-free radical;myocardium;mitochondria- 2
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Shexiang Wulong Wan(SXWLW) on the pathomorphology and tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in synovium rats with adjuvant arthritis. Method: Adjuvant arthritis (AA)animal model was reproduced.Forty Wistar male rats were divided randomly into 4 groups.model group, normal group treated with normal saline (NS)of 2 mL every day; SXWLW low dose group treated with 1.0 g·kg-1 every day; SXWLW high dose group treated with 2.0 g·kg-1 every day. At the end of the experiment, toe metatarsal joints were collected for pathological examination.The level of TNF-α and IL-6 was checked by immunohistochemical methed. Result: SXWLW low dose and high dose groups,compared with the model group, synovial tissue of damage was significantly reduced; the expression of TNF-α and IL-6 in the SXWLW low dose group and high dose group, compared with model group,decreased (P<0.01 or P<0.05). Conclusion: SXWLW can reduce the expression of TNF-α and IL-6 in the synovial tissue,therefore it could decrease synovial tissue damage.关键词：Shexiang Wulong wan;adjuvant arthritis;rheumatoid arthritis;tumor necrosis factor-α;interleukin-6
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Yangxinkang tablet on cardiac function,tumor necrosis factor-α(TNF-α) and interleunin-6(IL-6) in rabbit with chronic heart failure. Method: The rabbit heart failure animal model was established by blocking left anterior descending coronary artery. The rabbits were randomly divided into model group,captopril group, low dose group, middle dose group and high dose group of Yangxinkang tablet,the normal control group was set, with 5 rabbits in each group. After 5days of modelling, captoprilat dose of 1.2 mg·kg-1and Yangxinkang tablet at dosage of 0.17,0.51,1.53 g·kg-1were orally administrated once a day for 4 weeks. The effects of Yangxinkang tablet on cardiac function, TNF-α and IL-6 were investgated. Result: The left ventricular end diastolic volum(LVEDV) in control group was lowest(2.20±1.14)mL, compared with model group (4.12±1.19)mL, the difference was statistically significant(P<0.01), and compared with model group, middle dose group, high dose group and captopril group showed satistically significant (P<0.05). LVEF was 64.80%±12.07% in model group, middle dose group and high dose group indicated statistically significant(P<0.01),compared with model group.The level of TNF-α and IL-6 in the model group was highest, the differences in Yangxinkang tablet groups and captopril were statistically significant compared with model group (P<0.01). Conclusion: Yangxinkang tablet can improve cardiac function, reduce serum TNF-α and IL-6 levels in rabbits with heart failure.关键词：Yangxinkang tablet;chronic heart failure;cardiac function;tumor necrosis factor-α;inter leukin-6
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Epimedium pubescens Maxim.(EPM)on osteoporosis in ovariectomized mouse. Method: Kunming mice were used in this study.Osteoposis model was estabished by ectomizing the ovarians of the mice. The mice were divided into six groups randomly: control group, model group, diethylstilbestrol positive control group, high dose of EPM group(200 mg·kg-1), middle dose of EPM group(100 mg·kg-1) and low dose of EPM group(50 mg·kg-1).Sixty days after administration, bone density, the osteoclast and intelukin (IL-1β) of the bone tissue were measured. Result: The model group animals had obvious osteoporosis. EMP increased bone density, and decreased the osteoclast number and IL-1β obviously. Conlusion: EPM can increase the bone density. EPM has an apparent role of anti-osteoporosis in ovariectomized mice. And it’s mechanism may be related to the reducing of the level of IL-1β.关键词：Epimedium pubescens Maxim.;osteoporosis;intelukin-1β;bone density
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of Danggui Shaoyao San(DSS) on experimental myocardial ischemia in mice. Method: One hundred healthy male Kunming mice were divided into five groups at random:blank control group, model group, DSS low-dose group (raw herb 30 g·kg-1·d-1), DSS high-dose group (60 g·kg-1·d-1) and positive control group (Fufang Danshen Diwan 0.174 g·kg-1·d-1). Each group was fed with according medicine from the first day to the fifth day. On the fifth day injections of Pit (30 U·kg-1) was given to every group intraperitoneally except the blank control group to which injections of saline were given intraperitoneally. The myocardial ischemia model was duplicated by pit. The electrocardiogram at the tenth minute before the Pit injection and the 5, 15, 25 min after the Pit injection was recorded. Thirty minutes after the Pit injections the mice were sacrificed, the blood was obtained and the contents of NO were checked up. The tip of myocardium was made into pathological specimen to observe the activities of eNOS by immunohistochemitry method. The rest myocardium was made into tissue homogenate with saline for checking up the activity of SOD and the content of MDA. Result: Compared with the model group, the heart rates in the DSS high-dose and the low-dose groups were recovered significantly (P<0.01 or P<0.05). The contents of serum NO in the DSS high-dose group, the low-dose group were increased significantly (P<0.01). The activities of eNOS in the DSS high-dose group and the low-dose group were enhanced. The activities of SOD in the DSS high-dose group and the low-dose group were increased significantly (P<0.01), while the contents of MDA were decreased significantly (P<0.05 or P<0.01). Conclusion: The classical prescription DSS could prevent and treat myocardial ischemia injure effectively, protect the myocardial cell.关键词：Danggui Shaoyao San;myocardial ischemia;pituitary;electrocardiogram;free radical;nitric oxide
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effec of combined application of Herba Asari and Radix Clematidis in relief of pains. Method: Two hundreds and twenty-nine cases involved three kinds of pain syndroms, patients with different pain syndroms were divided into four groups,respectively: the Herba Asari group was treated with Herba Asari; the Radix Clematidis group was treated with Radix Clematidis; the combined application group was treated with Herba Asari and Radix Clematidis.Treatment lasted 2 weeks.The effecicy of relieving pain was observed. Result: Each group showes curative effect of pain relief(P=0.000), and efficacy in group of combined appication was more significant. Conclusion: The combined appication of Herba Asari and Radix Clematidis has a good analgesic actions关键词：Herba Asari;Radix Clematidis;pain;clinical observation
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发布时间：2024-01-04 - 摘要：Objective: To observe effect of of Shenfu Shexin decoction on Brain Natriuretic Peptide(BNP) in elderly patients with coronary heart disease and heart failure. Method: Sixty-five elderly patients with coronary heart disease and heart failure were divided into two groups. The control group and the observation group. Thirty-two cases in control group were treated by routine treatment, thirty-three cases in observation group were treated by combined routine treatment and Shenfu Shuxin decoction. The effect of BNP were observed in two groups. Result: The curative efficacy was obviously higher in the observation group than in the control group. The expression of BNP was obviously decreased after treatment. But the decrease value of BNP was higher in the observation group than in the control group. Conclusion: The treatment of Senfu Shuxin decoction can improve the curative effect, and decrease the expression of BNP in patients,therefore it is helpful to elderly patients with coronary heart disease and heart failure.关键词：coronary heart disease;heart failure;Senfu Shuxin decoction;BNP;clinical observation
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the clinical therapeutic effect of respiratory system emergency by Chuankezhi injection. Method: Eighty-six patients with acute respiratory system, were randomly divided into treatment group and control group, 44 cases in treatment group, 42 cases in the control group. Control group was received conventional oxygen, antiinflammatory, symptomatic of treatment. The treatment group patients were intramuscularly injected Chuankezhi injections 4 mL·d-1, 7 days being a course of treatment. The symptoms and signs, such as asthma, cough, and supporting test results were observed before and after treatment. Result: Total effective rate was 88.64% in the treatment group and 66.67% in the control group, and obvious difference appeared between the two groups (P<0.05). Conclusion: The effective was of Chuankezhi injection in respiratory system emergency is significant.关键词：Chuankezhi injection;respiratory system emergency;clinical observation
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发布时间：2024-01-04 - 摘要：Objective: To observe the preventive effect of Shenghua Guben decoction on nausea and vomiting caused by chemotherapy scheme involved cisplain. Method: A randomized crossover controlled design was used,86 patients were randomly allocated to two groups, the Shenghua Guben decoction treatment group and the tropisetron control group. Result: The control rate of nausea was 82.6% and 74.4%in control group and treatment group, respectively. The control rate of vomiting was 87.2% and 88.4%in control group and treatment group,there was no significant difference between two groups. The rate of adverse reactions was 32.56% in control group, compared with 1.16% in treatment group, there was a significant difference between two groups(P<0.05). Conclusion: The Shenghua Guben decoction could effectively prevent and treat nausea and vomiting induced by chemotherapy scheme including cisplain, and the adverse reactions were decreased.关键词：Shenghua Guben decoction;cancer;cisplain;chemotherapy;nausea;vomiting
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发布时间：2024-01-04 - 摘要：Objective: To observe syndrome patterns of chinese medicine and risk factors in metabolic syndrome(MS) complicated impaired glucose regulation(IGR) with combination of phlegm and heat and deficiency of both Qi and Yin. Method: The clinical study was conducted in 353 patients with MS complacated IGR from Beijing, Shandong and Liaoning province. The following indexes were observed, including body mass index(BMI), waist cincumference(WC), systolic blood pressure(SBP), diastolic blood pressure(DBP), total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL), low density lipoprotein(LDL), fasting plasma glucose(FPG), postprandial blood glucose(PBG), glycolated hemoglobin(HbAlc) Result: ①BMI and WC in combination of phlegm-heat group were higher thandeficiency Qi and Yin group(P<0.05).②PBG in combination of phlegm-heat group group was higher than deficiency of Qi and Yin group(P<0.05).③No correlation was found in chinese medicine syndrome patterns. ④LDL in combination of phlegm-heat group was higher than deficiency of Qi and Yin group(P<0.05). Conclusion: BMI, WC, PBG and LDL are risk factors in combination of phlegm-heat group, attention should be payed to treatment of combination phlegm-heat syndroms in clinical.关键词：impaired glucose regulation(IGR);metabolic syndrome(MS);Chinese medicine syndrome patterns;clinical study.
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the effect of 'Inducing and Expelling’principle in traditional Chinese medicine for the treatment of psoriasis. Method: One hundred cases of psoriasis treated by Yinxiaoping granule(4.5, 9.0 g·d-1)were included in the retrospective analysis. The treatment duration depended on the conditions of the patients. Statistical analysis was carried out for the analysis based on patient’s condition, the degree of the suffering and the recurrence. Result: The analysis indicates that the longer the suffering duration was, more serious the patient’s condition, and more frequent the recurrence (P<0.05 or P<0.01). In the case of a long treatment of western medicine has been taken, the treatment of Yinxiaoping granule was extended accordingly. The inducing duration, the extinctive duration and the recovery duration were also extended respectively(P<0.05 or P<0.01). There was no need to be dietetic restraint for the treatment of 'Inducing and Expelling’. Some of the inducing food even could be helpful for the efficacy. Yinxiaoping granule decreased and delayed the recurrence. Conclusion: Yinxiaoping granule decreases and delays the recurrence of psoriasis. The treatment of 'Inducing and Expelling’opens a new concept and approach for the treatment of psoriasis.关键词：psoriasis;treatment;inducing and expelling;recurrence
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发布时间：2024-01-04 - 摘要：Objective: Herbal formula, as the basic therapeutic form of traditional Chinese medicine (TCM) practice, is used under the guidance of syndrome (also called pattern or Zheng) differentiation based on TCM holistic view. Understanding the principle of herbal formulae is not only one of great dilemmas in current studies but also one of highlight on TCM. Based on previous omics methods and in silicon techniques, network pharmacology was used to explore TCM pharmacology, compounds combination, herbal property and efficacy, and so on. In present review, we outlined some proper strategies to construct TCM cold or hot syndrome related rheumatoid arthritis disease network, to set herbal formulae related biological targets network, to verify the TCM syndrome based rheumatoid arthritis disease network, and further to clarify the basis of material and the principle of herbal formulae. Hopefully, the novel paradigm and applications in the review could provide reference for the coming TCM network pharmacology.关键词：rheumatoid arthritis;formulae of Chinese medicine;network pharmacology
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发布时间：2024-01-04 - 摘要：The animal models of chronic obstructive pulmonary disease were reviewed and analyzed. It is hoped to provide a new train of chronic obstructive pulmonary disease (COPD) therapeutic drug development. Data on COPD and animal models in recent years were searched by the CAS Shanghai institute of materia database platform. The advantages and disadvantages of several animals as a COPD animal model were summarized, and the methods for COPD model and the relevant indicators to judge the successfulness of COPD model were established. Because of the complex pathogenesis of the COPD, it was failed to approach a unified COPD modeling standard. The article reported that several method unions are mostly used. This review hopes to provide some reference for the new treatments for COPD drug development. We expect to establish a standardized model of COPD for the disease treatment and research in the future关键词：chronic obstructive pulmonary disease;animal models;pharmacological
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发布时间：2024-01-04 - 摘要：To analyze and review on the literature regarding miRNA research. 21 literature was retrieved and selected from MEDLINE and CNKI database using miRNA as subject heading,then after the new progress on miRNA research and application in traditional Chinese medicine was reviewed. miRNA was a new kind of biomarker in diagnosis and therapeutic effect evaluation, and an emerging target in treatment and drug intervention, meanwhile a new active component of Chinese herb. Broad prospects of miRNA application will be of significance for promotion of traditional Chinese medicine research.关键词：microRNA;disease;target;active component;traditional Chinese medicine research
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发布时间：2024-01-04 - 摘要：Summary and reflection the research status of four natures of Chinese herbal medicine,and a novel idea is proposed based on photon emission detecting.Properties of Chinese herbal medicine are the property and nature of drugs which are related to the efficacy, and are the capacity of adjusting the state of body according to the traditional chinese medicine theories. Through analyzing the traditional Chinese medicine concepts and state on the research of the medicinal herbs, and based on the viewpoint that Qi can be characterized by electromagnetic radiation, we think that four properties is the measurement which may be used to regulate the quantum of electromagnetic radiation in human body.At present, Due to the defects in the research methods of four natures,it is will be meaningful exploration to analysis four natures based on photon emission.Detecting photon emission of different Chinese herbal medicines may reflect differences between them, it provides a new idea for revealing the nature of four properties of traditional Chinese medicine.关键词：Chinese medicine four natures;electromagnetic radiation;biophoton
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发布时间：2024-01-04 - 摘要：This paper was to arrange and classific analyze literature of macroporous resin technology in application for traditional Chinese medicine(TCM),Including separation and purification of active ingredients from single or compound prescription of TCM,removal of harmful impurities from TCM,conformity organic residues in resin, regeneration of macroporous resin and so on.And to investigate expansion of application field of macroporous resin technology.Separation technology of macroporous resin was mainly used in enrichment of saponins,flavonoids,alkaloids and so on,but in recent years,it was used in removal of heavy metal impurities and pesticide residues.According to structure type of TCM compound,to develop high selectivity of resin,separation efficiency of active ingredients from TCM was improved,in the future,fast,time-saving,low cost will be new trend.31 articles were selected which published in 2009-2011 years,these articles took up more than 62% of total references,hope for providing reference for research and production of macroporous resin technology.关键词：macroporous adsorption resin;traditional Chinese medicine;extraction and purification
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发布时间：2024-01-04
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