最新刊期
卷 18 ， 期 4 ， 2012
- 摘要：Objective:To optimize preparation technology of Dracocephalum moldevica flavonoids skeleton-type pellets by extrusion spheronization method.Method:D.moldevica flavonoids skeleton-type pellets were prepared by extrusion spheronization method;Optimal formulation and process was obtained by single factor test and orthogonal design;Micromeritic properties and cumulative in vitro release of pellets were determined.Result:D.moldevica flavonoids skeleton-type pellets were prepared by extrusion spheronization method were all good of roundness,fluidity and density,product yield was high,in vitro release reached more than 80% in 30 min.Conclusion:D.moldevica flavonoids skeleton-type pellets were prepared by extrusion spheronization method,this preparation process was simple and feasible,quality of prepared pellets was excellent and product yield was high.关键词：total flavonoids from Dracocephalum moldevica;skeleton-type pellets;extrusion sphemnization method
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发布时间：2024-09-23 - 摘要：Objective:To study on extraction process of volatile oil from dried ginger and its inclusive compound by β-cyclodextrin（β-CD） from Sini effervescent tablet.Method:Orthogonal test was employed to investigate effect of water volume,soak time and distilled time on extraction of volatile oil;and took comparative test of saturated water solution method,colloid mill method,and ultrasound method,choose optimum inclusion method with inclusion efficiency of volatile oil,yield of inclusion and the content of oil in inclusion as comprehensive indexes;Optimum inclusion technology of colloid mill method was optimized by orthogonal test,and validate inclusion compound.Result:Optimum extraction technology conditions were to add 6 times the amount of water,and distilled 8 h without soaking;Optimum inclusion technology of colloid mill method was as follows:proportion of volatile oil to β-CD was 1∶8（mL∶g）,inclusion time was 45 min;the amount of water was 80 mL.Conclusion:Inclusion technology（colloid mill method）of volatile oil from dried ginger was simple,feasible and appropriated for large-scale production.关键词：volatile oil from dried ginger;extraction;colloid mill;β-CD;orthogonal design;multi-index
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发布时间：2024-09-23 - 摘要：Objective:To study on optimum formulation and preparation technology of curcumol liposome by ethanol injection method.Method:curcumol liposome were prepared by ethanol injection method;taking entrapment efficiency and stability parameter as indexes,formulation and preparation technology were optimized by orthogonal test;entrapment efficiency of liposome was determined by HPLC.Result:Optimum formulation was:phospholipids 70 g·L-1,phospholipids-cholesterol 8∶1,pH of phosphate buffer 6.0,concentration of phosphate 0.10 mol·L-1;Optimum extraction technology was:probe ultrasonic time 4 min,magnetic stirring speed 30 r·min-1,water bath temperature 50 ℃,appearance of prepared curcumol liposome was uniform round,with entrapment efficiency was 62.91%,average particle size was 124 nm and average zeta-potential was-26.7 mV.Conclusion:preparation process of ethanol injection was simple and easy to control.optimized formulation and preparation of curcumol lipsome was stable and feasible.关键词：curcumol;liposome;orthogonal test;ethanol injection method
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发布时间：2024-09-23 - 摘要：Objective:To investigate optimum extraction technology of Kanggan Jiedu granule.Method:Optimum extraction technology of volatile oil was screened with yield of volatile oil as index,water extraction technology was optimized by yield of extract and the content of puerarin as indexes.Result:Optimum extraction technique of volatile oil from Japanese Honeysuckle and others was:extracted 5 h with 10 times the amount of water.The best extraction technique of Radix Puerariae and others was:extracted 1,0.75,0.75 h three times with 8,8,6 times the amount of water respectively.Conclusion:Optimum extraction technique was stable and feasible.关键词：Kanggan Jiedu granule;extraction technology;optimal
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发布时间：2024-09-23 - 摘要：Objective:To prepare Hawthorn leaves flavonoids solid self-emulsifying granule（HLF-SSEG） and evaluate its quality.Method:With degree of emulsification and emulsifying time as indexes,self-emulsifying prescription was optimized by solubility experiment and ternary phase diagrams.Screened prescription of HLF-SSEG by comprehensive investigating forming ratio of different prescription proportion,re-dispersion and in vitro dissolution,and its particle size and zeta potential,DSC and X-diffraction scan and dissolution were examined.Result:Composition of optimized optimum formulation was as follows:Hawthorn leaves flavonoids-tween80-transcutol P-Castor oil-starch-lactose-mannitol 1∶1.5∶1.35∶0.15∶12∶2∶2,average particle size was（169.3±7.6） nm after dispersed,dissolution was 89.5% in 30 min.Conclusion:HLF-SSEG was easy to prepare and its quality was stable.The dissolution of HLF was dissolution improved by SSEG.关键词：Hawthorn leaves flavonoids;solid self-emulsifying;dissolution
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of Gushudan by smashing extraction method.Method:Smashing extraction technology of Gushudan was studied by the content of total flavonoids and total coumarins,the total contents of naringin,epimedin B,icariin,osthole and tanshinoneⅡA,and extract ratio from Gushudan as indexes.Result:Optimum extraction technology was as follows:smashing extracted 3 times with 12 times the amount of 75% ethanol as dissolvent,2 minutes every time.The content of total flavonoids,total coumarins,total contents of naringin,epimedin B,icariin,osthole and tanshinoneⅡA,and extract ratio were 26.27,17.93,9.213 mg·g-1,and 23.50% respectively.Conclusion:Optimum smashing extraction technology was stable and easy.关键词：Gushudan;orthogonal design;smashing extraction;reflux extraction
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发布时间：2024-09-23 - 摘要：Objective:To optimize microwave extraction conditions of mixed water-soluble from Codonopsis pilosula and Poria cocos.Method:Uniform design method was usd to investigate effects of microwave power,extraction time,ratio of solid-solvent and extraction times on the content of mixed polysaccharide.Result:Optimum microwave extraction technology conditions were as follows:microwave power was 130 W,extraction time was 6 min,ratio of solid-solvent was 1:40（g·mL-1）,extraction times was 3 times,yield of mixed water-soluble polysaccharide was 15.79% by microwave extraction,and it was 15.11% by conventional extraction method.Conclusion:Microwave extraction and conventional extraction of mixed polysaccharide showed no significant difference,but because of microwave extraction method was rapid,simple,it was suitable for modern production.关键词：Codonopsis pilosula;Poria cocos;mixed polysaccharide;uniform design;microwave;fourier transform infrared spectrometer
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发布时间：2024-09-23 - 摘要：Objective:To optimize self microemulsion formulation of Wolfberry seed oil and to preliminary evaluate its performance in vitro.Method:Kind of surfactant and oil phase were optimized with pseudo ternary phase diagram was prepared by water titration method;Proportion of each component from prescription were determined by comparing transmittance of system,self emulsifization time of formulation and its stability under several conditions（centrifugation,freezing,heating and other conditions） were evaluated.Result:Optimum formulation of Wolfberry seed oil was composed of 10% Wolfberry seed oil,20% ethylise oleas and 70% Tween 80.This formulation took less than 10 minutes to form spontaneously to be opalescent transparent latex with small and uniform particle size,it showed good stability of kinetic,but sensitive to heat.Conclusion:Optimum formulation of Wolfberry seed oil showed good self emulsification performance and acceptable stability.关键词：Wolfberry seed oil;self microemulsifying;pseudo ternary phase diagram;quality assessment
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发布时间：2024-09-23 - 摘要：Objective:To study on forming technology of Guiqi polysaccharide granule.Method:With hygroscopicity,solubility and forming rate as index,kinds and ratio of excipients and optimum forming process of formulation were screened out.Result:Optimum granulating condition:ratio of raw materials-mannitol-PVP was 1∶0.9∶0.1,wetting agent was zein ethanol solution.Conclusion:Selected excipients of granule was reasonable,molding technology was convenient and feasible.关键词：Guiqi polysaccharide;granule;formulation process
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of Aikeqing capsule,a compound preparation of Chinese medicine.Method:Orthogonal test was used to evaluate extraction technology with yield of icariin,polydatin,glycyrrhizic acid and baicalin as indexes,optimum technology conditions were optimized by combined with comprehensive scoring method.Result:Optimum extraction conditions were as follows:H.Epimedii was heating refluxing extracted 2 times with 30 times the amount of 65% ethanol for 3 hours each time,extraction solution was concentrated in vacuum to relative density of 1.10（60 ℃）;then treated with 3 times the amount of 20% ethanol staying for 8 hours to filter off chlorophyll;extracted 3 times of mixed herbs powder of R.polygoni cuspidate,R.scutellaria,and R.glycyrrhizae with 10 times the amount of 80% ethanol for two hours each time.Conclusion:After tested by pilot,yield of maker compounds were 66%～88%,it showed optimized technology was feasible.
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发布时间：2024-09-23 - 摘要：Objective:To prepare tanshinone release pellets and research its preparation technology.Method:Tanshinone was prepared for release solid dispersion,influence of ethyl cellulose（EC） and hydroxypropyl methyl cellulose（HPMC） in different dosage on dissolution was investigated;Tanshinone solid dispersion as principal agent was prepared for pellets,effects of wetting agent,drug loading dose,accessories,lactose,wetting agent dose and other factors from prescription on yield and roundness of tanshinone were investigated;Optimum preparation technology of tanshinone release pellets was screened out by orhogonal design.Result:Tanshinone was prepared for release solid dispersion,prescription was tanshinone-EC-HPMC 1∶3∶0.5;Pellet was prepared by solid dispersion as principal agent,Formulation was tanshinone solid dispersion 20 g,lactose 6 g,MCC 24 g,33 mL water as wetting agent,optimum technology conditions were:extrusion frequency 25 Hz,rounded frequency 50 Hz,spheronization time 6 min.Conclusion:This process was scientific and rational,preparation was stable,it settled basis for preparation of multi-drug delivery system of Tongmai compound pellets.关键词：multi-drug delivery system of Tongmai compound pellets;tanshinone release unit;solid dispersion;extrusion spheronization;orhogonal design
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发布时间：2024-09-23 - 摘要：Objective:To study on preparation technology of dry extract from Pueraria thomsonii.Method:Extraction technology of P.thomsonii was optimized by single factor and orthogonal experiment by taking the content of puerarin as index.Technology steps of dry extract from P.thomsonii was estimated by the content of puerarin and transfer rate of main feature peak area of specific chromatogram as index.Result:Optimum extraction technology was established as follows:heating reflux extracted 3 times with 8 times the amount of 30% ethanol for 2 h each time,extraction ratio of puerarin was more than 97%;Main preparation technology steps were:decoction pieces→alcohol extract fluid→concentrated solution→alcohol precipitating fluid→extract→dry extract,transfer ratio of puerarin in technology steps were 97.89%±2.17%,81.70%±2.12%,74.92%±1.92%,72.15%±1.06%,50.64%±2.57%（n=3）,it showed puerarin losed more in drying step;It showed in specific chromatogram that all technology steps remained 7 characteristic peaks of fingerprint,and transfer ratio of peak 1 and peak 6 was high,but it was low of peak 2,3,4,5,7;Yield of dry extract from P.thomsonii was 5.09%±0.06%,and the content of puerarin was（29.262±1.500） mg·g-1.Conclusion:This technology was simple,feasible and could provide a reference for technology of P.thomsonii compound preparation.关键词：dry extract from Pueraria thomsonii;orthogonal experiment;puerarin;specific chromatogram
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发布时间：2024-09-23 - 摘要：Objective:To optimize preparation process of Qiangxin capsule.Method:Orthogonal experiment and comprehensive scoring method were used to optimize water extraction technology;Forming process and production conditions were studied by multi-factor comparison.Result:Established an HPLC-ELSD method for determination of astragaloside Ⅳ.optimum extraction process was as follows:extracted 3 times with 9 folds the amount of water for 1.5 h each time;Ratio of excipients and optimum wetting agent when making particles of filling material in capsule were determined,relative humidity should be lower than 67%.Conclusion:This preparation process was reasonable and feasible,it could provide theory envidence for industrial production of Qiangxin capsule.关键词：Qiangxin capsule;orthogonal test;astragaloside Ⅳ;composite scoring;preparation process
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发布时间：2024-09-23 - 摘要：Objective:To optimize ultrasonic-assisted extraction technology of flavonoids from peels of Carya cathayensis.Method:Based on peels of C.cathayensis as raw materials,60%ethanol as extraction agent,ultrasonic power 250 W,used quadratic general rotation unitized experimental to design.Based on single factor test,selected ratio of solid-liquid,extraction temperature and extraction time as factors,established regression mathematics model with yield and purity of flavonoids as target.Then got optimization of extraction conditions for flavonoids by analysing variance of test results and optimizing mathematical model.Result:Optimum ultrasonic-assisted extraction technology conditions of flavonoids were as follows:ratio of solid-liquid 1:12,extraction temperature 72 ℃,extraction time 52 min,under these conditions,yield of flavonoids was 2.19%,purity could reach 16.62%.Conclusion:This mathematics model for ultrasonic-assisted extraction of flavonoids from peels of C.cathayensis was feasible.关键词：peels of Carya cathayensis;flavonoids;quadratic general rotation unitized design;ultrasonic-assisted extraction
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发布时间：2024-09-23 - 摘要：Objective:To comparative study on removal method of protein for polysaccharide from Liuwei Dihuang biological preparation,and investigate effect on yield and purity of polysaccharide by different methods.Method:Sevage method,TCA-butanol precipitation method and tannin acid precipitation method were taken to deproteinized of Liuwei Dihuang biological preparation,and compared effect of three deproteinization methods by deproteinization rate and polysaccharides retention rate.Result:Deproteinizationhe effect of TCA-butanol precipitation method was the best,optimum conditions were:volume ratio of TCA-butanol reagent was 1∶2,ratio of TCA-butanol reagents was 1∶5,vibration time was 30 min,standing for 1.0 h,which showed highest efficient of deproteinization for polysaccharides and higher polysaccharides retention rate.Conclusion:TCA-butanol method was optimum technology of removing protein from Liuwei Dihuang biological preparation.关键词：polysaccharide;deproteinization;Sevage method;TCA-butanol method;tannin acid method
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction and purification technology of total flavonoids for S.baicalensis and S.sarmentosum from Longchaifang.Method:The content of total flavonoids were selected as index,extraction and purification technology condition of total flavonoids was optimized by orthogonal test.Total flavonoids was purified by macroporous resin,screened optimum purification macroporous resin by static adsorption and desorption test;Optimum elution condition was examined by related indicators of dynamic adsorption.Result:Optimum extraction process was as follows:reflux extracted 2 times with 15 times the anount of 60% ethanol at 85 ℃,2 h each time.Optimum purification technology was as follows:AB-8 macroporous resin was adopted,concentration of sample was 0.11 g·mL-1,adsorptive speed was 3 BV·h-1,adsorbing column was eluted with 5 BV waterand 8 BV 70% ethanol.Conclusion:The content of the total flavonoids was high by optimization technology.This technique was simple and suitable for modern production.关键词：Longchaifang;total flavonoids;orthogonal design;macroporous resin
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发布时间：2024-09-23 - 摘要：Objective:To optimize preparation process of berberine-β-cyclodextrin inclusion complex by central composite design and response surface method.Method:With inclusion ratio and yield of berberine-β-cyclodextrin inclusion complex as indexes,influence of inclusion temperature,inclusion time,and ratio of β-CD-berberine to inclusion process was investigated by response surface method,results were fitted by multi-linear equation,second-order and third-order polynomial equation,and optimal inclusion process was predicted according to formulation.Result:Multiple correlation coefficients from multi-linear equation were worse,and multiple correlation coefficients from third-order polynomial equation were prior to second order polynomial equation.Optimum inclusion technology of berberine-β-cyclodextrin inclusion complex were as follows:ratio of β-CD-berberine 3.65∶1,inclusion time 4.5 h,inclusion temperature 64 ℃.Bias of inclusion rate and yield between observed and predicted values were 3.28% and 2.63% respectively.Conclusion:Using central composite design and response surface methodology to optimize preparation of berberine-β-cyclodextrin inclusion complex had good prediction.关键词：berberine;cyclodextrin;central composite design;response surface methodology;inclusion process
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发布时间：2024-09-23 - 摘要：Objective:To choose the optimization conditions for the formation of total flavonoids in herba epimediumon complex with phospholipid.Method:Solvent evaporation process was used to prepare total flavonoids in herba epimediumon phospholipid complex.The evaluation criterion is complex ratio.The experiment inspect the significance of influential factor of preparation technology by Plackett-Burman design.Optimized significance of factor and level with the Box-Behnken design.The binomial and linear equations were fitted to the data of overall desirabilities,and the resulting equation was used to produce 3-D response surface graphs,through which optimal formulation were predicted.Result:Total flavonoids and phospholipid ratio,flavonoids concentration and reaction temperature had significance influence to the craft.It showed that the correlation coefficient of second-order quadratic model was high.Correlation coeficient reached 0.988.The optimum conditions for the preparation of total flavonoids in epimediumon complex were as follows:phospholipids-total flavonoids being 3.6∶1,time 2.4 h,temperature at 40 ℃ and concentration of principal agent being 10 g·L-1.The deviation between result of the best craft verification test and the binomial fitting equation forecast value is less than 2.0%.Conclusion:Response surface methodology was successfully used to optimize the preparation of total flavonoids in herba epimediumon phospholipid complex.Optimized process was reliable,stable and available for industrial production.关键词：total flavonoids in herba epimediumon;phospholipid complex;Plackett-Burman design;Box-Behnken design;response surface method
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发布时间：2024-09-23 - 摘要：Objective:To optimize the process of preparing dehydrodiisoeugenol chemical reference substance.Method:Taking extract rate of dehydrodiisoeugenol as index,the best extraction condition of dehydrodiisoeug-enol was determined by single factor test and orthogonal test L9（34）,which was scaled up to pilot volume.Result:Raw material was extracted with 80% ethanol at a 10∶1 solid-liquid ratio for twice and 2 h each time,the yielded extract was first seperated by extraction with absolute ethyl alcohol、acetyl acetate.Afer this,by the methods of silicagel column chromatography and recrystallization we produce 9 g of reference substance was producued.Conclusion:The process is effective,simple and economic,and can meet the need of gram-size preparation.关键词：dehydrodiisoeugenol;reference substance;Myristica fragrans
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》由国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的学术刊物,已成为"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊";"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术期刊光盘版"全文收录期刊;并被评为"中国中医药优秀期刊"及"中国学术期刊优
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发布时间：2024-09-23 - 摘要：<正>《中国中药杂志》系中国科协主管,中国药学会主办,中国中医科学院中药研究所承办的综合性中药学术期刊。创刊于1955年7月,是创刊最早、发行量最大的中药学术刊物。《中国中药杂志》全面反映我国中医科研最高学术水平,主要报道该
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发布时间：2024-09-23 - 摘要：<正>优先数字出版就是以数字出版方式提前出版印刷版期刊内容。按照《中国实验方剂学杂志》与中国知网签订的优先数字出版协议,已经完成审稿及编排的、符合相关规定的稿件可以在印刷版出版前在中国知网优先数字出版平台上发布。发布内
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发布时间：2024-09-23 - 摘要：<正>尊敬的各位专家、医药界同行及各有关单位代表:为了推动中医方剂创新研究和开发,进一步加强国内中药复方研究领域的合作与交流,"首届全国方剂组成原理高峰论坛"定于2012年第三季度召开。届时将会有两院院士、主管部门的领导和国内外著名专家及相关知名企业参会。本次论坛由中国中西医结合学会中药专业委员会、中国实验方剂学杂志社、天津中医药杂志社共同主办,中国实验方剂学杂志社具体承办。欢迎从事中医方剂及其相关学术领域研究的同行积
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发布时间：2024-09-23 - 摘要：Objective:To study the difference of Alisma pieces which are processed in different way.Method:Fingerprints of Rhzoma alismatics orientalitis and its processed pieces are developed.The information of each processed Alisma pieces fingerprint is extracted with traditional Chinese medicine fingerprint similarity evaluation system evaluation system,and analyzed by SPASS.Result:The fingerprint of cach processed pieces Alisma pieces and raw Alisma pieces are significantly different,in which 14 peaks are characteristic peaks of difference Alisma pieces.Conclusion:The difference between characteristic components of processed Alisma pieces and raw Alisma pieces is significant.关键词：Alisma;the fingerprint of processed Pieces;analysis of components
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发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method of determining baicalein-7-O-gentiobioside and baicalin in Senmen oroxyli simultaneously.Method:The separation was performed at 30 ℃ on a Zorbax Extend-C18 column（4.6 mm × 250 mm,5 μm） with acetonitrile-methanol-0.3% H3PO4as the mobile phase.The flow ratewas 1 mL.min-1.The detection wavelength was at 280 nm.Result:The linear rage of baicalein-7-O-gentiobioside and baicalin was 0.061-1.525 μg（r=0.999 9） and 0.276-6.900 μg（r=1.000 0）,respectively.The average recoveries（n=6） of baicalein-7-O-gentiobioside and baicalin were 98.06% and 100.35%,respectively.Conclution:The established method can be used for quantitative analysis of baicalein-7-O-gentiobioside and baicalin in Senmen oroxyli,which can provide a reference for quality control of this traditional Chinese medicine.关键词：Senmen oroxyli;baicalein-7-O-gentiobioside;baicalin;HPLC;content determination
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发布时间：2024-09-23 - 摘要：Objective:To develop UPLC and a hydrophilic interaction chromatography（HILIC） method for the determination of trigonelline in Pinellia ternate.Method:After extracting trigonelline in the fine powder of Pinellia ternate by ultrasonic extraction with 50% methanol,the separation was performed at ACQUITY UPLC BEH HILIC column（2.1 mm × 100 mm,1.7 μm）.The mobile phase consisted of acetonitrile-10 mmol.L-1 ammonium acetate（80∶ 20）,and the flow rate was 0.4 mL.min-1.The detection wave length was set at 265 nm.The sampling volume was 10 μL.Result:The method has good linearity in the range of 1.125-72 nmol.mL-1 for trigonelline （r=0.999 6,n=7）.The average recovery was 99.7%（RSD 1.4%）.Conclusion:The method is specific,accurate and stable.It can be used for determination of trigonelline in Pinellia ternate.关键词：HILIC method;Pinellia ternate;trigonelline
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发布时间：2024-09-23 - 摘要：Objective:To analyze the chemical constituents of organic acids in Xiaoaiping injection.Method:The constituents of organic acids were separated by LC-MS,and then the contents was identified by HPLC.Result:Thirteen compounds was identified,representing 50.33% of the total organic acids.And the fragmentation behaviors of three organic acids were investigated.Conclusion:The principle chemical constituents of organic acids in xiaoaiping injecton are neochlorogenic acid（7.62%）,chlorogenic acid（10.56%）,cryptochlorogenic acid（4.62%） and p-coumaric acid（4.22%）.关键词：LC-MS;Xiaoaiping;organic acids
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发布时间：2024-09-23 - 摘要：Objective:A near-infrared（NIR） quantitative method was developed for rapid and nondestructive determination of moisture content in Danshen polyphenols acid injection.Method:The diffuse reflectance near-infrared spectra of Danshen polyphenols injection were measured intact by a fiber optic probe.The quantitative calibration model was developed using partial least squares（PLS） algorithm.Result:Using 53 samples Near-infrared spectra were pretreated with the second Derivative.The wavebands containing 11 993.1-6 098.8 cm-1 and 5 450.7-4 598.2 cm-1were selected,established and the quantitative calibration model of moisture content in Danshen polyphenols injection was established.The results shows that R2was 0.991 1,root mean square errors of Cross-Validation（RMSECV） was 0.210 and the best dimension was 4.10 samples as validation set,the RMSEP was 0.243.Conclusion:The presented method is timesaving,convenient and accuracy,which could be applied for rapidly determining moisture content in large numbers of samples.It is valuables for us to research the quality of traditional Chinese medical preparations.关键词：Danshen polyphenols injection;near-infrared spectroscopy;moisture content
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发布时间：2024-09-23 - 摘要：Objective:To establish the method of fingerprint analysis on Mahonia Forunei for controlling the quality.Method:The main components of the Mahonia Forunei from different sampes were determined by HPLC.Result:Ten common peaks were confirmed on the fingerprint,studying on the fingerprint of the Mahonia Fortunei.Conclusion:The method is reliable and accurate with good reproducibility and characteristic,providing a scientific base for controlling the quality of Mahonia Forunei by establishing fingerprint of the Mahonia Forunei.
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发布时间：2024-09-23 - 摘要：Objective:To study the antioxidant activity of Saururus chinensis in vitro.Method:The antioxidant activities of S.chinensis were evaluated by 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging and [2,2′-azino-bis（3-ethylbenzothiazoline）-6-sulphonic acid] diamonium salt（ABTS） radical scavenging.Result:n-Butanol extract from S.chinensis showed higher DPPH radical scavenging activity（IC50=16.94 mg·L-1） than that of BHT（IC50=18.71 mg·L-1） as positive control,and lower than that of PG and BHA（IC50=0.89,3.2 mg·L-1） as positive control.ABTS radical scavenging activity（IC50=12.90 mg·L-1） showed much lower than that of than PG and BHA（IC50= 0.81,1.95 mg·L-1） as positive controls,and slightly lower than that of BHT（IC50=7.72 mg·L-1）.Petroleum ether extract and ethyl acetate extract showed lower DPPH and ABTS radical scavenging activity than that of PG,BHT and BHA.Conclusion:n-Butanol extract from S.chinensis had antioxidant activity.关键词：Saururus chinensis;antioxidant activity;DPPH;ABTS
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发布时间：2024-09-23 - 摘要：Objective:To establish a method for the determination of polysaccharide in Lethariella cladonioides.Method:Using microplate reader,the content of total sugar was determined by means of phenol-sulfuric acid method and 3,5-dinitro salicylic acid（DNS） method was used for the determination of reducing sugar,the difference between the two was the polysaccharide content.Results:The total sugar standard curve equation was OD=0.051 4 C-0.014 6（r=0.999 2） and reducing sugar standard curve equation was OD =0.022 9C-0.086（r=0.999 9）.Respectively the linear range was 2.5-12.5 mg.L-1 and 8-40 mg.L-1.Thepolysaccharide content of LC,LCA,LCP were 63.6%,82.6%,57.6%.Conclusion:The method is fast,simple,sensitive,accurate and reproducible.关键词：红雪茶;总糖;还原糖;苯酚-硫酸法;3,S-二硝基水杨酸法
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- 713
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- 23
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for the determination chlorogenic acid and baicalin in Kunlun Chrysanthemum.Method:The HPLC method was used.The sample was analyzed on an Inertsil ODS-SP（4.6 mm × 150 mm,5 μm） column at 25 temperature,with mobile phase consisting of methanol and 0.05% phosphoric acid（pH 3.0） by gradient eluteion at the flow rate of 0.6 mL.min-1,The wavelength was at 330 nm.Result:The regression equation of chlorogenic acid was linear in the range of 0.45-15.00 mg.L-1,A =55 102C-24 808,r = 0.999 8（n = 6）.The average recovery was 102.58%（RSD 0.93%）.The regression equation of baicalin was linear in the range of 0.90-30.00 mg.L-1,A = 25 599C-16 099,r = 0.999 7（n = 6）.The average recovery was 104.90%,（RSD 1.12%）.Conclusion:The method was stable and accurate,it is suitable to determine chlorogenic acid and baicalin in Kunlun Chrysanthemum关键词：Kunlun Chrysanthemum;chlorogenic acid;baicalin;HPLC
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- 641
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- 49
发布时间：2024-09-23 - 摘要：Objective:Identification of matrine and ammothamnine in picrasmae lignum by high performance liqid chromatography /Tandem Mass spectrometry.Method:Two alkaloids in picrasmae lignum were ananlyzed by HPLC-ESI /Q-TOF MS /MS.According to the accurate molecular weight,retention time and MSn data by compared with reference substances,the chemical structures were identified.After that,quantitative method was built based on the techniques of LC-Q-Q-Q.Result:Two trace components were identified in picrasmae lignum.The method is specific,sensitive and can be used as the identification of matrine and oxymatrine in picrasmae lignum.Conclusion:It was the first time to report the identification of Matrine and ammothamnine in picrasmae lignum by LC-MS.The content of matrine and ammothamnine in picrasmae lignum vary greatly with the different part and the area of produce.关键词：picrasmae lignum;matrine;ammothamnine;HPLC-ESI/Q-TOF MS/MS;LC-Q-Q-Q
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- 467
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- 19
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method to determine arctiin in Jingju Ganmao tablets.Method:The determination was conducted by HPLC using a VARIAN Microsorb 100-5 C18 column.The mobile phase consisted of CH3OH∶ H2O（40∶ 60） with flow rate of 1.0 mL.min-1 and the detection wavelength was 280 nm.Result:This method had good resolution and good linear relationship between 0.042 8-0.856 g.L-1.The averagere covery of arctiin was 100.2% and RSD was 1.45%（n=6）.Conclusion:This method is simple,quick and accurate.It can be used for quantity control of Jingju Ganmao tablets.关键词：Jingju Ganmao tablet;Arctiin;HPLC
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- 80
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- 6
发布时间：2024-09-23 - 摘要：Objective:To develop an effective HPLC method based on a reaction of phenylisothiocyanate（PITC） with glucosamine（GL） in alkaline media for the determination of glucosamine hydrochloride in dosage forms.Method:Reverse phase chromatography using pre-column derivatization with phenylisothiocyanate,and ultraviolet detection（254 nm） was used to quantify the eluate.The reaction produces a phenylthiocarbamyl-glucosamine（PTC-GL） adduct which was separated on a reverse-phase（RP） column packed with Agilent TC C18（4.6 mm×250 mm,5 μm）.The mobile phase consisted of pH 5.8 phosphate buffered saline acetonitrile（90︰10） and was pumped at a flow rate of 0.8 mL·min-1,and the column temperature was maintained at 30 ℃.Galactosamine hydrochloride（Gal-HCl） was used as an internal standard.Result:The standard curves for GL-HCl showed linearity（r=0.999 1） over the selected concentration range from 350 to 550 mg·L-1 for dosage forms.The average recoveriy of glucosamine hydrochloride was 98.3%（RSD 2.3%）.Conclusion:The method was found to be specific and with excellent linearity,accuracy and precision and is well suited for the quantitation of GL-HCl in dosage forms.关键词：HPLC;pre-column derivatization;phenylisothiocyanate;glucosamine hydrochloride
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- 228
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- 4
发布时间：2024-09-23 - 摘要：Objective:To determine the content of atractylenolide Ⅱ in crude drugs and slices of Atractylodes macrocephala from different areas by HPLC.Method:The samples were separated on Tigerkin C18column（4.6 mm×200 mm,5 μm）with the mobile phase of methanol-water（77∶23）.Flow rate was 1.0 mL·min-1.The detection wavelength was set at 276 nm.Column temperature was maintained at 30 ℃.Result:The contents of atractylenolide Ⅱ in crude drugs from 10 places of origin and slices and processing slices of A.macrocephala from 20 regions was determined.Conclusion:The contents of atractylenolide Ⅱ in crude drugs and slices of A.macrocephala from different regions was different.关键词：atractylenolide Ⅱ;crude drugs and slices of Atractylodes macrocephala;content determination
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- 389
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- 15
发布时间：2024-09-23 - 摘要：目的:建立栀子金花丸中2个黄酮苷类成分的含量标准。方法:以HPLC法对栀子金花丸中的黄芩苷、汉黄芩苷进行定量分析。Kromasil C18柱（4.6 mm×250 mm,5μm）;流动相甲醇-0.3%冰醋酸溶液（43∶57）;检测波长275 nm。结果:黄芩苷、汉黄芩苷分别在0.105 5～2.110 0μg,0.036 2～0.724 0μg（r>0.999 8）线性关系良好,平均回收率大于97.50%,RSD小于1.95%。结论:该研究同时进行黄芩苷、汉黄芩苷的定量分析,可作为栀子金花丸的质量控制标准。关键词：栀子金花丸;黄芩苷;汉黄芩苷;含量测定;高效液相色谱法
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- 74
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- 2
发布时间：2024-09-23 - 摘要：Objective:To study on secondary metabolites from an endophytic fungus of Astragalus membranaceus.Method:The compounds were isolated by column chromatography and identified on the basis of physic-chemical constants and spectral analysis.Result:Six compounds were obtained and elucidated as cerevisterol（1）,ergosterol peroxide（2）,fumitremorgin B（3）,verruculogen（4）,fumitremorgin C（5） and cyclotryprostatins B（6）.Biological evaluation showed that the compounds displayed significantly inhibitory activities.Especially,compound 6 had strong antifungal activity.Conclusion:The six compounds were obtained from an endophytic fungus Aspergillus sp.of A.membranaceus for the first time.关键词：endophytic fungus;astragalus membranaceus;Aspergillus sp.;secondary metabolites;antimicrobial activity
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- 344
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- 26
发布时间：2024-09-23 - 摘要：Objective:The volatile oil and fatty acid of the Dioscorea cirrhosa.were analyzed.Method:In this study,the capillary GC-MS was used.Result:Fifty peaks were separated,12 compounds were identified from the fatty acids,the tuber of D.cirrhosa contained abundant octadecadienoic acid and hexadecanoic acid,they were 21.59% and 16.22%of total fatty acids respectively.And 32 peaks were separated and 32 compounds were identified from the volatile oil,m-cresol（26.92%）,phenyl hydroxide（18.95%）,o-cresylic acid（12.12%） were the dominant constituents.Conclusion:The results provide the scientific basis for comprehensive development and utilization of D.Cirrhosa.关键词：Dioscorea cirrhosa;fatty acid;volatile oil;GC-MS
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- 131
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- 6
发布时间：2024-09-23 - 摘要：Objective:To investigate the chemical constituents of the essential oil from the herb of Cymbopogon citratus lemongrass and provide useful information for the resonable utilization of lemongrass in Hainan.Method:The essential oil were extracted by water steam distillation,then analyzed by GC-MS and the irrelative contents were calculated by area normalization method.Result:Thirty-three peaks were separated by GC and 30 compounds were identified.The main constituents of the oil were geranial（25.36%）,neral（19.76%）,geraniol（12.39%）,geranyl acetate（4.06%） and β-myrcene（3.48%）.Conclusion:The main constituents of the essential oil from lemongrass in Hainan were monoterpenes and their oxygenated derivatives,which were approximately the same as those grown in other parts despite the small difference in contents.关键词：Cymbopogon citratus;essential oil;GC-MS;Hainan province
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- 460
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- 22
发布时间：2024-09-23 - 摘要：Objective:This study determined the amino acid,metal elements and arsenic in Gui Faeces Bombycis to discuss the nutritional composition and usage security.Method:The amino acid components and metal elements（Cu,Fe,Mn,Zn,K,Na,Mg,Ca） were analyzed by amino acid analyzer and flame atomic absorption spectrometry,respectively.Heavy metal elements（Pb,Cd） and harmful element（As） were determined by Graphite furnace atomic absorption spectrometry.Result:Sixteen amino acid components were contained in Gui Faeces Bombycis,the total content was 7.95%,in which aspartic content was 12.45%.10 metal elements such as Fe,Mn,etc.Were determined in it,especially the content of Ca was up to 34 000 μg·g-1.The content of heavy metal elements was as follows:Cu 8.2 μg·g-1,Pb 3.1 μg·g-1,Cd 0.088 μg·g-1.The harmful element（As） was 0.28 μg·g-1 and much lower than the specified value.Conclusion:Gui Faeces Bombycis was rich in amino acids compositions and metal elements,however,its heavy metal and harmful elements were in the safe range.It could be used as raw materials of health products and drugs due to its safe and effective character and abundant nutritional value.关键词：Gui Faeces Bombycis;amino acids;metal elements;arsenic
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- 154
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- 14
发布时间：2024-09-23 - 摘要：Objective:To establish an effective method for identification of main species of Acanthopanax in Tibetan and Qiang Ethnic area of Sichuan by HPLC.Method:The analysis was carried out at 30 ℃ on a Kromasil C18 column eluted with a mobile phase consisted of methanol-0.5% phosphoric acid,and the data were analyzed with‘Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica’software.Result:The established chromatographic fingerprint method was repeatability,among which 12 fingerprint peaks in common were confirmed.The DPSv3.0 statistics software was used for clustering analysis with the clustering method of Betweengroups linkage and Euclidean distance.The analysis result was that S1,S2 and S3 of the different medical part of A.giraldii Harms were mostly similar,then that of S5,S4,S8,S6 and S7 in turn.Conclusion:This chromatographic fingerprint could be used specifically to distinguish the main species of Acanthopanax Miq.in Tibetan and Qiang Ethnic area of Sichuan.关键词：Acanthopanax giraldii;A.setchuenensis;A.leucorrhigus var.fulvescens;HPLCfingerprint;identification
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- 137
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- 3
发布时间：2024-09-23 - 摘要：Objective:To study on effect of borneol（natural or synthetic）,synthetic musk and total prescription of Xingnaojing on nasal absorption of gardenia extract.Method:After combined with different components,nasal absorption in rats of geniposide was investigated with modified in situ nasal perfusion model by volume correction method.Result:Absorption coefficients（K） of geniposide（the content of it were 36.8,92,368 mg·L-1,respectively） in gardenia extract solution（concentration were 100,250,1 000 mg·L-1） were（2.15 ± 0.70）×10-3,（1.97 ± 0.48）×10-3,（1.87 ± 0.81）×10-3 min-1,respectively.Nasal absorption of geniposide in gardenia extract fitted first-order rate process and could be considered as passive diffusion absorption.When gardenia extract combined with borneol（natural or synthetic）,synthetic musk and in total prescription of Xingnaojing,K value of geniposide was 1.4,1.7,1.1,1.3 times to it of gardenia extract alone,respectively.Correlation between in situ nasal absorption coefficients and in vitro mucosa permeability coefficients was good,KA=0.220 6 KI+0.559（r =0.991 0）.Conclusion:Nasal absorption of geniposide could be remarkably increased in Xingnaojing prescription and borneol played a major role in promoting absorption in this prescription.关键词：Xingnaojing;gardenia extract;in situ rat nasal perfusion;synthetic borneol;synthetic musk
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- 348
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- 16
发布时间：2024-09-23 - 摘要：Objective:To study the absorption kinetics of the active components of Tiangou Jangya capsules in rats’ intestinal.Method:Using an improved rat everted gut sac model,gastrodin concentration and paeonol concentration were determined by HPLC.The concentration of cumulative absorption was calculated.The linear regression equation was figured through the concentration of cumulative absorption and time.The parameters were calculated.Result:The regression equation of gastrodin was Y=0.000 6X-0.012 8（r=0.993 0）,Ka=6.0×10-5 μg·min-1·cm-2,t1/2=1.16×104 h;the regression equation of paeonol was Y=0.000 68X+0.001 1（r=0.983 5）,Ka=6.8×10-5 μg·min-1·cm-2,t1/2=1.02×104 h.Conclusion:The absorption of gastrodin and paeonol conforms to the first-order kinetics in rats’intestinal.关键词：Tiangou Jiangya capsule;gastrodin;paeonol;Absorption Kinetics;HPLC
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- 205
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- 6
发布时间：2024-09-23 - 摘要：Objective:To investigate the protective effects and mechanism of oxymatrine（OMT） on experimental acute myocardial infarction（AMI） in rats induced by ligatting left anterior descending branch of coronary artery.Method:The SD rats were allotted into 4 groups according to the body weight as following:sham operation,model group,the OMT 50 mg·kg-1and 25 mg·kg-1 groups,the rats were orally administered for 5 days.The rat AMI model was reproduced by ligation of left anterior descending branch of coronary artery after 1 hour of the last treatment,the ligation of the artery did not be performed in sham operation group.The sample was collected after ligation of left anterior descending branch of coronary artery for 6 hours.The pathological changes of cardiac tissue were checked by HE staining.The indexes of oxidative stress were detected by biochemical assay kits,including the total antioxidant capacity（T-AOC）,activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px）,and malondialdedyde（MDA） content.And the contents of the interleutin-1β（IL-1β）,interleutin-6（IL-6）,and tumor necrosis factor-α（TNF-α） in serum were measured by Elisa kits.Result:The histological examination indicated there was a myocardial lesion in model group including edema of myocardial interstitial and inflammatory cell infiltration,etc,after ligation of left anterior descending branch of coronary artery,OMT 50 mg·kg-1 could ameliorate the histopathological changes.The activities of antioxidant enzymes in serum were significantly decreased such as SOD,CAT,and GSH-Px,as well as MDA contents in serum were increased,and the contents of IL-1β,IL-6,and TNF-α in serum were increased in model group（compared with sham group,there were significant difference,P<0.01 or P<0.05）.50 mg·kg-1 OMT could obviously ameliorate oxidative stress and inflammatory cytokines in serum（compared with model group,P<0.01 or P<0.05）.Conclusion:OMT can protect the cardiac injury in rats induced by ligating left anterior descending branch of coronary artery,the mechanism maybe involve in inhibiting secretion of inflammatory cytokines and ameliorating oxidative stress.关键词：oxymatrine;myocardial infarction;oxidative stress;inflammatory cytokines
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- 313
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- 24
发布时间：2024-09-23 - 摘要：Objective:To study the influence of polygonum cuspidatum anthraquinone（PCA） on transforming growth factor-petal（TGF-β1） and Smad3 levels in cortex renisin mice diabetic nephropathy（DN） model.Method:DN mouse were induced by STZ and randomized into high,middle and low close of PCA groups,positive control group and model group with 17 mousein each group.Another 17 mouse were chosen as normal control.The levels of PCA on TGF-β1 and Smad3 in cortex renis were assayed by immunohistochemistry method.Result:TGF-β1 and Smad3 levels in cortex renis from DN model were significantly increased,and Smad3 levels in cortex renis from middle close of PCA groups was significantly decreased.There was a downward tendency of TGF-β1 and Smad3 levels in cortex renis from high,middle and low dose of PCA groups,but no statistical significance.Conclusion:TGF-β1and Smad3 may involve in the occurrence of DN;PCA can inhibit Smad3 expression in cortex ren is from DN model.
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- 565
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- 14
发布时间：2024-09-23 - 摘要：Objective:To explore The effect of modified Jiawei Xiaoyao Tang on cyclic adenosine monophosphate（cAMP）,protein kinase A（PKA） and protein kinase C（PKC） in rat hippocampus with depression.Method:Rats were randomly divided into six groups:control group,model group,clomipramine group（13.5 mg·kg-1）,modified Jiawei Xiaoyao Tang of high,medium and low dose group（11.7,5.85,2.925 g·kg-1）.Each group was administrated continuously for 24 ds.After 1 hour of administration,the hippocampus was separated rapidly into the freezing tube and placed in-70 ℃ refrigerator to detecte the level of cAMP,PKA and PKC by ELISA.Result:Compared with model group,each group of modified Jiawei Xiaoyao Tang significantly increased level of cAMP,PKA and PKC（P<0.05） with dose-depandent manner.Compared with clomipramine group,high and medium dose showed the no significant differences.Conclusion:The anti-depression effect of modified Jiawei Xiaoyao Tang may be related to regulating the signal transduction of cAMP,PKA and PKC.关键词：modified Jiawei Xiaoyao Tang;depression;cyclic adenosine monophosphate;protein kinase A;protein kinase C
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- 360
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- 8
发布时间：2024-09-23 - 摘要：Objective:To investigate the bioadhesion and the purgation effect of colon-specific microspheres of total anthraquinones in Rhei Radix et Rhizoma.Method:Observing the percentage of bioadhesive of microspheres in different pH solutions with in vitro perfusion technique and at different intestines part with in vivo perfusion technique to evaluate the bioadhesiveness of colon-specific microspheres of total anthraquinones in Rhei Radix et Rhizoma.With passage of loose stools time,loose stools amount and in 8 h amount of stools as investigation index to investigate the purgation effect of total anthraquinones in Rhei Radix et Rhizoma colon-specific microspheres.Result:The microspheres adhered to the intestines at different pH environment with in vitro perfusion technique.The percentage of bioadhesive was 79%-68% from pH 3.5 to pH 7.0.The microspheres adhered to the stomach,small intestine and colon using in vivo perfusion were 8.5%,23%,63%.The 8 h stools amount of mice lavaging microspheres（0.2 g·kg-1） were（66.4±8.4）pills,the weight of stools in 8 h was（0.74±0.41） g.There was no significant difference between the microspheres and Rhei Radix et Rhizoma（4.0 g·kg-1） on purgation effect.Conclusion:The colon-specific microspheres of total anthraquinones in Rhei Radix et Rhizoma.had correspond purgation effect with equal doses of medicine and the bioadhesive percent was good.关键词：total anthraquinones in Rhei Radix et Rhizoma;colon-specific;bioadhesion;microspheres;purgation effect
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- 442
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- 20
发布时间：2024-09-23 - 摘要：Objective:To study the diuetic effects and its mechanism of Guiling Shenzhu decoction and its different effective parts.Method:The effect on urinre volum and urinary electrolyte（Na+,K+,Cl-） were assessed by using rat metabolic cage and electrolyte analyzer following oral administration of Guiling Shenzhu decoction（10.0,5.0,2.5 g·kg-1） and its different polar fractions（10,5.0,2.5 g·kg-1）.Result:Guiling Shenzhu decoction（5.0,2.5 g·kg-1） and extraction of ethyl acetate（5.0 g·kg-1） and residual water layer（5.0 g·kg-1） had good diuretic effect.The urine K+ excretion increased significantly,but Na+ and Cl-excretion did not increase.Conclusion:Guiling Shenzhu decoction and extraction of ethyl acetate and residual water layer have significant and persistent diuretic effect.They cause marked increase in urinary K+level and it may be the diuetic mechanism.关键词：Guiling Shenzhu decoction;effective parts;diuretic activity
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- 144
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- 6
发布时间：2024-09-23 - 摘要：Objective:The present study was designed to use cultured neonatal rat cardiomyocytes with hypoxia-reoxygenation（H/R） to mimic in vivo ischemia-reperfusion injury（I/R）,and investigate the protective mechanism of fuzi polymccharide in cardiomyocytes against hypoxia-reoxygenation injury through metallothionein.Method:Cultured rat myocardial cells were divided into four groups:control,H/R,and fuzi polymccharide treatment groups.The cells in H/R group were incubated primarily in hypoxic buffer solution for 3 hours,thereafter,these cells were incubated for 6 hours in normal culture medium.The cells in fuzi polymccharide treatment group were cultured in medium added with 10.0,1.0,0.1 g·L-1 fuzi polymccharide for 24 hours before H/R.metallothionein of cardiomyocytes was measured by ELIZA,cell apoptosis of cardiomyocytes was measured by flow cytometry,MDA of cardiomyocytes and LDH in cell culture media were also measured.Result:As compared with H/R group,pretreatment with high and moderate dose of fuzi polymccharide increased the synthesis of metallothionein,decreased the synthesis of MDA and release of LDH.High dose of fuzi polymccharide decreased cardiomyocyte apoptosis.Conclusion:These data suggest that fuzi polymccharide pretreatment can protect cardiomyocytes from H/R injury.The mechanism is related to its enhancement of metallothionein synthesis,scavenging of oxygen free radical,and thus protection against apoptosis.关键词：fuzi polymccharide;metallothionein;hypoxia-reoxygenation injury
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- 217
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- 23
发布时间：2024-09-23 - 摘要：Objective:To explore the mechanism of Qiaoqing Qingfeiji to relieving asthma of asthma model by studying the effect of Qiaoqin Qingfeiji on relieving asthma and the relation between it and the content of platelet-activating factor（PAF）,white blood cell（WBC）,eosinophils（EOS） and endothelin（ET） of asthma model.Method:Rats were divided into 6 groups:normal control group,asthmatic model group,Qiaoqin Qingfeiji with hight,meddle,and low dosage groups,and dexamethasone group,and aminophylline group.Asthmatic model was established by sensitizing and challenging rats with ovalbumin（OVA）.Except the normal control group,other groups were injected 10% OVA into abdominal cavities in the 1th day and the 8th day.2 weeks later followed by aerosolized 1% OVA once a day challenge to prepare the asthma models for 2 weeks.The drugs for every group were given intragastrically from the second day of rat sensitization for 4 weeks（the dexamethasone was given from the 1th day）.The effect of medicine on the asthma degree,asthma latency period and the content of PAF,WBC and EOS in serum,and ET in bronchoalveolar lavage fluid（BALF） of asthma model were observed.Result:Qiaoqin Qingfeiji can obviously improved the asthma degree,and extend asthma latency period（P<0.05）,and obviously decrease the content of PAF,WBC and ET（P<0.05-0.01）.Conclusion:Qiaoqin Qingfeiji can effectively recover from status asthmaticus,reduce the severity of asthma attacks.It is relate to reduce the content of PAF and WBC in serum,and ET in BALF of asthma model.关键词：Qiaoqin Qingfeiji;asthma;platelet-activating factor;endothelin;eosinophils
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- 88
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- 11
发布时间：2024-09-23 - 摘要：Objective:To observe the protective effects of Shuangge Jiecheng Pill on ferments alcoholic fatty liver in rats.Method:The rat alcoholic fatty liver model was induced by 56%Hongxing wine by intragastric administration with 10 mL·kg-1,twice per day for 8 weeks.Then,the successfully modeled rats were successively administrated for 4 weeks,and randomly divided into model group,HW group（Haiwang Jinzun tablet,1.5 g·kg-1）and Shuangge Jiecheng pill（SG） group with high dose（HD）,middle dose（MD） and low dose（LD）（crude drug 20,10,5 g·kg-1·d-1）,and the control group was set up in the same period.After administration and fasting for 12 h later,the blood was obtained from arteria cruralis for detecting theactivity of γ-glutamy（transferaseγ-GT）,alanine aminotransferase（ALT） and aspartate aminotrans-ferase（AST）,and the liver index was observed also.Result:Compared with the control group,avtivity of γ-glutamyltransferase（γ-GT）,alanine aminotransferase（ALT） and aspartate aminotransferase（AST） and the liver indexes in the model group were obviously increased（P<0.05） while the body weight was decreased（P<0.01）.Compared with the model group,activity of γ-GT,ALT and AST and the liver indexes in the HW and SG group of HD,MD and LD were obviously decreased（P<0.05,P<0.01） while the body weight was increased（P<0.01）,and the indexes in SG group of HD were obviously decreased（P<0.05,P<0.01） than those of SG group of MD and LD.Conclusion:Shuangge Jiecheng Pill has an protective actions on for alcoholic fatty liver.关键词：alcoholic fatty liver;γ-glutamyltransferase;alanine aminotransferase;aspartate aminotransferase
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- 156
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- 4
发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Jiejiu recipes of Gegen powder on the cytochrome P450（CYP450） content of liver microsomal and the activity CYP2E1 in mice acute alcohol-induced liver injury model.Method:Mouse were divided into normal control group,model group and Gegen powder high,medium nad low dose groups.Gegen powder of 20,10,5 g·kg-1,was given once a day and continuing ten days.After 30 minutes of administration everyday,the mouse of model group and treatment groups were orally given 56% alcohol of 15 mL·kg-1 to establish acute alcohol-induced liver injury model,and the level of ALT,AST in serum and liver index were observed;Content of CYP450 and the activity of CYP2E1 were detected.Result:Compared with normal control group,the level of ALT,AST and liver index in model group had obvious enhance（P<0.01）,the content of microsomal protein had no significant change,the content of CYP450 and the activity of CYP2E1 were significantly raised（P<0.01）.Compared with model group,the level of ALT,AST and liver index in Gegen powder groups had obvious reduction（P<0.05 or P<0.01）,the content of microsomal protein had no significant change,the content of CYP450 was increased significantly（P<0.01）,the activity of CYP2E1 was significantly decreased（P<0.01） and there was a significant difference between different dosage groups（P<0.05）.Conclusion:Gegen powder indicates the therapeutic effect on acute alcohol-induced liver injury,it can be contribute to increaseing the content of CYP450 and reduceing the activity of CYP2E1关键词：Gegen powder;acute alcohol-induced liver injury;CYP450;CYP2E1
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- 1062
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发布时间：2024-09-23 - 摘要：Objective:To study the anti-alcohol effecty of Xiangsi Tengcha（XSTC）.Method:Before alcoholism,mouse were given XSTC（16,8,4 g·kg-1）,haiwang jinzun tablets（HWJZT 1.5 g·kg-1） and saline for six days.After 30 minutes of the last administration,the mouse were given with 56% alcohol every 20 minutes until they died.The drunk（righting areflexia） incubation period,dose of alcohol intake,time of death and lethal dose of alcohol were recorded.The activity of AST,ALT,ADH,ALDH,SOD and the content of MDA were determined.Result:The incubation period of drunkenness was extended significantly.The activity of ADH,ALDH and SOD were increased and the activity of ALT,AST and the content of MDA were decreased（P<0.05）.Conclusion:XSTC could prevent drunkenness caused by strengthened the activity of ADH,ALDH and SOD.
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- 420
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of active part of spatholobus suberectus flavonoid（SSCE） on oxidative stress in human pulmonary adenocarcinoma cell A549.Method:Cells were divided into a high-dose group,a moderate-dose,a low-dose group and a normal control group.Cell A549 were interfered with different dosages of SSCE active part for 24 h（normal group with DMEM） level of malonic dialde hyde（MDA） and glutathione（GSH）;reactive oxygen speiesROS was detected using fluorescence microplate reader and confocal fluorescence microscope,and using DCFH-DA as fluorescent probe;Antioxidant was added,CCK-8 was employed to detect cell proliferation of A549 and ROS was detected by fluorescence microplate reader.Result:After the addition of SSCE,MDA content in each group increased（P<0.01） and GSH content in high density group decreased（P<0.01）;Cell ROS content in high and middle density groups increased（P<0.01）;Each group showed green fluorescence under confocal microscope,in which the high and middle density group showed higher;Addition of antioxidant made decrease of cell inhibition（P<0.05） and ROS content（P<0.01）.Conclusion:SSCE can change the level of A549 MDA,GSH and ROS,and its mechanism of antitumor might be inducing oxidative stress reaction of tumor cells.关键词：spatholobus suberectus flavonoids（SSCE）;reactive oxygen speies;oxidative stress;A549 cell
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- 435
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发布时间：2024-09-23 - 摘要：Objective:To investigate the neuron protective effect of Qingre Huayu prescription（QRHYP） preconditioning on focal cerebral ischemia/reperfusion（I/R） injury in rats.Method:160 male SD rats were randomly divided into four groups as following:sham-operation group,middle cerebral artery occlusion（MCAO） group,brain ischemia preconditioning（BIP） group and QRHY group.Each group was further divided into 4 subgroups according to 12 h,1 d,2 d and 3 d after I/R.The BIP model was established The pathomorphology of brain tissue,the apoptosis rate of neuron and infarct volume after I/R were evaliated.Result:①Light microscopy and electron microscopy showed that the change of pathomorphology in BIP group was more significantly improved than that in MCAO group.The infarct size of the BIP group was smaller than that of the MCAO group（P<0.05）.QRHYP treatment could very markedly reduce those injury compared with BIP group（P<0.05）.②The rate of apoptosis in MCAO increased markedly at 12 h after reperfusion than that of the sham group（P<0.01）,and reached the peak at 1 d,then decreased continuously,but still higher than sham group（P<0.01）.BIP could decrease the rate of apoptosis（P<0.05 or P<0.01）.QRHYP treatment could further lower apoptosis compared with BIP group（P<0.05 or P<0.01）.Conclusion:Qingre Huayu prescription precondtioning could have a synergistic neuron protective effect on I/R injury in rats.关键词：Qingre Huayu prescription;ischemia preconditioning;ischemia/reperfusion（I/R） injury;neuron protection
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- 250
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- 8
发布时间：2024-09-23 - 摘要：Objective:To exploe effect of Anwei pill on inflammatory cytokines of serum and the expression of nelclear factor-kappa B（NF-κB） p65 mRNA on gastric mucosa in chronic gastritis rats rats.Method:Rats chronic gastritis model was established by combined administration of 60% ethanol,20 mmol·L-1sodium deoxycholate and 0.05% ammonia water.Aanwei pills was given for 30 day.Levels of interleukin（IL-2）,（IL-4）,tumor necrosis factor-α（TNF-α） in serum were measured by enzyme-linked immunosorbent assay（ELISA）.The expression of NF-κB p65 mRNA in gastric mucosa was detected in situ hybridization.Result:The concentration of IL-4 and IL-2 in Anwei pill group was increased and decreased compared with model group,respectively.TNF-α was decreased.Anwei pill could decrease expression of NF-κB p65 mRNA in gastric mucosa.Conclusion:Anwei pill can protect gastric mucosa and inhibit the inflammation reaction.Its mechanism is probably associated with inhibition of the expression of NF-κB p65 mRNA and regulation of immunity.关键词：Anwei pills;chronic gastritis;interleukin;tumor necrosis factor-α;NF-kappa B
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- 181
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发布时间：2024-09-23 - 摘要：Objective:To study the anti-inflammatory,antalgic effects and acute toxicity of Ziziphora clinopodioides Lam.Method:The inhibition of auricle edema induced by xylene and paw edema induced by formaldehyde were used to test the anti-inflammation,pain caused by acetic acid in mice was used to evaluate the antalgic effect.Oral administration was used for acute toxicity tests.Result:Z.clinopodioides of low,medium and high dose groups on xylene in rats induced by Auricular swelling,foot swelling in mice induced by formaldehyde and acetic acid twisting had a significant inhibition in mice induced（P<0.05）.All mouse were survived,and no toxic reaction was found in the observed period.Conclusion:Z.clinopodioides has obvious anti-inflammatory and antalgic effects.The clinical dosage of the tablets is within the safty range.关键词：Ziziphora clinopodioides;anti-inflammation;analgesia;acute toxicity
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- 257
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发布时间：2024-09-23 - 摘要：Objective:To study the mechanism on treatment of metrorrhagia due to blood-heat by Kangyan Gongle capsule.Method:Female ICR mouse were randomized into model group,control group,Kangyan Gongle capsule groups treated with dose of 1.2,0.6,0.3 g·kg-1,Gongxuening group and Ibuprofen group,treatment lasted 5,10 and 10 days,respectively.Female SD rats were randomized into the groups as the above mentioned.The dose of Kangyan Gongle capsule was 0.94,0.47,0.24 g·kg-1 accordingly.The treatments（ig） lasted 20,7,30 days,respectively.Bleeding time,hemorheology,platelet aggregation rate,regulating gonadotrophin and gonadal hormone,anti-inflammatory and analgesic effect were determined to study the mechanism.Result:Kangyan Gongle capsule could obviously shorten the bleeding time of mice broken tail（P<0.05）,shorten prothrombin time（PT） and activated partial thromboplastin time（APTT）（P<0.05）,enhance platelet aggregation rate（P<0.05）,decrease the degree of mice ear edema caused by xylene（P<0.05） and rat uterus swelling by embedded foreign matter（P<0.05）,relieve mice’ pain caused by oxytocin（P<0.05）,upregulate the level of follide stimulating hormone（FSH）（P<0.05）,luteinizing hormone（LH）（P<0.05）,estradiol（E2）（P<0.05） and downregulate the level of P（P<0.05） in ovariectomized rat’ serum.Conclusion:Kangyan Gongle capsule obviously indicates hemostatic,antiinflammatory,analgesic effect and could regulate the function of hypothalamus-pituitary-gonad axis,which.May be included into the mechanism of actions.关键词：Kangyan Gongle capsule;metrorrhagia due to blood-heat;hemostasis;antiinflammatory;analgesic;gonadal axis
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- 139
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发布时间：2024-09-23 - 摘要：Objective:We aimed to establish a model to select dipeptidyl peptidase IV（DPP-IV）,and to test the inhibitory rate of the seven compounds.Method:DPP-IV was extracted from cultured Caco-2 cells and the values of absorbance（A） were tested in different activity units which were to evaluate the relation between the activity of DPP-IV and the value of A by the curve of activity-A.Sitagliptin as a positive control was used to evaluate the selected model.The derivatives from sitagliptin and the compounds were estimated inhibitory activity by the model.Result:The activity of DPP-IV was correlated to the value of A in a certain range.The IC50 values of sitagliptin,JD-1 and JD-2 were 18.354 nmol·L-1,3.4 μmol·L-1 and 2.6 μmol·L-1,respectively;the range of inhibitory activity for the compounds was between 21.45% and 27.77%.Conclusion:The IC50 of sitagliptin tested by this selected model is close to the value reported,which proves availability of the model.and meanwhile,the compounds estimated in this study contain inhibitory effect.关键词：model of dipeptidyl peptidase IV inhibitor;IC50;inhibitory rate
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- 378
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Jiangzhi granule（JZG） on hyperlipemia models.Method:Hyperlipemia models were established in mice,rat and quails.All animals were randomly divided into normal group,model group,xuezhikang group,JZG high,middle and low dose group.The dosage for mice,rat and quails was 10.0,5.0,2.5 g·kg-1,8.0,4.0,2.0 g·kg-1 and 8.0,4.0,2.0 g·kg-1,respectively Administration course was one week for mouse,four weeks for rats and 12 weeks for quails.The content of cholestero（CHO）,triglyceride（TG）,high density lipoprotein cholesterol（HDL-C） and low density lipoprotein cholesterd（LDL-C） in serum was determined after administration The atherosclerotic plaque in quail hyperlipemia models was exmined by histopathologic examination.Result:The content of CHO,TG,LDL-C in serum of mice,rats and quails was decreased remarkedly.The decrease of HDL-C/CHO index was reversed.Atheroselerotic plaque in quail lightened obviously.No change of HDL-C and VLDL-C were not found.Conclusion:JZG can effectively decrease the serum lipid and prevent the formation of atherosclerosis.关键词：Jiangzhi granule（JZG）;cholesterol;triglycerides;atherosclerosis
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- 162
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发布时间：2024-09-23 - 摘要：Objective:To study the protective mechanism of acanthopanax on learning and memory and monoamine neurotransmitters acetylcholinesterase（AchE）,serotonin（5-HT）,norepinephrine（NE）,5-hydroxindole acetic acid（5-HIAA） of hippocampus in sleep deprivation rats.Method:Total of 75 male wistar rats were randomized into 5 groups,large platform group,sleep deprivation group,three treatment groups（high,middle and low dose）.Treatment groups were given Acanthopanax solution（11.2,5.6,2.8 g·kg-1） for 7 days.The others were given distilled water.A small platform was used to establish rapid eye movement sleep（REMS） deprivation model in rats.After 4 days,the ability of learning memory and space exploration was tested by Hexagonal maze in rats.The level of AchE,5-HT,NE,5-HIAA in hippocampus homogenate were measured.Result:Compared with a large platform,the number of errors of sleep deprivation group increased,the rate of cognitive reduced（P<0.01）,but finding time was shorten（P<0.05）.After administration of Acanthopanax solution,the number of errors reduced significantly,the rate of cognitive increased（P<0.01）,finding time was shorten（P<0.01）,the level of 5-HT,NE.5-HIAA was higher in Acanthopanax solution than sleep deprivation group（P<0.05 or P<0.01）.The AchE level in treatment groups was lower thanthat in sleep deprivation group but higher than large platform group（P<0.05 or P<0.01）.Conclusion:Acanthopanax can improve learning and memory ablity in sleep deprived rats,and the mechanism may involve regulating the sleep deprivation caused by disorders of monoamine neurotransmitter,accelerating the metabolic conversion of 5-HT,regulating NE,and AchE activity in the hippocampus.关键词：Acanthopanax;sleep deprivation;monoamine neurotransmitters
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- 1121
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Zhuangshen Yihao granule on proteinuria excreting in nephrosis rats induced by adriamycin（ADR）,and discuss the mechanism in biochemistry and molecular biology.Method:The proteinuria model of nephrosis rats was established by injecting ADR（0.006 g·kg-1） by the tail vein.And then they were randomly divided into groups as follows:model group,lotensin group（0.003 g·kg-1）,low-dose group（2.52 g·kg-1） and high-dose group（10.08 g·kg-1） of Zhuangshen Yihao granule,and normal group was set up meanwhile,drugs were given accordingly once a day after 7 days of modeling,administration lasted 8 weeks.At the 8th week of he experiment,24 hours urinary protein was assayed,urine β2-MG and tumor necrosis factor（TNF）-αin blood serum were detected by radioimmunoassay.Result:Levels of 24 hours urinary protein,urine β2-MG and the content of TNF-α of serum in lotensin group,the low-dose and the high-dose groups of Zhuangshen Yihao granule were reduced,especially in the high-dose group of Zhuangshen Yihao granule（P<0.05）.Conclusion:Zhuangshen Yihao granule can exerted a protective effect on glomerulus and renal tubule,and revealed that producing an effect on the regulation network of cytokines,and abating inflammatory reaction may be its possible mechanism.Furthermore,the above curative effects of Zhuangshen Yihao granule in the high-dose group were better than in the low-dose group and the lotensin group.关键词：Zhuangshen Yihao granule;nephrosis induced by adriamycin;proteinuria
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- 96
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- 5
发布时间：2024-09-23 - 摘要：Objective:To investigate the controlling effects of Neiyi Fang on expression of mRNA and protein of（TGF-β1） in endometriosis（EMS） lesions of model rats.Method:The model of EMS was duplicated in SD rats,rats were divided into sham group,model group,low-dose of Neiyi Fang group（25.2 g·kg-1·d-1）,high-dose of Neiyi Fang group（50.4 g·kg-1·d-1） and western medicine（gestrinone） group（2.5 mg·kg-1·d-1）.RT-PCR was used to detect the expression of mRNA of TGF-β1 in EMS lesions.Western blot was used to detect the expression of protein of TGF-β1.Result:The level of expression of mRNA and protein of TGF-β1 in the model group was clearly higher than that in the sham group（P<0.01）,while in the Neiyi Fang group,the level was clearly lower than that in the model group（P<0.01）.The level in the Neiyi Fang group was no significant difference than in the western medicine group.Conclusion:The expression of mRNA and protein of TGF-β1 in EMS lesions of model rats were increased.Neiyi Fang resisted EMS through decreasing the expression of mRNA and protein of TGF-β1.关键词：model of EMS;TGF-β1;Neiyi Fang
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- 112
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发布时间：2024-09-23 - 摘要：目的:研究蒙药忠伦阿汤对胶原诱导性关节炎大鼠模型血液流变性的影响。方法:建立Ⅱ型胶原诱导性关节炎大鼠动物模型,设空白组、模型组、阳性药雷公藤多苷（0.02 g.kg-1）、蒙药忠伦阿汤低、中、高剂量（分别含生药1.8,0.9,0.45g.kg-1）组,ig 6周后,大鼠麻醉后断头取血,抗凝管收集4 mL全血,进行全血黏度、全血还原黏度、血浆黏度、纤维蛋白原和红细胞压积等相关指标的检测,观察蒙药忠伦阿汤对关节炎模型大鼠的血液流变性的影响。结果:忠伦阿汤各剂量对模型大鼠全血黏度、全血还原黏度有明显降低作用（P<0.05或P<0.01）,中、高剂量降低红细胞压积、纤维蛋白原含量和红细胞刚性指数（P<0.05或P<0.01）,高剂量降低红细胞聚集指数（P<0.05）。结论:蒙药忠伦阿汤可明显降低血液流变学各项指标,说明忠伦阿汤具有一定的活血化瘀作用。关键词：蒙药忠伦阿汤;血液流变性;胶原诱导性关节炎
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- 138
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发布时间：2024-09-23 - 摘要：Objective:To sudy the effect of Salvia miltiorrhiza extract on heart rate and myocardial enzyme after myocardial ischemia/reperfusion injury in rats.Method:The SD rats were randomly divided into six groups（n=8,each）:sham-operation group,model group,S.miltiorrhiza extract 12.8,6.3,3.2 mg·kg-1 groups and S.miltiorrhiza injection 4×103 mg·kg-1 groups.The myocardial ischemia/reperfusion injury animal model was established by ligaturing left ventricular branch of coronary artery for 30 min and reperfusion for 30 min.The S.miltiorrhiza extract at 12.8,6.4,3.2 mg·kg-1and S.miltiorrhiza injection at crude drug 4.0 g·kg-1were injected by femoral vein after 25 min myocardial ischemia and before 5 min reperfusion,and observing the changes of heart rate and myocardial enzyme.Result:S.miltiorrhiza extract significantly inhibited the decrease of heart rate and reduced serum aspartate transaminase（AST）,lactate dehydrogenase（LDH） and creatine kinase（CK） activity after myocardial ischemia/reperfusion injury in rats.Conclusion:S.miltiorrhiza extract can protect myocardial ischemia/reperfusion injury.关键词：Salria miltiorrhiza extract;myocardial ischemia/reperfusion injury;heart rate;myocardial enzyme
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- 321
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发布时间：2024-09-23 - 摘要：Objective:To observe effect of Baoji Wan powder on diarrhea and intestinal motility induced by irradiation in mice.Method:Using castor oil induced diarrhea model,we observed Baoji Wan powder’s（before and after irradiation） antidiarrheal effect at doses of 1.3,2.6,5.2 g·kg-1ig for 3 d in mice.Using pyridostigmine bromide induced hyperthyroidism intestine movement in mice,the effect of Baoji Wan powder on intestine movements was observed.Result:Baoji Wan powder before and after irradiation could significantly extend the castor oil-induced small diarrhea,the starting time of diarrbea（indicated by watery feces）,the number of diarrhea,compared with the control group（P<0.01）.The powder could significantly inhibit pyridostigmine bromide-induced intestinal motility compared with that in the model group（P<0.01,P<0.05）;and it could significantly reduce the water content in colon and small intestine,compared with that in normal control group（P<0.01,P<0.05）.Conclusion:Baoji Wan powder before and after irradiation has the effect of inhibiton of diarrhea and suppression of bowel movement,the antidiarrheal effect may be gained by inhibition of bowel movements and reduce of water in intestine.Before and after irradiation,Baoji Wan powder’s difference in effects was not significant.关键词：Baoji Wan;irradiation;diarrhea;intestine movement
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- 226
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发布时间：2024-09-23 - 摘要：Objective:To study the hepatotoxicity of Farfarae Flos.Method:KM mice were given the water extracts of Farfarae Flos orally in 20,40 g·kg-1 doses for successive 4 weeks,enzyme activity of aspartate transaminase（AST）,alanine transaminase（ALT） in serum of mice were measured,coefficient of liver were calculated,and liver histopathology examination were conducted.Using the precision-cut slice technology,culture liver slices for 24 hours with water extracts in concentration 0.005,0.05 g·L-1 and 6 hours with the total alkaloid of Farfarae Flos in concentration 0.5,2.0 g·L-1,the slice homogenate were prepared,protein concentration were detected by BCA protein assay method,enzyme activity of ALT,AST,lactate dehydrogenase（LDH）,r-glutamgl thanspeplidase（GGT） were detected by enzyme kinetics method and leakage were calculated.Result:The water extract of Farfarae Flos 40 g·kg-1 dose group of female had significant histopathology changes and organ coefficient of liver increased compared with control group.After co-culture for 24 hours with water extracts of Farfarae Flos,ALT leakage were significantly increased in two groups;LDH and ALT leakage were significantly increased with final concentration 0.5 g·L-1,GGT leakage was significantly increased and protein content was obviously decreased with final concentration 2.0 g·L-1 after co-culture for 6 hours with the total alkaloid of Farfarae Flos.Conclusion:Farfarae Flos displayed liver toxicity in some degree for in vivo and in vitro experiments.关键词：Farfarae Flos;total alkaloid;hepatotoxicity;precision-cut liver slice
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- 533
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发布时间：2024-09-23 - 摘要：Objective:To observe the hemodynamic influence of vertebro-basilar artery and improvement of symptoms by the treatment of（cervical spondylotic arteriopathy）CSA with heat-sensitive moxibustion therapy and Banxia Baizhu Tianma decoction.Method:The patients were randomized into a 40-case control group treated with Flunarizine Capsule and a 40-case treatment group with heat-sensitive moxibustion therapy and Banxia Baizhu Tianma decoction.Individually transcranial doppler（TCD） check was done and symptomatic change was noted.Result:Heat-sensitive moxibustion therapy and Chinese herbal medicine significantly improve the systolic peak of vertebro-basilar artery（P<0.05）,which differ significantly from the control group（P<0.05）;in treatment group the total effective rate of symptomatic improvement was 92.5%,while in control group was 77.5%.The significant difference existed（P<0.05）.Conclusion:Heat-sensitive moxibustion therapy and Banxia Baizhu Tianma decoction can significantly improve the systolic peak of vertebro-basilar artery and the therapeutic effect of CSA.关键词：cervical spondylotic arteriopathy（CSA）;heat-sensitive moxibustion therapy;Banxia Baizhu Tianma decoction;blood flow peak rate
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- 252
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发布时间：2024-09-23 - 摘要：Objective:To study the hepato-protective activity of glycyrrhizin,liquiritin and isoliquritigenin on a new HL-7702 cells injury model induced by acetaminophen.Method:HL-7702 cells injury induced by 20 mmol·L-1 acetaminophen for 3 hours were chosen as the liver cells injury model and the effect of glycyrrhizin,liquiritin and isoliquritigenin was assayed.The main index was MTT.Result:The new HL-7702 cells injury model induced by acetaminophen was successful.Glycyrrhizin,liquiritin and isoliquiritigenin had hepato-protective activity on the cell model.The minimum effective concentration of glycyrrhizin was 0.001 6 μmol·L-1.The hepato-protective maximum efficacy of glycyrrhizin was 47.0% at the concentration of 0.2 μmol·L-1.The minimum effective concentration of liquiritin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of liquiritin was 22.9 % at the concentration of 2 μmol·L-1.The minimum effective concentration of isoliquritigenin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of isoliquritigenin was 72.6%at the concentration of 250 μmol·L-1.Conclusion:The new HL-7702 cells injury model induced by acetaminophen could be applied on studying the hepato-protective effect of drugs.The hepato-protective activity of licorice has connection with not only glycyrrhizin,liquiritin but also isoliquritigenin.Therefore,it is not enough to treat glycyrrhizin,liquiritin as the quality evaluation index for licorice.关键词：Glycyrrhizin;liquiritin;isoliquritigenin;liver cell injury;hepato-protective activity
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- 1024
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发布时间：2024-09-23 - 摘要：Objective:To explore clinical efficacy of the combination of traditional Chinese medicine（TCM） and western medicine treatment of chronic pulmonary heart disease,and to evaluate its clinical value.Method:One hundred and thirty patients with chronic pulmonary heart disease in the hospital were randomized divided into the control group and the treatment group,65 cases in each group.Conventional western medicine treatment was taken in the control group.The treatment group was given TCM medicine based on the treatment of the control group,after treatment,the two groups’ patient’s indicators of lung function and clinical efficacy were analyzed and compared.Result:Indicators of lung function,such as forced expiratory volume/forced vital capacity（FEV1/FVC）,Peak expiratory flow（PEF）,maximum ventilatory volume（MVV） in the treatment group and control group were improved significantly,and indicators of lung function of patients in treatment group were better than that in the control group after treatment,the differences were statistically significant（P<0.05）.Efficiency of treatment of patients in treatment group was 93.85%,and was significantly higher than 78.46% in the control group,the difference was statistically significant（P<0.05）.Conclusion:The combination of TCM with Western medicine on pulmonary heart disease can significantly improve curative clinical effect,it has important implications in improving the quality of life and the prognosis of patients.关键词：chronic pulmonary heart disease;combination of TCM and western medicine;curative effect
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- 195
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发布时间：2024-09-23 - 摘要：目的:观察中西医结合治疗乙肝肝硬化顽固性腹水的临床疗效。方法:采用中西医结合治疗,西医治疗护肝药选注射用复方甘草酸苷针5支加入5%葡萄糖100 mL静注;降低门脉高压用心得安10 mg,2次/d,口服;改善微循环用血塞通注射液40 mL加入5%葡萄糖250 mL静滴;根据腹水情况,分别选用螺内酯40 mg,氢氯噻嗪50 mg,3次/d,口服,呋噻米20～40 mg加入5%葡萄糖60 mL静注,注意补钾,酌情补充白蛋白;合并腹膜炎者予头孢噻肟钠2 g加入0.9%生理盐水100 mL静滴;合并消化道出血予奥曲肽0.1 mg静注。中医治疗以益气疏肝、化瘀利水。方药:黄芪、茯苓、白茅根各30 g,泽兰、泽泻、虎杖、白术、贯众各15 g,白芍、炮穿山甲（先煎0.5 h）、桃仁、广木香、青皮、山楂、菟丝子各10 g,甘草6 g。随证加减:阴虚火旺者加石斛、沙参;肾气不足者加山茱萸、金樱子;便血、呕血者加白及、大黄。水煎服,1剂/d,水煎后分早、中、晚3次服。3个月后统计疗效。结果:显效14例（33.3%）,有效22例（52.4%）,无效6例（14.3%）,总有效率达85.7%。治疗结束后6个月复发病例5例,复发率为11.9%。结论:中西医结合治疗乙肝肝硬化顽固性腹水,充分发挥中西医二者的优势,提高了疗效,减少了并发症及病死率,值得推广应用。关键词：乙肝肝硬化;顽固性腹水;中西医结合
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发布时间：2024-09-23 - 摘要：目的:观察中医药辨证配合思密达外用治疗复发性口腔溃疡（RAU）的临床疗效。方法:对照组85例采用思密达外用治疗,治疗组86例在外用思密达的基础上加用中医药辨证口服药物,比较观察5 d疗效、90 d复发情况及总有效率。结果:治疗组均有效,90 d后复发仅3例,总有效率96.6%;对照组无效19例,90 d后复发10例,总有效率88.6%。结论:中医药辨证配合思密达外用治疗RAU疗效肯定,复发率低。关键词：复发性口腔溃疡;思密达;中医药辨证
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the effects of angiotensin Ⅱ receptor antagonist valsartan on large artery elasticity in patients with essential hypertension.Method:Eighty two cases of patients with essential hypertension were randomly divided into observation group（42 cases） and control group（40 cases）,the observation group was given valsartan（80-160 mg·d-1） treatment,the control group was given hydrochlorothiazide（50-100 mg·d-1） treatment,to plus metoprolol（25 mg·d-1） if blood pressure control did not meet standards（≤140/90 mmHg）in both groups,blood pressure,arm-ankle pulse wave velocity（ba-PWV）and ankle-brachial index（ABI） were applied by automatic measurement system before treatment and 6 months after treatment.Result:Before treatment,systolic blood pressure,diastolic blood pressure,ba-PWV,ABI in both groups showed no significant difference.It was comparable between the two groups.After 6 months treatment,systolic blood pressure,diastolic blood pressure in both groups of patients were decreased significantly than before treatment（P<0.05）,with no significant difference between the two groups.The ba-PWV in the observed group was significantly lower than that before treatment and the control group（P<0.01,P<0.05）.ABI in the observed group was significantly higher than that before treatment and the control group（P<0.01）.ba-PWV,ABI in the control group was no significant change than that before treatment.Conclusion:Valsartan can significantly improve arterial elasticity compared with hydrochlorothiazide at the same level on the basis of lower blood pressure,and this improvement might be also associated with factors other than treatment of hypertension.关键词：valsartan;essential hypertension;artery elasticity;arm-ankle pulse wave velocity;ankle-brachial index
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发布时间：2024-09-23 - 摘要：To investigate the application and therapeutic effects of western medicine traditional Chinese medicine（TCM）,traditional Chinese medicine,Western and TCM medicine combined with acupuncture or acupuncture point injection treated in diabetes mellitus（DM） and its complications（gastro paresis,neurogenic bladder,peripheral neuropath,mood disorders,hypertension,retinopathy,peripheral neuropathy,coincide with shingles,insulin resistance,diabetic foot gangrene） in clinical.Studies have show that drugs combined with acupuncture has a clear advantage in efficacy compared with simply acupuncture or drugs applied in DM and its complications.But most research of acupuncture combined with drugs in the treatment of diabetes and its complications belong to small sample observed in clinical and mechanism is not yet clear.So to explore drugs and acupuncture interaction in therapy,develop safe and effective new agent,which is to provide a reference for the treatment of DM.关键词：acupuncture combined with medicine;diabetes;complication;preparation
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- 890
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发布时间：2024-09-23 - 摘要：To analyze the reports on the relationships between tetramethylpyrazine（TMPZ） and the intracellular free calcium concentration under platelet activation,and to provide hint for further study of the pharmacological actions of TMPZ.By retrieving the following databases such CNKI,Wanfang,PubMed,and so on,to read relative publications.The current studies show that the intracellular calcium concentration in platelets increases due to platelet activation,which depends on the entry of calcium in the extracellular matrix and the release of calcium in endoplasmic reticulum.TMPZ plays its inhibitory role on platelet activation mainly through the suppression on the increase of the intracellular calcium concentration.It still remains unknown about the mechanism underlying the inhibition on platelet activation by TMPZ,and most of the studies suggest that TMPZ inhibits the intracellular free calcium concentration,leading to the suppression of platelet activation.However,those studies are much descriptive,instead of time-lapse observation on it.关键词：tetramethylpyrazine;platelet receptor;platelet activation;calcium signaling
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- 302
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发布时间：2024-09-23 - 摘要：To analyze the correlations between hepatotoxicity and four properties,five tastes,meridian entry of Chinese materia medica.Chinese herbs with hepatotoxicity were regarded as the research objects,which were collected from published reports and monographs.The property theory of Chinese materia medica were referred to<Dictionary of Chinese Materia Medica>.The regularities of four properties,five tastes,and meridian entry of Chinese herbs with hepatotoxicity,and the correlations between hepatotoxicity and property theory of Chinese materia medica were analyzed.① The differences in terms of four properties of Chinese herbs with hepatotoxicity or general Chinese herbs were obvious（P<0.05）,and the order from high to low was cold,warm,neutral,cool and hot,which was similar between two kinds of Chinese herbs.There was no correlation between hepatotoxicity and four properties;②The difference in terms of five tastes of Chinese herbs with hepatotoxicity was obvious（P<0.05）,and the order from high to low was bitter,acrid,sweet,sour,astringent,salty and bland.The difference in terms of five tastes of of general Chinese herbs was also obvious（P<0.05）,and the order from high to low was bitter,sweet,acrid,salty,astringent,sour and bland.There existed some correlation between hepatotoxicity and five tastes（P<0.05,rp=0.137）;③The differences in terms of meridian entry of Chinese herbs with hepatotoxicity or general Chinese herbs were obvious（P<0.05）,and the order from high to low was liver,lung,stomach,spleen,kidney,heart,large intestine,bladder,gallbladder,small intestine,pericardium and triple energizer meridian,which was similar between two kinds of Chinese herbs.There was no correlation between hepatotoxicity and meridian entry.The differences in terms of four properties,five tastes and meridian entry of Chinese herbs with hepatotoxicity are obvious.Comparied with general Chinese herbs,there is no correlation between hepatotoxicity and four properties or meridian entry,while there is some weak correlation between hepatotoxicity and five tastes.关键词：Chinese herbs;hepatotoxicity;four properties;five tastes;meridian entry
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发布时间：2024-09-23 - 摘要：The morphological character,pharmacological effect and reproduction of Ornithogalum caudatum were reviewed.Recent books and journals for morphological character,pharmacological effect and reproduction of O.caudatum were summarized.The botanical morphology,biological characteristics and harvesting conditions was introduced.Pharmacological references showed that O.caudatum had the effects on enhancing immunity,anti-hepatoma,anti-liver damnification and anti-inflammation.The classical and tissue culture reproduction methods of O.caudatum were summarized.In recent years,relatively less research has been carried out on O.caudatum.So some suggestions for pharmacological effect and reproduction of O.caudatum were put forward in this paper.关键词：Ornithogalum caudatum;morphological character;pharmacological effect;reproduction
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- 309
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发布时间：2024-09-23 - 摘要：阐述《金匮要略.血痹虚劳病脉证并治第六》"虚劳诸不足,风气百疾,薯蓣丸主之"的记载,从理论上来分析薯蓣丸治疗艾滋病的可行性与合理性。通过对对"风气百疾"的认识,分析艾滋病和虚劳病病因病机的相似性,虚劳病的治疗原则是滋脾祛邪,即循滋脾之径,收益气之功,体现出艾滋病扶正重视滋脾的主导思想。分析艾滋病最常见的类型,可以将艾滋病的治疗、放在最具有代表性的肺脾气虚证患者来研究治疗方法。阐述艾滋病肺脾气虚证患者与《金匮要略》中虚劳病的关系,为中医药防治艾滋病提供更为广阔的思路和方法。关键词：风气百疾;虚劳病;艾滋病
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- 421
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发布时间：2024-09-23 - 摘要：Objective:To predict the potential molecular target proteins of multiple constituents of Shenmai San absorbed in plasma based on computational systems biology approaches.Method:TCMGeneDIT was used to collected data regarding genes or proteins affected by Panax ginseng,Ophiopogon japonicus.and Schisandra chinensis.Based on the chemical structure of 14 main compounds absorbed into plasma after oral administration of Shenmai San,target identification and analysis were conducted to determine the target proteins of those compounds using PharmMapper sever.The proteins which have direct interaction with predictive target proteins were selected by screening BIND,ioGRID,DIP,HPRD,IntAct,MINT database.Result:Fifty five genes or proteins associated with P.ginseng were found.One gene or protein associated with S.chinensis was found.Four components of P.ginseng not only directly interact with target proteins which were proved by experiments,but also interact with other related proteins.Ten components of S.chinensis can only play assistant roles by interacting with related proteins.Conclusion:The result provided useful evidence for further research.It is expected that it be helpful for understanding the molecular mechanism of Shenmai San.Taken together,the protocol developed in this study may lead to a deeper and systematc understanding in the mechanism of Chinese formula.关键词：computational systems biology;Shenmai San;molecular target protein
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- 441
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发布时间：2024-09-23 - 摘要：Objective:To seek use medicine mechanism of treat Hand-foot-mouth disease.Method:Use gray relational analysis analyse a database of Chinese Herbal Compound treat the hand-foot-mouth disease.Result:According to evaluate correlation degree of the gray correlation coefficient,confirmation of Gardenia,Scutellaria,Herba Lophatheri,Talc,Patchouli,Coix seed as a treatment with commonly used drugs,but the gray correlation coefficients priorities and use frequency is not entirely positive correlation.Conclusion:Hand-foot-mouth disease syndrome as an example,heat syndrome,followed by the deficiency and cold syndrome,disease in lung,heart,spleen,in the diagnosis of drugs mainly to taste of bitter and sweet cold Qingre Jiedu Qushi and diaphoretic.关键词：hand-foot-mouth disease;traditional Chinese medicine;gray relational analysis
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- 137
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发布时间：2024-09-23 - 摘要：征询业内多位权威专家,考稽古今多部著作,确定治疗半表半里证的方剂共计14首,涉及中药43味。运用数理统计学的方法,通过古今剂量折算,对药物使用频次,药物"四气、五味"属性,药物之间的配对,药物剂量等进行分析,探讨其配伍,力求阐明半表半里证的用药规律。此类方主要重用黄芩、柴胡、半夏、槟榔、厚朴、草果等具有和解、清热、燥湿之功之药物,佐以芳香、清热、理气、化湿,注重辛开苦降气味配伍,使枢机利、三焦畅、膜原达,从而达到邪有出路,病症除之目的。关键词：半表半里证;方剂;中药;中药配伍;文献
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中
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