最新刊期
卷 16 ， 期 5 ， 2010
- 摘要：Aim：To study the protective therapeutic action and mechanism of Liuwei Dihuang Decoction、Buzhong Yiqi Decoction and Compound Danshen Decoction on the marrow- suppressed mice. Methods：the expression of the TPO、c-Mpl and GATA-1 mRNA was detected by RT-PCR technique simultaneously. Result：Liuwei Dihuang Decoction and Buzhong Yiqi Decoction were shown to promote the expression of TPO、c-Mpl and GATA-1 mRNA in spleen of mice，through which they can accelerate the marrow cells of marrow-suppressed mice. Compound Danshen Decoction was not shown to promote the expression of Thrombopoietin（TPO）mRNA、Thrombopoietin receptor（c-Mpl）mRNA and GATA-1 mRNA in spleen of mice, but slso can accelerate Peripheral blood cells of marrow-suppressed mice. Conclusion：Peripheral blood cells of marrow-suppressed mice were increased significantly by Liuwei Dihuang Decoction、Buzhong Yiqi Decoction and Compound Danshen Decoction.关键词：[Key words] Liuwei Dihuang Decoction;Buzhong Yiqi Decoction;Compound Danshen Decotion;marrow suppress;TPO mRNA;c-Mpl mRNA;GATA-1 mRNA
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发布时间：2024-09-23 - 摘要：Objective:To set up a proper technology of refining of the aqueous extract of Wuzhuyu-tang. Method:7 kind components which had been improved to be absorbable pharmacological ingredients were chosen as index for refining. The static adsorption,dynamic adsorption and leakage curve experiments were done to choose a proper resin. By means of single-factor experiment and orthogonal experiment,the optimum refining conditions were found,such as adsorption time,proportion of sample weight and resin volume,proportion of diameter and height, concentration of decoction and eluent,flow rate,eluent volume and so on. Result: The AB-8 resin had the best separating efficiency when the content in the aqueous extract was 0. 33g per 1mL equivalent to raw material,the ratio of dia-height was 1: 4,and the volume of 60% ethanol as eluant 8BV with desorption-power 0. 1BV /min. Conclusion: The content of 7 kind components were over 70% after refining,5 of them were over 90% . It is concluded that the optimized extracting and refining technology is reasonable and feasible.关键词：Wuzhuyu-tang;macroporous resin;refining technology;effective substances
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发布时间：2024-09-23 - 摘要：Objective: To study the effects of extractives from Pericarpium citri reticulatae(PCR) and Pogostemon cablin(Blanco)Benth(PCB)on limb Ischem ia/Reperfusion(LIR)model rat’s gastro- intestinal motility. Methods: Adopt LIR model,observe the effects of PCR water extractives,Hesperidin, PCB water extractives,PCB water extractives plus volatile oil and Patchouli alcohol on LIR model rats’ gastric emptying function and small intestinal propulsive function. Results: Contrast to the normal control group ,The LIR model rats’ gastric emptying function and small intestinal propulsive function degenerated significantly(P<0.01). PCR water extractives，Hesperidin， PCB water extractives and PCB water extractives plus volatile oil could significantly mprove the LIR model rats’ gastric emptying function and small intestinal propulsive function(P<0.01),but Patchouli alcohol had no effect.There was significant difference between PCR water extractives group and Hesperidin group, between PCB water extractives group and PCB water extractives plus volatile oil group(P<0.01). Conclusion: In the aspect of improving the LIR model rats’ gastric emptying function and small intestinal propulsive function, PCR water extractives had better effect than Hesperidin,and PCB water extractives plus volatile oil had better effect than PCB water extractives, but Patchouli alcohol had no effect.关键词：limb Ischem ia/Reperfusion LIR gastric emptying function small intestinal propulsive function
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发布时间：2024-09-23 - 摘要：Technological conditions of carbonizing process of Nodus Nelumbinis Rhizomatis were selected by orthogonal test according to the contents of total polyphenol and water soluble extract combined with the characteristics of finished products. Pilot experiments conditions of the carbonizing process of Nodus Nelumbinis Rhizomatis were studied by using CG-Y7502 intellectualized herbal medicine roaster manufactured by Chun Jiang Institute of Automation. Result: Temperature for charring Nodus Nelumbinis Rhizomatis was 200 ℃ for 22 minutes in laboratory when the weight of Nodus Nelumbinis Rhizomatis is 300 g. The weight of Nodus Nelumbinis Rhizomatis was 10 kg for 10 minutes at the speed of 26r /min,temperature ranging from 195 ℃ to 205 ℃ .关键词：Nodus Nelumbinis Rhizomatis;carbonizing process;total polyphenol;water extract
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发布时间：2024-09-23 - 摘要：ABSTRACT: Objective: To explore protective effect and mechanism of breviscapine against myocardial ischemia reperfusion injury in rats. Methods: 40 SD rats were divided into sham operation group , ischemia reperfusion group and breviscapine group randomly.Rats in breviscapine group were injected with breviscapine 25、50mg.kg1.d-1 respectively prior to ischemia reperfusion .other two groups were given NS instead of breviscapine equally at a week before the operation. Myocardial ischemia reperfusion injury model was established. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with AnnexinⅤ-EGFPand propidium iodiode ( PI) staining. The ultrastructural alterations were observed under the transmission electron microscopy. Results : breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia reperfusion injury obviously（P＜0.05，P＜0.01）;The degree of injuring on ultrastructures of myocardial cell in breviscapine group is rather alleviated than in ischemia reperfusion group.Conclusion : breviscapine may protect myocardial cells during ischemia reperfusion injury and the protective mechanism may be obtained by inhibiting myocardial apoptosis.关键词：breviscapine;apoptosis;Cardiomyocyte;ischemia reperfusion;myocardiac ultrastructures
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发布时间：2024-09-23 - 摘要：Objective:To prepare compound Danshen pellets. Method: The compound Danshen pellets were prepared by using a centrifugal granulator. The composition and process factors were optimized by adopting several indices such as sphericity of pellets,repose angle,bulk density and friability. Result: The optimal process parameters were as follows:the quality ratio of Borneol-β-Cyclodextrinclusion Complexes and MCC was 4∶ 3,binder solvent was 40% syrup,which including extractive. The rotating rate of plate was 200 r·min -1,the blower rate was 8L·min -1,the rate of air flow was 18 L·min -1,the spray air pressure was 0. 06 MPa,the rotating rate of spray solution pump was 12 ～ 17 r·min -1 and the rotating rate of powder feed machine was 15 ～ 20 r·min -1. The active components could release more than 85% in 15 minutes. Conclusion: Under the optimal conditions,the compound Danshen pellets prepared by using the centrifugal granulator have prefect shape and surface characteristics,the yield of target size pellets are more than 85% . The three index components could release fast and synchronically.关键词：compound Danshen;pellets;centrifugal granulator
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发布时间：2024-09-23 - 摘要：Objective: To study polar constituents of Mosla chinensis 'jiangxiangru’. Method: Silica gel column chromatography, Sephadex LH-20 gel column chromatography and MPLC were employed for the isolation and purification.The structures were identified on the basis of spectral data and chemical methods. Result: Six polar compounds were identified as follows:methyl-3-(3',4'-dihydroxyphenyl)lactate(1)、corchoionoside C(2)、prunasin(3)、sambunigrin(4)、benzyl-D-glucopyranoside(5)and (s)-pencedanol-7-O-β-D-glucopyranoside(6). Conclusion: These compounds were isolated for the first time from Mosla chinensis 'jiangxiangru’and have not been previously described in genus Mosla.关键词：Mosla chinensis 'jiangxiangru';genus Mosla;polar constituents
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发布时间：2024-09-23 - 摘要：目的:比较白附子微米药材与其原药材中所含成分,探讨微米药材在祛除白附子刺激性的同时,固有物质保留程度。方法:采用高效液相色谱及薄层色谱实验比较差异。结果:白附子微米药材与原药材成分色谱峰基本一致,传统炮制饮片水提取物成分峰较微米药材与原药材明显减少。结论:白附子经超微粉碎后,基本保存原成分。药理实验结果证明,白附子微米药材在明显减轻了白附子毒性、刺激性的同时,原成分基本保存。关键词：白附子微米药材;薄层;高效液相色谱
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发布时间：2024-09-23 - 摘要：Objective: To set up a proper technology of refining of the aqueous extract of Wuzhuyu-tang. Method: 7 kind components which had been improved to be absorbable pharmacological ingredients were chosen as index for refining. The static adsorption,dynamic adsorption and leakage curve experiments were done to choose a proper resin. By means of single-factor experiment and orthogonal experiment,the optimum refining conditions were found,such as adsorption time, proportion of sample weight and resin volume,proportion of diameter and height,concentration of decoction and eluent,flow rate,eluent volume and so on. Result: The AB-8 resin had the best separating efficiency when the content in the aqueous extract was 0.33g per 1mL equivalent to raw material,the ratio of dia-height was 1:4,and the volume of 60% ethanol as eluant 8BV with desorption-power 0.1BV/min. Conclusion: The content of 7 kind components were over 70% after refining, 5 of them were over 90%. It is concluded that the optimized extracting and refining technology is reasonable and feasible.关键词：Wuzhuyu-tang;macroporous resin;refining technology;effective substances
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发布时间：2024-09-23 - 摘要：Objective:To investigate the method for using semi-bionic （SBE） on Kangxuanyun granules as an example. Method: Orthogonal design was used to optimize the extraction conditions of SBE with the evaluation markers such as alisol B-acetate,tetramethylpyrazine,ferulic acid,total alkaloids,the total contents of emodin and chrysophanol,dried extract. Result: The optimized SBE conditions were as follows: The pH of the three extractions were in proper order 2. 00,7. 50,8. 50; the solvent volume was 10,8,6 times; and the extraction time was 2. 0, 1. 5,1. 5 h,respectively. Conclusion: The optimum extraction conditions are stable and practicable,and surpass the original extraction conditions.关键词：Kangxuanyun granules;semi-bionic extraction;orthogonal design;HPLC;UV;general evaluation
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发布时间：2024-09-23 - 摘要：Technological conditions of carbonizing process of Nodus Nelumbinis Rhizomatis were selected by orthogonal test according to the contents of total polyphenol and water soluble extract combined with the characteristics of finished products. Pilot experiments conditions of the carbonizing process of Nodus Nelumbinis Rhizomatis were studied by using CG-Y7502 intellectualized herbal medicine roaster manufactured by Chun Jiang Institute of Automation. Result: Temperature for charring Nodus Nelumbinis Rhizomatis was 200 ℃ for 22 minutes in laboratory when the weight of Nodus Nelumbinis Rhizomatis is 300 g. The weight of Nodus Nelumbinis Rhizomatis was 10 kg for 10 minutes at the speed of 26r/min, temperature ranging from 195 ℃ to 205 ℃.关键词：Nodus Nelumbinis Rhizomatis;carbonizing process;total polyphenol;water extract
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发布时间：2024-09-23 - 摘要：目的:研究仙灵骨葆滴丸的最佳成型工艺。方法:以硬度、圆整度、溶散时限、得率作为评价指标,对药物与基质的比例、滴制温度、冷却剂上部温度3因素进行正交试验。结果:优选的最佳成型工艺是二甲硅油350cs作为冷却液,处方配比为:提取物∶聚乙二醇1500（PEG1500）∶聚乙二醇4000（PEG4000）（1∶0.8∶0.4）,滴制液温度85±2℃,冷却剂上部温度为35±2℃,滴丸成型率最高。结论:以筛选出的成型工艺条件制成的滴丸,成品得率高。关键词：仙灵骨葆滴丸;正交试验;制剂工艺
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发布时间：2024-09-23 - 摘要：Objective: To prepare compound Danshen pellets. Method : The compound Danshen pellets were prepared by using a centrifugal granulator. The composition and process factors were optimized by adopting several indices such as sphericity of pellets, repose angle, bulk density and friability. Result : The optimal process parameters were as follows:the quality ratio of Borneol-β-Cyclodextrinclusion Complexes and MCC was 4 ∶3,binder solvent was 40% syrup, which including extractive. The rotating rate of plate was 200 r ·min-1, the blower rate was 8L ·min-1, the rate of air flow was 18 L ·min-1, the spray air pressure was 0.06 MPa, the rotating rate of spray solution pump was 12~17 r ·min-1 and the rotating rate of powder feed machine was 15~20 r ·min-1. The active components could release more than 85% in 15 minutes. Conclusion : Under the optimal conditions,the compound Danshen pellets prepared by using the centrifugal granulator have prefect shape and surface characteristics, the yield of target size pellets are more than 85%. The three index components could release fast and synchronically.关键词：compound Danshen;pellets;centrifugal granulator
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发布时间：2024-09-23 - 摘要：目的:以地黄中水苏糖含量和出膏率为指标优选地黄寡糖有效部位的提取工艺。方法:以水苏糖为指标成分,采用正交实验法对地黄寡糖有效部位的提取工艺进行优选。结果:优选工艺为采用水煎煮提取,加10倍量的水,提取3次,每次1h。结论:优选出的工艺科学合理。关键词：水苏糖;出膏率;提取工艺;正交实验
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》是经中国科技部批准,由国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的学术刊物。已成为"中国科技论文统计源期刊"（中国科技核心期刊）;"中国中文核心期刊"、"中国学术期刊综合评价数据库"来源期刊;"中国期刊网、中国学术期刊(光盘
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发布时间：2024-09-23 - 摘要：Objective: To analysis Peaonia lactiflora Pall. flower petal volatile compounds after eaten by Mylabris speoiosa Pallas. Method: By steam distillation to extract dry petals volatile compounds,petroleum ether soak extract fresh petals volatile compounds,GC-MS analysis,identify the chemical components. Result: The eaten dry flowers volatile compounds rate is obviously higher. It is 0. 13% and 0. 06% （mL·g -1）,but it is not change the relative content. Dry and fresh flower were identified about 34 and 19 species of chemical composition. Fresh flower contains more small molecular component,such as formaldehyde,benzene,phenylethyl acetaldehyde,etc. Conclusion: The eaten flower petals total volatile compounds and the relative content of each component has affected. The main components of fresh and dry flower petals are different.关键词：Flower petal of Peaonia lactiflora Pall.;Mylabris speoiosa Pallas;Eat;volatile compounds;GC-MS
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发布时间：2024-09-23 - 摘要：Objective: To establish a fast method to evaluate the geographic origins of salviae miltiorrhizae radix et rhizoma and the contents of salvianolic acid B based on infrared spectroscopy （IR）. Method: IR spectra of forty samples collected from 4 different origins were analyzed to search for the characteristic peaks on second derivative （2D） spectra to reflect the characteristics of origin,also to search for a characteristic peak on normalized spectra for fast quantitation of salvianolic acid B. Result: Some characteristic peaks on 2D spectra were found which reflect 4 different origins. The intensity of 1 262 cm -1 on normalized spectra was found to correlate well with the content of salvianolic acid B determined by high performance liquid chromatography, which realize the fast quantitative analysis of salvianolic acid B. Conclusion: The proposed IR method provides a new alternative of fast quality evaluation of salviae miltiorrhizae radix et rhizoma for manufacture of traditional Chinese medicine.关键词：Salvia miltiorrhiza Bge.;infrared spectroscopy;salvianolic acid B
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发布时间：2024-09-23 - 摘要：Objective: To analysis Peaonia lactiflora Pall. flower petal volatile compounds after eaten by Mylabris speoiosa Pallas. Method: By steam distillation to extract dry petals volatile compounds, petroleum ether soak extract fresh petals volatile compounds, GC-MS analysis, identify the chemical components. Result: The eaten dry flowers volatile compounds rate is obviously higher. It is 0.13% and 0.06% (mL·g-1), but it is not change the relative content. Dry and fresh flower were identified about 34 and 19 species of chemical composition. Fresh flower contains more small molecular component, such as formaldehyde, benzene, phenylethyl acetaldehyde, etc. Conclusion: The eaten flower petals total volatile compounds and the relative content of each component has affected. The main components of fresh and dry flower petals are different.关键词：Flower petal of Peaonia lactiflora Pall.;Mylabris speoiosa Pallas;Eat;volatile compounds;GC-MS
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发布时间：2024-09-23 - 摘要：Objective:To establish an accurate method for the determination of astragaloside Ⅳ in Paichuan Tablets. Method:Diamonsil C18（4. 6 mm × 250 mm,5 μm）,with a mobile phase consisting of acetonitrile-H2O（32∶ 68）,the detector is Evaporative Light-scattering Detector,The drift tube temperture of 50 ℃ ,the flow rate was 1. 0 mL·min-1. Result:A good linearity of astragaloside Ⅳ was obtained within the range of 0. 41 ～ 2. 45 μg,the average recovery was 100. 04% and RSD was 0. 14% . Conclusion:The method was simple and accurate,and can be used for the quality control of Paichuan Tablets.关键词：Paichuan Tablets;astragaloside Ⅳ;HPLC;determination
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发布时间：2024-09-23 - 摘要：Objective: To establish a fast method to evaluate the geographic origins of salviae miltiorrhizae radix et rhizoma and the contents of salvianolic acid B based on infrared spectroscopy (IR). Method: IR spectra of forty samples collected from 4 different origins were analyzed to search for the characteristic peaks on second derivative (2D) spectra to reflect the characteristics of origin, also to search for a characteristic peak on normalized spectra for fast quantitation of salvianolic acid B. Result: Some characteristic peaks on 2D spectra were found which reflect 4 different origins. The intensity of 1 262 cm-1 on normalized spectra was found to correlate well with the content of salvianolic acid B determined by high performance liquid chromatography, which realize the fast quantitative analysis of salvianolic acid B. Conclusion: The proposed IR method provides a new alternative of fast quality evaluation of salviae miltiorrhizae radix et rhizoma for manufacture of traditional Chinese medicine.关键词：Salvia miltiorrhiza Bge.;infrared spectroscopy;salvianolic acid B
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发布时间：2024-09-23 - 摘要：Objective: To establish an accurate method for the determination of astragaloside Ⅳ in Paichuan Tablets. Method: Diamonsil C18(4.6 mm×250 mm,5 μm),with a mobile phase consisting of acetonitrile-H2O(32 ∶68),the detector is Evaporative Light-scattering Detector,The drift tube temperture of 50 ℃,the flow rate was 1.0 mL ·min-1. Result :A good linearity of astragaloside Ⅳ was obtained within the range of 0.41~2.45 μg, the average recovery was 100.04% and RSD was 0.14%. Conclusion :The method was simple and accurate, and can be used for the quality control of Paichuan Tablets.关键词：Paichuan Tablets;astragaloside Ⅳ;HPLC;determination
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发布时间：2024-09-23 - 摘要：目的:对6种较典型的后下中药饮片的主要有效成分煎出量及随煎药时间的变化规律进行研究。方法:采用毛细管GC和HPLC色谱法。结果:薄荷脑、广藿香油、樟脑、钩藤碱、橙花叔醇和肉豆蔻醚分别在0.0039～0.039μg、0.5000～2.5000μL、0.2100～1.2500μg、0.1900～0.9500μg、0.0063～2.0000μg和0.4000～2.0000μg范围内与各色谱峰面积呈线性关系。结论:易挥发芳香类后煎药的煎药时间控制在3min内比较合适,以生物碱为主要成分的后煎药,煎药时间控制在10～15min比较合适。关键词：中药饮片;气相色谱法;高效液相色谱法
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发布时间：2024-09-23 - 摘要：Objective:To develop an HPLC method for the simultaneous determination of ferulic acid and icariin in the Li’ankang Tablets by HPLC. Method: The separation was preformed on a Phenomenex Luna C18 column with acetonitrile-0. 1 % acetic acid on 316 nm at 1. 0 mL·min -1. Result: The good linear correlation of ferulic acid was observed from 0. 052 8 μg to 0. 184 8 μg（r = 0. 999 9） and that of icariin was observed from 5. 68 μg to 19. 88 μg （r = 0. 999 9）. The average recoveries of ferulic acid and icariin were 99. 8% to 101. 1% and 99. 9% to 101. 5% respectively. Conclusion: The established method can be used as a quality controlling method for Li’ankang Tablet.关键词：Li’ankang Tablet;ferulic acid;icariin;HPLC
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发布时间：2024-09-23 - 摘要：目的:对蒡芩慢咽滴丸中牛蒡苷的含量及溶出度进行了测定,以考察其内在的质量。方法:采用十八烷基键合硅胶色谱柱（4.6mm×250mm,5μm）,以甲醇-0.2%磷酸溶液（45∶55）为流动相,流速为1.0mL·min-1,检测波长为280nm。并应用浆法,对蒡芩慢咽滴丸中牛蒡苷进行了溶出度测定。结果:牛蒡苷的线性范围为0.48～2.40μg（r=0.9996）,平均回收率（n=6）为99.97%（RSD=0.47%）;并以含0.5%十二烷基硫酸钠的蒸馏水为溶剂,转速100r·min-1,经45min,溶出度为标示量的75%以上。结论:该方法简单、迅速、可靠;是控制中药复方内在质量的一种可行方法。关键词：蒡芩慢咽滴丸;牛蒡苷;溶出度;高效液相色谱法
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发布时间：2024-09-23 - 摘要：Objective: To develop an HPLC method for the simultaneous determination of ferulic acid and icariin in the Liankang Tablets by HPLC. Method : The separation was preformed on a Phenomenex Luna C18 column with acetonitrile-0.1 % acetic acid on 316 nm at 1.0 mL ·min-1. Result : The good linear correlation of ferulic acid was observed from 0.052 8 μg to 0.184 8 μg( r =0.999 9) and that of icariin was observed from 5.68 μg to 19.88 μg ( r =0.999 9). The average recoveries of ferulic acid and icariin were 99.8% to 101.1% and 99.9% to 101.5% respectively. Conclusion: The established method can be used as a quality controlling method for Liankang Tablet.关键词：Liankang Tablet;ferulic acid;icariin;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To establish the determination method for oleanolic acid in dandelion by HPLC. Method: HPLC was carried out on Hypersil ODS2 C18 （4. 6 mm × 250 mm,5 μm） at 30 ℃ using methanol-0. 5% H3PO4 （pH = 3. 5）（90∶ 10） as mobile phase with a flow rate of 1. 0 mL·min -1 and detection wavelength was at 210 nm. Result:The oleanolic acid was linear within the range of 0. 872 ～ 7. 848 μg. The average recovery was 98. 25% and RSD was 1. 54% . Conclusion:This method is useful for quality control of dandelion.关键词：dandelion;oleanolic acid;determination
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- 200
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发布时间：2024-09-23 - 摘要：Objective:To determine the content of conduriol A in Guangxi Gymnema sylvestre（Retz. ）Schult by TLCS. Method:Silica H thin layer plate was used; the developer reagent was benzene:ethyl acetate:methanol （10∶ 3∶ 5）,the color reagent was 5% vanillin sulfate solution. The plate was at heating on 105 ℃ ,when the spot was clear; the detection wavelength was 538 nm and the reference wavelength was at 700 nm. Result: The conduriol A was linear in range of 1. 025 ～ 6. 150 μg; the average recovery was 98. 84% ,RSD = 2. 02% （n = 6）. The average content of conduriol A was 2. 08% in was simple,sensitive,accurate and had good reproducibility. Conclusion: The method can be used the identification and determination method of Guangxi Gymnema Sylvestre（ Retz. ） Schult and qualitatively.关键词：Gymnema sylvestre（Retz. ）Schult;conduriol A;TLCS
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发布时间：2024-09-23 - 摘要：Objective: To establish the determination method for oleanolic acid in dandelion by HPLC. Method: HPLC was carried out on Hypersil ODS2 C18(4.6 mm×250 mm,5 μm)at 30 ℃ using methanol-0.5% H3PO4 (pH=3.5)(90 ∶10) as mobile phase with a flow rate of 1.0 mL ·min-1 and detection wavelength was at 210 nm. Result :The oleanolic acid was linear within the range of 0.872~7.848 μg. The average recovery was 98.25% and RSD was 1.54%. Conclusion :This method is useful for quality control of dandelion.关键词：dandelion;oleanolic acid;determination
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发布时间：2024-09-23 - 摘要：目的:采用HPLC-ELSD法测定黄芪根、茎叶中黄芪甲苷的含量。方法:HPLC-ELSD法,Luna C18柱（4.6mm×150mm,5μm）,流动相:乙腈-水（35∶65）,流速:1.0mL·min-1;ELSD参数为漂移管温度105℃,载气流速2.8L·min-1。结果:不同产地黄芪根中黄芪甲苷的含量分别为0.096%、0.116%、0.081%,黄芪茎叶中黄芪甲苷的含量分别为0.219%、0.198%、0.232%。结论:黄芪茎叶中黄芪甲苷的含量明显高于黄芪根,黄芪茎叶有潜在的开发价值。关键词：黄芪;黄芪甲苷;根;茎叶
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- 349
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- 19
发布时间：2024-09-23 - 摘要：Objective: To determine the content of conduriol A in Guangxi Gymnema sylvestre (Retz.)Schult by TLCS. Method :Silica H thin layer plate was used; the developer reagent was benzene:ethyl acetate:methanol(10 ∶3 ∶5), the color reagent was 5% vanillin sulfate solution.The plate was at heating on 105 ℃,when the spot was clear; the detection wavelength was 538 nm and the reference wavelength was at 700 nm. Result : The conduriol A was linear in range of 1.025~6.150 μg; the average recovery was 98.84%, RSD=2.02%(n=6).The average content of conduriol A was 2.08% in was simple, sensitive, accurate and had good reproducibility. Conclusion : The method can be used the identification and determination method of Guangxi Gymnema Sylvestre (Retz.)Schult and qualitatively.关键词：Gymnema sylvestre (Retz.)Schult;conduriol A;TLCS
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:建立冬凌草饮片的含量测定方法,并对市售饮片进行含量分析,以考察其质量。方法:色谱柱:Agilent ZORBAX Eclispse XDB-C18柱（4.6mm×150mm,5μm）,流动相:甲醇∶水（50∶50）,检测波长:240nm。结果:冬凌草甲素在0.41～1.02μg范围内呈良好线性关系,r=0.9998,加样回收率平均为99.2%（n=9,RSD=1.5%）。结论:该法灵敏度高、操作简便、结果准确。可用于冬凌草饮片的含量测定。关键词：冬凌草;冬凌草甲素;高效液相色谱;含量测定
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- 135
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- 3
发布时间：2024-09-23 - 摘要：Objective: To investigate and screen the active compounds of Huanglianjiedu decoction（HLJD） for senile dementia. Method: Inhibition on acetylcholinesterase and antioxidant activity by HLJD was studied with ultraviolet spectrometry. The single herbs and the major compounds of HLJD were also determined and compared by a rapid and effective acetylcholinesterase inhibition assay and antioxidant assay. Result: The formula HLJD had a better antioxidant activity than single herbs except for Radix Scutellaria which is rich in polyphenolic compounds. The most active conpounds was baicalin （ IC50 = 12. 06 μg·mL -1 ）. The results also indicate that the most active herbs and compounds for AChE inhibition were Coptidis Rhizoma （IC50 = 18. 89 μg·mL -1） and berberine （ IC50 = 9. 52 μg·mL -1）; Radix Scutellariae,Fructus Gardenia as well as the major compounds of them showed no or lower inhibitory activity. Conclusion: It can be concluded that alkaloid compounds in HLJD contribute to the inhibitory activity for acetylcholinesterase and flavanoids compounds contribute to the antioxidant activity.关键词：Huanglianjiedu decoction;DPPH;AChE;senile dementia
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- 903
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- 52
发布时间：2024-09-23 - 摘要：Objective: To extract,separate and identify the water-soluble chemical constituent from Radix Isatidis. Method: The Radix Isatidis water extract underwent adsorption by HP-20 macroporous resin,the portion eluted by ethanol of different content was isolated and purified on silica gel column repeatedly. The structures of the compounds were identified by spectra data. Result: 6 compounds temporarily separated from Radix Isatidis water extract,were identified as: （ + ）-isolariciresinol（1）,lariciresinol-4-bis-O-β-D-glucopyranoside（2）,lariciresinol-4, 4′-bis-O-β-D-glucopyranoside （ 3 ）,epigoitrin （ 4 ）,indole-3-acetonitrile-6-O-β-D-glucopyranoside （ 5 ）, 4-hydroxyindole-3-carboxaldehyde（6）. Conclusion: For the first time,Compound 6 was isolated from Radix Isatidis.关键词：Radix Isatidis;water-soluble chemical composition;alkaloids
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- 708
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- 18
发布时间：2024-09-23 - 摘要：Objective: To investigate and screen the active compounds of Huanglianjiedu decoction(HLJD) for senile dementia. Method: Inhibition on acetylcholinesterase and antioxidant activity by HLJD was studied with ultraviolet spectrometry. The single herbs and the major compounds of HLJD were also determined and compared by a rapid and effective acetylcholinesterase inhibition assay and antioxidant assay. Result: The formula HLJD had a better antioxidant activity than single herbs except for Radix Scutellaria which is rich in polyphenolic compounds. The most active conpounds was baicalin (IC50=12.06 μg ·mL-1). The results also indicate that the most active herbs and compounds for AChE inhibition were Coptidis Rhizoma (IC50=18.89 μg ·mL-1) and berberine (IC50=9.52 μg ·mL-1); Radix Scutellariae, Fructus Gardenia as well as the major compounds of them showed no or lower inhibitory activity. Conclusion: It can be concluded that alkaloid compounds in HLJD contribute to the inhibitory activity for acetylcholinesterase and flavanoids compounds contribute to the antioxidant activity.关键词：Huanglianjiedu decoction;DPPH;AChE;senile dementia
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:建立野菊花中绿原酸的HPLC分析方法,并对10个产地的市售野菊花药材进行分析,考察野菊花药材的质量。方法:采用AgilentXDE-C18色谱柱（4.6mm×150mm,5μm）,流动相为甲醇-冰醋酸-水（18∶1∶81）,流速为1.0mL·min-1,检测波长为327nm,柱温为20℃。结果:不同产地的市售野菊花药材的绿原酸含量差别很大。结论:所建立的野菊花中绿原酸的定量分析方法重复性好,专属性强,简便迅速;对10批药材的分析结果表明野菊花药材质量差别很大,有必要进一步加强质量控制。关键词：野菊花;绿原酸;含量测定
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- 321
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- 8
发布时间：2024-09-23 - 摘要：Objective: To extract,separate and identify the water-soluble chemical constituent from Radix Isatidis. Method: The Radix Isatidis water extract underwent adsorption by HP-20 macroporous resin, the portion eluted by ethanol of different content was isolated and purified on silica gel column repeatedly. The structures of the compounds were identified by spectra data. Result: 6 compounds temporarily separated from Radix Isatidis water extract,were identified as: (+)-isolariciresinol(1) ,lariciresinol-4-bis-O-β-D-glucopyranoside(2 ),lariciresinol-4, 4'-bis-O-β-D-glucopyranoside(3 ),epigoitrin(4 ),indole-3-acetonitrile-6-O-β-D-glucopyranoside(5 ), 4-hydroxyindole-3-carboxaldehyde(6 ). Conclusion : For the first time, Compound 6 was isolated from Radix Isatidis.关键词：Radix Isatidis;water-soluble chemical composition;alkaloids
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective: To establish the quality standard of Bimintong Capsule. Method: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae were identified by TLC. The HPLC method was established to determine the content of osthole. A Shim-Pack ODS column was used with acetonitrile-water（60 ∶ 40） as mobile phases. The flow rate was 1. 0 mL·min -1. The detection wavelength was at 322 nm. Result: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae could be identified by TLC and there were noninterference of the negative controls. Osthole was linear in the range of 5. 68 ～ 56. 80 μg·mL -1 （ r = 0. 999 8）. The average recovery was 99. 27% ,and RSD 1. 01% （n = 6）. Conclusion:The method established can be used as quality control for Bushen Jianpi Granule.关键词：Bimintong Capsule;quality standard;TLC;HPLC;osthole
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- 65
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- 3
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for determination the content of Oleanic acid and Ursonic acid in crude hawthorn berry. Method: The HPLC analysis was carried out through C18 column with detection wavelength set at 210 nm and the temparature set at 35 ℃ . The mobile phase was composed of MeOHH2O-H3PO4-TEA （87∶ 13∶ 0. 2∶ 0. 04） at a flow rate of 0. 9 mL·min -1. Result:The separation of Oleanic acid and Ursonic acid was 1. 4; the methodologic validation conformed to requirement of liquid chromato-graphy. The content of Oleanic acid and Ursonic acid in crude hawthorn berry was more than 0. 5 mg·g -1 and 1. 9 mg·g -1, respectively. Conclusion:The HPLC method can be used in the routine analysis of Oleanic acid and Ursonic acid in crude hawthorn berry.关键词：Crude hawthorn berry;Oleanic acid;Ursonic acid;determination;HPLC
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- 485
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- 11
发布时间：2024-09-23 - 摘要：Objective: To establish the quality standard of Bimintong Capsule. Method: Rhizoma chuanxiong, Radix Angelicae pubescentis, and Radix saposhnikoviae were identified by TLC. The HPLC method was established to determine the content of osthole. A Shim-Pack ODS column was used with acetonitrile-water(60 ∶40) as mobile phases. The flow rate was 1.0 mL ·min-1. The detection wavelength was at 322 nm. Result: Rhizoma chuanxiong, Radix Angelicae pubescentis, and Radix saposhnikoviae could be identified by TLC and there were noninterference of the negative controls. Osthole was linear in the range of 5.68~56.80 μg ·mL-1(r=0.999 8).The average recovery was 99.27%, and RSD 1.01%(n=6). Conclusion: The method established can be used as quality control for Bushen Jianpi Granule.关键词：Bimintong Capsule;quality standard;TLC;HPLC;osthole
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To determine the content of Loganin in Ningxinanshen Particles by HPLC. Method: Phenomenex C18（4. 6 mm × 250 mm,5 μm） column was used. Acetonitrile-water by was used as a mobile phase gradient elution,flow rate was 1. 0 mL·min -1 and detection wavelength at 240 nm. Result:Loganin was linear in the rage of 0. 02 ～ 0. 40 μg Loganin,the correlation coefficient（ r） was 0. 999 9. The average recovery rate was 97. 10% ,RSD was 1. 14% . Conclusion:The method can be used for quality control of Ningxinanshen Particles.关键词：Loganin;Ningxinanshen Particles;HPLC
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- 62
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- 4
发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for determination the content of Oleanic acid and Ursonic acid in crude hawthorn berry. Method: The HPLC analysis was carried out through C18 column with detection wavelength set at 210 nm and the temparature set at 35 ℃. The mobile phase was composed of MeOH-H2O-H3PO4-TEA (87 ∶13 ∶0.2 ∶0.04)at a flow rate of 0.9 mL ·min-1. Result: The separation of Oleanic acid and Ursonic acid was 1.4; the methodologic validation conformed to requirement of liquid chromato-graphy. The content of Oleanic acid and Ursonic acid in crude hawthorn berry was more than 0.5 mg ·g-1 and 1.9 mg ·g-1, respectively. Conclusion: The HPLC method can be used in the routine analysis of Oleanic acid and Ursonic acid in crude hawthorn berry.关键词：Crude hawthorn berry;Oleanic acid;Ursonic acid;determination;HPLC
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:The chromatographic fingerprint of Polygonum Cuspidate was established and the content of polydatin,resveratrol and emodin was measured,which created a new method for evaluating and controlling the quality of Polygonum Cuspidate. Method:Using the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine （ Version2004A） recommended by State Food & Drug Administration, 10 different original samples were analyzed and the chromatographic fingerprint of Polygonum cuspidate was constructed with 13 common fingerprint peaks,among which three main fingerprint peaks of polydatin,resveratrol and emodin were identified by reversed-phase high performance liquid chromatography （ RP-HPLC）. Result:With the simple sample treating technique,the established fingerprint of Polygonum Cuspidate was stable and repeatable, which could be used to distinguish the superior polygonum cuspidate from the inferior polygonum cuspidate from different places and provide an important tool for further quality control of Polygonum Cuspidate.关键词：liquid chromatography （ LC）;fingerprint chromatography;Polygonum cuspidate;quality control
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- 481
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- 10
发布时间：2024-09-23 - 摘要：Objective: To determine the content of Loganin in Ningxinanshen Particles by HPLC. Method: Phenomenex C18(4.6 mm×250 mm,5 μm)column was used. Acetonitrile-water by was used as a mobile phase gradient elution, flow rate was 1.0 mL ·min-1 and detection wavelength at 240 nm. Result: Loganin was linear in the rage of 0.02~0.40 μg Loganin, the correlation coefficient(r) was 0.999 9. The average recovery rate was 97.10%,RSD was 1.14%. Conclusion: The method can be used for quality control of Ningxinanshen Particles.关键词：Loganin;Ningxinanshen Particles;HPLC
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:建立醒脑化瘀胶囊中人参皂苷Rg1、Rb1和三七皂苷R1的质量分数测定方法。方法:采用HPLC法同时测定醒脑化瘀胶囊中人参皂苷Rg1、Rb1和三七皂苷R1的质量分数。Gemini C18（4.6mm×250mm,5μm）色谱柱;乙腈、水为流动相,梯度洗脱;检测波长为203nm;流速为1.0mL·min-1。结果:人参皂苷Rg1、Rb1和三七皂苷R1分别在2.0600～10.3000μg、1.0740～5.3700μg及0.1994～0.9970μg范围内呈现良好的线性关系;回收率分别为97.81%、97.74%和97.33%,RSD分别为1.14%、1.10%和1.14%（n=6）。结论:该方法简便、准确,重现性好,可用于醒脑化瘀胶囊的质量控制。关键词：醒脑化瘀胶囊;人参皂苷Rg1、Rb1和三七皂苷R1;高效液相色谱法
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- 144
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- 3
发布时间：2024-09-23 - 摘要：Objective: The chromatographic fingerprint of Polygonum Cuspidate was established and the content of polydatin, resveratrol and emodin was measured, which created a new method for evaluating and controlling the quality of Polygonum Cuspidate. Method :Using the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine (Version2004A) recommended by State Food & Drug Administration, 10 different original samples were analyzed and the chromatographic fingerprint of Polygonum cuspidate was constructed with 13 common fingerprint peaks, among which three main fingerprint peaks of polydatin, resveratrol and emodin were identified by reversed-phase high performance liquid chromatography (RP-HPLC). Result :With the simple sample treating technique, the established fingerprint of Polygonum Cuspidate was stable and repeatable, which could be used to distinguish the superior polygonum cuspidate from the inferior polygonum cuspidate from different places and provide an important tool for further quality control of Polygonum Cuspidate.关键词：liquid chromatography (LC);fingerprint chromatography;Polygonum cuspidate;quality control
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:建立丹葛颈舒片中丹参酮ⅡA的含量测定方法。方法:采用高效液相色谱法,以phenomenex HyperClone色谱柱为分离柱,以甲醇-水-冰醋酸（78∶22∶0.2）为流动相,270nm为检测波长,柱温为室温。结果:丹参酮ⅡA在0.10～0.60μg范围内,峰面积与其浓度呈良好线性关系（r=0.9995）,平均回收率为102.0%,RSD为0.25%（n=5）。结论:该方法简便、准确、重复性好,可作为该制剂含量测定方法。关键词：丹葛颈舒片;丹参酮ⅡA;含量测定;高效液相法
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- 81
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- 7
发布时间：2024-09-23 - 摘要：Objective:To study the soluble calcium ion content of different compatibility of Baihu Decoction and to discuss the compatibility regularity of Baihu Decoction. Method:ICP-AES was used to detect the content of soluble calcium ion of each single and different compatibility herbs in Baihu Decoction. Result:The results showed that all of herbs in Baihu Decoction contained calcium. Gypsum had the highest content of calcium,followed by Rhizoma Anemarrhenae and Licoroce,Rice had the lowest. The calcium ion dissolution content was increased when Gypsum and Licoroce compatibled together; but not influenced by Gypsum and Rice and reduced by Gypsum and Rhizoma Anemarrhenae. Also the calcium ion content was increased when all herbs of Baihu Decoction compatibled together. Conclusion:It reflected the prescription principles of traditional Chinese medicine sufficiently. Gypsum is the monarch drug and Rhizoma Anemarrhenae is the minister drug,both of them have antipyretic effect. Licorice is assistant drug to restrain some Rhizoma Anemarrhenae’s negative effects on the calcium ion solubility of gypsum,and increase the antipyretic effect of the monarch drug and minister drug. So the compatibility of traditional Chinese medicine has its own regularity and rationality,it has important guiding significance to the further research on the pharmacological effects of mineral drugs in Chinese herbal compound.关键词：ICP-AES;Content of calcium ion solubility;Baihu Decoction;Gypsum;compatibility
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- 337
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- 27
发布时间：2024-09-23 - 摘要：Objective: To analyze and study the rule of prescription formulation based on the active components of the the Soup of Banxia （ Rhizoma Pinelliae）,Baishu （ Rhizoma Atractyloids Macrocephalae） and Tianma （ Rhizoma Gastrodiae）. Method: To take apart the soup according to the law of Monarch,Minister, Assistant and Guide,to determine the contents of gastrodin in Tianma which represents the monarch drug in different decomposed recipes using HPLC,and to research the essence of compatibility of the soup from the changes in gastrodin content. Result: The gastrodin in Tianma is 11. 4 mg /g,while the levels of gastrodin in Tianma + Banxia,in Tianma + Juhong,in Tianma + Gancao,in Tianma + Banxia + Baishu + Fuling + Juhong and in Tianma + Banxia + Baishu + Fuling + Juhong + Gancao are all higher than that in Tianma alone. Conclusion:Most of the drugs in the soup except Baishu and Fuling have various degree of promoter action for the extraction yield of gastrodin.关键词：Soup of Banxia,Baishu and Tianma;prescription formulation;gastrodin;determination
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- 806
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- 35
发布时间：2024-09-23 - 摘要：Objective: To study the soluble calcium ion content of different compatibility of Baihu Decoction and to discuss the compatibility regularity of Baihu Decoction. Method: ICP-AES was used to detect the content of soluble calcium ion of each single and different compatibility herbs in Baihu Decoction. Result: The results showed that all of herbs in Baihu Decoction contained calcium. Gypsum had the highest content of calcium, followed by Rhizoma Anemarrhenae and Licoroce, Rice had the lowest. The calcium ion dissolution content was increased when Gypsum and Licoroce compatibled together; but not influenced by Gypsum and Rice and reduced by Gypsum and Rhizoma Anemarrhenae.Also the calcium ion content was increased when all herbs of Baihu Decoction compatibled together. Conclusion: It reflected the prescription principles of traditional Chinese medicine sufficiently. Gypsum is the monarch drug and Rhizoma Anemarrhenae is the minister drug, both of them have antipyretic effect. Licorice is assistant drug to restrain some Rhizoma Anemarrhenaes negative effects on the calcium ion solubility of gypsum, and increase the antipyretic effect of the monarch drug and minister drug. So the compatibility of traditional Chinese medicine has its own regularity and rationality, it has important guiding significance to the further research on the pharmacological effects of mineral drugs in Chinese herbal compound.关键词：ICP-AES;Content of calcium ion solubility;Baihu Decoction;Gypsum;compatibility
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:对川芎药材、杭黄菊药材及川芎菊花复方提取物所得挥发油的化学成分进行比较。方法:使用气质联用（GC-MS）对提取物进行比较分析。结果:鉴定出川芎挥发油中的13个成分;杭黄菊挥发油中的10个成分;合提物挥发油中的14个成分。结论:药材的单独提取与复方合提其挥发性成分有较大的区别。关键词：川芎;杭黄菊;挥发油;气质联用
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- 214
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- 6
发布时间：2024-09-23 - 摘要：Objective: To analyze and study the rule of prescription formulation based on the active components of the the Soup of Banxia (Rhizoma Pinelliae), Baishu (Rhizoma Atractyloids Macrocephalae) and Tianma (Rhizoma Gastrodiae). Method :To take apart the soup according to the law of Monarch, Minister, Assistant and Guide, to determine the contents of gastrodin in Tianma which represents the monarch drug in different decomposed recipes using HPLC, and to research the essence of compatibility of the soup from the changes in gastrodin content. Result : The gastrodin in Tianma is 11.4 mg/g, while the levels of gastrodin in Tianma + Banxia, in Tianma +Juhong, in Tianma + Gancao, in Tianma+Banxia+Baishu+Fuling+ Juhong and in Tianma+Banxia+Baishu+Fuling+Juhong + Gancao are all higher than that in Tianma alone. Conclusion: Most of the drugs in the soup except Baishu and Fuling have various degree of promoter action for the extraction yield of gastrodin.关键词：Soup of Banxia,Baishu and Tianma;prescription formulation;gastrodin;determination
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective: To explore the trait variation of a popular cultivar ‘WEN85-5’ of Rehmannia glutinosa Libosoh. . Method: Comparison of main traits of four ‘WEN85-5 ’ samples, Taking Catalpo, Polysaccharides and aqueous extracts as the evaluation indices of R. glutinosa. quality; The Correlation of Analysis on the main traits and quality. Result: The morphological variation of ‘WEN85-5’was significant; The species are bred,its traits are decreasing on the fertility,anti-disease and the quality With the increasing years. Conclusion: the variation of traits have influenced the yield and the quality of Redix Rehmanniae; The farmer should introduce the first cultivar or recover its traits.关键词：Rehmannia glutinosa Libosoh.;‘WEN85-5’;variation of traits
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- 147
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- 3
发布时间：2024-09-23 - 摘要：Objective:To object the influence of a Chinese herbal compound granule with functions of cooling and promoting blood circulation on coagulation function and whole blood viscosity in rats with radiationinduced lung injury. Method:Ninety male wistar rats were randomly assigned to blank group,model group and treatment group with 30 rats in each group. The rats in model and treatment group were irradiated with 6 MV x-rays, 25Gy /fraction in the right lung and were given saline or Chinese herbal compound granule accordingly. The rats in blank group were given saline without radiation. Ten rats in each group were sacrificed at the time of 7 d,30 d and 90 d after radiation. The tissue pathology was conducted; Whole blood viscosity and coagulation indexes were evaluated. Result:The rats in model group developed radiation pneumonitis and fibrosis. The pathological changes of radiation injury in treatment group were slighter than the model group. The rats in model group were in the high coagulation and viscosity state in 7 d,in the low coagulation and viscosity state in 30 d,in the low coagulation and high viscosity state in 90 d. The coagulation time in the treatment group was longer in 7 d,shorter in 30 d and 90 d;The whole blood viscosity was decreased in 7 d,increased in 30 d,decreased in 90 d. Conclusion: Blood coagulation function disorder and viscosity abnormality occurred in the radiation-induced lung injury. The herbal compound granule with functions of cooling and promoting blood circulation can make appropriate adjustment and reduce the radiation-induced lung injury.关键词：radiation-induced lung injury;Chinese herbal medicine;blood cooling and promoting;whole blood viscosity
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- 175
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- 8
发布时间：2024-09-23 - 摘要：Objective: To study content variation of anthraquinones in Rhubarb before and after compatibilities with liquorice root, baikal skullcap root, red peony root, Chinese angelica, Chinese goldthread rhizome, costusroot and gardenia. Method: An HPLC method was established to determine the contents of free and glycoside-combined anthraquinones namely aloe-emodin, rhein,emodin,chrysophanol and physcion in rhubarb before and after compatibility. Result: The total anthraquinones were decreased after compatibility, especially when it was compatible with Chinese goldthread rhizome. Besides, the content of various anthraquinones was changed regularly after compatibility. Conclusion: The content of the five different anthraquinones was influenced by different compatibilities, which may contribute to decrease the toxicity of rhubarb.The study provides the basis when rhubarb is used in clinic.关键词：Rhubarb;compatibility;HPLC;anthraquinones
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- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the influence of PRD（Pu Ren Dan） ultramicro-powder on the functional indexes correlated with glycometabolism in T2DM rats,including plasma glucose,glucose tolerance,glycosylated serum protein,serum insulin and insulin sensitivity index. And to investigate the hypoglycemic effect of PRD. Method: Wistar rats were fed fat milk,and small doses of streptozotocin was rapidly injected into tail vein. Animal models of type 2 diabetes with macroangiopathy characterized by insulin resistance were successfully prepared in a short time. The model rats were randomly divided into five groups including PRD low dose,PRD medium dose,PRD high dose,rosiglitazone maleate and model groups. Every group was respectively treated for 4 weeks with PRD low dose,PRD medium dose,PRD high dose,rosiglitazone maleate and water. Random plasma glucose of rats were respectively measured before treatment,after 2 weeks and 4 weeks with treatment. Fasting serum insulin and glycosylated serum protein content of rats were measured on the 4th weekend. And Insulin sensitivity index were calculated. Result:As compared with normal group rats,random plasma glucose and glycosylated serum protein content of model group rats were increased,while serum insulin and insulin sensitivity index significantly were decreased（P < 0. 01）. Random plasma glucose and glycosylated serum protein content of rats were all significantly reduced in PRD low,medium,high dose and rosiglitazone maleate groups （ P < 0. 05 ～ 0. 01 ）. Serum insulin content and insulin sensitivity index of T2DM model rats treated by PRD medium,high dose and rosiglitazone maleate were significantly increased（ P < 0. 05 ～ 0. 01）. Conclusion:Based on reducing the high level of plasma glucose and glycosylated serum protein content,PRD ultramicro-powder can enhance insulin sensitivity of T2DM rats and improve glycometabolism in T2DM rats.关键词：type 2 diabetes mellitus;Pu Ren Dan ultramicro-powder;glycometabolism;insulin sensitivity
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- 325
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- 20
发布时间：2024-09-23 - 摘要：Objective: To explore the trait variation of a popular cultivar 'WEN85-5’ of Rehmannia glutinosa Libosoh.. Method: Comparison of main traits of four 'WEN85-5’ samples, Taking Catalpo, Polysaccharides and aqueous extracts as the evaluation indices of R. glutinosa. quality; The Correlation of Analysis on the main traits and quality. Result: The morphological variation of 'WEN85-5’ was significant; The species are bred, its traits are decreasing on the fertility, anti-disease and the quality With the increasing years. Conclusion: the variation of traits have influenced the yield and the quality of Redix Rehmanniae; The farmer should introduce the first cultivar or recover its traits.
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- 0
发布时间：2024-09-23 - 摘要：Objective: The inflammatory model in vitro was used to evaluate the anti-inflammatory mechanism of total saponins of Radix Glycyrrhiza. Method: RAW264. 7 macrophage line in mice was induced by LPS to set up the inflammatory model. To detect the effect of total saponins of Radix Glycyrrhiza on the release of inflammation factors and the key enzymes for arachidonic acid （AA） metabolism in synthesizing PGE2. Result: The total saponins of Radix Glycyrrhiza could significantly reduce the release of NO,IL-1 and TNF-α in LPS-induced RAW264. 7 macrophage line,and obviously decrease the synthesis of PGE2 by inhibiting PLA2 activity and the expression of COX-2. Conclusion: The anti-inflammatory mechanism of total saponins of Radix Glycyrrhiza may be related to reducing the release of inflammation factors in macrophage cell and inhibiting the key enzymes for AA metabolism pathway in synthesizing PGE2.关键词：total saponins of Radix Glycyrrhiza;NO;TNF-α;IL-1;PGE2;COX-2;PLA2
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- 1822
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- 171
发布时间：2024-09-23 - 摘要：Objective: To object the influence of a Chinese herbal compound granule with functions of cooling and promoting blood circulation on coagulation function and whole blood viscosity in rats with radiation-induced lung injury. Method: Ninety male wistar rats were randomly assigned to blank group, model group and treatment group with 30 rats in each group. The rats in model and treatment group were irradiated with 6 MV x-rays,25Gy/fraction in the right lung and were given saline or Chinese herbal compound granule accordingly. The rats in blank group were given saline without radiation. Ten rats in each group were sacrificed at the time of 7 d, 30 d and 90 d after radiation. The tissue pathology was conducted;Whole blood viscosity and coagulation indexes were evaluated. Result: The rats in model group developed radiation pneumonitis and fibrosis. The pathological changes of radiation injury in treatment group were slighter than the model group. The rats in model group were in the high coagulation and viscosity state in 7 d, in the low coagulation and viscosity state in 30 d, in the low coagulation and high viscosity state in 90 d. The coagulation time in the treatment group was longer in 7 d, shorter in 30 d and 90 d;The whole blood viscosity was decreased in 7 d, increased in 30 d, decreased in 90 d. Conclusion: Blood coagulation function disorder and viscosity abnormality occurred in the radiation-induced lung injury. The herbal compound granule with functions of cooling and promoting blood circulation can make appropriate adjustment and reduce the radiation-induced lung injury.关键词：radiation-induced lung injury;Chinese herbal medicine;blood cooling and promoting;whole blood viscosity
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发布时间：2024-09-23 - 摘要：Objective: To study the repairing effects of Buyang Huanwu Decoction （ BYHWD） on rat common peroneal nerve after peripheral nerve injury. Method: Common peroneal nerve injury induced by clamp was established for sixty Sprague-Dawley rats,then they were randomly divided into 6 groups for daily intragastric administration of drugs: BYHWD high-dose （25. 92 g /kg）,BYHWD medium-dose （12. 96 g /kg）,BYHWD lowdose （6. 48 g /kg）,mecobalamin （625 μg /kg） as the positive control,normal saline for model group and normal saline for the sham operation group. The drug administration lasted for 18 days. Electrophysiology was performed 18 days after operation to determine the compound muscle action potentials （ CMAPs）. The number of regenerated myelinated nerve fibers,thickness of myelin sheath and cross sectional area of tibial muscle were evaluated by histomorphology. Result: Amplitude of CMAP,the number of regenerated myelinated nerve fibers and thickness of myelin sheath in BYHWD high-dose and medium-dose group were all increased significantly compared with the model group. Conclusion: BYHWD contributes to axon growth and myelination reformation, thus promotes peripheral nerve regeneration in rats.关键词：peripheral nerve regeneration;Buyang Huanwu Decoction;common peroneal nerve;the compound muscle action potentials;histomorphology
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- 227
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- 21
发布时间：2024-09-23 - 摘要：Objective: To observe the influence of PRD(Pu Ren Dan) ultramicro-powder on the functional indexes correlated with glycometabolism in T2DM rats,including plasma glucose,glucose tolerance,glycosylated serum protein, serum insulin and insulin sensitivity index.And to investigate the hypoglycemic effect of PRD. Method: Wistar rats were fed fat milk,and small doses of streptozotocin was rapidly injected into tail vein.Animal models of type 2 diabetes with macroangiopathy characterized by insulin resistance were successfully prepared in a short time.The model rats were randomly divided into five groups including PRD low dose,PRD medium dose,PRD high dose,rosiglitazone maleate and model groups. Every group was respectively treated for 4 weeks with PRD low dose, PRD medium dose,PRD high dose,rosiglitazone maleate and water. Random plasma glucose of rats were respectively measured before treatment,after 2 weeks and 4 weeks with treatment. Fasting serum insulin and glycosylated serum protein content of rats were measured on the 4th weekend.And Insulin sensitivity index were calculated. Result: As compared with normal group rats, random plasma glucose and glycosylated serum protein content of model group rats were increased,while serum insulin and insulin sensitivity index significantly were decreased(P<0.01). Random plasma glucose and glycosylated serum protein content of rats were all significantly reduced in PRD low, medium, high dose and rosiglitazone maleate groups (P<0.05~0.01).Serum insulin content and insulin sensitivity index of T2DM model rats treated by PRD medium, high dose and rosiglitazone maleate were significantly increased(P<0.05~0.01). Conclusion: Based on reducing the high level of plasma glucose and glycosylated serum protein content, PRD ultramicro-powder can enhance insulin sensitivity of T2DM rats and improve glycometabolism in T2DM rats.关键词：type 2 diabetes mellitus;Pu Ren Dan ultramicro-powder;glycometabolism;insulin sensitivity
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology,structure,function and protein of mitochondria. Method: Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM + Weichangshu Ⅰ group, DM + Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential,respiratory chain and mitochondrial protein were detected using flow cytometry,fluorescence labeling and Western Blot respectively. Result: Weichangshu could ameliorate the mitochondrial morphology,structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential,ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion:Weichangshu could promoted the gastrointestinal motility,which might be related with the regulation of mitochondrial kinetic energy.关键词：chinese medicine Weichangshu;gastrointestinal motility;mitochondrial;transmembrane potential;respiratory chain
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- 183
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- 9
发布时间：2024-09-23 - 摘要：Objective: The inflammatory model in vitro was used to evaluate the anti-inflammatory mechanism of total saponins of Radix Glycyrrhiza. Method: RAW264.7 macrophage line in mice was induced by LPS to set up the inflammatory model. To detect the effect of total saponins of Radix Glycyrrhiza on the release of inflammation factors and the key enzymes for arachidonic acid (AA) metabolism in synthesizing PGE2. Result: The total saponins of Radix Glycyrrhiza could significantly reduce the release of NO, IL-1 and TNF-α in LPS-induced RAW264.7 macrophage line, and obviously decrease the synthesis of PGE2 by inhibiting PLA2 activity and the expression of COX-2. Conclusion: The anti-inflammatory mechanism of total saponins of Radix Glycyrrhiza may be related to reducing the release of inflammation factors in macrophage cell and inhibiting the key enzymes for AA metabolism pathway in synthesizing PGE2.关键词：total saponins of Radix Glycyrrhiza;NO;TNF-α;IL-1;PGE2;COX-2;PLA2
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective: To explore the pharmacokinetic of Sinomenine transdermal patch. Method: The plasma drug concentration of rats arthritis model induced by Freund’s adjuvant was determined after administration with HPLC-UVD as analysis tools. The pharmacokinetics parameters was fitted with kinetica software package. Result: The exclusive analysis method was established with the following pharmacokinetics parameters: T（peak） = 10 h,Cmax = 455 ng /mL,MRT = 24 h. The pharmacokinetic characteristics was accordance with one-order rate and two – compartment model. Conclusion:The method is suitable to be applied to the pharmacokinetics research and further applied pharmacological study which will play a reference role in clinical application.关键词：Sinomenine patch;arthritis rats model induced by Freund’s adjuvant;pharmacokinetics
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- 245
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- 3
发布时间：2024-09-23 - 摘要：Objective: To study the repairing effects of Buyang Huanwu Decoction(BYHWD) on rat common peroneal nerve after peripheral nerve injury. Method : Common peroneal nerve injury induced by clamp was established for sixty Sprague-Dawley rats, then they were randomly divided into 6 groups for daily intragastric administration of drugs: BYHWD high-dose (25.92 g/kg), BYHWD medium-dose (12.96 g/kg), BYHWD low-dose (6.48 g/kg), mecobalamin (625 μg/kg) as the positive control, normal saline for model group and normal saline for the sham operation group. The drug administration lasted for 18 days. Electrophysiology was performed 18 days after operation to determine the compound muscle action potentials (CMAPs). The number of regenerated myelinated nerve fibers, thickness of myelin sheath and cross sectional area of tibial muscle were evaluated by histomorphology. Result : Amplitude of CMAP, the number of regenerated myelinated nerve fibers and thickness of myelin sheath in BYHWD high-dose and medium-dose group were all increased significantly compared with the model group. Conclusion : BYHWD contributes to axon growth and myelination reformation, thus promotes peripheral nerve regeneration in rats.关键词：peripheral nerve regeneration;Buyang Huanwu Decoction;common peroneal nerve;the compound muscle action potentials;histomorphology
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:HPLC/MS/MS-based study on the changes of endogenous metabolites of Sprague-Dawley Rats administrated with water extract of Zanthoxylumschinifolium Siebet Zucc. Method:Ten Sprague-Dawley rats were successively administrated with Zanthoxylumschinifolium Siebet Zucc water extract （0. 342 8 g·kg -1 ） for 30 days. Agilent1200 6410 Triple Quadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data were analyzed by OSC-PLS-DA. Result: The difference in metabolic profiles between the control group and the dosed group was well observed by OSC-PLS-DA analysis after administration of 15 days,31 biomarkers were found, such as cytidine, decanoylcarnitine and N-acetylglucosamine 6-phosphate. Conclusion:The administration of Zanthoxylumschinifolium Siebet Zucc water extract could influence concentrations of endogenous matabolites related to anticancer,analgesic and hypolipidemic pharmacological actions. This work can provide basis for further researches on the interpretation of drug property of evodiae.关键词：metabonmontics;Zanthoxylumschinifolium Siebet Zucc;biomarkers
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- 927
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- 18
发布时间：2024-09-23 - 摘要：Objective: To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology, structure, function and protein of mitochondria. Method : Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM+ WeichangshuⅠgroup, DM+ Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential, respiratory chain and mitochondrial protein were detected using flow cytometry, fluorescence labeling and Western Blot respectively. Result :Weichangshu could ameliorate the mitochondrial morphology, structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential, ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion :Weichangshu could promoted the gastrointestinal motility, which might be related with the regulation of mitochondrial kinetic energy.关键词：chinese medicine Weichangshu;gastrointestinal motility;mitochondrial;transmembrane potential;respiratory chain
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To study the intestinal mechanism of Huoxiang Zhengqi Liquid （HZL）. Method: The spontaneous contraction of colonic muscle from mice were studied using muscle tension measurement technology. Result: HZL could inhibit the spontaneous contraction caused by CCH or KCl of colonic muscle from mice. Conclusion: The effect of HZL on the spontaneous contraction is related with inhibition of Ca2 + channel.关键词：Huoxiang Zhengqi Liquid;intestinal;mechanism;muscle tension
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- 329
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- 3
发布时间：2024-09-23 - 摘要：Objective: HPLC/MS/MS-based study on the changes of endogenous metabolites of Sprague-Dawley Rats administrated with water extract of Zanthoxylumschinifolium Siebet Zucc. Method: Ten Sprague-Dawley rats were successively administrated with Zanthoxylumschinifolium Siebet Zucc water extract (0.342 8 g ·kg-1) for 30 days. Agilent1200 6410 Triple Quadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data were analyzed by OSC-PLS-DA. Result: The difference in metabolic profiles between the control group and the dosed group was well observed by OSC-PLS-DA analysis after administration of 15 days, 31 biomarkers were found, such as cytidine, decanoylcarnitine and N-acetylglucosamine 6-phosphate. Conclusion: The administration of Zanthoxylumschinifolium Siebet Zucc water extract could influence concentrations of endogenous matabolites related to anticancer, analgesic and hypolipidemic pharmacological actions. This work can provide basis for further researches on the interpretation of drug property of evodiae.关键词：metabonmontics;Zanthoxylumschinifolium Siebet Zucc;biomarkers
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Yizhiyin on learning and memory ability in the rats with Alzheimer’s disease induced by Aβ1-42 and the effect of antioxidation. Method:The rat model of AD were established by Aβ1-42 injection into hippocampus,then those rats were treated by three different doses of Yizhiyin. Morris watermaze test was used for detecting learning and memory ability. Immunohistochemisty was used for detecting the expression of β-Amyloid,and the superoxide dismutase （ SOD） and the hydroxy radical inhibition of brain were measured. Result:Compared with model group,the ability of learning and memory in high,middle and low doses group were improved （ P < 0. 01）. The results of immunohistochemisty showed that there were a lot of β-Amyloid deposition in the brain of model rats and a sprinkle expression in high and middle doses group. The activity of SOD was increased compared to those in model group in brain in each treatment group （ P < 0. 01）,the inhibition of hydroxy radical was increased （ P < 0. 01） in each treatment group except the low dose group. Conclusion:The Yizhiyin could improve the learning and memory capability in AD rats efficiently,and decrease the β-Amyloid deposition. The mechanism of the Yizhiyin should be related to the increase in SOD and the inhibition of hydroxy radical.关键词：Yizhiyin;Alzheimer’s disease;learning and memory ability;antioxidation
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- 245
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- 10
发布时间：2024-09-23 - 摘要：Objective: To study the intestinal mechanism of Huoxiang Zhengqi Liquid (HZL). Method : The spontaneous contraction of colonic muscle from mice were studied using muscle tension measurement technology. Result : HZL could inhibit the spontaneous contraction caused by CCH or KCl of colonic muscle from mice. Conclusion: The effect of HZL on the spontaneous contraction is related with inhibition of Ca2+ channel.关键词：Huoxiang Zhengqi Liquid;intestinal;mechanism;muscle tension
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To observe and to discuss the effect of Liqi Tiaobu Tang on hypothalamic-pituitaryadrenal axis in the rat of fatigue induced by exercise. Method:30 male SD rats were randomly divided into 3 groups. Ⅰ and Ⅱ groups are all trained for 6 weeks with swimming. The last exhaustive swimming time,serum corticosterone,testosterone,and hypothalamic β-endorphin were observed. Result:Liqi Tiaobu Tang could extend the time for exhaustive swimming in rats（P < 0. 01）,increase the content of T,and decrease the content of serum β-endorphin. Conclusion:Liqi Tiaobu Tang improves hypothalamus-pituitary-adrenal axis function in the rat of fatigue induced by exercise. It may prevent the exercise fatigue.关键词：Liqi Tiaobu Tang;exercise-induced fatigue;hypothalamic-pituitary-adrenal axis
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- 196
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- 12
发布时间：2024-09-23 - 摘要：Objective: To observe the effect of Yizhiyin on learning and memory ability in the rats with Alzheimers disease induced by Aβ1-42 and the effect of antioxidation. Method: The rat model of AD were established by Aβ1-42 injection into hippocampus, then those rats were treated by three different doses of Yizhiyin. Morris watermaze test was used for detecting learning and memory ability. Immunohistochemisty was used for detecting the expression of β-Amyloid, and the superoxide dismutase (SOD) and the hydroxy radical inhibition of brain were measured. Result: Compared with model group, the ability of learning and memory in high,middle and low doses group were improved (P<0.01).The results of immunohistochemisty showed that there were a lot of β-Amyloid deposition in the brain of model rats and a sprinkle expression in high and middle doses group. The activity of SOD was increased compared to those in model group in brain in each treatment group (P<0.01), the inhibition of hydroxy radical was increased (P<0.01) in each treatment group except the low dose group. Conclusion: The Yizhiyin could improve the learning and memory capability in AD rats efficiently, and decrease the β-Amyloid deposition. The mechanism of the Yizhiyin should be related to the increase in SOD and the inhibition of hydroxy radical.关键词：Yizhiyin;Alzheimers disease;learning and memory ability;antioxidation
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the immunohistochemical expression of interferon-γ（IFN-γ）,transforming growth factor-β （ TGF-β）,matrix metalloproteinase-9 （ MMP-9） and lymphocyte subsets in brain and spinal cord tissue of experimental autoimmune encephalomyelitis（ EAE） rats,and explore the mechanism of Erhuang Capsule,comparing the differences in action for the two dosage forms（ capsule and decoction）. Method:Lewis rats were immunized with the myelin basic protein （ MBP）. Rats were divided randomly into nomal-group,EAE-group, prednisone-group,Erhuang Decoction group and Erhuang Capusule group after post immunization （PI）. On the day 15th and the day 27th of PI,rats were killed and the immunohistochemical staining of IFN-γ、TGF-β、MMP-9、CD4 + 、 CD8 + 、CD3 + and CD19 + was performed on the sections of brain and spinal cord. Result:Compared with the EAE- group,Erhuang Decoction （ on day 27th PI） and Erhuang Capusule （ on day 15 and 27 PI） both can raise the expression of TGF-β in brain tissue. Erhuang Decoction,Erhuang Capusule and prednisone can decrease the expression of IFN-γ and MMP-9 in brain tissue and spinal cord tissue on day 15th and 27th PI;compared with the prednisone,Erhuang Decoction and Erhuang Capusule can decrease the expression of IFN-γ in brain tissue and spinal cord tissue on day 15th and 27th PI,they can also decrease the expression of MMP-9 in brain tissue and spinal cord tissue on day 15th PI. Compared with the EAE-group,the expresstion of CD4 + in brain tissue of Erhuang Decoction group was significantly lower than that of EAE-group on day 15th and 27th PI,while the expresstion of CD8 + raised and CD4 + /CD8 + decreased（ The expresstion of CD19 + decreased on day 15th PI）. On day 15th PI, there is no difference in the expression of CD8 + between the Erhuang Capusule group and EAE-group,others were the same as Erhuang Decoction group. Compared with the EAE-group,the expresstion of CD4 + in spinal cord tissue of Erhuang Capusule group was significantly lower than that of EAE-group on day 15th,while the expresstion of CD8 + raised and CD4 + /CD8 + decreased,on day 27th PI,the expresstion of CD4 + and CD4 + /CD8 + was decreased. There was no statistical difference between Erhuang Decoction and Erhuang Capusule. Conclusion: Erhuang Decoction and Erhuang Capusule both have the effect on upregulating the immunohistochemical expression of TGF-β in central nervous system of EAE rats,playing the same role as prednisone. They can also downregulate the expression of IFN-γ and MMP-9,are better than prednisone. Erhuang Decoction and Erhuang Capusule also can downregulate the expression of CD4 + and upregulate the expression of CD8 + ,to improve the ratio of CD4 + /CD8 + . The impact on indexs described above may be the functional mechanism of Erhuang Decoction and Erhuang Capusule. There was no significant difference between Erhuang Decoction and Erhuang Capusule. [关键词：] autoimmune diseases;experimental autoimmune encephalomyelitis;cytokines;Erhuang Decoction;Erhuang Capusule
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- 316
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- 17
发布时间：2024-09-23 - 摘要：Objective: To observe and to discuss the effect of Liqi Tiaobu Tang on hypothalamic-pituitary-adrenal axis in the rat of fatigue induced by exercise. Method: 30 male SD rats were randomly divided into 3 groups. Ⅰ and Ⅱ groups are all trained for 6 weeks with swimming. The last exhaustive swimming time,serum corticosterone,testosterone, and hypothalamic β-endorphin were observed. Result: Liqi Tiaobu Tang could extend the time for exhaustive swimming in rats(P<0.01),increase the content of T, and decrease the content of serum β-endorphin. Conclusion: Liqi Tiaobu Tang improves hypothalamus-pituitary-adrenal axis function in the rat of fatigue induced by exercise. It may prevent the exercise fatigue.关键词：Liqi Tiaobu Tang;exercise-induced fatigue;hypothalamic-pituitary-adrenal axis
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective: To explore the effect and mechanism of breviscapine against myocardial ischemia/ reperfusion injury in rat. Method: 40 SD rats were randomly divided into sham operation group,ischemia / reperfusion group and breviscapine group. Rats in breviscapine group were injected with breviscapine 25,50 mg· kg1· d -1 respectively prior to ischemia /reperfusion. The other two groups were given normal saline instead of breviscapine equally in volume at a week before the operation. The myocardial ischemia /reperfusion model was established by ligating the left anterior descending branch of coronary artery. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with Annexin Ⅴ and propidium iodiode （ PI ） staining. The ultrastructural alterations were observed under the transmission electron microscopy. Result: breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia /reperfusion injury obviously（ P < 0. 05,P < 0. 01）. The degree of injury on ultrastructures of myocardial cell in breviscapine group is rather alleviated compared with that in the ischemia /reperfusion group. Conclusion: breviscapine may protect myocardial cells during ischemia /reperfusion injury and the protective mechanism may be achieved by inhibiting myocardial apoptosis.关键词：breviscapine;apoptosis;myocardium;ischemia/reperfusion;ultrastructures
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- 251
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- 13
发布时间：2024-09-23 - 摘要：Objective: To observe the immunohistochemical expression of interferon-γ(IFN-γ),transforming growth factor-β(TGF-β),matrix metalloproteinase-9(MMP-9) and lymphocyte subsets in brain and spinal cord tissue of experimental autoimmune encephalomyelitis(EAE) rats,and explore the mechanism of Erhuang Capsule,comparing the differences in action for the two dosage forms(capsule and decoction). Method: Lewis rats were immunized with the myelin basic protein (MBP). Rats were divided randomly into nomal-group,EAE-group,prednisone-group,Erhuang Decoction group and Erhuang Capusule group after post immunization (PI). On the day 15th and the day 27th of PI,rats were killed and the immunohistochemical staining of IFN-γ、TGF-β、MMP-9、CD4+、CD8+、CD3+ and CD19+was performed on the sections of brain and spinal cord. Result: Compared with the EAE-group,Erhuang Decoction (on day 27th PI)and Erhuang Capusule(on day 15 and 27 PI)both can raise the expression of TGF-β in brain tissue. Erhuang Decoction, Erhuang Capusule and prednisone can decrease the expression of IFN-γ and MMP-9 in brain tissue and spinal cord tissue on day 15th and 27th PI;compared with the prednisone,Erhuang Decoction and Erhuang Capusule can decrease the expression of IFN-γ in brain tissue and spinal cord tissue on day 15th and 27th PI,they can also decrease the expression of MMP-9 in brain tissue and spinal cord tissue on day 15th PI. Compared with the EAE-group,the expresstion of CD4+in brain tissue of Erhuang Decoction group was significantly lower than that of EAE-group on day 15th and 27th PI,while the expresstion of CD8+raised and CD4+/CD8+decreased(The expresstion of CD19+ decreased on day 15th PI). On day 15th PI,there is no difference in the expression of CD8+between the Erhuang Capusule group and EAE-group,others were the same as Erhuang Decoction group. Compared with the EAE-group,the expresstion of CD4+in spinal cord tissue of Erhuang Capusule group was significantly lower than that of EAE-group on day 15th,while the expresstion of CD8+raised and CD4+/CD8+decreased,on day 27th PI,the expresstion of CD4+and CD4+/CD8+ was decreased. There was no statistical difference between Erhuang Decoction and Erhuang Capusule. Conclusion: Erhuang Decoction and Erhuang Capusule both have the effect on upregulating the immunohistochemical expression of TGF-β in central nervous system of EAE rats,playing the same role as prednisone. They can also downregulate the expression of IFN-γ and MMP-9,are better than prednisone. Erhuang Decoction and Erhuang Capusule also can downregulate the expression of CD4+and upregulate the expression of CD8+,to improve the ratio of CD4+/CD8+. The impact on indexs described above may be the functional mechanism of Erhuang Decoction and Erhuang Capusule. There was no significant difference between Erhuang Decoction and Erhuang Capusule.关键词：autoimmune diseases;experimental autoimmune encephalomyelitis;cytokines;Erhuang Decoction;Erhuang Capusule
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of Huangjin capsule on insulin sensitivity in type 2 diabetic rats with insulin-resistance （ IR） and the primary pharmacological mechanism. Method: The model for type 2 diabetic-insulin-resistant rats （ T2DIRR ） were performed through intraperitonea injection of lower dosage of streptozocin（30 mg /kg） and high caloric diet （ abounded with sugar & fat）. The effects of Huangjin on weight, blood and /or serum contents of glucose,lipids,insulin and insulin sensitive index（ISI） were observed in the model rats. Result: Huangjin lowered significantly fasting blood glucose （ FBG） （ P < 0. 05 ） and improved impaired glucose tolerance （IgT）; and reduced the concentration of insulin in hyperinsulinemia,and potentiated ISI （ P < 0. 01） in T2DIRR. Conclusion: In the experiment with type 2 diabetic-insulin-resistantrats,Huangjin can reduce the level of blood glucose,and improve insulin sensitivity.关键词：Huangjin capsule;type 2 diabetes mellitus;insulin resistance;animal model;insulin sensitivity improver
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- 144
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发布时间：2024-09-23 - 摘要：Objective: To explore the effect and mechanism of breviscapine against myocardial ischemia/reperfusion injury in rat. Method : 40 SD rats were randomly divided into sham operation group, ischemia/reperfusion group and breviscapine group. Rats in breviscapine group were injected with breviscapine 25, 50 mg·kg1·d-1 respectively prior to ischemia/reperfusion. The other two groups were given normal saline instead of breviscapine equally in volume at a week before the operation.The myocardial ischemia/reperfusion model was established by ligating the left anterior descending branch of coronary artery. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with AnnexinⅤand propidium iodiode (PI) staining. The ultrastructural alterations were observed under the transmission electron microscopy. Result: breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia/reperfusion injury obviously(P<0.05,P<0.01). The degree of injury on ultrastructures of myocardial cell in breviscapine group is rather alleviated compared with that in the ischemia/reperfusion group. Conclusion: breviscapine may protect myocardial cells during ischemia/reperfusion injury and the protective mechanism may be achieved by inhibiting myocardial apoptosis.关键词：breviscapine;apoptosis;myocardium;ischemia/reperfusion;ultrastructures
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发布时间：2024-09-23 - 摘要：Aim:To study the protective therapeutic actions and mechanism of Liuwei Dihuang Decoction, Buzhong Yiqi Decoction and Compound Danshen Decoction on the marrow-suppressed mice. Method: The expression of thrombopoietin （ TPO ） mRNA,thrombopoietin receptor （ c-Mpl） mRNA and GATA-1 mRNA was detected by RT-PCR technique. Result: Liuwei Dihuang Decoction and Buzhong Yiqi Decoction were shown to promote the expression of TPO mRNA,c-Mpl mRNA and GATA-1 mRNA in spleen of mice. The result indicates the decoctions could accelerate the marrow cells of the marrow-suppressed mice. Although Compound Danshen Decoction did not show significant promotion for the expression of TPO mRNA,c-Mpl mRNA and GATA-1 mRNA in spleen of mice,but it also could accelerate peripheral blood cells of the marrow-suppressed mice. Conclusion: Peripheral blood cells of marrow-suppressed mice were increased significantly by Liuwei Dihuang Decoction, Buzhong Yiqi Decoction and Compound Danshen Decoction.关键词：Liuwei Dihuang Decoction;Buzhong Yiqi Decoction;Compound Danshen Decotion;marrow suppress;TPO mRNA;c-Mpl mRNA;GATA-1 mRNA
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- 355
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- 19
发布时间：2024-09-23 - 摘要：Objective: To investigate the effects of Huangjin capsule on insulin sensitivity in type 2 diabetic rats with insulin-resistance (IR) and the primary pharmacological mechanism. Method: The model for type 2 diabetic-insulin-resistant rats (T2DIRR) were performed through intraperitonea injection of lower dosage of streptozocin(30 mg/kg) and high caloric diet (abounded with sugar & fat). The effects of Huangjin on weight, blood and/or serum contents of glucose, lipids,insulin and insulin sensitive index(ISI) were observed in the model rats. Result: Huangjin lowered significantly fasting blood glucose (FBG)(P<0.05) and improved impaired glucose tolerance (IgT); and reduced the concentration of insulin in hyperinsulinemia, and potentiated ISI (P<0.01) in T2DIRR. Conclusion: In the experiment with type 2 diabetic-insulin-resistantrats, Huangjin can reduce the level of blood glucose, and improve insulin sensitivity.关键词：Huangjin capsule;type 2 diabetes mellitus;insulin resistance;animal model;insulin sensitivity improver
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发布时间：2024-09-23 - 摘要：Objective:To investigate the anti-angiogenic mechanism of QHF （a formula of Chinese medicine ingredients,abbreviation Q for Qingrejiedu,H for Huoxuehuayu and F for Fuzhengguben） combined with small dose chemotherapy drug cisplatin（DDP）in H22 mice of hepatocellular carcinoma. Method: The forty eight Balb /c mice were inoculated with cell suspension of mouse hepatocellular carcinoma cell line H22 under the right axillary skin to establish the solid tumor model. Then they were randomly divided into four groups as follows: QHF group, DDP group,QHF + DDP group and NS （ normal saline） group. The inhibition rates of tumor growth in tumorbearing mice by drugs as an indicator were observed. The expression of VEGF,EGFR and MMP-2 in the tissue of mice tumor was detected with immunohistochemisty. Result:The weight of tumor was significantly lower in QHF, DDP and QHF + DDP group than that of in NS group（P < 0. 01）,while the weight of mice tumor was significantly lower in QHF + DDP group than that of in QHF group. Their inhibition rate was 47. 45% ,63. 11% ,72. 95% , respectively. The expression of VEGF and MMP-2 in the tissue of mice tumor was significantly lower in QHF,DDP and QHF + DDP group than that of in NS group（P < 0. 05,P < 0. 01 and P < 0. 01）. Moreover,The expression of EGFR in the tissue of mice tumor was significantly lower in QHF,DDP and QHF + DDP group than that of in NS group（P < 0. 01,P < 0. 05 and P < 0. 01）. However,the difference between QHF + DDP group and other groups has no significance （ P > 0. 05）. Conclusion: Both of QHF and small dose DDP have the anti-tumor and antiangiogenic effect in H22 mice of hepatocellular carcinoma. Moreover,one of anti-angiogenic mechanism may be concerned with the downregulation of the expression of VEGF,EGFR and MMP-2 in the tissue of mice tumor.关键词：active ingredients;hepatic cell cancer;cisplatin;angiogenesis;mechanism
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- 459
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发布时间：2024-09-23 - 摘要：Aim: To study the protective therapeutic actions and mechanism of Liuwei Dihuang Decoction,Buzhong Yiqi Decoction and Compound Danshen Decoction on the marrow-suppressed mice. Method :The expression of thrombopoietin(TPO)mRNA, thrombopoietin receptor(c-Mpl)mRNA and GATA-1 mRNA was detected by RT-PCR technique. Result: Liuwei Dihuang Decoction and Buzhong Yiqi Decoction were shown to promote the expression of TPO mRNA, c-Mpl mRNA and GATA-1 mRNA in spleen of mice. The result indicates the decoctions could accelerate the marrow cells of the marrow-suppressed mice. Although Compound Danshen Decoction did not show significant promotion for the expression of TPO mRNA, c-Mpl mRNA and GATA-1 mRNA in spleen of mice, but it also could accelerate peripheral blood cells of the marrow-suppressed mice. Conclusion: Peripheral blood cells of marrow-suppressed mice were increased significantly by Liuwei Dihuang Decoction,Buzhong Yiqi Decoction and Compound Danshen Decoction.关键词：Liuwei Dihuang Decoction;Buzhong Yiqi Decoction;Compound Danshen Decotion;marrow suppress;TPO mRNA;c-Mpl mRNA;GATA-1 mRNA
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发布时间：2024-09-23 - 摘要：目的:探讨陈皮、藿香不同提取物对肢体缺血-再灌注（LIR）大鼠胃肠动力的影响。方法:采用LIR模型,观察陈皮水提液、陈皮苷及藿香水提液、藿香水提液+挥发油、百秋里醇对LIR大鼠胃肠运动的影响。结果:LIR大鼠胃排空和小肠推进能力较空白对照组均明显降低（均P<0.01）。陈皮水提液、陈皮苷、藿香水提液、藿香水提液+挥发油均能明显增强LIR模型大鼠胃排空和小肠推进能力（均P<0.01）,百秋里醇则对其无影响;陈皮水提液与陈皮苷两组间、藿香水提液与藿香水提液+挥发油两组间胃排空和小肠推进状况差异均具有统计学意义（均P<0.01）。结论:对于改善LIR模型大鼠胃排空和小肠推进能力,陈皮水提液多组分优于陈皮苷单组分,藿香水提液+挥发油全组分优于藿香水提液单组分,而单一化学成分百秋里醇未见促胃肠动力作用。关键词：肢体缺血—再灌注（LIR）;胃排空;小肠推进
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- 416
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发布时间：2024-09-23 - 摘要：Objective: To investigate the anti-angiogenic mechanism of QHF (a formula of Chinese medicine ingredients, abbreviation Q for Qingrejiedu, H for Huoxuehuayu and F for Fuzhengguben) combined with small dose chemotherapy drug cisplatin(DDP)in H22 mice of hepatocellular carcinoma. Method: The forty eight Balb/c mice were inoculated with cell suspension of mouse hepatocellular carcinoma cell line H22 under the right axillary skin to establish the solid tumor model. Then they were randomly divided into four groups as follows: QHF group, DDP group, QHF+DDP group and NS (normal saline) group. The inhibition rates of tumor growth in tumor-bearing mice by drugs as an indicator were observed. The expression of VEGF, EGFR and MMP-2 in the tissue of mice tumor was detected with immunohistochemisty. Result: The weight of tumor was significantly lower in QHF, DDP and QHF+DDP group than that of in NS group(P<0.01), while the weight of mice tumor was significantly lower in QHF+DDP group than that of in QHF group. Their inhibition rate was 47.45%, 63.11%, 72.95%,respectively. The expression of VEGF and MMP-2 in the tissue of mice tumor was significantly lower in QHF, DDP and QHF+DDP group than that of in NS group(P<0.05, P<0.01 and P<0.01). Moreover, The expression of EGFR in the tissue of mice tumor was significantly lower in QHF, DDP and QHF+DDP group than that of in NS group(P<0.01, P<0.05 and P<0.01). However, the difference between QHF+DDP group and other groups has no significance(P>0.05). Conclusion: Both of QHF and small dose DDP have the anti-tumor and anti-angiogenic effect in H22 mice of hepatocellular carcinoma. Moreover, one of anti-angiogenic mechanism may be concerned with the downregulation of the expression of VEGF, EGFR and MMP-2 in the tissue of mice tumor.关键词：active ingredients;hepatic cell cancer;cisplatin;angiogenesis;mechanism
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发布时间：2024-09-23 - 摘要：Objective: To investigate the action of Wuling Capsules prescription ingredients and herbal withdrawal prescription and their effects on function of the original prescription. Method: The mouse model of hepatic fibrosis was established by multiple subcutaneous injection of carbon tetrachloride drinking 10% alcohol water solution,feeding hyperliposis-low protein diet and treated with experiment drugs for 10 days. The activities of ALT and CHE in serum of mice were detected by enzyme method. HSC-T6 cell was activated by transforming growth factor-beta1 in vitro,effects of the prescription ingredients and withdrawal drug compatibility on the expression of Ras /ERK and TGF-β /Smad signaling pathway pretein in HSC-T6 cell were observed. The expression of α-SMA, Ras,CollagenⅠ,ERK,p-ERK,TβRⅠ,TβRⅡ,Smad2 /3 were determined by western bloting. Result:The activity of ALT in surum of hepatic fibrisis mice was decreased by WuLing Capsules,prescription ingredients and withdrawal drug compatibility groups,while Bupleurum chinensis DC and Ganoderma group had no effects. WuLing Capsules, prescription ingrediets and withdrawal drug group had different biological effect on the protein expression of α-SMA, Collagen,ERK,p-ERK,TβRⅠ,TβRⅡ,Smad2 /3 in HSC-T6 cell induced by TGF-β1. Conclusion:Prescription ingredients compatibility produces additive effects on WuLing Capsules therapy for hepaticfibrosis mice,they inhibit the protein expression of ERK and TGF-β1 /Smad signaling pathway in HSC-T6 by TGF-β1 activation,subsequently influence the transcription and synthesis of collagenⅠin HSC-T6.关键词：Wuling Capsules;herbal withdrawal analysis;prescription ingredient compatibility;hepatic stellate cell
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- 164
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- 4
发布时间：2024-09-23 - 摘要：目的:采用血清药理学的方法,观察小半夏加茯苓颗粒对肝癌SMMC-7721细胞的抑制作用。方法:设空白对照组、环磷酰胺（CTX）组、原方汤剂组及颗粒剂不同剂量组,分别制备成含药血清与体外培养的肝癌SMMC-7721细胞共孵,倒置显微镜观察各组SMMC-7721细胞生长形态变化以及用MTT法检测其细胞增殖抑制率。结果:与空白对照组比较,CTX组、原方汤剂组以及颗粒不同剂量组均使SMMC-7721细胞生长形态发生明显改变,细胞生长不良,细胞数量减少;MTT实验结果显示CTX组、汤剂组、颗粒不同剂量组对SMMC-7721细胞作用24h、48h、72h后,细胞增殖抑制率逐渐升高（P<0.05）。各时段内各中药组对细胞的抑制率均低于CTX组（P<0.05）,原方汤剂组与颗粒大剂量组无显著差异（P>0.05）。结论:小半夏加茯苓颗粒含药血清对体外培养的肝癌SMMC-7721细胞具有明显的抑制作用,且其作用呈现时间-效应关系和剂量-效应关系。关键词：小半夏加茯苓颗粒;含药血清;肝癌SMMC-7721;噻唑蓝
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发布时间：2024-09-23 - 摘要：Objective: To investigate the action of Wuling Capsules prescription ingredients and herbal withdrawal prescription and their effects on function of the original prescription. Method: The mouse model of hepatic fibrosis was established by multiple subcutaneous injection of carbon tetrachloride drinking 10% alcohol water solution, feeding hyperliposis-low protein diet and treated with experiment drugs for 10 days. The activities of ALT and CHE in serum of mice were detected by enzyme method. HSC-T6 cell was activated by transforming growth factor-beta1 in vitro,effects of the prescription ingredients and withdrawal drug compatibility on the expression of Ras/ERK and TGF-β/Smad signaling pathway pretein in HSC-T6 cell were observed. The expression of α-SMA,Ras,CollagenⅠ,ERK,p-ERK,TβRⅠ,TβRⅡ,Smad2/3 were determined by western bloting. Result: The activity of ALT in surum of hepatic fibrisis mice was decreased by WuLing Capsules, prescription ingredients and withdrawal drug compatibility groups, while Bupleurum chinensis DC and Ganoderma group had no effects. WuLing Capsules,prescription ingrediets and withdrawal drug group had different biological effect on the protein expression of α-SMA, Collagen, ERK, p-ERK, TβRⅠ,TβRⅡ, Smad2/3 in HSC-T6 cell induced by TGF-β1. Conclusion: Prescription ingredients compatibility produces additive effects on WuLing Capsules therapy for hepaticfibrosis mice, they inhibit the protein expression of ERK and TGF-β1/Smad signaling pathway in HSC-T6 by TGF-β1 activation, subsequently influence the transcription and synthesis of collagenⅠin HSC-T6.关键词：Wuling Capsules;herbal withdrawal analysis;prescription ingredient compatibility;hepatic stellate cell
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发布时间：2024-09-23 - 摘要：Objective: To observe the effects of You Lv kang on depression behavior,plasma cyclic adenosine monophosphate（ cAMP）,cyclic guanosine monophosphate （ cGMP）,and cell morphology changes of plantar sweat gland cells in the model rat of kidey-Yin deficiency and depression syndrome. Method: Rats were divided into seven groups randomly. The rat model of kidney-Yin deficiency was established by exposing the animals to chronic unpredicable stress and giving thyroxine by intragastric adminstration. The model establishment and the treatment by different drugs were started at the same time. The dose for fluoxetine group was 3. 6 mg·kg -1,the dose of Liuwei Dihuang Wan group was 1. 6 g·kg -1. The three doses of You Lv Kang were 7. 2 g·kg -1,14. 4 g·kg -1 and 28. 8 g·kg -1 respectively. The blank and model groups were adminstrated with water daily. All the treatments lasted for 21 days. The behavior changs was evaluated by the open-field test and fluid comsumption test. The plasma samples of the rats were taken for cAMP and cGMP determination by radioimmunoassay. The morphology changes of plantar sweat gland cells with HE staining in rats were investigated under light microscope. Result:All the indexes of the open-field test and fluid comsumption test in the model rats were significantly decreased,with significantly increased the content of plasma cAMP. Vacuole-like changes in plantar sweat gland cells in the model rats were obvious,and sweat gland ducts were expanded. You Lv Kang could improve the behavior of the rats. The level of cAMP was significantly decreased in the Liuwei Dihuang Wan and You Lv Kang groups,and the morphological appearance of plantar sweat gland cells showed to be basically normal,especially in the high dose group of You Lv Kang and Liuwei Dihuang Wan group. Conclusion: You Lv Kang can regulate β-receptor system and improve the pathological changes of plantar sweat gland cell. Nourishing Yin might be one of the mechanisms responsible for the effects.关键词：depression;kidney-Yin deficiency;You Lv Kang;cAMP;cell morphology;rat
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- 211
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发布时间：2024-09-23 - 摘要：Objective:To observe effects of Shenchang Injection on platelet activation and thrombosis in MCAO rats. Method: The cerebral injury model induced by ischemia /reperfusion was made in rat by MCAO. Shenchang injection was given by iv for 7 times,then CD62p、CD63 were measured by flow-cytometry. Thrombosis and clotting time were observed. Result:Shenchang injection could decrease expression of CD62p and CD63 in blood,decline thrombosis in kidney vein,delay clotting time in MCAO rats. Conclusion:Shenchang injection is effect for antiplatelet activities and postponement of thrombosis by extending blood clotting. These are likely the pharmacological basis of the injection for treating stroke.关键词：Shenchang Injection;Acorus tatarinowii Schott;borneol;MCAO;platelet activation
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- 142
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发布时间：2024-09-23 - 摘要：Objective: To observe the effects of You Lv kang on depression behavior, plasma cyclic adenosine monophosphate(cAMP), cyclic guanosine monophosphate(cGMP), and cell morphology changes of plantar sweat gland cells in the model rat of kidey-Yin deficiency and depression syndrome. Method: Rats were divided into seven groups randomly. The rat model of kidney-Yin deficiency was established by exposing the animals to chronic unpredicable stress and giving thyroxine by intragastric adminstration. The model establishment and the treatment by different drugs were started at the same time. The dose for fluoxetine group was 3.6 mg ·kg-1, the dose of Liuwei Dihuang Wan group was 1.6 g ·kg-1. The three doses of You Lv Kang were 7.2 g ·kg-1, 14.4 g ·kg-1 and 28.8 g ·kg-1 respectively. The blank and model groups were adminstrated with water daily. All the treatments lasted for 21 days.The behavior changs was evaluated by the open-field test and fluid comsumption test. The plasma samples of the rats were taken for cAMP and cGMP determination by radioimmunoassay. The morphology changes of plantar sweat gland cells with HE staining in rats were investigated under light microscope. Result: All the indexes of the open-field test and fluid comsumption test in the model rats were significantly decreased, with significantly increased the content of plasma cAMP. Vacuole-like changes in plantar sweat gland cells in the model rats were obvious, and sweat gland ducts were expanded. You Lv Kang could improve the behavior of the rats. The level of cAMP was significantly decreased in the Liuwei Dihuang Wan and You Lv Kang groups, and the morphological appearance of plantar sweat gland cells showed to be basically normal, especially in the high dose group of You Lv Kang and Liuwei Dihuang Wan group. Conclusion: You Lv Kang can regulate β-receptor system and improve the pathological changes of plantar sweat gland cell. Nourishing Yin might be one of the mechanisms responsible for the effects.关键词：depression;kidney-Yin deficiency;You Lv Kang;cAMP;cell morphology;rat
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发布时间：2024-09-23 - 摘要：Objective:To investigate the influences of Aβ25-35 on the TLR3 and TLR4 mRNA. Method: NG108-15 cell line was stimulated with Aβ25-35,the supernatant was collected 24 hours later and the inflammatory factors TNF-α and IFN-β were quantitatively measured,the changes in the mRNA expression of TLR3 and TLR4 were detected by real-time PCR. Result:Aβ induced the over-secreting of TNF-α、IFN-β and over-expression of TLR3 and TLR4 mRNA （P < 0. 01）. Conclusions:Aβ induced the over-secreting of inflammatory factors and over-expression of TLR3 and TLR4,indicating the action of Aβ in causing AD disease might be affiliated with the immune inflammation and the TLRs signaling pathway.关键词：β-Amyloid;NG108-15;Toll-like receptors;TNF-α;IFN-β
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- 201
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发布时间：2024-09-23 - 摘要：Objective: To observe effects of Shenchang Injection on platelet activation and thrombosis in MCAO rats. Method: The cerebral injury model induced by ischemia/reperfusion was made in rat by MCAO. Shenchang injection was given by iv for 7 times, then CD62p、CD63 were measured by flow-cytometry. Thrombosis and clotting time were observed. Result: Shenchang injection could decrease expression of CD62p and CD63 in blood, decline thrombosis in kidney vein, delay clotting time in MCAO rats. Conclusion: Shenchang injection is effect for antiplatelet activities and postponement of thrombosis by extending blood clotting. These are likely the pharmacological basis of the injection for treating stroke.关键词：Shenchang Injection;Acorus tatarinowii Schott;borneol;MCAO;platelet activation
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the effect of the polysaccharide from Eclipta prostrasta L. on the immune function of mice. Method:Kunming mice were randomly divided into 5 groups,the low,middle,high doses of the polysaccharide,the positive drug group and normal control group,treated ig for 14 days. All the mice were experimented with peritoneal macrophage,hemolysin and hemolytic antibody plaques formation. Result:Compared with normal,polysaccharide of Eclipta prostrasta L. of three doses significantly increased the peritoneal macrophages phagocytic index and percentage（P < 0. 01）,significantly promoted the hemolysin and hemolytic plaque formation in normal mice,particularly at the low dosage. Conclusion: The polysaccharide of Eclipta prostrasta L. can significantly increase the immune function in normal mice.关键词：polysaccharides of Eclipta prostrasta L.;immune activity;mice
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- 442
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发布时间：2024-09-23 - 摘要：Objective: To investigate the influences of Aβ25-35 on the TLR3 and TLR4 mRNA. Method: NG108-15 cell line was stimulated with Aβ25-35, the supernatant was collected 24 hours later and the inflammatory factors TNF-α and IFN-β were quantitatively measured, the changes in the mRNA expression of TLR3 and TLR4 were detected by real-time PCR. Result: Aβ induced the over-secreting of TNF-α、IFN-β and over-expression of TLR3 and TLR4 mRNA (P<0.01). Conclusions: Aβ induced the over-secreting of inflammatory factors and over-expression of TLR3 and TLR4, indicating the action of Aβ in causing AD disease might be affiliated with the immune inflammation and the TLRs signaling pathway.关键词：β-Amyloid;NG108-15;toll-like receptors;TNF-α;IFN-β
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Puwei Pill（PWP） on the anti-oxidation and improvement of the gastric mucosal blood flow in the rats with gastric ulcer recurrence. Method:The gastric ulcer recurrence model was developed by intraperitoneal injection with interleukin-1β （ IL-1β ）. Then the pathohistological structure in the recurred gastric ulcer,treated by PWP （ ig,90 d）,was observed. The superoxide dismutase （ SOD）,nitric oxide （NO）,malondialdehyde（MDA） and endothelin-1 （ET-1） in the plasma and ET-1A expressed in the gastric mucosa were determined. Result: PWP could significantly resist the recurrence of gastric ulcer,increase the SOD,NO activity and decrease the contents of MDA and ET-1 in plasma. At the same time,PWP could significantly inhibit the ET-1A expression in the gastric mucosa. Conclusion:PWP can prevent the recurrence of the gastric ulcer, which mechanism is probably related to the increase of anti-oxidation capacity and improvement of mucosal blood flow.关键词：gastric ulcer;recurrence;Puwei Pill;anti-oxidation;blood flow
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- 198
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发布时间：2024-09-23 - 摘要：Objective: To evaluate the effect of the polysaccharide from Eclipta prostrasta L. on the immune function of mice. Method: Kunming mice were randomly divided into 5 groups,the low,middle,high doses of the polysaccharide, the positive drug group and normal control group,treated ig for 14 days. All the mice were experimented with peritoneal macrophage,hemolysin and hemolytic antibody plaques formation. Result: Compared with normal, polysaccharide of Eclipta prostrasta L. of three doses significantly increased the peritoneal macrophages phagocytic index and percentage(P<0.01),significantly promoted the hemolysin and hemolytic plaque formation in normal mice,particularly at the low dosage. Conclusion: The polysaccharide of Eclipta prostrasta L. can significantly increase the immune function in normal mice.关键词：polysaccharides of Eclipta prostrasta L.;immune activity;mice
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发布时间：2024-09-23 - 摘要：Objective:To observe anti-inflammatory and analgesic effects of Jiangujuanbi Capsule. Method: Rat adjuvant arthritis model was established by Freund’s complete adjuvant to observe the joint inflammation;mice ear swelling by croton oil was also used. To evaluate the anti-inflamatory action of Jiangujuanbi Capsule,cotton ball granuloma in rats was used. Writhing method and hot plate method were used to observe the Jiangujuanbi Capsule’s analgesic effect in mice. Result: Jiangujuanbi Capsule could inhibit the rat arthritis,significantly reduce mice ear swelling caused by croton oil,and could reduce granuloma of rats. Jiangujuanbi Capsule reduce obviously the pain of mice which caused by acetic acid and hot plate. Conclusion: Jiangujuanbi Capsule has a good anti-inflammatory and analgesic effects.关键词：Jiangujuanbi Capsule;anti-inflammation;analgesia
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- 102
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of Puwei Pill(PWP) on the anti-oxidation and improvement of the gastric mucosal blood flow in the rats with gastric ulcer recurrence. Method: The gastric ulcer recurrence model was developed by intraperitoneal injection with interleukin-1β(IL-1β). Then the pathohistological structure in the recurred gastric ulcer,treated by PWP(ig,90 d), was observed. The superoxide dismutase(SOD),nitric oxide(NO),malondialdehyde(MDA) and endothelin-1 (ET-1) in the plasma and ET-1A expressed in the gastric mucosa were determined. Result: PWP could significantly resist the recurrence of gastric ulcer, increase the SOD,NO activity and decrease the contents of MDA and ET-1 in plasma. At the same time, PWP could significantly inhibit the ET-1A expression in the gastric mucosa. Conclusion: PWP can prevent the recurrence of the gastric ulcer, which mechanism is probably related to the increase of anti-oxidation capacity and improvement of mucosal blood flow.关键词：gastric ulcer;recurrence;Puwei Pill;anti-oxidation;blood flow
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发布时间：2024-09-23 - 摘要：目的:研究逍遥散对D-gal所致老年性痴呆（AD）模型小鼠学习记忆能力及中枢胆碱能神经元活性的影响,探讨逍遥散防治AD的作用及其机理。方法:采用igD-gal复制AD小鼠模型,同时用逍遥散低、中、高剂量组进行对抗治疗,共42天。第41天进行学习能力训练,第42天进行记忆能力测试,然后取脑测定脑组织乙酰胆碱脂酶（AchE）、乙酰胆碱转移酶（ChAT）活性。结果:逍遥散低、中、高剂量组均可提高小鼠学习记忆能力,降低潜伏时间,但以高剂量组最为明显;逍遥散低、中、高剂量组皆可降低脑组织AchE活性,提高ChAT活性,但亦以高剂量组最为明显。结论:逍遥散能较好的对抗D-gal所致AD小鼠模型,其机制可能与调节中枢胆碱神经递质活性和数量有关。关键词：逍遥散;老年性痴呆;学习记忆能力;潜伏时间;乙酰胆碱酯酶;胆碱乙酰转移酶
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- 624
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发布时间：2024-09-23 - 摘要：Objective: To observe anti-inflammatory and analgesic effects of Jiangujuanbi Capsule. Method: Rat adjuvant arthritis model was established by Freunds complete adjuvant to observe the joint inflammation;mice ear swelling by croton oil was also used.To evaluate the anti-inflamatory action of Jiangujuanbi Capsule, cotton ball granuloma in rats was used.Writhing method and hot plate method were used to observe the Jiangujuanbi Capsules analgesic effect in mice. Result : Jiangujuanbi Capsule could inhibit the rat arthritis,significantly reduce mice ear swelling caused by croton oil,and could reduce granuloma of rats.Jiangujuanbi Capsule reduce obviously the pain of mice which caused by acetic acid and hot plate.Conclusion: Jiangujuanbi Capsule has a good anti-inflammatory and analgesic effects.关键词：Jiangujuanbi Capsule;anti-inflammation;analgesia
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发布时间：2024-09-23 - 摘要：目的:观察中药一贯煎对肿瘤坏死因子-α（TNF-α）致肝炎小鼠模型的干预作用。方法:24只雄性C57BL/6小鼠随机分为4组:正常组、模型组每日每只小鼠ig2mL生理盐水;中药组给予一贯煎汤药ig,12.6g·kg-1,1次/d,连续1周;阳性对照组给予联苯双酯滴丸ig,7.5×10-3g·kg-1,1次/d,连续1周。实验第8天晚10时给予模型组、中药组、阳性对照组每只小鼠0.1μgTNF/12mgGaINip,进行肝损伤造模,观察小鼠状态,于次日上午9时处死所有小鼠,测定血清丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）;HE染色观察肝组织病理变化;WesternBlot方法对肝组织cIAP1蛋白检测。结果:一贯煎组小鼠血清ALT、AST较模型组显著降低,小鼠肝组织病理改变较模型组显著减轻,cIAP1蛋白表达较模型组显著增高,说明一贯煎能够促进肝组织cIAP1蛋白的表达。结论:一贯煎可能通过调节TNF-α信号通路cIAP1蛋白的表达,从而有效减轻炎症反应,达到保肝、降酶的目的。关键词：一贯煎;肝功能;凋亡抑制;蛋白1
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- 391
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发布时间：2024-09-23 - 摘要：Objective:To investigate the the protective effects of Epigallocatechin-3-gallate（EGCG） on the release of inflammatory cytokines and expression of neurotrophic factors in spinal cord-injuried（SCI） rats. Method: SCI model was reproduced by hemisection,and EGCG was administered for 24 h after hemisection 5 min after SCI. The concentrations of serum IL-6 and IL-8 were measured by ELISA assay,and the expression of neurotrophin-3 （NT-3） and brain-derived neurotrophic factor（BDNF） protein in injured spinal cord tissues were determined by western blot. Result: EGCG significantly reduced the levels of serum IL-6 and IL-8,and increased expression of NT-3 and BDNF at the injured spinal cord in SCI rats. Conclusion: EGCG may protect against spinal cord from injury by inhibiting release of inflammatory cytokines and enhancing the expression of endogenous neurotrophic factors in SCI rats.关键词：spinal cord injury（SCI）;epigallocatechin-3-gallate（EGCG）;IL-6;IL-8;NT-3;BDNF
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- 268
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发布时间：2024-09-23 - 摘要：目的:建立益肾健骨丸等9种医院中药制剂微生物限度检查法。方法:测定9种医院中药制剂对5种验证菌株的回收率,并对控制菌的检查法进行验证。结果:健胃消胀合剂、健胃止痛合剂、补肺益寿合剂I号和补肺益寿合剂Ⅱ号4种供试品试验菌的回收率试验均高于70%,证明无抑菌现象;制萎扶胃丸和运脾颗粒有一定程度的抑菌作用,但可通过稀释法消除抑菌作用;益肾健骨丸、杜仲腰痛丸和祛风颗粒的抑菌作用强,必须采用薄膜过滤法才能彻底消除其抑菌作用。结论:健胃消胀合剂、健胃止痛合剂、补肺益寿合剂Ⅰ号和补肺益寿合剂Ⅱ号可按常规方法进行微生物限度检查;制萎扶胃丸和运脾颗粒按培养基稀释法进行微生物限度检查;益肾健骨丸、杜仲腰痛丸和祛风颗粒则按薄膜过滤法进行徽生物限度检查。关键词：医院中药制剂;微生物限度检查法;方法验证
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- 158
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发布时间：2024-09-23 - 摘要：Objective: To investigate the the protective effects of Epigallocatechin-3-gallate(EGCG) on the release of inflammatory cytokines and expression of neurotrophic factors in spinal cord-injuried(SCI) rats. Method: SCI model was reproduced by hemisection, and EGCG was administered for 24 h after hemisection 5 min after SCI. The concentrations of serum IL-6 and IL-8 were measured by ELISA assay, and the expression of neurotrophin-3(NT-3)and brain-derived neurotrophic factor(BDNF)protein in injured spinal cord tissues were determined by western blot. Result : EGCG significantly reduced the levels of serum IL-6 and IL-8, and increased expression of NT-3 and BDNF at the injured spinal cord in SCI rats. Conclusion : EGCG may protect against spinal cord from injury by inhibiting release of inflammatory cytokines and enhancing the expression of endogenous neurotrophic factors in SCI rats.关键词：spinal cord injury(SCI);epigallocatechin-3-gallate(EGCG);IL-6;IL-8;NT-3;BDNF
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发布时间：2024-09-23 - 摘要：目的:观察扶正平喘颗粒的平喘作用。方法:采用卵清蛋白致敏喷雾法观察平喘作用。结果:扶正平喘颗粒能明显延长哮喘小鼠的引喘潜伏期,减少外周血嗜酸性粒（EOS）细胞的计数及肺组织组胺的释放。结论:扶正平喘颗粒具有明显的平喘作用且能降低哮喘小鼠外周血EOS细胞数及明显抑制肺组织组胺的释放。关键词：扶正平喘颗粒;哮喘;药效评价
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- 185
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发布时间：2024-09-23 - 摘要：<正>依据文献计量学的原理和方法,经研究人员对相关文献的检索、计算和分析,以及学科专家评审,《中国实验方剂学杂志》入编《中文核心期刊要目总览》2008年版（即第五版）之中国医学类的核心期刊。该核心期刊按《中国图书馆分类法》的学科体系,列出了73个学科的核心期刊表,并逐一对核心期刊进行了著录。著录项目包括:题名、并列题名、主办单位、创刊时间、出版周期、学科分类号、ISSN号、CN号、邮发代号、编辑部
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发布时间：2024-09-23 - 摘要：目的:探讨活血补肾汤对椎动脉型颈椎病（cervical spondylosis of vertebral artery type,CSA）心率变异性的影响及颈交感神经对CSA的影响。方法:CSA患者（肾虚血瘀型）49例,随机分为试验组和对照组,分别予以活血补肾汤和颈舒颗粒,观测治疗前、后患者症状、体征、眩晕积分并测定心率变异性（heart rate variability,HRV）相关指标:心率全部窦性心率间期的标准差（standard diviation of NN intervals,SDNN）、低频（low frequencies,LF）、低频/高频（LF/HF）。结果:治疗后SDNN明显升高,LF、LF/HF显著降低（P<0.05）;同时患者症状体征及眩晕改善程度与SDNN、LF、LF/HF有明显的相关性（r=0.545）,而且眩晕程度与SDNN、LF有显著依存性（P<0.05）。结论:CSA患者存在HRV降低现象,活血补肾汤可能通过改善HRV,降低交感神经张力而发挥临床效应;颈交感神经张力增高是CSA的发病原因之一。关键词：椎动脉型颈椎病;心率变异性;交感神经;活血补肾
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- 157
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发布时间：2024-09-23 - 摘要：目的:观察安神定志丸治疗抑郁症心胆气虚型的临床疗效。方法:将60例抑郁症心胆气虚型患者采用随机数字表法分为治疗组30例,给予安神定志汤剂;对照组30例给予氟西汀治疗,疗程为6周。在治疗1,2,4,6周时,对两组的汉密顿（HAMD）减分疗效、中医证候疗效进行对比观察。结果:两组在治疗1周时的HAMD减分率,其差异具有统计学意义（P<0.01）,而在治疗2,4,6周时HAMD减分率差异无统计学意义;两组间中医证候疗效在各时间点比较,其差异具有统计学意义（P<0.01）,安神定志汤剂对抑郁症心胆气虚型中医证候疗效明显优于氟西汀。结论:安神定志汤剂治疗抑郁症心胆气虚型具有良好的临床疗效。关键词：安神定志汤剂;抑郁症;心胆气虚;氟西汀
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发布时间：2024-09-23 - 摘要：目的:探讨早期抗抑郁治疗对脑血管病预后的影响。方法:将60例早期抑郁患者随机分为治疗组30例,对照组30例。治疗组早期给予抗抑郁治疗,对照组除不用抗抑郁治疗之外其他治疗同治疗组。共同观察4周。结果:观察组运动功能评测（MAS）及Barthel指数评分与对照组比较有明显提高（P<0.01）,其住院时间及治疗费用两组比较差异无显著性意义。结论:脑卒中后抑郁患者行早期心理干预和药物治疗能明显提高康复疗效。关键词：抑郁症;脑血管;康复治疗
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- 81
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发布时间：2024-09-23 - 摘要：Objective:To observe and study Isotretinoin soft capsules （TaiErSi） combined with Qing-gan Qu-cuo recipe in treating cyst jialing river acne. Method:50 patients cyst jialing river acne patients were randomly divided into two groups,Isotretinoin soft capsules （TaiErSi） 20mg,2 times every day. The treatment group （ n = 30） was added with qing-gan qu-cuo recipe daily. In the treatment of initial and 2,4,8 weeks,observing and studying the lesions’ recovery,assessing skin evaluation for 16 weeks and adverse reaction of recurrence. Result: The efficiency of treatment group and control group was 90. 0% vs 45. 0% . Total skin lesions means,inflammatory lesions means and noninflammatory lesions means of treatment group 8 weeks after treatment with before treatment, were statistically significant difference （P < 0. 05 or P < 0. 01）. The control group’s total skin lesions means and inflammatory skin lesions means of 8 weeks after treatment compared with before treatment were statistically significant differente （P < 0. 05 or P < 0. 01）,the noninflammatory lesions means of control group before and after treatment were no significant differente （ P > 0. 05 ）. 8 weeks after treatment,total skin lesions means and noninflammatory skin lesions means of treatment group are better than those of control group significantly（P < 0. 05 or P < 0. 01）,the inflammatory lesions means of both groups 8 weeks after treatment were no statistically significant difference between the two groups （P > 0. 05）. Conclusion:Isotretinoin soft capsules combined with qing-gan qucuo recipe in treating Cyst jialing river acne has reliable effect,low recurrent rate and less adverse reactions.关键词：Isotretinoin soft capsules;qing-gan qu-cuo recipe;Cyst jialing river acne;lesions
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- 157
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发布时间：2024-09-23 - 摘要：Objective: To observe and study Isotretinoin soft capsules (TaiErSi) combined with Qing-gan Qu-cuo recipe in treating cyst jialing river acne. Method: 50 patients cyst jialing river acne patients were randomly divided into two groups, Isotretinoin soft capsules (TaiErSi) 20mg, 2 times every day. The treatment group (n=30) was added with qing-gan qu-cuo recipe daily.In the treatment of initial and 2, 4, 8 weeks,observing and studying the lesions recovery, assessing skin evaluation for 16 weeks and adverse reaction of recurrence. Result: The efficiency of treatment group and control group was 90.0% vs 45.0%. Total skin lesions means, inflammatory lesions means and noninflammatory lesions means of treatment group 8 weeks after treatment with before treatment, were statistically significant difference (P<0.05 or P<0.01). The control groups total skin lesions means and inflammatory skin lesions means of 8 weeks after treatment compared with before treatment were statistically significant differente (P<0.05 or P<0.01),the noninflammatory lesions means of control group before and after treatment were no significant differente (P>0.05). 8 weeks after treatment, total skin lesions means and noninflammatory skin lesions means of treatment group are better than those of control group significantly(P<0.05 or P<0.01),the inflammatory lesions means of both groups 8 weeks after treatment were no statistically significant difference between the two groups (P>0.05). Conclusion: Isotretinoin soft capsules combined with qing-gan qu-cuo recipe in treating Cyst jialing river acne has reliable effect, low recurrent rate and less adverse reactions.关键词：Isotretinoin soft capsules;qing-gan qu-cuo recipe;Cyst jialing river acne;lesions
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发布时间：2024-09-23 - 摘要：Objective:To explore the application of association rules in sleep problems of menopause. Method:Association rules analysis was applied to menopause data processing through setting minimum support and minimum confidence,using Apriori algorithm in the process. Result:23 association rules were discovered between sleep and somatization. symptoms. There were 10 two-way association rules,2 association rules,1 non-association rule. Conclusion:Rules of Sleep problems and Somatic symptoms can be discovered by employing association rules in analyzing menopause cases. And these rules provide im portant reference for prevention and cure of sleep issue.关键词：menopause;sleep problems;association rules
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发布时间：2024-09-23 - 摘要：Objective: To explore the application of association rules in sleep problems of menopause. Method: Association rules analysis was applied to menopause data processing through setting minimum support and minimum confidence,using Apriori algorithm in the process. Result: 23 association rules were discovered between sleep and somatization.symptoms. There were 10 two-way association rules,2 association rules,1 non-association rule. Conclusion: Rules of Sleep problems and Somatic symptoms can be discovered by employing association rules in analyzing menopause cases.And these rules provide im portant reference for prevention and cure of sleep issue.关键词：menopause;sleep problems;association rules
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of Shenmai injection on perioperation T-lymphocyte subsets and natural killer cells for patients received cervical cancer. Method: 68 patients with cervical cancer were randomly divided into two groups,34 patients were in each group. Shenmai group was received 50 mL Shenmai injection diluted with 300 mL 5% glucose 1 day before operation until 7 day after operation,and control group was received only 300 mL 5% glucose. Peripheral venous blood samples were taken before operation（ T1）,the end of operation（T2） and the seventh day after operation（T3） for determination of CD3 + 、CD4 + 、CD8 + 、CD4 + /CD8 + and NK. Result: The CD3 + 、CD4 + 、CD8 + 、CD4 + /CD8 + and NK were decreased in both groups at the end of operation （P < 0. 05 or P < 0. 01）. On the seventh postoperation day,the CD3 + 、CD4 + 、CD8 + 、CD4 + /CD8 + and NK were returned to preoperational levels in both groups,but there was a significant change between the two groups（ P < 0. 01）. Conclusion: Shenmai injection can improve the inhibition effects because of operation,and promote to recover the immunologic function for patients received cervical cancer.关键词：Shenmai injection;Cellular Immunologic Function;Cervical Cancer
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the effectiveness of ShuYu Capsule in patients with vascular depresion （VD）. Method: The investigational drugs were packed by blind method. A randomized,double-blind and controlled trial was conducted on sixty patients with VD. The sixty patients were randomly divided into treatment group （n = 30）,orally ShuYu Capsule 3# tid and Fluoxetine capsule placebo 20mg qd,for 8 wk. and control group （n = 30）,orally Fluoxetine capsule 20mg qd,and ShuYu Capsule placebo 3 # tid,for 8 wk. All subjects were assessed by HAMD,MMSE,CGI,NDS and Blood lipids. Result: ShuYu Capsule can be reduced the scores of HAMD,MMSE,CGI of Before and after treatment,The difference has statistics meaning （ P < 0. 05） between two groups; Can reduce the NDS grading and blood LDL-C level （ P < 0. 05） after 8 w in treatment group,But NDS grade and the blood LDL-C level can not be improved obviously （ P > 0. 05） in control group. Have not statistics significantly （ P > 0. 05 ） in the always efficient comparative difference between two groups of treatment. Conclusion: ShuYu Capsule has better clinical effect in vascular depresion,equal effect to Fluoxetine capsule.关键词：herbal preparations;vascular depresion;randomized controlled trial;double-blinded method;traditional Chinese medicine therapy
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- 178
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发布时间：2024-09-23 - 摘要：Objective: To evaluate the effectiveness of ShuYu Capsule in patients with vascular depresion (VD). Method: The investigational drugs were packed by blind method. A randomized, double-blind and controlled trial was conducted on sixty patients with VD. The sixty patients were randomly divided into treatment group (n=30),orally ShuYu Capsule 3# tid and Fluoxetine capsule placebo 20mg qd,for 8 wk.and control group (n=30), orally Fluoxetine capsule 20mg qd,and ShuYu Capsule placebo 3# tid,for 8 wk. All subjects were assessed by HAMD,MMSE,CGI, NDS and Blood lipids. Result: ShuYu Capsule can be reduced the scores of HAMD,MMSE,CGI of Before and after treatment,The difference has statistics meaning (P<0.05) between two groups; Can reduce the NDS grading and blood LDL-C level (P<0.05) after 8 w in treatment group,But NDS grade and the blood LDL-C level can not be improved obviously (P>0.05) in control group. Have not statistics significantly (P>0.05) in the always efficient comparative difference between two groups of treatment. Conclusion: ShuYu Capsule has better clinical effect in vascular depresion,equal effect to Fluoxetine capsule.关键词：herbal preparations;vascular depresion;randomized controlled trial;double-blinded method;traditional Chinese medicine therapy
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发布时间：2024-09-23 - 摘要：Objective: To observe the curative effect of Tiaozhi Mistura in treating hyperlipemia and improving hemorhelogy. Method:To patients with hyperlipemia were chosen,and diveded into treated group and control group The treated group was treated with Tiaozhi Mistura and the control group with Xuezhikang The course of treatment was five weeks. Blood fat and hemorheology of the twe groups were observed Result:The improvement of TC、TG、LDL-C、HDL-C hemoviscosity,plasmoviscosity and hematocrit of the treated group Was more significant than those in the control（ P < 0. 05）. Conclusion:Tiaozhi Mistura has good curative effect on hyperlipemia and improving of hemorheology.关键词：Mistura;hyperlipemia;hemorheology
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- 125
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发布时间：2024-09-23 - 摘要：Objective: To observe the curative effect of Tiaozhi Mistura in treating hyperlipemia and improving hemorhelogy. Method :To patients with hyperlipemia were chosen, and diveded into treated group and control group The treated group was treated with Tiaozhi Mistura and the control group with Xuezhikang The course of treatment was five weeks. Blood fat and hemorheology of the twe groups were observed Result :The improvement of TC、TG、LDL-C、HDL-C hemoviscosity,plasmoviscosity and hematocrit of the treated group Was more significant than those in the control(P<0.05). Conclusion :Tiaozhi Mistura has good curative effect on hyperlipemia and improving of hemorheology.关键词：Mistura;hyperlipemia;hemorheology
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发布时间：2024-09-23 - 摘要：Objective: In this paper, the femoral neck bone mineral density(BMD) data in patients with type 2 diabetes compared with those of the normal database are analysed in order to explore the influences of type 2 diabetes on bone mineral density in men and women with different ages. Method : The dual-energy X-ray absorptiometry (DEXA) is uesd to obtain the femoral neck BMD data in patients with type 2 diabetes by gender and age, then the means and standard deviations of BMD, low bone mass detection rates, and osteoporosis detection rates were calculated, and the results were displayed and analyzed. Result : The male and female patients with type 2 diabetes compared with normal bone neck BMD reduced in varying degrees. Conclusion : Type 2 diabetes affected the levels of BMD to varying degrees,and there were significant gender differences in low bone mass and osteoporosis incidence rates.关键词：type 2 diabetes;femoral neck;bone mineral density
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发布时间：2024-09-23 - 摘要：Objective: In this paper,the femoral neck bone mineral density（BMD） data in patients with type 2 diabetes compared with those of the normal database are analysed in order to explore the influences of type 2 diabetes on bone mineral density in men and women with different ages. Method: The dual-energy X-ray absorptiometry （DEXA） is uesd to obtain the femoral neck BMD data in patients with type 2 diabetes by gender and age,then the means and standard deviations of BMD,low bone mass detection rates,and osteoporosis detection rates were calculated,and the results were displayed and analyzed. Result: The male and female patients with type 2 diabetes compared with normal bone neck BMD reduced in varying degrees. Conclusion: Type 2 diabetes affected the levels of BMD to varying degrees,and there were significant gender differences in low bone mass and osteoporosis incidence rates.关键词：type 2 diabetes;femoral neck;bone mineral density
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发布时间：2024-09-23 - 摘要：目的:通过对中西医结合治疗单纯疱疹病毒性角膜炎的临床观察,评价其临床疗效及安全性。方法:随机选取符合研究标准的单纯疱疹病毒性角膜炎的病例,随机分为治疗组和对照组。治疗组应用中西医结合治疗,口服院内自制中药制剂菊兰清明片或谷龙清明片+西药。对照组单纯应用西药治疗。共连续治疗3周。结果:治疗组治愈率为76.7%,有效率为20%,总有效率为96.7%;对照组治愈率为61.7%,有效率为21.6%,总有效率为83.3%。经统计学处理,两组之间的治愈率和总有效率有显著性差异（P<0.05）。治疗组平均治愈时间短于对照组。治疗组明显优于对照组。治愈病例均随访1年,治疗组无复发:对照组复发10例,占对照组治愈病人的27%。结论:中西医结合治疗单纯疱疹病毒性角膜炎疗效优于单纯西医治疗,疗程短,见效快,减少复发。关键词：中西医结合;单纯疱疹病毒性角膜炎;菊兰清明片;谷龙清明片
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发布时间：2024-09-23 -
Analysis and Thought on Eighteen Incompatible Medicaments Based on the Clinical Usage in Modern Time
摘要：To explore the basic rules of clinical application about eighteen incompatible medicaments contained in prescriptions in modern time. 1964 formulas were screened out of TCM journal databases and studied based on the method of frequency analysis and maximal frequent item sets mining. The result showed that the prescriptions existed in all kinds of clinical trials,and were mainly used to treat the difficult and complicated cases such as cardio-cerebrovascular disease, cancer,and so on. Clinical effects of some drug pairs were influenced by the dosage ratio and administration ways such as oral combined externally application. This systematic review provided valuable information on the evaluation of clinical safety about eighteen incompatible medicaments,and it might be helpful to provide scientific supports and instructions for the clinical practices of the related Chinese herbal medicine.- 0
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发布时间：2024-09-23 - 摘要：<正>中华中医药学会中药鉴定分会定于2010年7月在陕西省咸阳市召开"全国第十次中药鉴定学术研讨会",会期3天。会议将邀请著名专家及相关人士就中药鉴定与品质评价、中药质量标准化、信息化、网络化进展情况;中药资源开发及可持续利用、中药鉴定学和生药学教学及实验、教材与标本等问题做专题报告。
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发布时间：2024-09-23 - 摘要：Objective: This paper gives an overview of chemical and pharmacological reasearch in Arillus Longan. Method: The chemical constituents and pharmacological activites of Arillus Longan were summarized and analyzed based on the related references which were published in domestic and overseas in recent years. Result and Conclusion: The Arillus Longan contains many kinds of active chemical constituents and has various pharmacological activites. This article provies valuable references for the further reasearch and development of Arillus Longan.关键词：Arillus Longan;pharmacological;chemical composition
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发布时间：2024-09-23 -
Analysis and Thought on Eighteen Incompatible Medicaments Based on the Clinical Usage in Modern Time
摘要：To explore the basic rules of clinical application about eighteen incompatible medicaments contained in prescriptions in modern time. 1964 formulas were screened out of TCM journal databases and studied based on the method of frequency analysis and maximal frequent item sets mining. The result showed that the prescriptions existed in all kinds of clinical trials,and were mainly used to treat the difficult and complicated cases such as cardio-cerebrovascular disease,cancer,and so on. Clinical effects of some drug pairs were influenced by the dosage ratio and administration ways such as oral combined externally application. This systematic review provided valuable information on the evaluation of clinical safety about eighteen incompatible medicaments,and it might be helpful to provide scientific supports and instructions for the clinical practices of the related Chinese herbal medicine.- 0
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发布时间：2024-09-23 - 摘要：Objective: To analyze the cosmetics-related mechanism of salvia miltiorrhiza and to lay the foundation for the relevant product development. Method: The cosmetics-related mechanism of salvia miltiorrhiza is summarized based on the related literatures and the application prospects of salvia miltiorrhiza in the field of cosmetics are analyzed. Result ＆ Conclusion: Salvia miltiorrhiza has the function of activating blood circulation to remove blood stasis and the function of removing heat from the blood to treat boils. Studies have confirmed that it contains a number of chemical constituents and pharmacologically effective. It is an ideal raw material of traditional Chinese medicine for TCM cosmetics.关键词：Salvia miltiorrhiza;TCM cosmetics;mechanism
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发布时间：2024-09-23 - 摘要：Objective: This paper gives an overview of chemical and pharmacological reasearch in Arillus Longan. Method:The chemical constituents and pharmacological activites of Arillus Longan were summarized and analyzed based on the related references which were published in domestic and overseas in recent years. Result and Conclusion: The Arillus Longan contains many kinds of active chemical constituents and has various pharmacological activites. This article provies valuable references for the further reasearch and development of Arillus Longan.关键词：Arillus Longan;pharmacological;chemical composition
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发布时间：2024-09-23 - 摘要：Objective:To analyze the cosmetics-related mechanism of salvia miltiorrhiza and to lay the foundation for the relevant product development. Method: The cosmetics-related mechanism of salvia miltiorrhiza is summarized based on the related literatures and the application prospects of salvia miltiorrhiza in the field of cosmetics are analyzed. Result & Conclusion:Salvia miltiorrhiza has the function of activating blood circulation to remove blood stasis and the function of removing heat from the blood to treat boils. Studies have confirmed that it contains a number of chemical constituents and pharmacologically effective. It is an ideal raw material of traditional Chinese medicine for TCM cosmetics.关键词：Salvia miltiorrhiza;TCM cosmetics;mechanism
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发布时间：2024-09-23 - 摘要：<正>蟾酥是蟾蜍皮肤腺及耳后腺中的白色分泌液,含有大量的蟾蜍色胺、5-羟色胺、5-甲基-5-羟色胺[1],能够杀死微生物防御天敌,其中起主要毒性作用的是蟾蜍配基B和蟾蜍毒素[2]。本研究采用纸片琼脂扩散法观察了蟾酥对伤寒杆菌的抑制作用。1材料与方法
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发布时间：2024-09-23 - 摘要：<正>异位妊娠是威胁生育期妇女生命的重要原因之一。近年发病率呈上升趋势,其最常发生的部位是输卵管,占异位妊娠95%左右。我院将101例未破裂型输卵管妊娠患者行腹腔镜及药物保守治疗的疗效报道如下:1资料与方法1.1一般资料2007年1月至2008年12月101例输卵管妊娠入院采用保守性治疗,经介绍两种治疗方法后,其中选择腹腔镜手术治疗56例,药物治疗45例,两组年龄、孕产次、停经时间,B超示包块直径、β-HCG等无统计学差异,诊
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发布时间：2024-09-23 - 摘要：<正>苦丁茶（Ilex kudingchaC.J.Ting）是我国南部和西南部民间一种常用植物,民间使用历史悠久,其主要分布在桂、粤、黔、琼等地,具有清热解毒、杀菌消炎、健胃消积、止咳化痰、生津止渴、提神醒脑、明目益智和抗辐射、抗衰老、活血脉、调节血脂等功效[1～2]。苦丁茶应用目前大多集中在嫩叶,对其他老叶弃之不用,但广西天等县大新县等地区民间
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发布时间：2024-09-23 - 摘要：<正>金银花为临床常用中药,主产于河南、山东。另有山银花产于广西、四川、云南。主治外感风热或温病发热,中暑,热毒血痢,痈肿疔疮,喉痹,多种感染性疾病。随着防治甲流中药处方的出台,金银花的价格短时间急升。然而,在对价格疯长的关注之余,药材质量的优劣与稳定性值得我们调查研究进行监控,现对我国大型药材市场之一的亳州药材市场
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发布时间：2024-09-23 - 摘要：<正>作者在社区中心门诊中,对37例确诊为压力性遗尿,中医辨证属于脾虚气陷肾气不固症见腰膝痠软,畏寒肢冷,气短乏力,或纳差、便溏,舌体淡胖,苔白,脉沉细弱等症者,予以自拟升阳缩尿汤中药辅以功能锻炼进行了治疗和观察。报告如下:1资料与方法1.1一般资料37例压力性遗尿患者均为2006年3月～
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发布时间：2024-09-23 - 摘要：<正>胃及十指肠溃疡发病率较高,其病程长,反复发作而且不易痊愈,多年来实践证明,其发病的主要原因为幽门螺杆菌致病。我们采用和胃治疡汤加减治疗胃、十二指肠溃疡140例,并用西药对比观察。现报告如下。1临床资料1.1一般资料280例胃及十二指肠溃疡病患者,均为本院门诊及病房2004年6月～2008年6月的患者,采用随机抽
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发布时间：2024-09-23 - 摘要：<正>老年容易发生便秘,作者临床观察了麻仁润肠丸对老年人便秘的疗效,并与果导片做了比较,现报道如下:1资料与方法1.1一般资料2009年1月～12月于我院就诊的便秘老年人120例,男51例,女69例,年龄62～88岁,病程1～6年,诉大便间隔时间3～11（6.3±2.5）d。1.2纳入标准老年患者,符合便秘的诊断[1]:每周排便少于3次,主诉排便费力,排便不净,粪块质硬或成硬球状。
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发布时间：2024-09-23 - 摘要：<正>近年来白血病发病呈上升趋势,其治疗仍是以西药化疗为主,不仅如此,化疗也是临床治疗其他恶性肿瘤延长癌症病人生存期的重要手段之一,但多数化疗药物对静脉内膜有强烈的刺激作用,可引起静脉炎,表现为患肢局部灼热剧烈的疼痛,肿胀麻木,沿静脉走向出现条索装红线,严重者活动受限。静脉炎给病人带来极大的痛苦,甚至影响治疗。为
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发布时间：2024-09-23 - 摘要：<正>口腔溃疡是肿瘤化疗后的常见并发症,笔者依据中药的传统功效和现代医学药理作用研制了溃疡平软膏,在临床应用中效果颇佳。1资料与方法1.1一般资料选择2007年5月—2009年5月在我院在化疗后发生口腔溃疡的患者200例（对芦荟过敏者除外）为研究对象,年龄10～60岁,其中男126例,女74例,Ⅰ度58例,Ⅱ度93例,Ⅲ度43例,Ⅳ度6例,随机分治疗组120例,
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术期刊光盘版"全文收录期刊;并被评为"中国中医药优秀期刊"及"中国学术期刊优秀期刊"。本刊创刊于
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发布时间：2024-09-23
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