最新刊期
卷 18 ， 期 11 ， 2012
- 摘要：Objective: To evaluate objectively the effectiveness and safety of intergration of Zhengqing Fengtongning and methotrexate on treatment of rheumatoid arthritis. Method: Sesrching pubished literature database through Vip, CNKI,Wanfang,CMCI＼CMCC,MEDLINE,Science Direct,and SpringerLink Resources;'rheumatoid arthritis’'arthritis’ 'rheuma-toid diseases’as index terms.Randomized controled trail on treatment of rheumatoid arthritis by intergration of Zhengqing Fengtongning and methotrexate on treatment of rheumatoid arthritis was taken as object of study,statistical analysis was carried on through Rev-Man 5.0 software offered by Cochrane. Result: The total enrolled number of patiens is 735 in 8 randomized controlled trials(RCT). The raise rate of each focus poinr is total effective [MD0.31,95%CI(0.18,0.52) P<0.000 1];Morning stiff[MD-29.66, 95%CI(-36.86,-22.4),P<0.000 01];erythrocyle sedimentation rate (ESR)[MD-4.87,95%CI(-6.66,-3.08),P<0.000 01]; rheumatoid factor(RF)[MD16.29,95%CI(14.71,17.88),P<0.000 01];CRP[MD-3.88,95%CI(-4.69,-3.06),P<0.000 01]. Conclusion: The results of this system review show that the intergration of Zhengqing Fengtongning and methotrexate can improve the sign and laboratory indexes and much better than that of methotrexate alone on rheumatoid arthrtis.At the same time,it reduce adverse effects.关键词：rheumatoid arthritis;Zhengqing Fengtongning;randomized controled trails;system review
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发布时间：2024-01-04 - 摘要：Objective: To obtain the effect and safety of Jinshuibao capsule in treatment of patients with chronic renal failure(CRF). Method: We searched PubMed(1966—2006), CNKI(1994—2011) databases and so on,and retrieved by computer and manual search to collect randomized controlled trial(RCT) on Jinshuibao capsule in treatment of chronic renal failure from 1979 to 2011.Two researchers independently executed a data extraction according to the inclusion criteria of Trials and quality assessment, Meta analysis was carried out. Result: A total of 8 trials involving 710 patients were included in the meta-analysis,which indicated that Jinshuibao capsule combined with conventional treatment in curing of chronic renal failure was more effective in ameliorating renal function and improving the quality of life than only using Jinshuibao capsule.These indexes included as follows:blood urea nitrogen (BUN)[MD-2.23,95%CI(-2.60,-1.85),P<0.000 01];serum creatinine(SCr)[MD-40.71,95%CI(-45.68,-35.75),P<0.000 01];24 hpro[MD-0.17,95%CI(-0.31,-0.03),P=0.02]; creatinine clearance rate(CCr)[MD-25.71,95%CI(-26.36,-25.06),P<0.000 01]。 Conclusion: Based on conventional treatment, Jinshuibao capsule can effectively relieve the progress of renal function in patient with chronic renal failure and has low side-effect,but further research work should be needed.关键词：chronic renal failure;Jinshuibao capsule;system evaluation;Meta analysis
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发布时间：2024-01-04 - 摘要：Objective: To optimize in vitro transdermal test conditions of Xianglian ointment. Method: Drug percutaneous permeability tester was used,the content of total alkaloids in different time,accepting liquid,sampling volume and mouse skin test were determined by UV-spectrophotometry.The cumulative transmittance was calculated to optimize in vitro transdermal test conditions of Xianglian ointment. Result: Optimized in vitro transdermal test conditions were:sampling volume of Xianglian ointment was about 0.1 g, 30% ethanol as receiving liquid, rat skin with cutin layer as medium;UV spectrophotometry was used to determine the content of total alkaloid with wavelength 345 nm. Conclusion: This optimized test method was simple,feasible and stable.关键词：Xianglian ointment;percutaneous penetration;UV spectrophotometry;total alkaloids
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of catalpol and stachyose from fresh Rehmannia glutinosa. Method: With extraction rate of catalpol and stachyose as indexes,the content of catalpol and stachyose were determined by HPLC.The content of catalpol and stachyose in different extraction methods were compared, and then extraction technology was optimized by L9(34) orthogonal test. Result: Compared traditional extraction method,flash-type extraction method had higher efficiency,optimal flash-type extraction technology was as follows: extracted 3 times with 6 times the amount of 20% ethanol,each time 1 min. Conclusion: Flash-type extraction process was an efficient and rapid method for extraction catalpol and stachyose from fresh R. glutinosa.关键词：fresh Rehmannia glutinosa;flash-type extraction;catalpol;stachyose;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study on application of statistical process control in pretreatment production process of Chinese medicine with pretreatment production process of Gardenia jasminoides from Qingkailing injection as carrier. Method: Univariate statistical process control for extraction ratio of geniposide was researched by Shewhart control chart and EWMA control chart;Based on principal component analysis model,multivariate Hotelling T2 and SPE control charts for pretreatment process of G. jasminoides were built;Technology process was diagnosed by contribution chart. Result: Shewhart and EWMA control charts could be achieved monitoring quality parameters in pretreatment technology of G. jasminoides; Multivariate statistical process control chart could be successfully applied in monitoring multiple process parameters; process analysis and fault diagnosis could be achieved by combining with contribution chart. Conclusion: Combination of univariate and multivariate statistical process control could be well monitored and understood of production process for traditional Chinese medicine.关键词：statistical process control;control chart;Gardenia jasminoides;production process;principal component analysis;process diagnosis
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of polysaccharide from roots of Arctium lappa. Method: Polysaccharide was extracted by water extraction and alcohol precipitation method with yield of polysaccharide as index;Response surface methodology was used to study on influencing factors of determination of polysaccharide from roots of A. lappa,response surface and contour were made with extract ratio of polysaccharide as response value. Result: Optimum extraction technology conditions were solid-liquid ratio of 1 : 14.43, extraction temperature 84.85 ℃,extracting time 3.81 h.Under these conditions,yield of polysaccharide from roots of A. lappa was 6.16%. Conclusion: This optimized technology was stable and feasible.关键词：roots of Arctium lappa;polysaccharide;extraction;response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To optimize separation and purification technology of total flavonoids from Glycyrrhiza uralensis by macroporous adsorption resin. Method: Twelve different types of resin were selected with the amount of absorption and desorption ratio of total flavonoids as indexes,based on this,separation and purification technology of total flavonoids were optimized. Result: Purification effect of AB-8 type macroporous was the best, its optimum technology conditions were:the concentration of sample solution 0.2 g·mL-1, maximum sample quantity 2 mL to 1 mL resin, velocity of absorption 0.5 BV·h-1, pH of sample solution 6, 6 BV water to remove impurities at 1 BV·h-1, eluted by 4 BV 70% ethanol at flow rate of 1 BV·h-1.Under this optimized process,purity of total alkaloids in elution was 38%. Conclusion: This optimized process was rational, feasible and suitable for industrial production.关键词：Glycyrrhiza uralensis;total flavonoids;liquiritin;macroporous adsorption resin;purification
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发布时间：2024-01-04 - 摘要：Objective: To optimize separation and purification process parameters of total saponins from Bolbostemma paniculatum by macroporous resin. Method: With absorption and desorption rate of total saponins as indexes,separation and purification performance of macroporous resin on total saponins from B. paniculatum was compared by static and dynamic test,optimum resin was selected and its purification technology was optimized. Result: HPD-750 macroporous resin showed the best property for purification of total saponins.its optimal process conditions were:diameter-height ratio 1 : 5, the concentration of sample solution 1.6 g·mL-1,maximum sample volume 13 mL, eluted by 1 BV water and 8 BV 95% ethanol,flow rate of adsorption and elution 1 mL·min-1,eluent were collected,ethanol was recovered,under these conditions,the content of total saponins was 63.53% by UV. Conclusion: This optimized process was simple and feasible to separate and purify total saponins from B. paniculatum effectively.关键词：Bolblstemma paniculatum;total saponins;macroporous resin;separation and purification
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发布时间：2024-01-04 - 摘要：Objective: To investigate purification technology of Fengbingkang mixture with chitosan as clarification agent. Method: With the content of baicalin from purified liquid as index,flocculation clarification process was optimized by orthogonal design,and compared with traditional ethanol precipitation process. Result: These test result showed that optimized technology was better than ethanol precipitation technology.Optimum flocculation clarification process conditions were:water extraction liquid concentrated to 1 : 8, the amount of chitosan 15%, temperature 50 ℃. Conclusion: Chitosan clarifying agent could be used for purifying Fengbingkang mixture.关键词：chitosan;orthogonal test;Fengbingkang mixture;purification technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize molding process of Kunshen dispersible tablet. Method: With disintegration time as index,type of excipients were optimized by single factor test and the amount of excipients was optimized by uniform design test. Result: Optimum formulation was composed of extract powder 65%, microcrystalline cellulose 21%, cross-linked polyvinyl polyrrolidone (PVPP) 12%, aspartame 2%.Under these conditions,disintegration time of formulation was less than 3 min,this tablet was dissolved quickly and completely. Conclusion: This optimized formulation was reasonable,and technology of it was feasible.关键词：Kunshen dispersible tablet;formulation technology;disintegration time;dissolution
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction process of Sanhu Tongfeng granule. Method: Orthogonal design was used to investigate the amount of water,extraction times and extraction time with the content of polydatin, emodin and yield of extract as indexes. The content of polydatin and emodin were determined by RP-HPLC, yield of extract was determined by drying method. Result: The best extraction conditions were as follows:refluxing extracted 2 times with 10-fold water,1 h for each time. Conclusion: This optimized extraction process was reasonable, stable, feasible and applicable to industrial production.关键词：Sanhu Tongfeng granule;polydatin;emodin;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To investigate percutaneous penetration effect of different concentration of volatile oil from Eugenia caryophyllata on ligustrazine phosphate (LP). Method: Modified Franz diffusion cell method was used,with azone as positive control,LP without penetration enhancers as negative control,penetration effect of 3%, 5%, 7% and 10% volatile oil from E. caryophyllata on LP through mouse skin were investigated,the content of LP was determined by HPLC. Result: Average cumulative permeation volume(Q12)of the 4 concentrations of volatile oil from E. caryophyllata on LP in 12 h were 3.030, 4.066, 1.845 and 1.205 mg·cm-2,respectively;and enhancing rates (ER) in 12 h were 1.54,2.06,0.96 and 0.62,respectively.Compared with Q12 of negative control group,5% and 3% volatile oil from E. caryophyllata had significantly enhancing effect. Conclusion: 3% and 5% volatile oil from E. caryophyllata could enhance permeation volume of LP through mouse skin obviously, and had great enhancing effect.关键词：volatile oil from Eugenia caryophyllata;ligustrazine phosphate patch;percutaneous penetration
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发布时间：2024-01-04 - 摘要：Objective: To evaluate in vitro release and transdermal absorption behavior of Guanxin gel ointment. Method: The content of salvianolic acid B was determined by HPLC,modified Franz diffusion cell method was applied to investigate in vitro transdermal absorption behavior of Guanxin gel ointment with isolated mouse skin as barrier. Result: In 24 hours, accumulative release rate of salvianolic acid B was 92.7%, accumulated transdermal rate of salvianolic acid B was 21.97%, transmission rate was 71.9 μg·cm-2·h. Conclusion: In vitro release and transdermal behavior of Guanxin gel ointment were all satisfied with the law of in vitro release and transdermal of Chinese medicine gel ointment.关键词：transdermal absorption;salvianolic acid B;HPLC;gel ointment
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发布时间：2024-01-04 - 摘要：Objective: To optimize molding process of Ganershu pellet. Method: With yield of pellets, roundness,bulk density and friability as indexes,prescription and technology in molding process of Ganershu pellet were investigated,pellet was prepared by fluidized bed side spray pelleting method. Result: Optimized pellet forming prescription and technology parameters were:relative density of liquid was 1.12-1.16 g·mL-1,6% talcum powder as antiadhesive agent,rotating rate of spray solution pump 4 r·min-1,atomizing pressure of 300 kPa, height of turntable of 5 mm,speed of turntable of 300 r·min-1,inlet air temperature of 75 ℃,fan frequency of 25-35 Hz. Appearance of Ganershu pellet prepared by fluidized bed side spray pelleting method were smooth with high roundness,yield of pellets was 86.7% with particle size between 600\|900 μm. Conclusion: This optimized molding technology of pellet was reasonable and stable.关键词：Ganershu pellets;fluidized bed;preparation process
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发布时间：2024-01-04 - 摘要：Objective: To optimize ultrasonic extraction technology of antitumor diterpenoid from Pteris dispar. Method: Four factors of the concentration of ethanol,extraction time,extraction times and solid-liquid ratio were investigated by L9(34) orthogonal design test,the content of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) was defined as index,the content of 5F was determined by HPLC. Result: A2B2C3D3 was optimum ultrasonic extraction technology,which was ultrasonic extracted 3 times with 12 times the amount of 75% ethanol by 65 min each time. Conclusion: This optimized process was stable and available.关键词：Pteris dispar;antitumor;diterpenoid;orthogonal test;ultrasonic extraction
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发布时间：2024-01-04 - 摘要：Objective: To optimize continuous countercurrent extraction process of Egui Yangxue prescription. Method: With the content of ferulic acid and the amount of extract as indexes,the content of ferulic acid was determined by HPLC,optimum continuous countercurrent extraction process of Egui Yangxue prescription was determined by multi-index comprehensive evaluation method and orthogonal design. Factors of extraction time,solid-liquid ratio, the concentration of ethanol and extraction temperature were investigated. Result: Optimum extraction process was:solid-liquid ratio of 1 : 16 (equivalent to each level liquid ratio of 1 : 4), extracted 20 min with 60% ethanol under extraction temperature of 40 ℃. Conclusion: This optimized extraction process was stable and reliable, and it could provide scientific basis for relative research of Egui Yangxue prescription.关键词：Egui Yangxue prescription;continuous countercurrent extraction;ferulic acid
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发布时间：2024-01-04 - 摘要：Objective: To optimize enrichment technology conditions of total flavonoids from leaves of Eriobotrya japonica by macroporous adsorption resin. Method: With rutin as reference substance,the content of total flavonoids was determined by aluminium nitrate chromogenic method.With static adsorption capacity and static desorption rate as indexes,optimum type of macroporous resin was selected by comparing adsorption and desorption effect of 6 kinds macroporous resin for total flavonoids from leaves of E. japonica, and to investigate its absorption and desorption conditions. Result: HPD 100 macroporous resin was the most suitable for purification of total flavonoids from E. japonica.Optimum purification conditions were:the concentration of sampling liquid of 3 g·L-1, sampling velocity 2 BV·h-1, sample liquid volume 2.5 BV,eluted by 2 BV deionized water and 5 BV 70% ethanol at the speed of 1 BV·h-1,eluate was collected.Under these process conditions,yield of total flavonoids was 78.7%,purity of total flavonoids was 47.3%. Conclusion: HPD 100 macroporous resin had a good purification effect of total flavonoids from leaves of E. japonica,optimized purification process was reasonable,stable and feasible.关键词：leaves from Eriobotrya japonica;total flavonoids;macroporous resin;HPD 100;purification
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发布时间：2024-01-04 - 摘要：Objective: To determine the contents of four kinds of anthraquinone components in Xinqingning tablet by quantitative analysis of multi-component with a single-marker(QAMS) and external standard method(ESM) respectively,and compare results by this two methods. Method: RP-HPLC determination was performed at 30 ℃ with Accurasil C18 column (4.6 mm×250 mm, 5 μm), mobile phase of methanol-0.4% phosphoric acid (85 : 15), detection wavelength at 254 nm, and flow rate of 1.0 mL·min-1. Result: There was no significant difference between calculated value of QAMS and measured value of ESM for four kinds of anthraquinone components from Xinqingning tablet,RSD<5%. Conclusion: Under certain chromatographic conditions,relative correlation factor could be used as a constant for quality evaluation of Rheum palmatum preparation which was determined by QAMS.
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发布时间：2024-01-04 - 摘要：Objective: To determine bufothionine in different parts of the dried toad skin from two species. Method: A quantification method was developed by HPLC with photodiode array (PDA) detection. Bufothionine was identified unambiguously by HPLC-DAD 3D and HPLC-MS-MS analysis in comparison with reference standard. The extraction method was optimized as 50 times 50% MeOH ultrasonic 60 min. The analysis was performed on WondsilTM C18 column (4.6 mm×250 mm, 5 μm) with ACN-H2O (6 : 94) as mobile phase and detection wavelength was at 226 nm. Result : The samples were analyzed with good linear regression relationship (r=0.999 7), precisions (RSD, 0.59%), repeatability (RSD, 1.56%), stability (RSD, 0.73%) and recovery (98.57%; RSD, 2.03%). Bufothionine was detected in different parts of toad skin from Bufobufo gargarizans cantor and Bufo melanostictus Schneider. The contents were from 0.07% to 0.17%. Conclusion : The content of bufothionine revealed no significant differences among the different parts in these two species of toad skin.关键词：bufothionine;HPLC;quantification;toad skin
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发布时间：2024-01-04 - 摘要：Objective: To study the changes of low molecular weight saccharide in Rhizoma Polygonation during processing. Method: HPLC analysis was performed on a BIO-RAD Aminex HPX-87C column (7.8 mm×300 mm, 9 μm), and the mobile phase was water. The flow rate was 0.4 mL·min-1 and the temperature of the ELSD was 105 ℃. Result: Sucrose and fructose were detected in the three species of Rhizoma Polygonation raw products but not glucose, and which was detected after processed to 8 h or 16 h on the species; the total contents of reducing sugar and low molecular weight saccharide were the highest when processed to 16 h which were 4-27 times higher than those of the raw products. Conclusion: The composition and the contents of low molecular weight saccharide in three species of Rhizoma Polygonation varied during processing.关键词：Rhizoma Polygonation;HPLC;processing;low molecular weight saccharide;content
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发布时间：2024-01-04 - 摘要：Objective: To analyze the contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of Chrysanthemum indicum, which sources from hengyang, Hunan province. Method: Chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of C. indicum were extracted by the method of ultrasonic method with 80% ethanol and analyzed by RP-HPLC at the wavelength of 327 nm with the flow rate of 1.0 mL·min-1. Meanwhile, 0.05% H3PO4 aqueous solution and acetonitrile were used as the mobile phase with gradient elution. Result: The linear range (n=5) of chlorogenic acid (r=0.996)and 3,5-O-dicaffeoylquinic acid(r=0.992) was 10-100 mg·L-1. The average recoveries (n=6) of chlorogenic acid and 3,5-O-dicaffeoylquinic acid were 98.5% and 99.7%,respectively. The contents of chlorogenic acid in the different parts of C. indicum including leaves, flowers, buds, tender stems and old stems was 1.08%,0.44%,0.39%,0.58% and 0.52%, respectively, while the contents of 3,5-O-dicaffeoylquinic acid was 1.66%,1.01%,0.82%,0.93% and 0.64%, respectively. The contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the leaves are the highest of the all tested samples. Conclusion: Different parts of C. indicum tested all have chlorogenic acid and 3,5-O-dicaffeoylquinic acid, but the contents of which was obvious difference.关键词：Chrysanthemum indicum;chlorogenic acid;3,5-O-dicaffeoylquinic acid;RP-HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a new RP-HPLC method for simultaneous determination of salvianolic acid A, salvianolic acid B, tanshinol, lithospermum acid, tanshinone ⅡA in crude extract of Radix Salvin Miltiorrhiza. Method: Analysis was carried out on a Kromasil 100-5 C18 (4.6 mm×250 mm,5 μm) column with acetonitrile-pure water(added 0.1% formic acid) as mobile phases in gradient elution. The flow rate was 1 mL·min-1, and the UV detector was monitored at 285 nm. Result: All calibration curve showed good linear regression in test ranges(r>0.999 5); and the overall recoveries were in the range of 97%-103%(n=3)with RSD less than 3%. The overall RSD of precision test were less than 3%. Conclusion: The method is rapid,accurate,credible and repeatable,and can provide bases for the material basis and compatibility for Radix Salvin Miltiorrhiza.关键词：HPLC-UV;salvianolic acid A;salvianolic acid B;tanshinol;lithospermum acid;Tanshinone ⅡA;content determination
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发布时间：2024-01-04 - 摘要：Objective: To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Rubus parvifolius. Method: The separation was achieved on a Kromasil C18 column (4.6 mm×250 mm, 5 μm) at 30 ℃ using methanol-phosphoric acid solution (90 : 10) as the mobile phase at a flow rate of 1.0 mL·min-1, 210 nm as the detection wavelength. Result: The linear ranges of oleanolic acid and ursolic acid were from 0.136 8 to 1.368 0 μg and 0.212 8 to 2.218 0 μg, respectively. The average recoveries (n=6) for oleanolic acid and ursolic acid were 97.5%(RSD 1.6%), 96.9% (RSD 1.4%), respectively. Conclusion: The proposed method is accurate, simple and applicable for determination of oleanolic acid and ursolic acid in R. parvifolius.关键词：Rubus parvifolius;oleanolic acid;ursolic acid;RP-HPLC;content determination
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发布时间：2024-01-04 -
Simultaneous Determination of Four Active Components in Baige Capsules by HPLC and Monolithic Column
摘要：Objective: a method of simultaneous and fast determination the contents of puerarin, daidzein, imperatorin and isoimperatorin in Baige capsules was established. Method: the monolithic C18 column was used to perform the separation, and the mobile phase was methanol (A)-0.1% glacial acetic acid (B) with gradient elution, the flow rate was 1.0 mL·min-1, the detection wavelength was at 254 nm and the injection volume was 5 μL, the column temperature was 30 ℃. Result: these four components could be well separated under the established chromatographic condition, the linear correlation of each component was fine in certain concentration(r>0.999 5), the recovery was between 95%-105%. Conclusion: the established method was simple, rapid and reliable and could be successfully applied in the active component detection in Baige capsules.关键词：puerarin;daidzein;imperatorin;isoimperatorin;monolithic column- 2
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发布时间：2024-01-04 - 摘要：Objective: To study the impact of machine-boiling decoction on TCM prescription’s total extracts and active ingredients, to provide a basis for the scientific evaluation of the extracting machine and the theory of Chinese Herbal medicine or 'decocted later’ to provide a basis. Method: The classic prescription 'Liuwei Dihuang decoction’was used as the representative, and the content of the two decoctions was analyzed with RP-HPLC using paeonol as indicator was analyzed. This result was used to evaluate the quality of decoctions. Result: Paeonol in the decoctions of the machine-boiling was significantly higher than that of the traditional one. But the result is opposite to the total extracts. Conclusion: The significant quality difference of 'Liuwei Dihuang decoction’ is between the tradition-boiling method and the machine one.关键词：different decoction;Liuwei Dihuang Decoction;total extractives;paeonol
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality control standard of Qichan Koufu Jiechang Baxiang tablets. Method: Astragalus,corium bufon and flavescens were identified by TLC. The UPLC and HPLC in preparations for astragaloside Ⅳ, resibufogenin and cinobufagin, matrine are quantificationally analyzed. Result: The characteristic for identification by TLC was distinct and highly specific. The linear range of astragaloside Ⅳ was 0.233 6-0.817 6 μg(r=0.999 9),and the average recovery was 99.66%(RSD 1.14%). The linear range of resibufogenin concentration was 0.001 75-0.010 5 μg(r=1.000 0),and the average recovery was 100.44%(RSD 1.94%). The linear range of cinobufagin concentration was 0.003 51-0.021 06 μg(r=1.000 0),and the average recovery was 99.57%(RSD 2.73%). The linear range of Matrine concentration was 0.336 8-1.178 8 μg(r=0.999 8),and the average recovery was 100.30%(RSD 1.11%). Conclusion: The detection method is simple, reliable, reproducible and can be suitable for quality control of Qichan Koufu Jiechang Baxiang Tablets.关键词：Qichan Koufu Jiechang Baxiang tablets;TLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: The quantitative method was established of total saponins in Periploca forrestii and the content of the general saponins of Periploca forrestii from different places was determined. Method: Ursolic acid was selected as control article.Coloration was used with 5% vanillin and perchloric acid.The content of total saponins in P. forrestii was determined at 550 nm by UV. Result: The calibration curve was linear over the range of 9-55 mg·L-1 with the correlation of 0.999 2,and the linear regression equation was A=0.011 9 C+0.097 2.The average recovery of ursolic acid was 97.16% with RSD of 1.3%(n=6). The contents of the total saponins in P. forrestii from various habitats was quite different. Conclusion: The method is accurate,simple,sensitive and reliable. So it can be used to determine the content of total saponins in P. forrestii.关键词：Periploca forrestii;UV;total saponins;ursolic acid
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of carvone and limonene contents in caraway,research difference areas of carvone and limonene contents in caraway from different origin. Method: The analysis was carried out on a chromatographic column for HP-FFAP (PEG 20000, 0.20 mm×25 m, 0.33 μm); the column temperature 65-115 ℃, the carrier gas was N2, flow rate of 1.0 mL·min-1, detector FID, the inlet and the temperature detector were all setat 250 ℃. Result: The quality scores of carvone and limonene content in 5 batches of caraway from different origin were 50%-60% and 35%-42%. Conclusion: The method is simple, accurate, reproducible, and can be used for content determination of caraway. Different areas of caraway are small difference.关键词：caraway;limonene;carvone;content;GC
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发布时间：2024-01-04 - 摘要：Objective: To established an RP-HPLC method for determination of quercetin in Pyracantha fortuneana and to compare quercetin contents in different medicinal parts. Method: 90%methanol-25% hydrochloride aqeous solution (4 : 1) was used to extract quercetin and Hypersil ODS (4.6 mm×250 mm, 5 μm) column was used to analyze quercetin content with methanol-0.1% phosphoric acid aqueous solution as mobile phases. Flow rate was 1.0 mL·min-1. Detection wavelength was set at 360 nm. Result: The standard curve of quercetin was linear in the range of 0.048 5-1.552 0 μg.The average recovery of quercetin was 98.44%. Conclusion: Quercetin contents in different medicinal parts of P. fortuneana was different.关键词：Pyracantha fortuneana;RP-HPLC;quercetin;content detemination
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发布时间：2024-01-04 - 摘要：Objective: A high performance capillary electrophoresis(HPCE) method for the determination of paeonal in Jiegu Huayu pills was established. Method: 15 mmol·L-1 sodium dihydrogen phosphate-20 mmol·L-1 sodium borate buffer (pH 8.2) was used as running buffer. The applied voltage was 23 kV and the detection was set at the wavelength of 274 nm. Result: The linear ranges of the calibration curves for paeonal was 0.017 4-0.104 2 g·L-1 with a correlation coefficient of 0.999 8.The average recovery was 99.42% with RSD1.27%. Conclusion: The method is simple,rapid and reproducible,and it can be used for the quality control of determination of Jiegu Huayu pills.关键词：HPCE;Jiegu Huayu pills;paeonal
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发布时间：2024-01-04 - 摘要：Objective: To compare the chemical constituents of volatile oil from roots, stems and leaves of Sarcandrae Herba, to reveal the pharmacodynamic differences of Sarcandrae Herba in different parts and provide scientific basis for its quality control. Method: The essential oil was extracted by stream distillation. The constituents were separated and identified by GC-MS. The relative content of each constituent was determined by area normalization method. Result: Fifty-two components of volatile oil were identified from different parts of Sarcandra glabra, thirty-three, forty-one and thirty-nine compounds from the roots, stems, and leaves of Sarcandrae herba were identified, accounted for 83.99%, 83.85%, 93.32%of total essential oil, respectively, of which twenty-four were same, while twenty-eight were different. Conclusion: The chemical constituents of volatile oils were quite different in kinds and main component content from the roots, stems, and leaves of Sarcandrae Herba.关键词：Sarcandrae Herba;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: Establishing a method of HPLC for quantitative determination of formononetin in the effective fraction of Qishao formula to further characterize the content of isoflavones in the effective fraction of Qishao formula. Method: The procedure of HPLC-PDA was performed on the chromatographic column of ODS HYPERSIL(4.6 mm×250 mm,5 μm), and the mobile phase in the gradient elution program was acetonitrile-phosphoric acid solution(0.2%), the column temperature was at 35 ℃.The flow rate was 1 mL·min-1, and the detection wavelength was at 249 nm. Result: The regression equations was as follows: Y＝6×106+06X-16 320(r=0.999 8).Formononetin showed good linear relationship from 0.036 48 to 0.364 8 μg.The average recovery was 98.70%, (RSD 0.77%). The contents of formononetin in three batches of the the effective fraction of Qishao formula was 0.81,0.74,0.63 mg·g-1, respectively. Conclusion: The method is easy and accurate with higher repeatability, which can be used in the quality control of the effective fraction of Qishao formula. It will provide support to control of the isoflavones in the effective fraction of Qishao formula. At the same time to lay the foundation for controlling its quality effectively.关键词：Qishao formula;the effective fraction;formononetin;isoflavones;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To research the technologies of Litchi flavonoid separation and the flavonoid constituents. Method: The flavonoid content was detected with rutin as the standard reference by UV absorption, comparisons of the flavonoid extraction effects with ethanol and its acidification solutions were conducted, the technologies of litchi flavonoid separation with macroporous resin were optimized, and the flavonoid constituents of the extraction and separation products were analyzed with HPLC graph. Result: The UV absorption method to detect Litchi flavonoid content was simple and workable; the extraction effect of 70% of ethanol solution was better than its acidification solution, the flavonoid constituent was simpler than the acidification one, and its recovery rate and purity reached (92.5±2.0)% and (28.7±1.8)% respectively. Resin AB-8 had the best effect of flavonoid separation among the 6 macroporous resins, the recovery rate and the purity of the separated product with the eluant of 80% ethanol solution were (86.5 ±2.1)% and (68.6±1.5)% respectively. Conclusion: The technology of extraction with 70% ethanol followed by separation with resin AB-8 to extract and separate Litchi flavonoid was simple and workable.关键词：litchi;flavonoid;extraction and separation;macroporous-resin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: Determination of baicalein in Qinloukeli by HPLC. Method: The separation was performed on Alltech C18(4.6 mm×250 mm, 5 μm) at 25 ℃. The mobile phase was composed of methanol-water-phosphorous acid solution (47 : 53 : 0.2) with a flow rate of 1.0 mL·min-1. The detective wavelength is at 280 nm. Result: Hesperidin was linear within the range of 0.057 2-0.629 2 μg(r=0.999 9). The average recovery for hesperidin was 99%,and RSD was 1.23%. Conclusion: The method was selective, accurate and simple, and suitable for controlling the quality of Qinloukeli.关键词：Qinlou Keli;baicalein;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of the stems of Ilex pubescens. Method: The chemical constituents were isolated and purified via silica gel and Sephadex LH-20 column chromatography as well as recrystallization. Their structures were determined on the basis of spectral analysis and physicochemical properties. Result: Five compounds were isolated from the petroleum ether and EtOAc fractions.They were obtained and their structures were identified as ilexgein A( 1 ), ilexsaponin A1( 2 ),ilexsaponin B1( 3 ), Ilexsaponin B2( 4 ), β-sitosterol( 5 ). Conclusion: All of the five compounds were firstly isolated from the stems of I. pubescens.关键词：Ilex pubescens;stems;chemical studies;structural identification
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents of volatile oil from Ocimum basilicum collected in two different sources. Method: The volatile components were extracted by steam distillation method, and analyzed by GC-MS. Peak area normalization method was used for the relative content of volatile oil. TLC was used for the identification of two samples. Result: Twenty-nine components were identified in O. basilicum from Guangzhou, which accounted for 99.90% of the total relative content.The main components were p-allylanisole (83.082%), linalool 4.734%), tau-cadinol (2.715%), eucalyptol (2.251%). Thirty-one components were identified in O. basilicum from Bozhou, which accounted for 98.65% of the total relative content. The main components were linalool (26.91%), methyl cinnamate (21.85%), tau-cadinol (17.41%), ledol (14.154%). Two characteristic spots could not be identified in O. basilicum from Bozhou but from Guangzhou. Conclusion: The volatile oil from different sources had obviously different chemical constituents. GC-MS and TLC can be used for the identification and analysis of sources. It pointed out that O. basilicum should clear about its quality and sources before exploitation and application.关键词：Ocimum basilicum;volatile oil;GC-MS;TLC
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发布时间：2024-01-04 - 摘要：Objective: By comparing different extraction methods of genomic DNA, the best extraction method of Ephedra intermedia genomic DNA was selected. Method: Improved cetyltrimethyl ammonium bromide(CTAB) method, SDS method, kit method were used for the extraction of genomic DNA from E. intermedia. The yield and purity of total genomic DNA were examined by agarose gel electrophoresis, UV-spectrophotometry and ISSR-PCR. Result: Three extraction methods could extracted the high amount genomic DNA from fresh tissue of E. intermedia. Improved CTAB method could yield highest purity total DNA, and the electrophoretic bands of amplified products were obvious. On the other hand, SDS method and kit method indicated low purity of the yielded DNA and could not be used in downstream application directly. Conclusion: The improved CTAB method is the best of the three compared methods for extracting the genomic DNA from E. intermedia, which is suitable for PCR reaction and other molecular research in E. intermedia genomic DNA.关键词：Ephedra intermedia;DNA extraction;CTAB method
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for determination of trigonelline in Baogan granulas. Method: The content of trigonelline was determined by HPLC. The Agilent ZORBA×NH2 column (4.6 mm×250 mm, 5 μm) was used and mobile phase was composed of water-methanol-acetonitrile(20 : 40 : 40). The flow rate of mobile phase was 1 mL·min-1 and detection wavelength was at 265 nm and the column temperature was maintained at 40 ℃. Result: The linear response range was from 5-25 μL(r=0.999 9). The recovery rate was 98.3%(n=9), RSD was 1.63%. Conclusion: HPLC method is simple, accurate and exclusive, which can provide the scientific basis for quality control of the Baogan granulas.关键词：Baogan granulas;trigonelline;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: The establishment of infrared spectral identification for Cichorium glandulosum and C. intybus was carried out. Method: The use of infrared spectroscopy of three identification methods for C. glandulosum and C. intybus were analyzed and identified. Result: Infrared spectra showed that sugar and aromatic component content in the C. glandulosum was higher than C. intybus. Observing their secondary derivative spectra in the range of 1 800-1 300 cm- 1, the strongest peak of C. glandulosum is at 1 593 cm-1 and it is from the aromatic compounds, and the peak of C. intybus is at 1 660 cm-1 which from the protein amide I band, the intensity of peaks at 1 593, 1 509, 1 421, 1 377, 1 336 cm-1 of C. glandulosum is more different from C. intybus. In 1 200-700 cm-1, there have similar peaks at the 717, 713 cm-1, in addition, the strongest peak of C. glandulosum is at 1 063, 957 cm-1, more different from C. intybus. The distinction of 2D IR correlation spectra is more obvious. In 1 800-1 500 cm-1, the stronger automatic peaks of C. glandulosum present at 1 590, 1 660 cm-1, respectively, but those of C. intybus have a very wide area auto peak in the 1 700-1 500 cm-1, respectively. In 1 120-880 cm-1, C. glandulosum has three automatic peaks while C. intybus has two automatic peaks. Conclusion: The infrared spectra of three identification method are simple, fast, can be used in the identification of C. glandulosum and C. intybus.关键词：C. glandulosum and Cichorium intybus;general infrared spectroscopy (FTIR);secondary derivative spectra;two dimensional correlation;infrared spectroscopy (2D-IR)
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发布时间：2024-01-04 - 摘要：Objective: The purpose of our study was to investigate mechanism of action of Kang’ai Fuzheng Prescription (KFP) in the human hepatoma cells SMMC-7721. Method: The anti-proliferative potential of KFP was assessed using MTT assay. Cell apoptosis and the apoptosis-related protein activation of PI3K /AKT pathway in human hepatoma cells SMMC-7721 were evaluated by Western blot after treatment with KFP. Result: KFP suppressed the proliferation of human Hepatoma Cells SMMC-7721 in a time and dose-dependent manner. Western blot showed that the activation of caspase-9, upregulation of phosphstase and tensin homolog deleted on chromosome ten(PTEN) and downregulation of p-AKT in KFP-treated human Hepatoma Cells SMMC-7721 were observed. Conclusion: Our results showed that KFP may suppress constitutively activated targets of phosphatidylinosito-I-3-kinases (PI3K) and AKT, whereafter activate caspase-9 in the human Hepatoma Cells SMMC-7721 through increasing the activation of PTEN, which suggested that PI3K/AKT pathway plays an important roles in KFP-induced apoptosis of human Hepatoma Cells SMMC-7721.关键词：KFP;PI3K/AKT pathway;apoptosis;human Hepatoma Cells SMMC-7721
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determination of quercetin in Yuxingcao dispensing granules. Method: Orthogonal experiments were used to optimize extraction technology. The samples were separated on the Hypersil ODS C18 (4.6 mm×250 mm,5 μm) column which was eluted with methanol-0.2% phosphoric acid solution (45 : 55)with detective wavelength at 372 nm and flow rate at 1 mL·min-1,column temperature at 30 ℃. Result: The method had good linear relationship within the range of 0.09-1.44 μg(r=0.999 9)for quercetin. The average recovery rate was 100.6% with RSD of 1.68%. Conclusion: The method is simple,rapid and reliable which can be used to determin quercetin in Yuxingcao dispensing granules.
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发布时间：2024-01-04 - 摘要：Objective: To study Banlangen levels of adenosine in different dosage forms, and compare betweendifferent anti-inflammatory agents. Method: HPLC determination of Radix syrup, Banlangen, Radix soft capsules,tablets Radix, Radix Pill formulations of adenosine content column for the Dia-monsil Cl8 (4.6 mm×200 mm,5 μm),detection wavelength of 260 nm, flow phase: methanol - water (10 : 90), flow rate: 1.0 mL·min-1, column temperature 30 ℃. After the administration of the other mice, xylene-induced ear swelling coated, comparing the group differences in degree of swelling. Result: Adenosine in the range of 0.016 0-0.160 0 g good linear relationship ( r = 0.999 7),the average recovery was 98.7%, RSD 0.79% ( n = 9). Radix soft capsules in which the highest concentrationsof adenosine, followed by the Banlangen, board Radix tablets, pills Radix, Radix syrup. In xylene-induced mouse ear edema model of anti-inflammatory, anti-inflammatory effect Banlangen best, followed by the Radix softcapsules, pills Radix, Radix film, Radix syrup. Conclusion: The method precision, good reproducibility, accurate,and efficacy of adenosine has some relevance but not absolute, the other anti-inflammatory agents may produce asynergistic ingredients.关键词：Radix preparations;adenosine;anti-inflammatory
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发布时间：2024-01-04 - 摘要：Objective: To explore the influence of Qiju Dihuang decoction on Caspase-3 expression in N-methyl-N-nitrosourea (MNU)-induced retinal degeneration rats. Method: Sprague Dawley rats were divided into three groups: the control group, the model group and the Qiju Dihuang decoction group. MNU at dose 40 mg·kg-1 was ip injected at 50 d after birth to induce retinal degeneration model. All rats were sacrificed at scheduled time points.The expression of Caspase-3 on the retina was detected by immuohistochemistry and Real-Time PCR. Result: The results were demonstrated by immuohistochemistry and Real-Time PCR. Positive Caspase-3 expression in the model group was detected at 12 h after ip MNU, reached to the peak at 2 d and gradually reduced, whereas Caspase-3 expression in the Qiju Dihuang decoction group reached to the peak at 3 d and the peak value was lower than the former. Conclusion: The positive expression of Caspase-3 was raised after MNU injection. Qiju Dihuang decoction can restrain the apoptosis of photoreceptor cells by decreasing the expression of Caspase-3.关键词：Qiju Dihuang decoction;retinal degeneration;Caspase-3;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the regulating effect and mechanism of the Tibet Rhodiola crenulata on blood pressure in spontaneous hypertension rat (SHR). Method: The male SHR of 13 weeks old were randomly divided into five groups: SHR control group(0.5%CMC), the high, middle, low dosage group of the R.Crenulata(crude drug 1.08, 0.27, 0.068 g·kg-1·d-1) and captopril group(0.015 g·kg-1·d-1).Twelve male Wistar-kyoto rats(WKY)were served as normal group(0.5%CMC).Different dose of Tibet R.Crenulata were administrated daily via gavage for 4 weeks.Systolic blood pressure(SBP) were measured respectively by tail-cuff technique before therapy,at the time after a single ig administration of Tibet R.Crenulata and at the time after amultiple ig adiministration. At end of the experiment, blood lipid parameters, the content of nitric oxide(NO),the activity of superoxide dismutase(SOD) were also detected and the ratios of left ventricle wet weight/body weigh left ventricutar mass index(LVMI) were calculated. Result: The SBP level of SHR in R. crenulata treatment groups was depressed notably(P<0.05-P<0.01),nevertheless the SBP level of SHR of model group was increased obviously comparing with normal group (P<0.01) at the time after a single ig administration of R. crenulata for 12 h and at the time after amultiple ig adiministration of Tibet R.Crenulata for 4 weeks. Compare with model group.The level of LDL-C was decreased significantly(P<0.05-P<0.01) in Rhodiola treatment groups.The level of HDL-C was obviously increased and the level of FFA was decreased significantly.The level of TC was decreased(P<0.05)in low-dose group of R.crenulata.The content of NO were increased significantly in high-dose and middle-dose group of R.Crenulata.The activity of SOD were increased significantly (P<0.05-P<0.01)in high-dose and low-dose group of R.Crenulata.LVMI were decreased significantly in R.Crenulata treatment groups. Conclusion: The hypotensive function of R. crenulata was significantly in SHR. Its mechanism may modulate the lipid metabolism,increase the content of NO,enhance the activity of SOD and improve left ventricular hypertrophy.关键词：Tibet Rhodiola crenulata;spontaneously hypertensive rats;NO;SOD
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effects of Huanglian Jiedu decoction against Pseudomonas aeruginosa biofilms and its synergistic antibacterial in combination with azithromycin. Method: The microdilution assay was used to detect MIC of Huanglian Jiedu decoction against P. aeruginosa, checkerboard method was used to examine synersism of Huanglian Jiedu decoction and azithromycin. MTT assay was used to detect SMIC of Huanglian Jiedu decoction against P. aeruginosa biofilms, and microscopes was used to observe morphology of biofilms treated with the two drugs. Result: MIC of Huanglian Jiedu decoction against P. aeruginosa was 100 g·L-1and azithromycin 200 mg·L-1. After the combination of two-drug MIC was 25 g·L-1 and 25 mg·L-1fractional inhibitory conceritration(FIC) 0.1875, which showed a synergismwith azithromycin.SMIC50 of Huanglian Jiedu Decoction against P. aeruginosa biofilms at 1,3, 7 d was 15.6, 15.6, 31.25 g·L-1, respectively; SMIC80 of the biofilms was 250, 250, 250 g·L-1, respectively. Morphologically Huanglian Jiedu decoction inhibited P. aeruginosa biofilms evidently at the concentration of SMIC80. Conclusion: Huanglian Jiedu decoction could inhibit P. aeruginosa biofilms, and has a synergism with azithromycin.关键词：Pseudomonas aeruginosa;biofilms;Huanglian Jiedu Decoction;synergistic antibacterial
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发布时间：2024-01-04 - 摘要：Objective: To investigate antibacterial activity by Periplaneta americana skimmed cream and active carbon material, and compare the effect of intensity between P. americana skimmed cream and active carbon material. Method: The P. americana skimmed cream was decolored with active carbon and solid bleaching material was obtained after freeze-drying. The antibacterial activity of them against Staphylococcus aureus (S.aureas), Staphylococcus albus (S.albus), Escherichia coli (E.coli), Shigella dysenteriae (S.dysenteriae), Salmonella Paratyphi (S.paratyphi A), S.paratyphi B, Pseudomonas aeruginosa (P. aeruginosa), Bacillus bacteria (B.subtilis), Proteus (P.vulgaris), Candida albicans (C.albicans), S. aureus (ATCC 25923), E. coli (ATCC 25922) in vitro was observed, the minimum inhibitory concentration (MIC) was determined. Result: In all experimental strains, only skimmed cream had some antibacterial effect,S. albus.MIC values of 31.25 g·L-1, on the other multiple strains of bacteria had no antibacterial activity. active carbon material and antibacterial against S. aureus 25923, C. albicans, no antibacterial activity against many other strains of bacteria had antibacterial activity, in which the S. albus and P. aeruginosa antimicrobial was better, the MIC was 15.63,62.5 g·L-1,respectively. Conclusion: P. americana contains antimicrobial active ingredients, compared with active carbon material and skimmed cream, antibiotic effect of active carbon material is better than that of skimmed cream.关键词：Periplaneta americana;skimmed cream;active carbon material;in vitro antibacterial activity;minimum inhibitory concentration
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Qize decoction at endoplasmic reticulum stress(ERS) in rats with chronic renal failure(CRF). Method: SD rats were fed with adenine of 2.5% to establish chronic renal failure model. They were divided into five groups:normal group, model group, Niaoduqing group(2.5 g·kg-1·d-1), Qize decoction of high dosage(50.4 g·kg-1·d-1)group and Qize decoction of low dosage(12.6 g·kg-1·d-1) group,each group has 10 rats. All groups were infused with adenine into stomach in addition to normal group, at the same time,each rat in chinese herbal compound group was also infused with chinese herbal compound into stomach and Niaoduqing group was given Niaoduqing liquid for 24 days. Body weight was observed dynamically, blood urea nitrogen(BUN) and serum creatinine(SCr) levels were checked by automatic biochemical analyzer.The apoptosis of renal was evaluated by means of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL).The expression of C/EBP-homologous protein(CHOP) and Caspase-12 was detected by Western bloting. Result: Compared with the control group BUN(4.19±0.77)mmol·L-1 and SCr(15.89±2.62)μmol·L-1,in the model group BUN(17.21 ±4.63) mmol·L-1 and SCr levels (67.00 ±25.62) μmol·L-1 were much higher(P<0.01).The results of TUNEL showed that the few numbers of apoptosis in renal tubular epithelial cells in normal group were found,but more in model group. The expression of CHOP and Caspase-12(P<0.01)were much higher in the model group. Compared with the model group, the number of apoptosis in renal tubular epithelial cells and the expression of CHOP and Caspase-12 were reduced(P<0.05) in Qize decoction low dosage group and Niaoduqing group. Conclusion: Qize decoction low dosage could protect the adenine-induced renal damage, maybe related to that it can inhibit the high expression of ERS-related apoptosis-molecule CHOP and Caspase-12.关键词：Qize decoction;chronic renal failure;endoplasmic reticulum stress;apoptosis;C/EBP-homologous protein;Caspase-12
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发布时间：2024-01-04 - 摘要：Objective: To screen antidepressant activity of baicalin, baicalein, wogonoside and wogonin, the flavonoids from Scutellaria baicalensis. Method: Male ICR mice were randomly divided into 10 groups:control group, fluoxetine group and baicalin, baicalein, wogonoside and wogonin groups. The treatment groups were received four flavonoids at dose of 10, 20 mg·kg-1 and fluoxeine at 20 mg·kg-1 for 7 days respectively. Forced swimming test (FST), tail suspension test (TST) and open field test (OFT) were applied to evaluate antidepressant activity at 1 hour after the last administration. Result: Compared with control groups, the immobility time of FST mice was significantly shorten from (107.6±28.5) s to (64.4±25.1), (73.2±30.8), (69.6±20.4), (79.7±32.6) s, following treatment at dose of 20 mg·kg-1, with baicalin, baicalein, wogonoside and wogonin. Moreover, the immobility time of TST mice was also significantly shorten from (85.2±27.3) s to (43.1±16.4), (52.0±21.1), (48.3±25.9), (62.2±35.8) s, respectively. Particularly, the effects of baicalin and wogonoside were more obvious. In addition, four flavonoids had no effect on spontaneous locomotor activity in mice. Conclusion: Our results suggest that all of four flavonoids compouds from S. baicalensis exhibit antidepressant activity. Moreover, the roles of baicalin and wogonoside were more visible than baicalein and wogonin, which may be relate to the different metabolic processes of these compouds.关键词：depression;Scutellaria baicalensis;flavonoid;forced swimming test;tail suspension test;open field test
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发布时间：2024-01-04 -
Effect of Zhishi Xiebai Guizhi Tang on Hermorrheology and Oxygen Free Radicals in Hyperlipidemia Rat
摘要：Objective: To investigate the effects of Zhishi Xiebai Guizhi Tang on hermorrheology and oxygen free radicala in hyperlipidemia rat. Method: Using hyperlipidemia animal model replicated by hyperlipidemia forage, pravastatin sodium (3.6 mg·kg-1)and different doses of Zhishi Xiebai Guizhi Tang (7.2,3.6, 1.8 g·kg-1) were given to rats in model group. Then indexes of hermorrheology, levels of malondialdenhyde(MDA) and superoxide dismutase(SOD) were assayed. Result: Compared with the control group, the whole blood viscosity at the 200 s-1 and 5 s-1 shear rate and plasma viscosity in the model group were increased, the levels of MDA was increased, the level of SOD was decreased obviously. Compared with model group, the level of the whole blood viscosity at the 200 s-1 and 5 s-1 shear rate and plasma viscosity in Zhishi Xiebai Guizhi Tang(high dose) group and the pravastatin sodium group were decreased, the level of MDA was decreased, the level of SOD was increased. Conclusion: The Zhishi Xiebai Guizhi Tang can improve hermorrheology, clean oxygen free radical in rat with hyperlipidemia.关键词：hyperlipidemia;blood rheology;antioxidatio;Zhishi Xiebai Guizhi Tang- 2
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发布时间：2024-01-04 - 摘要：Objective: The determination conditions and antioxidant activity of polyphenol from Diospyros kaki leaf was studied. Method: Color conditions of polyphenol determination by using Folin-Ciocalteu(FC) colorimetric method were obtained, and anti-oxidation activity of polyphenol from D. kaki leaf and a positive control Vitamin C(VC)were determined. Result: The best color reaction conditions of FC colorimetric method were as follows: Folin-Ciocalteu was 1.5 mL, 75 g·L-1, Na2CO3 was 6 mL, temperature was 25 ℃, reaction time was 60 min,the standard deviation of its accuracy test was 0.51%, the deviation of reproducibility was 1.02%, and the recovery was 96.7%-100.4%.Free radical scavenging ability in vitro was that: HO·>O2 -·>DPPH·. Half maximal inhibitory concentration(IC50)of polyphenol on HO·, O2 -·, DPPH·were 39.33 mg·L-1,0.53, 0.85 g·L-1. And eliminated capacity on HO·and DPPH·went beyond VC,HO·was eliminated by IC50 was only 1/6 of the VC, DPPH·was eliminated by IC50 was 1/3 of the VC. Conclusion: The optimum conditions of determinate polyphenol by using Folin-Ciocalteu method have a well accuracy and repeatability. Polyphenol from D. kaki leaf have strong antioxidation.关键词：Diospyros kaki leaf;polyphenol;determination conditions;antioxidation
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发布时间：2024-01-04 - 摘要：Objective: To explore the immune pathological mechanism of anticardiolipin antibody (ACA) induced fetal loss and the immune regulation mechanism of Anzi Heji. Method: BALB/c mice were randomly divided into 6 groups: control group,model group,low dose group,middle dose group and high dose group of Anzi Heji,aspirin group. At the first day of pregnancy, distilled water was orally given in control group and model group,while the mice in Anziheji groups were given Anziheji groups at dose of 37.7, 75.4, 150.8 g·kg-1·d-1 respectively, the mice in aspirin group were given aspirin with 0.019 5 g·g-1·d-1. The administration was given for 14 days, once a day. ACA-IgG injected to pregnant mice by intraperitoneal injection to establish animal model of ACA induced fetal loss on the day 8 and day 12 of pregnancy. The peripheral blood CD4+CD25+FOXP3+Treg cells ratio was detected by flow cytometry; ACA level of mice pregnancy was detected by ELISA; the embryo resorption and fetal development situation were observed. Result: In model group, the peripheral blood CD4+CD25+FOXP3+Treg cells ratio was significantly lower than the control group(2.67±0.51)% vs (9.85±1.77)%(P<0.01); compared with model group, the low and middle dose group of Anzi Heji could increase CD4+CD25+FOXP3+Treg cells ratio significantly(9.47±1.26)%,(7.61±1.07)% vs (2.67±0.51)%,P<0.01, and significantly reduce the titer of ACA (P<0.01), the low dose group of Anzi Heji was better than the middle dose group of Anzi Heji (P<0.01). The low dose group of Anzi Heji could significantly reduce the resorption rate, and significantly increased fetal weight and placental weight (P<0.05). Conclusion: Pathological mechanism of ACA induced fetal loss is related to decreasing immune cells-CD4+CD25+FOXP3+Treg cells percentage. The immune regulatory mechanism of Anzi Heji on ACA induced fetal loss can be achieved by increasing the CD4+CD25+FOXP3+Treg cells ratio,关键词：anticardiolipin antibody;fetal loss;CD4+CD25+FOXP3+Treg cells;Anzi Heji
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发布时间：2024-01-04 - 摘要：Objective: To study the impact of Yinqin Qinggan decoction on serum nitric oxide(NO), glutathione peroxidase(GSH-Px) in rats with alcoholic liver disease. Method: The rats were fed alcohol and high-fat diet to resultg in alcoholic liver model in rats. The rats were randomly divided into model group, control group, the Chinese medicines (Yinqin Qinggan decocotion), west medicine group 1(tiopronin film), west medicine group 2 (silibinin capsules), with 30 eachgroup respectively.At 4 weeks, 8 weeks and 12 weeks, respectively 10 rats in each group were executed to collect blood,sample and to measure NO, GSH-Px levels. Result: The serum levels of NO in the various stages of model group were significantly higher than the normal group (P<0.01). GSH-Px levels in the various stages was significantly lower than normal (P<0.01). Forth treatment groups in the various stages NO levels were lower than in model group (P<0.05); GSH-Px levels were significantly higher than in model group (P<0.01). Conclusion: Liver Yinqin Qinggan decoction can decrease serum NO levels, increase GSH-Px activity and inhibit of lipid peroxidation, which may be one of the mechanisms.关键词：alcoholic liver disease;Yinqin Qinggan decoction;NO;GSH-Px;peroxidase
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of Hedyotis lance aqueous extract (JYECA) on acute liver injury in mice. Method: The models of carbon tetrachloride (CCl4) and D -GalN-induced acute liver injury in mice with the method of intraperitoneal injection were used to study the protective effects of JYECA. The colorimetric assay was used to measure the activity of alanine aminotransferase (ALT) and aspartate aminotransferase(AST) in blood serum, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver homogenate were determined. Hematoxylin and eosin (HE) stain was used to examine the degree of hepatic injury. Result: JYECA could obviously reduce the activity of AST and ALT and the content of MDA in acute hepatic injury mice, and increase the activity of SOD( P<0.01 or P<0.05). JYECA could reduce the degree of hepatic injury. Conclusion: JYECA has protective effect on acute hepatic injuried mice. The mechanism may be related to attenuating free radical and inhibiting lipid peroxidation.关键词：Hedyotis lance;acute hepatic injury mice;carbon tetrachloride;D-GalN
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发布时间：2024-01-04 - 摘要：Objective: To study the therapeutical effect of Lang Qing A Ta (LQAT) on hepatic fibrosis induced by composite factors in rats. Method: The hepatic fibrosis model was induced by high fat and low protein feed combined with administration of ethyl alcohol and a subcutaneous injection of CCl 4in rats and the model rats were divided into five groups as model group, Fufang Biejia Ruangan table group and LQAT groups at three doses. The rats were orally treated with corresponding decoctions once a day for 7 weeks.The levels of biochemical indicators and hepatic fibrosis indicators in serum were detected, as well as the levels of hydroxyproline, superoxide dismutaso(SOD) and malondialdehyde(MDA) in liver. The histopathologic examination was proceeded to evaluate the pathological changes in livers. Result: LQAT decoction could remarkably decrease the liver coefficients and hydroxyproline contents of liver in hepatic fibrosis rats, decrease the levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT),alkalinephosphatase(ALP), total bilirubin(T-BIL), hyaluronic acid(HA), laminin(LN), procollagen Ⅲ N-terminal peptide(PⅢNP), collagen type Ⅳ(CⅣ) and increase the levels of albumin(ALB) and alburmin/globulin(A/G) in serum. Furthermore, LQAT decoction could obviously increase the activity of SOD and decrease the MDA levels in liver. The results of histopathologic examination showed that LQAT at high and medium doses could ameliorate the pathological changes such as fibroplasia and inflammation and decrease the area of collagen fiber in livers. Conclusion: LQAT decoction has significant therapeutical effects on hepatic fibrosis in rats.关键词：Tibetan medicine;Lang Qing A Ta;hepatic fibrosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effects of ethanol extracts from Lonicera japonic on experimental hepatic fibrosis in rats and its mechanism. Method: The rat hepatic fibrosis model was established with carbon tetrachloride(CCl4,sc 8 weeks,ip 4 weeks).All the experimental rats were divided into four groups: normal group(A),model group(B),colchicines group(C,0.2 mg·kg-1·d-1), ethanol extracts from L. japonic group(D,5.4 g·kg-1·d-1).All groups except A were given carbon tetrachloride(sc 3 mL·kg-1,2/weeks) to induce the rat model of hepatic fibrosis. All experimental rats were killed at eight weeks after the models were established. Serum levels of hyaluronic acid (HA),laminin(LN),pro-collagen type Ⅲ peptide(PCⅢ) and collagen type Ⅳ peptide(CⅣ)were detected by radio immunoassay; and examined the Smad3 mRNA expression in rat hepatic tissue by using RT-PCR technology. The liver histopathological changes were observed with HE or special collegin fibrosis staining and its ultrasructures were observed in electronic-microscope before and after therapy. Result: Compared with normal group in model group,the serum level of LN(207.6±27.4) μg·L-1,PCⅢ(280.1±3.2) μg·L-1,HA(357.0±14.1) μg·L-1), CⅣ(180.0±7.0) μg·L-1, the Smad3 mRNA expression in the rat hepatic tissue were significantly increased (P<0.01),ethanol extracts of L. japonica treatments(8 weeks) significantly decreased the serum level of LN(207.6±27.4) μg·L-1,PCⅢ(280.1±3.2)μg·L-1,HA(357.0±14.1)μg·L-1,CⅣ(180.0±7.0) μg·L-1(P<0.01).Also the Smad3 mRNA expression in the rat hepatic tissue decreased markedly(P<0.01).The liver histopathological improvement was also obvious. Conclusion: L. japonica can effectively suppress the development of hepatic fibrosis, its mechanism may be achieved by down-regulating the Smad3 mRNA expression in hepatic tissue, and reducing the secretion of extracellular matrix(ECM).关键词：ethanol extracts of Lonicera japonica;hepatic fibrosis;extracellular matrix (ECM)
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发布时间：2024-01-04 - 摘要：Objective: To study the pharmacology effect of different polar extracts from Aucklandia lappa and its processed products. Method: The stomach mucous membrane injury model induced by hydrochloric acid-alcohol was adopted. SD rats were divided randomly into control group,model group, A. lappa group(2,6 g·kg-1), processed products of A. lappa group(2,6 g·kg-1), volatile oil group from A. lappa (2,6 g·kg-1), volatile oil of processed products from A. lappa group(2,6 g·kg-1), the gastric percentage was calculated. Result: The essential oil of processed products from A. lappa and A. lappa (2,6 g·kg-1)significantly inhibited the injuries of gastrointestinal mucous membrane(P<0.01), and the activity of essential oil of processed products of A. lappa (6 g·kg-1) was stronger than A. lappa (6 g·kg-1). However, the influence of A. lappa on rats’ gastrin was not remarkable, only essential oil of A. lappa (2g·kg-1) exhibited enhance of rats’ gastrin. Conclusion: The essential oil of A. lappa may hence the inhibition effect on injuriy of stomach mucous membrane.关键词：Aucklandia lappa;processed products;stomach mucous membrane;gastrin
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of Pingweishu capsule on gastric mucosal injury in rats. Method: The rats were divided into control group, model group, Pingweishu capsule low (3.3 g·kg-1),middle(6.6 g·kg-1), high(6.6 g·kg-1) dose groups, Xue-dan-wei-chang pill group (22 g·kg-1) and ranitidine (50 mg·kg-1)group.After giving drugs a week, made the model of acute gastric ulcer in rat by Ethanol, observed the protective effect of Pingweishu Capsule on gastric mucosal injury with high, middle, low dose, and compared the effects with Xuedan- Weichang pill and ranitidine. Used gastrin(GAS) and motilin(MOT)reagent kit to test GAS and MOT content. Result: The stomach tissue lesions in rats was reduced significantly in Pingweishu capsule groups of high and middle dose(Compared with model group, P<0.01). The gastric tissues in high dose group were not significant changes in pathology, just as the normal control group and the group of Xuedan Weichang pill. But in low dose group there was only a little recovery. The GAS and MOT content of Pingweishu capsule groups was higher than that of the model group (Compared with model group, P<0.05 or P<0.01), there was statistically significant difference.Compared with Xuedan Weichang pill group, there was not statistical significance, but high middle dose group of Pingweishu capsule had the trend of increasing the GAS and MTL. Conclusion: Pingweishu capsule could protect the induced gastric mucosal injury in rats by ethanol.
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发布时间：2024-01-04 - 摘要：Objective: To investigate relaxant effect of Compound Danggui decoction(DGD)-containing intestinal absorption on vascular rings of thoracic aorta in rats. Method: The everted gut sac was prepared using intestinal of rats, then was immersed into the crude extractive solution of compound DGD, the K-R solution inside the sac was collected as the intestinal absorption of DGD after 2 hours incubation. The study was performed with the model of rat isolated thoracic aorta rings in organ bath. The effect of accumulated DGD on aorta rings in resting tension, or pre-constricted with KCl, or pre-constricted with phenylephrine (PE) was observed, and were compared with nifedipine. Result: DGD had no significant effects on aorta rings in resting tension or pre-constricted with KCl. When the concentration ration of ferulic acid in DGD was cumulated to 2.48×10-3,4.96×10-3,9.92×10-3 g·L-1, it caused concentration-dependent relaxation while aorta rings were pre-constricted with PE. Nifedipine had no significant effects on aorta rings in resting tension, when the concentration ration of nifedipine was cumulated to 8.66×10-4,17.32×10-4,34.65×10-4 mmol·L-1, it caused concentration-dependent relaxation while aorta rings were pre-constricted with PE, or pre-constricted with KCl. Conclusion: Intestinal absorption of compound DGD can relax the rat thoracic aorta rings with endothelium. The results indicate that intestinal absorption containing drugs may be used to pharmacodynamic study in vitro.关键词：Compound Danggui decoction;drug intestinal absorption;thoracic aorta rings;pharmacodynamic study in vitro
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Naomai Shukang capsule on energy metabolism in rat blood stasis with cerebral ischemia models. Method: Blood stasis model was replicated with injection of dexamethasone. Model rats were administrated orally with Naomai Shukang capsule according to dosages of 0.9,0.6,0.3 g·kg-1 for 10 d. After the rats were administrated with drugs for 1 h on the 11th day, Cerebral ischemia models were made by ligating both sides of the common carotid arteries of rats for 30 min. Na+-K+-ATPase, Mg2+-ATPase, Ca 2+-ATPase activity, lactic acid(LD) content and lactate dehy drogenase(LDH) activity of brain homogenates were measured. Result: Three doses of Naomai Shukang capsule can improve the ATP activities(P<0.01 or P<0.05). The high and medium dose can reduce the LD content(P<0.01). The medium and low dose can increase the LDH activity(P<0.01). Conclusion: Naomai Shukang capsule can improve brain energy metabolism in rat blood stasis with cerebral ischemia models and reduce lactic acid accumulation and damage to brain tissue.关键词：Naomai Shukang Capsule;blood stasis;cerebral ischemia;energy metabolism
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发布时间：2024-01-04 - 摘要：Objective: To study the therapeutic effects of Kangxian capsule, a Chinese patent medicine, on the hepatic fibrosis in rats. Method: Forty male SD rats were randomly divided into normal control group,model group,colchicine group, Kangxian capsule high dose and low dose group. In order to prepare the hepatic fibrosis model, all the rats except those of the normal control group were subcutaneously administrated with the CCL4 olive oil solution for six weeks and were given high-fat diet in the first two weeks. The treatment groups were given different doses of Kangxian capsule and colchicine intervention for six weeks. The control group and model group animals were intragastrically fed with the same volume of normal saline. The activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in rat sera were detected with automatic biochemistry analyzer. The concentrations of hyaluronic(HA), laminin(LN),typeⅢ procollagen (PCⅢ)in sera were measured with the method of radioimmunoassay.The pathohistological changes were observed under microscope. Result: Compared with the model group,the serum levels of ALT,AST,HA,LN,PCⅢ in rats of Kangxian capsule treatment groups were significantly lower(P<0.01).There were much degeneration and necrosis, inflammatory cell infiltration, false lobular formation, and a large number of collagen fiber depositions in liver tissue of the model group. Compared with the model group, the level of chronic hepatic damage was improved greatly and none of the false lobular formed in two dose of Kangxian capsule groups. Conclusion: Kangxian capsule can greatly protect from hepatic fibrosis in rats induced by CCL4.关键词：Kangxian capsule;hepatic fibrosis;rats
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发布时间：2024-01-04 -
Hypotensive Effect of the Alcohol Extract from Gouteng Prescription on Spontaneous Hypertensive Rats
摘要：Objective: To explore the hypotensive effects and mechanisms of the alcohol extract from Gouteng prescription. Method: Thirty spontaneous hypertensive rats (SHR) were randomly divided into five groups,untreated control, the high, middle, low dosage group of the alcohol extract and captopril group, six rats in each group. Tail blood pressure was measured every week. Rats were sacrificed to determine serum(NO),ET,AngⅡ after six weeks of treatment. Result: The alcohol extract from Gouteng prescription significantly lowered the blood pressure of rats, meanwhile significantly increased NO content, decreased markedly the content of ET, but it could not decrease AngⅡ. Conclusion: Alcohol extract from Gouteng prescription had significant hypotensive effect in SHR and its mechamsim may relate to increasing NO content and reducing ET level.关键词：alcohol extract from Gouteng prescription;hypotensive effect;nitric oxide;angiotonin Ⅱ- 2
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发布时间：2024-01-04 - 摘要：Objective: To observe the hypoglycemic effect of Jingshenguo granule on type 2 diabetic rats caused by streptozotocin (STZ). Method: Type 2 diabetic rat model was established by diet of high fat combined with intraperitoneal injection of STZ. And then according to their blood glucose levels, the models were randomly divided into four groups which were model control group, Jingshenguo granule low dosage group (1.0 g·kg-1), Jingshenguo granule high dosage group (3.0 g·kg-1), and metformin hydrochloride group (0.3 g·kg-1). The treatment in each group was administrated ig for fourteen days. The influence from Jingshenguo granule was investigated on type 2 diabetic rats regarding weight, fast blood glucose, total cholesterol, triglyceride, high-density lipoprotein, serum insulin. Result: Low and high doses of Jingshenguo granule could significantly lower (FBG), (TC) and (TG) (P<0.05), and could increase (HDL-C) and (Ins) content (P<0.05) in type 2 diabetic rats after fourteen days’ medical administration compared with that of model control group (P<0.05). And Jingshenguo granule high dose group had better hypoglycemic effect than that of low dose group (P<0.05). Conclusion: Jingshenguo granule can reduce blood glucose and blood lipid in type 2 diabetic rats, which may be related to the increase of insulin sensitivity and regulation of lipids metabolism.关键词：Jingshenguo granule;type 2 diabetes;blood glucose;blood lipids;insulin
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Chinese herbal compound Yitangkang on Nav1.7 protein and mRNA expression in diabetic rats. Method: Forty health male Wistar rats, weighting 200-250 g, were randomly divided into control group, model group and Yitangkang group.The model group and Yitangkang group were given STZ 55 mg·kg-1 ip to induce diabetes model, and respectively given comfort agent and Yitangkang (4 g·kg-1 )5 mL·d-1 ig for 6 weeks, dorsal root ganglia(DRG) specimens were collected to detect Nav1.7 protein and mRNA expression level with immunohistochemistry and RT-PCR method respectively. Result: The blood glucose level in control group was (6.73±0.9) mmol·L-1, in model group being (20.12±1.3) mmol·L-1, in Yitangkang group being (19.34±1.2 )mmol·L-1, which showed a significant differences in model group compared with the control group (P<0.01), no significant difference in Yitangkang group was found compared with the model group. Grayscale values of the Nav1.7 protein expression in blank control group was 149.41± 5.71, in model groups being 104.53± 9.02, in Yitangkang group being 132.57± 6.13. Compared with the control group, the Nav1.7 protein expression in model group was significantly increased (P<0.01), Yitangkang group expression levels significantly lower compared with the model group (P<0.01).Absorbance ratio Nav1.7 mRNA expression of integralin control groups was 0.114± 0.018, in model groups being 0.215± 0.043, in Yitangkang group being 0.128± 0.025. Compared with the control group, the level of expression in model group was significantly increased (P<0.01). The expression levels in Yitangkang group was significantly lowered compared with the model group (P<0.01). Conclusion: Chinese herbal compound Yitangkang may be block the Nav1.7 channel, which may be one of the mechanisms.关键词：diabetes mellitus;neuropathic pain;dorsal root ganglia;Nav1.7;compound Yitangkang
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of artesunate on apoptosis and cell cycle of human colon cancer HCT-8 cells. Method: The experimental groups were negative control group, blank control group, 10, 20 and 30 μmol·L-1 artesunate-treated groups. transmission electron microscope (TEM) and flow cytometry(FCM) were used to analyze the apoptosis of the treated HCT-8 cells by artesunate. FCM was used to analyse cell cycle of the treated HCT-8 cells by artesunate. The levels of Bax and Bcl-2 involved in the different treated HCT-8 cells were detected by Western blot. Result: After treatment of artesunate, it had observed by TEM that the cell shape changed with cytomembrane shrink, karyopycnosis, nuclear fragmentation, and the formation of apoptotic bodies. The rates of apoptosis of 10, 20, 30 μmol·L-1 artesunate-treated groups were 17.1%±3.8%,29.5%±5.1%, 41.4%±5.8%, which was significantly higher than the apoptosis rare of blank control group(5.1%±1.4%, P<0.05). In 20 μmol·L-1 artesunate-treated group, G0/G1 cell proportion was increased with the prolonged drug effects, and S and G2/M cell proportion was decreased. Bax protein expression levels of 10, 20, 30 μmol·L-1 artesunate-treated groups were 0.20±0.03,0.40±0.05, 0.50±0.08, which was significantly higher than that in blank control group(0.06±0.02, P<0.05). The difference of Bcl-2 protein expression levels in the above-mentioned groups was not statistically significant. The protein expression ratio of Bcl-2/Bax showed downward trend. Conclusion: Artesunate can inhibit human colon cancer HCT-8 cell growth and induce apoptosis of HCT-8 cell lines by blocking cell cycle and regulating the expression of Bax genes.关键词：artesunate;HCT-8 cell;cell apoptosis;cell cycle
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Shenxiong Huayu capsule on infracted volume, contents of brain water and the changes of morphological and ultrastructure of acute cerebral ischemia reperfusion in ratsand investigate the protective effect of Shenxiong Huayu capsule on acute cerebral ischemia reperfusion injury in rats. Method: The rats were randomly divided into five groups: sham operation group, ischemia reperfusion group (model group), Shenxiong Huayu capsule high, medium and low dose group(480,240,120 mg·kg-1). Intragastric administration lasted 7 day, once a day. Middle cerebral artery occlusion (MCAO) and reperfushion model was made by an intraluminal filament method. The infracted volume was measured by TTC staining, contents of brain water was measured by dry-wet method and the morphological changes were observed using light and election microscope. Result: Compared with the sham operation group, the infracted volume and contents of brain water were increased in model group and showed a significant difference(all P<0.05); compared with the model group, the infracted volume and contents of brain water were reduced in Shenxiong Huayu capsule dose groups, showed a significant difference(P<0.05, or P<0.01),the high dose group showed most significant actions. Under the light and election microscope, the changes of morphological and ultrastructure of changes were reduced in Shenxiong Huayu capsule groups, and the high dose group showed most significant actions. Conclusion: Shenxiong Huayu capsule may relive acute cerebral ischemia reperfusion injury in rats.关键词：cerebral ischemia reperfusion injury;cerebral edema;Shenxiong Huayu Capsule;rat model
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of Tangbian foot therapy on vibration threshold in patients with diabetes. Method: Sixty patients were randomly divided into treatment group and control group(n=30,each). Both groups were given mecobalamin,and treatment group was additionally given Tangbian foot therapy 2 times a day,for 20 minutes every time, for 4 weeks. Result: The total effective rate was 93.3% and 73.3%(P<0.01). Vbration threshold improved significantly in the treatment group after treatment, which was superior to the control group(P<0.05). Conclusion: The Tangbian foot therapy could not only alleviate the clinical painful symptoms in patients with diabetes, but also improve vibration threshold.关键词：Tangbian;diabetic;peripheral neuropathy;vibration threshold
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Qingfei Xiaoji decoction combined with chemotherapy on quality of life and CYFRA21-1 in advanced lung squamous cell carcinoma patients. Method: A total of 120 patients with advanced lung squamous cell carcinoma were enrolled,68 were randomized to treatment group (chemotherapy plus Qingfei Xiaoji decoction) and 52 to control group (chemotherapy). The reginen of TP/GP was taken for chemotherapy. The variation of tumor marker (CYFRA21-1) in peripheral blood and quality of life before and after therapy with Qingfei Xiaoji decoction were observed. Result: The rising rate of Karnofsky score in treatment group(57.4%) was significantly lower than in control group (38.5%)(P<0.05). The levels of CYFRA21-1 (3.45±3.32) μg·L-1 was significantly lower than before therapy(16.23±13.55) μg·L-1in treatment group (P<0.001).Meanwhile, there was no diffrence in control group(16.98±12.35 vs 13.11±10.45) μg·L-1. Conclusion: Qingfei Xiaoji decoction combined with chemotherapy can improve the quality of life and reduce the level of CYFRA21-1.关键词：Qingfei Xiaoji decoction;lung squamous cell carcinoma;quality of life;tumor marker
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发布时间：2024-01-04 - 摘要：Objective: This article is to analyse the compatible mechanism and the clinical application on Dioscorea opposita and Ophiopogon japonicus and provide some suggestions to highlight the importance of compatibility of medicines in clinical application. Method: Based on the compatible mechanism,combination characters and the modern research, an analysis of the compatible mechanism and the clinical application of D. opposita and O. japonicus was performed. Result: The compatibility of D. opposita and O. japonicus was mainly applied in three clincal aspects such as nourishing Yin,moistening dryness and nourishing the lung. Conclusion: The compatible mechanism is flexible and has a very wide clinical application, which may provide some new thought in clinical application.关键词：Dioscorea opposita.;Ophiopogon japonicus;compatibility;analyzing
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发布时间：2024-01-04 - 摘要：Objective: To observe the curative effect, interleukin-12(IL-12) and tumor necrosis factor-α(TNF-α) change by Yiguanjian plusminus in patients chronic atrophic gastritis, for accumulating the clinical experience, directing the clinical work. Method: Eighty-six patients with chronic atrophic gastritis were divided into two groups. The control group(43 cases) were treated by routine treatment, the observation group(43 cases) were added Yiguanjian plusminus. We observated the efficacy and the change of IL-12 and TNF-α in two groups. Result: The total effective rate was obviously higher in the observation group than in the control group. The expressions of IL-12 and TNF-α were obviously lower in the observation group than in the control group after treatment. Conclusion: The Yiguanjian plusminus chinese medicine can improve the curative effect, regulate the expressions of IL-12 and TNF-α in patients with chronic atrophic gastritis, is worthy of being recommended in clinical treatment.关键词：Chinese medicine;Yiguanjian;chronic atrophic gastritis;IL-12;TNF-α
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发布时间：2024-01-04 - 摘要：Objective: To investigate the changes in the components of erythrocyte membrane proteins of the senile coronary heart disease patients with qi deficiency and blood stasis. Method: Eighteen coronary heart disease patients with Qi deficiency and blood stasis, thirteen healthy old people and ten healthy young people were the coronary patient group and healthy old people control group and healthy young people control group. The composition of erythrocyte membrane proteins was analysed by SDS-PAGE. Result: The percentage composition of band 1 protein, band 3 protein, band 4.1 protein, band 4.2 protein, band 5 protein, band 6 protein and tropomodulin of coronary patients were significantly lower than those of the healthy young people. The percentage composition of band 1 protein, band 3 protein and band 4.2 protein of the healthy old people were significantly higher than those of coronary patients. Conclusion: The mechanism of decreasing antioxidant ability and membrane fluidity may be related with the changes in composition of membrane protein.关键词：coronary heart disease;Qi deficiency and blood stasis;composition of membrane protein;erythrocyte membrane
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发布时间：2024-01-04 - 摘要：Objective: To investigate short-term effect of smoothing the liver and regulating the spleen treatment combined with hyperthermia and intra-arterial infusion chemotherapy for advanced pancreatic cancer. Method: Sixty in-patients with advanced pancreatic cancer meeting the inclusion criteria, were randomly divided into the treatment group with 28 patients(intra-arterial chemotherapy plus intraperitoneal hyperthermia with Sini Decoction) and the control group with 32 patients(intra-arterial chemotherapy alone).Short-term effect, pain score, liver depression and spleen deficiency Syndrome points, spleen deficiency syndrome points, side effects, physical state score were observed. Result: The treatment group CR + PR + SD(85.71%) was higher than that in the control group (71.88%), P<0.05. The treatment group pain control CR + PR + MR(85.71%)was higher than that in the control group(62.5%), P<0.05. Liver depression and spleen deficiency syndrome score in the treatment group and the control group was 10.81±4.09 and 15.27±5.67 accordingly, and showed a statistics difference after intervention treatment than before,P<0.05. Conclusion: Clinical benefit of smoothing the liver and regulating the spleen treatment combined hyperthermia and local intra-arterial infusion chemotherapy is higher than simple arterial infusion chemotherapy.关键词：advanced pancreatic cancer;smoothing the liver and regulating the spleen;intra-arterial infusion chemotherapy;hyperthermia
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发布时间：2024-01-04 - 摘要：Objective: To investigate the changes of T lymphocyte subsets and endothelial function in early diabetic nephropathy and the effect of Bailing capsule on immune intervention. Method: Sixty patients with early diabetic nephropathy were selected and randomly divided into 2 groups, routine group (n=30) was treated with insulin; the Bailing capsule group (n=30) was treated with Bailing capsule(54)combined with insulin. Thirty healthy as control group. After 8 weeks of treatment, the endothelium-dependent flow-mediated dilatation(FMD) of brachial artery was determined with ultrasound,and the levels of soluble intercellular adhesion molecule-1(sICAM-1),endothelin-1(ET-1)and T lymphocyte subsets were measured respectively before and after treatment. Result: All patients with early diabetic nephropathy showed that the CD4+T cells,CD4/CD8,sICAM-1,ET-1 was higher than that of the control group(P<0.05), the CD3+T cells,CD8+T cells and FMD were lower than those of the control group(P<0.05). After treatment the CD4+T cells,CD4/CD8,sICAM-1,ET-1 in the Bailing capsule group[CD4+(37.5.±3.1)%,CD4/CD8(1.56±0.16),sICAM-1(476±94)μg·L-1,ET-1(167±9.9) ng·L-1] was decreased more significantly than routine group [(41.3±2.8)%,(2.07±0.17),(509±89)μg·L-1,(201±13.7) ng·L-1] (P<0.05). After treatment the CD3+T cells,CD8+T cells and FMD in the Bailing capsule group was increased more significantly than routine group (P<0.05). Conclusion: The Bailing capsule can effectively regulate the patient’s cellular immunity and recovery vascular endothelial function with early diabetic nephropathy.关键词：diabetic nephropathies;endothelial dysfunction;T-lymphocyte subsets;Bailing capsule
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发布时间：2024-01-04 - 摘要：Objective: To study effect of Xiaoxun decoction (XXD) with acupoint application in treatment of chronic urticaria. Method: Eighty-two cases of chronic urticaria patients were randomly divided into two groups. Observation group(42 cases)were treated with XXD and acupoint application, control group(40 cases) was treated with Clarityne and famotidine tablets. Thirty days in both groups were one course of treatment, and continuous use of three treatment was applied. Symptom scores, serum IgE levels, differences in efficacy and relapse rate were compared before and after treatment in both groups. Result: Compare with the serum IgE levels differences in efficacy in 30, 60 days of treatment, the two groups had no significant difference.The treatment group was more effective than the control group in the 90 days of treatment(P<0.05). The therapentic effective rates for the treatment group and control group were 64.3% and 37.5% respectively on the 90th day. Relapse rate in observation group was 11.9%, in control group was 45%, the difference was statistically significant(P<0.05). Conclusion: XXD with acupoint application is effective for the treatment of chronic urticaria.关键词：Xiaoxun decoction;acupoint application;chronic urticaria
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发布时间：2024-01-04 - 摘要：Objective: To observe the effects of cordyceps sinensis mycelium combined with enalapril on proteinuria in patients with early diabetic nephropathy. Method: Sixty-eight patients with early diabetic nephropathy were randomly divided into control group treated with enalapriland treatment grouptreated with enalapril and Ccordyceps Sinensis Mycelium,each group was treated for 12 weeks. The urinary albumin ejection rate(UAER), 24-hour urine protein,urine α1-microglobin(α1-MG),β2-microglobin(β2-MG) and glycosylated hemoglobin(HbAlc) were measured before and after the treatment. Result: After treatment UAER, 24-hour urine protein,α1-MG,β2-MG in both groups were significantly decreased compared with that before treatment(P<0.05)and the difference in combination group was significantly greater than that in single medication group(P<0.05).The difference of HbA1c before and after treatment had no statistical significance in both groups. Conclusion: The treatment with both cordyceps sinensis mycelium and enalapril can decrease proteinuria of Patients with early diabetic nephropathy markedly, and is better than the treatment with enalapril only.关键词：Cordyceps Sinensis Mycelium;enalapril;early diabetic nephropathy;urinary albumin ejection rate
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发布时间：2024-01-04 - 摘要：The literature, in recent years, related to IPK in pharmacological and toxicological studies was compiled and analyzed.One of the peculiarities of its method is that the perfusion fluid may be pulled repeatedly from ureter and renal vein for dynamically detecting the change of xenobiotics and their metabolin in kidney in the short run.Therefore, the method may be applied to study the drug excretion, mechanism of action and drug combination etc. Furthermore, by determining the change of protein activity and content in perfusion fluid, combined with histopathological observation, the method may be used to study the characteristic,extent and mechanism of drug induced renal injury.This article summarized the characteristic of IPK and its application into the pharmacological and toxicological studies in order to provide reference for scientific research and clinical application of the drugs.关键词：isolated perfused kidney;pharmacology;toxicology
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发布时间：2024-01-04 - 摘要：To make the analysis of literature about flavescens and its active ingredient in the treatment of insomnia. Search terms were flavescens, matrine, oxymatrine, insomnia. The full text journal database (CNKI) literature of the past ten years were retrieved,and then to select and reference literature from the pathology and treatment of insomnia medication, as well as the sophora flavescens and its active ingredients mechanism.Forty literatures were quoted among four hundred and fifty-two items. Material basis and the homeostatic regulation of sleep imbalances are the main pathological cause of insomnia. Flavescens and its active ingredients can increase the brain of a sedative neurotransmitters, and then perform the effect on insomnia. Flavescens and its active ingredient could pass through the blood-brain barrier, regulate neurotransmitters in the brain and result in central sedation, and then to perform the effect on insomnia. In further studies,flavescens and its related agents should be developed and application.关键词：flavescens;matrine;oxymatrine;insomnia;centre calm
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发布时间：2024-01-04 - 摘要：To summarize the bioactive constituents in Salvia miltiorrhizae radix et rhizoma and its latest isolation and analysis methods and to come to a better understanding on the species and provide beneficial support for its further development. Based on the related literatures, the main constituents and their bioactivity were summarized, as well as their latest isolation and analysis methods. Studies indicated the main constituents were phenolic acids and diterpene quinones with various bioactivity, which were the material foundation for promoting blood circulation and relieving blood stasis, and inducing menstruation to stop pain, etc. Additionally, there were some new isolation and analysis methods for the above bioactive constituents.关键词：Salvia Miltiorrhizae Radix et Rhizoma;bioactive constituents;diterpene quinone;phenolic acid;isolation;analysis
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发布时间：2024-01-04 - 摘要：The present paper reviews the chemical compounds, the biological activities and clinical application of Androsace plants, which would provide references for development and utilization of Androsac genus. Index the literatures in CNKI,PubMed and Science Direct direct databases choosing titles or keywords as 'Androsace’. Androsace is an important genus of the family Primulaceae. About 100 species distribute in many zones of the world. Up to now, more than 30 compounds have been isolated and identified, and the flavonoids and triterpenoid saponins are the main constitutent. In vivo and in vitro studies indicated that rude extracts and monomer compounds (triterpenoid saponins) from genus Androsace possessed wide pharmacological actions, especially in anti-tumor, anti-early pregnancy and anti-viruses, etc.关键词：Androsace;flavonoids;triterpenoid saponins;anti-tumor
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发布时间：2024-01-04 - 摘要：To standardize behavior of diagnosis and treatment by traditional Chinese medicine for blood side effects resulted from accquired immure dieficiency syndrome(AIDS) highly active antiretroviraltherapy(HAART) drugs, and establish their diagnosis and treatment procedures and evaluate preliminarily. The literatures at home and abroad were reviewed and based on suggestions of experts, the questionnaire of Chinese medicine diagnosis and treatment procedures in AIDS HAART blood toxicity was surveyed through 2 bouts of demonstration. The procedures on diagnosis and treatment by Chinese medicine for blood side effects resulted by related to AIDS HAARTdrugs were established. Preliminarily standardization on the concept, etiology, diagnosis, syndrome and treatment by Chinese medicine in AIDS HAART blood toxicity was formed and received by experts.关键词：AIDS;HAART;highly active antiretroviraltherapy blood side effects;Chinese medicine diagnosis and procedures;survey questionaires
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发布时间：2024-01-04 - 摘要：To provide research ideas and theoretical basis for relevant researches through summarizing on evaluation methods of pulmonary inhalation either in vivo or in vitro. Classifying various evaluation methods of pulmonary inhalation according to their characteristics after collecting, analyzing and concluding related 27 articles in last decade from the databases of CNKI, PubMed, ScienceDirect. Predominant evaluation methods were listed as follows:cascade impactor method, modern optic instruments method, pharmacodynamic evaluation method, pharmacokinetic evaluation method, intrapulmonary deposition method and so on. Currently, there were many problems yet to be solved for evaluation methods of pulmonary inhalation, for example, correlation between in vivo and in vitro evaluation methods was low, methods were single of pharmacodynamic and pharmacokinetic evaluation, animal models were not conforming to real situation of human body and safety assessment system was imperfect.All of these should be gradually improved and perfected in the future studies.关键词：pulmonary delivery;inhalation preparation;in vivo and in vitro evaluation
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发布时间：2024-01-04 - 摘要：Animal models with deficiency syndrome included four types like Qi deficiency, blood deficiency, Yang deficiency and Yin deficiency. Under direction of the strengthening the body resistance theory of traditional Chinese medicine(TCM) the research work on interfering effects of herbs on animal models with deficiency syndrome plays an important role. Up to now, forty kinds of animal models related to syndrome and sign of TCM have been established, which have brought a wide application in the research work. But,on the other hand, some limitations and disadvantages existed. The key point lies in whether the animal models concerning syndrome and sign of TCM can be consistent with clinical symptoms. The interfering effects of Chinese herbs with strengthening the body resistance functions need be comprehensively evaluated in at lest two kinds of the animal model. In order to understand current status about animal models with deficiency syndrome and to provide reference for researchers of TCM area, modeling methods of animal model with deficiency syndrome, observation indexes and application of Chinese herbs in the models were reviewed.关键词：Fuzheng Guben principle;Qi deficency;blood deficency;Yang deficency;Yin deficency;animal model
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发布时间：2024-01-04 - 摘要：Caesalpinia minax is one of common medicinal plant for Guangxi, it is used to treatment of influenza and herpes zoster. This paper reviews herbal textual research and literature on chemical constituents, pharmacological action. Recorded in C. minax Hance books, as well as domestic and overseas literature reports to analyze and conclude. C. minax Hance of seeds, stems, roots, tender shoots have similar efficacy, the seeds have a significant anti-influenza active ingredient for the furan diterpenoids composition, and most of the new compounds found in nature for the first time.There has been a great research achievement on C. minax Hance of seeds. But the drug source, it advises to research the plant of other medicinal site, and expand the drug source to provide further reference for future reasonable development of the plant’s medicinal value.关键词：Caesalpinia minax;Semen Caesalpiniae;pharmacological action
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发布时间：2024-01-04
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