最新刊期
卷 19 ， 期 19 ， 2013
- 摘要：Objective: To prepare curcumin conjugated O-carboxymethyl chitosan nanoparticles(Cur-OMCS) and investigate its ability to inhibit HepG2 cells. Method: Curcumin was conjugated to O-carboxymethyl chitosan by esterification reaction. Cur-OMCS was measured by UV-Vis,IR,NMR and TEM.Anticancer activity of Cur-OMCS was investigated by MTT. Result: Cur-OMCS could form nanoparticles by self-assembly in aqueous media with average particle size of 319 nm,particle size distribution index of 0.488,Zeta potential of -26.1 mV and drug loading of 1.5%. The solution showed clear and transparent with yellow.The concentration of curcumin in aqueous media increased 300 times with critical micelle concentration of 0.040 g·L-1. The inhibitory effect on HepG2 cells of Cur-OMCS was much better than that of free curcumin with the cell proliferation inhibition rate of 80% when the concentration of curcumin reached 15 mg·L-1. Conclusion: Self-assembled nanoparticles of curcumin conjugated O-carboxymethyl chitosan increased anticancer activity of curcumin.关键词：curcumin;O-carboxymethyl chitosan;self-assemble;nanoparticles;HepG2 cells;critical micelle concentration
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发布时间：2024-01-04 - 摘要：Objective: To investigate effects of different boiling conditions on quality of Buyang Huanwu decoction by double decocting non-pressurized machine. Method: A single factor test was adopted to investigate levels of adding water,with the contents of total solids,ferulic acid and astragaloside as comprehensive evaluation index,effects of soaking time,the amount of water,boiling times and time on technology were investigated by orthogonal test.The contents of ferulic acid and astragaloside were determined by HPLC. Result: Analysis of variance showed that effect of factors on boiling technology was in order of decoction times>the amount of water>decoction time>soaking time,only decoction times had significant impact.Optimum non-pressurized boiling technology was as following:soaked 30 min with 8-10 times the amount of water,decoction for 2 times,60 min per time. Conclusion: Optimized technology was stable and feasible,it provided theoretical basis for ferulic acid and astragaloside as main ingredients in traditional Chinese medicine decoctions.关键词：Buyang Huanwu Decoction;atmospheric pressure decoction machine;technology optimization;orthogonal test;HPLC;ferulic acid;astragaloside
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发布时间：2024-01-04 - 摘要：Objective: To determine equilibrium solubility and apparent oil/water partition coefficient of artesunate. Method: Equilibrium solubility of artesunate in water,different organic solvents and some mixed solvent systems were determined by HPLC,chromatographic conditions were as follows:Diamonsil C18(2) column (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-phosphate buffer (60:40),flow rate 1.0 mL·min-1,detection wavelength at 210 nm,column temperature 30℃.Oil/water partition coefficient of artesunate in n-octanol-water/phosphate buffer solution system was established by shake-flask method. Result: Equilibrium solubility of artesunate in water and other organic solvents(chloroform,acetone and absolute ethyl alcohol) was (0.36±0.016),(598.74±13.11),(284.63±7.96),(186.03±8.27) g·L-1 at 32.0℃.Oil/water partition coefficient of artesunate in n-octanol-water system was (249.03±3.76) with logPapp=(2.40±0.006 5). Conclusion: Artesunate had strong fat-soluble,pH value had a greater impact for its equilibrium solubility and apparent oil/water partition coefficient.Equilibrium solubility of artesunate increased when increasing pH value within 5.50-7.43,apparent oil/water partition coefficient of artesunate decreased when pH value increased,but logPapp remained>2.0,indicating that artesunate had strong membrane permeability in in vitro and in vivo circumstances.关键词：artesunate;equilibrium solubility;apparent oil/water partition coefficient;HPLC;shake-flask method
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology of salvianolic acid B by Zn2+ precipitation method. Method: HPLC was adopted to determine the content of salvianolic acid B,chromatographic conditions were as follows:mobile phase of acetonitrile-0.05% phosphoric acid gradient elution,column temperature 30℃,detection wavelength 286 nm,injection volume 10 μL.With yile of salvianolic acid B as index,extraction technology was optimized by single factor tests;With transfer rate of salvianolic acid B as index,single factor tests were adopted to optimize purification technology by taking alkali liquor species,dosage of ZnCl2,the molar concentration of HCl and pH as factors. Result: Optimized purification technology of salvianolic acid B was as following:soaked 3 times with 6-fold the amount of pH 2-3 HCl,1 h each time,adjusted pH to 6.0-6.5 by 1%Na2CO3,dosage of ZnCl230% as quality of medicinal materials,dissolved precipitate with 2 mol·L-1HCl,adjusted pH to 2-3,extracted 2 times with the same times the amount of ethyl acetate,dried it with vacuum and low temperature,yield of salvianolic acid B 3.47%,purity of 80%. Conclusion: Optimized technology was stable and feasible,it could avoid damage of salvianolic acid B under prolonged high temperature and pH conditions.关键词：salvianolic acid B;Zn2+;precipitation;purification;HPLC;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To compare in vitro dissolution of geniposide from ultrafine and ordinary powder of mongolian Gardeniae Fructus. Method: The content of geniposide was determined by HPLC,chromatographic conditions were as follows:Elite C18 column(4.6 mm×250 mm,5 μm),detection wavelength 238 nm,mobile phase of acetonitrile-water (15:85),flow rate 1.0 mL·min-1,injection volume 20 μL.Dissolution amount and dissolution rate of geniposide from ultrafine and ordinary powder of mongolian Gardeniae Fructus were determined,and in vitro dissolution behavior was analyzed. Result: Dissolution amount of geniposide from ultrafine and ordinary powder of mongolian Gardeniae Fructus were 34.019 and 52.638 mg·g-1,in vitro dissolution curves were in line with Weibull distribution,t50,td,t70,t80 of ordinary powder were 1.501,14.211,70.877 and 2 339.955 min,respectively,but them of ultrafine powder were 1.339,1.505,1.637,1.964 min.Dissolution rate of geniposide from ultrafine powder was higher than common powder,the largest cumulative dissolution of ultrafine powder was raised 30% by comparing with common powder. Conclusion: Superfine grinding could promote dissolution of geniposide in mongolian Gardeniae Fructus,it revealed that application of superfine grinding to deal with mongolian Gardeniae Fructus was feasible.关键词：mongolian Gardeniae Fructus;ultrafine powder;in vitro dissolution;geniposide
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发布时间：2024-01-04 - 摘要：Objective: To investigate content changes of main active constituents in Aurantii Fructus before and after frying with wheat bran. Method: The contents of naringin,neohesperidin,synephrine,nobiletin,tangeretin,meranzin hydrate,meranzin,marmin and auraptene were determined by RP-HPLC,content changes of main active constituents from ten batches of Aurantii Fructus before and after frying with wheat bran were investigated. Result: The contents of naringin,neohesperidin,synephrine,nobiletin,tangeretin,meranzin hydrate,meranzin and marmin fell down after Aurantii Fructus fried with wheat bran,but the content of auraptene increased significantly.Small dose(0.6 mg·kg-1) of auraptene had role in promoting normal mice intestinal motility,while high dose(9 mg·kg-1) showed inhibition. Conclusion: Increasing of the content of auraptene risen was purpose of Aurantii Fructus processed with wheat bran,it could elaborate processing mechanisms of Aurantii Fructus through analyzing variation of main active ingredients.关键词：Aurantii Fructus;fried with wheat bran;main active constituents;RP-HPLC;efficacy trials;content change
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发布时间：2024-01-04 - 摘要：Objective: To establish an RP-HPLC method for determination of pulsatilla saponin B3 and pulsatilla saponin BD in pulsatilla total saponins emulsions,and determine its in vitro release. Method: Liquid-liquid extraction method was adopted to dispose samples and HPLC was adopted to detect.Chromatographic conditions were as follows:Diamonsil C18 column (4.6 mm×250 mm,5 μm),C18 guard column (4.0 mm×3.0 mm),column temperature 40℃,mobile phase of acetonitrile-water-tetrahydrofuran (52:45:3),flow rate 1.0 mL·min-1,detection wavelength 203 nm,injection volume 10 μL.In vitro release tests of pulsatilla total saponins emulsions and pulsatilla total saponins solution were adopted modified dynamic dialysis. Result: Linear ranges of pulsatilla saponin B3 and pulsatilla saponin BD were 0.01-1(r=0.999 6) and 0.01-2(r=0.999 8) g·L-1,RSD of precision were 3.19% and 1.17%,RSD of repetition were 1.40% and 1.60%,average recoveries were 99.63% and 100.27%.The concentration of pulsatilla saponin B3 in three batches of pulsatilla total saponins emulsions were 0.186,0.190,0.191 g·L-1,the concentration of pulsatilla saponin BD were 0.069,0.065,0.066 g·L-1,respectively.Cumulative release of pulsatilla saponin B3 and pulsatilla saponin BD from emulsions in pH 7.4 phosphate buffer reached 91.30% and 74.97% within 12 h,respectively. Conclusion: This established method was accurate,sensitive and suitable for determination of index components and release of Pulsatillae Radix emulsions.关键词：total saponins from Pulsatillae Radix;liquid-liquid extraction;HPLC;emulsions;release
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发布时间：2024-01-04 - 摘要：Objective: To compare quality differences of compound Danshen decoction(CDD) prepared by three different methods,such as traditional marmite,high-pressure decocting machine and double decocting non-pressurized machine. Method: CDD was prepared by traditional marmite,high-pressure decocting machine and double decocting non-pressurized machine,respectively.The content of salvianolic acid B in decoction was determined by HPLC,chromatographic conditions were as follows:LunaC18 column(4.6 mm×250 mm,5 μL),mobile phase of acetonitrile-methanol-formic acid-water(10:30:1:59),detection wavelength of 286 nm,flow rate 1.0 mL·min-1,injection volume 10 μL. Result: The contents of salvianolic acid B in compound Danshen decoction prepared by traditional marmite,high-pressure decocting machine and double decocting non-pressurized machine were 2.534,2.673,2.766 g·L-1 with RSD of 3.32%,1.15%,1.25%(n=5),respectively. Conclusion: Double decocting non-pressurized machine was more suitable for preparation of compound Danshen decoction than the other two methods.关键词：traditional boiling method;high-pressure decocting machine;double decocting non-pressurized machine;compound Danshen decoction;salvianolic acid B;determination
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total saponins and total flavonoids from Anredera cordifolia. Method: The contents of total saponins and total flavonoids were determined by UV.With extraction amounts of total saponins and total flavonoids as indexes,extraction times was investigated by single factor test,effects of ethanol concentration,extraction time and ethanol amount on extraction technology of total saponins and total flavonoids were investigated by orthogonal design. Result: The best extraction conditions for total saponins and total flavonoids from A. cordifolia was as following:extracted 2 times with 12-fold the amount of 75% ethanol,2 h per time;Average extraction volume of total saponins and total flavonoids were 16.23,46.40 mg·g-1,respectively. Conclusion: Optimized extraction process was reasonable and feasible.关键词：Anredera cordifolia;total saponins;total flavonoids;extraction technology
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发布时间：2024-01-04 - 摘要：Objective: To optimize liquid fermentative conditions of intracellular polysaccharides from Polyporus umbellatus. Method: Fermentative medium for intracellular polysaccharides in P. umbellatus was prepared by shake flask cultivation.With yield of intracellular polysaccharides from P. umbellatus as index,nitrogen source,carbon source and fermentation conditions were investigated by single factor tests,orthogonal test was adopted to optimize composition of culture medium. Result: The best carbon sources was saccharose,optimum nitrogen source was soybean meal,optimized composition of culture medium was as following:potato 10.0%,saccharose 4.0%,soybean meal 0.3%,KH2PO4 0.2%,MgSO4·7H2O 0.15%;Fermentation conditions were:initial pH 5.2-5.6,shaking speed 180-200 r·min-1,culture temperature 28℃,culture time 7 d. Conclusion: It had advantages in fermentation period and polysaccharide production by liquid fermentation,meanwhile it was important to reduce costs and industrial production of polysaccharides from P. umbellatus.关键词：Polyporus umbellatus;liquid fermentation;intracellular polysaccharides;orthogonal test;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To optimize streamed processing technology of Dendrobii Caulis with wine by central composite design-response surface methodology. Method: The content of dendrobine was determined by HPLC,chromatographic conditions were as follows:Diamonsil C18 column (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(0.1% triethylamine) gradient elution,flow rate 1 mL·min-1,detection wavelength 240 nm,column temperature 30℃,injection volume 10 μL.The content of polysaccharides was determined by UV.With the concentration and amount of wine,steaming time as independent variables,overall normalized value of the contents of dendrobine and polysaccharides as dependent variable,processing technology was optimized by central composite design-response Surface methodology. Result: Linear range of dendrobine was 0.336-3.36 μg(R2=0.999 8),RSD of precision was 1.24%,RSD of repeatability was 1.06%,average recovery was 97.62%(RSD 1.45%);Linear range of polysaccharides was 0.020 32-0.101 6 g·L-1(R2=0.997 1),RSD of precision was 1.71%,RSD of repeatability was 1.90%.Optimum processing conditions were:the concentration of wine 48%,the amount of wine 17%and steaming time 6.78 h.Deviation between the predicted value(0.292%) and the measured value(0.303%) of the mass fraction of dendrobine was 3.76%,while deviation between the predicted value(11.8%) and the measured value(12.3%) of polysaccharides was 4.24%,established model had a good prediction. Conclusion: Optimized processing technology was simple and stable.关键词：Dendrobii Caulis;dendrobine;polysaccharides from Dendrobii Caulis;processing technology;HPLC;central composite design-response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction and purification processes of sedative and anti-fatigue ingredients from Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis. Method: The contents of sedative and anti-fatigue ingredients were determined by HPLC,chromatographic conditions were as follows:TechMate C18 column(4.6 mm×250 mm,5 μm),detection wavelengths of syringin,acanthopanax E,isofraxidin at 265,209,344 nm,flow rate 1.0 mL·min-1,column temperature 30℃,injection volume 10 μL,mobile phase of acetonitrile-0.1% formic acid gradient elution.With the contents of syringin,eleutheroside E,isofraxidin as comprehensive evaluation index,orthogonal test was adopted to optimize extraction technology with ethanol concentration, extraction times and ethanol amount as factors,types of macroporous resin was screened by static adsorption-elution test,purification technology of sedative and anti-fatigue ingredients from Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis was optimized by single factor tests. Result: Optimum extraction process was as following:extracted 3 times with 10-fold the amount of 70% ethanol,1 h each time.D101 macroporous resin was selected,optimum purification technology was:sample volume 1:1.3-1:1.4,the concentration of sample solution was 0.33 g·mL-1,eluted with 3.5 BV 30% ethanol,purify of sedative and anti-fatigue ingredients 18.35%. Conclusion: Optimized extraction technology was reliable and repeatable,yield of active ingredients of Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis had satisfactory results.关键词：eleutheroside E;syringin;isofraxidin;separation and purification technology;determination
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发布时间：2024-01-04 - 摘要：Objective: To optimize submerged fermentation technology of Massa Medicata Fermentata. Method: With activity of amylase and protease as indexes,single factor test was adopted to investigate effects of flour-wheat bran ratio and different inorganic salts on fermentation technology;With activity of protease as the index,orthogonal test was used to optimize submerged fermentation technology of Massa Medicata Fermentata with fermentation temperature,shaking speed,ratio of flour-wheat bran and the amount of inorganic salts as factors. Result: Aspergillus sydowii and K2HPO4 were selected,optimum submerged fermentation technology was as following:added 12 g flour-wheat bran(1:6) and 0.15 g K2HPO4 into 150 mL water,fermentation temperature at 28℃,shaking speed 180 r·min-1. Conclusion: This optimized fermentation technology was stable and feasible,it could provide a reference for fermentation processing technology of Chinese materia medica.关键词：Massa Medicata Fermentata;aspergillus sydowii;activity of enzyme;orthogonal test;amylase;protease;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To compare differences of efficacy against hepatic injury and contents of major ingredients among Americana Linn extracts prepared by different processes. Method: With water and ethanol as solvents,Americana Linn was extracted by reflux method,smashing method and magnetic stirring method,respectively.Efficacy was tested with acute hepar-injured mice model caused by CCl4,the contents of total peptides and free-amino acid were determined by Bradford and Ninhydrin colorimetric method,combining with cream rate,differences among processes was investigated by calculating weighted score. Result: Water smashing-extract could obviously resist increasing of MDA,ALT and AST in serum and relieve pathological changes of acute hepar-injured mice caused by CCl4.In each extracts,cream rates were 16.64%,20.84%,16.19%,15.75%,19.53% and 16.82%,respectively;The contents of total peptides were 0.82%,0.03%,0.96%,0.02%,0.64%,0.02%,respectively;The contents of free amino acid were 1.99%,1.11%,2.12%,1.15%,2.10%,1.03%,respectively;Composite scores were 90.64,37.31,99.46,42.53,82.42,39.00,respectively. Conclusion: Water smashing-extract of Americana Linn had a good efficacy against hepatic injury and a high score of technic indexes.关键词：Americana Linn;smashing extraction method;total peptides;free-amino acid;hepatic injury
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for simultaneous determination of salviamolic acid B,tanshinoneⅡA and ferulic acid from Lemai dispersible tablets,then investigate in vitro dissolution characteristics of these three ingredients. Method: HPLC was adopted to determine the contents of salviamolic acid B,tanshinoneⅡA and ferulic acid,chromatographic conditions were as follows:AlltimaTM ODS C18 column(4.6 mm×150 mm,5 μm),mobile phase of acetonitrile-0.5% acetic acid gradient elution,flow rate 1.0 mL·min-1,detection wavelength of 320(0-10 min),286(10-20 min),270(20-30 min) nm,column temperature at 30℃,injection volume 20 μL.In vitro dissolution was determined by small cup method,with 0.1 mol·L-1HCl as dissolution medium,rotate speed was 50 r·min-1and dissolving time was 60 min,accumulative dissolution of salviamolic acid B,tanshinoneⅡA and ferulic acid within different times were calculated,resemblance of release curves were compared with similarity factors(f2) method. Result: Linear range of salviamolic acid B,tanshinoneⅡA and ferulic acid were 30.15-301.48,0.15-1.52 and 0.66-6.55 mg·L-1,respectively.Compared with salviamolic acid B,f2 of tanshinoneⅡA and ferulic acid were 70.35 and 82.49,respectively. Conclusion: Dissolution of salviamolic acid B,tanshinoneⅡA had similar characteristics with ferulic acid in Lemai dispersible tablets.关键词：Lemai dispersible tablets;salviamolic acid B;Tanshinone ⅡA;ferulic acid;dissolution;similarity factor
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发布时间：2024-01-04 - 摘要：Objective: To optimize preparation technology of pulsatilla total saponins colon-targeted pellets. Method: HPLC was adopted to determined the content of total saponins from Pulsatillae Radix,chromatographic conditions were as follows:Hypersil ODS-C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.1% phosphoric acid solution (39:61),flow rate 1.0 mL·min-1,column temperature 30℃,detection wavelength 203 nm,injection volume 20 μL.Pellets core was prepared by extrusion spheronization method,with pellets yield,roundness,bulk density,friability as comprehensive evaluation index,orthogonal test was adopted to optimize preparation technology of pulsatilla total saponins colon-targeted pellets;With cumulative release rate as index,effects of plasticizer amount,anti-adhesion agent dosage and coating weight on coating process were investigated by single factor tests. Result: The best preparation technology of pellets core was as following:dosage of MCC 60%,mannitol amount 10%,extrusion frequency 20 Hz,rolling frequency 45 Hz,rolling time 5-8 min;Optimum coating process was:with Eudragit S100 as coating material,15% triethyl citrate as plasiticizer,50% talcum powder as anti-adhesion agent,coating weight of 20%. Conclusion: Optimized process was stable and feasible,prepared pellets had good molding and colon-targeted effect.关键词：Pulsatillae Radix;colon-targeted pellets;orthogonal test;fluidized bed coating;single factor test
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation technology of Angelicae Dahuricae Radix coumarin liposomes and investigate its release properties. Method: Liposomes of coumarin from Angelicae dahuricae Radix were prepared by film dispersion-supersonic method,based on single factor tests,with encapsulation efficiency and drug loading as comprehensive evaluation index,Box-Behnken design-response surface method was adopted to optimize formulation technology with proportion of phosphatidylcholine to cholesterin,ratio of phosphatidylcholine to coumarin and ultrasonic time as factors.The content of coumarin was determined by UV spectrophotometry,appearances and particle size of coumarin liposomes were examined with transmission electron microscopy. Result: Optimum formulation technology was as following:proportion of phosphatidylcholine to cholesterin (6:1),proportion of phosphatidylcholine to coumarin (5:1),ultrasonic time 34 min;Prepared coumarin liposomes had uniform size,showed spherical vesicles with good dispersion,particle size was (112.08±1.21) nm,encapsulation efficiency was (94.02-1.56)%,drug loading was (5.42±0.35)%.Cumulative release of coumarin was 100% in 3 h,cumulative release of mixed solution of coumarin and blank liposomes was 95.3% within 12 h,but that of Angelicae dahuricae Radix coumarin liposomes was 45.6% in 12 h. Conclusion: This optimized process was stable and suitable for industrial production,preparedcoumarin liposomes had high encapsulation efficiency and significant release effects with uniform morphology and particle size.关键词：coumarin from Angelicae Dahuricae Radix;liposomes;preparation technology;Box-Behnken design;response surface methodology;in vitro release
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation technology of matrine solid lipid nanoparticles and investigate its appearance and particle size distribution. Method: Matrine solid lipid nanoparticles were prepared ultrasonic dispersion method,with entrapment efficiency of matrine as index,orthogonal test was adopted to investigate effects of ratio of matrine-stearic acid,ratio of stearic acid-soybean lecithin,poloxamer concentration and oil-water phase ratio on formulation technology. Result: Optimum formulation technology was as following:matrine-stearic acid (1:6),stearic acid-soybean lecithin (1:1),poloxamer concentration 12 g·L-1,oil phase-water phase (1:5).Prepared matrine solid lipid nanoparticles was emulsion solution with luster,showed ivory white,it may produce white flocculus after shaking,average encapsulation efficiency was 82.8%,average drug loading was 5.5%.These nanoparticles were irregular spherical or ellipsoidal with uniform distribution,average particle size was 155 nm. Conclusion: Optimzed formulation technology was feasible and stable with good application prospects.关键词：matrine;solid lipid nanoparticles;formulation technology;orthogonal test;particle size;encapsulation efficiency
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发布时间：2024-01-04 - 摘要：Objective: To study and analyze the content of sialic acid (SA) among different edible bird's nest (EBN), so as to provide reference for EBN quality standard. Method: Using HPLC to detect the content of SA·2HCl is the derivatization reagent; the mobile phase consisted of acetonitrile-water(15:85); the flow rate was 1.0 mL·min-1]. Result: The water content in 25 kinds of EBN ranged from 9.13% to 17.58%,the SA content in 25 kinds of EBN ranged from 3.62% to 15.23% respectively. The content of SA in tremella and pigskin was found zero while using this method. Conclusion: The method is simple, reliable and precise, which could be used to identify the EBN, tremella and pigskin. Althought there's no difference among white, red and yellow EBN, the data could provide support for the further study on the quality of EBN.关键词：edible bird’s nest;sialic acid;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To determine the concentration of trace elements, so as to provide information for the qualitative control of Cordyceps sinensis fermented mycelium, which is more strictly than the standard of Chinese Pharmacopoeia. Method: Flame atomic absorption technique was applied to analyze the content of zinc, iron, manganese, copper and plumbum from fermented mycelium of Cordyceps sinensis respectively. Result: The content of trace elements was listed here in order of relative quantity, Fe>Mn>Zn>Cu>Pb. All calibration curves showed good linearity (r>0.9994). The RSD of reproducibility was between 0.54% and 0.86%. The average recoveries were between 96.05%-101.71%. The RSD of recovery was between 0.32% and 1.91%. Conclusion: The method is simple with good precision and sensitivity and the result was reliable. The content of trace elements in fermented mycelium of C. sinensis is according to the requirement given by the WHO.关键词：Cordyceps sinensis fermented mycelium;trace elements;flame atomic absorption spectrometry
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发布时间：2024-01-04 - 摘要：Objective: The secondary metabolites of marine fungus Aspergillus aculeatus CGD12 and their antibacterial activities were investigated. Method: Column chromatography on silica gel, ODS, Sephadex LH-20 and semipreparative HPLC were used to isolate and purify the compounds from EtOAc extract of the fermentation broth. Their structures were elucidated by NMR,MS spectroscopics data analyses and compared with the data of reported literatures. In order to evaluate the antibacterial activities of these compounds, microdilution broth method was adopted. Result: Seven compounds were isolated from the fermentation broth of A. aculeatus CGD12 and identified as versiol (1),JBIR74(2), JBIR75(3), cyclo-(L-Pro-L-Tyr)(4), cyclo-(L-Pro-L-Val)(5),cyclo-(L-Pro-L-Ile)(6),cyclo-(L-Ala-L-Leu)(7). Conclusion: Compounds 4 and 7 show antibacterial activities against Staphyloccocus aureus and S. epidermidis. The weak antibacterial activities which against Pseudomonas aeruginosa, Klebsiellar pneumonia and Escherichia coli of compound 3 are reported for the first time.关键词：mangrove;Aspergillus aculeatus;metabolites;antibacterial
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents of Spiranthes sinensis. Method: The constituents of the EtOAc-soluble portion of 95% ethanol extract were isolated and purified by means of chromatography. Compounds were identified by their physical characteristics and spectral features. Result: Five compounds were isolated and identified as 3(-hydroxy-urs-12-en-28-oicacid (1), 5-hydroxyl-7,3,4'-timethoxy-flavone (2), sinetirucallol (3), daucosterol (4), and β-sitosterol (5). Conclusion: Compound 1 was first seperated from orchidaceae and compounds 2 was isolated from this plant for the first time.关键词：Spiranthes;Spiranthes sinensis;chemical constituent;spectroanalysis
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发布时间：2024-01-04 - 摘要：Objective: To determine and analyze the ginsenosides contents of Panax quinquefolium which cultivated in farmland or humus soils. Method: Ginsenoside was extracted to determine the total content of ginsenosides with the ultraviolet spectrophotometry, and 8 kinds of monoginsenosides were measured with HPLC. Result: The content of total ginsenosides from two kinds of soils, were more than 6% and the content of monoginsenosides in humus soil was more than that in farmland soil. Conclusion: The total ginsenosides content is not different obviously; but the monoginsenoside Rc and Re in humus soil is higher.关键词：different soil;Panax quinquefolium;total ginsenosides;monoginsenosides
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical components of the essential oil extracted from two cultivars of Chrysanthemum morifolium (C. morifolium Ramat "Hongxinju" cv. nov. and C. morifolium Ramat "Huangju" cv nov.) cultivated in Jangsu province and provide scientific basis for their quality control. Method: The essential oil of the two cultivars of C. morifolium were extracted by water steam distillation and separated by GC capillary column chromatography. The components were quantitatively determined with normalization method, and identified by GC-MS. Result: From the C. morifolium Ramat "Hongxinju" cv. nov. and C. morifolium Ramat "Huangju" cv nov., 217 and 167chromatographic peaks were detected, among them 73 and 64 components were identified, which were composed of 67.36% and 62.75% of the total essential oil,respectively. Conclusion: 2-carene, cis-ocimene, gamma.-terpinene, camphor, borneoll, trans (. beta.)-caryophyllene, zingiberene, farnesene, caryophyllene oxide and junipercamphor were identified in the essential oil of the two cultivars C. morifolium Ramat "Hongxinju" cv. nov. and C. morifolium Ramat "Huangju" cv nov.. Among them, the amounts of junipercamphor were the most which were composed of 12.64% and 10.87% of the total essential oil, respectively. Trans (. beta.)-caryophyllene is the second, which were composed of 6.45%and 4.38% of the total essential oil,respectively. There are marked differences in the compounds between the two samples. The results provide scientific basis for quality control of Chrysanthemum morifolium which are cultivated in Jangsu province.关键词：Chrysanthemum morifolium cultivated in Jangsu;cultivar;essential oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To investigate the chemical constituents from the lateral roots of Aconitum carmichaelii. Method: A combination of various chromatographic techniques including column chromatography over silica gel, MCI, and Sephadex LH-20 were used to isolate the chemical constituents and their structures were elucidated by spectroscopic data analysis. Result: Twelve compounds were isolated and identified as nicotinamide (1), uracil (2), hypoxanthine (3), adenosine (4), uridine (5), 5-hydroxymethyl-pyrrole-2-carbaldehyde (6), oleracein E (7), cis-p-coumaric acid 4-O-β-D-glucoside (8), cis-feruloyl-4-β-glucoside (9), trans-feruloyl-4-β-glucoside (10), isomaltol-glucoside (11) and 2,4,6-triphenylhex-1-ene (12). Conclusion: All the compounds were obtained from the genus Aconitum for the first time except compound 2.关键词：Aconitum carmichaelii;lateral roots;chemical constituents;alkaloids;phenylpropanoid glucosides
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发布时间：2024-01-04 - 摘要：Objective: To analyze the content changes of safrole, methyleugenol and asarinin by different processing methods in Asari Radix et Rhizoma. Method: Safrole, methyleugenol and asarinin were determined by Agilent Eclipse XDB-C18(4.6 mm×150 mm, 5 μm) column as stationary phase, 285 nm as detective wavelength, MeOH-H2O as mobile phase; the flow rate was 1.0 mL·min-1; the column temperature was kept at 35℃. Result: The linear ranges of methyleugenol, safrole and asarinin were 0.103 7-2.074 μg (r=0.999 9), 0.110 1-2.202 μg (r=0.999 9), 0.054 3-1.086 μg (r=0.999 9) and the average recoveries were 98.74%, 96.93%, 101.92% and RSDs were 2.10%, 1.58%, 3.55% respectively. Conclusion: The content of safrole decreased signigicantly in stri-fied produts, while the content of methyleugentol and asarinin was conserved effectively. Therefore, it is very sensible to stir-fire Asari Radix et Rhizoma.关键词：Asari Radix et Rhizoma;processing;safrole;methyleugenol;asarinin;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To investigate the condition and parameters of adsorption and purification of total flavones contained in Viola tianshanica by macroporous resin so as to provide methods for the enrichment of flavonoids. Method: The adsorption properties of 3 kinds of macroporous resins for total flavonoids of V. tianshanica were compared; the yield and purity of total flavonoids from V. tianshanica were considered as standards, the purification process of total flavonoids in V. tianshanica by macroporous resin was selected. Result: It was found that theadsorption properties of AB-8 macroporous resins for total flavonoids of V. tianshanica were the best, up to 48 mg·g-1 wet resin, the best conditions to elute the total flavonoids adsorbed by AB-8 macroporous resin was 35% ethanol, resin vs. crude drug was 2.5: 1, ratio of diameter to height was 1:8.Using this process, the yield of the total flavones of V. tianshanica was up to 2.08%, the purity was 71.1%. Conclusion: This process is simple and practicable, it can be used to purify the total flavones contained in V. tianshanica.关键词：Viola tianshanica;flavonoids;macroporous resin;purification;Uighur medicine
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发布时间：2024-01-04 - 摘要：Objective: To establish a simple, rapid and accurate method of protein content analysis of polysaccharide from Sipunculus nudus. Method: Using Kjeldah method and Coomassie brilliant blue method to test protein content of the polysaccharide from S. nudus. Also the precision and accuracy of the two methods were tested. Result: The content of protein was 2.40% and 2.22% by the two method and the average recovery rates were 98.69% and 100.97% respectively. The content by the two methods was almost the same and had no marked difference (P>0.05). Conclusion: In summary, Kjeldah method is simple and accurate and could assay the protein content of polysaccharide from S. nudus rapidly.关键词：polysaccharide from Sipunculus nudus;Kjeldah method;Coomassie brilliant blue method
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the quality of different specifications and different parts for sale to collect. Method: HPLC method, Kromasil Eternity-5-C18 chromatographic conditions (4.6 mm×250 mm,5 μm)chromatographic column; the mobile phase consisted of acetonitrile (A)-0.4% acetic acid solution (B); gradient elution:0.01 min, 15%A, 0.01-20 min, 20-25%A, 35 min, 30%A, 35-40 min, 40%A, 50 min, 40%A; 50-70 min, 15%; volume flow rate of 1 mL·min-1, column temperature was 30℃, the detection wavelength was 280 nm. Result: The Baicalin from the processed strip type Radix scutellariae was 66% higher than the processed Radix scutellariae with xylem rotten. The Baicalin from the pieces of processed strip type Radix scutellariae was 4.06% lower than the pieces of processed Radix scutellariae with xylem rotten. The processed Radix scutellariae with xylem rotten part of almost no Baicalin. The content of baicalin,that below 4 mm Scutellaria baicalensis and fibrous root in about 12%. Conclusion: The processed strip type Radix scutellariae was more expensive than processed Radix scutellariae with xylem rotten. Suggestions of below 4 mm Scutellaria baicalensis and Fibrous root medicinal. The processed Radix scutellariae with xylem rotten part not be medicinal.关键词：traditional standard;different part;HPLC;quality evaluation
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for the determination of moisture content in Eriobotrya japonica by NIRS. Method: The moisture content of 173 samples was determined by the method of drying under oven. NIR spectrograms were measured, the first derivative was used as spectral preprocessing options, and calibration model of moisture content was established by the partial least squares regression analysis. Result: The correlation coefficients, the root-mean-square error of calibration, the root-mean-square error of prediction and the root-mean-square error of cross-validation of the calibration model for moisture content were 0.965 86, 0.082 5, 0.137 and 0.152 63. Conclusion: The method is steady, accurate, and can be used to predict moisture content of Eriobotrya japonica rapidly.关键词：Eriobotrya japonica;loquat;NIRS
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发布时间：2024-01-04 - 摘要：Objective: By reversed phase high performance liquid chromatographic method for the simultaneous determination of propolis pinocembrin, chrysin and galangin concentration. Method: YMC-C18 column (4.6 mm×250 mm, 5 μm) was used with acetonitrile-0.1% phosphoric acid (40:60) as mobile phase. The flow rate was 1.0 mL·min-1 detected at 268 nm for chrysin and galangin, 290 nm for pinocembrin. The column temperature was maintained at 30℃. Result: Pinocembrin, chrysin and galangin were linear respectively in the range of 4.266-426.6, 4.59-459, 4.322-432.62 mg·L-1. The average recovery rates (n=6) were 98.28%, 98.82% and 100.77%; RSD was 0.89%, 0.90% and 1.32%. Conclusion: The method is simple, accurate, reproducible, and can be as propolis quality control of Chinese medicinal materials and provide experimental basis.
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard for Ershiwei Roudoukou pills (Myristicae semen, Aquilariae Lignum Resinatum, Caryophylli Flos and Carthami Flos etc.). Method: Olibanum, Caryophylli Flos, Dalbergiae Odoriferae Lignum, Aquilariae Lignum Resinatum were identified by TLC and HPLC was applied to the quantitative determination of dehydrodiisoeugenol. The method was performed on a Comatex C18 column (4.6 mm×250 mm, 5 μm) with methanol-water (68:32) as the mobile phase. UV detection wavelength was set at 274 nm; the column temperature was kept at 35℃ and the flow rate was maintained at 1.0 mL·min-1. Result: The TLC sports were fairly clear, and the blank test showed no interference. Dehydrodiisoeugenol showed a good linear relationship within the range of 0.036 8-0.184 μg (r=0.999 6). The average recovery was 100.25% and RSD was 1.16%. Conclusion: The methods are simple, sensitive, reliable, reproducible, and can be used for the qualitative and quantitative control of Ershiwei Roudoukou pills.关键词：Ershiwei Roudoukou pills;Myristicae Semen;dehydrodiisoeugenol;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: The purpose of this study was to analyze the fatty acid from Mactra veneriformis. Method: The fatty acid in flesh was extracted by the method of Bligh-Dyer. Fatty acid was methyl esterified and then analyzed by gas chromatography-mass spectrometry. Result: Twenty\|two components were identified, representing 82.51% of the total constituents, in which the saturated fatty acid represent 24.35% and the unsaturated fatty acid represent 58.16%. The major components in saturated fatty acid were octadecanoic acid and hexadecanoic acid, the major components in unsaturated fatty acid were 9,12-hexadecanoic acid, 11-eicosenoic acid, tetradecenoic acid and 5,8,11,14-eicosatetraenoic acid. Conclusion: M. Veneriformis is rich in facty acid and can be developed in the future.关键词：Mactra veneriformis;fatty acid;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To analyze and compare the chemical components of volatile oil in Eucommia ulmoides leaf from Guizhou Zunyi, Sichuan Mianyang and Henan Xinyang. Method: The volatile oil was extracted from E. ulmoides leaf by supercritical CO2 fluid extraction. The components of volatile oil were separated and analyzed by GC-MS. Result: Thirty eight components in the volatile oil of E. ulmoides leaf were identified and accounted for 80.62% of the total components in Guizhou Zunyi; 59 components in the volatile oil of E. ulmoides leaf were identified and accounted for 83.77% of the total components in Sichuan Mianyang; 30 components in the volatile oil of E. ulmoides leaf were identified and accounted for 82.57% of the total components in Henan Xinyang. Conclusion: There are some differences in the component and content in volatile oil of E. ulmoides leaf from different habitats.关键词：Eucommia ulmoides Oliv. leaf;different habitats;volatile oil;supercritical CO2 fluid extraction;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To provide the foundation for reasonable utilization by analyzing the volatile constituents of Cassia occidentalis. Method: The volatile oil was extracted from C. occidentalis by team distillation. The chemical constituents were analyzed by GC-MS. Result: One hundred and one compounds were separated and eighty-six of them were identified, which accounted for 90.07% of all the volatile oil. The major components were geranylacetone (16.84%), β-ionone (11.68%), 6-methyl-5-hepten-2-one (5.37%), phytol (3.70%); 6,10,14-trimethyl-2-pentadecanone (3.67%), farnesyl acetone (3.36%), hexanal (3.12%), α-ionone (3.12%). Conclusion: The volatile oil was extracted and analyzed from C. occidentalis by GC-MS.关键词：cassia occidentalis;Steam Distillation;GC-MS;volatile oil
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发布时间：2024-01-04 - 摘要：Objective: To investigate the changes of active composition in different parts and growth stages of Scutellaria baicalensis in Shandong Province. Method: HPLC technology was employed to detect the content of active composition in two-years old plant samples which collected from Laiwu and Pingyin in five different growth stages. Result: The content of scutellarin got to the highest value of 15.46,11.83 mg·g-1 in the part of aboveground in the beginning of flowering stage and leaf-expansion stage, respectively. The content of baicalin and wogonoside in the root which collected from Laiwu decreased in the whole growth stage, the content was 173.60,34.31 mg·g-1respectively, while the content in the samples collected from Pinyin was 176.40, 212.94 mg·g-1in flower stage and 28.93, 34.21 mg·g-1 in mature stage respectively. The content of baicalein in the sample of Laiwu was 8.05,9.49 mg·g-1in the beginning of flowering stage and maturity stage respectively. The content of wogonin was 1.94,3.75 mg·g-1 in the beginning of flowering stage and withering stage respectively, while the content was 12.80, 8.27 mg·g-1and 2.70, 1.48 mg·g-1 in leaf-expansion and withering stage in the samples collected from Pingyin. Conclusion: The content of scutellarin in aboveground is higher than that in underground which opposite to the content of baicalin; the content of baicalin and wogonoside from leaf-expansion to withering stage shows a trend of M style, while the content of baicalein and wogonin shows a trend of N style.关键词：Scutellaria baicalensis;growth stage;different parts;active composition;content
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发布时间：2024-01-04 - 摘要：Objective: To establish a rapid analytical method for moisture in Xiaoyao pills(condensed) by near-infrared spectroscopy. Method: The near-infrared spectral data of 97 samples were collected by Nicolet 6700 NIR spectroradiometer, and the data of moisture content were obtained by oven-drying method. Then, the multivariate calibration model for moisture was established by patrical least square (PLS) with oven-drying method analysis values as reference, and predicting the moisture content of unknow samples. Result: The correlation coefficients (R2) of the quantitative calibration model for moisture was 0.953 52, the root-mean-square error of calibration (RMSEC) was 0.132, the root-mean-square error of prediction (RMSEP) was 0.177. Conclusion: The method is simple, non-polluted, accurate, which could be applied for the fast determination of moisture in Xiaoyao pills(condensed).关键词：near-infrared spectroscopy;partial least square;xiaoyao pills (condensed);moisture;rapid determination
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发布时间：2024-01-04 - 摘要：Objective: To analyze the components of fatty acids in the seeds of Abutilon theophrasti and develop a method for the simultaneous determination of the main fatty acids. Method: Samples were extracted by Soxhlet extraction. After methyl esterification treatment, fatty acids were identified by GC-MS and determined by GC with a FID detector and methyl heneicosanoate as internal standard. Result: Total 10 kinds of fatty acids were identified, accounted for 99.43% of the total fatty oil. The calibration curve was linear within the range of 33.6-336 mg·L-1 (r=0.999 0), 6.58-65.8 mg·L-1 (r=0.999 9), 33.4-334 mg·L-1 (r=0.999 8), 128.3-1 283 mg·L-1 (r=0.999 5) for palmitic acid, octadecanoic acid, oleic acid and linoleic acid respectively. The average recoveries (n=9) were 100.4%, 100.6%, 101.6% and 101.0% for palmitic acid, octadecanoic acid, oleic acid and linoleic acid respectively. Conclusion: Ten kinds of fatty acids are identified and a method for the determination of palmitic acid, octadecanoic acid, oleic acid and linoleic acid in Abutilon theophrasti simultaneously is developed. The method is sensitive, accurate and reproducible.关键词：Abutilon theophrasti Medic.;GC-MS;GC;fatty acid;determination
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发布时间：2024-01-04 - 摘要：Objective: The purpose was to determine the content of ellagic acid and raspberry ketone extracted from red raspberry (Rubus corchorifolius) fruit by HPLC. Method: The chromatography conditions of ellagic acid were as follows:C18 column of ODS CAPCELL PAK (4.6 mm×250 mm, 5 μm), CH3OH-0.2%H3PO4 (45:55) at a flow rate of 1.0 mL·min-1 as mobile phase and detection wavelength of 254 nm; the chromatography conditions of raspberry ketone were as follows:C18 column of ODS CAPCELL PAK (4.6 mm×250 mm, 5 μm), CH3OH-H2O (30:70) at a flow rate of 1.0 mL·min-1 as mobile phase and detection wavelength of 271 nm. Result: The results showed that there was a good linearity between the peak area and the content of ellagic acid in the range of 0.056-0.402 g·L-1 (r=0.999 4). The average recovery of sample was 100.2%, and RSD was 1.5%. The results showed that there was a good linearity between the peak area and the content of raspberry ketone in the range of 0.014 4-0.033 6 g·L-1 (r=0.999 4). The average recovery of sample was 99.4%, and RSD was 1.8%. Conclusion: The method is simple, accurate and reproducible for determination ellagic acid and raspberry ketone in the extract of red raspberry fruit.关键词：red raspberry;ellagic acid;raspberry ketone;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determination of hydroxysafflor yellow A in Mongolian medicine Huricha-6 pills. Method: Hydroxysafflor yellow A in Huricha-6 pills was determined by HPLC method using the column of Diamonsil C18(4.6 mm×200 mm, 5 μm) through which a mobile phase consisting of methanol-acetonitrile-0.7% phosphoric acid solution (26:2:72) was pumped at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 403 nm and the column temperature was 30℃. Result: The linear range of hydroxysafflor yellow A was 70-420 ng (r=0.999 8). The average recovery rate was 99.27% (RSD 0.86%). Conclusion: The method is simple, accurate and reproducible, which can be used for the quality control of hydroxysafflower yellow A.关键词：Huricha-6 pills;hydroxysafflower yellow A;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the qualitative and quantitative methods of Babao Jingfeng San. Method: Artificial muskin Babao Jingfeng San were identified with the method of GC, The contents of Eugenol and Borneol were determined with the method of GC. Using SUPELCO SUPELCOWAXTM-10 quartz capillary column, gradient temperature, inlet temperature: 175℃,detector temperature: 240℃。Result: In GC, Artificial musk can be detected and without interference. Quantitative analysis of GC showed that the linear ranges of Eugenol and Borneol were 0.020 12-0.100 6 μg (r=0.999 3) and 0.080 33-0.401 65 μg, (r=0.999 1),respectively. Conclusion: The methods set up by this study are accurate and easy to perform with the merits of good resolution, specificity and reproducibility. It can be used for the qualitative and quantitative detection of Babaojingfeng San.关键词：Babao Jingfeng San;GC;artificial musk;eugenol;borneol
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发布时间：2024-01-04 - 摘要：Objective: To isolate and identify chemical constituents from Phellodendron chinense. Method: Compounds were isolated by silica gel and Sephadex LH-20 column chromatography, and their structures were determined by means of the spectral analysis and physicochemical properties. Result: Eleven compounds were isolated and identified as berberine(1), obaculactone(2), shihulimoninA(3), N-p-coumaroyltyramine(4), 1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxy-propyl)-2-methoxyphenoxy]-propane-1,3-diol(5), phellodendrine(6), magnoflorine(7), palmatine(8), jatrorrhizine (9), columbamine (10), obacunone (11). Conclusion: Compounds 3 and 5 were isolated from P. genus for the first time, and compound 4 was isolated from this plant for the first time.关键词：Phellodendron chinense;chemical constituents;structural identification;isolated for the first time
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发布时间：2024-01-04 - 摘要：Objective: To study the chemical constituents of Callicarpa longissima. Method: Column chromatography was used to isolate and purify the constituents, whose structures were identified on the basis of spectral data analyses. Result: Ten compounds were isolated and identified as ursolic acid (1),oleanolic acid (2),seco-hinokiol(3),uvaol(4),erythrodiol(5),euscaphic acid (6),maslinic acid (7),acacetin (8),β-sitosterol (9),daucosterol (10). Conclusion: Compounds 2-10 are isolated from C. longissima for the first time.关键词：Callicarpa longissima;chemical constituents;terpenoids;seco-hinokiol
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC fingerprint for the identification of Peach Juvenile Branchlet. Method: The analysis was carried out on a Diamonsil C18(4.6 mm×250 mm,5 μｍ) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid,gradient eluent,the flow rate of 0.5 mL·min-1.The wave length was 290 nm, data were analyzed by the main component of dried branch of Prunus persica and peach leaf and peach seed P.E.to compare the similarity of samples. Result: The mutual mode to HPLC fingerprints was estabilished and the 47 mutual peaks were indicated. The similarities among the of the main component of dried branch of P.persica and peach leaf and peach seed P.E.collected from different sources were obtained. There was an apparent difference in fingerprint. Conclusion: HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Peach Juvenile Branchlet.关键词：peach juvenile branchlet;HPLC;fingerprint;identification
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发布时间：2024-01-04 - 摘要：Objective: To establish a RP-HPLC method for the determination of related substances in ezetimibe. Method: The method was performed on a Phenomenex Luna C18 column (4.6 mm×150 mm, 5 μm) using a mobile phase consisting of 0.01% phosphoric acid as the mobile phase A, acetonitrile as the mobile phase B with gradient elution. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 231 nm. The column temperature was set at 35℃. Result: Related substances were completely separated from ezetimibe. The standard curves of substances A, B and C were linear in the range of 0.035-0.8 mg·L-1(r=0.999 7, 0.999 4, 0.999 9) (n=6), respectively. Conclusion: The method is simple, sensitive with high specificity and repeatability. It is suitable for the determination of related substances in ezetimibe.关键词：ezetimibe;related substances;RP-HPLC;gradient elution
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发布时间：2024-01-04 - 摘要：Objective: To study the volatile oil in one-year-old and two-year-old of Radix Angelicae Sinensis (RAS). Method: The volatile oil is obtained by steam distillation, their chemical constituents is analyzed by GC-MS. Result: The content of volatile oil was 1.042% in one-year-old of RAS, which was much higher than the content of volatile oil 0.664% in the two-year-old of RAS. And their color was completely different. The chemical composition of volatile oil was basically consistent. In one-year-old of RAS the content of ligustilide was 54.01%,while the content of ligustilide was 46.06% in the two-year-old of the RAS. In the two-year-old of RAS, the content of 1,4-cyclohexadiene-1,2-dicarboxylic anhydride and butylidene phthalide were 11.22% and 14.19%, significantly above the 2.49% and 7.02% in the one-year-old of RAS. Terpenes of one-year-old of RAS were 27.85%, markedly higher than the 12.58% from the two-year-old of the RAS. Conclusion: The one-year-old of RAS have certain development value in the extraction of the content of volatile oil.关键词：one-year-old of Radix Angelicae Sinensis;two-year-old of Radix Angelicae Sinensis;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: Experiments were carried out to study the mature seeds of Gentiana rigescens, and the effects of phosphorus on growth and physiological characteristics of G. rigescens seedlings in the floating system were explored. Method: Perlite was mixed with vermiculite, peat, et al, to form the seedling substrate, and the effects of phosphorus on growth and physiological characteristics of G. rigescens seedlings in the floating system were studied by floating nursery law and plant physiology experimental method. Result: The suitable concentration phosphorus was used to treat the seedlings of G. rigescens in the floating system, not only make the seedling plant height, root length, stem and leaf dry weight, root dry weight significantly higher than those not treated with phosphorus (P<0.01), but also make the leaves chlorophyll content, nitrate reductase(NR) activity and root activity were significantly higher than those not treated with phosphorus (P<0.01). Conclusion: This study ascertained the effects of phosphorus on the botanical characters and physiological characteristics of G. rigescens seedlings in the floating system, and the optimal phosphorus concentration in the floating system nutrient solution of G. rigescens is within the range between 50 mg·L-1 and 100 mg·L-1.关键词：Gentiana rigescens;floating system;phosphorus;growth and development;physiological characteristics
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发布时间：2024-01-04 - 摘要：Objective: To study the tissue distribution of multiple effective components contained in Qishe pill in rats. Method: The heart, liver, lung, kidney, brain and muscle were collected 30 min, 1,2,3,4,6 h, after SD rats were given 0.675 g·kg-1 Qishe pills, then HPLC-MS/MS was used to detect 11 components' concentration in tissues. Result: Flavonoids in Qishe pill was mainly concentrated in brain (calycosin 5.33 μg·g-1, calycosin-7-glucoside 4.94 μg·g-1, formononetin 3.61 μg·g-1, ononin 1.24 μg·g-1) and lung (calycosin 1.38 μg·g-1, calycosin-7-glucoside 15.33 μg·g-1, formononetin 0.29 μg·g-1, ononin 53.75 μg·g-1), alkaloids was in muscles (sinomenine 347.06 μg·g-1, tetrandrine 706.69 μg·g-1, fangchinoline 589.07 μg·g-1), terpenes and organic acids were in brain (senkyunolide A 5.75 μg·g-1, senkyunolide I 0.59 μg·g-1, ferulic acid 2.4 0 μg·g-1, cholic acid 56.82 μg·g-1). Conclusion: A variety of active ingredients in Qishe pills has a tendency of the lesion site.关键词：Qishe pill;multiple effective components;tissue distribution
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发布时间：2024-01-04 -
Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats
摘要：Objective: To investigate distribution characteristics of active ingredients from Scutellariae Radix in heart,liver, stomach, lung, spleen,kidney,and small intestine after rats being intragastric administrated Gegen Qinlian decoction. Method: LC-MS/MS was employed to determine the contents of baicalin,baicalein,wogonoside and wogonin in rat tissue,Shiseido CAPCELL PAK C18 column(2.0 mm×100 mm,5 μm) with a linear gradient system composed of 0.1% formic acid acetonitrile-0.1% formic acid water solution,ESI under positive ion and multi-reaction monitoring mode(MRM) were adopted.Dose of Gegen Qinlian decoction was 15 g·kg-1,2,4,6,8,12 h after administration,rats were sacrificed by breaking arteria carotis,then removed heart,liver,stomach, lung,spleen,kidney and small intestine immediately,distribution of active ingredients in each tissue was determined. Result: Linear range of baicalin,baicalein,wogonoside and wogonin were 6.150-3 077,3.030-1 515,6.170-3 085,3.140-1 569 μg·L-1,RSD of the intra was 5.4%-11.7%,RSD of inter-day precision 7.8%-11.9%,RE of accuracy was-6.4%-7.5%,average recoveries of four active ingredients were more than 75.5%,matrix effects could be ignored,the concentration of four active ingredients in liver tissue were stable under different conditions. Conclusion: Active ingredients concentration was quite different in various tissues,major distribution organs of baicalin,baicalein,wogonoside,wogonin in rats were liver,stomach and small intestine,followed by kidney,heart,lung and spleen.关键词：Gegen Qinlian decoction;baicalin;baicalein;wogonoside;wogonin;LC-MS/MS;tissue distribution;matrix effects- 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate pharmacokinetics process of the major components of Guangtongxiao foam aerosols tetrahydropalmatine (THP) and berberine hydrochloride (Ber) in human. Method: Using LC-MS/MS to measure plasma concentration at different time after rectal administration concentration of Guangtongxiao foam aerosols was determined,Win Nonlin software was used to calculate pharmacokinetic parameters. Result: In the range of 0.000 594-1.188,0.000 536-1.072 μg·L-1,THP, Ber showed good linearity,high,medium and low recovery rates of THP, and Ber, day and inter-day precision met the demands of quantification, Tmax were (132.5±25.5), (132.5±30.1)min,Cmax were (0.55±0.30),(0.31±0.32) μg·L-1. Conclusion: LC-MS/MS had good selectivity,high sensitivity for the determination of plasma sample, and can be used successfully in the study of the dynamics of human plasma that contain Guangtongxiao foam aerosols.关键词：Guangtongxiao foam aerosols;tetrahydropalmatine;berberine hydrochloride;LC-MS/MS
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发布时间：2024-01-04 - 摘要：Objective: To observe the survival and differentiation of bone mesenchymal stem cells(BMSCs) into vascular endothelial cells in brain of cerebral ischemic rats after transplantation and the effect of Naomaitong(NMT) on the survival and differentiation of BMSCs. Method: Rats whole bone marrow were cultivated and BMSCs were purified and increased by methods of adherence and selection in vitro. Rats were randomly divided into different groups. Middle cerebral artery occlusion (MCAO) model was duplicated with nylon thread. Rats of transplantation and combination groups were transplanted with BMSCs via carotid artery at 24 h after operation. Electron microscopic was used to observe the microvascular endothelial cell ultrastructure of brain tissue after BMSCs transplantation. CD31+ expression in brain tissue was detected in this expertment, and the changes of Brdu/FⅧdouble labeled cells were observed by using the method of immunohistochemistry double labeling. Result: CD31+ expression was significant in brain of sham operation group, while the expression of CD31+ was significantly decreased in rats of each model group(P<0.01). In brain of 14,28 d transplantion groups, the expression of CD31+ increased obviously (P<0.01). In comparison with that of transplantation group, CD31+ expression in brain of each combination groups enhanced significantly, especially in 28 d group(P<0.01).In brain of transplantation and combination groups, survival of Brdu mark cells could be detected, and the cells increased obviously in brain of 14,28 d combination groups compared with that of transplantation group. Survival of the transplanted cells being labeled doulely by Brdu (Brdu/FⅧ) was observed in transplantation and combination groups, and the cells increased obviously in brain of each combination group than that of transplantation group. Conclusion: It is showed the tendency that the survival of BMSCs in brain tissue increase following the extension of time after transplantation, and NMT could make more BMSCs survive. The ratio of BMSCs differentiating into vascular endothelial cells enhance firstly and then weaken with the trend of the time in rats' brain after transplantation. NMT could make the servival of BMSCs enhanced after transplantation and make the differentiation into vascular endothelial cells angiogenic effect increase.关键词：cerebral ischemia;Naomaitong;BMSCs;vascular endothelial cells;differentiation
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发布时间：2024-01-04 - 摘要：Objective: To explore the impact of Rubus chingii Hu's effective parts on kidney-yang deficiency Alzheimer's disease(AD) rats caused by D-galactose combined with hydrocortisone, and clarify the active ingredient of raspberry in anti-kidney-yang deficiency AD. Method: Rats were injected intraperitoneally with D-galactose in dose of 125 mg·kg-1 for six weeks. All rats were intramuscularly injected with hydrocortisone 25 mg·kg-1 for the last two weeks to establish the rat kidney-yang deficiency AD model. The blank group and the model group were given with saline, other groups were administrated with R. chingii high-dose group(24 g·kg-1), R. chingii low-dose group(12 g·kg-1), ethyl acetate high-dose group(24 g·kg-1)and ethyl acetate low dose-group(12 g·kg-1)for 4 weeks. After Morris water maze test,acetylcholinesterase(AchE) and choline acyltransferase(ChAT) activity of the cortex was detected. The hippocampal CA1 region for HE staining was observed and tau protein content was assayed with immunohistochemical method. Result: The R. chingii high-dose group, R. chingii low-dose group, ethyl acetate high dose group and ethyl acetate low dose group could improve the learning and memory abilities reduce cortex AchE activity and increase ChAT activity. The total numbers of cells in the hippocampus CA1 area were increased, the necrotic cells and the cell necrosis rat were decreased. Pser404-tau positive cells of the hippocampus CA1 area were decreased. Conclusion: R. chingii effective parts can improve learning and memory abilities of kidney-yang deficiency AD rats by reducing AchE activity, increasing ChAT activity, protecting neurons of hippocampal CA1 area and decreasing tau protein expression.关键词：Rubus chingii;dementia;choline function;neuronprotection;tau protein phosphorylation
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发布时间：2024-01-04 - 摘要：Objective: To explore different effects in Epimedii Folium processed pieces which processed by different quality oils from Capra hircus L. or Ovis aries L. on kidney-yang deficiency syndrome mice. Method: The kidney Yang deficiency syndrome mice model was established that mice were intramuscular injected (im) large dose of hydrocortisone. Crude drug and 6 kinds of pieces processed by different quality of oils, such as oil from C. hircus or O. aries, oil in abdominal or tail, oil from rams or ewes, were used as test group. The total flavonoids of Epimedium folium was used as positive control. Negative control group and model control group were also applied in the lab. The models were created and at the same time drugs were administered. The same volume normal saline(NS) was given to negative control group and model control group by ig administration. The concentration of total flavonoid extracts was 0.46 g·kg-1 and the content of total flavonoid was 0.23 g·kg-1. The extracts were given to positive control group. Doses were given to test groups were 18 g·kg-1, which equivalent to the crude drug. Dosing volume was 15 mL·kg-1 for each group. Administration was one time a day for 9 days, successively. The signs and symptoms of kidney Yang deficiency mice, such as weight, temperature of animal 's surface, number of independent activities, were used as index. Animal holding power, clotting time, contents of superoxide dismutase(SOD) and malondialdehyde(MDA) in serum were also used. The difference of effects between different samples on kidney Yang deficiency syndrome in mice were studied. Result: It was decreasing significantly for the weight, temperature of animal's surface, number of independent activities, contents of SOD and MDA in serum in model control group in contrast with negative control group and the difference was statistically(P<0.01,in all). It was decreasing for the trend of animal holding power and clotting time. That means the model is successful. The test group can inhibit the decrease of weight, except the pieces processed by oil from C. hircus in contrast with model control group. The test group can increase the temperature and the number of activities, and all the difference were statistically(P<0.01or P<0.05). The test group can increase the content of SOD and decrease the content of MDA, especially for the pieces processed by oils from C. hircus and from O. aries, oil in abdominal and in tail. There was statistical significance (P<0.01 or P<0.05). Otherwise, it was increase for the trend of holding power, but there was no statistical significance. It had the trend to extend clotting time only in the test group of pieces processed by oil from O. aries. The weak condition of kidney Yang deficiency syndrome in mice(including Yang) have been strongly improved by the processed pieces according in contrast with the crude drug. There are no significant difference between the processed pieces. Conclusion: The effect of processed pieces group is super to crude drug. The efficacy can be affected by the different quality of oils from C. hircus or O. aries, but there is no statistical significance.关键词：Epimedii Folium processed by the oil from Capra hircus or Ovis aries;different quality of oils;kidney Yang deficiency;comparative study
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发布时间：2024-01-04 - 摘要：Objective: To investigate Baishile capsule on behavior, neuronal apoptosis in hippocampus and the expression of B cell lymphome/leukemia-2(Bcl-2) and Bcl-2 associated x protein(Bax) in chronic depression model rats. Method: Rat chronic stress depression model was established by chronic and mild unpredictable stressors. The observationt of body weight, open-field test, preference for 1% sucrose solution were carried out. SD rats were randomly divided into control group, model group, fluoxetine hydrochloride group, and Baishile capsule (2.88,1.44,0.72 g·kg-1)group,administered ig for 21 days. Neuronal apoptosis in the hippocampus of rats were assayed by TUNEL staining; the expression of Bcl-2 and Bax was observed by immunohistochemistry. Result: Compared with control group, the weight gain, horizontal and vertical activities, 1% sucrose solution in the model group decreased significantly (P<0.01), the proportion of apoptotic cells and apoptotic cell count were significantly increased in model group(P<0.01), Bcl-2 protein expression decreased (P<0.01), and Bax protein expression increased (P<0.01).Baishile capsule high and middle dose group could significantly increase the body weight at 14,21 d (P<0.01); and significantly increase the horizontal and vertical activities of score (P<0.01 or P<0.05), increase 1% sucrose solution (P<0.01 or P<0.05). Immunohistochemistry result showed that high dose group could increase the expression of Bcl-2 (P<0.05) and decrease the proportion of apoptotic cells and apoptotic cell count, and expression of Bax(P<0.05). Conclusion: Baishile capsule could play a role of antidepressant by reducing apoptosis of hippocampal neurons.关键词：Baishile capsule;depression;hippocampus;apoptosis;Bcl-2
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发布时间：2024-01-04 -
Effect and Role of Danqi Mixture on Signal Transduction of Smurf 2 in Renal Tissues of Diabetic Rats
摘要：Objective: The effective ability of Danqi mixture to inhibit diabetic nephropathy (DN) has been attested in our previous studies.However, the underlying mechanism remains largely unknown.Smad ubiquitination regulatory factor-2 (Smurf 2) is an E3 ubiqutin ligase that plays a pivotal role in regulating transforming growth factor-β1 (TGF-β1) signaling via selectively targeting Ski related novel protein N (SnoN) for degradation. In this study, we investigated the effect of Danqi mixture on expression of Smurf 2, and discuss Danqi mixture's possible mechanism in rats with DN. Method: The rat model of diabetes was induced by tail vein injection of streptozotocin.The rats were divided into three groups:the normal group, the model group and the Danqi mixture group (1.8 g·kg-1·d-1).They were orally administered once a day for 12 weeks. Result: Smurf 2 was predominantly localized to the renal tubular epithelium and interstitium of the kidney, and Western blot analysis revealed that Smurf 2 was up-regulated specifically in renal tissues from the model rats, the Danqi mixture group was significantly reduce the protein levels of Smurf 2 compared to the model group.Interestingly, Danqi mixture was also able to down-regulate the TGF-β1 and phosphorylated Smad 2 (p-Smad 2), and up-regulate Smad transcriptional corepressor SnoN, but not phosphorylated Smad 3 (p-Smad 3) in kidney. Conclusion: these results indicate that Danqi mixture can targets Smurf 2, maybe via TGF-β1/Smad 2 signaling, thereby providing the ubiquitination degradation of SnoN, so as to mitigate renal functional disorder in DN and delay its development.关键词：Danqi Mixture;diabetic nephropathy;Smurf 2;SnoN- 2
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发布时间：2024-01-04 - 摘要：Objective: To compare the antihyperlipidemic and antioxidative effect of banana peel polyphenol and simvastatin. Method: Sixty rats were randomly divided into 6 groups, control group, model group, positive drug simvastatin group (10 mg·kg-1), banana peel polyphenol low, middle and high dose group, (30,60,120 mg·kg-1), 10 rats in each group. Control group were fed with common food, the remaining 5 groups were intragastrically given high fat diet, banana peel polyphenol was administrated at the same timefor 40 days. Then the content of cholesterol(TC), triglyceride(TG), high density lipoprotein-cholesterol(HDL-C), low density lipoprotein-cholesterol(LDL-C), malondialdehyde(MDA) and the activity of the super oxide dismutase (SOD) in serum were determined. Result: Compared with the normal control group, the rats in the model group, serum TG, TC, LDL-C, MDA were significantly increased (P<0.05), but the serum HDL-C, SOD, T-AOC activity was significantly decreased (P<0.05); compared with the model group, the banana peel polyphenols of low, middle and high dose group, serum TG, TC, LDL-C, MDA were decreased in different degree (P<0.05), serum HDL-C, SOD and the activity of T-AOC increased significantly (P<0.05). Conclusion: Banana peel polyphenol has antihyperlipidemic and antioxidative effects on hyperlipidemic model rats.关键词：banana peel polyphenols;hyperlipidemia;blood lipid;antioxidation
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of serum containing Gleditsiae Spina on inducing apoptosis and B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated x protein(Bax) mRNA expression of human colorectal cancer SW-480 cells. Method: Liquid extracted from Gleditsiae Spina was prepared, the serum containing Gleditsiae Spina was prepared by feeding healthy 50 SD rats at high, medium and low dose of Gleditsiae Spina(2 000, 1 000,500 mg·L-1). normal saline and cyclophosphamide (CTX) group(50 mg·kg-1) according to the rat body weight(20 mL·kg-1). The impact of serum containing Gleditsiae Spina on SW-480 with a cell density of 1×106/mL proliferation was estimated by methyl thiazolyl tetrazolium(MTT) at 24, 48, 72 h after intervened, the apoptosis was detected by flow cytometry(FCM) and Annexinc V-FITCPI. And the mRNA expression variance of the bax and Bcl-2 was used to detect by RT-PCR after intervention with various dose ofserum containing Gleditsiae Spina for 24 h. Result: The serum containing Gleditsiae Spina had obvious inhibition effects on human colorectal cancer SW-480 cells in vitro, the A were significantly lower than that of the blank group(0.370±0.005)%,(0.624±008)%,(1.078±0.038)%(P<0.01), the apoptosis of human colorectal cancer SW-480 cells (36.918±1.390)%,(33.036±2.972)%, (23.372±1.612)% was significantly lower than that of the blank group(5.036±0.378)% (P<0.01), the expression of mRNA of Bax(0.258±0.037, 0.442±0.024, 0.652±0.072)was significantly lower than that of the blank group (0.936±0.047)(P<0.01),whereas expression of Bcl-2(1.946±0.017, 1.810±0.023, 1.724±0.028) was higher than that of the blank group(1.260±0.029) (P<0.01). Conclusion: The serum containing Gleditsiae Spina can inhibit proliferation and induce apoptosis of human colorectal cancer SW-480 cells. The decreased expression of Bcl-2 gene and the increased expression of Bax gene may be the mechanism of apoptosis of SW-480.关键词：Gleditsiae Spina;containing serum;human colorectal cancer SW-480 cells;apoptosis;Bcl-2/Bax gene
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发布时间：2024-01-04 - 摘要：Objective: To study the effect of Xingnao Yizhi capsule with different proportions on learning and memory abilities in Alzheimer's disease rats and find the optimal proportion. Method: Male Wistar rats were randomly divided into 12 groups as following:sham operation group, model group, huperzine-A(0.05 mg·kg-1) group, Xingnao Yizhi capsule group and 8 effective parts with different proportions. After 3 days of routine breeding, Morris water maze was used to train the rats for 7 days. Alzheimer's disease model was reproduced by 1.25% D-galactose intraperitoneal injection(4 mL·kg-1)for six weeks, combined with β-amyloid protein 1-40 (Aβ1-40) hippocampal injection 2 μL(5 g·L-1). One week after modeling, drugs were administered to rats by intragastric administration once every day for a month, evaluating rat learning and memory function with different proportions by Morris water maze test. Result: Compared with the model group, all groups improved the space learning ability. The mean escape latency was significantly shortened in place navigation test and the frequency of passing through the platform was obviously increased in spatial probe test, which showed an obvious difference in Morris water maze, more obviously in Xingnao Yizhi capsule(borneol:icariin:total polygala tenuifolia saponins:total Radix notoginseng saponins 45:6.8:32.6:0). Conclusion: Xingnao Yizhi capsule effective parts with different proportions have the effect of improving space learning and memory function, while the effect intensity may be different.关键词：Xingnao Yizhi capsule;Alzheimer’s disease;ethology;Morris water maze test
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发布时间：2024-01-04 - 摘要：Objective: To compare the impact of different interventions on the behavior indicators of anxiety rats within the Elevated Plus Maze test(EPM). Method: Sixty SD male rats were randomized to group A (quiet control group, 10 rats) and model group(group M0, 50 rats). Group M0 received 21d randomal and unpredictable anxiety stimuli for modeling, while at the same time group A received no intervention. Then group M0 was randomly divided into five groups,including model recovery group,Salad oil 4.0 g·kg-1·d-1ig(M1), model exercise group, Salad oil 4.0 g·kg-1·d-1 ig(M2), model Rana egg oil group, 4.0 g·kg-1·d-1ig(M3), model exercise-Rana egg oil group, 4.0 g·kg-1·d-1 ig(M4), model drug control group,Diazepam 2.0 g·kg-1·d-1 ig(M5), 10 rats in each group. With different recovery methods each group would have a 28d recovery, and at the end of the recovery, all groups received a EPM (elevated plus maze) experiment, and behavioral indicators were collected to compare the differences. Result: The open arm movement time in group M2, M3 and M4 was (185.3±10.8), (176.8±10.6), (177.6±11.1)s, which was significantly higher than that of M1 (145.4±21.6)s. The open arm movement distance (cm) of M2, M3 and M4 was (975.5±27.1),(945.7±19.7), (947.1±18.9)cm, which was significantly higher than that of M1 (732.9±18.9)cm; the open arm entries of M2, M3 and M4 were (10.9±2.4), (11.3±1.5), (11.6±1.1), which was significantly higher than that of M1 (7.7±1.5),(each P<0.05). Conclusion: After a 28 d recovery period, exercise, Rana egg oil, and exercise-Rana egg oil methods could significantly relieve anxiety behavior, the exercise group recovered best, but the natural recovery means had no significant effect after 28 days.关键词：EPM experiments;behavioral indicators;different intervention methods;anti anxiety;comparative study
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of total diterpenoids from Rabdosia japonica var. glaucocalyx (TD) preconditioning on myocardial ischemia/reperfusion injury (MI/RI). Method: Twenty-four rabbits were randomly divided into four groups,sham group (group A),I/R group(group B), Danshen dripping pills(DS) group(group C)and TD group (group D). Group A and B were administered distilled water (5 mL·kg-1·d-1) intragastrically daily for 7 days prior to the operation. Rabbits in group C, D underwent the same surgical procedure as those in group B, but received respectively oral DS (37.8 mg·kg-1·d-1) and TD (4.76 mg·kg-1·d-1), 7 days before the operation. Ultrastructure was observed by light microscopy and electron microscopy. TUNEL staining detected myocardial cell apoptosis index. Immunohistochemisty determined expression of apoptosis-related protein B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated x protein(Bax). Result: Apoptosis index of myocardial cells in group C and D is respectively 10.93% and 9.87%, compared with group B (18.40%)the difference was obvious(P<0.05). Immunohistopathological studies showed that A of Bcl-2 in group C, D was 41.03, 52.09,compared with group B (29.47) (P<0.05). Conclusion: TD may reduce MI/RI in rabbit model and have protective effects on myocardium.
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发布时间：2024-01-04 - 摘要：Objective: To discuss protective effect of polygala fallax in radiation injured mice. Method: Polygala fallax extract was given at dose of 2, 4, 8 g·kg-1·d-1 respectively, negative control group add equivalent normal saline as control group. After 5 days of administration, in addition to the negative control group. micereceived disposable irradiate 6 Gy of 60Co γ radiation uniformly,administration lasted 10 days. 24 h after end administration,red blood cell count (RBC),white blood cell count (WBC), platelet count (PLT), lymphocytes (Lymph), spleen index and thymus index, DNA damage of spleen cells and marrow cells were observed. Result: Compared with the negative control group, P. fallax decreased RBC,WBC, PLT,lymph thymus index, spleen index (P<0.01 or P<0.05),and increased spleen cells in bone marrow cells percentage of tail DNA and tail moment significantly(P<0.01 or P<0.05).But compared with the control group, P. fallax increased RBC count WBC PLT, Lymph thymus index, spleen index significantly(P<0.01 or P<0.05), and decreased spleen cells in bone marrow cells percentage of tail DNA and Tail Moment significantly(P<0.01). There is dose-response relationship. Conclusion: P. fallax extract have certain protective effect on hemopoietic system and immune organs in mice caused by radiation injury.关键词：Polygala fallax;60Co gamma rays;radiation injury;protective effect
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发布时间：2024-01-04 - 摘要：Objective: To investigate the mechanism of Rabdosia ternifolia on hepatic fibrosis in rats. Method: Rat hepatic fibrosis model was produced by back subcutaneous injection of 40% carbon tetrachloride(CCl4) peanut oil, once every three days, for eight consecutive weeks, at the same time the rats were given R. ternifolia extract at 20, 40, 80 g·kg-1 with gavage. Take blood After 8 weeks of administration, serum were collect, and the ELISA method was used for the determination of serum proeollagen type Ⅲ (PCIII), hyaluronic acid (HA), tissue inhibitor of metalloproteinase-1 (TIMP-1), matrix metal oproteinase-2 (MMP-2) and liver transforming growth factor-β1 (TGF-β1) concentration. The liver collagen fiber formation was observed by Masson staining. Result: Serum HA, PCIII, TGF-β1 and TIMP-1 concentration were significantly raised in model group rats than normal group rats (P<0.01), while MMP-2 was significantly decreased (P<0.01). R. ternifolia at high, medium dosage significantly reduced the content of HA, PCIII, TIMP-1, as well as increased the content of MMP-2 in serum (P<0.01); and the extract significantly reduced TGF-β1 level in liver (P<0.01). The R. ternifolia at low dosage significantly reduces the levels of serum HA, PCIII content (P<0.05); it also demonstrated a trend of decreasing serum TIMP-1 and liver TGF-β1 content, increasing of the content of MMP-2, but the difference with the model group is not significant. Conclusion: R. ternifolia effectively alleviated the degree of the chronic liver injury in liver fibrosis rat, the mechanism may be related to the regulation of TGF-β1 level and the controlling of the release of the TIMP-1 and MMP-2 in hepatic stellate cells.关键词：Rabdosia ternifolia;liver fibrosis;carbon tetrachloride
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发布时间：2024-01-04 - 摘要：Objective: To observe effect of Qiwei Wenyang softcapsule on pituitary-adrenal cortex system and pituitary-gonadal system function in animal kidney Yang deficiency model. Method: The high dose of hydrocortisone was used to establish the kidney Yang deficiency model, at the same time, Qiwei Wenyang soft capsule was given, and serum corticotrophin(ACTH) and cortisol level preputial gland, seminal vesicle organ index, the mice swimming time and hypoxia tolerance time were observed. Result: The high and medium dose Qiwei Wenyang softcapsule (0.96, 0.48 g·kg-1) could obviously increase the serum ACTH and cortisol levels(P<0.05)in rats,1 and preputial gland organ index in mice (P<0.05),Qiwei Wenyang softcapsule at dose of 0.68,0.34 g·kg-1 could obviously increase seminal vesicle organ index(P<0.05 or P<0.01); significantly prolong the swimming time and the normal pressure hypoxia tolerance time at dose of 1.35, 0.68, 0.34 g·kg-1 of mice(P<0.05). Conclusion: Qiwei Wenyang softcapsule has a protective effect on kidney Yang deficiency model.关键词：Qiwei Wenyang softcapsule;kidney Yang deficiency;Bushen Wenyang
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of licoflavone on anti-oxidant of liver tissues and expression of hepatocyte apoptosis regulation gene p53 in rats after exhaustive exercise. Method: Fifty healthy male Sprague-Dawley rats were randomized into three groups, 10 rats each group the quiet control group (NC), the exercise group (ME), the administration of licoflavone in low dose exercising group (MFGL), the administration of licoflavone in middle dose exercising group (MFGM) and the administration of licoflavone in high dose exercising group (MFGH). The rats were administrated by professional gavage once a day half an hour before exercising. This administration was continupusly gaved six days in one week, and lasted for six weeks. The low, middle and high doses are respectively 4, 8, 12 g·kg-1·d-1, while the rats in control group were administrated of pure physiological saline at the same dose. The rats were killed after 6-week training. The activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxi(GSH-Px) and the content of malondialdehyde(MDA) in liver Tissues of rats were tested by corresponding kit method. And the expression of hepatocyte apoptosis regulation gene p53 was tested by reverse transcription-polymerase chain reaction (RT-PCR) method. Result: Exhaustive swimming training induced the content of MDA was higher than that in quiet control group (P<0.05), and the content of MDA in each MFG groups was higher than quiet control group, and lower than exercising control group.The activity of SOD in exercising control group was lower than quiet control group (P<0.01), and it in each MFG groups was obviously higher than exercising control group (P<0.05). The activity of GSH-Px in exercising control group was lower than quiet control group (P<0.01), and it in each MFG groups were obviously higher than exercising control group, but lower than quiet control group (P<0.05 or P<0.01). The activity of CAT in exercising control group was lower than quiet control group (P<0.01), and it each MFG groups were lower than quiet control group, and higher than exercising control group (P<0.05). The expression of hepatocyte apoptosis regulation gene p53 was increased in rats after exhaustive swimming training; while the expression of p53 was decreased when the rats were administrated licoflavone before exhaustive swimming training. Conclusion: The supplement of licoflavone could have anti-free radical oxidation action, reduce lipid peroxidation and inhibit apotosis of liver cells, to protect the liver of rats from exhaustive exercise.关键词：licoflavone;exhaustive exercise;antioxidant enzyme system of liver tissues;apotosis;expression of gene
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发布时间：2024-01-04 - 摘要：Objective: To investigate effect of serum containing Danggui Buxue decoction on the apoptosis and related protein expression of angiotensin II(Ang II)-induced myocardial. Method: Danggui Buxue decoction was prepared by serum pharmacology method. The cardiomyocytes in the logarithmic growth phase were divided into 4 groups:control group, Ang II(10-7 mol·L-1)group, valsartan(1×10-6mol·L-1) group and 10% medicated serum (0.84 g·L-1)group, each group would be detected after 48 hours. Cell activity was measured by MTT method. Cardiomyocytes DNA damage was detected by Single-cell gel electrophoresis. Laser confocal scanning microscopy was used to detect the mitochondrial membrane potential. Western blotting was used to study the changes of p53 protein and Caspase-3 protein. Result: The myocardial cell activity(A)(0.159±0.024)and mitochondrial membrane potential(Δψm)(170.19±16.15)in Ang II group were significantly lower than those of blank control group(P<0.05),but myocardial cell DNA damage, p53(0.497±0.029)and Caspase-3(0.509±0.041) protein expression were obviously higher than that of blank control group (P<0.05); the myocardial cell activity(A)(0.197±0.035) and mitochondrial membrane potential(Δψm)(242.39±18.27)in Danggui Buxue decoction containing serum group were significantly higher than those of Ang II group(P<0.05),but myocardial cell DNA damage, p53(0.387±0.020)and Caspase-3(0.385±0.029)protein expression was obviously lower than that of Ang II group (P<0.05). Conclusion: Danggui Buxue decoction could inhibit the apoptosis of Ang II-induced cardiomyocyte hypertrophy.It may be related with mitochondria-dependent apoptotic pathway.关键词：Danggui Buxue decoction;angiotesin II;cardiomyocyte;apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To investigate anti-fatigue effect of Fufang Ejiao Jiang on mice. Mehtod: Sixty selected male mice were divided randomly into 5 groups:the control group,ginseng extract group(10 g·kg-1) and the low,medium,high dose Fufang Ejiao Jiang intervention groups(7.5,15,30 g·kg-1). Using the method of the weight loading swimming test,drugs were administrated to mice for 30 days.The contents of lactic acid(LA),hepatic glycogen,haemoglobin(Hb),malondialdehyde(MDA),lactate dhydro-genase(LDH),creatine kinase(CK),blood urea nitrogen(BUN) and the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD) in rats were dectected. Result: Compared with the control group, the low,medium and high dose groups after exercise,the content of LA,MDA was obviously reduced,the content of Hb was obviously increased(P<0.05); the medium,high dose groups had a higher content of hepatic glycogen,BUN,a lower content of CK and a higher activity of GSH-Px(P<0.05);the medium dose group had a lower content of LDH(P<0.01) and a higher activity of SOD(P<0.05). Conclusion: Fufang Ejiao Jiang can be effective in delaying fatigue and improve tissue on fatigue resistance.关键词：Fufang Ejiao Jiang;anti-fatigue;the weight loading swimming
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发布时间：2024-01-04 - 摘要：Objective: To study effect of platelet rich plasma combined with bone marrow mesenchymal stem cells at different consistency on rabbit cartilage defect. Method: Sixty rabbits were randomly divided into control group, platelet-rich plasma(PRP) group(3%), bone mesenehymal stem cells(BMSCs) group(1×106/mL)and combination group(PRP+BMSCs 1:5, 2:5, 3:5).The models of rabbit cartilage defect were made by operation.Then the models were injected combination at different consistency once a week from 2th week to 5th week after operation. The rabbit cartilage defects were observed by Masson trichrome coloration. Semi-quantitative scoring of articular cartilage were computed by Pineada. Repair of articular cartilage defect in rabbits were observed. Result: Compared with the control group, semi-quantitative scoring of articular cartilage in each combination group decreased(P<0.05). In combination groups, semi-quantitative scoring of articular cartilage was lower when PRP at higher consistency. PRP combined with BMSCs(3:5) was lowest in combination groups(P<0.05). Conclusion: PRP combined with BMSCs can effectively induce bone regeneration and improve bone tissue recovery. The recovery of articular cartilage defects was related with PRP concentration.关键词：PRP;BMSCs;rabbit cartilage defect;different consistency
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Fructus cnidii on the content of testosterone, substance metabolism and anti-fatigue ability of rats. Method: By using the model of high-intensity endurance training, 80 male Wistar rats were divided into 5 groups, 15 in each group (the rats which did not meet the requirement were removed):control group (C group), motion control group (T group), exercise+ig low-dose F. cnidii group (TML group), exercise+ig middle-dose F. cnidii group (TMM group), and exercise+ig high-dose F. cnidii group (TMH group). Gavage was performed using professional device once a day. The rats in F. cnidii groups were treated with 0.75,1.5, 4.5 g·kg-1. The rats in C and T groups were given saline of same volume. After 42 days of exhaustive swimming training, body weight, swimming time and serum testosterone and other biochemical markers were measured. Result: The body weight of the rats in F. cnidii groups increased compared with T group (P<0.05), and the swimming time was longer than T group (P<0.01), serum corticosterone was lower than the T group (P<0.05), changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups. Liver glycogen (P<0.05), muscle glycogen(P<0.01) and hemoglobin(P<0.05) in F. cnidii group were higher than in T group, but the serum urea nitrogen was lower than the T group (P<0.05). Conclusion: F. cnidii can alleviate the impact of high-intensity exercise on serum testosterone, and maintain it at normal physiological levels; it can also promote protein synthesis, inhibit degradation of amino acid and protein, and increase hemoglobin and glycogen reserves in rats exercise training.关键词：fructus cnidii;testosterone;anti-fatigue
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发布时间：2024-01-04 - 摘要：Objective: To select Mussaenda extract on animal models of inflammatory, antibacterial research and analysis. Method: The mice were randomly divided into control group, positive control group and M.parviflora extract high, medium and low dose group (24,12,6 g·kg-1) administered orally, using xylene-induced mouse ear profile swelling, granuloma; carrageenan-induced rat paw edema inflammation model, and antibacterial experiments in vitro evaluation M. parviflora extract anti-inflammatory, effect. Result: M. parviflora extract significantly inhibited mouse ear edema(P<0.05), as well as rat granuloma rat paw edema formation(P<0.05); M. parviflora extract on a variety of pathogenic bacteria were inhibited(P<0.05), the minimum inhibitory concentration (MIC50) Staphylococcus aureus 0.95 μg·mL-1, E. coli 0.75 μg·mL-1, pneumococcal 0.65 μg·mL-1, Streptococcus 0.50 μg·mL-1, Shigella 0.25 μg·mL-1. Conclusion: M.parviflora extract on acute and chronic inflammation and in vitro inhibition significantly inhibitory effect.关键词：Mussaenda parviflora extract;anti-inflammatory;anti-bacterial;animal models;edema of mouse ear;paw swelling;granuloma
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发布时间：2024-01-04 - 摘要：Objective: To explore the pharmacologic activity of Qisun-Demige-Four capsules (QD-4) by animal experiments. Method: The hyperlipidemia and atherosclerosis model of New Zealand rabbits and the obesity model of SD rats were induced by feeding with high-cholesterol diets or high-nutrition diets, the experimental animals were divided into three drug groups which were fed with QD-4(the doses of rabbits were 0.75,0.37,0.19 g·kg-1, and the doses of rats were 3.24,1.62,0.81 g·kg-1), model group, positive drug group (Simvastatin or Linoleic acid 0.5 mg·kg-1 or 0.39 mg·kg-1) and normal group. The concentrations of total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in the serums and obesity indicators were detected, while aortic atherosclerotic plaque were observed and analyzed to evaluate the effect of QD-4. Result: Compared with the nutritional obese model rats, QD-4 significantly decreased TC(P<0.01), obviously increased HDL-C(P<0.05), reduced body weight(P<0.01), shortened body length, increased tail length of SD rats(P<0.01), and inhibited the growth of fat cells (the number of cells in unit horizon was increased, P<0.01), at the same time, the strength of role were better than linoleic acid. However, the food intake of each group of animals had no significantly difference(P<0.01), TC and HDL-C in QD-4 groups were less than those in linoleic acid group. Compared with the hyperlipidemia and atherosclerosis model of rabbits, QD-4 significantly decreased TC, LDL-C(P<0.01,P<0.05), reduce the lesions of atherosclerotic rabbit, in accordance with the effects of Simvastatin. Other indexes showed no statistical significance. The experimental results showed that there were obvious dose-effect relationship and the low-dose QD-4 group didn't show significant effect. Conclusion: Mongolian medicine QD-4 can adjust the disorder of lipid metabolism, reduce body weight and inhibit scleratheroma. Results correspond to Mongolian theory about clean and decreasing dirty, reduce 'Badagan'.
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发布时间：2024-01-04 - 摘要：Objective: To study the impact of vinegar processing on toxic and pharmacological actions of Euphorbia pekinensis. Method: The different concentration samples of different polarity parts of Euphorbia pekinensis and vinegar-proccessing E. pekinensis were administered in mice. The toxic reactions were recorded. The different polar samples of E. pekinensis and vinegar-proccessing E. pekinensis were administered in mice which were divided randomly into groups, the dose of samples were 3.9 g·kg-1 (equal to crude herb). Charcoal powder movement in small intestine peristalsis, diuretic effect and anti-inflammatory effect were employed to evaluate the effects of the water and ethanol extractions and different polar parts of E. pekinensis and Vinegar-processed E. pekinensis. Result: The medium lethal dose (LD50) was calculated by SPSS 13.0.The LD50of E. Pekinensis and vinegar-processed E. Pekinensis was 160.3, 234.8 g·kg-1, its 95% confidence interval was 142.5-180.3 g·kg-1and 209.7-262.8 g·kg-1 respectively. As compared with the control group, the purgative effect and diuretic effect of the ethanol extractions and different polar parts of E. pekinensis and vinegar-processed E. pekinensis had significant differences(P<0.05). The effects of the parts of ethyl acetate is strongest(P<0.01). The purgative effect and diuretic effect of Vinegar-processed E. pekinensis werereduced. anti-inflammatory effects of ethyl acetate of vinegar-processed E. pekinensis wassignificantly increased (P<0.05). Conclusion: The ethyl acetate extraction shows toxicity of E. pekinensis and pharmacodynamics effects. Vinegar-processed E. pekinensis can significantly reduce efficacy and toxicity, increase anti-inflammatory effect.关键词：E. pekinensis;vinegar-processed E. pekinensis;acute toxicity;purgative effect;diuretic effect;anti-inflammatory effect
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发布时间：2024-01-04 - 摘要：Objective: To study the mechanism of Jiawei Wendan decoction fighting the nutritional obesity of rats by the contents of serum levels of total superoxides, such as dismutase (T-SOD), nitric oxide(NO), malondialdehyde(MDA), and the aspect of the pathological changes of liver and adipose tissue. Method: Adopting the double feeding method of high fat emulsion and general feed to copy the model of rats with nutritional obesity. Thirty male SD rats of ablactation were equally divided into the blank control group, model control group and the Jiawei Wendan decoction group. Animals in each group were fed with normal diet every day. Except the control group, animals in the other two groups were treated with intragastric administration of self-made high fat emulsion(20 mL·kg-1) every morning for 4 weeks. From the fifth week to the eighth week, Jiawei Wendan decoction 7.74 g·kg-1 was given every afternoon orally, and animals in the control group and model control group were given with the same volume of water. After the last administration, there was a fasting of 12 h, but water was available. The serum level of T-SOD, NO, MDA were tested; separating liver and adipose tissue, hematoxylin and eosin (HE) staining, and the structure and pathological change of liver, adipose cells were observed under optical microscope. Result: The serum NO content in the experimental scattered Jiawei Wendan decoction group was significantly lower than those in the model group (P<0.01); the rats' liver cell boundaries are clear and neat;with scattered fatty degeneration of liver cells; the number of fat cells increased and the volume of these cells decreased. Conclusion: The mechanism of Jiawei Wendan decoction for anti-nutritional obesity in rat may be related to the decrease in the level of serum NO, fighting against the fatty degeneration of liver cells, and reducing the volume of fat cell.关键词：Jiawei Wendan decoction;nutritional obesity;nitric oxide;mechanism study
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发布时间：2024-01-04 - 摘要：Objective: In order to study the antibacterial activity of the Broussonetia papyrifera leaf extracts. Method: The active components of B. papyrifera leaf were extracted by organic solvent, and silica gel column chromatography. The antibacterial effect in vitro of the extracts above onseveral pathogenic bacteria stains such as Staphylococcus aureus,Pasteurellamultocida, Escherichia coli, Salmonolla sp and Riemerella anatipestifer were observed, and the minimum inhibitory concentration(MIC), minimal bactericidal concentration(MBC) were also determined. Result: The result indicated that water, 70% ethanol and 50% acetone extracts of B. papyrifera leaf extracts showed better antibacterial activity, and the MIC of the water extract agaist S. aureus,Pasteurellamultocida, E. coli and Salmonolla sp were 6.75,25,6.75,12.5 g·L-1 respectively. The bacteriostatic constituents mainly exist in ethyl acetate and n-butanol extract as the B. papyrifera leaf water extract were extracted by organic solvent step by step. Moreover, 3 components had antibacterial activity agaist the pathogenic bacteria were isolated from ethyl acetate and n-butanol extract respectively. Conclusion: B. papyrifera leaf contains a variety of antibacterial ingredients, which vary in physicochemical properties, antibacterial activity and mechanism; further study on separation, identification and chemical structure determination of the compounds is expected to provide the basis for development of new antimicrobial drugs.关键词：Broussonetial papyrifera leaf;extract;antibacteria activity;MIC
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发布时间：2024-01-04 - 摘要：Objective: To observe the protective effect of Jidesheng Sheyao, a Chinese patent medicine, on liver fibrosis of mouse caused by carbon tetrachloride(CCl4) and to explore the action mechanism of the drug. Method: Forty-eight ICR mice were randomly divided into four groups:the normal control group, the model group, treatment group 1 and treatment group 2.All mice were subcutaneously injected with 40%CCl4 for 5 weeks in order to prepare liver fibrosis model. Meanwhile, the mice of two treatment groups were given with Jidesheng Sheyao in different doses and the normal the mice of control group were given with distilled water in the same volume, by intragastric administration. The concentrations of hyaluronic acid (HA) and laminin (LN) in serum of mouse were measured with the method of radioimmunoassay. Masson trichrome staining was adopted to prepare hepatic tissue section. The pathological variations in hepatic tissue were observed under light microscope and the score of hepatic fibrosis was evaluated. The rabbits were given with Jidesheng Sheyao by intragastric administration to prepare medicated serum. The hepatic stellate cell strain (HSC-T6) was taken as the model for study in vitro. HSC cells were cultured with medicated serum in different concentrations. The proliferation of HSC was assessed with CCK-8 assay. Labeled with annexin V/PI, the apoptosis of HSC was analyzed with flow cytometry. Result: Compare with mice of model group, the severities of fibrosis in mice in treatment groups were obviously alleviative,the levels of fibrosis score and the serum content of HA, LN of mice in treatment groups were all lower. After cultured with the medicated serum respectively in the concentration of 10% and 12%, the proliferations of HSC were obviously inhibited and the apoptosis rates of the cells were significantly increased, compared with the cells of control group cultured with normal rabbit serum. Conclusion: Jidesheng Sheyao can inhibit the proliferation of HSC and promote the apoptosis of HSC, so the drug has some efficacy of anti-hepatic fibrosis.关键词：Jidesheng Sheyao;hepatic fibrosis;carbon tetrachloride;hepatic setllate cell
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发布时间：2024-01-04 - 摘要：Objective: To study the antioxidant activities of several extracts of Cnidium monnieri. Method: The methanol, ethyl acetate, methylene chloride and n-hexane extracts were obtained by Soxhlet extraction techniques.The antioxidant activities were studied using 1,1-dipheny-2-picryhydrazyl free radical(DPPH) assay, nitrogen base-double- (3-ethyl benzene and dihydrogen thiazole moiety-6-sulf onic acid)ammonium salt(ABTS) assay and reducing power assay,and the total phenolic content and the total flavonoid were measured. Result: Different extracts of C. monnieri had better antioxidant activities,and the most outstanding one was found to be the methanol extract. The antioxidant activity of C. monnieri was concerned with the quantity of the total phenolic and the total flavonoid of extracts. Conclusion: The fruit of C. monnieri can be employed as natural antioxidants.关键词：Cnidium monnieri;solvent extracts;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To investigate the protective effects of Tangshenqi on diabetic nephropathy(DN) in rats. Method: The DN model was built by intravenous injection of streptozotocin(STZ) once in sprague dawley(SD)rats.The model rats were randomly divided into six groups:model control group,high-dosage Tangshenqi group(crude drug,28.0 g·kg-1), middle-dosage Tangshenqi group(crude drug,14.0 g·kg-1), low-dosage Tangshenqi group(crude drug,7.0 g·kg-1), Tangmaikang granule group, and gliquidone+captopril group. The rats were continuously administrated for 12 weeks, in the same time, the general situations in rats were observed, and body weight, food intake, water intake, urine index(urine sugar and urine protein), blood index(fasting glucose, creatinine, urea, glycosylated hemoglobin)were regularly tested. After the last administration, kidney weight, kidney index, and area of glomerular positive region by PAS staining were measured, while the examination of kidney histopathology was carried out. Result: Tangshenqi could reduce blood glucose in DN rats,regulate abnormal glucose metabolism, and promote the transformation of hepatic glycogen.It could also reduce uriinary volume, as well as the content of urine sugar and urine protein, and increase glomerular filtration rate. Moreover it could inhibit kidney hypertrophy, reduce incidence of kidney disease,as well as improve and repair the degree of lesions. Conclusion: Tangshenqi could have a good protective effect on diabetic nephropathy (DN) in rats.关键词：Tangshenqi;diabetic nephropathy;Urine protein;glomeruli;histopathology
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发布时间：2024-01-04 - 摘要：Objective: To study the effect,of big leaf Gentianae Macrophyllae Radix alcohol extract on serum anti-cyclic citrullinafer peptide(CCP)and tumor necrosis factor-α(TNF-α) in collagen induced arthritis rat control. Method: Ninty-six rats were randomLy divided into:normal group, model group, Paeoniae Alba Radix and Gentianae Macrophyllae Radix group group. In addition to the normal group, each group was given collagen emulsion multipoint to establish arthritis model. After modelling, corresponding drugs was given on day 14, 21 and 28,the general status,and joint swelling degree were observed. ELISA method was used to detect serum anti-CCP antibody and TNF-α level. Result: In model rats, ankle and digits joint swelling was obvious and serum anti-CCP antibody and TNF-α level obviously increased, in Gentianae Macrophyllae Radix group and radix paeoniae alba group,compared with model group ankle joint swelling and toes significantly reduced and serum anti-CCP antibody and TNF-α level decreased obviously, there was a statistical significance (P<0.05);after 28 days in Gentianae Macrophyllae Radix group compared with Paeoniae Alba Radix group, joint swelling of rats significantly reduced and TNF-α reduced obviously (P<0.05). Conclusion: The big leaf Gentianae Macrophyllae Radix alcohol extract can decrease in collagen induced arthritis rats serum anti-CCP antibody and TNF-α level, reduce joint swelling degree, and improve the synovial inflammation shape.关键词：big leaf Gentianae Macrophyllae Radix alcohol extract;collagen induced arthritis rats;anti-cyclic citrullinater peptide antibody;tumor necrosis factor-α
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发布时间：2024-01-04 - 摘要：Objective: To compare the uterus protein expression differences between endometriosis(Ems) correlation infertile patients and normal women of reproductive age, and study the mechanism of Bushen Huoxue recipe(BSHXR). Method: Four cases of Ems infertility patients with kidney deficiency and blood stasis syndrome diagnosed by laparoscopic surgery wereobserved. Ectopic endometria were collected during the laparoscopic surgery as Ems group. BSHXR was given to Ems patients after the surgery, lasting for three months, the ectopic endometria were collected after the treatment course as BSHXR group. Besides, four healthy women's endometria were collected three months after drug-induced abortion as normal control group. All the endometrial samples were pathologically diagnosed as secretory phase endometrium. Differential centrifugation and density gradient centrifugation method were applied to extract purified endometrial proteins. Difference gel electrophoresis(DIGE) technology were used to get the expression patterns of ectopic endometria differential proteome. Decyder difference 2-D analysis software were used to analysis. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/TOF) were used to identify among these differential protein spots. Result: Through establishing the differential proteome technology platform of ectopic endometrial proteins among 3 groups. 35 different express proteins were found by differential software analysis, 28 different proteins were identified by using mass spectrometry identification. Conclusion: Five possible mechanisms of Ems-associated infertility were predicted involving 10 different express proteins. BSHXR can regulate 26 different express proteins by 4 possible mechanisms to treat ectopic endometria of patients with Ems-associated infertility.关键词：Bushen Huoxue recipe;endometriosis;infertility;ectopic endometria;proteomics
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发布时间：2024-01-04 - 摘要：Objective: To investigate the clinical efficacy of Mahuang Fuzi Xixin tang on nephritic syndrome. Method: A total of 80 cases with nephritic syndrome were randomly assigned into control group and treatment group. Cases in control group were treated with predisone. On the basis of the therapeutic scheme of the control group, cases in the treatment group received Mahuang Fuzi Xixin tang. Result: The treatment group obviouslywas improved compared with the control group(P<0.05). Conclusion: Mahuang Fuzi Xixin tang with predisone acetate can achieve good therapeutic efficacy when treating nephritic ayndrome, the effect is better than single use western medicine predisone acetate.关键词：Mahuang Fuzi Xixin Tang;nephritic syndrome;syndrome of fighting of wind with water
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发布时间：2024-01-04 - 摘要：Objective: To investigate the therapeutic effect of Feiwei granules combined with large doses of N-acetyl cysteine on plmonary fibrosis. Method: One hundred and thirty patients with plmonary fibrosis were chose during the period from February 2010 to February 2012 in our hospital and randomly divided into two groups including control group (65 patients) with conventional hormone therapy and observation group (65 patients) with Feiwei granules combined with large doses of N-acetyl cysteine; and the clinical total effective rate, positive rate of change for chest high-resolution CT (HRCT), before and after treatment symptom scores of traditional Chinese medicine(TCM) and lung function index of both groups were compared. Result: The clinical total effective rate of observation group was significantly higher than control group(P<0.05). The positive rate of change for chest high-resolution CT (HRCT) including grid shadow and patches shadow of observation group were significantly lower than control group(P<0.05). The symptom scores of TCM after treatment in both groups were significantly better than before treatment, and the symptom scores of TCM after treatment of observation group were significantly better than control group(P<0.05). The lung function index including total lung capacity(TLC) and forced vital capacity(FVC) of observation group were significantly better than control group(P<0.05). Conclusion: Compared with conventional hormone therapy, Feiwei Granules combined with large doses of N-acetyl cysteine can efficiently improve clinical symptoms and lung function, and is worthy of clinical application value.关键词：Feiwei granules;N-acetyl cysteine;plmonary fibrosis
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of composite salvia injection combined with ulnastatin in treatments of acute pancreatitis. Method: One hundred and sixteen cases with acute pancreatitis were randomly divided into control group and experience group. Control group was given routine treatment and ulinastatin quiescent point. Experience group received composite salvia injection combined with ulinastatin therapy. Clinical efficacy, laboratory indexes and inflammatory factors were compared between two groups. Result: The effective rates of treatment in experience group were much higher than that in control group(93.3% vs 80.4%,P<0.05). Hospital stay in experience group was much less than that in control group (P<0.05); comparison with control group, blood amylase, leukocyte, interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) after treatment in experience group greatly decreased (P<0.05). Conclusion: Composite salvia injection combined with ulinastatin for acute pancreatitis greatly decrease the serum inflammatory factors and improve the clinical efficacy.关键词：acute pancreatitis;composite salvia injection;ulinastatin
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发布时间：2024-01-04 - 摘要：Objective: To observe efficacy of Pingchuang paste in treatment of chronic obstructive pulmonary disease(COPD), and explore its possible mechanism. Method: One hundred and sixty cases of COPD patients met the inclusion criteria were divided into the treatment group and the control group, 80 cases in each group. The treatment group was treated with COPD conventional therapy (bronchodilator+expectorant+Seretide 50/250 1 suction bid+antibiotics)+and Pingchuang paste; the control group received COPD conventional therapy with no Pingchuang paste(bronchodilator+expectorant+Seretide 50/250 1 suction bid+antibiotics), respectively, before and after treatment of 6 months the pulmonary function was measured,major indexes including FEV1 predicted, FEV1/FVC, 6-minute walk test and quality of life scores (application SGRQ). Result: The treatment group compared with before treatment FEV1 was improved, the difference was statistically significant (P<0.05), while in the treatment group and the control group the improvement of FEV1/FVC was not found. 6 min walk in the treatment group improved significantly compared to the control group, the difference was statistically significant (P<0.05).After treatment, the SGRQ scale declined than before treatment in two groups (P<0.05). Some symptoms, the active part of the score was declined significantly (P<0.05). Conclusion: Pingchuang paste can improve pulmonary function and clinical symptoms, and improve exercise tolerance, so as to achieve the purpose of improving the quality of life.关键词：chronic obstructive pulmonary disease;Pingchuang paste;acupiont sticking therapy;quality of life
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutical effect of Jinchan Zhiyang capsules in treating eczema of damp-heat type. Method: One hundred and ninty-three patients were randomly divided into two groups, the 98 patients in therapy group, the 93 patients in control group. The control group was treated with the maleate chlorpheniramine tablets 4 mg,Bid,combing with Using 0.1% Mometasone Furoate Cream and 0.1% Ethacridine Lactate Solution. Based on the treatment of the control group. the therapy group plus Jinchan Zhiyang capsules. The improvement of eczema skin lesions were observed with eczema area and severity index(EASI) score before and after three weeks of the treatment. Result: The clinical basically cure rate and the effective rate were 30.6%and 91.8% in the therapy group respectively, which were statistically significant higher than that of the control group (15.8% and 57.9% respectively). Conclusion: Treating eczema of damp-heat type with Jinchan Zhiyang capsules had satisfactory efficacy and was safe in clinical work. And worth to be recommended in clinical application.关键词：Jinchan Zhiyang capsules;eczema;damp-heat type;Chinese medicine therapy
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发布时间：2024-01-04 - 摘要：Objective: This study is conducted to investigate the clinical efficacy of modified Jiawei Guizhi Shaoyao Zhimu decoction on gouty arthritis. Method: There were 60 patients with gouty arthritis and two groups of treatment and control were set up. Patients in the treatment group were treated with modified Jiawei Guizhi Shaoyao Zhimu decoction and patients in the control group were treated with celecoxib. The symptoms and biomarkers for gouty arthritis were examined after two weeks treatment. Result: The total effective rate is 93.3% and 90.0% in treatment and control group respectively. There was no statistical significance, though the symptoms of gouty arthritis in treatment group were improved much better than control group. The data obtained by experimental analysis from pre-and post-therapy were analyzed. There was significant difference for inter-group comparison (P<0.05), pain,swell,limitation of acvity,treatment group were much better than control group (P<0.05).and the reduction of blood uric acid in treatment group was dramatically significant as compared with control (P<0.01). However, there was no any significant difference for the comparison of ESR and CRP between two groups. Conclusion: The modified Jiawei Guizhi Shaoyao Zhimu decoction has a significant clinical efficacy with the minimal side-effect.关键词：Jiawei Guizhi Shaoyao Zhimu decoction;gouty arthritis;curative effect observation
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发布时间：2024-01-04 - 摘要：Objective: To discuss the clinical efficacy of Pingchong Huayu Tongluo method combined with moxibustion therapy on treating endometriosis dysmenorrhea and the influence on nerve growth tactor(NGF) and prostaglandin(PGs). Method: Sixty-three patients with endometriosis dysmenorrhea were randomly divided into observation group (n=32) and control group (n=31). Patients in the two groups were both treated with moxibustion therapy. Pingchong Huayu Tongluo method was used to treat the observation group. Three menstrual cycles as one cycle. Dysmenorrhea Visual Analogue Scale(VAS) score was observed and modified B & B multidimensional scaling was used to evaluate the clinical symptoms. Serum NGF,PGF2a, PGE2 and 6-keto-PGF1a levels were tested. Result: The integrated effect of observation group(100%) was better than the control group(80.6%)(P<0.05); on the 2-3 weeks after treatment and follow-up period of the menstrual cycle, the observation group dysmenorrhea VAS scores were lower than the control group (P<0.01); After treatment, B & B multidimensional scale scores of observation group was lower than the control group (P<0.01), serum NGF, PGF2a, PGE2 levels of observation group was lower than the control group (P<0.01), 6-keto-PGF1a of the observation group was higher than the control group (P<0.01). Conclusion: Pingchong Huayu Tongluo method combined with moxibustion therapy on treating endometriosis dysmenorrhea has significant effect, its mechanism may be related to decreased NGF levels and adjust the level of PGs.关键词：endometriosis;dysmenorrhea;moxibustion;B & B multidimensional scaling;nerve growth factor;prostaglandin
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical curative effect of Tongfu Huazhuo dcoction in the treatment of early rehabilitation of ischemic stroke. Method: Ninety patients were randomly divided into control group and observation group according to the Before and after hospitalization sequence,each 45 cases. The two group were treated with western medicine on life support, medical treatment, health education routine treatment, and use traditional Chinese medicine comprehensive rehabilitation treatment, including massage, acupuncture, medicated bath. Observation group was added with Tongfu Huazhuo decoction,oral, 1 dosage/day, two groups were treated for 4 weeks.Assessed the nerve function defect severity by the United States National Institutes of Health Stroke Scale (NIHSS); assessed the limb motor function by Evaluation of Fugl-Meyer motor function scale (FMI);Assessed the activities of daily living by Daily life activities ability scale (BI); assessed the limb spasticity by the modified Ashworth spasticity scale; assessed the morbidity/mortality by the revised the Rankin scale (MRS), to measure the content of S100B protein serum. Result: Treatment of 7,14,28 days, the observation group NIHSS scores were lower than the control group (P<0.01); 14, 28 days, the observation group FMI, BI were higher than that of the control group (P<0.01),and the observation group modified Ashworth score was lower than that of the control group (P<0.01);after 3, 7 and 14 days' treatment the observation group were lower than control group (P<0.01) for S100B protein serum. After 3 months of follow-up the observation group MRS were lower than control group (P<0.01). Conclusion: In the comprehensive rehabilitation scheme, Tongfu Huazhuo dcoction improves nerve function and the motor function, enhances the capacity of life activity, reduces S100B protein serum and lowers morbidity/mortality. Tongfu Huazhuo Fang plays an important role in the early rehabilitation of stroke.关键词：ischemic stroke;early rehabilitation;integration scheme;Tongfu Huazhuo decoction;serum S100B protein
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficacy on the treatment of depression and the influence to brain-derived neurotrophic factor (BDNF) with Jieyu Anshen decoction plus Paroxetine. Method: Eighty-five patients were randomly divided into observation group (n=43) and control group (n=42). Patients in control group were treated with Paroxetine (20 mg, qd) and the dose was gradually adjusting. Jieyu Anshen decoction (one dose per day and six weeks as one cycle) was added to patients in observation group based on the treatment of the control group. HAMD, Chinese medical symptom rating, BDNF level were recorded. Result: The total efficacy of the observation group (95.34%) was significantly better than the control group (80.95%) (P<0.05). The HAMD score was decreased after treatment in two groups (P <0.01) and the score of observation group was lower than the control group (P<0.01). Serum BDNF levels in the two groups were higher after treatment in the two groups (P <0.01) and the observation group was significantly higher than the control group (P<0.01). The effective rate of Chinese medicine syndrome in the observation group (95.34%) was better than the control group (76.19%) (P<0.05). The main symptom score of the observation group were significantly lower than the control group after treatment (P<0.01). Conclusion: Treatment of Jieyu Anshen decoction combined with paroxetine in the treatment of depression could reduce the HAMD score and symptom scores, improve the clinical efficacy of disease and Chinese medical syndromes, it could also elevated serum BDNF levels, this may be an important mechanism of action.关键词：depression;Jieyu Anshen decoction;paroxetine;brain-derived neurotrophic factor
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发布时间：2024-01-04 - 摘要：Objective: To study the treatment law of lung distension, and to provide references for theoretical research and clinical prescription. Method: According to 〈Formula Dictionary of Traditional Chinese Medicine〉 and 〈Chinese Journal Full-Text Database〉, the databases of the treatment of lung distension was established to analyze the rule of clinical treatment. Result: The function wasin relieving cough,supplement of Qi,promote blood circulation to dispel blood stasis. The usage frequency of herbs was pinellia ternate(39.56%),glycyrrhiza (37.36%),almond (37.36%),ephedra (30.77%),poria(27.47%), perilla(25.27%),peach kernel (24.18%),balloonflower(20.88%),(19.78%),cassia twig(19.78%)and was based on meridian of spleen and heart. And the couplet medicines were pinellia ternate and almond, cortex mori and ephedra,pinellia ternate and poria,pinellia ternate and perilla,pinellia ternate and peach kernel,pinellia ternate and tangerine peel. Conclusion: The treatment of lung distension should invigorate the circulation of qi and the blood and to reduce phlegm.When curing the pulmonary disease, we should cure spleen and heart.关键词：lung distension;traditional Chinese medicine;treatment law;literatural study
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发布时间：2024-01-04 - 摘要：Objective: To explore medication rule for treating the liver depression and spleen deficiency of fatty liver disease based on data mining. Method: VIP, CBM and CNKI database were used to collect literatures from the foundation of th database to September 2012 and abibliography literature from 2002 to 2012 by hand on traditional Chinese medicine(TCM) treating the liver depression and spleen deficiency of fatty liver disease. The database meeting the requirement was established for data mining using frequency count, clusteranalysis and association rule to analyze regularity of key herbs and a pair (group) of herbs. Result: There were 104 prescriptions (totaling 163 herbs and 1 227 counts of frequency) in the compound herbal fomulae for liver depression and spleen deficiency of fatty liver disease. The herbs which were used for more than 4 times included 46 herbs (totaling 855 counts of frequency). They were analyzed and classified into 6 clustersincluding tonic, soothing liver drugs,strengthening spleen drugs,apophlegmatisant drugs,regulating Qi agent,absorb clots drugs and promoting digestion. The rules in pair of herbs were14, and rules in group of herbs were 62 by association analysis. The rules consisted mostly of compatibility of relieving the depressed liver and Spleen-strengthening and Damp-removing. Conclusion: Data mining is able to better explore the medication rule of the liver Depression and Spleen deficiency of fatty liver disease which better able to guide the clinic and TCM development.关键词：data mining;fatty liver disease;liver depression and spleen deficiency;medication rule
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发布时间：2024-01-04 - 摘要：Smashing tissue extraction is a new method for extracting and separating active ingredients by smashing tissue extractor,which is effective,rapid,energy-saving,easy to operate,and can be operated at room temperature.This method is mainly used for active ingredients extraction,ingredient analysis,quality standards researching and drug preparation.Its application has been involved in the pharmaceutical,food industry and agriculture and other fields.In this article,research and the applications of smashing tissue extraction in the past five years were reviewed,to provide a reference for its widespread application.关键词：smashing tissue extraction;smashing tissue extractor;extraction;separation
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发布时间：2024-01-04 - 摘要：Extraction methods of Salviae Miltiorrhizae Radix et Rhizoma from preparations in 2010 edition of Chinese Pharmacopoeia were divided into three categories,such as decoction method,ethanol refluxing method and ethanol-water extraction method,then through list classification to analyze processes,it revealed that existed improper selection of extraction solvent,excessive of extraction time,overtop of extraction temperature,extraction too many times and so on,these problems were not conducive to industrial production of preparations containing Salviae Miltiorrhizae Radix et Rhizoma,it was important to choose a suitable industrial production method for extracting Salviae Miltiorrhizae Radix et Rhizoma with efficiency and low consumption,at the same time,this article could provide a reference for process improvement of preparations containing Salviae Miltiorrhizae Radix et Rhizoma.关键词：Salviae Miltiorrhizae Radix et Rhizoma;preparations;extraction process;Tanshinone ⅡA;salvianolic acid B
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