最新刊期
卷 17 ， 期 15 ， 2011
- 摘要：Objective:The purpose of the present study was to investigate the fessibility that the essential oilas the oil phase of the microemulsion and optimize the formula of Chuan zhi microemulsion.Method:The pseudoternary phase diagrams were developed for various microemulsion formulations.The microemulsion was optimized using a three-factor,three-level Box-Behnken design.The independent variables selected were surfactant,co-surfactant and oil;dependent variables（responses） were polydispersity index（PDI）.Mathematical equations and response surface plots were used to relate the dependent and independent variables.Optimized formulation factors were selected by feasibility and grid search.Result:Validation test showed that the average particle size of antalgic microemulsion was（13.17 ± 0.07） nm.Conclusion:The essential oil can be the oil phase of the microemulsion.The Box-Behnken design can optimize the formulation objectively.关键词：Chuanxiong Rhizoma;Angelicae Dahuricae Radix;microemulsion;pseudoternary phase diagrams;Box-Behnken design
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发布时间：2024-09-23 - 摘要：Objective:To study the extraction and purification method for Ligusticum chuanxiong Hort.in Dachuanxiong Fang.Method:Refluxing extract and macroporous adsorption resin were used for extraction and purification.HPLC method was used for the determination of ferulic acid.Using pharmacodynamic test validated the effect of extract.Result:The optmial extraction conditions were as follows:alcohol concentration 70%,extraction time 2 hours,8 of solid-to-liquid ratio and extracted 3 times.HPD100 was selected as the adsorbed resin,the concentration of extract sample was 0.5 g·mL-1,6 BV 50% alcohol was chose as elution agent.The result of pharmacodynamic test indicated the extract had a good effect on sedative and analgesia.Conclusion:The method is simple and accurate,which could provide technology reference for the development of modern preparation of Dachuanxiong Fang.关键词：Dachuanxiong Fang;ferulic acid;extraction and purification;analgesic effect
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- 618
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发布时间：2024-09-23 - 摘要：Objective:To probe into the influences of different granule sizes and preparing procedures to rational clinical usage of rhubarb based on pharmacological equivalence.Method:The pharmacological equivalence of different preparing procedures,particle size（3-15 μm）,extract solvent（water and 70% alcohol）,extract time（15-20 min） and repeat times（1-6 times）,and pre-extracting or post-extracting,of rhubarb were compared regarding the defecation mass of constipated mice.Result:When extracted with water or ethanol,the maximum variations of laxative activities of rhubarb pieces in different sizes were of 4.5 or 2.0 times.Such variations of different preparing procedures,extract time,repeat times of extraction and pre-or post-extracting,were 1.7 or 1.7 times,1.3 or 2.4 times,1.9 or 1.4 times,respectively.The different preparing procedures investigated in the paper revealed significant influence to the laxative activities and those extracts were not pharmacological equivalent.When the rhubarb samples were extracted with water,the laxative activities in different repeat times of extraction revealed less difference while the post-extraction procedure revealed higher laxative activities than pre-extraction.Conclusion:The different preparing procedures for rhubarb showed significant influence to the laxative activities.The influence by clinical preparing procedures should be emphasized and the standard operation procedures（SOP） are need to be established.关键词：Rhubarb;clinical prepare procedure;standard operation procedures（SOP）;pharmacological equivalence
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发布时间：2024-09-23 - 摘要：Objective:To study the optimal methods of refining forsythiaside from leaf of Forsythia suspense Method:The ability of refining forsythiaside was compared in different types of macroporous resins and different technological conditions.Result:The AB-8 macroporous resin was the best for purifying forsythiaside in the following technological conditions:the sample concentration was 2.0 g·mL-1,the flow rate was 0.25 BV·h-1,and the eluting velocity was 3.0 BV·h-1.Conclusion:The technological condition will provide bases for the preparation technique of traditional Chinese medicine containing forsythiaside.关键词：forsythiaside;macroporous resin;purification
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- 320
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发布时间：2024-09-23 - 摘要：Objective:To optimize the formulation technology of Chonglou Kegan dropping pills.Method:On the basis of preliminary experiment,the formulation technology was selected among sort of the ratio of extractum to matrix,component of matrix,temperature of drug and drop distance,and all the procedures were evaluated by the dissolving scattered limit,weight difference,the appearance and quality of the dropping pills by means of orthogonal experimental method.Result:The optimized procedure parameters were as follows:PEG 4 000 to PEG 6 000（4:1） was used as base material with the ratio of extractum to base material at 1:4;the temperature of the drug was at 85 ℃ and the drop distance was 6cm.Conclusion:The technology is simple and feasible,and suitable for industrial production.关键词：Chonglou Kegan dropping pills;formulation technology;PEG 4000;PEG 6000;anti-influenza
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- 228
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发布时间：2024-09-23 - 摘要：Objective:To observe the bioavailability of α-asarone by its inclusion complex of hydroxypropylcyclodextrins.Method:Rabbits were divided into two groups according to different solvents.The bioavailability of α-asarone was compared between the inclusion complex of hydroxypropylcyclodextrins group and water solution group in blood drug concentration,the content of ethmoidal concha,cerebral and olfactory brain.Result:The content of α-asarone in blood,olfactory brain,ethmoidal concha and cerebral has higher solubility in the inclusion complex of hydroxypropylcyclodextrins group than that in the water solution group.Conclusion:α-asarone has higher solubility in the inclusion complex of hydroxypropylcyclodextrins group than that in the water solution group.关键词：α-asarone;bioavailability;hydroxypropylcyclodextrins
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- 356
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发布时间：2024-09-23 - 摘要：Objective:To select one or more macroporous resins that can effectively adsorb mangiferin,and then optimize the operation conditions.Method:Static adsorption test was employed to select target resins,and orthogonal test was used to optimize the operation conditions.Result:Macroporous resin D101 and AB-8 were thebest in 41 tested resins.The operation conditions,arranged from high to low,were pH,time,temperature and concentration.70% alcohol aqueous could effectively desorb mangiferin from resin D101.Conclusion:The optimal conditions for macroporous resin D101 to adsorb mangiferin are:pH 2 aqueous solution with a mangiferin concentration of 2.841 × 10-2 mol.L-1,adsorption time is 5 minutes,operating at room temperature;70% alcohol aqueous was used as desorption solution.关键词：mangiferin;macroporous resin D101;orthogonal test
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- 384
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of polysaccharides from Dendrobium nobile and to measure its effect on the splenocyte proliferation.Method:Based on the single factors test,such as extraction time,extraction temperature and solvent to material ratio were optimized by orthogonal test L9（34）.MTS/PMS assay was used to measure the splenocyte proliferation.Result:The optimal technology conditions were as follows:extraction temperature was set at 90 ℃,and extraction time was 2.5 h with ratio of 45:1.D.nobile crude polysaccharide（DNCP） and D.nobile purificatory polysaccharide（DNPP） resulted in a significant increase of lymphocyte proliferation.DNCP and DNPP could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes.Conclusion:Under these conditions the polysaccharides yield of D.nobile was up to 2.80%.DNCP and DNPP can effectively increase lymphocyte proliferation and enhance the effect of Con A,LPS-induced T and B cell growth.关键词：Dendrobium nobile;polysaccharides;extraction;orthogonal experimental design
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发布时间：2024-09-23 - 摘要：Objective:To obtain the extraction technique of mixed volatile oil from Acorus tatarinowii and Alpinia oxyphlla in Dihuang Yizhi granules and their β-CD inclusion technology.Method:The orthogonal design was applied.The yield of volatile oil was used to evaluate the extraction process by steam distillation method.The inclusion yield and volatile oil inclusion rate were used to determine the inclusion process.Result:The optimized extraction progress parameters were as follows:water volume was 10-fold,extracted time was eight hours,and soaking time was 0.5 h.The optimized inclusion progress parameters were as follows:the rate of oil:β-CD（g:mL） was 1:4,water volume was 1.5-fold,and grinding time was 60 minutes.Conclusion:The yield rate of volatile oil is greater than 1.5% and volatile oil inclusion rate is more than 95%.The optimum extraction process and inclusion process were simple and practicable,which can effectively retain the volatile oil in Dihuang Yizhi granules.关键词：Dihuang Yizhi granules;volatile oil;extraction process;inclusion process
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- 441
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发布时间：2024-09-23 - 摘要：Objective:To examine a suitable formulation for the dispersible tablets of Zanthoxylum nitidum and study the preparation process.Method:The preparative process was scanned out by single factor test and orthogonal design.A comprehensive scoring analysis was performed with disintegrating time and dissolution as the indexes.Result:The optimized dispersible tablets disintegrated in 20 seconds,and the dispersible uniform was excellent.The released rate parameters of dispersible tablets were remarkably smaller than of the control group（P<0.01）.Conclusion:Dispersible tablets of Zanthoxylum nitidum had the characteristic of fast and homogeneously dispersing and quick releasing of active constituents.关键词：Zanthoxylum nitidum;dispersible tablet;Orthogonal Design;dissolution
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- 168
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发布时间：2024-09-23 - 摘要：Objective:To optimize the best extraction of sea cucumber polysaccharide.Method:Orthogonal test was applied to study the sea cucumber polysaccharide extracted factors with its content as evaluation index.Result:The best extraction of sea cucumber polysaccharide was as follows:2 times the volume of 3% NaOH solution was extracted,and then 2% trypsin（hydrolysis conditions:pH 8.0,temperature 40 ℃,time 2 h） and 2% pepsin（hydrolysis conditions:pH 4.0,temperature 40 ℃,time 2 h） were used for dual enzymatic hydrolysis;the content of sea cucumber polysaccharide measured was 2.51%.Conclusion:The extraction method is simple,reproducible and feasible;it can be used for sea cucumber polysaccharide extraction.关键词：sea cucumber;sea cucumber polysaccharide;extraction technology
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发布时间：2024-09-23 - 摘要：Objective:To study the preparation technology of Thymus mongolicus essential oil-β-cyclodextrin（β-CD） inclusion compound.Method:It was studied with orthogonal design on the extraction of essential oil of T.mongolicus and β-cyclodextrin inclusion compound.Result:The optimum preparation technology for the inclusion compound of T.mongolicus essential oil-β-CD was using the collid mill to grind instantaneously and inclusion rapidly.The best condition was as follows:essential oils to β-CD were 1:6,the temperature for inclusion was 30 ℃,the inclusion time was 60 min.Conclusion:The method to make essential oil from T.mongolicus can easily prepare and produce β-CD on a large scale.关键词：essential oil of Thymus mongolicus;β-CD inclusion;preparation technics
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发布时间：2024-09-23 - 摘要：Objective:To optimize the molding technology of Zhuanshu Granules.Method:The effects of different excipients on the formability,hygroscopicity and solubility were investigated.Grading methods of synthesizing multiple guidelines were applied to optimizing excipients and the orthogonal experiment was used to screen out the ratio of the excipients,the concentration and usage of alcohol with formability as index.Result:The optimum composition of prescription consisted of 2 portions of extract powder,1 portion of lactose and 1 portion of dextrin;the best concentration of alcohol was 85% and the usage was 0.12 mL·g-1.The critical relative humidity was 64%.Conclusion:The molding technology is reasonable with good formability,high solubility and is not liable to moisture absorption.关键词：Zhuanshu Granules;molding technology;excipients;the orthogonal experiment
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- 138
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发布时间：2024-09-23 - 摘要：Objective:To optimize the extraction technology of Gushu capsules.Method:The optimal technology was obtained by orthogonal design test.The icatiin in decoction liquid and ethanol precipitation liquid was determined by HPLC as examination target.Result:The optimal extraction technology of Gushu capsules was as follows:decoction liquid method with 10 times volume of water,extracted 3 times and for 1 h at each time.The ethanol precipitation method with temperature is at 40 ℃,adding ethanol to 60%,laying 3 days.Conclusion:The extraction technology is rational and feasible.The method is practical and can easily control the extpaction rechnology with icariin as terget ingredient.关键词：Gushu capsule;extraction technology;icariin;Orthogonal Design
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发布时间：2024-09-23 - 摘要：Objective:To investigate moulding process of total flavonoids capsule from Lithocarpus litseifolius.Method:The moulding process of total flavonoids capsule from L.Litseifolius was selected with angle of repose,moisture absorption rate,critical relative humidity（CRH）,and bulk densityas indexes.Result:The optimum process was as follows:silica gel as excipient and its dosage was 10%,0# vacant capsule was used,CRH was 66%.These conditions could meet the requirements of capsules filled.Conclusion:The moulding process is rational and practicable.关键词：total flavonoids from Lithocarpus litseifolius;angle of repose;moisture absorption rate;critical relative humidity（CRH）;bulk density
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- 294
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发布时间：2024-09-23 - 摘要：Objective:To establish the determination of gastrodin and p-hydroxybenzyl alcohol in Gastrodia elata,and determine the different commercial grades of Gastrodia elata.Method:The samples were separated at 25℃ on ZORBAX Eclipse XDB-C18 column eluted with 0.1% phosphoric acid-methanol（95:5） as mobile phase,flowrate was 1 mL.min-1 and detection wavelength was set at 220 nm.Result:The sample quality of gastrodin and p-hydroxybenzyl alcohol with the peak area showed good linear relationship,the average recoveries were 100.78%（n=6） and 101.59%（n=6）,the content of the different origin and products specifications was significant difference.Conclusion:The method is simple,accurate and reproducibility,it can provide the basis of rational application,quality control and the standards for Gastrodia elata.关键词：Gastrodia elata;gastrodin;p-hydroxybenzyl alcohol;determination;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC-DAD method for simultaneous determination of puerarin,daidzin,liquiritin,coptisine hydrochloride,jatrorrhizine hydrochloride,baicalin,berberine hydrochloride,palmatine hydrochloride,wogonoside,baicalein,wogonin and ammonium glycyrrhizinate in Gegenqinlian decoction.Method:A Merck Chromolith RP-18e（4.6 mm × 100 mm） was used;the mobile phase was acetonitrile-0.05% phosphoric acid（contain 0.01 mol.L-1 potassium dihydrogen phosphate） in gradient elution.The detection wavelength was set at 250,275,345 nm,the flow rate was 2.0 mL.min-1 and the column temperature was maintained at 35 ℃.Result:The accuracy,precision,sensitivity,specificity and linearity of this method met the requirements.The content of the twelve active components was determined simultaneously.Conclusion:The method is rapid,simple and accurate,it has a good specificity and it can be suitable for the determination of twelve active components in Gegenqinlian decoction simultaneously.关键词：Gegenqinlian decoction;simultaneous determination;flavones;alkaloids;saponins;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To determine the content of peptide using biuret reagent and VIS-spectrophotometric method.Method:The developer is 0.02 g·mL-1 sodium potassium tartrate solution（1 mL）,5%NaOH（0.3 mL）,1%CuSO4（1 mL）.The wave length of detection is at 545 nm.Result:The calibration curves are linear at in the ranges of 2.5-10.04 mg for septa-peptide（r=0.999 7）.The average recovery is 98.02%,RSD 1.65%（n=5）.Conclusion:This method is simple.关键词：biuret reagent;visible spectrophotography;peptide;content determine
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- 400
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发布时间：2024-09-23 - 摘要：Objective:To determine the ethanol organic solvents in submicro-emulsion of the extracts from Scutellaria.Method:A simple gas chromatography（GC） method was developed with SUPELCOWAX10 polyethylene glycol capillary column with FID detector and methyl alcohol as the internal standard.The carrier gas was nitrogen.The initial column temperature was 80 ℃,then raised（8 ℃·min-1）to 120 ℃.Result:The linearity was good in the range of 0.101-1.005 g·L-1（R2=0.998 8）.The average recovery was 100.42%（RSD 2.27%）.The limit of detection was 3.03 mg·L-1.Conclusion:This GC method is proved to be accurate,sensitive and reliable,it can be used to determine the ethanol organic solvents in submicro-emulsion of the extracts from Scutellaria.关键词：submicro-emulsion of the extracts from Scutellaria;internal standard method;gas chromatography（GC）;contents of residual ethanol solvents
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发布时间：2024-09-23 - 摘要：Objective:To establish the method for determination of baicalin and geniposide in the Qingjin Huatantang.Method:High performance liquid chromatography was applied on a Agilent C18（4.6 mm × 250 mm,5μm） column at temperature 30 ℃,the mobile phase for baicalin consisted of methanol and 0.4% phosphoric acid（47:53） at detection wavelength of 280 nm,the flow rate was 0.8 mL.min-1;the mobile phase for geniposide consisted of methanol and water（25:75） at detection wavelength of 238 nm,the flow rate was 1 mL.min-1.Results:The content of baicalin in Qingjinhuatantang was 221.59 mg /dose,the content of geniposide was 335.73mg /dose.The mean recovery of baicalin and geniposide was 97.97 % and 98.36 % respectively.Conclusion:This method is simple,rapid,accurate and stable,as well as reproducible and low cost.关键词：HPLC;Qingjin Huatantang;baicalin;geniposide
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发布时间：2024-09-23 - 摘要：Objective:To establish the mutual mode of HPLC-ELSD fingerprint of butanol extracts of Fufang Fufangteng preparation,by which a new approach to assessing the quality of this medicine is provided.Method:A Shim-Pack CLC-ODS column（6.0 mm×150 mm i.d.,5 μm） with a gradient elution of acetonitrile and water was used,the flow rate was set at 1 mL·min-1.Ten batches of certified products were selected to establish the mutual mode of HPLC fingerprint of Fufangteng preparation by matching chromatographic patterns and correcting retention times.Result:This mutual mode of HPLC fingerprint can distinguish the certified Fufangteng preparation products from others by contrasting with their HPLC fingerprints.Conclusion:This method can provide more information for the quality control of Fufang Fufangteng and be used for the quality control of the saponins in this preparation products.关键词：Fufang Fufangteng preparation;HPLC-ELSD;fingerprint;quality control
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发布时间：2024-09-23 - 摘要：Objective:To establish the quality standard of Xingshen spray.Method:TLC was used too distinguish Volatile oil of Rhizoma Acori Tatarinowii,Borneolum Syntheticum and fructus Gleditsiae abnormalis.HPLC was used for the determination of β-asarone and Muscone.Result:The thin-layer chromatography spots were clear and the negative samples had no interferences,β-asarone and Muscone were linear in the range of 0.174 4-0.872 0 μg（r=0.999 8）,0.214-1.07 μg（r=0.999 4）,and the average recovery was 99.96%（RSD 2.61%,n=6）,100.17%（RSD 3.36%,n=6）.Conclusion:This method is simple,accurate,reliable and can be used as a quality control method for Xingshen spray.关键词：Xingshen spray;quality standard;TLC;HPLC;β-asarone;muscone
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发布时间：2024-09-23 - 摘要：To set up the method to control the quality of Dingxiang-Shidi Tablet.Method:TLC was used to identify ginsenoside and gallic acid.HPLC was used on a Shimazu LC-10ATVP to detect the content of eugenol;the chromatographic system consisted of octadecyl silane chemically bonded silica,methanol-water（65:40） as mobile phase.The detecting wavelength was at 280 nm.The number of theoretical plates calculated by eugenol should be no less than 3000.Result:The spots of test substance and reference substance in the chromatogram were the same.The flowing out time of peak of all the components was appropriate with good separation effect.The peak area and theoretical plates were in a high level.All of them can assure the authenticity,accuracy and repeatability of the testing results.The negative results won’t interfere the results.This method was strongly specific.Conclusion:This method can be used to control the quality of Dingxiang-Shidi Tablet.关键词：Dingxiang-Shidi Tablet;TLC;HPLC;identification;content determination
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发布时间：2024-09-23 - 摘要：Objective:To study the chemical compositions and establish chromatograph peak of effective characteristics compositions.Method:IR,MS,1H-NMR and 13C-NMR and HPLC,Different solvent extracts of Radix Tinosporae were isolated and identified.The acetic acid-induced writhing test were used to observe the analgesic effects.The anti-in flammatory effects of extracts from Radix Tinosporae were determined by using the model of xy lene-induced mouse ear edema.Aad identify the main analgesic chemical compositions HPLC peaks of Radix Tinosporae.Result:One compounds were isolated and 1 compounds were idengtfied.Established Radix Tinosporae diterpenoids spectrum and one compounds peaks of the main analgesic.Conclusion:For the first time establishing the Radix Tinosporae diterpenoids spectrum of the analgesic and anti-inflammatory activit.关键词：Radix Tinosporae;chemicai composition;analgesic effects;anti-in flammatory effects;HPLC;spectrum
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发布时间：2024-09-23 - 摘要：Objective:To provide the reference for utilization and quality standards of Magnolia Flos.Method:Fingerprints of magnolia flower bud at different varieties were studied by HPLC,the quality control standard was established for HPLC fingerprint of magnolia flower bud at different varieties.Result:The fingerprints were established for magnolia flower bud of different spring varieties with main effective ingredient magnolin as standard to provide scientific basis for development application,the quality evaluation and quality standards of different magnolia varieties.Conclusion:There is some significance for the application and quality standard of herbs magnolia flos.关键词：HPLC;Yulan magnolia flower bud;Hope spring;different varieties;magnolin
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- 363
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发布时间：2024-09-23 - 摘要：Objective:To establish the method for the assaying of loganin and puerain in Anke capsule with HPLC method.Method:The chromatographic column was Aglient Eclipse XDB-C18.The mobile phase was consisted of acetonitrile-water（12:88）.The detection wavelength was 240 nm and 250 mm.The flow rate was 1.0mL.min-1.The injection volume was 10 μL.Result:Loganin was linear in the range of 0.31-2.175 μg（r =0.999 9）.Puerarin was a linear in the range of 0.164-1.148 μg（r=0.999 9）.The average recovery of loganin was 100.39% and RSD was 0.72%（n=6）.The average recovery of puerain was 97.99% and RSD was 1.98%（n=6）.Conclusion:This method was simple and specificity with good repeatability,it can be used for the quality control of Anke capsule.关键词：Anke capsule;HPLC;loganin;puerain
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发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for determination of naringin,hesperidin and baicalin in Tongxuan Lifei capsules. Method:The chromatographic column of Agilent ZORBAX SB C18（ 4. 6 mm × 250 mm,5 μm） was used,the mobile phase was consisted of acetonitrile-0. 1% phosphoric acid aqueous solution（ 21:79） .The flow rate was 1. 0 mL· min -1,the detective wavelength was 280 nm and column temperature was 30 ℃ .Result: The linear range of naringin,hesperidin and Baicalin were 0. 156-1. 408 μg（ r=0. 999 8） ,0. 145-1. 301 μg（ r=0. 999 7） and 0. 359-3. 233 μg（ r=0. 999 9） ,the average recoveries （n=9） were 99. 56% （ RSD 0. 88% ） ,were 99. 83% （ RSD 0. 66% ） and 98. 94% （ RSD 1. 55% ） respectively. Conclusion: The results showed thismethod is accurate,reproducible,simple and easy to do,which can be used in quality control of Tongxuan Lifei Capsules.关键词：Tongxuan Lifei capsules;HPLC;naringin;hesperidin;baicalin;content determination
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- 178
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发布时间：2024-09-23 - 摘要：Objective:To establish the HPLC fingerprint analysis methods for Polygonum chinense L and to provide the reference for quality evaluation of Polygonum chinense L.Method:Chromatographic separation was performed on a Platisil ODS C18 column（4.6 mm×250 mn,5 μm） with acetonitrile-0.05% phosphoric acid as a mobile phase in gradient elution for 60min.The flow rate was 1.0 mL·min-1,detected at wavelength of 360 nm.Result:Seventeen chromatographic peaks were determined for characteristic peak constituted a HPLC fingerprint of P.chinense Similarity evaluation results showed that all P.chinense were above 0.90 in similarity.Conclusion:The method is accurate and reliable;the fingerprints can be used for P.chinense quality control.
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发布时间：2024-09-23 - 摘要：目的:建立测定银黄颗粒中黄芩苷的含量测定方法。方法:采用RP-HPLC方法,VP-ODS-C18色谱柱（4.6 mm×150 mm,5μm）,流动相甲醇-0.2%磷酸水（45:55）,流速1.0 mL.min-1,检测波长277 nm。结果:黄芩苷在0.055 6～0.555 6μg,线性关系良好;平均回收率97.6%,RSD 1.37%。结论:方法方便、准确,可作为银黄颗粒质量控制的进一步补充。关键词：银黄颗粒;高效液相色谱法;黄芩苷
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- 129
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发布时间：2024-09-23 - 摘要：Objective:To establish a capillary GC method for the determination of alantoctone and isoalantoctone in Guanxinsuhe capsules.Method:The analytical column was PEG-20M capillary column with anFID detector.The column temperature was programmed from 190 ℃（holding 30 min） at a rate of 120 ℃·min -1 to 240 ℃（holding 5 min）.The injector temperature was 260 ℃.The detector temperature was set at 280 ℃.Result:The linear ranges of alantoctone were 48.96-244.8 mg·L -1,and the linear ranges of isoalantoctone were 51.28-256.4 mg·L -1。Conclution:The methed is sensitive,accurate and reliable,and can be used for quality control of Guanxinsuhe capsules.关键词：GC;Guanxinsuhe capsules;alantolactone;isoalantoctone
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- 127
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发布时间：2024-09-23 - 摘要：目的:建立补肾斑龙片中主要成分人参皂苷Re的含量测定方法。方法:应用高效液相色谱法,以C18柱和乙腈-0.05%磷酸溶液（99∶400）为流动相,检测波长203 nm,流速0.8 mL.min-1,理论塔板数按人参皂苷Re峰计算不低于2 500。结果:标准曲线为Y=17 766 843.51+232 952.03X（r=0.999 8）,人参皂苷Re在2.0～16.0μg成良好线性关系,平均回收率（n=6）97.43%,RSD 1.5%。结论:方法可靠,简单可行。关键词：补肾斑龙片;人参皂苷Re含量;高效液相色谱法
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发布时间：2024-09-23 - 摘要：Objective:To establish the quality standard of Yiganqing Sustained-released-tablet.Method:Hypericum perfortum L,the main ingredient in Yiganqing Sustained-released-tablet was identified by TLC.The content of hypericin in the tablet was determined by using HPLC.The column was Phenomenex Luna C18 column（4.6 mm×250 mm,5 μm）.The mobile phase was acetonitrile-0.025 mol·L-1 KH2PO4 solution（85:15）,and the detection wavelength was at 588 nm.Result:The TLC spots were clear and the separation degree was good.Hypericin was linear in the range of 0.076～0.76（r=0.999 9）.The average recovery was 98.66% and RSD was 2.51%.Conclusion:The results indicate that the method is simple,feasible and repeatable and can be used for quality control of Yiganqing Sustained-released-tablet.关键词：Yiganqing Sustained-released-table;quality standard;TLC;hypericin;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents of Cinnamomum migao.Method:Various chromatographic techniques were employed for isolation and purification of the constituents.Their structures were identified by means of spectral analysis and comparison with the reported data.Result:Compounds isolated from C.migao were identified as（+）-catechin（1）,β-sitosterol（2）,scopoletin（3）,5,7,4’-thihydroxyflavanone（4）,isofraxidin-β-D-glucosid（5）,daucosterol（6）.Conclusion:All compounds were isolated from C.migao for the first time,in which compounds（3-6） were firstly reported in this genus.关键词：Cinnamomum migao;Cinnamomum;chemical constituents
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- 471
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发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for determinating the contents of salidroside and tyrosol in Rhodiola crenulata from Qinghai province,China.Method:All the 25 batches of samples were collected from 2007 to 2009.The HPLC analysis was performed on a C18 column,and methanol-water（9.5:90.5） was as mobile phase and the detection wavelengths were 275 nm.Result:The contents of salidroside and tyrosol were 0.102%1.16% and 0.026% 0.095%,respectively.Conclusion:The contents of salidroside and tyrosol varied from sample to sample.关键词：Rhodiola crenulata;salidroside;tyrosol;content
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- 265
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发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents of Cudrania tricuspidata.Method:The compounds were isolated by column chromatography with silica gel and D-101 macroporous resin.The structures were identified on the basis of spectral analysis.Result:Eight compounds were obtained and identified as lupeol（1）,β-sitosterol（2）,2′,3′-dihydroxy propylpentadecanoate（3）,itesmol（4）,betulin（5）,ursolic acid（6）,sucrose（7） and daucosterol（8）.Conclusion:Compounds 1,3,4,5,6 were obtained from Cudrania genus for the first time,compound 4 was obtained from Moraceae for the first time.关键词：Cudrania genus;Cudrania tricuspidata;chemical constituents
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- 257
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发布时间：2024-09-23 - 摘要：Objective:To develop a GC-MS method to analyze the chemical composition of Huoluo oil.Method:A capillary column HP-5ms UI was used.The column temperature was controlled by a program and the MS analysis was performed with EI and quadrupolemass analyzer.The chemical composition was identified by NIST 2008 searching and mass spectra comparing and their relative contents were calculated by area normalization method.Result:Thirty-three peaks were separated and 30 compounds have been identified,which accounted for over 99% of the peak areas of essential oil.The major components of Huoluo oil were alpha-pinene（8.41%）,eucalyptol（5.59%）,D-camphor（11.7%）,D-menthol（31.31%）,methyl salicylate（25.69%）,anethole（2.83%,）,eugenol（4.24%）,et al.Conclusion:The present study provide scientific bases for exploitation of Huoluo oil.关键词：GC-MS;Huoluo oil;components analysis;Menthol;camphor;methyl salicylate
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- 239
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发布时间：2024-09-23 - 摘要：Objective:To study on the anti-tumor activity of extract and components from Periploca forrestii.Method:The growth-inhibitory effect of extract and components from P.forrestii on tumor cell lines were examined by using MTT assay.Compounds were isolated from the P.forrestii by chromatographic techniques and were identified on the basis of physico-chemical properties and spectral data.Result:Ethyl acetate extract from P.forrestii has strong inhibitory effects against proliferation of K562 tumor cells with the maximum inhibitory of 80.71% at 25 mg·L-1.The component Ⅵ and component Ⅸ has inhibitory effects against proliferation of K562 tumor cells with the maximum inhibitory of 97.48% and 40.56% at 10 mg·L-1.Conclusion:Cell growth in vitro was inhibited significantly by ethyl acetate extract from P.forrestii.Two components have good anti-tumor activity.关键词：Periploca forrestii;chemical composition;anti-tumor
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- 422
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发布时间：2024-09-23 - 摘要：Objective:To study the pharmacognostic characteristics on the stem and leaf of Passiflora edulis.Method:The morphological identification and microscopic identification were adopted.Result:In the cross section of the stem,a few clusters of calcium oxalate were observed in the cortex.Many clusters of calcium oxalate were found in the phloem,with fibers disseminated outside the phloem.Single vessel and 2-3 vessels together were observed in the xylem.The pith was quite wide,equal to 3/4 of the radius of the cross section.In the cross section of the leaf,it could be observed that collateral bundles were interval ring-shaped in the main vein vascular,and some square crystals in the parenchyma cells.Result:Many spiral vessels,fibers,clusters of calcium oxalate and a few square crystals were found in the powder.Conclusion:The plant has obvious pharmacognostic characteristics.These results as above can be used for identification and further application of P.edulis.关键词：Passiflora edulis;morphological identification;microscopic identification
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- 143
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发布时间：2024-09-23 - 摘要：Objective:To find the effective components of Zhuanggu Zhitong prescription,investigate its influence on hormones related to bone metabolism and to explore its possible mechanism.Method:One hundred and fifty SD female rats weighing around 250 g（three months old） were randomly divided into model group（ovariectomized,OVX）,sham operation group,control group,ethanol extraction group A,ethanol extraction group B,petroleum ether extraction A position group,water extraction site group A,water extraction site group B,Fraction extracted with ethyl acetate group A,Zhuanggu Zhitong prescription group,a total of 10 groups（n=15 each）.Five days after stitches of the operation of taking out ovaries,different treatments were carried out for 13 weeks.Blood and bone samples were collected for investigations on bone mineral density,bone biomechanics,pathology and other aspects to evaluate efficacy,and serum estradiol（E2）,bone gla protein（BGP）,parathyroid hormone（PTH）,calcitionin（CT）,to explore the effective mechanism.Result:Compared with model group,Zhuanggu Zhitong prescription group A part of ethanol extraction,water extraction B parts,petroleum ether A site could significantly increase the levels of OVX rats femur,lumbar spine bone mineral density（P<0.05,P<0.01）;could increase osteoporosis in ovariectomized rat tibia model the maximum stress and maximum bending strength（P<0.05,P<0.01）;could significantly increase the average trabecular bone density of the femoral head,the difference was significant（P<0.01）,with no significant difference to Zhuanggu Zhitong prescription group.Compared to OVX model group,Zhuanggu Zhitong prescription could only significantly increase in serum E2,prevent uterine atrophy caused by insufficient estrogen（P<0.05）;Zhuanggu Zhitong prescription could significantly decrease serum BGP,PTH,CT for the castrated rats（P<0.05）.Conclusion:The ethanol extract parts of group A,water extraction site group B,petroleum ether extract A position group site are the effective components of Zhuanggu Zhitong prescription for treating osteoporosis.Zhuanggu Zhitong prescription can increase the serum level of E2,and decrease the levels of BGP,PTH,CT,directly or indirectly regulate the body’s bone metabolism and cytokines,to improve hormone metabolism,the molecular structure of bone matrix and to strengthen the mechanical properties of bone,therefore to treat osteoporosis.关键词：osteoporosis;Zhuanggu Zhitong prescription;effictive components
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发布时间：2024-09-23 - 摘要：Objective:To study the impact of compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis on renal gene expression in SHR rats.Method:Four SHR rat groups were established,including Blank,Gastrodia suspension 0.86 g·kg-1 group,Uncariae Remulus Cum Unicis decoction 1.17 g·kg-1 group,Gastrodiae Rhizoma 0.8 g·kg-1 and Uncariae Ramulus Cum Uncis 1.17 g·kg-1 decoction combined.After 10 days of continuous oral administration,mRNA in the kidney of SHR rats was extracted,and then was hybrided with gene chip.After computerized scanning and analyzing,the changes in gene expression were observed.Result:Thirty target genes before and after compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis were selected out.Conclution:After the compatibility of Gastrodiae Rhizoma with Uncariae Ramulus Cum Uncis,the trends of gene expression related with lipid metabolism,G-protein coupled receptor mediated signaling pathway,and insulin resistance are reversed.It indicates that the related effects of Gastrodiae Rhizoma on kidney are weakened after the compatibility.关键词：Gastrodiae Rhizoma;Uncariae Ramulus Cum Uncis;compatibility;kidney;gene expression
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- 238
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Tangzhiqing（TZQ） on glycolipin metabolism and hepatic histopathology in rabbits with hyperlipoidemia.Method:Rabbit model of hyperlipoidemia was established by feeding high lipid diet.The treatment of TZQ was applied by intragastric administration at dosage of 3.2,1.6,0.8 g·kg-1 respectively for 30 days.The serum content of total cholesterol（TC）,free fatty acid（FFA）,glucose（Glu） and the accumulation of liver triglyeride（TG） and glucogen were determined and the histopathological changes in liver were observed.Result:The serumal content of TC,FFA.Glu and the accumulation of liver TG and glucogen were reduced（P<0.01,P<0.05）,fatty degeneration of the liver were lightened（by rank sum test,P<0.01）in treated group of TZQ.Conclusion:TZQ can regulate the metabolic process of TC,FFA,Glu and decrease the accumulation of liver TG and glucogen,meanwhile has protective effect on hepatic injury in rabbits with hyperlipoidemia.关键词：hyperlipoidemia;Tangzhiqing;glycolipin metabolism;histopathology
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- 151
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发布时间：2024-09-23 - 摘要：Objective:To study the protective effect of chlorogenic acid on liver fibrosis in rats.Method:The rats were randomly divided into 6 groups:the normal control,the model group,the colchicina group（0.2 mg·kg-1）,and the chlorogenic acid groups（140,70,35 mg·kg-1）.Rats in the groups were treated daily for 8 weeks.Model of liver fibrosis induced by carbon tetrachlotide（CCl4） in rats was used.To establish the model,except the rats in the normal control group,the other mice were sc injected with CCl4（5 mL·kg-1） as the first attack and then sc injection of peanut oil of 40% CCl4（3 mL·kg-1） twice a week in the following 8 weeks.The indexes of live and spleen in rats were observed.After blood sample collection,alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities and content of total protein（TP）,albumin（ALB） in serum were examined,meanwhile the ratio of the ALB and globulin（GLOB） was calculated.The four indicators of liver fibrosis hyaluronic acid（HA）,laminin（LN）,procoliagen type Ⅲ（PCⅢ）,collagen type Ⅳ（CⅣ） were determined in liver tissue.Superoxide dismalase（SOD） activity and malondial-dehyde（MDA）,glutalhioneperoxidase（GSH-Px） levels in liver tissue were determined.Result:Chlorogenic acid could obviously decrease the index increase of the live and spleen（P<0.01） in liver fibrosis of rat,remarkably resist the increase of ALT and AST in serum（P<0.01）,increase the content of TP,ALB,A/G in serum（P<0.01）,suppress the content of HA,LN,PCⅢ,CⅣ,GLOB in liver tissue（P<0.01）,decrease the MDA levels in liver tissue（P<0.01）,and improve SOD activity and GSH-Px level in liver tissue（P<0.01）.Conclusion:Chlorogenic acid has significant protective effect on liver fibrosis in rats.关键词：chlorogenic acid;carbon tetrachlotide;liver fibrosis
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- 614
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发布时间：2024-09-23 - 摘要：Objective:To study the influence of Gualougen San on blood glucose and blood lipids in diabetic rats,analyse the relationship between hypoglycemic effect and drug dose.Method:SPF male Wistar rats were injected with 36 mg·kg-1 of STZ intraperitioneally to establish rat models of diabetes.According to the random number table,the model rats were randomly divided into the model group,the metformin hydrochloride group,the Ginseng-astragalus hypoglycemic granule group and the low,middle,high dose（2,4,6 g·kg-1） of Gualougen San groups（n=10 each）.Treatments were ig applied with corresponding drugs every day.Ten normal rats were set up as the normal control group（ig with normal saline）.The fasting blood glucose and weight of the rats were measured before the treatments and eight weeks after the treatments.Blood sample was finally obtained from aorta of rats.Triglyceride and cholesterol were tested.Result:High and low doses of Gualougen San could reduce blood sugar in the diabetic rats（P<0.01）.The low dose of the powder was the strongest while the middle dose of the powder did not show significant effect.High,middle and low doses of Gualougen San all could reduce blood lipids in the diabetic rats（P<0.05）,however,it could not control weight-losing in the rats（P<0.05）.Conclusion:Gualougen San can reduce blood sugar in type 2 diabetic rats without clear dose-effect relationship.The hypolipidemic effect is obvious while it can not change the trend of weight loss in diabetic rats.关键词：Gualougen San;Hypoglycemic effect;Wistar rat;hypolipidemic effect
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of Qifu decoction on adriamycin-induced cardiac apoptosis in rat and reveal possible mechanisms.Method:Fifty-sixe male Sprague Dawley（SD） rats were randomly divided into four groups（the normal group,the model group,Chinese medicine control group and Qifu decoction group with 14 rats in each group）.The normal group:no special treatment,the model group:the rats were treated with ADR 2.5 mg·kg-1,once per week,ip,in the meantime,the rats of the normal group and the model group were given distilled water,10 mL·kg-1,ig.Chinese medicine group:pulse-activating decoction was administrated at the same time of modeling,3.5 mL·kg-1·d-1,ig.Qifu decoction group:Qifu decoction was administrated at the same time of modeling,2.625 g·kg-1·d-1,ig.After 4 weeks,the rats’ body weight were weighed,and then the rats were executed and their heart were weighed,to calculate cardiac index（heart weight /body weight）,Cardiac apoptosis rate was measured by flow cytometry.mRNA expression level of Caspase-3,Bcl-2 and Bax were detected by real time polymerase chain reaction（Realtime quantitative PCR）,to value the radio of Bcl-2/Bax.Result:Adriamycin administration greatly decreased heart weight and caused remarkable cardiac apoptosis.Elevated mRNA expression of Bax and Caspase-3 were obviously observed after Adriamycin treatment,while the elevation was prevented by Qifu decoction.Adriamycin administration reduced the expression level of Bcl-2 mRNA,while Qifu decoction enhanced it.so Bcl-2/Bax ratio was increased by Qifu decoction,all with statistical significance（P<0.05）.Conclusion:Qifu decoction could prevent cardiac apoptosis induced by adriamycin,which might be mediated through the prevention of Bax and Caspase-3 mRNA upregulation and Bcl-2 mRNA downregulation.关键词：Qifu decoction;adriamycin;cardiac apoptosis
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发布时间：2024-09-23 - 摘要：Objective:To investigate effect of Qinyu Burn Liquid（QY） for treatment of burn and scald healing.Method:The burn and scald healing effect of the QY was studied with the scald model of deep Ⅱ degree in rats.Anti-inflammatory effect of the QY was studied with the model of mouse with increase in skinny capillary permeability and the ear swelling induced by xylol in mouse.And,analgesic effect of QY was studied with mouse writhing response and hot plate test.The minimal inhibitory concentration（MIC） and minimal bactericidal concentration（MBC） of QY were determinand by broth dilution method.Result:QY of two doses（crude drugs 0.25 g,0.50 g·mL-1,0.18 mL/times,spray 2 to 3 times a day） had promoting role on rat burn wound healing deepⅡ.QY of two doses（each 20 μL × 4 times,each times interval of 30-60 min） could also obviously inhibit the increase of skin capillaries permeability and swelling of ear in mice induced by xylene,could significantly inhibit twisting caused by acetic acid and pain induced by hot plate in mice,and MIC were respectively 1.95,7.80,15.60 g·L-1 and the MBC were 7.80,15.60,31.25 g·L-1 to Staphylococcus aureus,Pseudomonas aeruginosa and Escherchia coli（E.coli）.Conclusion:On one hand the wound heal time of QY for rats with Ⅱ degree deep scald is superior to that of the model group.On the other hand,QY also has significant analgesic,anti-inflammatory and effect on S.aureus,P.aeruginosa and E.coli,especially S.aureus.关键词：Qinyu Burn Liquid;burn and scald;analgesic;anti-inflammatory;bactriostasis
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- 328
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发布时间：2024-09-23 - 摘要：Objective:To study the mechanisms of ameliorating insulin resistance（IR） by astragalus polysaccharides（APS） in rats with IR.Method:Fourty-eight healthy male Sprague-Dawley rats were randomly divided into two groups:the normal control group（NC group,n=12） and the high fat-diet-induced model group of IR（HFM group,n=36）.Rats in the NC group were fed with ordinary diet while those in the HFM group were fed with high fat diet for six weeks.Then the rats in the HFM group were randomly divided into three groups:the high fat-diet-induced control group（HFC group,n=12）,pioglitazone group（Pio group,n=12）,APS group（n=12）,lavaged with saline,pioglitazone（20 mg·kg-1·d-1） and APS（200 mg·kg-1·d-1） respectively for eight weeks.Changes in fasting blood glucose（FBG）,fasting insulin（FINS）,free fat acid（FFA）,resistin and adiponectin of rats were routinely measured,meanwhile glucose infussion rate of tissue was evaluated by hyperinsulinaemic-euglycaemic clamp technique.Result:The levels of plasma FINS,FFA,resistin of rats were significantly higher and the levels of APN,glucose inffusion rate of tissues were significantly lower in the HFC group than those in the NC group（P<0.05）.Compared with those in the HFC group,the above mentioned indexes in APS group were improved significantly（P<0.05）.GIR and the levels of FFA,FINS,resistin,APN were conducted linear correlation analysis and showed significant correlations.Conclusion:The results indicate that APS can regulate part of the insulin signaling in IR serum,and that APS could be a potential insulin sensitizer for the treatment of IR by an increase of adiponectin and reduction of resistin in serum.关键词：astragalus polysaccharides;hyperinsulinaemic-euglycaemic clamp technique;insulin resistance;adiponectin;resistin
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发布时间：2024-09-23 - 摘要：Objective:To analysis pharmacodynamic contributed values of composition and pharmacodynamic simulation of compound Hugu capsules（HG）.Method:Ten components of HG were divided into 13 groups（group 13 for negative control group） by orthogonal simulation method,the drug serum was gotten by the serum pharmacological experiments and CCK-8 method was adopted to detect osteoblast（Ob） proliferation.Pharmacodynamic contributed values and pharmacodynamic simulation of compound were analysis by DAS3.0 software.Result:The proliferation of Ob as a index,a sequence of pharmacodynamic contributed values were found by orthogonal simulation method:Epimedii Folium>Drynariae Rhizoma>Morindae Officinalis Radix>Dioscoreae Rhizoma>Testudinis Carapax et Plastrum>Angelica Sinensis Radix>Rehmanniae Radix Praeparata>Dipsaci Radix>Eucommiae Cortex>Polygoni Multiflori Radix Praeparata Multiflorum F.With Ob proliferation as the index,and the results of pharmacodynamic simulation of compound showed that 27 compounds were better than the negative control group,and the original compound is the best in effect,the other four compounds are close to the original compound.Conclusion:Orthogonal simulation method is a method to analyze the Chinese herbal compound.This approach can determine pharmacodynamic contributed values of composition,and pharmacodynamic simulation of compound for HG.The method can provide a lot of information for studying Chinese herbal compound,and is benefit for developing new drug.关键词：orthogonal simulation method;Hugu capsules;Osteoblast;pharmacodynamic contributed values;simulation efficacy
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发布时间：2024-09-23 -
Protective Effect of Kangdu Buxin Capsule on Chronic Myocardium Injury Induced by Adriamycin in Rats
摘要：Objective:To investigate the protective effect of Kangdu Buxin Capsule on chronic injury and cardiotoxicity induced by adriamycin（ADM） in rats.Method:Male SD rats were randomly divided into six groups.Besides the control group,each group were injected with adriamycin（ADM,3 mg·kg-1 peritoneal injection） weekly for 6 weeks.Meanwhile,4 ADM-injected groups were received the treatment of Kangdu Buxin Capsule（0.4,0.8,1.6 g·kg-1） and Fufang Danshen tablet（0.45 g·kg-1）,respectively.Survivors were observed for hemodynamics changes.LVEF,FS and B-type Natriretic peptide（BNP）,the activities of GSH-Px and SOD,and MDA were measured and the hearts were removed for histological examination by light microscopy.Result:LVEF and FS were increased compared with the injury group,the activities of GSH-Px and SOD were higher,and the MDA lever was lower in therapy groups（P<0.05）.Conclusion:Kangdu Buxin Capsule has protective effect and antioxidative effect on the cardiomyopathy of chronic heart disease induced by ADM in rats.关键词：Kangdu Buxin Capsule;adriamycin;antioxidative effect;myocardial protection- 0
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the antianxiety effect of Coptidis Rhizoma in mice and study the related mechanism.Method:Male mice were randomly divided into 5 groups（n=12 each,model control group,the positive control group,1.8 g·kg-1 high-dose group Coptidis Rhizoma decoction,the 0.9 g·kg-1 dose,0.45 g·kg-1 low-dose group）.Model group was given normal saline,and the positive group was given 2.5 mg·kg-1 diazepam.Mice were treated with drugs for 5 days.We carried out the elevated plus-maze test and light-dark transitions test half one hour after the last drug administration.HPLC-FLD was applied to detect the content of gama-aminobutyric acid（GABA）,glucose（Glu） and 5-hydroxytryptamine（5-HT） in mouse’s brain.Result:Coptidis Rhizoma decoction could significantly increase the percentage of the times entering the open arms and stay duration in the total period.And it could increase the times of passing the box.The content of GABA in Coptidis Rhizoma high dose group was the highest,and GABA in positive group was more than that in the model group.Conclusion:Coptidis Rhizoma displays anti-anxiety effect in the elevated plus-maze test and light-dark transitions test.The mechanism is related with enhancing the content of GABA in the brain.关键词：Coptidis Rhizoma;antianxiety;gama aminobutyric acid;HPLC-FLD
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the acute toxicity and the protective effects of the extraction from caulis of Schisandra chinensis on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.Method:The maximum dosage（concentration 0.40 g·mL-1,dose 0.04 mL·g-1） of the extraction from caulis of S.chinensis was used as ig administration in mice to measure the maximum tolerance dose（MTD）.With different doses of the extracts（ig 3.03,4.55,9.09 g·kg-1）,the levels of ALT and AST in serum were observed for acute liver injury induced by CCl4 in mice.Result:The MTD of the extraction in mice was 32 g·kg-1·d-1.All dose groups of the extraction from caulis of S.chinensis could decrease the levels of ALT and AST in serum.Compared with the model control group,moderate and high dose groups could significantly decrease the levels of ALT and AST in serum（P<0.05,P<0.01）.Conclusion:The extraction from caulis of S.chinensin has low toxicity,and shows protective effect on acute liver injury induced by CCl4 in mice.关键词：caulis of Schisandra chinensin;acute toxicity;liver injury
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发布时间：2024-09-23 - 摘要：Objective:To investigate the influences of compatibility of Magnoliae Officinalis Cortex with Aurantii Frutus Immaturus on gastric motility in rat.Method:The gastric motility disorder in SD rats was established by injecting L-Arg intraperitoneally for six days,then the rats were given the extract of Magnolia（Magnoliae Officinalis Cortex 10 g·kg-1） by intragastric administration,the compatibility of extract of Magnoliae Officinalis Cortex with Frutus auranti（Magnoliae Officinalis Cortex and Aurantii Frutus Immaturus 20 g·kg-1） by intragastric administration,cisapride（0.004 2 g·kg-1） by intraperitoneal injection,respectively.The improvement in gastric function was observed.Result:Gastrointestinal motility in rats injected with L-Arg intraperitoneally was slowed down,plasma motilin was reduced.The extract of Magnoliae Officinalis Cortex,Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus could effectively inhibit the decrease in gastrointestinal motility（P<0.05） and plasma motilin（P<0.05）.and there was significant difference between the group Magnoliae Officinalis Cortex and the group Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus（P<0.05） and there was no significant difference between Magnoliae Officinalis Cortex and the positive drug cisapride.There were significant differences between the group Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus compared with the group Magnoliae Officinalis Cortex,and the group of compatibility compared with cisapride（P<0.05）.Gastric residual rate in rats given the extract of Magnoliae Officinalis Cortex,the extract of Magnoliae Officinalis Cortex compatibility with Frutus auranti,cisapride were（69.3±13.7）%,（60.7±12.9）% and（70.1±12.9）% respectively;plasma motilin were（85.4±11.2）,（93.1±10.4） and（86.3±12.4）nmol·L-1 accordingly.Gastric residual rate was lower（P<0.05） and plasma motilin was higher（P<0.05） for the decoction duration of 90 min compared with those of duration 60 min or 30 min.Gastric residual rate for the decoction duration 90,60,30 min were（47.7±12.8）%,（59.8±12.1）% and（62.7±11.8）% respectively;plasma motilin were（102.3±10.6）,（92.9±10.1） and（89.6±11.2） nmol·L-1 respectively.Conclusion:The extract of Magnoliae Officinalis Cortex,Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus can effectively improve the gastric motility disorder in rats,and Magnoliae Officinalis Cortex compatibility with Aurantii Frutus Immaturus is better than Magnoliae Officinalis Cortex alone.The recommended decoction duration is 90 min.关键词：Magnoliae Officinalis Cortex;Aurantii Frutus Immaturus;compatibility;gastric motility disorder
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发布时间：2024-09-23 - 摘要：目的:探讨益肾活血方含药血清对低糖低氧脑损伤大鼠海马神经干细胞增殖的影响。方法:从新生Wistar大鼠海马分离、培养神经干细胞（NSCs）,将培养出的NSCs在95%N2,5%CO2,37℃培养6 h,低糖低氧体外模拟脑缺血损伤过程。随机分为3组,给药组给予益肾活血方15%含药血清;模型组给予正常大鼠未给药血清,体积分数为15%。正常组采用常规NSCs培养方法。各组细胞在37℃,5%CO2培养1 d后,运用MTT法、克隆计数观察损伤后NSCs的增殖情况。结果:低糖低氧脑损伤（低糖低氧损伤）后部分NSCs死亡,NSCs克隆数为（689.2±27.8）个,益肾活血方含药血清加入后克隆增大,克隆数增多,其中给药组为（738.6±26.6）个,2组比较差异有显著性（P<0.05）。MTT检测吸光度（A）模型组为0.617±0.21,给药组为0.895±0.37,2组比较差异有显著性（P<0.05）。结论:益肾活血方含药血清可促进低糖低氧脑损伤NSCs的增殖。关键词：益肾活血方含药血清;低糖低氧脑损伤;神经干细胞;增殖
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发布时间：2024-09-23 - 摘要：Objective:To probe the mechanism of Honghua Ruyi Pill for treatment of chronic pelvic inflammation through observing inflammatory cytokine in serum and immune molecules relative to metrosynizesis.Method:The animal model of chronic pelvic inflammation was replicated by injection of phenol mucilage.After 10 days,the model was replicated successfully.The rats were divided into six groups randomly,normal group,model group,lower dosage of Honghua Ruyi Pill（0.156 g·kg-1）,middle dosage of Honghua Ruyi Pill group（0.469 g·kg-1）,large dosage of of Honghua Ruyi Pill group（1.407 g·kg-1）and Cinnamon twig and Poria capsule group（0.326 g·kg-1）.After the rats were treated 20 days with corresponding medicine by intragastric administration,the pathological of endometrium was observed and the concentrations of tumor necrosis factor（TNF-α）,interleukin-2（IL-2）and intercellular adhesion molecular（ICAM-1） were examined in serum by enzyme linked immunosorbent assay（ELISA）.Result:Honghua Ruyi Pill could alleviate obviously the pathological damage of endometrium.In comparison with normal group,serum IL-2 in model group was significantly decreased（P<0.01）,and TNF-α was significantly elevated（P<0.01）;the concentration of ICAM-1 in uterus was significantly elevated（P<0.01）.After Honghua Ruyi Pill（1.407 g·kg-1）treatment,serum IL-2 was obviously elevated compared with the model group and TNF-α was obviously decreased（P<0.05）;the concentration of ICAM-1 in uterus was significantly decreased（P<0.05）.Conclusion:Honghua Ruyi Pill can alleviate the inflammation and adhesion of pelvic by regulating the expression of inflammatory cytokines in serum and intercellular adhesion molecules in endometrium.关键词：pelvic inflammatory disease;cytokine;cell adhesion molecules
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发布时间：2024-09-23 - 摘要：目的:观察黄芪破壁粉粒对气虚动物模型的影响,同时进行急性毒性实验以观察黄芪破壁粉粒的安全性,并与黄芪饮片进行比较。方法:采用大黄ig加限制饮食法复制小鼠气虚动物模型,观察黄芪破壁粉粒对气虚动物红细胞计数、血红蛋白含量以及负重游泳时间的影响。取KM种小鼠,随机分为正常对照组,模型对照组,黄芪饮片组（2.0 g.kg-1）,生脉胶囊组(0.9 g.kg-1和黄芪破壁粉粒2.0,1.0,0.5,0.25 g.kg-14个剂量组。ig给药,连续10 d;采用最大给药量法（MTD）观察黄芪破壁粉粒的毒性。KM种小鼠60只随机分为正常对照组,黄芪破壁粉粒组和黄芪饮片组,每组各20只。然后以小鼠能耐受的最大浓度（每毫升含黄芪破壁粉粒0.4 g.mL-1）、最大容积（0.04 mL.g-1体重）ig给药2次,每隔6 h 1次。结果:黄芪破壁粉粒能够显著增加气虚动物红细胞数量,模型组（10.33±0.72）×1012/L,黄芪破壁粉粒组（11.30±0.63）×1012/L;亦能增加血红蛋白含量,模型组（15.55±0.67）g/L,黄芪破壁粉粒组（16.67±1.03）g/L;延长负重游泳时间,模型组（90.50±13.13）s,黄芪破壁粉粒组（132.80±14.90）s;黄芪破壁粉粒1日2次给药的最大给药量为32 g.kg-1体重,相当于原药材临床用量的64倍。结论:黄芪破壁粉粒对气虚证动物模型有改善作用,黄芪破壁粉粒、黄芪饮片最大给药量均为32 g.kg-1,未见对小鼠产生明显毒性反应。关键词：黄芪;破壁粉粒;气虚动物;急性毒性
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the effects of Shaofu Zhuyu decoction,the decoction minus Wenli herb pair（herbs of warm channel and expelling cold） on hemorheological parameters in rat mode of acute blood stasis,and the influence on platelet aggregation and clotting time in vitro.Method:Rat model of acute blood stasis was used to evaluate the changes in hemorheological parameters,the function of blood coagulation and ovarian.The platelet aggregation was tested with Born’s method.The clotting parameters,including thrombin time（TT）,prothrombin time（PT） and activated partial thromboplastin time（APTT） in vitro,were established.Result:Shaofu Zhuyu decoction showed significant improvements in the hemorheological parameters,including whole blood viscosity and ESR（P<0.05） compared with the model of blood stasis.Meanwhile,the result of vitro assay showed that Shaofu Zhuyu decoction had significantly inhibitory effects on the platelet aggregation and prolonged clotting time for TT,PT and APTT.The effects of the decoction minus Wenli herb pair only showed effect of reducing plasma viscosity and extending TT and PT.Conclusion:Shaofu Zhuyu decoction could improve the indexes of hemorheology in rat model of blood stasis,and showed significant inhibitory effect on platelet aggregation and clotting time.Furthermore,Wenli herbs play an important role in the biological effect for the whole prescription.关键词：Shaofu Zhuyu Decoction;herbs of warm channel and expelling cold;activating blood circulation to dissipate blood stasis
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Kangdu Buxin Capsule on immune function in immuno-suppressed（IS）mice induced with cyclophosphamide（CTX）.Method:The IS model was produced by intraperitoneal injection（ip）of CTX with a dose of 50 mg·kg-1in mice.The mice were randomly divided into normal control group,immunity deficiency model group,Kangdu Buxin Capsule treated group and positive control group respectively.The changes in the number of WBC,the phagocytosis activity of macrophage,and the proliferation of T lymphocyte in the spleen of mice were observed.Result:Compared with model group,Kangdu Buxin Capsule could increase the amount of the peripheral blood WBC（P<0.05）,the phagocytosis activity of macrophage,and the proliferation of T lymphocyte compared with the model group（P<0.05）.Conclusion:Kangdu Buxin Capsule has the effect of improving immune in immuno-suppressed mice induced with CTX,and has protective effect on the immune function of mice.关键词：Kangdu Buxin Capsule;cyclophosphamide;immune
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of Naopingkang granule on learning and memory in rats after transient cerebral ischemia.Method:Sixty Wistar rats（n=10/group） were randomly divided into six groups:the normal goup,the model group,the Donepezil group,high Naopingkang granule group（12 g·kg-1）,middle Naopingkang granule group（6 g·kg-1） and low Naopingkang granule group（3 g·kg-1）.The treament groups were orally administered once per day separately,whereas the normal and model group were orally administered with distilled water.Ischemia model was made with 2-vessel-occlusion+hypotension,Morris water maze test and step-through passive avoidance test were employed to investigate the influences of Naopingkang granule on transient cerebral ischemia in rats.Result:In Morris water maze test,Naopingkang granule could significantly decrease escape latency time,swimming times in target quadrant was significantly recovered compared with that of model group（P<0.05）;in Step-through passive avoidance test,the shorter step-through latency and much error number induced in transient cerebral ischemia was significantly reversed by Naopingkang granule treatment compared with that of model group（P<0.05）;activety of AchE was inhibited compared with that of model group（P<0.05）.Conclusion:Naopingkang granule could improve learning and memory.关键词：Naopingkang granule;transient cerebral ischemia;learning and memory
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发布时间：2024-09-23 - 摘要：Objective:To investigate the sedation,syngignoscism and anticonvulsant actions of different Fluoritum with different colour.Method:The acute toxicity of Fluoritum with different colour was investigated by mice acute toxicity test.The sedation,syngignoscism and anticonvulsant actionss of the Fluoritum（20 g·kg-1,ig,3 d）were evaluated by testing and observing independent activity,sleep time and convulsion caused by pentrazole in mice.Result:It was not seen the acute toxicity in mice treated by Fluoritum with different colour.Mice treated with Fluoritum reduced the time of independent activities without significant difference.All the Fluoritum with different colour could extend the sleep time of mice with threshold dose of pentobarbital sodium obviously,and showed synergistic effect with pentobarbital sodium.Among them,the 23#,25# showed the obvious differences（P<0.05 or P<0.01）.Fluoritum with different colour did not influenced the time of convulsion of mice significantly.There were no obvious difference and no extension on the elapsed time of convulsion as well.Conclusion:It is not seen the acute toxicity of mice treated with treated Fluoritum with different colour.Fluoritum can reduce the times of independent activities of mice,and extend the sleep time of mice treated with pentobarbital sodium,there for,has synergistic effect with pentobarbital sodium.It has no obvious impact on both the time and the amount of time of convulsion.So,Fluoritum shows sedative action.Fluoritum with different colour shows no significant diffirence.关键词：fluoritum;sedation;convulsion
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of Guizhi Fuling Capsule on expression of monocyte chemoattractant protein-1（MCP-1） and inter-cellular adhesion molecule-1（ICAM-1） mRNA of ectopic endometrium in rats with endometriosis.Method:Endometriosis model of rat was created by self-transplant,and then administrated with Guizhi Fuling Capsule（0.256,1.024 g·kg-1） orally for 28 days.Expression and distribution of MCP-1 and ICAM-1 in ectopic endometrium were observed by immunohistochemistry.The mRNA expression of MCP-1,ICAM-1 were evaluated with RT-PCR.Result:The expression and distribution of MCP-1 and ICAM-1 were significantly decreased after treatment with Guizhi Fuling Capsule.Both dose showed similar effect without significant difference.Conclution:The distribution of MCP-1 and ICAM-1 in ectopic endometrium of endometriosis can be modified by Guizhi Fuling Capsule and the mRNA expression was decreased.关键词：Guizhi Fuling Capsule;endometriosis;ectopic endometrium;MCP-1;ICAM-1
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发布时间：2024-09-23 - 摘要：Objective:To study the protective effects of Modified Xionggui Decoction（MXD） against global cerebral ischemia-reperfusion injury（GCIRI） in rats.Method:According to Pulsinelli’s method,bilateral vertebral artery of rats was occlusion by electrocautery,followed by blocking blood flow of bilateral common carotid artery for 10 min,then reperfusion as GCIRI model.Ninety-six Sprague Dawley（SD） rats were randomly divided into 6 groups,one of them was sham-operation control group,and the others were GCIRI models.Three doses of MXD（0.38,0.76,1.52 g·kg-1） and Tongmai Granule（2.7 g·kg-1） were administered intragastrically respectively for 9 days,and water was used instead of drugs in Sham-operation and model control groups.The rats were pre-treatment of drug for 6 days before preparing GCIRI model and post-treatment for 3 days after establishment of GCIRI model.Neurological severity scores（NSSs） in the rats were observed 3 d after reperfusion.At the 4th day after reperfusion,the water content of the cerebal tissue was determined by weighing method,and concentration of malondialdehyde（MDA） were measured by TBA method in the plasma and the cerebal tissue,and activities of superoxide dismutase（SOD）,creatine kinase（CK） and lactate dehydrogenase（LDH） were assayed by spectrophotometry,and caspase-3 was tested by immunohistochemistry.Pathology of the brain were observed under microscope.Result:Compared with the model group,the brain water content,the NSS,the concentration of MDA and the expression of neurocyte caspase-3 in the pallium were degraded in MXD group.The activity of SOD,CK and LDH was increased in MXD group（P<0.05）.Conclusion:MXD has obvious protective effects against cerebal IR injury.关键词：Xionggui Decoction;ischemia-reperfusion;injury of brain;protective effect
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of panax notoginseng saponins（PNS） on cerebral ischemia reperfusion injury in rats.Method:SD rats were divided into sham operation group,model group,PNS group.Model group and PNS group were established ischemia-reperfusion injury by using ligation of middle cerebral artery.PNS was given 15 min before ischemia and 6 h after ischemia,respectively,ip PNS 100 mg·kg-1 for rats in PNS group.Rats in model group and sham group were given at the same time ip with normal saline.The neurologic deficit score,brain infarction size and the levels of interleukin-1β（IL-1β）,tumor meerosis factor-α（TNF-α）,interleukin-6（IL-6）,interleukin-8（IL-8） and content of Evans blue in brain tissue were examined.Result:PNS could reduce infarct volume of cerebral ischemia-reperfusion injury in rats,reduce the extent of the damage in blood-brain barrier significantly.The levels of cytokines and content of Evans blue in PNS group were successively decreased as compared with those in model group（P<0.05）.Conclusion:PNS has a significant brain protection on ischemia-reperfusion injury.PNS has significant effect of reducing the levels of cytokines,PNS can also reduce the permeability of blood-brain barrier.关键词：panax notoginseng saponins;brain ischemia-reperfusion injury;cytokines;blood-brain barrier
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发布时间：2024-09-23 - 摘要：Objective:To investigate the mechanism of reducing renal interstitial fibrosis of chronic pyelonephritis with Erding-erxian Decoction by adjusting estrogen.Method:Fifty SD female rats were made into castrated chronic pyelonephritis models by cutting both sides of ovaries and putting Escherichla coli to incompletely obstructed ureter from bladder.They were randomized into 5 groups,model group,norfloxacin with premarin group（0.875 g·kg-1+0.044 g·kg-1）,Erding Decoction group（25 g·kg-1）,Erxian Decoction group（35.4 g·kg-1）,Erding-erxian Decoction group（60.4 g·kg-1）.And other 10 normal SD female rats were raised in the same period of time.The rats in each group were fed different relative drugs by intragastric administration once every day for six weeks.Urine,blood and renal tissues were taken after the treatment,while the urine regular test,urinary protein,urinary β2 microglobulin β2-MG,plasma estrogen were tested and renal pathological change were observed including transforming growth factor-β（TGF-β1） and α-smooth muscle actin（α-SMA） using immunochemistry staining method.Result:Erding-erxian Decoction could greatly improve the estrogen of castrated female rats suffering from chronic pyelonephritis.The effect was better than other groups.It could be an important mechanism of reducing TGF-β1 and α-SMA of the renal tissues so as to relieve renal interstitial fibrosis.Conclusion:Erding-erxian Decoction could relieve renal interstitial fibrosis in castrated female rats suffering from chronic pyelonephritis.关键词：Erding-erxian Decoction;chronic pyelonephritis;renal interstitial fibrosis;estrogen
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发布时间：2024-09-23 - 摘要：Objective:To determine the effects of combined treatment of ligustrazine with puerarin on nerve growth factor（NGF） level in rats with cerebral ischemia injury.Method:Rats were randomized into sham-operation group,model group,ligustrazine-puerarin group（50 mg·kg-1for both） and nimodipine group（0.5 mg·kg-1）.The four vessel occlusion method was used to establish the model.The level changes of NGF in brain and the changes of nerve function were investigated.Result:Compared with sham-operated group,in model group rats,the level of NGF in brain was decreased significantly;compared with model group the neuronal damage and inflammatory reactions were alleviated after the treatment,the content of NGF in brain was increased,and nerve function was improved.Conclusion:The combined treatment of ligustrazine with puerarin can prevent and cure the ischemia injury to some degrees.关键词：ligustrazine;puerarin;cerebral ischemia;rat;NGF
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发布时间：2024-09-23 - 摘要：目的:观察补肝方对亚健康大鼠模型下丘脑单胺类神经递质含量及海马脑区谷氨酸受体（NR2A mRNA）表达的影响。方法:采用游泳运动、睡眠剥夺和夹尾刺激等复合因素制造亚健康大鼠模型,分别以生理盐水、补肝方高、低剂量（含生药20,10 g.kg-1）ig,1次/d,连续20 d,检测下丘脑多巴胺（DA）,5-羟色胺（5-HT）含量及海马脑区NR2A mRNA表达。结果:模型组下丘脑DA含量明显低于正常组（P<0.01）,补肝方高、低剂量组下丘脑DA含量均高于模型组（P<0.01）。模型组下丘脑5-HT含量较正常组明显增高（P<0.01）;补肝方高、低剂量组比模型组有明显降低（P<0.01）。模型组NR2A mRNA表达明显低于对照组（P<0.01）,补肝方有升高NR2A mRNA的趋势。结论:补肝法改善疲劳、烦躁、失眠等表现的机制可能与调节DA和5-HT的异常,上调海马NR2A mRNA的表达,抑制兴奋性氨基酸类神经递质的传导异常有关。关键词：补肝方;亚健康;多巴胺;5-羟色胺;谷氨酸受体基因
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- 269
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发布时间：2024-09-23 - 摘要：Objective:To detect the expression of nerve growth factor（NGF） in glandula prostata of chronic prostatitis（CP） rat model treated with Qianlie Shutong capsule.Method:The specific primers and probes for Taqman real-time PCR was disigned according to the NGF and GAPDH cDNA sequence.The expression level of NGF as measured by relative quantification real-time PCR in rat model treated with Qianlie Shutong capsule 0.84,0.42,0.21 g·kg-1,ig for 30 days.Result:Treatment with Qianlieshutong decreased the expression of NGF in glandula prostata of rat model with CP significantly compared to the control group.Conclusion:The expression levels of NGF was degraded in CP rat model treated with Qianlieshutong.关键词：chronic prostatitis;Qianlie Shutong capsule;NGF
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发布时间：2024-09-23 - 摘要：目的:观察黄芪桂枝五物颗粒对急性脑损伤光化学诱导脑血栓形成大鼠梗死面积、血管损伤区、血小板聚集及血浆组织纤溶酶原激活剂（t-PA）等各项指标的影响,探讨其治疗脑血管疾病的作用机制。方法:对光化学诱导脑血栓形成大鼠给予黄芪桂枝五物颗粒0.828,0.414,0.207 g.kg-1ig 15 d,以龙芪溶栓胶囊（0.162 g.kg-1）为阳性药,观察对模型动物脑梗死面积、血管损伤区、血小板聚集及血浆t-PA等各项指标的影响,评定其对该模型的治疗功效。结果:黄芪桂枝五物颗粒能明显减少模型大鼠的脑梗死面积,抑制血小板聚集,并维持纤维蛋白原含量在较高水平。结论:黄芪桂枝五物颗粒0.828,0.414 g.kg-1口服2周对于光化学诱导脑血栓形成大鼠脑梗死的发生有明显的抑制作用,对于血管损伤的发生也有一定抑制作用。关键词：黄芪桂枝五物颗粒;光化学;脑血栓
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发布时间：2024-09-23 - 摘要：目的:研究美乐涂膜剂及其拆方促进皮肤愈合的作用,为临床应用提供依据。方法:高温水烫伤复制深Ⅱ度烫伤模型,观察美乐涂膜剂全方及拆方对皮肤愈合的影响;二甲苯致炎复制耳肿胀模型,观察美乐涂膜剂对耳肿胀的影响;采用最大给药量法,观察急性毒性。采用多次给药法,观察皮肤刺激性。结果:美乐涂膜剂全方9.92,4.96 g.kg-1、中药组分9.88,4.94 g.kg-1组和化学药组分0.06 g.kg-1组能明显使烫伤后创面愈合率增加,全方和各拆方均能缩短烫伤皮肤愈合时间;全方5.6,2.8 g.kg-1组能明显抑制二甲苯引起的小鼠耳肿胀;未见急性毒性反应;未见皮肤刺激性。结论:美乐涂膜剂具有抗炎、促进皮肤愈合作用,全方较单独中药组分、西药组分疗效稍好,临床可安全用药。关键词：美乐涂膜剂;拆方;抗炎;急性毒性;刺激性
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发布时间：2024-09-23 - 摘要：Objective:To prove Gongdiwan’s function of improving immune system,and inhibiting bacterium through pharmacodynamics study.Method:①Study on Gongdiwan’s function of inhibiting bacterium in vitro through slotted method;②Study on Gongdiwan’s influence to mice’s function of Carbon clearance:select 50 ICR mice,male,which were randomized to control group,Liuwei Dihuang group（dosage:2 gram crude drug per kilogram）,Gongdiwan high dosage,mild dosage and low dosage group（18 grams crude drug per kilogram,9 grams crude drug per kilogram,4.5 grams crude drug per kilogram）.③Gongdiwan’s influence on normal mice’s delayed type hypersensitivity,select 50 ICR mice,male,weighting between 18-22 grams,which were randomized to 5 groups,each group has 10 mice,the grouping method ditto.Result:Gongdiwan had no function of inhibiting staphylococcus aureus and proteus,but it could inhibit beta hemolytic streptococcus and escherichia coil in vitro.The result showed that the inhibiting bacterium function of Gongdiwan was weaker then that of western medicine group.Comparing with control group,Gongdiwan could obviously improve the mice’s function of Carbon clearance,especially when using low dose（P<0.01）,that was to say,Gongdiwan could improve the non-specific immune function of mice.Comparing with control group,the low dose Gongdiwan Group could obviously enhance the DNCB induced mice’s delayed type hypersensitivity（P<0.05）.Conclusion:Gongdiwan can inhibit bacterium and improve the function of immune system.Therefore,it can effectively treat urinary tract infection（stranguria caused by overstrain） that belong to spleen and kidney yang deficiency syndrome,which are difficult to be cured by western medicine.Thus,Gongdiwan can treat the catabasis of chronic urinary tract infection.关键词：urinary tract infection;Gongdiwan;pharmacodynamics
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发布时间：2024-09-23 - 摘要：Objective:To study the cellular growth of cell suspension culture and kinetics of product accumulation in Siraitia grosvenorii,and provide a theoretical basis for optimal control in the training process.Method:The fresh weight,dry weight and the contents of mogroside were investigated in a cell cycle,the growth curve and growth rate curves were drawn.Result:The growth cycle of S.grosvenorii cell suspension culture was 21 days,the lag phase was 0-6 d,the log phase was 7-21 d,there was transient stability in the stable phase,the decline phase achieved on the 22th day.The highest fresh weight and dry weight were achieved on the 21th day,and reached 693.0,416.0 g·L-1respectively,the maximum growth rate reached 0.040 g·L-1·d-1.The mogroside and mogroside Ⅴsynthesize rapidly on the 7th day,the highest growth rate of mogroside and mogroside Ⅴ were achieved on the 21th day,and reached 0.032 8,0.054 7 g·L-1·d-1 respectively.Conclusion:The combined study of cellular growth and product accumulation can figure out the dynamic changes of product accumulation,and provide a theoretical basis for optimal control in the training process,regulation of metabolism and large-scale industrialization in S.grosvenorii.关键词：Siraitia grosvenorii;cell suspension culture;cellular growth;mogroside;kinetics
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发布时间：2024-09-23 - 摘要：Objective:To observe the safety on oil concoction of Sanhuangpian.Method:Acute toxicity test was made in 40 mice skin by smearing individually oil（0.2 mL per mice）on the bare skin of mice back for control group and the oil concoction of Sanhuangpian（0.4 g per mice）for treatment group.At same time we have made the skin irritation test through in 20 guinea pigs skin by smearing the oil concoction of Sanhuangpian（1.0 g per guinea pig）on the left bare skin of guinea pig back and oil（1.0 mL per guinea pig）on that of the right.Then the skin allergic test was made on 30 skin of guinea pig by smearing oil concoction of Sanhuangpian（0.5 g per guinea pig）on the bare skin of guinea pig back for treatment group,with oil（0.5 mL per guinea pig）and 2,4-dinitrochlorobenzene（0.5 mL guinea pig） respectively for control group.Result:The external application of Sanhuangpian oil concoction has no significant toxicity on skin,there was no animal death in the observing continuously of 7 days after using of Sanhuangpian oil concoction,theiy activities were not abnormal.There is no significant irritation on skin,no erythema of guinea pig skin,edema,allergic reactions,sensitization rates were 10%,0,there is no skin irritation obvious allergic reactions.Conclusion:The oil concoction of Sanhuangpian has definite safety on clinical application,a more widespread use should been made to people.
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术期刊光盘版"全文收录期刊;并被评为"中国中医药优秀期刊"及"中国学术期刊优秀期刊"。本刊创刊于1995年10月,本着提高为主,提高与普及相结合的办刊方针,设置:工艺与制剂、化学与分析、药理、临床、综
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发布时间：2024-09-23 - 摘要：Objective:To observe Duhuo Jisheng decoction for treatment of lumbar disc herniation patients.Method:Sixty eight patients were randomly divided into 2 groups.The treatment group with 34 patients was treated by Duhuo Jisheng Decoction.The control group with 34 patients was treated with diclofenac sodium enteric-coated troche.Seven days was a course of treatment,an average of 3 courses of treatment was observed,clinical signs were observed.Result:The total effective rate was 88.24% for treatment group（30 cases）,the control group was 64.71%（22 cases）,2 groups were found significant difference（P<0.05）.The course of the two drugs had no obvious adverse reactions.Conclusion:Duhuo Jisheng decoction for treatment of lumbar disc herniation has a significant effect.关键词：Duhuo Jisheng decoction;lumbar disc herniatio;treatment effect
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- 378
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of combined Zhimi Lingshuan and Xinxibao therapy in the treatment of chronic cervicitis and in the prevention of cervical carcinoma.Method:One thousand six handreds and sixty eight patients with chronic cervicitis were divided into two groups randomly.One was the test group,the patients were accepted combined Zhimi Lingshuan and Xinxibao therapy.The other was the control group,the patients were accepted micro-wave therapy.The curative effects were checked after 3 months for the two groups.All the patients were followed up for 36 months for cytologic examinations of cervical scraping smear and cervical/endocervical biopsy was applied if necessary.Result:Both the curative rate and the clinical total effective rate in the test group was higher than that in the control group（P<0.05）while the incidence rate of cervical carcinoma in the test group was significantly lower than that in the control group（P<0.05）.Conclusion:Combined Zhimi Lingshuan and Xinxibao therapy can effectively relieve chronic cervicitis as well as prevent cervical carcinoma.关键词：Zhiming Lingshuan;Xinxibao;chronic cervicitis;cervical carcinoma
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- 100
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- 15
发布时间：2024-09-23 - 摘要：目的:观察瘀痛舒丸治疗子宫内膜异位症腹腔镜术后的疗效。方法:将60例子宫内膜异位症患者随机分为对照组、治疗组各30例;对照组术后给予达那唑经期内开始服用,每次200 mg,2～3次/d,连续21 d,治疗组术后给予瘀痛舒丸经前期、经期连续口服7 d。两组均以3个月经周期为1个疗程,共2个疗程。比较两组患者综合疗效及血液流变学变化。结果:治疗组总有效率明显高于对照组,且血液流变性较对照组明显改善（P<0.05）。结论:瘀痛舒丸治疗子宫内膜异位症腹腔镜术后疗效肯定,值得临床推广应用。关键词：瘀痛舒丸;子宫内膜异位症;疗效观察
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发布时间：2024-09-23 - 摘要：Objective:To observe the therapeutic effect of Xingnaojing injection（XNJ） on secondary brain injury.Method:Sixty eight patients with severe craniocerebral injury were divided randomly into experimental group and control group,34 cases in each group.Conventional therapy was used in control group.In the base of control group,30 mL XNJ with 5% glucose injection 500 mL were used in experimental group,once a day,and a course of treatment was 10 days.Glasgow coma score（GCS）,hematoma,oedema and long-term quality of life were recorded before therapy and after therapy.Result:Compared with that before therapy,hematoma and oedema decreased,GCS and long-term quality of life score increased significantly in both two groups（P<0.01）.Compared with that in control group,oedema decreased,GCS and long-term quality of life score increased in experimental group,there was a significant difference（P<0.01）.Conclusion:XNJ can promote absorption of oedema,and improve consciousness of brain injury patients,efficacy of XNJ was better than that of conventional therapy.关键词：Xingnaojing injection;Chinese medicinal;secondary brain injury
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- 97
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- 13
发布时间：2024-09-23 - 摘要：目的:观察鹿瓜多肽注射液治疗创伤骨折58例的临床疗效。方法:116例患者随机分为观察组和对照组各58例。两组患者均采用切开复位钢板或髓内钉内固定,术后给予常规抗菌、抗感染对症治疗。观察组于术后第1天起以鹿瓜多肽注射液12 mL+生理盐水250 mL,gtt,1次/d,连续15 d。结果:观察组消肿止痛时间、骨痂生成时间、骨折愈合时间均明显短于对照组。结论:鹿瓜多肽注射液治疗创伤骨折临床疗效显著,可缩短肿胀时间、骨痂生成时间、骨折愈合时间。关键词：骨折;鹿瓜多肽注射液;治疗
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- 110
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发布时间：2024-09-23 - 摘要：目的:探讨天麻钩藤饮治疗社区高血压病的临床疗效。方法:该社区医疗中心的578例高血压患者随机分为治疗组、对照组各289例,治疗组口服中药天麻钩藤饮,对照组口服卡托普利（25 mg/次,3次/d）,疗程均为5周。观察记录治疗前后的临床症状,包括血压（BP）、胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）及治疗后的不良反应。结果:治疗后两组血压均明显下降（P<0.01）,治疗组症状改善明显优于对照组（P<0.01）,治疗组TC,TG明显降低,HDL-C明显升高,与对照组相比有明显差异。结论:天麻钩藤饮可明显降低血压,改善高血压病患者的临床症状,治疗组舒张压（DBP）下降比对照组更明显。关键词：天麻钩藤饮;卡托普利;社区疾病;高血压;血脂
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- 164
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发布时间：2024-09-23 - 摘要：目的:观察调经种玉汤联合枸橼酸氯米酚/人绒毛膜促性腺激素（CC/HCG）疗法治疗女性肾气虚型无排卵性不孕症的临床疗效。方法:将本组57例无排卵性不孕症患者随机分为两组,西药对照组28例,于月经出血第2天起服CC 50～100 mg,gd,5 d,当卵泡直径达1.8 mm时,im HCG 10 000 U/次,卵泡排出后im HCG 5 000U/次,隔日1次,共连续3次;中西药联合治疗组29例,在对照组治疗基础上加服调经种玉汤,随证加减)。均以3个月经周期为1个疗程。结果:两组卵泡发育及排卵率情况比较有显著性差异（P<0.01）,两组妊娠情况对比有显著性差异（P<0.01）。结论:调经种玉汤联合CC/HCG疗法治疗女性肾气虚型无排卵性不孕症疗效优于CC/HCG组。关键词：调经种玉汤;枸橼酸氯米芬;人绒毛膜促性腺激素;肾气虚型无排卵性不孕症
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发布时间：2024-09-23 - 摘要：目的:探讨中西医结合治疗龋病的临床疗效。方法:70例患者随机分为对照组和观察组各35例,两组患者均给予传统的去腐牙治疗,对照组给予氨硝酸银溶液治疗,观察组在对照组的基础上给予茵陈蒿与厚朴水煎服用进行治疗。观察两组患者的疗效、不良反应及复发情况。结果:对照组总有效率88.6%,观察组总有效率100.0%,两组差异显著（P<0.05）。观察组2例患者出现恶心,继续服药症状消失。对照组5例牙齿变黑。对照组与观察组的复发情况分别为第1个月8.6%和0,第2个月为11.4%和2.9%,第3个月为2.9%和0,组间比较P<0.05,差异有统计学意义。结论:中西医结合治疗龋病效果显著,可提高治愈率,降低复发率。关键词：中西医结合;龋病;临床观察;不良反应
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发布时间：2024-09-23 - 摘要：通过搜集和整理历代医家治疗骨痿的内服方剂用药,总结治疗该病的常用有效药物,对这些药物的性、味及归经规律进行研究,并探索相对高频次用药之间的配伍规律。利用《中华医典》为检索源,收集古代医籍中治疗骨痿的内服方剂,对其进行采集、整理和编排,查出药物的性、味及归经,总结用药规律。共搜集到治疗骨痿的方子155首,涉及药物180种,药物出现总频次1 497味次;用药频次排在前3位的是牛膝、熟地黄、肉苁蓉、菟丝子、茯苓;高频用药归经涉及除小肠经、三焦经之外的10条正经,出现频次共计2 414次,前两位的依次是肾经、肝经;所用药物涉及药性共5种,出现频次共计948次,前两位的依次是温、平;所用药物涉及药味共7种,出现频次共计1 481次,排在第1位的是甘。关键词：骨痿;计量学;聚类分析;用药规律
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发布时间：2024-09-23 - 摘要：Study on carbon nanotubes（CNTs） as drug carriers and their safety were introduced in this review.Recent evidence suggests that with the cross development of biology and material science,researchers realized that applications of new drug carriers are crucial to the research of drugs.Due to their unique structure,CNTs can be used as drug carriers to build unique drug delivery system.In some rare cases,functionalized CNTs with good solubility and low toxicity show good perspective in used as drug carriers.However,research of CNTs be used as drug carriers（especially traditional Chinese medicine） is rarely reported at home and abroad.Study on CNTs as carriers（adriamycin,epirubicin hydrochloride,dexamethasone,amphotericin B and Isoliquiritigenin,etc.） and its toxicity（tissue toxicity and cell toxicity） is summarized.Emerging studies suggest that application of traditional Chinese medicine as drug carriers is attractive.We hope to provide reference for the development of safe,controlled and efficacious new drug carriers and clinical treatment of related diseases.关键词：carbon nanotube;drug cartier;safety
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发布时间：2024-09-23 - 摘要：Objective:This article summarizes the mechanism of Chinese medicine treating anti-myocardial ischemia based on the collection and analysis of the associated literatures.Method:Search the databases including CNKI,PubMed,Elsevier etc and collect the literatures concerning about the effect of Chinese medicine on anti-myocardial ischemia in these five years,which were sorted by different mechanism.Result:The common mechanism of Chinese medicine treating anti-myocardial ischemia include the reduction of myocardial oxygen consumption,the clearing of free radicle,the inhibition of calcium overload and cell apoptosis,the adjustment of the ratio of TXA2/PGI2 and NO/ET-1,and the promotion of blood vessel neogenesis etc.Conclusion:The Chinese medicine can play the role of myocardial ischemia from different aspects,but the studies are still at an experimental stage,they even not reach the molecular genetic level,and more modern technology are needed in the future research.关键词：myocardial ischemia;Chinese medicine;mechanism of action;research progress
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- 2063
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发布时间：2024-09-23 - 摘要：This article deeply discusses the status of microdialysis technology for pharmacokinetic studies from sampling theory,methods,features,domestic application profiles and author experience combined with pharmacokinetic subject development.The microdialysis technology development trend and prospects of sampling site,sampling object,usage range,detection methods and sample concentration correction method,was explored according to author’s own practice.It was found that microdialysis sampling technique also has limitations,and thus the limitations and solutions was discussed mainly from sampling object of microdialysis technique in pharmacokinetic studies to provide a reference for researchers.关键词：microdialysis technology;pharmacokinetics study;development trend;limitations
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发布时间：2024-09-23 - 摘要：Objective:Depending on the patent application numbers of plant extracts,Chinese herbal drugs and Chinese patent medicines in China,Korea,Germany,Japan and USA from 2006 to 2010,the data were analyzed about their developing tends and to estimate consciousness of intellectual property rights.Method:Patent numbers of plant extracts,Chinese herbal drugs and Chinese patent medicines in China,Korea,Germany,Japan and USA from 2006 to 2010 were obtained from official webs in China,Korea,Germany,Japan and USA.Result:The results showed that the numbers of plant extracts,Chinese herbal drugs and Chinese patent medicines were going down in China,Korea,Germany,Japan and USA,while for the patent application number of plant extracts,Korea was first most and China was second;The patent application of Chinese herbal drugs was not so much,in which China was first most and Korea second.For the patent application number of Chinese patent medicines,China is the much more than the other countries.Conclusion:The R&D for Chinese traditional medicines has been shown to slip down.For the total patent application number of Chinese traditional medicines,China was much higher than the other countries,whereas the other countries special Korea were also developed.Oppositely protection of intellectual property rights of Chinese traditional medicines should be strengthened further.More R&D should be invested in Chinese traditional medicines.关键词：medical intellectual property;Chinese traditional medicine;plant extracts;Chinese herbal drugs;Chinese patent medicines
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- 1398
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发布时间：2024-09-23 - 摘要：温病卫分证是由于病邪、体质和兼夹的不同,其症状复杂,证候繁多,临床辨证时有一定难度,但也有规律可循。笔者在长期的教、医、研实践中,对卫分证的辨证规律有初步的肤浅认识,即:在确定诊断为温病的前提下,首先通过阶段分析法、综合分析法、排除分析法和寒温详辨法,确立邪在卫分;次审卫分具体病因,辨明风热、燥热、暑湿、湿热等的不同;再进一步详察卫分的病位重心,如肺、脑等;最后还要细审证候兼夹,如虚、瘀、食滞等。临证时有正确的辨证思路,方能达到准确辨证的目的。关键词：温病;卫分证;辨证规律
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- 280
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发布时间：2024-09-23 - 摘要：池晓玲教授从事中医肝病临床研究近30载,临床经验丰富,擅长运用中医天人相应观对临床常见肝病进行辨证与辨病治疗。池教授通过对258例慢性乙型肝炎病例进行观察,发现患者肝功能的变化呈现季节性的规律,其主要原因大体有三,即五脏应四时阴阳,患者存在着贯穿疾病始终的"脾虚"内因以及内外湿热胶结等因素,并且指出对肝功能呈季节性变化的慢性乙型肝炎患者的治疗应当根据不同的季节、不同的症状表现进行辨治。关键词：慢性乙型肝炎;肝功能;季节性
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发布时间：2024-09-23 - 摘要：<正>笔者于1998年2月至2009年2月采用清毒降酶汤配合苦参碱（斯巴特康）注射液（广州明兴制药厂生产）治疗慢性乙型肝炎350例,疗效满意。1临床资料350例乙型肝炎是根据1990年5月全国病毒性肝炎会议的标准进行诊断,均为我院门诊及住院患者,乙肝HBsAg,HBeAg或HBV-DNA阳性持续6个月以上,血清丙氨酸氨基转移酶（ALT）>166 U.L-1（正常<40 U.L-1）,血清门冬氨
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发布时间：2024-09-23 - 摘要：<正>哮喘是由嗜酸性粒细胞、肥大细胞和T淋巴细胞等多种炎性细胞参与的气道慢性炎症。临床上表现为反复发作性的喘息、呼气性呼吸困难、胸闷或咳嗽等症状。传统医学中有哮、肺胀、喘吼等多种病名,是临床常见病、多发病。该病难以根治且易反复发作。目前,西医治疗主要是以解痉抗炎为主,远期疗效不理想,而且毒副反应较大。传统医学从整
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发布时间：2024-09-23 - 摘要：<正>毛细支气管炎是婴幼儿时期较常见的下呼吸道感染性疾病,多见于6个月以内婴儿,临床表现主要为喘息、呼吸急促、三凹征、喘鸣、严重者可发生心力衰竭、呼吸衰竭、中毒性脑病等严重并发症而危及生命。本研究使用细辛脑注射液联合硫酸镁辅助治疗小儿毛细支气管炎,取得满意疗效。1资料与方法
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发布时间：2024-09-23 - 摘要：<正>皮肤压疮是因为患者长期卧床,局部受压出现血液循环障碍,局部组织缺血、缺氧造成组织溃疡坏死,一旦发生比较难治愈。如继发感染,则加重病情引起患者出现败血症,甚至危及生命。常用的治疗方法是应用抗生素换药,治疗时间比较长,给患者带来很大的痛苦,效果不满意。我院应用京万红软膏治疗肺癌长期卧床压疮患者,疗效满意。
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发布时间：2024-09-23 - 摘要：<正>月经过少是指月经周期正常,月经量明显减少,或行经时间不足2 d,甚或点滴即净者,有时也与周期异常并见,如先期伴量少,后期伴量少,后者往往为闭经的先兆,如不及时治疗可发展成为闭经、不孕、卵巢早衰等。故及时治疗本病对维持女性正常的生理功能、保持生育能力、延缓衰老有着积极的临床意义。
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发布时间：2024-09-23 - 摘要：<正>长生果胶囊是国际合作项目"筛选治疗2型糖尿病中药复方有效单体及有效单体最佳配伍的实验研究（No.2008DFA31050）"科研方,由桑椹（又名长生果）、栀子、沙棘、山药等中药组成,具有益气养阴、清热泻火、活血化瘀的功效,可用于本虚标热、病久兼瘀之消渴病。据《新修本草》记载,桑椹"单食,主消渴";《本草新编》记载桑椹"专黑髭须,尤能止渴润燥,添精益脑"。《药性论》记载栀子"除热及消渴"。《日华子本草》记载山药"助五脏,强筋骨,长志安神,
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发布时间：2024-09-23 - 摘要：<正>专家介绍刘亚明教授,博士生导师,山西中医学院科技产业处处长,山西省中药现代化科学技术平台负责人。兼任中华中医药学会理事,中华中医药学会国际合作与交流分会副主任兼秘书,中国康复医学会常务理事,中国管理科学院特聘研究员,国家科技部"十二五"科技支持计划项目华北地区首席专家,山西省委联系的高级专家,山西省人民政府经济结构调整医药组专家,山西省经济结构调整办公室特聘专家,中华中医药管理杂志编委,中国实验方剂学杂志编委,中医药学刊杂志编委,山西中医学
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发布时间：2024-09-23 - 摘要：<正>专家介绍李孝栋（1969.11—）,博士,福建中医药大学药学院副教授,硕士生导师,美国访问学者,福建省药学会药剂专业委员会委员,中国实验方剂学杂志编委。1988至2004年先后获得沈阳药科大学学士、硕士、博士学位,导师潘卫三、吴立军教授。博士论文《中药附子理中方新剂型的制备及其药物动力学的研究》。现从事教学和科研工作,先后主讲《中药药剂学》、《药剂学》、《生物药剂学与药物动力学》、《药学英
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发布时间：2024-09-23
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