最新刊期
卷 19 ， 期 14 ， 2013
- 摘要：Objective: To investigate degradation kinetics of main ingredients (salvianolic acid B and rosmarinic acid) of salvianolate lyophilized injection under different pH and temperatures. Method: HPLC was adopted to determine the content of salvianolic acid B and rosmarinic acid at different pH values and temperatures,degradation kinetic parameters of these two ingredients were evaluated by chemical kinetics. Result: Reaction of salvianolic acid B and rosmarinic acid at different pH values and temperatures were accord with the first-order kinetics,degradation rate accelerated by increasing of pH value and temperature.According to arrhenius equation,activation energy (Ea) of salvianolic acid B in aqueous solution and salvianolate lyophilized injection were 48.54,95.19 kJ·mol-1,respectively.Ea of rosmarinic acid in aqueous solution and salvianolate lyophilized injection were 49.83,83.56 kJ·mol-1,respectively. Conclusion: Salvianolic acid B and rosmarinic acid were sensitive to strong alkaline and high temperature conditions,but salvianolate lyophilized injection could obviously improve their stability.关键词：salvianolic acid B;rosmarinic acid;salvianolate lyophilized injection;degradation kinetics
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发布时间：2024-01-04 - 摘要：Objective: To prepare affinity medium with four effective ingredients of ginsenoside Rg1,ginsenoside Rb1,ophiopogonin DT-13 and schisandrin from Shengmai powder as ligands. Method: Affinity medium was prepared by coupling with four effective ingredients and epoxy-activated-sepharoseTM6B(EAS 6B).A HPLC-DAD method for simultaneous separation and determination of four ingredients was established,with coupling rate as index,effect of feeding rate,reaction time and temperature on coupling reaction was investigated by orthogonal test,then optimized preparation technology. Result: Optimum preparation technology was as following:reaction time 24 h,reaction temperature 25℃,added 10 times the amount of active ingredients ligands.Four ingredients could coupling with EAS 6B,coupling rate with four ingredients and affinity medium was ginsenoside Rg1 of 27.40%,ginsenoside Rb1 of 22.30%,ophiopogonin DT-13 of 50%,schizandrin of 24.50%. Conclusion: We successfully prepared affinity medium with four effective compounds deprived from Shengmai powder as ligands.It provided technical means to further identify target of this compound and some references for preparing affinity medium of other multiple natural compounds with similar active groups.关键词：affinity medium;active ingredients prescription from Shengmai powder;epoxy-activated-sepharoseTM6B;orthogonal design;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize parameters in processing Fructus Gardeniae Praeparatus and investigate content change of crocin-I and crocin-II in processing. Method: With the mass fraction of water extract,ethanol extract and geniposide as comprehensive evaluation index,orthogonal test was used to investigate effect of frying temperature and frying time on processing technology of Fructus Gardeniae Praeparatus.HPLC was adopted to determine the content of crocin-I and crocin-II in processing. Result: Optimum processing conditions were as follows:fried 30 min at 180℃.The content of crocin-I and crocin-II gradually reduced in processing,they would decompose completely when temperature was too high in processing. Conclusion: Properties of Fructus Gardeniae Praeparatus complied with relevant regulations,this study could provide experimental basis for quality control of Fructus Gardeniae Praeparatus.关键词：Fructus Gardeniae Praeparatus;orthogonal test;content determination;crocin
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发布时间：2024-01-04 - 摘要：Objective: To establish HPLC fingerprint detection for alkaloids from extract of Sophora flavescens in Yuchangning capsules. Method: Amino Kromasil NH2 column (4.6 mm×250 mm,5 μm) was adopted,gradient eluted with mobile phase of acetonitrile-ethanol (8:1)-3% phosphoric acid water,flow rate was 0.8 min·mL-1,detection wavelength was 220 nm.10 batches of extract of S. flavescens were detected by HPLC fingerprint,then evaluated with 2004 edition of ‘chromatographic fingerprint evaluation system of Chinese medicine’. Result: By taking Kromasil NH2 column,peaks separation of extract of S. flavescens was better,identified 15 common peaks and 4 of them were assigned. Similarity of these 10 batches extract was more than 0.9, correlation between fingerprints of extract and raw materials existed. Conclusion: This method had good precision,repeatability and stability,it could provide a detection method for quality control of extract of S. flavescens.关键词：Sophora flavescens;extract;alkaloids;fingerprint
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发布时间：2024-01-04 - 摘要：Objective: To observe thermal stability of β-cyclodextrin inclusion complex of volatile oil from Xiaoyu Anshen capsules. Method: The content of patchouli alcohol at different temperatures from β-cyclodextrin inclusion complex of volatile oil was determined by GC-MS.Chromatographic conditions were as follows:HP-5 capillary column(0.25 mm×30 m,0.25 μm),took selective ion mode (SIM),voltage of detector set at 1.5 kV. Result: The content of patchouli alcohol was no significant change after inclusion complex of volatile oil heating 4 h at different temperatures (40,60,80℃). Conclusion: Thermal stability of β-cyclodextrin inclusion complex of volatile oil from Xiaoyu Anshen capsules was good,it could guide molding process of this preparation.关键词：Xiaoyu Anshen capsules;patchouli alcohol;β-cyclodextrin inclusion complex;thermal stability
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Zhizhu granules. Method: Based on single factor tests,with yield of synephrine as index,effect of water addition,extraction time and times on extraction technology of Zhizhu granules was investigated by Box-Behnken response surface methodology. Result: Optimum extraction technology was as following:took prescription amount of raw materials,reflux extracted 3 times with 490 mL water,115 min each time;Average yield of synephrine was up to 90.57%. Conclusion: Box-Behnken design was suitable for optimizing extraction technology of Zhizhu granules,the observed value approximated to the predicted value of established mathematical model.关键词：Zhizhu granules;synephrine;Box-Behnken design;response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To explore in vivo transformation form of aqueous extract W-A-80 from Trionycis Carapax through investigating its digestion in simulated gastric fluid(SGF) and simulated intestinal fluid(SIF). Method: Aqueous extract W-A-80 from Trionycis Carapax was prepared by water extraction and alcohol precipitation method,it was digested at 37℃ in SGF and SIF.With yield of oligopeotides as index,effect of different enzyme-substrate ratios and reaction time of pepsin and trypsin on yield change of oligopeptides(interception relative molecular mass<3 500) was investigated by enzyme-linked immunosorbent detector-biuret reaction;Equilibrium dialysis was adopted to determined protein binding rate of prepared oligopeptides with liver tissue of mice in vitro. Result: Optimum enzymatic hydrolysis conditions were determined that digested 3 h with pepsin enzyme substrate ratio of 1:10 at 37℃ in SGF and 11 h with trypsin enzyme substrate ratio of 1:100 at 37℃ in SIF;Protein binding rates of different concentrations of oligopeptides with liver tissue in mice were 31.63%,19.93%,9.65% and 48.75%,47.43%,22.72%,respectively,both significantly more than that of blank control group which were 7.00%,5.31%,4.60%. Conclusion: A large amount of oligopeptides generated when aqueous extract W-A-80 was digested in simulated gastrointestinal fluid,and their activity were significantly stronger than undisposed,so we speculated oligopeptides may be materials basis of Trionycis Carapax for anti-liver fibrosis.关键词：Trionycis Carapax;simulated gastric fluid;simulated intestinal fluid;oligopeptides;liver tissue protein binding rate
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发布时间：2024-01-04 - 摘要：Objective: To optimize supercritical CO2 fluid extraction process parameters of volatile oil from Angelica sinensis. Method: With composite score of the content of ligustilide and yield of volatile oil as index,the content of ligustilide was determined by HPLC,L9(34) orthogonal test was used to investigate effect of extraction temperature,extraction pressure,extraction time on extraction efficiency. Result: Optimum process parameters were as follows:extraction pressure 30 MPa,extraction temperature 50℃,extraction time 2 h,flow rate of CO2 25 L·h-1,pressure of separation kettle I 8 MPa with temperature at 50℃,separation pot II pressure of the system tail pressure with temperature at 35℃. Conclusion: This optimized process was stable and feasible,which could be adopted to extract volatile oil from A. sinensis with high yield and good quality.关键词：Angelica sinensis;supercritical CO2 fluid extraction;ligustilide
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发布时间：2024-01-04 -
Optimization of Ultrasonic Extraction Technology and Content Determination of Total Amino Acids from
摘要：Objective: To optimize extraction technology of total amino acids from Dendrobium officinale and establish its content determination method. Method: The content of total amino acids was determined by UV with aspartic acid as reference;Effects of the concentration of extraction solvent,solid-liquid ratio,extraction temperature,extraction time and times on yield of total amino acids were investigated by single factor tests,basis on these,extraction technology was optimized by orthogonal test. Result: Optimum ultrasonic extraction conditions were as follow:extracted 1 time at 60℃ with 20 times the amount of 0.1 mol·L-1 HCl for 30 min. Conclusion: This optimized technology was stable and feasible,established method of content determination was convenient and accurate with good repeatability.关键词：Dendrobium officinale;total amino acids;ultraviolet spectrophotometry;content determination;extraction technology- 2
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determining the content of sodium in extract of Salvia miltiorrhiza. Method: With nitric acid as digestion system,extract of S. miltiorrhiza was digested by microwave,the content of sodium belonging to this extract was determined by inductively coupled plasma mass spectrometry(ICP-MS).In order to correction response signal changes and eliminate influence of matrix effect and instrument drift,chromium was selected as an internal standard solution. Result: Linear range of sodium was 0.00-10.00 mg·L-1(r=0.999 9),with poplar leaves as work standards,average recovery was determined as 90.4% (RSD 3.8%,n=6). Conclusion: This method had good stability,reproducibility and precision,it could be used to determining the content of sodium in extract of S. miltiorrhiza.关键词：extract of Salvia miltiorrhiza;sodium;content;ICP-MS
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发布时间：2024-01-04 - 摘要：Objective: To isolate and identify chelerythrine and sanguinarine monomer. Method: Chelerythrine and sanguinarine were isolated and purified by column chromatography technology,and identified by HPLC. Result: Single component could be obtained by column chromatography method,chelerythrine and the sanguinarine monomer were identified by HPLC with purity of 98.6%,95.7%,respectively. Conclusion: Column chromatography technology could obtain high purity chelerythrine and sanguinarine monomer from Macleaya cordata.关键词：column chromatography;chelerythrine;sanguinarine;Macleaya cordata;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To optimize prescription of Keyou nanoemulsion. Method: With composite score of appearance and particle size as dependent variable,ratio of mixed surfactant-isopropyl(IPM),tween 80-ethanol and water-surfactant as independent variables,blank prescription of Keyou nanoemulsion was optimized by central composite design-response surface methodology,and then added drug to prepared blank Keyou nanoemulsion. Result: Optimal prescription was as following:mixed surfactant-IPM (8.090:1),tween 80-ethanol (3.082:1),water-surfactant (8.606 1:1).Average particle size of prepared Keyou nanoemulsion was 27.46 nm,Zeta potential value was -0.522 4 mV. Conclusion: This optimized process was stable and feasible for preparation of Keyou nanoemulsion.关键词：Keyou nanoemulsion;central composite design response surface methodology;particle size
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发布时间：2024-01-04 - 摘要：Objective: To optimize purification technology of polysaccharides from Isatis indigotica by freezing and thawing-enzymolysis-membrane separation composite technology. Method: With the content of polysaccharides from I. indigotica and remaining protein as indexes,based on single factor tests,orthogonal test was adopted to investigate effect of hydrolysis temperature,reaction time and enzyme dosage on purification technology. Result: Optimal purification process was as following:repeated freezing and thawing three times of water extract liquid of polysaccharides from I. indigotica residue,filtered,added protease 2 with dosage of 35 000 U·g-1,enzymatic temperature for 35℃,hydrolysis time of 3 h,then ultrafiltrated with hollow fiber components of interception molecular mass 30 kD. Conclusion: This optimized technology was stable and feasible,freezing and thawing-enzymolysis-membrane separation composite technology could be used to purify polysaccharides from I. indigotica.关键词：Isatis indigotica;polysaccharides;purification technology
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发布时间：2024-01-04 - 摘要：Objective: To investigate in vitro transdermal absorption properties of compound safflower effervescent granules. Method: In vitro transdermal absorption experiment was performed through diffusion cell method,the content of total alkaloids from Aconitum carmichaeli was determined by UV in order to choose suitable receiving liquid and receiving liquid temperature,then transdermal effect of compound safflower effervescent granules was investigated. Result: Physiological saline was selected as receiving liquid with temperature at 32℃,transdermal absorption effect of compound safflower effervescent granules was better than extraction liquid and common granules. Conclusion: Compound safflower effervescent granules had good transdermal absorption effect.关键词：topical preparations;transdermal absorption;Carthamus tinctorius;effervescent granules;total alkaloids from Aconitum carmichaeli
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of Cynanchum paniculatum in Zhenggu mixtures. Method: With the content of paeonol as index,which was determined by HPLC,effect of grinding degree,ethanol concentration and extraction time on extraction technology was investigated by orthogonal test. Result: Optimum extraction technology conditions were as follows:grinding degree of raw material 20 mesh,maceration extracted 60 h with 70% ethanol,yield of paeonol 5.34 mg·g-1. Conclusion: Optimized technology was stable and feasible,this study could provide a reference for industrial production of Zhenggu mixtures.关键词：Zhenggu mixtures;Cynanchum paniculatum;paeonol
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of total flavonoids from diaphragm of Juglans regia. Method: With luteoloside as quantitative index,based on single factor tests,effect of ethanol concentration,solid-liquid ratio and mesh of raw material on yield of total flavonoids was investigated by orthogonal test. Result: Optimal extraction technology was as following:grinding degree of raw material 60 mesh,ultrasonic extracted with 15 times the amount of 60% ethanol,yield of total flavonoids was up to 2.985 mg·g-1. Conclusion: This optimized technology of total flavonoids was reasonable,reproducible and stable,it could provide basis for resources utilization of J. regia.关键词：diaphragm of Juglans regia;flavonoids;orthogonal test;ultrasonic extraction;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To purify polypeptide from Eupolyphaga sinensis and investigate its thrombolytic activity. Method: With thrombolytic activity as index,polypeptide was obtained by bionic enzymatic,which was purified by Sephadex G-25,SP Sephadex C-25 and RP-HPLC C18 semi-preparation column.Thrombolytic activity,peptide content and relative molecular weight of target component were determined. Result: Thrombolytic activity of target polypeptide component was 814 430 U·mg-1,peptide content was 95.7% and relative molecular weight was in the range of 3 211-3 547. Conclusion: Polypeptide from E. sinensis could be prepared by bionic enzymatic,and a component of polypeptide with strong thrombolytic activity could be obtained through separation and purification.关键词：Eupolyphaga sinensis;Polypeptide;purification;thrombolytic activity;bionic enzymatic
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发布时间：2024-01-04 - 摘要：Objective: To investigate rapid dissolution mechanism of α-asarone by liquisolid compacts. Method: α-Asarone liquisolid tablets were prepared with solid-liquid ratio of 1:4,tween-80 as liquid excipient,microcrystalline cellulose PH-101 as carrier material,aerosil®200 as coating material.Dissolution of these liquisolid tablets and common tablets was compared,rapid release mechanism of these liquisolid tablets was investigated by measuring X-ray diffraction (XRD) and disintegration time. Result: Dissolution of α-asarone in liquisolid tablets was more than 80% in 5 min.XRD showed that there was no characteristic peaks of α-asarone in liquisolid tablets. Conclusion: Liquisolid compacts could improve drugs wetting properties and increase effective surface area of drugs dissolution,then allowing rapid dissolution of poorly soluble drugs.关键词：α-asarone;tween-80;liquisolid tablets;dissolution;X-ray diffraction
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发布时间：2024-01-04 - 摘要：Objective: To optimize extraction technology of dehydroevodiamine(DHED) from Evodia rutaecarpa by response surface methodology. Method: With yield of DHED as index,single factor tests and response surface methodology were adopted to investigate effect of crushed mesh of drugs,solid-liquid ratio,the concentration of alcohol and extraction time on yield of DHED. Result: Optimum extraction technology of DHED was as following:crushed degree of E. rutaecarpa 90 mesh,extracted 0.82 h with 18.73 times the amount of 65.22% ethanol,and yield of DHED was up to 6.97 mg·g-1. Conclusion: It was feasible to optimize extraction technology of DHED from E. rutaecarpa by response surface methodology,it could provide basis for quality control of E. rutaecarpa.关键词：Evodia rutaecarpa;dehydroevodiamine;response surface methodology
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发布时间：2024-01-04 - 摘要：Objective: To optimize separation and purification technology of syringin in Acanthopanax obouatus with macroporous absorption resin. Method: With the content of syringin as index,which was determined by HPLC,model of macroporous absorption resin was screened by static adsorption-elution test,purification conditions were investigated by single factor tests. Result: XDA-1 macroporous adsorption resin model was adopted,its purification technology was as following:the amount of sample 3.5 mg syringin with 1 g XDA-1 macroporous absorption resin,adsorption velocity 1 BV·h-1,eluted with 9 BV 40% ethanol at 2 BV·h-1.Purity of syringin was up to 28.14% from 5.47%. Conclusion: XDA-1 macroporous resin could be used to purify extract of A. obouatus,yield and purity of syringin obtained satisfactory results.关键词：macroporous absorption resin;Acanthopanax obouatus;syringin;separation;purification
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发布时间：2024-01-04 - 摘要：Objective: To prepare sulfated polysaccharides from Polyporus umbellatus and investigate its anti-oxidation activity. Method: Polysaccharides was extracted and isolated by alcohol precipitation,macroporous resin,polyamide resin,deproteinization from P. umbellatus. Polysaccharides was sulfated by sulfuric acid-pyridine method,sulfated polysaccharides was depurated by Sephadex G-75 and dialysis,and in vitro scavenging effect of sulfated polysaccharides for hydroxyl free radical was observed. Result: Before and after sulfated,polysaccharides from P. umbellatus showed good antioxidation in scavenging hydroxyl free radical from Fe2+-H2O2 system,scavenging capacity of sulfated polysaccharides for hydroxyl free radical enhanced,and showed a clear dose-effect relationship. Conclusion: This method of preparing sulfated polysaccharides was accurate and reliable,it could provide references for further development and utilization of polysaccharides from P. umbellatus.关键词：sulfated polysaccharide from Polyporus umbellatus;preparation;activity assay
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发布时间：2024-01-04 - 摘要：Objective: To optimize formulation technology of total flavonoids from Bidens bipinnata sustained release tablets. Method: With hydroxypropyl methyl cellulose K4M (HPMC-K4M) as skeleton materials,starch and lactose as fillers,cumulative release of total flavonoids at three time points as comprehensive evaluation index,based on single factor tests,orthogonal test was used to investigate effect of the amount of HPMC-K4M,starch-lactose,tablets hardness and the amount of anti-adhesion on formulation technology. Result: Optimum prescription of total flavonoids from B. bipinnata was as following:the amount of HPMC-K4M 50%, starch-lactose (2:1),tablets hardness 5 kg·mm-2,the amount of anti-adhesion(gum acacia) 2%. Conclusion: This optimized technology was stable and feasible,these prepared sustained-release tablets had good granulation,tabletting and sustained release,which were in line with relevant regulations.关键词：Bidens bipinnata;total flavonoids;sustained release tablets;single factor test;orthogonal test
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发布时间：2024-01-04 - 摘要：Objective: To develop based formulation of Cervus elaphus amino acid oral liquid. Method: Selected two C. elaphus powder from the same origin,divided into the biomimetic group and the ultrasound group according to different extraction methods.The content of amino acid of different groups was compared,and SIMCA-P statistical software was adopted to do principal component analysis. Result: There was a significant difference between samples from two groups, especially methionine, proline, arginine, threonine, isoleucine, phenylalanine and valine. Formulation of core amino acids group was as following:methionine 0.96%,proline 11.50%,arginine 8.29%,threonine 2.64%,isoleucine 1.71%,phenylalanine 2.62% and valine 3.46%;Formulation of based amino acids group was aspartic acid 7.39%,serine 4.07%,glutamic acid 13.31%,glycine 21.72%,alanine 10.30%,leucine 4.64%,tyrosine 0.97%,lysine 5.07%,histidine 1.36%. Conclusion: There are some differences in amino acid proportion between the natural state and the digestion state.With amino acids proportion as basis,this developed C. elaphus amino acid oral liquid could get better tonic effect of C. elaphus.关键词：Cervus elaphus;amino acid oral liquid;formula;bionic
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发布时间：2024-01-04 - 摘要：Objective: To establish quality standards of Shengxue Xiaoban tablets. Method: TLC was adopted to identify Indigo Naturalis,Forsythiae Fructus and Moutan Cortex.Indirubin was as quantitative indicroe,whose content was determined by RP-HPLC,chromatography conditions were as follows: Discovery C18 column(4.6 mm×200 mm,5 μm),mobile phase of methanol-0.1 mol·L-1 ammonium acetate solution-acetic acid (80:20:1),detection wavelength 290 nm. Result: TLC identification showed that negative controls were no interference;Linear range of indirubin was 0.010 95-0.394 2 μg(r=0.999 8),average recovery was 97.4% with RSD of 0.7%. Conclusion: This established method was simple and accurate which could be used to quality control of Shengxue Xiaoban tablets.关键词：HPLC;Indigo Naturalis;Forsythiae Fructus;Moutan Cortex;Shengxue Xiaoban tablets;indirubin;content determination
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发布时间：2024-01-04 - 摘要：Objective: To study the condition of heat reflux extraction of arecoline from Areca inflorescence. Method: With yield of arecoline as index, effect of solid-liquid ratio, extraction time and extraction times on the yield of arecoline was investigated by L9 (34) orthogonal test to obtain the best extraction conditions. Result: The optimum condition of heat reflux extraction of arecoline from A. inflorescence was as follows:ratio of liquid to material 80:1, extraction time 25 min, 3 times for extraction. The yield of arecoline was (4.31±0.047) mg·g-1. Conclusion: This optimized technology is simple, efficient and suitable for extraction of arecoline from A. inflorescence. It has significant differences compared with old heat reflux extraction technology which yield of arecoline is (3.38±0.03) mg·g-1.关键词：Areca inflorescence;arecoline;heat reflux extraction;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To study the phenylpropanoids chemical constituents from the root of Dendropanax chevalieri, and evaluate the biological activity of the nine phenylpropanoids including the five phenylpropanoids isolated previously, and to provide preliminary pharmacological basis for the clinical application of the root of D. chevalieri in medicine of She nationality. Method: The constituents were isolated from n-butanol extract of D. chevalieri 95% ethanol extract by all kinds of column chromatography. Their structures were elucidated by analysis of physical chemical properties and spectral data. By using phorbol myristate acetate (PMA) as the stimulant, luminol-dependent chemiluminescence was used to compare the effects of the nine phenylpropanoids compounds on the respiratory burst of rat neutrophils. Result: Four phenylpropanoid compounds, sinapaldehye glucoside (1), syrigin (2), coniferaldehyde 4-O-β-D-glucopyranoside (3) and icariside E5 (4), were isolated and identified from n-butanol extract. As results of pharmacological studies, six phenylpropanoid compounds revealed different inhibitory activity on the respiratory burst of rat neutrophils. Their activity were ranked in the decreasing order of scopoletin>(+)-pinoresinol>(+)-syringaresinol coniferaldehyde>β-hydroxypropiovanillon>VC>icariside E5, while sinapaldehye glucoside, syrigin and coniferaldehyde 4-O-β-D-glucopyranoside had no inhibitory activity. Conclusion: Compound 3 is isolated from this genus for the first time. Phenylpropanoid compounds isolated from the root of D. chevalieri has a strong anti-PMN respiratory burst activity and hydroxyl group might be a pharmacophore. This is the fist report of the pharmacological evidence for the clinical application of the root of D. chevalieri in medicine of She nationality.关键词：Dendropanax chevalieri;phenylpropanoid;PMN;respiratory burst;chemiluminescence
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发布时间：2024-01-04 - 摘要：Objective: To analyze and compare the chemical constituents of the volatile oil from trunk, branch and root bark of Melia azedarach. Method: The volatile oil was extracted by steam distillation and analyzed by GC-MS. The relative content of each constituent was calculated by normalization. Result: 47, 62 and 56 components were separated and identified respectively from trunk, branch and root bark of M. azedarach The main composition of volatile oil of trunk, branch and root bark was linoleic acid (38.574%, 27.178%,respectively), root bark was α-copaene (18.057%). Conclusion: The volatile components in different parts of meliae Cortex are also significantly different. Meliae Cortex can be extracted and utilized on the basis of different parts.关键词：Melia azedarach;volatile oil;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To develop a method for determining the content of paeoniflorin in containing serum of rats. Method: The content of paeoniflorin from Xuefu Zhuyu capsules was determined by HPLC,but it was determined by HPLC-MS-MS in serum. Result: The content of paeoniflorin from Xuefu Zhuyu capsules was 1.68 mg/particle,which was in line with requirements in the 2010 edition of "Chinese Parmacopoeia";Linear relationship of paeoniflorin in serum was 4.57-585 μg·L-1 (r=0.998 3).Recovery of this method was 101.7%-115.5%. Conclusion: Determination the content of paeoniflorin in containing serum of rats was stable and reproducible by HPLC-MS-MS,it could be as a quality control method for Xuefu Zhuyu capsules in serum of rats.关键词：Xuefu Zhuyu capsules;containing serum;paeoniflorin;HPLC-MS-MS;determination
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发布时间：2024-01-04 - 摘要：Objective: To study the function of retention index (RI) on the qualitative results based on the MS qualitative outcome of volatile oil from Chinese material medica (CMM) as Citri Reticulatae Pericarpium Viride(CRPV). Method: The total ion chromatograms from essential oil of CRPV and referred materials as series n-alkanes were gotten by the GC-MS. The RIs of constituents in volatile oil were calculated by a related formula. The components were recognized by MS and RIs. Result: Some compounds have essentially identical mass spectra, while they can be discriminated by RIs. Conclusion: RI plays an important role in the identification of constituents in essential oil from CMM.关键词：retention index (RI);Citri Reticulatae Pericarpium Viride (CRPV);volatile oil;qualitative analysis;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: Explore the Rhizoma Musae treating Xiaoke emphasis on the fresh juicer liquid instead of dry goods decoction reasons to provide a reference for the rational utilization of Rhizoma Musae. Method: According to the clinical applications of Rhizoma Musae, petroleum ether was used to extract fresh juicer liquid and dry goods decoction, and the chemical compositions were separated, analyzed and identified by gas chromatography-mass spectrometry;peak area normalization method was applied to calculate the relative percentage of the respective components. Result: There were 54 chemical compositions identified in the petroleum ether extract of fresh juicer liquid. The relative content of palmitic acid was 3.13%. However, only 4 chemical compositions were identified in the petroleum ether extract of dry goods decoction, the palmitic acid relative content was up to 25.60%. Conclusion: The study results show that the ancient and modern literatures recorded the usage of Rhizoma Musae juicer liquid to cure ‘Xiaoke’ rather than dry goods decoction have a certain scientific reason.关键词：Rhizoma Musae;juicer liquid;decoction;petroleum ether extract;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To optimize the method for the ester-type alkaloids determination in aconites medicinal material. Method: We compared the preparation methods of sample and reference solutions according to procedure in the assay under Aconiti lateralis radix praeparata and Radix Aconiti Praeparata in ChP. VolⅠ2010 Edition. Result: When the samples were extracteded with HCl solution (0.05 mol·L-1) by sonication,the contents of six ester-type alkaloids were higher than which extracted with method of ChP. VolⅠ(2010 Edition). When the reference substances were dissolved in 0.05%HCl-methanol,the six ester-type alkaloids were stable. Conclusion: The method is accurate,credible and provide reference for improving the method for the ester-type alkaloids determination of aconites medicinal material in ChP.关键词：Aconiti Lateralis Radix Praeparata;Radix Aconiti Praeparata;sample preparation;ester-type alkaloids;determination
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for content determination of loureirin A, B in Sanjie Zhentong capsules. Method: Extracted loureirin A, B with ultrasonic method in the use of 75% methanol, Kromasil C18 column (4.6 mm×250 mm, 5 μm) was used with a step gradient of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL·min-1. The sample amount was 10 μL and the detection wavelength was set at 278 nm. The column temperature was maintained at 30℃. Result: Loureirin A and B had a good linear relationship during 526.20-4.11 ng (r=0.999 9) and 506.09-3.95 ng (r=0.999 9) respectively. The average recoveries were 101.84% with RSD 0.77%(n=6), 100.54% with RSD 1.46%(n=6). The method also had good reproducibility and stability. Conclusion: The sample preparation is reasonable in the quantitative method, and the accurate results can be used for content determination of loureirin A and B of Sanjie Zhentong Capsules.关键词：Sanjie Zhentong capsules;loureirin A;loureirin B;content determination;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop the quality standard of Yangxin Dawayi Mixike Migao. Method: The TLC method was used for the qualitative identification of Stigma Croci and Cortex Cinnamomi in the formula. The content of crocin-I in Yangxin Dawayi Mixike Migao was determined simultaneously by RP-HPLC method with a Luna C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was methanol-water (45:55);the flow rate was 1.0 mL·min-1. The detection wavelength was set at 440 nm, and the column temperature was set at 35℃. Result: The identification study showed strong specificity. The linear relationship was good. The linear response ranged from 6.04 μg to 45.3 μg for crocin-I and the average recovery was 98.7% with RSD 1.51%. Conclusion: The methods were simple, accurate and with a good reproducibility, and can be used for the quality control of the drug.关键词：Yangxin Dawayi Mixike Migao;quality standards;TLC;Stigma Croci;Cortex Cinnamomi;RP-HPLC;crocin-I
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发布时间：2024-01-04 - 摘要：Objective: To analyze the chemical constituents of volatile oil from the seeds and shells of Amomum taso-Ko, and provide a basis for its development and application. Method: The steam distillation was used to the extraction of volatile oils and then calculated its content. The constituents of these volatile oils were separated and identified by GC-MS. Result: 35, 48, 42 compounds of three volatile oils of Amomum tsao-ko seeds from different origins were identified respectively, which account for 94.8%, 95.6%, 93.7% of their total peak area respectively. 55, 70, 68 compounds of the volatile oil samples from Amomum tsao-ko shells were identified, which account for 94.5%, 91.5%, 91.4% of their total oils. There were 29 common constituents in the volatile oil from the seeds and shells of Amomum taso-ko, which were 1,8-cineole, α-pinene, β-pinene, α-terpineol, nerolidol, and so on, which account for 95.4%-94.1% and 71.0%-77.6% of the total oils from the seeds and shells respectively. There were 18 special constituents in the volatile oils from the shells of Amomum taso-ko, such as β-eudesmol, fenchol, trans-(-)pinocarveol, δ-cadinene, (-)-myrtenol, and so on, which were not be found in the volatile oils from the seeds. Conclusion: The volatile oils from the shells were about 1/4 of the seeds'. Most of the constituents in the volatile oils from the seeds and shells of Amomum taso-ko are similar, all of the volatile oils contain a higher content of 1,8-cineole and α-terpineol,but still have some difference between the constituents of these two kinds of volatile oils.关键词：Amomum tsao-ko;shell oils;seed oils;GC-MS
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for determining the content of hyperoside, quercitrin and quercetin in Rhododendron Primulaeflorum, and to lay the foundation for the quality control and/or quality evalution of R. primulaeflorum. Method: A high-performance liquid chromatography equipped a Welch Ultimate XB-C18 (4.6 mm×250 mm, 5 μm) column with UV detection was used. The mobile phase consisted of acetonitrile and methanol (5:1)(A) and 0.1% formic acid in water (B) with gradient elution. The column temperature was kept at 30℃ and the flow rate was 1.0 mL·min-1. The detection wavelength was set at 350 nm. Result: The good separation of three compounds was achieved within 70 min. The linear range of hyperoside, quercitrin and quercetin were 4.41-88.20 mg·L-1 (r=0.999 6), 2.94-58.80 mg·L-1 (r=0.999 6),1.485-29.70 mg·L-1 (r=0.999 6) respectively. The average recovery were 103.37%(RSD 1.67%), 98.07%(RSD 1.4%), 100.19%(RSD 1.20%) respectively. Conclusion: This method is simple, accurate with good reproducibility and stability. It can be used as effective methods for quality control of R. primulaeflorum.关键词：Tibetan medicine;Rhododendron primulaeflorum Bur.et Franch;determination;hyperosid;quercetrin;quercetin
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发布时间：2024-01-04 - 摘要：Objective: To study the change rule of dynamic accumulation of biennial Scutellaria baicalensis growth and development and active component in Chengde regions. Method: Took samples once every fifteen days from 1 May to 15 October 2008 and recorded the phenophase. Using high performance liquid chromatographic method to measure the content of baicalin, baicalein and wogonin, and using ultraviolet spectrophotometry to measure its flavonoids content. Result: The general growth rule of aerial parts of biennial S. baicalensis in Chengde regions was slow-fast-slow. Which was in vegetative growth two times. The general growth rule of root was fast-slow-fast, which had highest drying rate on 55.31%. The changes of content of baicalin and flavonoids were similar, which the highest content was at middle May, and the data were 17.61%and 42.92%. The changes of content of baicalein and wogonin were similar, which the highest content was at at June, and the data were 2.63% and 0.49%. Conclusion: The highest content of baicalin and flavonoids in biennial S. baicalensis which was grown in Chengde regions was at middle May. The highest content of wogonin and flavonoids was at June. The highest drying rate was in early and middle October.关键词：Biennial;Scutellaria baicalensis;growth and development;active component
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standards of Panacis Ginseng Radix Laterlis. Method: This experiment collected the Panacis Ginseng Radix Laterlis from different areas,which is to observe the characters, identify the quality by microscopy and TLC, examine the total ash,make the content determination by HPLC. Result: The appearance of 10 batches of samples was different when micro-characteristic were clear. The TLC spots of samples have the same color with Ginseng Radix et Rhizoma,ginsenoside Rb1, Re,Rf,Rg1. however,the total ash was 2.50%-4.26%. ginsenoside Rg1, Re, Rb1 has a good linearity within 0.117 -1.750 μg,0.207 -3.100 μg,0.298 -4.470 μg and the average recovery was 94.4%,95.2%,95.7%with RSD 1.99%,1.55%,1.54%. The content of ginsenoside Rg1+ Re in 10 batchs of samples is 0.228%-0.666%, and the content of ginsenoside Rb1 is 0.312%-0.783%. Conclusion: The quality of Panacis Ginseng Radix Laterlis from different areas is considerably different to be established urgently.关键词：Panacis Ginseng Radix Laterlis;microscopy;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: Using the HPLC and atomic fluorescence spectrometry, to analyze notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and the total arsenic content in Panax notoginseng and its two extracting solutions. Method: The chromatographic separation was achieved on an Agilent TC-C18(4.6 mm×250 mm, 5 μm) column with acetonitrile (A)-0.05% phosphoric acid (B) as mobile phase for gradient elution at the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the detection wavelength was set at 203 nm. Treat Panax notoginseng with different extracting manners (unprocessed, traditional decocting and refluxing by ethanol). Run wet digestion process before measuring the fluorescence intensity, and then get the content of total arsenic. Result: The content of total arsenic differs in different extracting manner, unprocessed>traditional decocting>refluxing by ethanol. Conclusion: Panax notoginseng refluxing by ethanol can extract more pharmaceutical components with low total arsenic. The traditional decocting could be replaced by the ethanol way.关键词：Panax notoginseng (Burk.) F. H. Chen;effective components;HPLC;AFS;Arsenic;refluxing by ethanol
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发布时间：2024-01-04 - 摘要：Objective: To establish a gas chromatographic method for the simultaneous determination of thymol and carvacrol in Herba Thyma. Method: The GC system consisted of capillary column(0.25 μm×0.32 mm, 30 m), FID detector, nitrogen as carrier gas, column temperature programmed, the initial temperature of 50℃, detector temperature of 230℃. Result: The content of thymol and carvacrol in 8 batches of samples was determined, thymol and carvacrol were separated well under the chromatographic conditions. The recoveries of thymol and carvacrol were 99.29%(RSD 2.51%), 99.07%(RSD 2.92%). Conclusion: The method was sensitive, accurate and specific, which is suitable for the quality control of Herba Thyma.关键词：gas chromatography;Herba Thyma;thymol;carvacrol;content determination
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发布时间：2024-01-04 - 摘要：Objective: To establish a HPLC method for the determination of Geniposide and Physcion in Compound Longli Capsule. Method: Waters Symmetry C18 analytical column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was consisted of acetonitrile-water (15:85) and methanol-water (95:5) respectively;the flow rate was 1.0 mL·min-1, UV detection wavelength was set at 238, 254 nm respectively. The column temperature was maintained at 25℃. Result: Under the chromatographic conditions used, the calibration curve of geniposide and physcion was linear in the range of 0.493 2-2.959 2 μg (r=0.999 5) and 0.063 4-0.380 4 μg (r=1.000 0) respectively. The average recoveries was 100.4% and 101.3% with RSD 1.9% and 1.7% respectively. Conclusion: The method is accurate, effective and feasible. It can be used for the determination of the content of geniposide and physcion contained in Compound Longli capsule.关键词：Compound Longli capsule;HPLC;geniposide;physcion
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发布时间：2024-01-04 - 摘要：Objective: To establish a new method for determining the content of protopine in Corydalis Rhizome, and set up the limitation standard of protopine through determining the content of the samples from different origions. Method: HPLC method was used. The separation was performed on a Aglient TC-C18 column (4.6 mm×250 mm, 5 μm) with mobile phase of acetonitrile-3% glacial acetic acid solution (contain 0.8% triethylamine) (18:82). The flow rate was 1.0 mL·min-1and the detection wavelength was set at 289 nm. Result: The calibration curves showed good linear regression within test ranges of 0.052-0.52 μg;the recovery was 99.44%. Conclusion: The method established for determining the content of protopine in Corydalis rhizome was accurate, simple and feasible. The content of protopine of the samples from different origions varied from 0.065% to 0.199% with the average content of 0.124%. It is suggested that the suitable content standard of Corydalis rhizoma should be no less than 0.09%.关键词：Corydalis Rhizoma;different origins;protopine;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: A rapid micellar electrokinetic capillary chromatography method for the routine analysis of alpinetin and cardamonin in Alpinia katsumadai Hayata was established. Method: The analysis was carried out using an unmodified fused-silica capillary (75 μm.; total length 31.2 cm;effective length 21 cm), the background electrolyte (BGE) containing 20 mmol·L-1 sodium borate (pH 7.0)-5 mmol·L-1 sodium dodecyl sulfate (SDS)-10% acetonitrile. The applied voltage was 15 kV and detection wavelength was 286 nm. Result: Under the selected conditions, two components were separated completely within 4 min. The linear range was 25.2-251.1 mg·L-1 (r=0.996 2) for alpinetin and 27.9-167.4 mg·L-1 (r=0.999 5) for cardamonin. The average recoveries were 102.4% (for RSD 2.46%) alpinetin and 97.1% (RSD 2.72%) for cardamonin. Conclusion: This method has the advantages of rapidity, simplicity and has been successfully applied to the analysis of alpinetin and cardamonin in Alpinia katsumadai sample.关键词：micellar electrokinetic capillary chromatography;Alpinia katsumadai;alpinetin;cardamonin
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发布时间：2024-01-04 - 摘要：Objective: To establish the quality standard of Dahuoluo Wan. Method: Twelve crude drugs in the prescription were identified by microscope. Radix Angelicae Sinensis, Radix Aucklandiae and Radix et Rhizoma Glycyrrhizae were identified by TLC. Borneolum Syntheticum was identified by GC. Stilbene glucoside was analyzed by HPLC. Result: The microscopic features were easy to find;the spots in TLC chromatograph were clear and the GC spectrum showed the peak correspond to the standard. The negative samples showed no interference. The linear range of stilbene glucoside was 20.68-2 068 ng (r=0.999 9). The average recovery was 97.9%, RSD 1.3%. Conclusion: The methods are specific, reproducible and reliable, which can be used for the quality control of Dahuoluo Wan.关键词：Dahuoluo Wan;quality standard;microscopical identification;TLC;GC;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To develop an HPLC method for determination of hyperin in Shenqiqiangjing Capsule. Method: Diamonsil C18(4.6 mm×250 mm, 5 μm) column was adopted, the mobile phase consisted of acetonitrile-0.1% phosphatic acid (17:83) at a flow rate of 1.0 mL·min-1, the detection wavelength was set at 350 nm and column temperature was set at 31℃. The injection volume was 20 μL. Result: The linear range was 0.120 1-1.201 6 μg (r=0.999 9), the average recovery (n=6) was 99.12%(RSD 1.90%). The test solution was stable in 24 h. Conclusion: The method is sensitive, convenient and accurate, which can be applied in the determination of Shenqiqiangjing Capsule.关键词：hyperin;Shenqiqiangjing Capsule;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective: To establish the RP-HPLC quantitative determination method of uridine and adenosine in Allii Tuberosi Semen. Method: CNW C18-WP column (4.6 mm×250 mm, 5 μm) was applied with water (A)-acetonitrile (B) as mobile phase. The gradient elution condition was 0-20 min, 0%-20% B and the detection wavelength was set at 260 nm. Result: Uridine and adenosine revealed good linear relation from 9.56 to 95.60 ng and from 10.20 to 102.0 ng respectively with correlation coefficient (r) of 0.999 7 and 0.999 6.The average recoveries of them were 98.04% and 101.99% with RSD of 0.99% and 1.44%. Conclusion: This method was simple and accurate with good repeatability and stability, which was suitable for the quality control of Allii Tuberosi Semen.关键词：Allii Tuberosi Semen;uridine;adenosine;quantitative determination
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发布时间：2024-01-04 - 摘要：Objective: To establish an HPLC method for the simultaneous determination of geniposide, andrographolide and dehydroandrographolide in Lianzhi Qinghuo capsules. Method: Geniposide, andrographolide and dehydroandrographolide were separated on ZORBAX SB-C18(4.6 mm×250 mm, 5 μm) column and detected at 254 nm. The mobile phase was methanol-water with gradient elution. The flow rate was 1.0 mL·min-1. Result: Geniposide, andrographolide and dehydroandrographolide were linear within the range of 0.075 8-2.274 0 μg(r=1), 0.046 1-1.383 0 μg(r=1) and 0.040 9-1.227 0 μg (r=1) respectively. The average recovery was 99.73%(RSD 1.4%), 100.51%(RSD 1.2%) and 101.19%(RSD 1.2%). Conclusion: The method is simple, reproducible, and it can be used for the quality control of Lianzhi Qinghuo capsules.关键词：Lianzhi Qinghuo capsules;geniposide;andrographolide;dehydroandrographolide;HPLC
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发布时间：2024-01-04 - 摘要：Objective: To establish a method for determination of plumbm (Pb) and cadmium (Cd) in pericarpium trichosanthis, and the content of plumbm (Pb) and cadmium (Cd) in pericarpium trichosanthis from 5 different origins were determined. Method: Microwave digestion was used to digest the samples and graphite furnace atomic absorption spectrometry was adopted for the content determination of Pb and Cd in samples. Result: The calibration curves were linear in the range of 0.0-80.0 μg·L-1 for plumbum (r=0.999 1);the average recovery was 94.2%(n=5, RSD 1.4%). The calibration curves were linear in the range of 0.0-10.0 μg·L-1 for cadmium (r=0.999 2);the average recovery was 94.0%(n=5, RSD 1.9%). The content of Pb and Cd was 0.814 and 0.057 mg·kg-1 (Anhui), 0.919 and 0.114 mg·kg-1 (Zhejiang), 0.989 and 0.120 mg·kg-1 (Hebei), 0.745 and 0.042 mg·kg-1 (Shandong), 1.244 and 0.089 mg·kg-1 (Jiangsu). Conclusion: Harmful heavy metals have been detected in pericarpium trichosanthis, which is in line with the limitation stated in the Green Trade Standard for Medicinal Plant and Preparations (Pb≤5.0 mg·kg-1, Cd ≤0.3 mg·kg-1). This method is simple, accurate and suitable for the determination of heavy metals in pericarpium trichosanthis.关键词：atomic absorption spectrometry;pericarpium trichosanthis;plumbm;cadmium;determination
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发布时间：2024-01-04 - 摘要：Objective: To determine 2 underivatized amino acids in Compound Gancaosuanan Injection with HPLC-ELSD. Method: The analysis was carried out on a Agilent-ODS C18 (4.6 mm×250 mm, 5 μm) column. The mobile phase was acetonitrile (A)-5.0 mmol·L-1 heptafluorobutyric acid in 0.7% trifluoroacetic acid solution (B) in gradient elution mode(0-5 min, 0%A;5-10 min, 15% A;10-25 min, 35%A). Drift tube temperature was 80℃, gas flow rate was 2.0 L·min-1. The flow rate was 0.8 mL·min-1. Column temperature was kept at 35℃ and the inject volume was 10 μL. Result: The glycine and cysteine hydrochloride were linear in the range of 4.858-29.145 μg and 0.386-2.316 μg. The average recovery was 97.43%, 96.99% respectively. Conclusion: The method is convenient and accurate, which can be used to determine 2 amino acids in Compound Gancaosuanan Injection.关键词：Compound Gancaosuanan Injection;underivatized;gllycine;cysteine hydrochloride;HPLC;ELSD
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发布时间：2024-01-04 - 摘要：Objective: To compare pharmacokinetics of danshensu(DSS) and ferulic acid(FA) in different combinations to GuanxinⅡ,and to reveal the effect of the combination to GuanxinⅡ on the process in vivo of the two main effective components. Method: Beagle dogs were administrated orally with Guanxin Ⅱin combination with different drugs:total extraction recombination,part extraction recombination propitious to blood,danshen extraction recombination, part extraction recombination propitious to breath,ligusticum wallichii extraction recombination.The plasma concentration of DSS and FA were assayed by LC-MS/MS after administration.the pharmacokinetic parameters were estimated by DAS 2.0 phamacokinetic software edited by SUN Rui-yuan,and then to compare pharmacokinetic parameters within different groups by statistic software SPSS 10.0. Result: The statistic results have showed that the pharmacokinetics of combination with different drugs to GuanxinⅡhave no significant difference,but the tendency. Conclusion: The process of danshensu and ferulic acid in vivo (Beagle dogs)are not significantly different.关键词：Guanxin Ⅱ;pharmacokinetics;combination;danshensu;ferulic acid
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发布时间：2024-01-04 - 摘要：Objective: To develop a UPLC-MS-MS method to simultaneously determine the concentration of ferulic acid, vanillic acid, peoniflorin and albiflorin in beagle plasma and study the pharmacokinetics of these four components after ig administration of Angelicae Sinensis Radix-Paeoniae Radix Alba drug pair extract to beagle dogs. Method: Following a protein precipitation with methanol for plasma samples at different time after ig Angelicae Sinensis Radix-Paeoniae Radix Alba drug pair extract to Beagle dogs. The analysis was achieved by BEH C18 column and the mobile phase was consisted of water (0.1% formic acid included) and acetonitrile followed step gradient elution. A TQ tandem mass spectrometry equipped with electrospray ionization source was used as detector and operated by multiple reaction monitoring (MRM). The ion pairs for quantitation were m/z 195.3-144.5 for ferulic acid, m/z 169.0-92.9 for vanillic acid, and m/z 481.3-197.1 for albiflorin in positive ion mode and m/z 525.4-449.2 for peoniflorin in negative ion mode. The pharmacokinetic parameters were calculated by DAS 2.0 program. Result: Ferulic acid, vanillic acid, peoniflorin and albiflorin showed a good liner relationship of 1.13-1 089,1.23-1 080,0.82-787.5, 0.36-337.5 μg·L-1, respectively. The method was validated according to the requirements. The pharmacokinetic characteristics of ferulic acid and vanillic acid were similar, both of their absorption was very fast. Elimination of ferulic acid was fast while the elimination of vanillic acid was slower. The pharmacokinetic characteristics of peoniflorin and albiflorin were very similar, both of their absorption and elimination were slow in beagle dogs. Conclusion: The method is proved to be sensitive, selective, rapid and successfully applied to a pharmacokinetic study of major components of Angelicae Sinensis Radix-Paeoniae Radix Alba drug pair in beagle dogs. This research laid a good foundation for the study of compatibility of Angelicae Sinensis Radix-Paeoniae Radix Alba drug pair.关键词：Angelicae Sinensis Radix-Paeoniae Radix Alba;Paeoniae Radix Alba;ferulic acid;vanillic acid;peoniflorin;albiflorin;pharmacokinetics;UPLC-MS-MS
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发布时间：2024-01-04 - 摘要：Objective: The effects of the Blumea balsamifera oil on percutaneous absorption of albuterol sulfate were studied. Then, a theoretical basis for the application of B. balsamifera oil was proved. Method: The effects of six different penetration enhancer programs on albuterol sulfate transdermal absorption were determined, i e, 0.5%, 1.0%, 2.0% B. balsamifera oil, 1.0% azone, 1.0% B.balsamifera oil jointed with 1.0% azone and black control.Concretely, the contents of albuterol sulfate in receiving fluid were determined by UV spectrophotometer using modified Franz diffusion cell device and the excised skin from guinea pig was taken as exoteric permeability barrier. Result: 0.5%, 1.0% B. balsamifera oil and 1% azone individually salbutamol sulfate transdermal absorption and storage reservoir showed a significant role in promoting (P<0.05). However, the effect of combination of 1.0% B. balsamifera oil and 1.0% azone on percutaneous penetration was lower than its using alone. Conclusion: With the increase of low concentration of B. balsamifera oil, the transdermal absorption of salbutamol sulfate enhanced. However, high concentration of B. balsamifera oil showed no significant difference.关键词：Blumea balsamifera oil;percutaneous absorption;permeability coefficient;permeability multiples
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发布时间：2024-01-04 - 摘要：Objective: To study effects of Persicae Semen on apoptosis and protein expression of endothelial cell in cold stagnation and blood stasis syndrome (HS) and heat stagnation and blood stasis syndrome (RS) rats. Method: Eighty rats were randomly divided into 8 groups including normal control group of HS (HN), model group of HS (HM), Persicae Semen treatment group of HS (HT), Chuanxiong Rhizoma control group of HS (HC), normal control group of RS (RN), model group of RS (RM), Persicae Semen treatment group of RS (RT), Salviae Miltiorrhizae Radix et Rhizoma control group of RS (RD). To establish the two rat models by freezing and injection of carrageenan solution. Extracts of Persicae Semen, Chuanxiong Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma were given to the corresponding group rats.Severn days later, Four hundred organs derived from the eighty rats were detected by histopathology analysis (HE, IHC and TUNEL). The indexes included growth factor VEGF, apoptotic inhibitor Bcl-2, apoptotic induced protein p53 and apoptotic number of vascular endothelial cell (VEC). Result: ①The corresponding blood stasis syndromes appeared in HM and RM rats, while improvements of the two syndromes appeared in HT and RT rats. ② Decreased VEGF expression appeared just in RT rats(P<0.05), while it did not change in HT rats. Increased Bcl-2 expression appeared just in RT rats(P<0.05), while it did not change in HT rats. p53 expression did not change in both HT and RT rats. ③Apoptosis did not change in both HT and RT rats. Conclusion: Persicae Semen proved the blood circulation and suppressed apoptosis of endothelial cell in blood stasis rats, it might be caused Persicae Semen decreased VEGF and increased Bcl-2 expression of endothelial cells关键词：Persicae Semen;cold stagnation and blood stasis syndrome;heat stagnation and blood stasis syndrome;blood circulation disorder;endothelial cell;apoptosis;TUNEL
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发布时间：2024-01-04 - 摘要：Objective: To study the effects of Guifu Lizhong Wan on hippocampus aging in mice deficiency of kidney and spleen model. Method: Forty SD male mice were randomly divided into normal control group, model group, vitamin E group, Guifu Lizhong Wan group, 10 each group. The normal control group was im treated with 5 mL·kg-1saline and ig with 10 mL·kg-1 at 8:00 am,and 2:00 pm, respectively. The other groups were injected hydrocortisone with 25 mg·kg-1and liquid of Rheum palmatum decoction were administered with 4 g·kg-1at 8:00 am, the model group was administered saline with 10 mL·kg-1, vitamin E group was administered vitamin E with 18×10-3g·kg-1 and Guifu Lizhong Wan groups were given Guifu Lizhong Wan with 1.62 g·kg-1 at 2:00 pm, once a day 30 days. Observation of ultrastructure of hippocampus changes; testing the brain hippocampus cells cycles by flow cytometry. Result: In Hippocampus the highest proportion of G1 phase(P<0.05) was found, compared with the model control group, the vitamin E and Guifu Lizhong Wan increased organization organelles with fewer neuron atrophy,and neurons synaptic gap widening, meanwhile the cell aging rate decreased obviously(P<0.05). Conclusion: Guifu Lizhong Wan has an effect on hippocampus aging in mice deficiency of kidney and spleen model关键词：deficiency of kidney and spleen-Yan;Guifu Lizhong Wan;hippocampus of the brain
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发布时间：2024-01-04 - 摘要：Objective: The aim of this study was to explore the effects of Guizhi plus Gegen decoction(GGD) on spacial memory and inflammatory cytokines and microglia in hippocampus of neuroinflammatory mice induced by lipopolysaccharides(LPS) and its probable mechanism. Method: Totally 63 male ICR mice were randomly divided into 5 groups,i ethe normal control group(Control,n=13),the model group(Model,n=13),the low dose GGD group(GGD-low,n=10),the high dose GGD group (GGD-high,n=14),and the Minocycline positive control(Positive control,n=13,0.05 g·kg-1). AD neuroinflammatory model mice were established through intraperitoneally injected with LPS(0.33 mg·kg-1). Mice had been administered by gastrogavage for 4 weeks with low dose GGD(6 g·kg-1)or high dose GGD(12 g·kg-1).Then, Morris water maze were practiced respectively to observe the effects of GGD on spacial memory,and inflammatory cytokines and microglia of hippocampus in neuroinflammatory mice were tested by ELISA and immunofluoresent assay. Result: The escape latency of model mice induced by LPS increased significantly(P<0.05) in Morris water maze.After treated with low dose or high dose GGD,the model mice had evidently shortened their escape latency (P<0.05, P<0.01). Also,LPS increased significantly content of tumor necrosis factor-α(TNF-α) and interleuktin-1β(IL-1β) in hippocampus(P<0.01).Microglia in hippocampus of Model mice are characterized by the rise in the numbers and activated phenomenon such as radial.GGD reduced the content of TNF-α and IL-1β at some degree(P<0.05),and regulated the activated phenomenon of microglia. Conclusion: These results indicate that GGD could improve spacial memory disorder in neuroinflammatory mice induced by LPS.Its mechanism may be possibly correlated with its regulatory effects of GGD on activated microglia and content of TNF-α and IL-1β in hippocampus.关键词：neuroinflammation;spacial learning;inflammatory cytokines;microglia;Guizhi plus Gegen decoction
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发布时间：2024-01-04 - 摘要：Objective: To investigate the antidepressant effect and the possible mechanism of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside(THSG) in animal model. Method: Mail KM mice were randomly divided into 5 groups:control group (normal saline), fluoxetine group (5 mg·kg-1), THSG groups (200,100,50 mg·kg-1). After oral administration for 7 days, the tail-suspending test (TST), the forced swimming test (FST) and reserpine-antagonism test were carried out to evaluate the antidepressant effects of THSG. In particular, reserpine (1 mg·kg-1)was given by intrapertioneal injection before the reserpine-antagonism test. Result: Compared with the control group, the immobility time of TST and FST was shorted in THSG high,and middle,dose group (P<0.01). Compared with the model group, THSG high, middle, dose group could significantly reverse the ptosis, and decrease body temperature induced by reserpine in mice (P<0.01,P<0.05). Conclusion: THSG has significant antidepressant effect, and its mechanism may be related to monoamine neurotransmitters.关键词：2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside;antidepressant effects;forced swimming test;reserpine
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发布时间：2024-01-04 - 摘要：Objective: To investigate the neuroprotective effect of Shengmai injection (SMI) on the expression of tissue factor (TF) and related signal pathways in mice with cerebral ischemia-reperfusion injury. Method: Mice were randomly divided into 5 groups as following:sham operation group, ischemia-reperfusion(I/R) group, SMI groups at three doses (5.68, 2.84, 1.42 g·kg-1). Right middle cerebral artery was occluded by inserting a thread through internal carotid artery for 1 h. The infarct volume and neurological scores were evaluated after 24 h reperfusion. Western blotting was used to determine the expression of tissue factor (TF), nuclear factor-kappa B (NF-κB) subunit p65, Akt, and their phosphorylation after 1 h reperfusion. Result: Compared with I/R group, SMI could significantly reduce the infarct volume and improve the neurological deficits in a dose-dependent manner. SMI also remarkably down-regulated the expression of TF, inhibited the activation of NF-κB induced by ischemia-reperfusion and the phosphorylation of Akt in mice. Conclusion: Shengmai injection, at certain dosage range, elicits potent protection against cerebral ischemia/reperfusion injury, which may partly be due to inhibiting TF expression and its related pathways.关键词：Shengmai Injection;cerebral ischemia/reperfusion;tissue factor;nuclear factor-κB;Akt
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发布时间：2024-01-04 - 摘要：Objective: To observe the anti-inflammatory and analgesic effects of Xiaoaishu(XAS) and explore it's mechanism. Method: The KM mice were randomly divided into model group, aspirin group(0.2 g·kg-1) and XAS groups (0.375,0.75,1.5 g·kg-1) ig, for 7 d. Carrageenan-induced paw swelling and dimethylbenzene-induced pinna swelling in mice were used to evaluate the anti-inflammatory effects. The analgesic effects of XAS were tested by counting writhing frequency induced by acetic acid and measuring the latent period of licking hind foot with the hot plate method in mice.The levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) mRNA was determined by real time RT-PCR. Result: XAS significantly suppressed the dimethylbenzene-induced mice auricle swelling and Carrageenan-induced mice paw edema(P<0.05),XAS significantly reduced the acetic acid-induced writhing frequency and pain threshold in mice(P<0.05),real-time RT-PCR showed that XAS could decrease the level of TNF-α and IL-1β mRNA of inflammatory tissue in mice (P<0.05). Conclusion: XAS have evident anti-inflammatory and analgesic effects, the mechanism may be related with the inhibition of the expression of TNF-α and IL-1β mRNA in the carrageenen-induced mice paw.关键词：XAS;anti-inflammatory;analgesia;cytokines
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发布时间：2024-01-04 - 摘要：Objective: To study the effects and mechanism of momordica grosvenori flavones (MGF) on myocardial energy metabolism and expression of peroxisome proliferators-activated receptor α(PPARα) mRNA in exercise rats. Method: Fifty healthy male Sprague-Dawley rats were randomly divided into five groups (n=10):quiet control group (NC), exercise control group (ME), exercise+ig low dose flavones group (MGFL), ig moderate dose flavones group (MGFM) and intragastric gavage (ig) high dose flavones group (MGFH). All rats in administrated groups were intragastric gavaged in profession once time at half an hour before exercise. The administration was continued for 6 d/week in total 6 weeks at the dose of 100, 200, 400 mg·kg-1 in low, medium and high dose group respectively, while the rats in unadministrated groups were treated by physiological saline solution according to the same dose. The rats were killed immediately by decapitation at the end of training. The activities of creatine kinase (CK), lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) in myocardial tissue were measured according the corresponding kit method respectively. The expression of PPARαmRNA in myocardial tissue was determined reverse transcription polymerase chain reaction (RT-PCR). Result: The activity of myocardial CK was decreased in rat by exhaustive swimming exercise. It was significantly lower in exercise control group than that in quiet control group (P<0.01). There was significant difference in low dose MGF group compared with quiet control group (P<0.05). The activity of myocardial CK was obviously higher in MGF groups than that in exercise control group (P<0.01). The activity of LDH was significantly lower in exhaustive swimming groups than that in quiet control group (P<0.01). It was higher in each MGF group than that in exercise control group (P<0.05 or P<0.01). The activity of SDH was obviously lower in exhaustive swimming groups than that in quiet control group (P<0.01). It was lower in low dose MGF group than that in quiet control group (P<0.05), while it was higher in moderate, and high dose MGF groups than that in exercise control group (P<0.05). The expression of PPARα mRNA was obviously lower in exercise control group than that in quiet control group (P<0.01). It was higher in MGFL group than that in exercise control group (P<0.05). The expression of PPARα mRNA in MGFM and MGFH groups were significantly higher than that in exercise control group (P<0.01). Conclusion: Supplement of momordica grosvenori flavones could improve the activities of energy metabolic enzymes CK, LDH and SDH, and upregulate the expression of PPARα mRNA. This has a good role in improving the energy metabolism and protecting the myocardial tissue from overuse exercise injury.关键词：momordica grosvenori flavones;exhaustive exercise;myocardial tissue;energy metabolic enzyme;PPARα mRNA
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发布时间：2024-01-04 - 摘要：Objective: To study the positive inotropic effect of higenamineand 6-gingerol and their compatibility on normal cardiac myocytes. Method: The left ventricular cardiac myocytes were isolated from normal rat by traditional Langendroff method. The cardiac myocytes contraction were detected by using different monomers and their compatibility (Ion Optix cell dimensioning systems). The detections included the maximum velocity of systolic and diastolic (±dp/dt), contraction amplitude (ph/bl) and the time course constant of 50% systolic and diastolic (T50S, T50D). Result: 0.03-1 μmol·L-1 higenamine enhances±dp/dt, ph/bl with a dose- dependent manner, but not influenced the T50S and T50D. The minimum effective concentration was 0.1 μmol·L-1, EC50 was approximately 0.205 μmol·L-1, and 1 μmol·L-1 could get the maximum efficacy. The maximum efficacy of ph/bl,+dp/dt,-dp/dt was up to 234%, 219% and 272%. It's could induce the arrhythmia contraction when the dose was higher than 1 μmol·L-1. 3-30 μmol·L-1 6-gingerol had not any effects of contractility on cardiac myocytes. However, the higher concentrations than 30 μmol·L-1 also induced the arrhythmia contraction. This research also investigated the 3, 10 μmol·L-1 6-gingerol compatibility with 0.1 μmol·L-1 and 0.3 μmol·L-1 higenamine. Mutual compatibility could not enhance the positive inotropic effect of higenamine. Conclusion: Higenamine plays the positive inotropic effect,and 6-gingerol has nether any effects on monomer, nor compatibility with higenamine.关键词：higenamine;6-gingerol;compatibility;positive inotropic;cell contractility
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发布时间：2024-01-04 - 摘要：Objective: To study the influence of Atractylodis Macrocephalae Rhizoma and Atractylodis Macrocephalae Rhizoma fried with bran on somatostatin (SS), gastrin (GAS), cholinesterase (CHE)in rat spleen deficiency model. Method: The rats were divided into five groups, namely blank group, Atractylodis Macrocephalae Rhizoma raw group, Atractylodis Macrocephalae Rhizoma fried with bran group, positive drug group, model group, 10 rats each group. Subcutaneous injection of reserpine was used to make rat spleen deficiency model, after modeling, the serum was separated, CHE activity, SS and GAS content were assayed. Result: In the model group, the content of SS,GAS in the serum was significantly reduced,CHE activity increased. The crude Atractylodis Macrocephalae Rhizoma and Atractylodis Macrocephalae Rhizoma with bran fried could decrease CHE activity;SS, GAS content increased compared with the model group. Conclusion: The raw Atractylodis Macrocephalae Rhizoma and Atractylodis Macrocephalae Rhizoma fried with bran have a better therapeutic effect.关键词：Atractylodis Macrocephalae Rhizoma;Atractylodis Macrocephalae Rhizoma fried with bran;spleen deficiency;somatostatin;gastrin;Cholinesterase
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发布时间：2024-01-04 - 摘要：Objective: To compare the effects of different processed products of Rehmanniae Radix in Siwu prescription, and the difference between raw rehmannia and prepared rehmannia. Method: Kunming female mice were injected, on day 1, 2, 3 respectively celiac injection (ip) cyclophosphamide (CTX) 80 mg·kg-1 to establish immunosuppression model. After 7 days of administration, the spleen and thymus, acquisition lymphocyte sample were collected. Flow cytometry instrument was used to test CD3+,CD4+,CD8+. Result: The prepared Rehmannia Radix of Siwu prescription could improve the immune suppression of spleen and thymus index (P<0.05), and at the same time, improve CD3+,CD4+, CD8+lymphocytes (P<0.01) and CD4+/CD8+ Conclusion: The prepared Rehmannia Radix of Siwu prescription can improve the T cell subsets. Rehmanniae Radix with different processed methods has an influence on the immune function.关键词：different processed product of Rehmanniae Radix;cyclophosphamide;immunosuppression;lymphocyte subsets
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Hawthorn leaves flavonoids self-emulsifying granules (HLFSG) on non-alcoholic fatty liver disease. Method: Forty rats were randomized into 4 groups and fed high fat diet to induce non-alcoholic fatty liver disease model. Rosiglitazone was as the positive control group. The influence of the HLFSG(50 mg·kg-1)treatments on total cholesterol(TC), triglyceride(TG), low-density lipoprotein-C(LDL-C), high-density lipoprotein-C (HDL-C), alanine aminotransferase(ALT), aspartate aminotransferase(AST), free fatty acid(FFA), superoxide dismutase (SOD), malondialdehyde(MDA) was investigated. Result: Compared with the model group,HLFSG reduced TC, TG, LDL-C, FFA, ALT,AST, MDA significantly (P<0.05,P<0.01), and a significant increase of HDL-C and SOD was observed. Conclusion: HLFSG is beneficial to the treatment of fatty liver through reducing blood lipid and improving the liver functions in rats.关键词：Hawthorn leaves flavonoids self-emulsifying granules;nonalcoholic fatty liver;rat
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发布时间：2024-01-04 - 摘要：Objective: To explore the mechanism and the therapeutic effects of Shenfu injection (SF) on the rat models with buerger's disease or thromboangiitis obliterans (TAO). Method: Adult male Sprague Dawley rats were randomly divided into sham operated group, TAO model group, SF low dose (2.5 mg·kg-1), medium dose (5 mg·kg-1) and high dose (10 mg·kg-1) groups (n=8). Rats were intravenously administered SF 2.5, 5, 10 mg·kg-1 or saline once per day for 15 days. TAO model was prepared by injecting sodium laurate into the femoral artery of rats. Then we examined the alterations of pathological signs, pathologic grading of thrombus, blood platelet count, the contents of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-K-PGF1α) in plasma following SF or saline treatment. Result: More physiological signs of lesions and higher grades of pathological thrombosis, the increase in the TXB2 content, blood platelet count and the decrease of 6-K-PGF1αcontent in TAO model group were observed in our experiments;Compared with TAO model group, SF treatment significantly improved the physiological signs of lesions induced by sodium laurate injection and reduced the numbers of thrombus formation and the TXB2 content, as well as blood platelet count but increased the 6-K-PGF1αcontent. Conclusion: These results first suggest that SF can cause a significant therapeutic effect on experimental TAO model rats by its inhibiting platelet aggregation and enhancing anti-thrombus function of vessel endothelia.关键词：thromboangiitis obliterans;Shenfu Injection;platelet aggregation;thrombus
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发布时间：2024-01-04 - 摘要：Objective: To study the sedative and hypnotic effects of Gandan Liangyi Tang (GDLYT) and the potential mechanism in mice. Method: The KM mice were divided into seven groups:control group, diazepam group, Bailemian group, GDLYT 5.2, 2.6, 1.3, 0.65 g·kg-1 dose groups, and mice were administrated once per day. The threshold dose of pentobarbital sodium was observed in mice. The locomotion activity was estimated, and the rotarod test was used to evaluate the effects of GDLYT. The contents of amino acids neurotranmitters including aspartic acid (Asp), glutamic acid (Glu), glycine (Gly), γ-aminobutyric acid (GABA) in mice brain were determined by HPLC-FL. Result: The results showed that administration of GDLYT at 2.6, 1.3 g·kg-1·d-1 significantly shorted the latency period and lengthened the duration of sleep(P<0.05 or P<0.01). Besides, the locomotion activities were significantly reduced by GDLYT (1.3, 2.6 g·kg-1·d-1)(P<0.05 or P<0.01)while the coordination of motion was no changed in mice after GDLYT treated. The results of HPLC indicated that GDLYT 5.2 g·kg-1group can significantly increase the GABA, Asp and the Glu content(P<0.05 or P<0.01), and the 2.6 g·kg-1group could increase the GABA and Gly content significantly(P<0.05 or P<0.01), meanwhile the 1.3 g·kg-1 group could significantly increase the content of GABA (P<0.01)in the mice brain tissues. Conclusion: Our results suggested that GDLYT exerts sedative and hypnotic activities, and the mechanisms may be correlated with the effects of increasing the contents of amino acids neurotranmitters GABA and Gly.关键词：Gandan Liangyi Tang;sedative hypnotic;central neurotrasmitter
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发布时间：2024-01-04 - 摘要：Objective: To explore the impact of Qigong Kangxian prescription(QF) on Smad 2/3, Smad7 in hepatic fibrosis rats. Method: Sixty SD rats randomly divided into 6 groups, in addition to the normal control group, the other groups were intraperitoneally injected with 0.5 mL pig's serum twice per week for 8 weeks, the rat hepatic fibrosis model was induced. Rats were given corresponding herbal medicines. After the end of experiment, serum samples were collected. Determination of the content of transforming growth factor was carried out by using ELISA method. Sections of liver tissue was fixed in 10% formaldehyde solution,and then embedded in paraffin, Under the light microscope. Expression of liver tissue TGF-β1,Smad2, Smad3, Smad7 was detected by immunohistochemical method. Result: In the Content of TGF——β1 and expression of liver tissue TGF-β1,Smad2,Smad3 and Smad7 the significant differences were found in administration groups compared with the model group(P<0.01). Conclusion: QF can suppress hyperplasy of hepatic fibrous connective tissue, lessen hepatic injury through decreasing Smad 2/3 expression and increase Smad7 expression.关键词：Qigong Kangxian open the prescription;promoting blood-flow group;softening hard-mass group;hepatic fibrosis;Smad 2/3;Smad 7
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发布时间：2024-01-04 - 摘要：Objective: To observe effects of orexin on wakefulness and content of norepinephrine(NE),serotonin(5-HT) in hypothalamus to discuss the mechanisms. Method: Rats were divided into the control group,the model group,the low-dose group and the high-dose group at random. Each group included 10 rats.The anesthesia rat model was established with intraperitoneal injection by using chloral hydrate and urethane mixture(1:1)according to 5 mL·kg-1. The low-dose group were intraperitoneally injected orexin 10 μL (1 mmol·L-1,0.1 mg)from paracele and the high-dose group were intraperitoneal injected orexin 10 μL (5 mmol·L-1,0.5 mg)from paracele. To observe lost of righting reflex(LORR) time and LORR lasting time. ELISA method was applied to test content of NE,5-HT in hypothalamus. Result: Compared with the control group, in the model group the content of both NE[(82.44±2.16) ng·L-1] and 5-HT[(149.88±14.21) ng·L-1] declined, but there was no significant difference. Compared with the model group by content of NE in the high-dose group the content of NE[(158.83±4.87) ng·L-1] increased significantly(P<0.01).Compared with the model group by content of 5-HT [(149.88±14.21) ng·L-1], in the low-dose group the content of 5-HT [(232.58±16.05) ng·L-1] increased (P<0.05) and in the high-dose group the content of 5-HT [(278.14±17.85[BFQ])ng·L-1] increased significantly(P<0.01). Compared with the model group by LORR lasting time [(61.08±11.32) min], in the high-dose group [(32.01±7.42) min] reduced significantly(P<0.01). Conclusion: orexin could excite NE,5-HT neuron, which enhance cerebral cortex effect of ‘facilitatioin’ on monamine neuron,finally may cause and keep wakefulness, which is probably one of the mechanisms of orexin causing wakefulness.关键词：orexin;NE;5-HT;wakefulness
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发布时间：2024-01-04 - 摘要：Objective: To compare the antioxidant activity of emodin and 3 emodin metal complex. Method: Compound the 3 emodin metal complex,then use the UV spectrum,study the clearance rate of emodin and 3 emodin metal complex to DPPH free redical,O2-·free redicals and ·OH free redical. Result: For·OH free redical, the Concentration for 50% of Maximum Effect(EC50) of emodin-ferrum reaches 19.96 mg·L-1,this numerical value is the smallest one of all,So emodin-Ferrum the best one.For the same reason, the antioxidant activity for O2-·free redicals of emodin-Ferrum is the best. Conclusion: The antioxidant activity enhance after forming metal complex,and emodin-Ferrum is the best one.关键词：emodin;metal complex;antioxidant activity
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发布时间：2024-01-04 - 摘要：Objective: To study the anti-fatigue action of different extracts from Portulaca oleracea in senile mice by D-gal, and its mechanisms. Method: The mice were randomly divided into 4 groups:control group, model group, the n-butanol extracts from P. oleracea(0.5, 0.25 g·kg-1) groups and the ethanol extracts from P. oleracea(1,0.5 g·kg-1) groups. The control group is given hypodermic injection of isotonic NaCl in the transverse part while the other groups successively administered by hypodermic injection of D-gal(1 g·kg-1). It is made a model of senile mice after 42 d continuation. To measure anti-fatigue performances with the Fatigue Instrument. To measure malondialdehyde(MDA) contents and superoxide dismutase(SOD) activity in brain tissue of senile mice. Result: The n-butanol extract from P. oleracea (0.5 g·kg-1) and the ethanol extract from P. oleracea(1 g·kg-1) could improve anti-fatigue performances.The n-butanol extracts from P. oleracea(0.5, 0.25 g·kg-1) could decrease MDA contents and SOD activity in brain tissue. The ethanol extract from P. oleracea (1 g·kg-1) could decrease MDA contents n brain tissue. Conclusion: The n-butanol extract from P. oleracea and the ethanol extract from P. oleracea can improve anti-fatigue performances. Its mechanism maybe related by improve SOD activity and decrease MDA contents. The n-butanol extract from P. oleracea is better than the ethanol extract from P. oleracea.关键词：Portulaca oleracea;senile;anti-fatigue;SOD;MDA
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of Renshen Jianxin (RSJX) capsule on neuroendocrine activation in rat chronic congestive heart failure (CHF). Method: The rat CHF model was established by intraperitoneal injection of adriamycin(ADM) once a week for 6 weeks. From the fifth week rats were given cold herbal compound for 7 days. Fifty Wistar rats were divided into five groups at random including:normal group, model group, captopril group, low dose and high dose.Groups of RSJX after 2 weeks, the circulatory level of nitric oxide (NO), endothelin (ET), angiotensin Ⅱ (AngⅡ), interleukin (IL)-6, tumor necrosis factor-α(TNF-α) in blood of rats, cardiac index and cardiac muscle pathology were analyzed. Result: Compared with normal group, the heart weight(HW)(P<0.05), left ventricle weight (LVW)(P<0.01), index of LVW (LVW/BW) and HW (HW/BW) of model group were increased obviously(P<0.01). Compared with model group, RSJX capsule could reduce LVW/BW (P<0.01) and HW/BW (P<0.01),decrease the circulatory level of ET (P<0.01), IL-6 (P<0.01), AngⅡ(P<0.01) and NO (P<0.01), and the high dose group was better than the low dose group (P<0.01). The pathological section of cardiac muscle showed that RSJX capsule could protect the cardiac muscle by improving the degeneration of the cardiac cell and reducing the inflammatory cell infiltration. Conclusion: RSJX capsule can improve the left ventricular hypertrophy in CHF rats caused by ADM with the possible mechanism of regulating the neuroendocrine activation and also can protect cardiac damage caused by ADM.关键词：chronic congestive heart failure;Renshen Jianxin capsule;doxorubicin;neuroendocrine factors;Pathology
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发布时间：2024-01-04 - 摘要：Objective: To explore the mechanism of Dihuang Yinzi in ameliorating the damage induced by cerebral ischemia-reperfusion. Method: Sixty SD rats were selected and randomly divided into six groups:sham group, model group, Dihuang Yinzi high, medium,low dose group(32,16,8 g·kg-1) and nimodipine positive drug group(1 mg·kg-1),each group include 10 rats. The cerebral ischemia-reperfusion model was established after 7 days drug administration. The activities of superoxide dismutase(SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and the content of malonaldehyde(MDA) in the serum and brain of rats were observed at 120 min after the cerebral ischemia-reperfusion. Result: The activitis of SOD,CAT and GSH-Px were lower and the contents of MDA were higher in serum and brain of mode1 rats than normal rats.Dihuang Yinzi could increase the activitis of SOD,CAT and GSH-Px and decrease the content of MDA. Conclusion: Dihuang Yinzi can ameliorate the oxidative damage induced by cerebral ischemia-reperfusion.关键词：Dihuang Yinzi;cerebral ischemia;reperfusion injury;antioxidant
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the toxicity effect of raw Radix Aconiti and raw Rhizoma Pinelliae on lactation rat's myocardial cells and the relationship between the toxicity effect and different proportion. Method: The SD lactation rat's myocardial cells were separated and cultivated when born in 3 days. The four groups were raw Radix Aconite, Radix Aconiti and raw Rhizoma Pinelliae 1:0.25, 1:0.5,1:1(Ⅰ,Ⅱ,Ⅲ,Ⅳ)were given the final concentration of the Radix Aconiti of 0,0.016, 0.078, 0.235, 0.392, 0.548, 0.705, 0.861, 1.018 g·mL-1·L-1] to the myocardiaL cells, respectively cultivating 1, 2, 4, 12 h, to observe the cell pulsation frequency in different time, with MTT method to detect the percentage of the cell survival at 12 hours,and calculate half inhibition concentration (IC50)by probability unit method. Result: ①At 12 h,the inhibition rate (CI) of raw Radix Aconiti group was significantly higher than other groups (P<0.01), and with the increasing of theproportion of the raw pinellia tuber,the inhibition rate was decreased. ② The IC50 ofⅠ, Ⅱ, Ⅲ, Ⅳ group were 0.446, 0.460, 0.530, 0.575 g·mL-1 ③ When the final concentration of raw Radix Aconiti is 0.016-0.078 g·mL-1, the cell pulsation frequency was increased in 1-2 h, and reduced in 4-12 h. Between 0.235-0.861 g·mL-1, the cell pulsation frequency was reduced in 1 h,and rised when 2 h,but less than normal level,then falled in 4-12 h.Dose with 1.018 g·mL-1, the cells were immediately arrested. Conclusion: The addition of Raw pinellia reduced the toxicity of the raw Radix Aconiti, and there is a significant dose-response relationship.关键词：Myocardial cells;raw Radix Aconiti;raw Rhizoma pinelliae;IC50;pulsation frequency
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发布时间：2024-01-04 - 摘要：Objective: To observe the antithrombotic effects of Polygonum amplexicaule decoction and the action of mechanism. Method: After orally given different doses of P. amplexicaule decoction(crude drug dose:0.4,0.2,0.1 g·kg-1) for 7 days, the clotting time of each group mice was determined; thrombus induced by collagen-epinephrine were established, positive control group was orally given 0.5 g·kg-1 Xueshuan Xinmaining tablets,experimental drug group were orally given different doses of P. amplexicaule decoction(crude drug dose:0.6,0.4,0.2 g·kg-1), model group was given equal volume normal saline once a day for 7 d, then thrombosis-caused hemiplegia and death of each group were determined. Experimental models of artery thrombosis was prepared, they were given P. amplexicaule decoction by intragastric administration for 15 days. Then the blood was taken, the content of plasma thromboxane B2(TXB2), 6-keto-prostaglandin F1α(6-Keto-PGF1α), tissue-type plasminogen activator(t-PA) and plasminogen activator inhibitor(PAI) were determined. Effect of P. amplexicaule decoction on antiplatelet aggregation induced by ADP, AA and COL were also investigated. Result: The hemostasis time of mice after given high, middle and low doses of P. amplexicaule decoction was (164±23), (143±20), (112±21)s, P. amplexicaule decoction could prolong the clotting time of mice; mortality data of thrombotic mice after given high, middle and low doses of P. amplexicaule decoction was 1,2,2,and hemiplegia data of that was 2,2,3, compared with the model group there was significant difference; the content of TXB2, 6-Keto-PGF1α, t-PA of blood-stasis model rats could reduce after given P. amplexicaule decoction, content of PAI increased, and inhibite platelet aggregations. Conclusion: P. amplexicaule decoction has obvious antithrombotic effects, which was probably related to platelet functions and aggregations.关键词：Polygonum amplexicaule;decoction;antithrombotic;mechanism
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发布时间：2024-01-04 -
Protective Effect of Chinese Herbal Compound Extract on Damage Induced by Cigarette Mainstream Smoke
摘要：Objective: To study the effect of Chinese herbal compound extract on cigarette mainstream smoke induced damage. Method: The effect of cigarette mainstream smoke on cytotoxicity toward Chinese hamster ovarian cells(CHO cells) was detected.Salmonella mutagenicity test, micronucleus test, proliferation of human bronchial epithelial cell line (BEP2D) cells in vitro were also employed,peripheral blood cells, ammonia induced cough, phenolsulfanphthalein excretion test in vivo were carried out after animals were exposed to cigarette smoke for 30 days. Intervention effects of Chinese herb compound on all these tests were evaluated after added in cigarette at 0.03%. Result: After added in cigarette at 0.03%,Chinese herbal compound extract did not increase thesalmonella mutagenicity and micronucleus frequency and could reduce the cytotoxicity toward CHO cells induced by cigarette smoke. Chinese herbal compound extract could reduce the injury of human respiratory epithelial cells. For the research that animals were exposed to cigarette smoke for 30 days, Chinese herbal compound extract (2 cigarette·d-1) could inhibit the decrease in hemoglobin (P<0.05);4 cigarette·d-1could inhibit the increase in platelet count and the increasing of times of cough (p<0.05);8 cigarette·d-1 could reverse the shortenedlatent period of cough (P<0.05),and reverse the decrease of excretion of phenolsulfonphthalein (P<0.05). Conclusion: Chinese herbal compound extract doesn't increase the in vitro cytotoxicity of cigarette smoke and even shows reducing effect or tendency, Chinese herbal compound extract could reduce the injury of cigarette smoke by reducing the cough, thick sputum and change in peripheral blood cells induced by smoking, and though directly protective effect on respiratory epithelial cells.关键词：Chinese herbal compound extract;cigarette smoke;cytoprotection;antitussive expectorant;cytotoxicity in vitro- 2
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of water extracts from Momordica charantia on blood lipid in mouse model of hyperlipidemia. Method: The hyperlipidemia mouse model was induced by high fat diet successfully and the mice were randomly divided into 4 groups:high(30 g·kg-1) and low(10 g·kg-1) dose group of water extracts from M. charantia,L-carnitine(1 g·kg-1)and model group, 10 mice in each group. Another 10 normal mice were used as the control group. After treatment of 4 weeks by gastric gavage, serum total cholesterol (TC)and triglyceride(TG)levels were measured by automatic biochemical analyzer. Liver index was calculated and pathological changes of liver tissue were observed by hematoxylin-eosin(HE) staining. Result: Serum TC, TG and liver index of drug groups were significantly reduced after treatment. TC, TG and liver index in high dose group was (2.78±0.33), (0.90±0.13) mmol·L-1,(0.034±0.002) g·g-1;those in low dose group was (3.87±0.08), (1.03±0.09) mmol·L-1 and (0.040±0.005) g·g-1. Compared with model group of (4.86±0.34), (1.43±0.06) mmol·L-1and (0.057±0.007)g·g-1, there were statistical differences (P<0.01,P<0.05). The HE staining results showed that water extracts from M. charantia could significantly reduce the liver fat content of hyperlipidemic mice. Conclusion: Water extracts from M. charantia can evidently decrease blood lipids.关键词：water extracts from Momordica charantia;hyperlipidemia;antilipemic effect
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发布时间：2024-01-04 - 摘要：Objective: To study the protective effect of the total flavonoids of bidens (TFB) on the D-GalN induced acute liver injury in mice. Method: Acute liver injury in mice was established by intraperitoneal injection of D-GalN. The mice were randomly divided into five groups:model group, bifendate group (120 mg·kg-1), low, medium, high-dosage groups of TFB (60, 120, 240 mg·kg-1). A normal control group was set up also. The index of thymus, spleen and liver were measured. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP), serum contents of albumin (Alb) and total antioxidant capacity (T-AOC) were examined using colorimetric detection. And the expression of inducibility nitric oxide synthase (iNOS) in liver tissue was determined by western blot analysis. Pathomorphological changes in microscopic slodes were observed by hematoxylin-eosin staining (HE). Result: Compared to model group, TFB markedly increased the index of thymus, spleen, and liver (P<0.01), and lowered the serum levels of ALT, AST and AKP (P<0.01), while elevated the contents of Alb, T-AOC (P<0.01), down-regulated the expression of iNOS (P<0.01), and alleviated the liver damage condition. Conclusion: The total flavonoids of bidens has protective effect on the D-GalN induced liver injury in mice. The mechanism may be related to antioxidation and inhibition of cytotoxicity.关键词：total flavonoids of bidens;liver injure;Antioxidation;iNOS
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发布时间：2024-01-04 - 摘要：Objective: To study the effect and mechanism of CsB and its effective components on liver fibrosis in rats induced by dimethylnitrosamine (DMN). Method: From the 1st to 4th week, rats were treated with intraperitoneal injection of DMN (5 mg·kg-1) for three continuous day each week. After modeling, normal group and model group were given saline (10 mL·kg-1) intragastrically, and rats in drug treatment group were treated separately with CsB and its components C12 (800 mg·kg-1·d-1 and 5 mg·kg-1·d-1) for 2 weeks. After all courses of treatment, all rats were sacrificed to harvest blood and liver tissue sample.Serum alanine aminotransferase(ALT), aspartate transferase (AST), albumin (Alb), total bilirubin (TBil) and content of hydroxyproline (Hyp)in liver tissue were detected. Histological changes with HE staining and collagen deposition with sirus red staining in rat liver tissue were observed. Expression of hepatocyte growth factor (HGF-α) and tumor necrosis factor-α (TNF-α) were detected immunohistologically. Result: Compared with 6 weeks model group, serum ALT and AST activity, TBil content and Hyp content in liver tissue in CsB and C12 group lowered and Alb content increased significantly (P<0.05). Compared with 6 weeks model group,some histological improvement was made in CsB and C12 group. Compared with the 6 wk model group, HGF-α protein expression in liver tissue in CsB and C12 group increased significantly(P<0.01). Compared with 6 weeks model group, quantity apoptotic hepatocytes in Cs and C12 group decreased obviously (P<0.01). Conclusion: CsB and C12 can block development and formation of liver fibrosis induced by dimethylnitrosamine, by protecting hepatocytes to resist liver injury, decreasing synthesis of collagen, inhibiting oxidative stress. CsB and C12 can improve the level of expression of HGF-α and inhibit hepatocytes apoptosis to balance apoptosis and proliferation, so as to block or reverse development liver fibrosis.关键词：liver fibrosis;CsB;effective components;HGF-α;hepatocytes apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the antioxidative activity of total flavones of Kummerowia striata. Method: The total flavones of K. striata were extracted by 75% ethanol and purified by polyamide resin. And the contents of total flavones were measured. The antioxidative activity of total flavones of K. striata were analyzed by using different assays as 1,1-diphenyl-2-picryhydrozyl radica 12,2-diphenyl-1-(2,4,6-trintrophenyl) hydrazyl (DPPH) radical scavenging, diammonium 2,2'-agino-bis(3-ethylbenzothiazoline-6-salfonate(ABTS+) radical scavenging, superoxide radical scavenging, hydroxyl radical scavenging, reducing power and chelating ability, with the positive control of the antioxidative activity of butgl hydroxy anisd(BHA), trolox and citric acid. Result: The contents of total flavones of K. striata were (3.26±0.36)%(n=3),with strong free radical scavenging activity, effective reducing power and chelating ability. The IC50 of inhibition for DPPH radical, ABTS+radical, superoxide radical, hydroxyl radical, reducing power (Fe3+), reducing power (Cu2+), chelating ability (Fe2+) and chelating ability (Cu2+) were 7.84,2.384,26.48,1.878,24.67,7.67,77.67,70.15 mg·L-1 respectively. And all were related to the dose. Conclusion: The total flavones of K. striata has obvious antioxidative activity.关键词：Kummerowia Striata;total flavones;antioxidant;free radical;clearance
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发布时间：2024-01-04 - 摘要：Objective: Effect of tanshinone ⅡA on homocysteine (HCY) induced proliferation of vascular smooth muscle cells (VSMC) of endoplasmic reticulum stress (ERS) gene of immunoglobulin heavy chain binding protein (Bip) expression, to explore the mechanism of tanshinone ⅡA in atherosclerosis. Method: To establish the proliferation of rabbit VSMC model induced by HCY, incubated with different concentration of tanshinone ⅡA, Annexin/PI double staining, in situ nick end labeling (TUNEL) method to detect apoptosis rate, to observe the morphology of cell apoptosis; real-time fluorescent quantitative PCR detection the expression of Bip gene. Result: Tanshinone ⅡA can significantly promote the apoptosis of rabbit VSMC, cell apoptosis rate compared with HCY group (P<0.01), and there was a dose dependent; expression levels of tanshinone ⅡA group Bip mRNA were significantly increased, compared with the HCY group had significant difference (P<0.05, P<0.01). Conclusion: Expression of tanshinone ⅡA up-regulation of Bip gene, amplified ERS signal, induce apoptosis of rabbit VSMC, which may be one of the mechanisms of atherosclerosis.关键词：tanshinoneⅡA;vascular smooth muscle cell;homocysteinemia;immunoglobulin heavy chains binding protein;cell apoptosis
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Upper middle lower generic gout decoction in the treatment of hyperuricemia. Method: Hyperuricemia rat model was established by administration of adenine and ethambutol, ig, for 21 days. High, middle, low dose groups were given upper middle lower generic gout decoction 5, 10, 20 g·kg-1continuously for 21 days, ig respectively. The concentration of uric acid (UA), xanthine oxidase (XOD), adenosine deaminase (ADA), blood urea nitrogen (BUN)and creatinine (Cre) were detected in serum. Result: The content of UA of high, middle, low dose groups were (188.99±20.82), (202.56±19.77), (226.53±29.83) μmol·L-1respectively, which was significantly lower than those of the model group (310.12±22.23) μ mol·L-1,(P<0.05), also distinctly lower than that of allopurinol group (P<0.05). The XOD content of middle group was (40.15±0.74) U·L-1, which was significantly lower than that in the model group (49.19±1.61) U·L-1, (P<0.05). ADA content of high and middle dose groups (24.02±0.32), (28.61±1.78) U·L-1, respectively were obviously lower than those in the model group (34.36±3.73) U·L-1,(P<0.05). Both BUN and Cre content of all the three dosage groups were significantly lower than those in the model group(P<0.05). Conclusion: Upper middle lower generic gout decoction show better anti-hyperuricemia effect,and also significantly reduce the BUN level and downregulate the production of UA, meanwhile, it exerts protective effects on the kidney.关键词：Upper Middle Lower Generic Gout Decoction;hyperuricemia;uric acid;kidney
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发布时间：2024-01-04 - 摘要：Objective: To investigate hormonal profile and metabolic abnormalities in subgroups of women with polycystic ovary syndrome(PCOS) and explore a reasonable classification for PCOS. Method: Two hundred and thrity-five PCOS patients were recruited and divided into subgroup according to their body mass index(BMI)and homeostasis model assessment (HOMA-IR), serum sexual hormone,insulin,sugar metabolism,serum lipid levels were analyzed. Result: ① The level of testosterone(T),fasting plasma glucose(FPG), fasting insulin (FINS), 2 hour fasting insulin(2 h-INS),triglyceride (TG),low density lipoprotein cholesterol (LDL)of the obese PCOS group were higher than those of the Non-obese PCOS group, while the level of follicle stimulating (FSH),luteinizing hormone(LH),high density lipoprotein cholesterol (HDL) were lower than the Non-obese PCOS group, all the differences were significant (P<0.05);② body mass index(BMI),fasting plasma glucose(FPG),fasting insulin(FINS),2 h-plasma glucose (2 h-PG),2 h-INS,TG,HDL were higher than that of the group without insulin resistance,luteinizing hormone(LH),high density lipoprotein cholesterol (HDL) were lower than that of the group without insulin resistance,all the differences were significant (P<0.05). Conclusion: ① The non-obese PCOS patients were characterized by hypothalamus-pituitary gland-ovary (HPO) axis dysfunction, the obese PCOS patients are easier to present insulin resistance;② there is a metabolic disturbance in patients with PCOS,which is more obvious dyslipidernia in obese and insulin-resistant patients.关键词：polycystic ovary syndrome;insulin resistance;body mass index
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发布时间：2024-01-04 - 摘要：Objective: To investigate the effect of herbal decoction with prevention of adhesion on recovery of the patients after laparoscopic endometriosis. Method: Eighty-six patients were randomly divided into two groups. The patients in the control group underwent the conventional treatment after surgery;other patients in the treatment group were given the herbal decoction 6 hour after surgery besides the conventional treatment.The patient's postoperative recovery time of defecation,gas exhaust,borborygmus recovery, the changes of the of temperature,white blood cell(WBC),interleukin-1β(IL-1β) and IL-6 were observed. Result: The patients in the treatment group were significantly better than those in the control group in borborygmus recovery time, the first time of gsa exhaust and defecation. The differences of the change of the first day's temperature and the first and the third day's WBC values were statistically significant in two groups. The levels of IL-1β and IL-6 in the treatment group were significantly lower compared with the control group after 72 h. Conclusion: The herbal decoction can accelerate the early resumption of gastrointestinal function, shows broad-spectrum antibacterial ability and thus can diminish inflammation and reduce the postoperative complications.关键词：Fangzhanlian tang;laparoscopic endometriosis;IL-1β;IL-6;peritoneal adhesion
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect of herbal prescription with Bushen Huayu fang on endocrine environment in polycystic ovarian syndrome(PCOS) patients with insulin resistance (IR) and kidney deficiency and blood stasis syndrome. Method: Thirty-six patients were given herbal prescription with tonifying kidney and removing blood stasis with one treatment course of 3 months.3 months later the internal secretion sex hormone value, body weight index (BMI), the blood sugar and the insulin level were detected. Result: Compared with before treatment, the level of luteum hormone (LH), BMI and insulin of take sugar 2 h significantly reduced after treatment, and there were statistically significant difference(P<0.05). Conclusion: The herbal prescription with Bushen Huayu fang can adjust endocrine disorder status and improve insulin resistance in PCOS patients with insulin resistance and kidney deficiency and blood stasis syndrome.关键词：polycystic ovarian syndrome;insulin resistance;Bushen Huayu fang;endocrine environment
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发布时间：2024-01-04 - 摘要：Objective: To analysis the effect of Mailuoning injection in the treatment of different traditional Chinese medicine(TCM) syndrome types of acute ischemic cerebrovascular disease. Method: Neural department of internal medicine from July to 2012 in our hospital in 2011 July from hospitalized patients with acute ischemic cerebral vascular disease in 78 cases as the object of study, according to the patient's condition of TCM syndrome types of all patients were divided into four categories, were given routine treatment of Western medicine combined with Mailuoning injection, compared the clinical efficacy, activity and scores of quality of life in patients with difference after treatment. Result: The phlegm-heat accumulation, wind phlegm treatment of disturbance of patients after treatment of the total efficiency was significantly lower than the other types (P<0.05); phlegm-heat accumulation, and the quality of life of active scoring windphlegm type was significantly lower than the other types (P<0.05). Conclusion: Mailuoning injection can be effective in the treatment of acute ischemic cerebral vascular disease, but the effect of different TCM syndrome types of not one, for phlegm-heat accumulation effect, windphlegm type than other types of difference.关键词：Mailuoning injection;acute ischemic cerebrovascular disease;curative effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the clinical efficiency of the combined traditional Chinese medicine(TCM) therapy method in treatment of acute pancreatitis based on the differentiation of syndrome. Method: The acute pancreatitis were divided into acute exacerbation, acute convalescence, mild acute pancreatitis and severe acute pancreatitis.The differentiation of syndrome of TCM was divided into the acute exacerbation into wetness-heat of liver and gallbladder syndrome type, stomach-intestine excessive heat type and vital Qi desertion due to interior invaded by pathogen type. The acute convalescence was divided into liver depression and spleen deficiency type and Qi depression to blood stasis type. The TCM quadric-combination therapy was used in the ways of gavages, enema, packet, intravenous drip. This therapy was divided into different levels, different stages and different types according to differentiation of syndrome. Result: The time of disappearance of abdominal pain(5.1±1.05)d, the time of disappearance of abdominal distension(4.01±1.35)d, the gurgling sound resume time(1.97±0.21)d, the average hospitalization time(16.65±1.86)d, the recovery time of normal blood amylase(3.02±0.52)d, the recovery time of normal lipase:(4.64±0.61)d, the recovery time of normal C-reaction protein(CRP) (6.15±0.63)d, the recovery time of normal white blood cell(WBC)(4.24±0.36)d. There is one complication of cardiac insufficiency happened during the therapy. Conclusion: The optimum proposal can improve clinical effects, decrease complications, shorten the hospitalization and reduce the expenses of medicines.关键词：acute pancreatitis;four kinds of traditional Chinese medicine combination therapy method;differentiation of syndrome;clinical therapeutic effect
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发布时间：2024-01-04 - 摘要：Objective: To observe the effect on treating diabetic foot with tetramethylpyrazine(TMP) combined with foot bath. Method: Ninty patients were randomly divided into A(control group),B(TMP treated group),C(TMP combined with foot bath treated group), 30 cases in each group were given conventional treatment. On this basis, group B was treated with TMP. On the basis of the group B, group C was treated with traditional Chinese medicine foot bath accordance with the principles of the syndrome differentiation. 4 weeks later. Effect of the treatment of patients,the improvement of nerve conduction velocity (MCV) and sensory nerve conduction velocity (SCV) in each group were compared. Result: compared with group A, the treatment of patients with total efficiency in groupB and C were significantly improved and the difference was statistically significant (P<0.05). The treatment of patients with total efficiency in group C was significantly improved than that of in group B, and the difference was statistically significant (P<0.05); compared with group A, the SCV in groupB and C were significantly improved and the difference was statistically significant (P<0.05), The SCV in group C was significantly improved than that of in group, and the difference was statistically significant (P<0.05). Conclusion: On the basis of conventional Western medicine treatment, TMP combined traditional Chinese medicine foot bath treatment of diabetic foot can significantly improve the treatment efficiency and SCV,which can Promote lesion recovery of neurological function, and it is worthy of clinical application.关键词：tetramethylpy razine;foot bath;diabetic foot;nerve conduction velocity
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect and mechanism of Zhishi Xiebai Guizhi decoction on matris metalloproteinases-9(MMP-9), tissue inhibitor of metalloproteinase(TIMP-1) levels and gene expression in patients with blood stasis,sputum,qi stagnation,cold obstruction unstable angina (UA). Method: Sixty UA patients were divided into control group(30)and treatment group(30) randomly. ELISA was used to determine the serum MMP-9, TIMP-1 levels. Real time fluorescent quantitative PCR was used to determine MMP-9,TIMP-1 gene expression. Compared the serum MMP-9, TIMP-1 levels and gene expression from the two group. Result: The two groups had significant improvements in Symptom integra after treatment(P<0.05), and the treatment group were superior to the control group(P<0.05).The two groups had significant improvements in Serum levels of MMP-9, TIMP-1 after treatment(P<0.05).and the treatment group were superior to the control group(P<0.05). The two groups had significant improvements in MMP-9,TIMP-1 mRAN after treatment(P<0.05). The treatment group were superior to the control group in MMP-9/TIMP-1mRAN(P<0.05). Conclusion: Based on the standardization of treatment, Zhishi Xiebai Guizhi decoction can improve the serum MMP-9, TIMP-1 levels and gene expression in patients with blood stasis,sputum,qi stagnation,cold obstruction UA, and advance the stability of atherosclerosis plaque.关键词：Zhishi Xiebai Guizhi decoction;unstable angina;MMP-9;TIMP-1
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发布时间：2024-01-04 - 摘要：Objective: To observe the therapeutic effect of Sini tang and retention enema in the treatment of infantile diarrhea. Method: One hundred and eighty infantile diarrhea patients were randomly divided into treatment group(90 cases) and control group(90 cases) by the ratio of 1:1,the treatment group was given Sini tang decoction enema,while the control group was orally given the combined Bacillus Subtilis and Enterococcus Faecium Granules(Medilac-Vita). After 72 hours,clinical efficacy and adverse reactions were evaluated and observed. Result: The total effective rate in the treatment group was 83.34%, which was higher than that that in the control group (72.22%) (P<0.05). Conclusion: Sini tang decoction enema has a obvious effect in infantile diarrhea and no obvious adverse reaction. The method of retention enema in the treatment of infantile diarrhea with no adverse reactions.关键词：infantile diarrhea;Sini tang;retention enema;external treatment for internal diseases
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发布时间：2024-01-04 - 摘要：Objective: To explore the effect of Yiqi Huayu Lishui decoction (YQHYLSD) on vascular endothelial growth factor (VEGF) and urinary albumin excretion rate (UAER) in patents of early diabetic nephropathy combined with HIV/AIDS. Method: Fifty six cases of early diabetic nephropathy combined with HIV/AIDS were randomly divided into two groups. Two groups were received routine western medicine treatment and antivirus. The treatment group was treated with YQHYLSD one dose daily for 3 months. The levels of plasma VEGF in the two groups were measured by immunohistochemisty and enzyme linked immunosorbent assay. The urinary albumin excretion was measured to calculate UAER. Result: The plasma levels of VEGF and UAER in treatment group were significantly lower then those of control group, and the outcome in treatment group was more effective then that in the control group(P<0.05). Conclusion: VECG and UAER play an important role in the pathogenesis of early diabetic nephropathy patients combined with HIV/AIDS. YQHYLSD can degrade VEGF and UAER levels of early diabetic nephropathy patients, which is one of possible mechanism of YQHYLSD protecting the renal function.关键词：Yiqi Huayu Lishui decoction;diabetes nephrology combined with HIV/AIDS;VEGF;UAER
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发布时间：2024-01-04 - 摘要：Objective: To observed the efficacy of Wenzhong Zhitong prescription in treating functional abdominal pain (FAP) in children. Method: Ninety-two Cases of FAP in children were randomly divided into treatment group (58 cases) and control group (34 cases). Patients in treatment group were given Wenzhong Zhitong prescription, and those in control group were given Anisodamine (654-2). The clinical curative effects, main symptoms and signs were recorded for comparative analysis before and after 2 weeks-treatment. Result: The total effective rate of treatment group (94.83%) was obviously higher than that of the control group (82.35%), there were significant difference between the two groups (P<0.05). Compared to the control group, the treatment group showed better effects, as respect to the alleviation in symptoms of abdominal pain, reduction in frequency of abdominal pain, and relief of abdominal pain. The reoccurrence rate in the treatment group is obviously lower than that of the control group, and there were significant difference between them(P<0.01). Conclusion: Wenzhong analgesic prescription in treating FAP in children is safe and effective, and should be widely applied.关键词：functional abdominal pain;TCM therapy;Wenzhong Zhitong Prescription;children
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发布时间：2024-01-04 - 摘要：Objective: Through the systematic collection of ancient and modern drugs in treatment of ulcerative colitis to summarize the ancient and modern used effective drugs and analysis of medicine laws including compare drug trends between ancient and modern medicine. Method: The ancient and modern prescriptions were collected and SPSS 11.5 statistics software was applied to establish a database, so as to summarize medicine laws depending on correlation analysis, frequency analysis and cluster analysis as well as difference between ancient and modern medicine made by student t test. Result: A total of 461 the ancient prescription and 286 the modern prescription collected. Licorice, angelica, ginger, wood, poria et al of ancient high-frequency medicine and licorice, woody, poria, dried tangerine peel, Magnolia et al of modern high-frequency were showed by frequency analysis. The significant positive correlation between the medicine were showed by correlation analysis. UC prescription medicine compatibility relations were further pointed out by cluster analysis. A significant difference between ancient and modern UC drug trends was showed by student t tests. Conclusion: The ancient prescriptions are applied to supplement Qi and blood, invigorate spleen and stomach deduced by the statistical data. While, the modern prescriptions are applied to replenish lung and spleen Qi, clear heat and remove dampness detoxification, promote blood circulation and remove blood stasis, harmony Qi and purge stagnation, spleen and stomach, invigorate spleen and stomach. There was significant difference between ancient and modern medicine trends.关键词：ulcerative colitis;statistical analysis of the drugs;analysis and comparison
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发布时间：2024-01-04 - 摘要：Objective: To evaluate the safety and efficacy of compound Kushen injection (CKI) as adjunctive treatment of patients with advanced gastric cancer (AGC). Method: A computer-based online search of literatures prior to March 2012 was performed in Medline, the Cochrane Library, EMBASE, CENTRAL, PubMed, Google Scholar, CNKI, CBM, VIP and Wanfang by using a combination of synonyms for compound kushen injection, gastric cancer and chemotherapy. Randomized controlled trials(RCT)of CKI as adjunctive treatment for AGC were included. Bias risks of included RCT studies were evaluated and homogeneity studies were Meta-analyzed by Revman 5.1 software. Result: Twelve RCT studies including 1 004 patients were involved in this research. Pooling data of the Meta-analysis indicated that compared with chemotherapy alone, the combination had a statistically significant benefit in total effect rate [OR=1.51,95%CI (1.18, 1.94), P=0.001], quality of life improvement rate [OR=1.95, 95%CI (1.49,2.55), P<0.000 01] and improved the incidence of Leukopenia [OR=0.36, 95%CI (0.26, 0.50), P<0.000 01]. Conclusion: The results of this Meta-analysis confirmed that CKI plus chemotherapy shows a good efficacy and an acceptable safety profile in AGC patients, and may prove to be worthy of widespread use.关键词：compound Kushen injection;gastric cancer;chemotherapy;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To study the efficacy and safety of Shenfu injection(SFI) in combination with platinum-contained first-line chemotherapy in treating non-small cell lung cancer (NSCLC). Method: Cochrane library,PubMed,CBM,CNKI,VIP,WANFANG DATA and Traditional Chinese Medicine Database System were searched through computers. The search was finished in September,2012.Randomized controlled trials (RCTs) of SFI in combination with platinum-contained first-line chemotherapy (versus chemotherapy alone) in any language were included. Two researchers extracted data and assess literature quality separately,and made a meta-analyses by RevMan 5.1 software. Result: A Total of 19 RCTs was included. The Meta-analysis showed that compared with the pure application of platinum-contained first-line chemotherapy,the combination of SFI and chemotherapy can not enhance the near-term curative effect [OR=1.41,95%CI(0.98,2.02),P=0.06], but may improve the quality of life [OR=2.72,95% CI(1.48,5.00),P=0.001], enhance CD3+[SMD=0.41,95% CI(0.10,0.71),P=0.008], CD4+[SMD=7.58,95% CI(4.32,10.84),P<0.000 01] and CD4+/CD8+[SMD=9.89,95% CI(4.76,15.02),P=0.000 2] and reduced WBC toxicity [RR=0.35,95% CI(0.23,0.54),P<0.000 01],Hemoglobin toxicity(RR=0.41,95% CI:0.20~0.83,P=0.01),Platelet toxicity [RR=0.34,95% CI(0.18,0.65),P=0.001],the gastrointestinal reactions [RR=0.24,95% CI(0.13,0.45),P<0.000 01)]. Conclusion: The current evidence indicates that SFI can not enhance the chemotherapeutic effect on NSCLC patients,but may improve the quality of life and reduce adverse effect of contained therapy of platinum and chemotherapeutics. These results require confirmation with rigorously controlled trials.关键词：Shenfu Injection;non-small cell lung cancer;chemotherapy;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective: To analyze Professor YANG Wen-hua's herbal administration experience for Leukemia treatment based on traditional Chinese medicine inheritance support system(V1.1). Method: Collecting the prescriptions used for Leukemia patients treated with chemotherapy, data were logged into the traditional Chinese medicine inheritance support system,by using principle analysis,revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering, together with Professor YANG's personal proofreading to analyze the composing principles of prescriptions——-the frequency of drugs,coupled drugs and composing characters. Result: One hundred and fifty-three prescriptions were bolted and analyzed, in which 142 kinds of herbs appeared, after checking the frequency of each herb and the relative rules between all kinds of drugs, we got 18 core groups and 8 new prescriptions, and these were conformed as the core herbs for treating Leukemiatreated with chemotherapy by Professor YANG Wenhua herself. Conclusion: Professor YANG Wenhua's prescriptions has outstanding characteristics in treating Leukemia and accurate. The consequences were obtained by the traditional Chinese medicine inheritance support system and the professor's experience is generally accordant, the system can be used to analyze and summarize the experiences of famous veteran doctors of Traditional Chinese Medicine.关键词：YANG Wenhua;leukemia in the chmotherapy period;herbal administration experience;traditional Chinese medicine inheritance support system(V1.1)
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发布时间：2024-01-04 - 摘要：Objective: Based on the association rule, to find the relationship between the traditional Chinese medicine(TCM) nature and other properties. Method: Many text book were used as the information source,such as ‘Chinese pharmacopoeia’, ‘Chinese materia medica’,etc. And combined with the empirical clinical medication,we builded the Chinese medicine database. Based on the improved Apriori algorithm, we analyzed the relationship between the TCM nature and other properties. Result: We have found the closely frequent itemsets in Nature-Pharmacology, Nature-Indication, Nature-Western medicine name, Nature-Function, Nature-Flavour, Nature-Channel tropism, Nature-Chemical composition,etc. And we calculated support and support rate in the TCM nature and other properties. Conclusion: Through analyzing the relationship between the TCM nature and other properties based on the association rule, the analyzability of results are reasonable which is consistent with the traditional understanding of the TCM nature.关键词：association rule;nature of traditional Chinese medicine;relationship of properties;frequent itemsets;data mining
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发布时间：2024-01-04 - 摘要：To analyze effective methods for improving and enhancing the bioavailability of the active ingredients in the traditional Chinese medicine (TCM). On the basis of summarizing and analyzing the biopharmaceutical and pharmacokinetic characteristics of osthol, the main active ingredient of fructus cnidii, pharmaceutical methods and components compatibility are proposed to have influence on the bioavailability and disposition of osthol. Osthol, as a poorly water soluble drug, had a good permeability, but bioavailability was poor. Pharmaceutical methods and components compatibility are helpful to improve the poor bioavailability of osthol. To improve and enhance the bioavailability of the active ingredients in the TCM, we can not only resort to pharmaceutical methods, but also the reasonable application of components compatibility between the TCM.关键词：osthol;diposition;bioavailability;components compatibility;pharmaceutical method
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发布时间：2024-01-04 - 摘要：This article reviews the application of tandem mass spectrometry reported in recent years in the chemical components analysis of traditional Chinese medicines (TCMs). To analysis the application of tandem mass spectrometry in the chemical composition of alkaloids, flavonoids, steroids and triterpenoids, coumarin and lignans, phenolic acids and other single and prescription of TCMs. Result displays tandem mass spectrometry has developed into an important means for in vivo and in vitro of TCMs analysis due to its ability of powerful structure, that play an increasingly important role for material basis and mechanism of action of TCMs.关键词：tandem mass spectrometry;pharmaceutical chemistry;component analysis
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发布时间：2024-01-04
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