最新刊期
卷 19 ， 期 13 ， 2013
- 摘要：Objective:To evaluate uncertainty of total error from instrument indicated value in pH determination of Chinese herbals water extract. Method: Primary pH standard substance was used and standard solution measurement was selected for evaluation. Result: Main factors affecting uncertainty of total error for pH meter’s indicated value were measuring repeatability,standard solution and the liquid junction potential,and the assessed uncertainty U95was 0.012pH. Conclusion: Evaluation method for uncertainty introduced in this article was accurate and reliable,it could provide scientific basic data for study on valuation and uncertainty evaluation of pH value of Chinese herbals water extract.关键词：water extract of Chinese materia medica;pH meter;uncertainty
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发布时间：2024-01-04 - 摘要：Objective:To prepare resveratrol hydroxypropyl-β-cyclodextrin(HP-β-CD)inclusion compound and investigate effect of 4 kinds of penetration enhancers on percutaneous absorption of this inclusion cream. Method: Resveratrol HP-β-CD inclusion compound was prepared with lyophilization technique,entrapment efficiency of this inclusion compound was investigated by HPLC.Modified Franz diffusion cell was applied to investigate in vitro transdermal absorption behavior of resveratrol HP-β-CD inclusion compound cream with isolated mouse skin as barrier and chose the best penetration enhancer. Result: Prepared resveratrol HP-β-CD inclusion compound was in high entrapment efficiency.There was large difference when different transdermal enhancers or different concentrations of enhancers were used,1% azone was optimum penetration enhancers. Conclusion: Resveratrol HP-β-CD inclusion compound had high stability,prepared cream with 1% azone as penetration enhancers had the best transdermal permeation behavior,resveratrol HP-β-CD inclusion compound cream had a great development prospect in the beauty.关键词：resveratrol;inclusion compound;transdermal absorption;penetration enhancer;cream
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发布时间：2024-01-04 - 摘要：Objective:To investigate effect of different receiving solution on in vitro drug transdermal test. Method: 5-fluorouracil(5-FU) was applied as model drug,receiving solutions of a certain concentration of bovine serum albumin(BSA),Brij98,poloxamer188,tween80,ethanol,γ-cyclodextrin and sodium dodecyl sulfate(SDS) fro transdermal test were prepared by physiological saline,the excised rat skins were treated with various additives for 12 h,followed by replacing those receiving solution with normal saline and adding saturated 5-FU into the donor cells to evaluate transdermal absorption of the model drug.Meanwhile,scanning electron microscope(SEM) was used to investigate morphology of the rat skins treated with those receiving solutions. Result: 3%BSA,6%Brij98 and poloxamer188,ethanol,tween80 and γ-CD with low concentration had no significant effect on in vitro drug transdermal absorption parameters,but they would exert an influence on the rat skins when the concentration of those four additives were high.In addition,ionic surfactant of SDS had an severely impact on the excised rat skins. Conclusion: Those additives,3%BSA,6%Brij98,the low concentration of tween80,poloxamer188,ethanol and γ-CD could be added to receiving solution in order to increase drug solubility in proper concentration.But ionic surfactant should be cautious to use as additives of receiving solution.关键词：receiving solution;additives of receiving solution;in vitro transdermal test;percutaneous penetration
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology conditions of deacidification evening primrose oil by supercritical CO2 fluid extraction-fractionation technology(SCFEF). Method: With yield of oil and the content of γ-linolenic acid as indexes,single factor tests were adopted to investigate effect of extraction pressure,extraction temperature,separation pressure and separation temperature on extraction technology of evening primrose oil.And quality differences of evening primrose oil was compared,which were extracted by SCFEF and supercritical CO2 fluid extraction(SCFE). Result: Optimal extraction parameters were as follows:extraction pressure 22 MPa,extraction temperature 36 ℃,separation pressure 10-12 MPa,separation temperature 48 ℃,dosage of CO2 45 kg,oil yield 16.7%-18.1%,the acid values of 0.85-1.21 mg KOH·g-1,each physical and chemical indicators of evening primrose oil from SCFEF were better than that from SCFE. Conclusion: SCFEF successfully prepared deacidified evening primrose oil by integration of extraction,separation and purification processions into one step.关键词：supercritical CO2fluid extraction-fractionation technology;evening primrose oil;deacidification;γ-linolenic acid
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发布时间：2024-01-04 - 摘要：Objective:To optimize particle size of ultrafine powder by investigating in vitro dissolution and receding jaundice effect of micronized Canhuang tablets(MCHP). Method: With berberine as index,release of berberine from MCHP with different crush size in different time periods was determined by HPLC,calculated cumulative dissolution,and then observed dissolution.At the same time,rats jaundice model was caused by 4%ANTI,after administered in accordance with dosing regimen,the femoral artery blood was collected for determining serum biochemical markers,such as ALT,AST,TBIL,DBIL and γ-GT,receding jaundice effect of MCHP with different grinding particle size was evaluated.Superfine powder particle size of MCHP was optimized by Comprehensive evaluating in vitro dissolution and receding jaundice effect. Result: With increasing of pulverized mesh,cumulative dissolution of berberine increased,cumulative dissolution of 200,300 mesh was 94.86%,96.18% at 60 min,respectively.For receding jaundice effect,Canhuang tablets with 200 mesh could significantly reduce ALT,AST and γ-GT,which was stronger than those of Canhuang tablets with 100,150 and 300 mesh,furthermore,that of 200 mesh was superior to control drug ursodeoxycholic acid tablets in reducing ALT and γ-GT.Comprehensive appearance of tablets and actual production,200 mesh was determined as optimum crush size. Conclusion: It was a feasible and convenient method to optimize ultrafine powder particle size of MCHP by combining in vitro dissolution and receding jaundice effect.关键词：Canhuang tablets;ultrafine grinding;dissolution;receding jaundice
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发布时间：2024-01-04 - 摘要：Objective: To prepare sustained release pellets of total tanshitones and optimize its formulation technology. Method: Drug-loaded pellets core was prepared by solid dispersion solubilization technology and fluidized bed technology,this pellets core was coated with Eudragit NE30D,single factor test was used to investigate effect of coating weight,type and dosage of porogen,anti-adhesive agent,curing time and temperature on coating technology. Result: Optimum coating technology was as following:coating weight of Eudragit NE30 5%,with 20% lactose as porogen,50% talcum powder as anti-adhesive agent,curing time 12 h,curing temperature 40 ℃.Coated pellets had good sustained release effect,in vitro release of this drug was in line with First-order release equation. Conclusion: Fluidized bed irrigation method and fluidized bed coating method could be adopted to prepare sustained release pellets of total tanshinones from Salvia miltiorrhiza with slow and stable in vitro release.关键词：total tanshintones;sustained release pellets;EudragitNE30D;porogen
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of Huayu Qutan granules by central composite design-response surface methodology. Method: Effective parts of Huayu Qutan granules was extracted by decoction,with the content of salvianolic acid B as index,extraction times,ratio of solid to liquid and extraction time as independent variables,nonlinear mathematic models were used to estimate the relationship between independent and dependent variables,response surface analysis were delineated according to these mathematic models,and optimum technology conditions were selected. Result: Optimum extraction technology was as following:extracted 3 times with 10 times the amount of water,1.5 h each time.RSD between the measured value(1.027%) and the theoretical value(1.029%) of the content of salvianolic acid B was small. Conclusion: Central composite design-response surface methodology was successfully used to optimize extraction technology of compounds,optimized technology was stable and feasible,established mathematical model had good predictability.关键词：central composite design;response surface methodology;salvianolic acid B
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发布时间：2024-01-04 - 摘要：Objective:To optimize molding technology with vacuum freeze drying of Huoxue Tongluo powder for injection,and investigate its stability. Method: With composite score of freeze-dried molding effect,solubility,clarity,the content of ferulic acid and paeoniflorin as index,based on single factor test,effect of dosage of mannitol,liquid concentration,pre-freezing rate on freeze drying technology was investigated by orthogonal design,the content of ferulic acid and paeoniflorin was determined by HPLC. Result: Optimized molding technology was as following:with 5%mannitol as filler,liquid concentration 30 g·L-1,prefreezing rate 30 ℃·h-1,these prepared freeze-dried powder injection had stable quality. Conclusion: Optimized prescription and freeze-drying process was reliable and feasible,it could be used in large-scale production.关键词：Huoxue Tongluo powder injection;freeze drying;eutectic point temperature;freeze drying curve
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction process of Xifeng Tongnao capsules. Method: With the content of paeoniflorin and yield of extract as indexes,the amount of water,soaking time,extraction time and extraction times were chosen as factors,orthogonal design was used to optimize water extraction process of Radix Paeoniae Rubra and nine other Chinese herbal pieces from Xifeng Tongnao capsules;With yield of extract as index,the amount and concentration of ethanol,soaking time and reflux extraction time were selected as factors,orthogonal design was used to optimize alcohol extraction process of Radix Et Rhizoma Notoginseng from Xifeng Tongnao capsules. Result: Optimum water extraction technology was as following:soaked 0.5 h with 10 times the amount of water,extracted 2 times, 1.5 hours per time;Optimum alcohol extraction process was as following:soaked 0.5 h with 6-fold of 70% ethanol,reflux extracted 3 times with extraction time of 1.5,1,1 h. Conclusion: This optimized extraction technology was stable and reliable,it could be extended to large-scale production application of Xifeng Tongnao capsules.关键词：Xifeng Tongnao capsules;orthogonal test;extraction technology;paeoniflorin
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction process of Kangshiming mixtures. Method: With the content of puerarin and paeoniflorin as indexes,which were determined by HPLC,Orthogonal test was applied to optimizing extraction process with the amount of water,extraction time and times as factors. Result: The best extraction conditions were as follows:extracted 3 times with 10-fold the amount of water,1 h each time. Conclusion: This optimized extraction process was reasonable and feasible,it could be extended to industrial production applications of Kangshiming mixtures.关键词：Kangshiming mixtures;orthogonal design;puerarin;peoniflorin
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发布时间：2024-01-04 - 摘要：Objective:To optimize purification process of total flavonoids from Baeckea frutescens L. by macroporous adsorption resin. Method: Different types of resin were selected with absorption and desorption ratio of total flavonoids as indexes,the content of total flavonoids was determined by UV,single factor test was adopted to investigate purification technology of total flavonoids by macroporous adsorption resin. Result: AB-8 type resin was adopted,its optimum process conditions were as followings:the concentration of sample solution 0.3 g·mL-1,absorption velocity 1 BV·h-1,sample volume 3 BV,eluted by 5 BV 50% ethanol at flow rate of 2 BV·h-1.Under these conditions,purity of total flavonoids was up to 52.33%. Conclusion: Optimized process was rational,feasible and suitable for industrial production.关键词：Baeckea frutescens L.;total flavonoids;macroporous adsorption resin;purification
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发布时间：2024-01-04 - 摘要：Objective:To optimize extraction technology of compound preparation of Periploca forrestil. Method: Orthogonal design was used to investigate effect of solid-liquid ratio,ethanol concentration,extraction time and times on extraction technology of compound preparation of P. forrestil with composite score of total alkaloids yield and dry extract rate as index. Result: The best extraction conditions were as followings:extracted 3 times with 6-fold 70% ethanol,1 hour for each time. Conclusion: This optimized extraction technology was stable and feasible for preparing new formulation of extraction technology of compound P. forrestil.关键词：compound Periploca forrestil;orthogonal design;extraction process;total alkaloids
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发布时间：2024-01-04 - 摘要：Objective:To investigate separation effect of iridoid glycosides from Cornus officinalis by different types of macroporous resin. Method: The content of loganin and morroniside from C. officinalis was determined by HPLC,effectiveness of different macroporous adsorption resin on separating iridoid glycosides from C. officinalis was investigated. Result: HPD-400 type of macroporous resin had the best separation effect.Regression equation of loganin and morroniside were Y=17.245X+13.362(r=0.999 9),Y=15.468X-41.275(r=1.000 0);The linear range of them were 21.75-435,35.8-716 μg;Recoveries were 98.27%,96.29%,respectively. Conclusion: It was feasible that selected the content of loganin and morroniside as index to screen macroporous resin for enrichment iridoid glycosides from C. officinalis,this study could provide experimental basis for the follow-up study of separation of effective parts from C. officinalis.关键词：Cornus officinalis;macroporous resin;iridoid glycosides;loganin;morroniside
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发布时间：2024-01-04 - 摘要：Objective:To prepare Xiaoer Fuxie topical gel ointments,and establish its quality control standards. Method: Gel ointments was prepared with extract of Fructus Evodiae,Fructus Piperis,Flos Caryophylli and Cortex Cinnamomi as materials,sodium polyacrylate and carbomer as gel skeleton matrix.Fructus Piperis,Flos Caryophylli and Cortex Cinnamomi were identified by TLC.The content of evodiamine was determined by HPLC. Result: Moldability of prepared gel ointments was good with a suitable mechanical strength and adhesion.Fructus Piperis,Flos Caryophylli and Cortex Cinnamomi were detected by TLC,characteristic identification was distinct and highly specific.Evodiamine showed a good linear relationship in the range of 0.05~0.4 μg (r=0.999 9) with an average recovery of 99.3% (RSD 1.01%). Conclusion: This preparation technology was stable and reasonable.This established quality control method was reliable,accurate and specific,which could be used for quality evaluation of Xiaoer Fuxie topical gel ointments.关键词：Xiaoer Fuxie topical gel ointments;quality standards;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To investigate extraction rate variation of harpagide in different separated recipes of Simiao Yong'an decoction,and explore its regularity of compatibility. Method: Extraction rate of harpagide from 8 separated recipes of Simiao Yong'an decoction was determined by HPLC,using a SunFireTM C18 column (4.6 mm×150 mm,5 μm),acetonitrile-water containing 0.1% H3PO4 in gradient elution as mobile phase,the detection wavelength was set at 250 nm,the flow rate was 1.0 mL·min-1,column temperature of 35 ℃,then extraction rate variation of harpagide was analyzed. Result: The regression equation of harpagide was Y=1.22×106X+3 218.75(r=0.999 8) with the liner range of 0.065 3-0.457 μg,the average recovery was 96.81% with RSD of 1.83%. Extraction rate of harpagide from different separated recipes was in order of the original prescription>Scrophularia ningpoensis>other separated recipes. Conclusion: All these seven separated recipes of Simiao Yong'an decoction reduced extraction rate of harpagide,which showed a distinct advantage of the original prescription.And harpagide may be one of effective components in Simiao Yong'an decoction.关键词：Simiao Yong'an decoction;separated recipes;compatibility;harpagide;extraction rate
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC method for simultaneous determination of chlorogenic acid,jasminoidin and baicalin in Yinzhihuang dispersible tablets,and investigate in vitro dissolution characteristics of these three active components. Method: According to the 2010 edition of ‘Chinese Pharmacopoeia’,with distilled water was dissolution medium,the paddle method was used with rotate speed of 50 r·min-1,the content of these active components were determined by HPLC,the accumulative dissolution was calculated and the resemblance of dissolution curve was compared by similarity factors (f2). Result: Compared with chlorogenic acid,f2 of jasminoidin and baicalin were 89.23 and 48.35 in water,respectively;Compared with jasminoidin,f2 of baicalin was 46.98. Conclusion: Chlorogenic acid and jasminoidin from Yinzhihuang dispersible tablets had similar dissolution characteristics in distilled water,but baicalin was different from them.关键词：Yinzhihuang dispersible tablets;dissolution;similarity factor;chlorogenic acid;jasminoidin;baicalin
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发布时间：2024-01-04 - 摘要：Objective:To optimize preparation technology of curcumin by monascus liquid culture method. Method: With natural Curcuma longa as raw materials and yield of curcumin index,based on single-factor tests,effect of solid-liquid ratio,fermentation time,nutrition ratio and pH of fermentation broth on preparation technology was investigated by orthogonal test. Result: Optimum preparation technology was as following:solid-liquid ratio of 1 g·L-1,fermentation time 5 d,nutrition ratio 1/6,pH of fermentation broth 4.Yield of curcumin was up to 0.43%. Conclusion: This optimized technology was stable and feasible with high efficiency.关键词：monascus;curcumin;liquid culture;fermentation;significant
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发布时间：2024-01-04 - 摘要：Objective:To optimize β-cyclodextrin(β-CD) inclusion process of volatile oil from Xingqi Na'nihua granules. Method: β-CD inclusion complex was prepared by saturated aqueous solution method,inclusion process was optimized by uniform design with inclusion ratio of volatile oil and inclusion yield as indexes,taking ratio of volatile oil to β-CD,inclusion temperature and inclusion time as factors,and phase of this prepared inclusion complex was testified by XRD,DSC,IR,UV and TLC. Result: Optimum inclusion process was as follows:ratio of volatile oil to β-CD 1∶11,inclusion temperature at 60 ℃ and inclusion time 1 h.Inclusion ratio of volatile oil was as high as 96.84% under these optimized technology conditions.Validation result showed that inclusion compound had been formed. Conclusion: This optimized inclusion process was simple,stable and applicable to industrial production.关键词：Xingqi Na'nihua granules;volatile oil;β-cyclodextrin inclusion complex;uniform design test
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发布时间：2024-01-04 - 摘要：Objective:To establish a quality standard of Shegan Dilong Granules for scientific evaluation and effective control of its quality to provide a reliable basis. Method: TLC was used for identifying the herbs in the formula and the content determination of irisflorentin by HPLC was also revised. Result: The method was established to distinguish Belamcanda sinensis, pberetima, rhizome Menispermi and Schisandra chinensis. The spots in the TLC were clear, and with no interference from negative control. The HPLC separation was performed on C18 column, the mixture of acetonitrile-0.2% phosphoric acid water was used as mobile phase. The detection wavelength was set at 266 nm, irisflorentin was linear in the range of 0.342 2-86.60 mg·L-1;regression equation was A＝51 639C＋7 222 (R2＝0.999 5,n=10);the average recovery was 99.8%, RSD 1.50%. Conclusion: The method was simple and accurate with a good reproducibility, and can be used for the control of Shegan Dilong Granules.关键词：Shegan Dilong Granules;TLC;HPLC;quality standard;irisflorentin
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发布时间：2024-01-04 - 摘要：Objective:The high performance liquid chromatography (HPLC) method of precolumn derivatization with 1-phenyl-3-methyl-5-pyranozolone (PMP) was developed to efficiently separate several monosaccharides. And the monosaccharide composition and content of polysaccharides in the medicine pair of Dioscorea opposita and Rehmannia glutinosa and their single herb medicine were determined and compared. Method: A Hypersil ODS2 column with the mobile phase of 0.05 mol·L-1phosphate buffer (pH 6.7)-acetonitrile (83∶17) at the detection wavelength of 254 nm was used. The flow rate was 1 mL·min-1, the column temperature was kept at 30 ℃. Result: All three polysaccharides contained mannose, rhamnose, glucuronide acid, glucose, galactose and arabinose. However, fucose was not detected. At the same time, it was found that the content of mannose, rhamnose, glucuronide acid, glucose, galactose and arabinose in their medicine pair was between in the single Dioscorea opposita polysaccharides and Rehmannia glutinosa polysaccharides. Conclusion: The developed method is simple, rapid and accurate. Therefore, it is an efficient method to analyze the monosaccharide composition in different kinds of polysaccharides in drug and food.关键词：Dioscorea opposita polysaccharide;Rehmannia glutinosa polysaccharide;medicine pair polysaccharides;HPLC;precolumn derivatization
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发布时间：2024-01-04 - 摘要：Objective:To develop a method for content determination of total polysaccharide in Guizhitang decoction. Method: Glucose was used as the reference substance, and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide. Result: The calibration curve of glucose showed good linearity in the range of 19.62-117.70 mg·L-1. The average recovery rate was 99.28%, and RSD was 2.5% (n=6). Conclusion: The determination method is simple, sensitive and accurate, which can be used for evaluating the content of total polysaccharide in Guizhitang decoction.关键词：Guizhitang decoction;total polysaccharide;colorimetry;phenol-sulfuric acid;content determination
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发布时间：2024-01-04 - 摘要：Objective:To develop identification and assay methods of pinecone. Method: Pharmacognostic studies, extract mensuration, ash determination, moisture measuring method, and the bioactive constituents were analyzed by TLC and HPLC. In the HPLC method, isopimaric acid was separated on phenomenex Gemini-NX C18 110A column(4.6 mm×250 mm,5 μm) with methanol-2% acetic acid(90∶10) as the mobile phase with flow rate of 0.9 mL·min-1. The temperature of column was kept at 25 ℃. The detection wavelength was set at 210 nm. Result: The content of total ash, acid-insoluble ash, and moisture of 12 samples from different origins were determined. The qualitative and quantitative analysis of isopimaric acid by TLC and HPLC were preformed respectively. Quality specification of pinecone established through the experimental study:the moisture content less than 12%, total ash less than 1.3%, acid-insoluble ash less than 0.3%, extract not lower than 3.9%, isopimaric acid content not lower than 0.19%. Conclusion: The established method can be used for the quality specification of pinecone.关键词：pinecone;quality specification;isopimaric acid;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC-ELSD method for determination of monosaccharide content in different processed Radix Rehmannia Praeparata and to study the effect of different processed method on monosaccharide content in Radix Rehmannia Praeparata. Method: HPLC-ELSD was applied to analyze the content of monosaccharide with prevail carbohydrate ES column (4.6 mm×250 mm, 5 μm), acetonitrile-water (75∶25) as mobile phase. The flow rate was 0.8 mL·min-1;column temperature was kept at 30 ℃;temperature of drift tube set at 65 ℃, gas flow 2.5 L·min-1and gain 1. Result: The D-glucose and D-fructose average recovery was 98.24% (RSD 2.18%) and 98.37% (RSD 2.11%) respectively. The content of monosaccharide in Radix Rehmanniae from Wenzhi, steaming and stewing by liqour were 1.62%, 27.22%±2.74%, 9.63%±2.05%, 16.97%±2.62% respectively. Conclusion: The method is simple, accurate and reproducible. The content of monosaccharide in Wenzhi Dihuang was higher than that of steaming Dihuang and stewing by liqour. It provides an experimental basis to illustrate its processing mechanisms.关键词：HPLC-ELSD;Radix Rehmanniae Praeparata;Wenzhi;D-glucose;D-fructose
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发布时间：2024-01-04 - 摘要：Objective:To improve the quality standard of the traditional Mongolian patent medicine Sugmul-10 pills by the establishing of microscopic, TLC, physiochemical identification, and HPLC content assay methods. Method: Alpiniae oxyphyllae fructus, Caesalpinia fructus, Malva fructus and Eriocheir sinensis were identifred by microscopical analysis of episperm sclerenchymatous cells, scleroticnests and body wall debris in the sample powder. TLC identification of Alpiniae oxyphyllae fructus was performed on a silica gel plate GF254, with cyclohexane-ethyl acetate (9∶1) as developing solvent and standard medicinal materials as controls. Physiochemical methods were used to identify ammonium chloride in Sal Ammoniacus and calcium carbonate in Eriocheir sinensis. Piperine from Piperis longi fructus was selected for content supervision. HPLC method was performed on a ODS column, eluted with methol-H2O (77∶23), and detected at 343 nm. Result: Five medicinal materials from Sugmul-10 pills can be identified by the methods, including Alpiniae oxyphyllae fructus, Caesalpinia fructus, Malva fructus, Sal Ammoniacus and Eriocheir sinensis. HPLC content determination method showed good linearity(r=0.999 9) in the range of 0.040 4-0.282 8 μg. The average recovery rate of piperine was 98.1% with RSD 0.62%, and the content limitation is not less than 1.4 mg·g-1. Conclusion: This methods can effectively control the product quality.关键词：Sugmul-10 pill;Qualitative identification;determination of the content;quality control
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the determination of Panax notoginseng saponins and icariin in Guyu Injection. Method: Diamonsil C18 column (4.6 mm×250 mm, 3.5μm) was used with acetonitrile and water as mobile phase with gradient elution;the flow rate was 1 mL·min-1;detection wavelength was set at 203 nm;the column temperature was maintained at 25 ℃. Result: The linear range of icariin was within 1.06-10.60 μg and the average recovery of assay was 100.2% (RSD 1.54%);the linear range of notoginsenoside R1 was within 0.42-4.20 μg and the average recovery of assay was 100.6% (RSD 1.89%);the linear range of ginsenoside Rg1 was within 1.90-18.97 μg and the average recovery of assay was 99.9% (RSD 1.09%);the linear range of ginsenoside Re was within 0.18-1.78 μg and the average recovery of assay was 100.8% (RSD 1.96%);the linear range of ginsenoside Rb1 was within 2.23-22.34 μg and the average recovery of assay was 101.4% (RSD 1.83%);the linear range of ginsenoside Rd was within 0.45-4.53 μg and the average recovery of assay was 101.2% (RSD 2.37%). Conclusion: The method is accurate and reproducible. It can be used for determination of Panax notoginseng saponins and icariin in Guyu Injection.关键词：Guyu Injection;panax notoginseng saponins;icariin;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC method for simultaneous determination of gentiopicroside, loganic acid, swertiamarin and sweroside in Radix Gentianae. Method: Chromatographic separation was achieved on an Inertsil ODS-SP column (4.6 mm×150 mm, 5 μm) with methanol (A)-0.4% phosphoric acid (B) as mobile phase for gradient elution (0-40 min, 10%-30%A) at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 25 ℃ and the detecting wavelength was set at 240 nm. Result: The linear ranges were 1.0-20 μg (r=0.999 7) for gentiopicroside, 1.0-20 μg (r=0.999 6) for loganic acid, 1.0-20 μg (r=0.999 5) for swertiamarin, 0.1-2.0 μg (r=0.999 5) for sweroside. The average recoveries of four components were 99.1%, 99.0%, 98.9% and 98.5%, respectively. Conclusion: The developed method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of Radix Gentianae.关键词：Radix Gentianae;gentiopicroside;loganic acid;swertiamarin;sweroside;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for determination of hydrochloric berberine in Tianqidiedafengshi ointment. Method: Samples were analyzed on an Agilent TC-C18 column (4.6 mm×250 mm, 5 μm) using acetonitrile-0.05 mol·L-1 sodium dihydrogen phosphate(adjust the pH to 3.0 with phosphoric acid)(25∶75) as mobile phase. Speed of flow was 1.0 mL·min-1and examination wave length was 265 nm. Result: Hydrochloric berberine showed a good linearity in the range of 0.043 74-0.437 4 mg, egression equation was Y=0.000 2X+0.007 1 (r=0.999 9) and the average recovery was 99.34% (RSD 1.73%). Conclusion: The established methods are simple, accurate, reliable and can be used for the quality control of Tianqidiedafengshi ointment.关键词：Tianqidiedafengshi ointment;hydrochloric berberine;HPLC;qulity standard
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC method for the content determination of ZP-7 tenacigenin B in Marsdeniae Tenocissimae, and to determine the content of them in Marsdeniae Tenocissimae from different areas. Method: The analytical mothod was carried out with Agilent TC-C18(4.6 mm×150 mm, 5 μm) column and the mobile phase consisted of acetonitrile-water(35∶65)at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm, the column temperature was 25 ℃ and injection volume was 10 μL. Result: The calibration curves was linear within the range of 3.183-7.427 μg (r=0.999 4) for ZP-7 tenacigenin B. The average recovery was 99.95% (RSD 0.83%). Conclusion: The method is accurate, reproducible and precise, and can control the content of ZP-7 tenacigenin B in Marsdeniae Tenocissimae, and can guide the clinical medication.关键词：Marsdeniae Tenocissimae Caulis;ZP-7 tenacigenin B;HPLC;assay;anti tumor
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发布时间：2024-01-04 - 摘要：Objective:To clarify special constituents of purplish red ginseng, the kinds and content of ginsenosides in dry radix ginseng and purplish red ginseng were determined by UPLC-ESI-MS-MS method in this paper. Method: Waters ACQUITY UPLC BEH C18 (2.1 mm× 50 mm, 1.7 μm) column was used with mobile phase consisted of 0.1% methanoic acid - acetonitrile (gradient elution). The column temperature was set at 30 ℃. MS conditions:ESI, the negative electrospray ionization mode. Result: Compared with the dry radix ginseng, the kinds and content of ginsenosides in purplish red ginseng had changed, the content of 20(S)-ginsenoside Rg3, 20(R)-ginsenoside Rg3, 20(R)-ginsenoside Rh1, 20(R)-ginsenoside Rg2, Rk1, Rg5, Rd, Ro and zingibroside R1 increased in purplish red ginseng. Conclusion: Great changes of saponins have been obviously found in processing ginseng to purplish red ginseng. Special constituents of purplish red ginseng could be produced in ginseng processing.关键词：dry Radix Ginseng;purplish red ginseng;ginsenoside;UPLC-ESI-MS/MS
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发布时间：2024-01-04 - 摘要：Objective:To establish the method for quality control of Compound Quchongbanjiuju Pills. Method: Thin-layer chromatography and microscopic identification were used to identify Compound Quchongbanjiuju Pills. The content of pepper alkali was determined by HPLC. Result: Characteristic of microscopical identification was obvious. The TLC identification was highly specific and spots were clear. There was good linearity in the range of 5.42-43.36 mg·L-1of pepper alkali concentration (r=0.999 8), and the average recovery of pepper alkali was 100.72% with RSD of 0.56%. Conclusion: The method is simple and accurate, which can be applied to the quality control of the Compound Quchongbanjiuju Pills.关键词：Compound Quchongbanjiuju Pills;microscopical identification;TLC;HPLC;pepper alkali
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发布时间：2024-01-04 - 摘要：Objective:To develop high performance capillary electrophoresis (HPCE) fingerprints of Flos Farfarae and to provide the scientific basis for quality control of it. Method: Electrophoresis was performed on a 75 μm× 57 cm (effective length is 50 cm) fused silica capillary. 30 mmol·L-1 sodium borate contained 25% methanol adjusted to pH 8.8 was applied as the background electrolyte. The running voltage was 20 kV, the detection wavelength was at 214 nm and the temperature was kept at 25 ℃. Result: The HPCE fingerprint established from the 15 batches of the Flos Farfarae showed 26 characteristic common peaks, among which 6 peaks were recognized. Conclusion: The method is reliable, accurate, simple and quick. It provides a reliable method for the scientific quality evaluation of Flos Farfarae.关键词：Flos Farfarae;capillary electrophoresis;fingerprints
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发布时间：2024-01-04 - 摘要：Objective:To study chemical constituents of the fifth sort of new drugs-total terpene alcohols of Atractylodes lances. Method: Using GC-MS as the method,the chemical constituents were analyzed and identified. Result: Twenty components were identified from the total terpene alcohols of A. lances,and the relative contents＞1% of the group were divided into hinesol, β-eudesmol, γ-eudesmol,rugby alcohol and 2,3-Dihydro-1-oxo-phenalene,which hinesol and β-eudesmol were 87%. Conclusion: The total terpene alcohols of A. lances has the highest contents of hinesol and β-eudesmol, and in addition to sesquiterpene alcohol compositions,it also contains sesquiterpene alkene components.关键词：the fifth sort of new drugs;Atractylodes lances;total terpene alcohols;GC-MS
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发布时间：2024-01-04 - 摘要：Objective:To establish an HPLC fingerprint of Euonymus alatus analyzed the chemical constituents what these peaks stood for and compared HPLC fingerprint of E. alatus in different parts. Method: The determination was carried out with Diamonsil C18 (4.6 mm×250 mm, 5 μm) column, using acetonitrile-0.1% acetic acid as mobile phase with gradient elution at a flow rate of 1.0 mL·min-1 and detected at the wavelength of 254 nm and temperature was maintained at 30 ℃. The chromatograms were analyzed with the software of Estimating System of Similarity of 2004A Version (the Country's Pharmacopeia Committee) on the Chinese Medicine Fingerprint Chromatography. Result: The HPLC fingerprint was established by evaluating the chromatogram of 9 samples from different regions and there were 23 common peaks showed in the fingerprint chromatogram. The similarity of 9 batches of E. alatus were 0.824-0.957 in which 6 sources were above 0.900. Conclusion: The constituents in the different parts of E. alatus were so different comparing their HPLC fingerprint that the quality control of E. alatus should be established on the basis of its clinical applications.关键词：Euonymus alatus;HPLC;fingerprint;quality control
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for the content determination of of berberine hydrochloride in prossing Calamina by Sanhuang Tang. Method: HPLC was used with lichrospher C18column (4.6 mm×150 mm, 5μm), acetonitrile-0.1% phosphoric acid (46:64) with mobile phase. The flow rate was 1 mL·min-1;the column temperature was maintained at 30 ℃;the detection wavelength was set at 350 nm. Result: Berberine hydrochloride had a good linearity within 0.042 0-0.525 μg (r=0.999 8) and the average recovery was 95.24% with RSD 1.33%. The content of berberine hydrochloride in three samples of prossing Calamina by Sanhuang Tang was 0.326%, 0.347%, 0.373%. Conclusion: This method is simple, accurate with good reproducibility, and can be used for the content determination of berberine hydrochloride in prossing Calamina by Sanhuang Tang.关键词：prossing Calamina by Sanhuang Tang;berberine hydrochloride;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for determination of total phenols from Phyllanthus urinaria in different locations. Method:Total phenols was determined by UV-Vis spectrophotometer with corilagin as reference substance and FeCl3-K3[Fe(CN)6] as chromogenic agent,the detection wavelength was at 744 nm. Result:A good linear relationship of corilagin was achieved in the range of 0.604-3.02 mg·L-1 (r=0.999 1),the average recovery was 99.79% with RSD of 2.72%(n=6).The content of total phenols from P. urinaria in different locations was different from 5.00% to 12.16%. Conclusion:This established method was simple,accurate and stable which was suitable for determination the content of total phenols from P. urinaria.关键词：Phyllanthus urinaria;total phenols;determination;different locations
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for simultaneous determining of berberine hydrochloride,phellodendrine hydrochloride and matrine in Qingfei Yihuo pills by capillary electrophoresis. Method: With pseudoephedrine hydrochloride as the internal standard,separation was performed on a uncoated fused silica capillary of 55 cm×75 μm(47 cm effective length). 100 mmol·L-1 NaH2PO4+40% ethanol solution(pH 5.75) was selected as the running buffer,separation voltage was 23 kV,samples was injected by gravity in 15 s (15 cm),detection wavelength was at 208 nm. Result: Calibration curves for berberine hydrochloride,phellodendrine hydrochloride and matrine were linear in the concentration range of 40.5-81.0,0.896-3.584,6.0-36.0 mg·L-1.The average recoveries were 96.0%,100.5%,97.8%,respectively,with RSD were not more than 1.85%. Conclusion: This method was accurate and reliable with good repeatability,it could be used for quantitative determination of Qingfei Yihuo pills.关键词：capillary electrophoresis;Qingfei Yihuo Pills;berberine hydrochloride;phellodendrine hydrochloride;matrine
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发布时间：2024-01-04 - 摘要：Objective:To establish qualitative control standard of Baikejing syrup. Method: TLC was used for qualitative analysis of Scutellaria baicalensis,Phellodendron chinense,Citrus reticulata,Ephedra sinica,the content of baicalin was determined by HPLC. Result: Baikejing syrup for samples and reference substances showed the same color dot in corresponding chromatograph position;Sinochrom ODS bp C18 column (4.6 mm×250 mm,5 μm) and ODS pre-column were adopted with mobile phase of methanol-water-phosphoric acid(45∶55∶0.04),detection wavelength was at 315 nm,baicalin showed a good linear relationship within the range of 0.08-0.4 μg(r=0.999 8),the average recovery was 100.9% with RSD of 1.7%. Conclusion: This method was sensitive,reproducible and reliable,it could be used for quality control of Baikejing syrup.关键词：Baikejing syrup;baicalin;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To revise and improve quality standards of Wikstroemia chamaedaphne. Method: According to research methods of 2010 edition of Chinese Pharmacopoeia appendix,microscopic and TLC of W. chamaedaphne was identified,impurities,moisture,ash,acid insoluble ash and the content of active ingredients in 10 batches of W. chamaedaphne were determined. Result: Microscopic characteristics and TLC identification of W. chamaedaphne was determined and established;Moisture limit and impurities limit of W. chamaedaphne were ≤11.0%,acid insoluble ash limit ≤1.0%,ash limit ≤9.0%,the mass fraction of luteolin in 10 batches of W. chamaedaphne was 0.10-1.40 mg·g-1. Conclusion: This study improved quality standard of W. chamaedaphne,it could be as amendments.关键词：Wikstroemia chamaedaphne;quality standard;TLC;HPLC;luteolin
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发布时间：2024-01-04 - 摘要：Objective:To establish a method for determination of salvianolic acid B in Zhongfenghuichun Capsule. Method: HPLC was used to determine the content of salvianolic acid B. A Phenomenex C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was acetonitrile-1% methanoic acid (40∶60). Flow rate was 1 mL·min-1. The detection wavelength was set at 286 nm. The injection volume was 10 μL and column temperature was kept at 30 ℃. Result: The calibration curve of salvianolic acid B was in good linearity over the range of 7.9-196.5 mg·L-1 (r=0.999 9). The average recovery was 99.06% with RSD of 1.54%. Conclusion: The method is simple, accurate and reliable. It can be used for the quality control of Zhongfenghuichun Capsule.关键词：Zhongfenghuichun Capsule;salviandic acid B;HPLC;content determination
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发布时间：2024-01-04 - 摘要：Objective:To determine the residues of heavy metals and harmful elements in Aconiti Radix. Method: Samples were analyzed by ISIS-DS-HMI-ICP-MS. Result: Under the optimized experimental conditions, the detection limits were in the range of 0.003-0.386 μg·L-1. The recoveries were in 80.75%-111.41%. Conclusion: The method is sensitive, simple and accurate. It can be used for continuous determination of Aconiti Radix.关键词：Aconiti Radix;ISIS-DS;HMI;ICP-MS
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发布时间：2024-01-04 - 摘要：Objective:To develop a quantitative method for determining the contents of baicalin and icariin in Fuzheng Guben granule. Method: The analysis was performed with a Ultimate AQC18(4.6 mm×250 mm,5 μm) column and a mobile phase of acetonitrile-0.2% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL·min-1, and column temperature was 25 ℃. The detection wavelength was set at 270 nm. Result: The calibration curve was linear within the range of 0.756-10.08 μg for baicalin(r=1.000 0) and 0.299-39.92 μg for icariin(r=0.999 6). The average recoveries of the two contents were 101.40%(RSD 1.41%)and 101.02%(RSD 1.08%) respectively. Conclusion: This method is convenient, accurate and reliable, and can be used as quality control methods for Fuzheng Guben granule.关键词：Fuzheng Guben granule;baicalin;icariin;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To develop a method for quality control of Fufang Kushehuang Lotion. Method: Radix Sophorae Flavescens, Cnidium monnier and Cortex Phellodendri were identified by TLC. HPLC was used for determination of matrine. Result: The chromatographic spots were identified without interference of negative control. Matrine had a good linearity within the range of 0.308-3.080 μg (r=0.999 8) with average recovery of 99.52% (RSD 0.55%). Conclusion: This standard is used for quality control of Fufang Kushehuang Lotion.关键词：Fufang Kushehuang Lotion;matrine;TLC;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC method for determination of three constituents in Qingfei Yihuo Pills. Method: Baicalin, geniposide and genipin-1-O-β-gentiobioside were determined simultaneously by HPLC on a agilent Zorbax SB-C18 column with the methanol(A)-1.0% formic acid anhydrous(B)-acetonitrile(C) in gradient elution as the mobile phrase. The detection wavelength was set at 238, 440 nm and the flow rate was 1.0 mL·min-1. Result: The standard curves for baicalin, geniposide and genipin-1-O-β-gentiobioside were linear within the range of 0.165-1.650, 0.106-1.060, 0.035-0.350 μg (r>0.999 7),and the average recoveries were over 96.75% (RSD<2.05%). Conclusion: The method is simple and accurate and could be used for quality control of Qingfei Yihuo Pills.关键词：Qingfei Yihuo Pills;baicalin;geniposide;genipin-1-O-β-gentiobioside;content determination
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发布时间：2024-01-04 - 摘要：Objective:Study on the content of main active compounds in the compatibility Ephedra Herba with Radix Aconiti Lateralis in Mahuang Fuzi Xixin decoction and Mahuang Fuzi Gancao decoction,provide experimental basis for scientific prescripti compatbility. Method: HPLC was adopted to analyzed the content of norpseudoephedrine (NMP), norephedrine (NME), ephedrine (E), pseudoephedrine (PE), methylephedrine (ME), Benzoyl mesaconine and Benzoyl hypacoi-tine which are the major active compounds of compatibility Ephedra Herba with Radix Aconiti Lateralis. Result: The five alkaloid of Ephedra Herba showed little change, but had significant difference in active compounds of Radix Aconiti Lateralis. Conclusion: The content of main active compounds in the compatibility Ephedra Herba with fuzi in two decoction had difference related to its clinical application, but the exact cakse in not clear.关键词：Ephedra Herba;Radix Aconiti lateralis;compatibility;Mahuang Fuzi Xixin decoction;Mahuang Fuzi Gancao decoction
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发布时间：2024-01-04 - 摘要：Objective:To examine incorporation of Western medicine into vegetable drugs (traditional Chinese medicine) by using UV absorption spectrum. Method: Nine chemicals were respectively mixed into Siwu decoction(made from Prepared Rehmannia Root, White Peony Alba, Chinese Angelica Root and Chuanxiong Rhizome). Using weighted correlation analysis (based on the correlation, with larger weighted value around peaks and valleys of UV absorption spectrum) to analyze the variation on spectrum between before and after the mixing. Result: Six chemicals influenced UV absorption spectrum significantly after they were respectively mixed into Siwu decoction, the similarity between before and after mixing was low with Spearman'sρ<0.94, but the other 3 chemicals was not, with Spearman'sρ>0.95. Conclusion: UV absorption spectra can be used for identifying the chemicals mixed illegally into decoction of traditional Chinese medicine.关键词：Traditional Chinese Medicine preparation;SIWU decoction;UV absorption spectrum;weighted correlate;mixing
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发布时间：2024-01-04 - 摘要：Objective:To establish a HPLC fingerprint method of Sargentodoxa cuneata, for its quality control. Method: The separation was performed by RE-HPLC chromatography Ultimate XB-C18 (4.6 mm× 250 mm, 5 μm) column with a mobile phase of acetonitrile-methanol (1∶1) and 0.1% phosphoric acid water at a flow rate of 0.6 mL·min-1 and 25 ℃, gradient elution. The detecting wave length was 280 nm. The Milettia reticulata and Longpeduncle kadsura stem fingerprints were also detected for similarity comparison. application system cluster analysis to classify different sources S. cuneata herbs. Result: The HPLC fingerprint analysis method of S. cuneata was established and Calibration of 22 common peaks,identified three peaks:salidroside, protocatechuic and Rhein. There were significant differences in fingerprint chromatogram of S. cuneata, M. reticulata and L. kadsura stem.11 batches of different sources S. cuneata herbs can be divided into three categories by Hierarchical cluster analysis. Conclusion: The method is simple, accurate, reproducible, and can be used to the S. cuneata fingerprint research.关键词：Sargentodoxa cuneata;HPLC;fingerprint;identify;hierarchical cluster analysis
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发布时间：2024-01-04 - 摘要：Objective:To establish the inter simple squence repeat(ISSR)-PCR reaction system of Tupistra chinensis. Method: The total genome DNA of T. chinensis. was used as the material. The concentration of Mg2+, dNTP, primer and Taq DNA polymerase, which greatly influence ISSR-PCR reaction, were optimized. Result: The optimized reaction conditions was established as follows:in a volume of 10 μL containing 1×buffer, 2 U Taq DNA polymerase, 2 mmol·L-1 MgCl2, 0.2 mmol·L-1 dNTP, 0.75 μL primer. Conclusion: Sixteen primers with stable amplification and rich polymorphism were screened from 80 (inter simple sequence repeat)ISSR primers. The optimized annealing temperature for each primer in ISSR-PCR reaction was proposed. This study will be helpful for research on the genetic diversity analysis and germplasm identification of T. chinensis.关键词：Tupistra chinensis;inter simple sequence repeat;reaction system
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发布时间：2024-01-04 - 摘要：Objective:To isolate and identify the chemical constituents in the whole plant of the Petasites japonicus, and to provide samples for activity screening. Method: The whole plant of P. japonicus was extracted with MeOH. And column chromatographic techniques were used and the structures of the constituents were elucidated on the basis of physicochemical properties and spectral data. Result: Five compounds were obtained and identified as valerilactones A ( 1 ), bakkenolide-Ⅴa( 2 ), bakkenolide-Ⅳa( 3 ), bakkenolide-Ⅲa( 4 ), homofukinolide ( 5 ). Conclusion: Compounds 1 were obtained from P. genus for the first time.Compounds 2,3,4 were obtained from the whole plant of P. japonicus for the first time.关键词：Petasites japonicus;chemical constituents;structure identification
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发布时间：2024-01-04 - 摘要：Objective:To study the microstructure of stems and leaves of Artabotrys hexapetalus and distribution characteristics of endophytic fungi in the tissues, and to isolate and identify these endophytic fungi from the stem and the leaf of A. hexapetalus. Method: Permanent paraffin-cut section, optical microscope photography and histochemistry were used to study the location of endophytic fungi. The solidified potato dextrose agar(PDA) of plates was applied for the isolation of the endophytic fungi, and the identification was completed by the internal transcribed spacer (ITS) sequence. Result: The secondary structure of the stems of A. hexapetalus consisted of the periderm, cortex and vascular cylinder (stele). Sieve and tubes phloem fibers, scattering in bundles, arranged at intervals. The leaves of A. hexapetalus were of typically bifacial, consisted of epidermis, mesophyll and veins. The midrib vascular bundle, collateral vascular bundle, was ring with vascular bundle sheath. Endophytic fungi existed in the local cells of cortex, phloem, palisade tissue and spongy tissue. A total of 18 strains was isolated and identified, among them Phomopsis spp. accounted for 61.1% in all strains of endophytic fungi. Conclusion: The microscopic characteristics of A. hexapetalus's stem and leaf can be used as a reference for identification. The endophytic fungi of A. hexapetalus were distributed with no obvious tissue specifity and P. spp. were determined as dominant species.关键词：Artabotrys hexapetalus;endophytic fungi;histochemistry;ITS;identification
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发布时间：2024-01-04 - 摘要：Objective:To study the suitable harvest time of Scutellerir baicalensis in Ningxia liupan mountain. Method: HPLC was used to compare the content of radix baicalin from different time of the collection and different strains of the age, at the same time to compare the alcohol-soluble extract content. Result: S. baicalensis baicalin cultivated in October had the highest,content, alcohol-soluble extract content from S. baicalensis baicalin cultivated during the period of July to September was growing rapidly, the highest content was found in October, adixscutellariae root weight (October) with three years life had higher tcontent han the biennial. Conclusion: The optimμm collection time for ultivating S. baicalensis was October in the third year of growth.关键词：Ningxia liupan mountain cultivating Scutellerir baicalensis;suitable harvest time;baicalin;HPLC
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发布时间：2024-01-04 - 摘要：Objective:To evaluate dynamic accumulation of active ingredient in different part and growth phase of caltivative Zanthoxylum nitidum, and provide the basis for the GAP planting and the rational utilization. Method: A sensitive and accurate method was developed to ditermined the nitidine chloride in different parts (root, stem, and branch) and different harvest times of Z. nitidum by ultra performance liquid chromatography (UPLC) with diode array detector (DAD) for the first time. Result: The variation of the nitidine chloride contents was existed in different parts of Z. nitidum, which sequence was showed as follows:root>stem>branch,there was regulation dynamic changes of nitidine chloride content in different part and growth phase, which exist two obvious peak and a valley, that is the content of nitidine chloride reached highest value at May and October and lowest at June. Conclusion: The active ingredient distributed in caltivative Z. nitidum was different in different part and growth phase, the optimal harvest time was at October and November in the third-year, and the root was the main medicinal parts. All part of Z. nitidum have the medicinal value.关键词：caltivative Zanthoxylum nitidum;nitidine chloride;dynamic accumulation;UPLC-DAD;content;harvest time
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发布时间：2024-01-04 - 摘要：Objective:To investigate effect of Glycyrrhiza uralensis on in vivo metabolites of triptolide and triptonide,disclose attenuation mechanism of G. uralensis on Tripterygium wilfordii. Method:SD rats were divided into single and combined administration groups.Combined groups were injected with triptolide or triptonide after orally treated with extracts of G. uralensis,while these single groups was injected only with triptolide or triptonide. Metabolites of triptolide or triptonide in rat plasma were identified and determinated by LC-MS/MS,and compared difference of metabolites under two administration models. Result:Four metabolites ([M+H]+,m/z 359,359,375,377) of triptolide and three metabolites ([M+H]+,m/z 375,397,413) of triptonide in rat plasma were found under two administration models,respectively;Oxydation metabolites (m/z 359 of triptolide,m/z 375 of triptonide) were the major metabolites.Another metabolite ([M+H]+,m/z 413) of triptonide in rat plasma were found only in these combined administration groups.Difference of the amount of metabolites under two administration models was significant,combined administration model was the larger. Conclusion:Under two administration models,most of metabolites of triptolide and triptonide in rat plasma were the same,while major metabolites was oxidative metabolites.G. uralensis could accelerate in vivo metabolism of triptolide and triptonide,it could provide a reference for clarifying attenuation synergistic mechanism of T. wilfordii.关键词：Glycyrrhiza uralensis;triptolide;triptonide;metabolites;LC-MS/MS
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发布时间：2024-01-04 -
Simultaneous Determination of Four Kinds of Alkaloid from Rhizoma Coptidis in Rat Plasma by LC-MS/MS
摘要：Objective:To develop a LC-MS/MS method for simultaneous determination of jatrorrhizine,coptisine,palmatine and berberine in rat plasma. Method: Taking ACQUITY UPLC BEH C18column(2.1 mm×50 mm,1.7 μm) with mobile phase of acetonitrile-water (2 mmoL ammonium acetate and 0.05% formic acid),flow rate was 0.3 mL·min-1,column temperature was 30 ℃.Strychnine was adopted as the internal standard,plasma samples were pretreated by protein precipitation with 4 times the amount of acetonitrile,electrospray ionization(ESI) was applied and operated in positive ionmode,quantification was performed by multiple reaction monitoring(MRM) of jatrorrhizine m/z 338.227→m/z 322.824,coptisine m/z 320.244→m/z 291.996,palmatine m/z 352.243→m/z 308.016,berberine m/z 336.227→m/z 277.981 and the internal standard m/z 335.264→m/z 184.017,respectively. Result: Calibration curve of jatrorrhizine,coptisine,palmatine and berberine in plasma were linear in the range of 1.01-505,1.06-530,1.13-565,1.07-535 μg·L-1;Lower limit of quantitation were 0.539 2,0.646 3,0.761 5 and 0.197 1 μg·L-1,respectively;Average recoveries were in order of 103.90%,101.50%,101.58% and 115.54%,respectively.RSD of intra-and inter-day,stability were less than 10%. Conclusion: This assay method was accurate,sensitive,specific and suitable for simultaneous determination and pharmacokinetic study of jatrorrhizine,coptisine, palmatine and berberine in rat plasma.关键词：LC-MS/MS;jatrorrhizine;coptisine;palmatine;berberine;plasma concentration- 2
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发布时间：2024-01-04 - 摘要：Objective:The absorption characteristic of the icariin(ICA), which is the main component of Naoyiyuan tables, was clarified by the membrane transport study. Method: The Caco-2 monolayer was used to study the transport of the ICA in monomer, herbs, and compounds in vitro.For the determination of the content of ICA, the HPLC method was operated with acetonitrile-water(30∶70) as mobile phase, with Luna Kromasil C18 column. The flow rate was 1.0 mL·min-1, detection wavelength was at 270 nm. Result: ICA exhibited time-dependent permeation with apparent permeability coefficient P-gp values in the range 1×10-5-1×10-6cm·s-1, suggesting transcellular diffusion pathways. P-glycoprotein may play a role in the intestinal efflux of icariin. However compared with the icariin in monomer and herbs, the transport of ICA in Naoyiyuan tables is higher. Conclusion: The intestinal efflux of ICA in Naoyiyuan tables was reduced. So we can considered that the other ingredients in Naoyiyuan tables inhibited the efflux of icariin. It is profit for the pharmacological actions.关键词：Naoyiyuan tables;Caco-2 cell;transport;icariin
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发布时间：2024-01-04 - 摘要：Objective:To compare the differences between the asthenia-cold syndrome in patients with primary dysmenorrhea and normal group in urinary endogenous metabolites, and elaborate the path physiological mechanisms of the asthenia-cold syndrome in patients with primary dysmenorrheal. Method: N-methyl-N-(trimethylsilyl) tri-fluoroacetamide (MSTFA) was selected as derivatization reagent to detect urine metabolites at the second day in menstrual cycle (MC2) of the two groups using gas chromatography-time of flight mass spectrometer (GC-TOFMS), drawn the total ion chromatogram(TIC) of each group, combination with unsupervised principal component analysis (PCA) for the multi-dimensional statistical analysis to look for variability material between the two groups. Result: Compared with the normal group, twelve variability materials in asthenia-cold group were detected. They were fructose, maltose, arbinofuranose, isocitric acid, lactic acid, hippuric acid, glycine, serine, threonine, palmitic acid, octadecanoic acid, and erythritol. Among the total materials only lactic acid in asthenia-cold group was higher than in the normal group;the rest materials in asthenia-cold group were all lower than in the normal group. Conclusion: Asthenia-cold syndrome of the primary dysmenorrheal patients and the normal group suggested significant difference in urine metabolites. These variability materials are related to several metabolic pathways such as glycolmetabolism, amino acid metabolism, fatty acid metabolism and disturbance of enterobacteria.关键词：asthenia-cold syndrome;primary dysmenorrhea;metabolism;urine
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发布时间：2024-01-04 - 摘要：Objective:To investigate the major effective compositions in Jianpi Huoxue decoction against lipid peroxidation which can protect alcohol-induced liver injury remarkably and its possible action mechanism. Method: The rats were given Lieber-DeCarli liquid diet daily to make the alcoholic liver injury model. And Uniform design was adopted to design the experiments. malonyldialdehyde(MDA) in liver tissue was detected as the index. The same mode was used to screen the major herbs and verify the efficacy. The superoxide dismutase(SOD),nitric oxide synthase(NOS),induced nitric oxide synthase(iNOS),glutathione peroxidase(GSH-Px)in liver and Fe2+ in serum were detected. Result: Puerariae Lobatae Radix,Alismatis Rhizoma, Schisandrae Chinensis Fructus, and turmeric were the major herbs in Jianpi Huoxue decoction against lipid peroxidation. And the verification experiment showed that compared with the model group, the major herbs could reduce the MDA,NOS,iNOS and Fe2+ significantly and promote the activity of SOD,GSH-Px apparently. Conclusion: The combination of Puerariae Radix,Alismatis Rhizoma, Schisandrae Chinensis Fructus, and turmeric were the major effective compositionsof Jianpi Huoxue decoction against lipid peroxidation. The possible action mechanism may lie in inhibiting NOS,iNOS and Fe2+ in serum which promote oxidation, and increase the activity of free radical scavenger SOD,GSH-Px which provide anti-oxidation.关键词：Jianpi Huoxue decoction;lipid peroxidation;the major effective herbs;action mechanism;uniform design
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发布时间：2024-01-04 - 摘要：Objective:To observe the expression of core binding factor alpha 1 mRNA of femoral organization by Zuogui Pill in ovariectomy-induced osteoporosis rats. Method: Two hundred and eighty male SPF SD rats were divided into three groups:normal group 40;sham-operated group 40;ovariectomy-induced osteoporosis model was adopted, 21 days later, randomly modeling rats were divided into 5 groups:the model group, nilestriol group, Zuogui Pill high, medium, low dose group. Zuogui Pill groups were ig given with Zuogui Pill at dosage of 6.4, 3.2, 1.6 g·kg-1, once per day. Nilestriol group was treated with Nilestriol liquid. The normal group, sham-operated group and the model group were given with distilled water.60, 120, 180 d later, the expression of core binding factor alpha 1 in the femoral organization was determined by RT-polymerase chain reaction (PCR). Result: Compared with the normal group, in the model group the femoral core binding factor alpha 1 mRNA level obviously reduced (P<0.01). And compared with the model group, Zuogui Pill groups increased femoral core binding factor alpha 1 mRNA level significantly(P<0.05). Conclusion: The down-regulated expression of core binding factor alpha 1 mRNA of bone tissue may be contributed to PMOP, Zuigui Pill can up-regulated mRNA expression.关键词：Zuogui Pill;osteoporosis;core binding factor alpha 1
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of Mahuang Jiazhu decoction on the serum interleukin-2(IL-2)and interferon-γ(IFN-γ) content of mice infected by RSV in the damp and cold environment,and the correlation of immune state of mice with damp and cold environment and chinese and western drugs under ecological medicine. Method: The serum IL-2 and IFN-γ contents were examined with ELISA in mice which were placed artificial climate box to mimic the environment of simulating cold-wet exopathogen,to analyze the effects of Mahuang Jiazhu decoction and cold-wet exopathogen on immune state of mice infected by RSV. Result: The IL-2 and IFN-γ contents in virus model group and cold-wet group were lower significantly (all P<0.001)(all P<0.001) than that in normal group. There was difference in IL-2,IFN-γ contents between virus model group and cold-wet groups (P<0.001,P<0.05). The IL-2 and IFN-γ contents in the Mahuang Jiazhu decoction group and Ribavirin group were higher significantly than that in virus model group and cold-wet plus virus model groups, respectively(P<0.001).But there was no difference in the IL-2 content between Mahuang Jiazhu decoction group and Ribavirin group.The IFN-γ contents in virus plus Mahuang Jiazhu decoction group was lower than that in virus plus Ribavirin group(P<0.001). The IFN-γ contents in cold-wet plus virus plus Mahuang Jiazhu decoction group was lower than that in virus plus Mahuang Jiazhu decoction group(P<0.01). Conclusion: The decreased immunity of mice can be caused cold-wet exopathogen and virus infection. Mahuang Jiazhu decoction and Ribavirin can improve the immune state of mice infected by RSV in the damp and cold environment.关键词：artificial climate box;upper respiratory tract infection;cold-wet exopathogen;IL-2;IFN-γ;ecological etiology
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发布时间：2024-01-04 - 摘要：Objective:To observe the impact of Qingchang Huashi formula(QHF) on mouse bone marrow-derived dendritic cell(DC) antigen-presenting function and explore the mechanism of action of the treatment of UC. Method: The DCs ftransfected by nuclear factor κB decoy oligonucleotides(NF-κB ODN) were as control group to observe the change the characters of the DC after incubation of QHF,and then to observe whether QHF can influence the function of DC antigen-presenting by inhibiting the expression of NF-κB. The experiment was divided into six groups,which is blank group, QHF group, ODN transfection group, QHF and lipopolysaccharides(LPS) group, ODN transfection and LPS group. Flow cytometry was used to detect the DC surface of CD11c, CD40, MHC II expression and immune fluorescence was used to detect the nuclear translocation of NF-κB of each group. Result: QHF could effective ly reduce the DC surface antigens CD40 and MHC II costimulatory molecule expression, and inhibit NF-κB activation into the nucleus. Conclusion: By inhibiting the expression of NF-κB, affecting maturation and differentiation of DC,reducing the antigen-presenting function,thereby reducing the inflammatory response are the main mechanism of QHF treating UC.关键词：Qingchang Huashi Formula;DC;UC;NF-κB
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发布时间：2024-01-04 - 摘要：Objective:To study the protection and treatment effects of Buqing granules on galactose cataract. Method: Animal model of galactose cataract was induced in SD rats.The rats were divided into six groups:black control group, model group treated by daily intraperitoneal injection of 50% D-galactose solution (10 g·kg-1) for 15 days,positive control group treated by Zhangyan uspension 0.02 g·kg-1 everyday,high, medium and low dose groups of Buqing granules (0.8, 0.4, 0.2 g·kg-1). Recorded the lens turbidity was recorded before and after modeling from the first day to 3,7,14.At the end of the experiment,superoxide dismutase (SOD) and malondialdehyde (MDA) was detected. Result: The lens in control group was transparent, in model group lens opacities occurred at the third day,in the three dose groups of Buqing granules occurring time and extent of lens opacities at 3, 7 and 14 days were significantly lower than the model group(P<0.05). SOD activity was significantly higher than the model group(P<0.05),MDA was significantly lower than that in the model group (P<0.05). Conclusion: The Buqing granules had better prevention and treatment in rats with galactose cataracts.关键词：Buqing granules;galactose cataract;superoxide dismutase;malondialdehyde
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发布时间：2024-01-04 - 摘要：Objective:The effects of Yulangsan polysaccharides(YLSP) on Caspase-3 expression and activity of the frontal lobe and hippocampus in senescence accelerated-prone mouse 8(SAMP8) mice brain were investigated. Method: The SAMP8 mice were adopted as the Alzheimer's disease model group, and SAMR1 as the control group. Both groups were randomized and given 0.5%CMC-Na solution. Huperzine(0.02 mg·kg-1) was given to drug-positive group and two YLSP groups were given 45, 180 mg·kg-1 respectively, continuous administration for 40 days. In the frontal lobe and hippocampus, Caspase-3 expression and activity were detected respectively by immunohistochemical method and Caspase-3 activity assay kit. Result: In model group, Caspase-3 expression and activity were obviously higher than that in the control group(P<0.01). In drug-positive and YLSP groups, Caspase-3 expression and activity were obviously lower than that in model group(P<0.01). YLSP presented a dose-dependent manner (P<0.05). Conclusion: The abnormal Caspase-3 expression and activity can be reduced by YLSP, and it has the protective function towards neural cell apoptosis in SAMP8 mouse brain.关键词：Yulangsan polysaccharides;SAMP8;Caspase-3;apoptosis
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发布时间：2024-01-04 - 摘要：Objective:To research the effect of 6-hydroxy justicidin A(JR6)on endoplasmic reticulum stress(ERS) and Ca2+ in HepG2 cells. Method: Semiquantitative RT-PCR and Western blot were used to investigate the expression levels of X-box binding protein 1(XBP-1) mRNA, Inositol-requiring enzyme-1α(IRE1α), Glucose regulated protein/BiP(GRP78/BiP), B cell leukemia 2(Bcl-2) proteins in HepG2 cells which were treated with 0,0.615,2.45,9.8 μmol·L-1 JR6 separately for 24 h. Laser confocal scanning microscopy was used to study the difference of intracellular calcium between medicine group and IP3R(inositol 1,4,5-trisphos-phate receptor) inhibited group. Result: The expression of IER1, GRP78/BiP Bip, Bcl-2 proteins and XBP-1 mRNA have a reduction in approximately 40% with high JR6 group. It is found that the JR6 could elevate the intracellular calciumion concentration more than two times, and this effect was disappeared with the IP3R inhibitor (Xestospongin C). Conclusion: JR6 through IP3R way elevates intracellular free Ca2+ concentration, and arouse ERS. At the same time, JR6 can inhibit elements which related to unfolded protein response(UPR).关键词：HepG2;tumor;endoplasmic reticulum stress;intracellular free Ca2+ concentration;unfolded protein response
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发布时间：2024-01-04 - 摘要：Objective:To study the effects of baicalin on the proliferation, invasion and migration of A549 cells, and tube formation of Human umbilical vein endothelial cells (HUVEC)on matrigel in vitro, as well as the antitumor effect of baicalin on A549 cell xenografts in vivo. Method: A549 cells were treated by baicalin within different concentrations (25,50,100,200,400 μmol·L-1)24,48,72 h respectively, the cell density was 1×104/mL, cell proliferation was validated by Thiazolyl blue terazolium bromide (MTT) method. The changes of cell invasion and migration in vitro were detected by Transwell assay, the anti-angiogenic effect was detected by tube formation experiment, the concentration of baicalin of the three experiments were 12.5,25,50 μmol·L-1. Antitumor efficacy of baicalin was evaluated in nude mice models of human lung cancer xenograft, the nude mice were divided into 4 groups, the high dose group of baicalin (100 mg·kg-1), the low dose group of baicalin (50 mg·kg-1),the solvent control group and the cisplatin(1 mg·kg-1) group. The administration method of the three former groups was by gavage for 21 days consecutively, the cisplatin group was by intraperitoneal injection for 10 days consecutively. Result: ①After treated with different concentrations of baicalin for 24, 48, 72 hour, the growth of A549 cells were significantly inhibited(P<0.05 to P<0.01), the IC50of baicalin in different times were 100.77, 75.02, 55.81 μmol·L-1 respectively. ② Baicalin(25,50 μmol·L-1) could inhibit the invasion and migration of A549 cells, therefore, it inhibit the metastatic of cancer cells. ③The tubule structure formation treated with baicalin(25,50 μmol·L-1) was reduced compared with the control group. ④ Baicalin(50 mg·kg-1) could inhibit the A549 cell xenograft in vivo. Conclusion: These results suggest that baicalin could inhibit the proliferation, invasion and migration of A549 cells and the tube formation of Human umbilical vein endothelial cells in vitro, in vivo baicalin could inhibit the A549 cell xenograft, its mechanism on lung cancer need further study.关键词：baicalin;A549 cells;proliferation;invasion;migration;angiogenesis;xenograft
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发布时间：2024-01-04 - 摘要：Objective:Tostudy the mechanism of shikonin inducing K562 cells apoptosis. Method: MTT colorimetric assay was used to examine the proliferation inhibition of shikonin on K562 cells. Hoechst 33258 and acridine orange(AO)/ethidium bromide(EB) fluorescent staining were used to observe the morphological changes of apoptotic cell. The mRNA expression levels of B-cell lymphoma gene 2 associated X protein(Bax), BH3 interacting domain death agonist(Bid), B-cell lymphoma gene xL(Bcl-xL), p53 up-regulated modulator of apoptosis(PUMA), B-cell lymphoma gene 2 homologous antagonist(Bak), B-cell lymphoma-w(Bcl-w) and B-cell lymphoma gene 2 associated death promoter(Bad) were detected using RT-PCR analysis. The changes of reactive oxygen species(ROS) and reduced GSH were detected using molecular probe DCFHDA and CMFDA:CMFDA, separately. Result: Shikonin(0.2-1.6 mg·L-1)inhibited K562 cell proliferation in concentration-dependent manner, and induced cell apoptosis. Typical apoptotic changes were observed in K562 cells under the shikonin(0.2-0.5 mg·L-1) treatment groups. The expression of proapoptotic protein (Bid, Bad, Bak, PUMA, Bax) was increased and antiapoptotic protein (Bcl-w, Bcl-xL) decreased. ROS formation increased, but reduced glutathione level was decreased. Conclusion: Shikonin can inhibite K562 cell proliferation and induce apoptosis through inducing oxidative stress.关键词：shikonin;K562 cell;apoptosis;reactive oxygen species
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发布时间：2024-01-04 - 摘要：Objective:To study the antidepressant effects of Albizzia Julibrissin Flower total flavonoids (AJFTF) and explore its mechanism preliminarily. Method: Ninty SD rats were randomly divided into normal group, model group, venlafaxine hydrochloride group (0.012 5 g·kg-1), and high, middle, low dosages of AJFTF groups(0.10,0.05,0.025 g·kg-1). Depression model rats were induced by solitary cultivation plus chronic stress. Rats in venlafaxine hydrochloride and AJFTF groups were orally administered with Venlafaxine Hydrochloride or AJFTF once daily for 21 days, and rats in normal and model groups with equal volume distilled water. The behavior changes were determined by open field test, and the behavior and hippocampal CA3 neurons apoptosis were determined by TUNEL assay. Result: Compared with the normal group, horizontal movements and vertical movements scores in 3 min of open field test in model group were significantly lower (P<0.05 or P<0.01), and the numbers of apoptotic and positive cells in hippocampal CA3 region were significantly increased (P<0.01).Compared with the model group, 3 min horizontal movements and vertical movements scores of open field test in venlafaxine hydrochloride group, AJFTF (0.10,0.05,0.025 g·kg-1) dosage groups were significantly higher (P<0.05 or P<0.01), and the numbers of apoptotic and positive cells in hippocampal CA3 region were significantly reduced (P<0.05). Conclusion: AJFTF has antidepressant effects, and its mechanism may be related to antagonizing the hippocampal neurons apoptosis of CA3 region.关键词：Albizzia Julibrissin Flower total flavonoids (AJFTF);depression;apoptosis
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effects of Ziwan Wan on the hemorheological parameters, clotting time and lung presentations in rat fevered and bleeding model. Method: The SD rats were divided into blank control group, model compared group, Yunnan Baiyao 0.5 g·kg-1 group, Ziwan Wan (high, middle and low, 5, 2.5, 1.25 g·kg-1 accordingly) dose group. The corresponding drugs were iggiven with dose of 20 mL·kg-1 twice a day, for 7 days. Except the blank control group, fevered and bleeding models were established. The hemorheological parameters, thrombin time(TT), activated partial thromboplastin time(APTT), the prothrombinase time(PT), fibrinogen(FIB) and the signs of lung presentations were detected in these groups. Result: In the model group compared with blank control group,of whole blood viscosity and plasma viscosity were increased, TT, APTT and FIB were prolonged, PT significantly was shorten. The middle dose of Ziwan Wan could dramatically lower whole blood viscosity, plasma viscosity, APTT, TT and increase the content of FIB. The high dose of Ziwan Wan could significantly shorten the TT, Low dose of Ziwan Wan could significantly prolong PT. The whole blood viscosity, APTT, TT and FIB in the middle dose of Ziwan Wan group was respectively 200 s-1(5.50±0.15) mPa·s, 30 s-1(7.64±0.19) mPa·s, 5 s-1(13.70±0.51) mPa·s,1 s-1(32.38±2.06) mPa·s, APTT(17.11±0.78)s, TT(30.07±2.00)s, FIB(5.56±0.14) g·L-1;TT of high dose of Ziwan Wan group was that TT(30.74±2.96)s;PT of low dose of Ziwan Wan group was that PT(14.63±1.14)s, compared with the model group, P<0.05 or P<0.01.Ziwan Wan could improve lung injury related symptoms in fevered and bleeding model groups, The middle dosage showed the best effect. Conclusion: The middle dose of Ziwan Wan showed the effect of Cooling blood and hemostasis by ameliorating the abnormal hemorheological parameters. The middle dose of Ziwan Wan is effective for hemostasis, and plays roles by ways of endogenous thrombin, extrinsic thombin and fibrin. The high dose of Ziwan Wan has certain effect for part of the abnormal hemorheological parameters and hemostasis parameters. Three doses of Ziwan Wan showed therapeutical effect in damaged lungs in fevered and bleeding model.关键词：Ziwan Wan;fevered and bleeding animal model;hemorheology;hemostasis;lung presentation
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发布时间：2024-01-04 - 摘要：Objective:To study the anti-cancer activity of two flavonoids 4'-methoxy-eupatolitin-3-O-β-D-glucoside(compoundⅠ)and kaempfrol-3,7-O-α-L-di-rhamnoside(compoundⅡ) in the leaf and stem of Tagetes erecta. Method: SGC7901 and SMMC7721 cells were treated by 20,40,80,120,160 μmol·L-1 two flavonoids for 24,48,72 h, MTT assay was used to determine the inhibition rates,and 5-FU was used as positive control. AO/EB double fluorescent staining was used to observe the changes of cell morphology and the apoptosis of tumor cells at the concentration of 1×106/mL after treated with 160 μmol·L-1 of two flavonoids for 48 h. Result: The different concentrations of two flavonoids could both inhibit the proliferation of SGC7901 and SMMC7721 in the time-and dose-dependent manner.The IC50 of compoundⅠ was 111.7 mg·L-1 and 330.4 mg·L-1,respectively. The IC50 of compoundⅡwas 683.8 mg·L-1 and 464.7 mg·L-1,respectively.The morphology of two tumor cancer cells was changed after treated by two compounds,and part of them emerged apoptosis. Conclusion: Two flavonoids separated from the leaf and stem of T. erecta have the activity of anti-liver cancer and anti-gastric cancer in vitro.关键词：Tagetes erecta;flavonoid;MTT;fluorescence staining
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发布时间：2024-01-04 - 摘要：Objective:To observe effect of Jiawei Danshen Yin(JWDSY) on the expression of basic fibroblast growth factor (bFGF) in the edge of infarcted myocardium in the rats with ischemia reperfusion injury(IRI) which was transplanted in bone mesenchymal stem cells(BMSCs). Method: BMSCs were cultivated by bone marrow adherent method. The phenotypes of CD90, CD11b, and CD45 were analyzed with flow cytometer. The third generation cells were treated with blank serum or containing JWDSY serum. Rats were modeled as IRI, and then BMSCs were transplanted. In 3 d, 7 d, 14 d, respectively, specimens were taken from each group, myocardial bFGF were detected. Result: IA value of containing JWDSY serum group after 3,7,14 day increased to 5 758.18±465.17,6 589.61±432.89,8 015.62±366.34 respectively. Compared with sham operation groups, the IA value of the rest groups were raised as time gradually, the difference had statistics meanings (P<0.05). Conclusion: The results suggested that BMSCs intervened by containing JWDSY serum could protect the myocardial cells in the IRI rats.关键词：Jiawei Danshen Yin;BMSCs;basic fibroblast growth factor;IRI;medicated serum
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发布时间：2024-01-04 - 摘要：Objective:To investigate the inhibitory effects of 17-methoxyl-7-hydroxyl-benzofuran chalcone(YLSC) on platelet aggregation and coagulation function in rats. Method: Fifty SD rats were randomly divided into five groups:control group, aspirin group(10 mg·kg-1) and YLSC groups(2.5, 5,10 mg·kg-1). YLSC and aspirin were administrated through sublingual vein for one week.Inhibition rates of platelet aggregation by YLSC in vivo and vitro were determined in the model of platelet aggregation induced by adenosine diphosphate (ADP), collagen and arachidonic acid(AA).The anticoagulant effect of YLSC was evaluated by using thrombin time(TT), prothrombin time(PT), and activated partial thromboplastin time(APTT) assays. Result: Compared with the control group, YLSC could significantly inhibit platelet aggregation induced by ADP and collagen(P<0.05 or P<0.01), but did not inhibit platelet aggregation induced by AA. YLSC significantly prolonged the TT and APTT in a dose-dependent manner(P<0.05 or P<0.01). Wereas, YLSC did not show significant prolonging effect in PT assays. Conclusion: YLSC can exert remarkable effects against platelet aggregation and coagulation.关键词：17-methoxyl-7-hydroxyl-benzofuran chalcone;platelet aggregation;prothrombin time;activated partial thromboplastin time;thrombin time
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发布时间：2024-01-04 - 摘要：Objective:To observe the improvement effect of Yilong Tongbi decoction on chondrocyte apoptosis regulatory gene expression of cartilaginous tissue on the rabbit models of knee osteoarthritis (KOA), and elucidate its potential mechanism. Method: Forty healthy male rabbits were randomly divided into four groups:normal control group, model group, Yilong Tongbi decoction group and glucosamine hydrochloride capsules group. The right knee osteoarthritis model was induced by anterior cruciate ligament transaction except the normal control group. The modeling rabbits were treated by gavage with Yilong Tongbi decoction (0.6 g·kg-1·d-1) and glucosamine hydrochloride capsules (0.1 g·kg-1·d-1) for 4 weeks. The following indexes was observed in all rabbits at experimental endpoint that the ultrastructure changes of arthrodial cartilage and synovium including levels of interleukin (IL)-1β and tumor necrosis factor-α (TNF-α)in synovial fluid by enzyme linked immunosorbant assay(ELISIA), and the expression of apoptosis in cartilage tissue detected by TUNEL assay,the levels of bcl-2 and bax detected by immunohistochemistry in cartilage tissue. Result: Compared with model group, the indexes showed significant improvement in rabbits of Yilong Tongbi decoction and glucosamine hydrochloride capsules group like the ultrastructure changes of arthrodial cartilage and synovium, levels of IL-1β and TNF-α in synovial fluid, cartilage apoptosis rate, the expression of Bcl-2, Bax and Bcl-2/Bax in cartilage tissue. Conclusion: Yilong Tongbi decoction can decrease the levels of IL-1β and TNF-α in synovial fluid, lessen the rate of chondrocyte apoptosis, improve the rate of Bcl-2/Bax,so as to protect cartilaginous tissue in the rabbit with knee osteoarthritis.关键词：Yilong Tongbi decoction;osteoarthritis;chondrocyte apoptosis;chondrocyte apoptosis regulatory genes
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发布时间：2024-01-04 - 摘要：Objective:To study the effect of Huogu Dan capsule on hemorheology, blood lipids, blood calcium and bone metabolism indicators of avascular necrosis of femoral head by hormone in rats. Method: Sixty SD rats were divided into 6 groups,the control group, the model group, the high, medium, low-dose group (1.80, 0.90, 0.45 g·kg-1), the positive drug group(0.28 g·kg-1). Except the normal group, each group was im given once a week prednisolone (24.5 mg·kg-1). Meanwhile each dose group was ig Huogu dan capsule.After 8 weeks rats were sacrificed to collect blood from carotid artery for determination of the whole blood viscosity, plasma viscosity, ESR, the serum total cholesterol(CHO), triglycende(TG), alkaline phosphatase(ALP), acid phosphatase(ACP), serum calcium(Ca) and phosphorus(P). Result: Compared with the control group, the whole blood viscosity in the model group was significantly increased (P<0.01). ESR values and plasma viscosity were significantly increased (P<0.01). CHO, TG and ALP levels were significantly increased (P<0.01). Ca level was increased (P<0.05).Compared with model group, Ca and P levels were significantly increased in the Huogu dan group, ALP and ACP were reduced, whole blood viscosity, plasma viscosity and blood lipids were reduced, statistical significance is different (P<0.05,P<0.01). Conclusion: The Huogu Dan capsule can be significantly increased Ca and P levels, and reduce ALP,ACP levels,whole blood viscosity, plasma viscosity and blood lipids.关键词：glucocorticoid-induced avascular necrosis of femoral head;Huogu Dan capsule;hemorheology
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发布时间：2024-01-04 - 摘要：Objective:To research the effect of Wendan decoction[It was made up of Pinelliae Rhizoma, Poria, Bambusae Caulis in Taenias, Aurantii Fructus Immaturus, Citri Reticulatae Pericarpium, Glycyrrhizae Radix et Rhizoma Prasparata Cum Melle.]on superoxide dismutase(SOD), malondialdehyde(MDA), nitric oxide(NO) and protein kinase(PKC) in rats serum with schizophrenia. Method:Fifty SD rats were randomly divided five groups: normal group(A), model group(B), Wendan decoction high-dosage group(C), Wendan decoction medium-dosage group(D) and Wendan decoction low-dosage group(E), ten rats in each group. Before established the model, the C, D, E groups were given Wendan decoction (40, 20, 10 g·kg-1) and the A and B groups were given normal saline every day, which last for 21 days. After two hours at the last given Wendan decoction or saline, the B, C, D, E groups were ip given MK801 0.6 mg·kg-1 to establish rat schizophrenia the model at the left side of the rat abdominal cavity. After general condition of rats was observed three days, the rats were sacrificed and serum was tested. The superoxide dismutase(SOD), malondialdehyde(MDA), nitri oxide(NO) in rats serum were detected by biochemical methods and the PKC in rats serum was detected by double antibody clip ELASA method. Result:Compared with B group, general condition of rats was improved, the MDA, NO, PKC content of group C, D and E were significantly decreased (P <0.05 or P <0.01) and their SOD was significantly increased (P <0.05 or P <0.01), pathological damage caused by oxygen free radical was alleviated. The PKC content of group C, D and E were significantly decreased (P <0.05 or P <0.01). Conclusion:Wendan decoction could reduce the pathologic lesions of rat with schizophrenia, protect from the damage caused by oxygen free radicals; Wendan decoction could decrease PKC content and thus play a regulatory role in the function of GJIC.关键词：Wendan decoction;schizophrenia;gap junction intercellular communication;protein kinase C
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发布时间：2024-01-04 - 摘要：Objective:To explore the effects of total flavones from Cuscuta chinensis (TFCC) on gonadal hormone in animal model of ovulation failure. Method: Animal model of ovulation failure was induced by intragastric administration of hydroxy urea, intragastric administration of TFCC was applied after establishment of ovulation failure; clomifene citrate was applied as positive control. Serum levels of FSH, LH, E2 and P were observed in control group (C), model group (M), TFCC low dose group (CCLD), TFCC high dose group (CCHD) and positive control group (PC) by radioimmunoassay method. Result: Compared with C, FSH, LH, E2 and P decreased significantly in M (P<0.05). Compared with M, serum levels of FSH, LH, E2 and P increased significantly in CCLD, CCHD and PC groups (P<0.05), more significantly in CCHD and PC groups (P<0.05). Conclusion: TFCC shows significant effect in improving recovery of gondadal hormone level in rat with ovulation failure, more significant when applied at high dose.关键词：total flavones from Cuscuta chinensis;ovulation failure;gonadal hormone
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发布时间：2024-01-04 - 摘要：Objective:This study evaluated the hepatoprotective activity of different molecule weight range of Dicliptera chinensis polysaccharide (DCP) against D-galactosamine (D-GlaN)-induced hepatotoxicity in SD rats. Method: DCP was divided into three parts according to the semipermeable membrane with different molecular weight cutoff (MWCO) using dialysis. Mw<6 kD, Mw 6 kD-10 kD, Mw>10 kD and DCP were studied on D-GlaN-induced (500 mg·kg-1 intraperitoneally) hepatic damage in rats. Liver coefficient, serum enzyms as alanine aminotransferase(ALT), aspartate aminotransferase(AST), hepatic tissue index malondialdehyde (MDA) and glutathione peroxidase (GSH-Px), and histopathological Changes in liver architecture including necrosis and swelling of hepatic cells were detected. Result: Compared with the model group, pretreated with DCP and Mw<6 kD polysaccharide significantly prevented the increases of alanine ALT and AST level in serum, each group can prevented MDA accumulation, GSH-Px depletion in liver tissue, and hepatocyte swelling and necrosis. Conclusion: The results indicate that DCP can protect against D-GlaN-induced acute liver injury in rats, and the most active part is the range of molecular weight left than 6 kD.关键词：Dicliptera chinensis polysaccharide;D-galactosamine;acute liver injury
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发布时间：2024-01-04 - 摘要：Objective:Study the way to make Huizhuo Tanzu type atherosclerosis animal model. Method: Divide 60 ApoE gene knock out mouse into 4 groups: high fat diet and cold dry environment intervention animal model group, high fat diet control group, cold dry environment intervention control group and normal control group. 12 weeks after nurturing in different groups, the weight, emotion, defecation, tongue presentations were observed in all animals.Biochemical indicators and cell morphology were compared between groups after sacrificing all the animals. Result: Indicators of Huizhuo Tanzu type atherosclerosis in High fat diet and cold dry environment intervention animal model group animals are significantly higher than other groups (P<0.05) . Light microscopic observations revealed that all the animals developed atheromatous plaque. high fat diet and cold dry environment intervention alone has significantly different affect on the development of atheromatous plaque comparing to a combined intervention of both high fat diet and cold dry environment (ANOVA , P<0.05). Conclusion: Animal underwent combined intervention of high fat diet and cold dry environment significant biological characteristics of Huizhuo Tanzu type atherosclerosis, indicating that animal models were successfully made.关键词：atherosclerosis;Huizhuo Tanzu type;animal models
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发布时间：2024-01-04 - 摘要：Objective:To explore the effect of Erzhi pills on expressions of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9(MMP-9) in brest cancer induced by 7,12-dimethylbenz(a) anthrancene (DMBA) in rats. Method: Sixty female SD rats were randomly divided into 6 groups:the blank control group,the DMBA model group, the high, middle, low dose (6.4,3.2,1.6 g·kg-1, respectively) Erzhi Pills group and tamoxifen (TAM) group. All model rats were induced by DMBA (100 mg·kg-1, twice, every two weeks), palpated weekly to monitor the progress of the tumor and body weight were also recorded weekly. At the end of trial, the incidence of breast tumors in each group was calculated. And the expressions of VEGF and MMP-9 in breast cancer tissues were detected using immunohistochemical SP method. Result: Comparing with the DMBA model group, the breast cancer incidence, number and volume of tumor in the middle dose of Erzhi pills group and TAM group decreased significantly(P＜0.05). The strong-positive rates of VEGF and MMP-9 were significantly higher in the group treated with middle and high dose of Erzhi pills than DMBA model group(P＜0.05), and VEGF expression was positively correlated with MMP-9 expression in breast cancer. Conclusion: Erzhi pills might inhibit the progress and deterioration of breast cancer through regulating the expressions of VEGF and MMP-9.关键词：breast cancer;DMBA;VEGF;MMP-9
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Huiru Yizeng Ⅰ on hyperplasia of mammary glands in rats, and explore the mechanism. Method: Eighty rats were divided into control group,disease model group,integrated disease model group,tamoxifen group(1.57 mg·kg-1),Rupi Sanjie group(0.50 g·kg-1),Huiru Yizeng Ⅰ high(31.6 g·kg-1),middle(15.80 g·kg-1)and low dose(7.9 g·kg-1)group. The model of mammary gland hyperplasia was induced by im benzoate estradiol and progesterone. The model of integrated disease was made by cliping the tail of every rat with a tong after experiencing mammary gland hyperplasia. The model rats were treated with different drugs for 4 weeks respectively. The breast diameter, body weight and serum hormone(determined by ELISA)was determine and pathomorphology change of glandular tissue in all groups was detected by microscope. Result: Huiru Yizeng Ⅰ could significantly diminish the breast diameter(P<0.01)and restore the body weight of rats(P<0.01), decrease serum estradiol(E2) [(2.387±0.22) pmol·L-1,P<0.01], prolactin (PRL)[(300.17±17.26) ng·L-1,P<0.05], follicle-stimulating hormone(FSH) [(0.42±0.019) U·L-1,P<0.05)], gonadotropin releasing hormone(GnRH) [(1.175±0.35) ng·L-1,P<0.01] and 5-hydroxy tryptamine(5-HT) [(18.179±2.053)ng·L-1,P<0.05], increase progesterone (P) [(0.99±0.078) μg·L-1,P<0.05], luteinizing hormone(LH) [(1.724±0.254) U·L-1,P<0.01), and significantly lessen the amount of lobules of mammary gland, gland alveolus and secretion. Conclusion: Huiru Yizeng Ⅰ can remarkably improve the disorder of serum hormone, and decrease the occurrence of hyperplasia of mammary glands.关键词：Huiru Yizeng Ⅰ;hyperplasia of mammary glands;integrated disease model;hormone;pathomorphology
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发布时间：2024-01-04 - 摘要：Objective:To study the effects of Rubia cordifolia L. and Charred R. cordifolia on coagulation-fibrinolytic system in normal Wistar rats and their mechanism of stasis-resolving hemostatic based on their pharmacological action. Method: Wistar rats were randomly divided into blank control group(normal saline,10 mL·kg-1), R. cordifolia low-dose (5.0 g·kg-1) and high-dose (10.0 g·kg-1) groups, Charred R. cordifolia low-dose (5.0 g·kg-1) and high-dose (10.0 g·kg-1) groups and positive control group p-aminomethylbenzoic acid,(0.6 g·kg-1) ig,one time a day for 7 days. The blood in abdominal aorta was taken and then the plasma was separated in the eighth day. The activities of plasma tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor (PAI) and plasminogen (PLG) in plasma were measured by ELISA method. Result: Compared with the blank control group, both the low-dose and high-dose group of R. cordifolia can increase t-PA activity, the high-dose group of R. cordifolia can increase t-PA activity more obviously (P＜0.01). The high-dose group of Charred Rubia cordifolia L. can also increase t-PA activity (P＜0.05) but the low-dose group of Charred Rubia cordifolia L. cannot. In addition, the high-dose group of Charred R.cordifolia's effect on t-PA activity is smaller than the high-dose group of R. cordifolia The effects of R. cordifolia and Charred R. cordifolia on the PAI and PLG activity are all not significant regardless of their dosage. Conclusion: Both the low-dose and the high-dose group of R. cordifolia can remarkably affect fibrinolytic system to play its role on stasis-resolving hemostatic through increasing t-PA activity. Charred R. cordifolia can also affect fibrinolytic system while it may be the comprehensive effects of many enzymes. R. cordifolia and Charred R. cordifolia have different pharmacological effects on fibrinolytic system. Thereby, they have different uses in clinical practice.关键词：R. cordifolia;Charred R. cordifolia;t-PA;PAI;PLG;coagulation-fibrinolytic system
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of total alkaloids from Phyllodium pulchellum on proliferation of human hepatic stellate cell line Lx-2 and the impact on secretion of collagen and cytokines which are related to liver fibrosis. Method: After recovery, Lx-2 cells in logarithmic growth were diluted to a concentration of 1×105/mL,and inoculated 0.1 mL in hole in incubator at 37 ℃, blank control group was set up, the concentration of the drug administered in an order of 100,75,55,40,30,20,15,10 mg·L-1. After 48 h of administration, MTT assay was used to detect cell proliferation. Similarly, after recovery, Lx-2 cells in logarithmic growth were diluted to a concentration of 1×105/mL,and inoculated 0.1 mL/hole in incubator at 37 ℃, blank control group and Colchicine group (6.25 mg·L-1) were set up. After 48 h of administration, ELISA method was used to detect Lx-2 cell supernatant for type I collagen (COL I), type III collagen (COL III), type IV collagen (COL Ⅳ), transforming growth factor β1(TGF β1) and platelet-derived growth factor (PDGF) content. Result: Compared with the control group, Lx-2 cell proliferation was inhibited by the total alkaloids in a concentration-dependent manner with inhibition rates of 49.4%, 25.9%, 24.7%, 15.3%,15.3%,12.9%,2.35% and 1.18% accordingly. Compared with the control group, the total alkaloids of 40 mg·L-1 could significantly inhibit COL I, COL Ⅲ, COL Ⅳ, TGF-β1 and PDGF secretion ( P<0.05 or P <0.01). Conclusion: The total alkaloids from P. pulchellum can inhibit the proliferation of Lx-2 cells and the secretion of COLⅠ, COL III, COL IV,TGF-β1and PDGF.关键词：Lx-2;total alkaloids from Phyllodium pulchellum;proliferation;collagen;cytokines
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发布时间：2024-01-04 - 摘要：Objective:To compare the pharmacological effects between the powdered Xuanfei Huatan decoction (PXHD) and the ordinary decoction (OXHD) of the same prescription. Method: A variety of experimental models was used, and rats, mice and guinea pigs were involved in the study. Rats were divided into eight groups. The rats in different groups were given distilled water,JI-ZHI syrup, 3 doses of DXHD (46.80,23.40, 11.70 g·kg-1) and 3 doses of PXHD (23.40, 11.70,5.85 g·kg-1) respectively. The pharmacodynamic comparison between SXHD and OXHD included the follows:ammonia-induced cough in mice, phosphorylation histamine-induced asthma in guinea pigs, tracheal secretion of phenol red in mice, capillary permeability in mice and superoside dismutas(SOD) and malondialdehyde(MDA) in rats with chronic bronchitis. Result: PXHD and OXHD both could prolong the incubation period and reduce the cough frequency in mice;extend the latent period of asthma in guinea pigs;increase the amount of tracheal secretion of phenol red;reduce capillary permeability,and increase SOD and reduce MDA in rat with chronic bronchitis. No significant difference was found between the according level of PXHD and OXHD. Conclusion: Both PXHD and OXHD have the ability of suppressing cough, asthma and phlegm, and the ability of anti-inflammatory and antioxidant.关键词：Xuanfei Huatan prescription;prepared Chinese herbal medicine;powdered herbal decoction;chronic bronchitis;efficacy
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发布时间：2024-01-04 - 摘要：Objective:To investigate the anti-inflammatory activity of total flavonoids from Syneilesis aconitifolia. Method: Mice were involved in the study. The auricle edema was induced by dimethylbenzene, the abdominal cavity capillary permeability was increased by acetic acid, the granuloma was induced with cotton pellet, and the acute inflammation was induced by air-pouch. The anti-inflammatory effect of total flavonoids in different models were observed. Mice were divided into five groups, total flavonoids from S. acontifolia high dose, middle dose and low dose (400,200, 100 mg·kg-1) groups, dexamethasone (2.0 mg·kg-1) group and the model group (normal saline). The drug treatment lasted 7 days. Result: The total flavonoids from S. acontifolia showed significant inhibitory action on auricle edema induced by dimethylbenzene in mice (P<0.01), the inhibitory rate was 51.55%. The total flavonoids from S. acontifolia showed significant inhibitory action on penetration of capillary vessel in mice (P<0.01). The flavonoids also decreased the proliferation of granuloma caused by implantation cotton pellet in mice significantly. The amount of WBC and content of prostaglanding E2(PGE2) in the exudates in experimental groups were obviously lower than those in the control group (P<0.01). Conclusion: The total flavonoids from S. aconitifolia have significant anti-inflammation action, which is likely related to the decrease in capillary permeability and inhibition of production and release of the inflammatory factor PGE2.关键词：Syneilesis aconitifolia;total flavonoids;anti-inflammatory;granuloma;PGE2
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发布时间：2024-01-04 - 摘要：Objective:To probe into the relationship between combined medication and safety of Danhong injection, and offer a reference for clinical medication. Method: Multi-center and large sample hospital centralized monitoring method was applied to study the combined medication among 10 409 inpatients who used Danhong injection. The related data were mined and analyzed. Result: Among all inpatients investigated, the rate of combined use of Danhong injection and chemical drugs was 99.7%. Drugs frequently combined with Danhong injection were cardiovascular and cerebrovascular medicine, psychotherapeutic drug, enteral and parenteral nutrition and regulation water, electrolyte, acid-base balance drug and endocrine drug. The adverse reactions of Danhong injection positive correlated with stomatological preparation, anti-rheumatic and orthopaedic drug, enteral and parenteral nutrition and regulation water, electrolyte, acid-base balance drug and dermatologic drugs. Conclusion: Adverse reactions of Danhong injection related to the types and quantity of combined drugs. Safety monitoring of Danhong injection should be enhanced when it is combined with other drugs.关键词：combined with medication;Danhong injection;safety
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发布时间：2024-01-04 - 摘要：Objective:To observe the influence Bushen Tongluo fang on diabetic peripheral neuropathy (DPN) oxidative stress. Method: Fourty-eight DPN patients were randomly divided into 2 groups, 30 cases in treatment group and 18 cases in control group, two groups were given conventional treatment and lipoic acid for 90 days, in the same time the treatment group were given Bushen Tongluo fang. The clinical curative effect and changes of blood sugar,haemoglobin A1c (HbA1c),triglyceride (TG), total cholesterol(CHOL), nerve conduction velocity, malondialdehyde (MDA), superoxide dismutase (SOD) were observed. Result: After treatment, the total effective rate of treatment group was 83.3%, and the placebo group was 66.7%. Between the two groups, the difference was significant (P<0.05). The levels of fasting blood glucose(FBG), HbA1c, TG, CHOL in two groups were significant declined than before (P<0.05), and TG, CHOL declined was better in treatment group than control group (P<0.05). After treatment, motor nerve conduction velocity(MNCV), sensory nerve conduction velocity(SNCV) in two groups were obviously improved than before (P<0.01, P<0.05), and the improvement in treatment group was better than control group (P<0.01). After treatment, MDA was markedly reduced (P<0.01) and SOD obviously improved (P<0.01, P<0.05) in two groups, and the effect in treatment group was better than control group (P<0.01). Conclusion: Bushen Tongluo fang on the basis of conventional treatment and lipoic acid in the treatment of DPN patients can improve the clinical curative effect, improve lipid metabolism, reduce oxidative stress reaction and improve nerve conduction velocity.关键词：diabetic peripheral neuropathy;oxidative stress;Bushen Tongluo fang
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发布时间：2024-01-04 - 摘要：Objective:To observe the effect of heart and kidney-reinforcing formula related indicators and the plasma brain natriuretic peptide (BNP) of in chronic heart failure patients. Method: Forty cases with chronic heart failure patients based on double blind method were randomly divided into two groups. The two groups were given the same basic treatment, including low salt diet, bed rest, when necessary, the condition of oral inhalation and routine western medicine (ACEI, diuretics, vasodilators, digitalis, beta blockers) were carried out. The treatment group was given the heart-kidney treatment of cardiac double compensation, boiling juice 150 mL, 2 times a day,course of treatment lasted8 weeks.The following indexes were observed and record ed before and after treatment,symptoms, tongue, pulse and cardiac ultrasound index, left ventricular end diastolic diameter(LVEDD), end systolic diameter(LVESD), ejection fraction (EF), determination of plasma BNP, endothelin (ET) and nitric oxide content. And recorded before and after the treatment of safety indexes, such as blood pressure, electrocardiogram, liver and kidney function, blood rheology, blood, urine routine. A detailed record of drug adverse reactions. Result: The plasma ET, LVEDD, LVESD, NO levels were significantly reduced compared with before treatment (P<0.05), BNP levels were significantly reduced (P<0.01), ejection fraction EF were increased significantly (P<0.01). Conclusion: The heart and kidney-reinforcing formula is effective and safe.关键词：chronic heart failure;Xinshen Shuangbu Qiangxin decoction;heart function;plasma BNP
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发布时间：2024-01-04 - 摘要：Objective:To explore the clinical effects of Buyang Huanwu decoction combined with Edaravone injection in treating patient with acute ischemic stroke. Method: All patients were randomLy divided into two groups:treatment group with 48 cases and control group with 48 cases. The control group was given Edaravone injection 30 mg twice a day,and the treatment group was received additional Buyang Huanwu decoction based on the control group once a day.The course of treatment was 15 days. The results were compared with clinical effects,national institute of health stroke scale (NIHSS),activities of daily living(ADL,Barthel Index) and traditional Chinese medicial(TCM) symptom accumulated points. Result: The total effective rate in treatment group and control group was 95.83% and 77.08%, respectively, the cured and markedly effective rate was 81.25% and 47.92%, respectively.In treatment group the total effective rate and cured and markedly effective rate were significantly higher than that in control group. After the treatment, the score NIHSS,ADL and TCM symptom accumulated points in treatment group were significantly better than that in control group.There were significant differences between two groups in the total effective rate, cured and markedly effective rate,NIHSS,ADL and TCM symptom accumulated points (all P<0.05). Conclusion: Buyang Huanwu decoction combined with Edaravone injection in treating patient with acute ischemic stroke has definitely therapeutic effect.关键词：acute ischemic stroke;Edaravone injection;Buyang Huanwu Decoction
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of Qishen Yiqi dripping pills on heart function and high-sensitivity C-reactive protein(hs-CRP)and B-type brain natriuretic peptide (BNP) levels in the patients with chronic heart failure. Method: One hundred and twenty CHF patients were divided into routine therapy group and Qishen Yiqi dripping pills group.Left ventricular ejection fraction(LVEF), left ventriculay end systolic diameter(LVESD)and left ventrieular end diastolic diameter (LVEDD) before and after treatment were detected.Serum hs-CRP and BNP were measured by rapid fluorescence immunoassay. Result: LVEDD and LVESD were shortened,LVEF was improved in Qishen Yiqi dripping pills group(P<0.05).Concentrations of serum hs-CRP and BNP levels were decreased in Qishen Yiqi dripping pills group after treatment(P<0.01). Conclusion: The heart function is further improved,concentrations of serum hs-CRP and BNP levels are further decreased by treated with Qisben Yiqi dripping pills in heart failure patients.关键词：chronic heart failure;Qishen Yiqi Dripping pills;high-sensitivity C-reactive protein;brain natriuretic peptide
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发布时间：2024-01-04 - 摘要：Objective:To study the therapeutic effect of Shenling Baizhu powder on chronic otitis media with effusion (OME) in a spleen deficiency type. Method: Sixty patients of OME in a spleen deficiency type were included in the trial.The patients were randomLy divided into experimental group,drug treating group,and 30 healthy people were included as control group.After one course of treatment (14 days),to test the serum concentration of interleukin(IL)-4,IL-6 and interferon-γ(IFN-γ) of each group by ELISA,and to compare the clinical efficacy of each group. Result: ①experimental group had a better therapeutic effect than drug treating group(P<0.05);②compared to control group,both experimental group and drug treating group had lower serum concentration of IFN-γ and higher concentration of IL-4,IL-6(P<0.01);③after one course of treatment(14 days),the serum concentration of IL-4,IL-6 had been downregulated,IFN-γ had been upregulated,experimental group was surperior to drug treating group. Conclusion: Shenling Baizhu powder had a significantly clinical efficacy on chronic otitis media with effusion in a spleen deficiency type,and can media the immune response,so it is worthy to promote it being used widely.
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发布时间：2024-01-04 - 摘要：Objective:To investigate the clinical efficacy of Jieyu Ganshu capsules (JGC) plus adefovir dipivoxil tablets and bifendate tablets for treating patients with chronic hepatitis B (CHB) and the effect on immunoregulatory function. Method: One hundred and three patients (liver-stagnation and spleen-deficiency syndrome) were randomly divided into observation group (n=52) and control group (n=51). Control group was given adefovir dipivoxil tablets, 10 mg daily;bifendate tablets, 25-50 mg each time, tid, and withdrawal after 4 weeks for liver function was normal. JGC was added to observation group, 3 capsules each time, tid. All patients were received treatment for after 24 weeks accordingly. Hepatitis be antigen (HbeAg), alanine aminotransferase (ALT), hepatitis B virus-DNA (HBV-DNA), T cell subset and serum immunoglobulin were tested, and score of liver-stagnation and spleen-deficiency syndrome was observed. Result: The level of HBV-DNA and ALT in both groups after the treatment were lower than those before, and the post-treatment HBV-DNA level of observation group was more lower compared with that in control group after 24 weeks (P<0.01). The post-treatment level of ALT of observation group was lower than those in control group after 12, 24 weeks (P<0.01). The post-treatment complex rule number of ALT of observation group were superior to those in control group after 12, 24 weeks (P<0.05). The post-treatment HBV-DNA negative number in observation group was superior compared with that in control group (P<0.05). CD3+, CD4+ and CD4+/CD8+ in observation group were increased obviously and higher than those in control group, and CD8+ was dramatic declined and lower than that in control group(P<0.05, P<0.01). The level of IgG, IgA and IgM in observation group were declined obviously and lower than those in control group (P<0.01). Bloating, distending pain in hypochondrium, abdominal distension, poor appetite and indigestion, depressed mood, preference for sighing, syndrome score of liver-stagnation and spleen-deficiency in observation group after treatment were lower compared with those in control group (P<0.01). Conclusion: JGC plus adefovir dipivoxil tablets and bifendate tabletes can resist HBV, protect liver function, relieve the clinical symptoms, and the mechanism of action may be related with regulating cellular immunity and humoral immunity function.关键词：chronic hepatitis B;Jieyu Ganshu capsules;adefovir dipivoxil tablets;bifendate tablets;T cell subset;immune globulin
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of maintenance treatment by traditional Chinese medicine (TCM) syndrome differentiation on life quality and progress-free survival (PFS) of patients with advanced non-small cell lung cancer (NSCLC) treated with chemotherapy. Method: Seventy-six patients with NSCLC were randomly divided into observation group and control group (n=39 each). The two groups were treated with first-line chemotherapy for 4 to 6 weeks. The control group was regularly followed-up. The observation group was received TCM syndrome differentiation treatment. Follow-up data about PFS was analyzed, besides, life quality and clinic symptom of patients before and 12 weeks after treatment were evaluated. Result: After treatment, physical function, role function, emotional function and total life quality in observation group were higher than those in control group (P<0.05). Fatigue, dyspnoea, insomnia and appetite loss scores in observation group were lower than those in control group (P<0.05). PFS of observation group was (7.2±1.32) months, which was longer than (4.4±0.57) months of control group (P<0.01). Scores of cough, phlegm, blood in phlegm, shortness of breath, chest pain, lassitude, anorexia, insomnia and others in observation group were lower than those in control group (P<0.01). Conclusion: Maintenance treatment by TCM syndrome differentiation on patients with advanced NSCLC can improve life quality and clinic symptom, and prolong PFS of patients.关键词：non-small cell lung cancer;maintenance treatment;TCM therapy;life quality;progress-free survival
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发布时间：2024-01-04 - 摘要：Objective:To observe the impact of the Huxin formula therapy in ventricular remodeling and heart function of the patients with acute myocardial infarction. Method: Sixty-six patients were randomly divided into observation group and control group. Patients in two groups were both given conventional treatment. Huxin formula orally for 8 weeks) was given to the patients in the observation group. Echocardiography was used to check the heart function before and after treatment. Angiotensin II (Ang II), transforming growth factor β1 (TGF β1), vascular superoxide dismutase (SOD), serum procollagen IIIN-terminal propeptide (PIIINP) and aldosterone (ALD) levels were detected and Qi stagnancy and blood stasis syndrome score was also observed. Result: Left ventricular end-diastolic diameter (LVDd), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVSDV), cardiac index (CI) and left ventricular ejection (LVEF) of patients in the observation group were significant improved after treatment (P<0.05,P<0.01), and improvement of LVEDV and LVEF were better than the patients in the control group (P<0.05). AngⅡ, PⅢNP, ALD and TGF β1 of the patients in the observation group were lower than patients in the control group (P<0.05,P<0.01), SOD level of the patients in observation group was higher than the patients in control group, scores of precordial pain, shortness of breath, palpitation, chest tightness, irritability and Qi stagnancy and blood stasis in observation group was lower than those in the control group. Conclusion: Huxin formula therapy can prevent ventricular remodeling after myocardial infarction, it can also improve clinical symptoms and heart function.关键词：acute myocardial infarction;ventricular remodeling;Huxin formula;heart function
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发布时间：2024-01-04 - 摘要：Objective:To observe the treatment effects and safety of Bushen decoction with moxibustion of temperature sensitive acupoint on sterility due to anovulation, and to explore the mechanism of action of the treatment in curing sterility. Method: Sixty patients with sterility due to anovulation were randomly divided into two groups, the treatment group (30 cases) took the Bushen decoction with moxibustion of temperature sensitive acupoint, while the control group (30 cases) took Bushen decoction only. They were continuously treated for three months, and then tested basal body temperature (BBT), and using trans-vaginal ultrasound to test the follicle diameter and thickness of uterine endometriums, and made detection of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol2 (E2), prolactin (PRL), testosterone (T) in serum. The pregnancy rate within 1 year was also investigated. Result: An efficiency of 90% in the treatment group was significantly better than 60% in the control group (P<0.05). The total efficiency of traditional Chinese medicine syndromes of the treatment group was 70% better than 43.33% of the control group ( P<0.01). The pregnancy rate of the treatment group was 63.33% better than 26.67% of the control group ( P<0.05). In treatment group the follicle diameter and endometrial thickness were increased and better than those of the control group( P<0.01). BBT recovery rate was 70% in treatment group better than 33.33% in control group ( P<0.01). FSH, LH and E2 of the treatment group were significantly increased more than those in the control group ( P<0.05). Conclusion: The therapy of Bushen decoction with moxibustion of temperature sensitive can improve anovulatory infertility with regulated hormone levels, prompting BBT recovery, improving clinical symptoms, and increasing pregnancy rate.关键词：kidney asthenia;sterility due to anovulation;Bushen decoction;moxibustion of temperature sensitive acupoint
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发布时间：2024-01-04 - 摘要：Objective:To investigate the clinical efficacy of combination of Chinese traditional and western medicine on ectopic pregnancy. Method: Ninety-six subjects were randomly divided into observation group and control group (n=48 each). Control group was given methotrexate (MTX) 50 mg·m-2 by intramuscular injection, once. Observation group was received Shaofu Zhuyu decoction on the basis of control group, and the prescription was adjusted according to the level of human chorionic gonadotrophin (β-HCG), 1 dose daily. The decoction was prepared daily and taken 2 times (half by half), for 4 weeks. Vital signs and serum β-HCG level were monitored, the extrauterine pregnancy package of block was monitored by B ultrasonic, and tubal patency tests were applied. Result: The success rate of observation group (91.66%) was superior to that in control group (72.92%) (P<0.05). The recovery duration of β-HCG, the duration of abdominal pain disappearance and the duration for absorption of uterine rectal pouch effusion in the observation group were less than those in the control group (P<0.05). The enclosed mass in the observation group after treatment was less than that in control group (P<0.01). Tubal patency rate of observation group (89.65%) was superior to that in control group (70.37%) (P<0.05). Conclusion: The conservative treatment of combination of Chinese traditional and western medicine for ectopic pregnancy could kill fetus, promote quick absorption of enclosed mass, maintain tubal patency, preserve the fertility. It is safe and worthy for being widely used in clinical treatment.关键词：ectopic pregnancy;conservative treatment;Methotrexate;Shaofu Zhuyu Decoction;combination of Chinese traditional and western medicine
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发布时间：2024-01-04 - 摘要：Objective:To observe the efficacy of herbal fumigation and topical on the early closed fracture. Method: One hundred and twenty subjects were randomly divided into observation group and control group(n=60 each). Both groups were carried out conventional reset and set and were given lamiophlomis rotata capsule, 3 capsules tid for 4 weeks. Control group was given local physical therapy, and observation group were given herbal fumigation, 3 times one day, for 7 days, and herbal topicals, 1 time daily, for 4 weeks. Pain, tenderness, ecchymosis, dysfunction and swelling were evaluated before and after treatment for 7 days, and adverse reactions and complications were observed. Result: The scores of pain, tenderness, ecchymosis, dysfunction and swelling in the observation group were lower than those in control group (P<0.01). The total effective rate of observation group (100%) was superior to that in the control group (88.3%) (P<0.05). The occurrence rate of tension vesicle in the observation group was less than that in control group (P<0.05). Conclusion: The application of herbal fumigation and topicals for the treatment of early closed fracture can significantly improve early pain, dysfunction and swelling, and it can enhance clinical effects and reduce complications关键词：closed fracture;herbal fumigation;herbal topicals;clinical effects
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of zhipiantong capsule for treatment of migraine and its influence on plasma 5-hydroxy tryptamine(5-HT). Method: One hundred and twenty-four patients with migraine were randomly divided into observation group and control group (n=62 each). Control group was orally received flunarizine capsules 5 mg at night daily. Observation group was orally received Zhipiantong capsules, 4 capsules, tid. If patients feel unbearable pain during treatment, they were orally received ibuprofen tablets, 0.1 g. The treatment course of two groups was 8 weeks. Frequencies, days, extent and associated symptoms of migraine were observed. Blood flow velocity was determined by transcranial Doppler (TCD), and plasma 5-HT was tested. Result: The total curative effective rate of observation group (90.32%) was higher than that of control group (77.41%) with a significant difference (P<0.05). The total syndrome effective rate of observation group (91.93%) was higher than that of control group (75.81%) with a significant difference (P<0.05). After treatment, frequencies, days and extent of migraine in observation group were lower than those in control group (P<0.01). Plasma 5-HT level in observation group was higher than that in control group (P<0.01). The dosage of analgesic drugs used in observation group was less than those in control group during treatment. Blood flow velocity in middle cerebral artery(MCA), anterior cerebral artery(ACA), posterior cerebral artery(PCA), intracranial segment of the vertebral artery(VA) and basilar artery (BA) in the observation group was slower than those in control group (P<0.01). Conclusion: Zhipiantong capsule can decrease the mean blood flow velocity in cerebral arteries, improve migraine clinical symptoms and treat migraine effectively, the mechanism of which may be related to increasing plasma 5-HT.关键词：migraine;Zhipiantong capsule;transcranial Doppler;5-HT
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发布时间：2024-01-04 - 摘要：Objective:To investigate the treatment based on differentiation of symtoms and sigins of traditional Chinese medicine(TCM) in the treatment of osteonecrosis of the femoral head. Method: Patients with necrosis of the femoral head in Qi stagnation and blood stasis,deficiency of liver and kidney and heat resistance were enrolled(30 cases each). According to symptom differentiation treatment of 3 months was 1 courses. After 3 courses , Harris hip score was compared. Result: The deficiency of liver and kidney in patients with femoral head necrosis rate(83.33%)was significantly higher than that of qi stagnation and blood stasis (60%) and heat resistance (56.67%)(P<0.05).After the treatment of Harris hip score in 3 groups showed difference cmpared with that before treatment(P<0.01). After the treatment,Harris hip score liver and kidney deficiency type and liver and kidney deficiency type, heat resistance type showed difference(P<0.05). That in liver and kidney deficiency type was better than those of other two types. Conclusion: According to syndrome differentiation and disease differentiation, the principle of TCM syndrome differentiation treatment for osteonecrosis of the femoral head can improve the treatment effect.关键词：avascular necrosis of the femoral head;syndrome differentiation;treatment based on differentiation of symptoms and signs
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发布时间：2024-01-04 - 摘要：Objective:To investigate the effect of treatment of infant recurrent respiratory infection (RRTI) with combination of traditional Chinese medicine and Western medicine. Method: Ninety RRTI patients were randomly divided into observation group and control group (n=45 each). At attack stage, the two groups were received routine western medicine therapy. At remission stage, control group was orally received transfer factor oral solution, 10 mL once daily, and observation group was orally treated with Huangqi Shengmai yin, 10 mL twice daily and Shenlin Baizhu powder, 1 agent daily (taken half by half). The treatment course of two groups was 8 weeks, and follow-up time lasted 6 months. Score of lung and spleen deficiency syndrome was observed, T-cell subsets and immunoglobulin were tested before and after treatment. Result: The total effective rate of observation group (97.77%) was higher than that of control group (66.66%) with a significant difference (P<0.01). The improvement of CD3+,CD4+,CD8+ and CD4+/CD8+ in observation group were better than those in control group (P<0.05, P<0.01). After treatment, IgG and IgA in observation group were increased significantly than those in control group (P<0.01). The syndrome score of lung and spleen deficiency in observation group were significantly lower than those in control group (P<0.01). Conclusion: Western medicine therapy, used at attack stage, combined with Huangqi Shengmai yin and Shenlin Baizhu powder, used at remission stage can improve physical condition of infected children, promote patient immunity, reduce clinical symptom and treat infant recurrent respiratory infection effectively.关键词：infant recurrent respiratory infection;Huangqi Shengmai yin;Shenlin Baizhu powder;T-cell subsets;immunoglobulin
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发布时间：2024-01-04 - 摘要：Objective:To study the molecular mechanism of Maxing Shigan decoction in the treatment of H1N1 influenza A:by establishing molecular networks and comparing canonical pathways. Method: Target proteins of Maxing Shigan decoction and related genes of H1N1 influenza A were searched based on Pubchem and Gene databases on line. Molecular networks and canonical pathways comparison analyses were performed by Ingenuity pathway Analysis(IPA). Result: There were 186 targets proteins of Maxing Shigan decoction and 7 related genes of H1N1 influenza A. The molecular networks of Maxing Shigan decoction and H1N1 influenza A were multifunction. There were four cellular immune related pathways in all eight common pathways including:role of pattern recognition receptors in recognition of viruses, interlewkin 12(IL-12) signaling and production in macrophages, role of proten kinase(PKR) in interferon induction and antiviral response and Tumor necrosis factor receptor 1(TNFR1) signaling.Maxing Shigan decoction could regulate Toll-like receptor(TLR9), phosphoinostide 3 -kinase(PI3K), nucleotide-binding oligomerization domain 2(NOD2) and other molecules of cellular immune related pathways. Conclusion: Regulating many effective nodes of cellular immune related pathways maybe the way of Maxing Shigan decoction against H1N1 influenza A.关键词：Maxing Shigan decoction;H1N1 influenza A;Ingenuity Pathway Analysis (IPA);PubChem
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发布时间：2024-01-04 - 摘要：Objective:To evaluate the effectiveness and safety of Yanhuning combined with and ribavir in treatment of the hand-foot-mouth disease. Method: Pubmed,CENTRAL,CBM,CNKI,VIP and Wanfang database were searched for clinical trials.Two evaluators chose trials and evaluated quality, valid data were put in Meta-analysis.Result:Twenty-six randomized clinical trials(RCT) and three thousand five hundred and seventy-one patiences were included. From the results of Meta-analysis , the effectiveness of Yanhuning combined with ribavirin was superior than the control group in the overall efficiency [OR=5.72,95%CI(4.17,7.85)],defervescence time [MD=-1.16,95%CI(-1.46,-0.85)], simplex subsiding time [MD=-1.56,95%CI(-1.87,-1.25)],and ulcer subsiding time [MD=-1.11,95%CI(-1.47,-0.75)].Conclusion: It may have a certain effect on curing the hand foot mouth disease when Yanhuning combined with ribavirin. But we need more high-quality RCT to support our inference and make an exact conclusion for its efficacy.关键词：Yanhuning;ribavirin;hand-food-mouth disease;Meta-analysis
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发布时间：2024-01-04 - 摘要：Objective:To explore the therapeutic rules of Chinese medicine prescription with anti-cancer proprietary. Method: One hundred and twenty-two kinds of Chinese medicine used in the treatment of cancer were collected, andits prescription composition and function of attending were analyzed to sum up the prescription rules. Result: Chinese medicine prescriptions with tonifying qi medicine, promoting blood circulation to remove blood stasis drugs, heating antidote and line gas drug the application frequency were frequently used. Conclusion: The treatment principles of Chinese medicine prescription iused for cancer were mainly in promoting blood circulation to remove blood stasis and clearing heat, qi, phlegm, etc.关键词：cancer;proprietary Chinese medicines;prescription rule
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发布时间：2024-01-04 - 摘要：Objective:To clear the main diseases of Shengyang Yiwei decoction spectrum and clinical indications, will provide the basis for the next step of the animal experiment model, and provide the theoretical basis for guiding clinical. Method: Mining by using the method of documentary information, statistics and analysis the clinical literature and medical literature published from 2012 September to various domestic medicine periodicals. Result: The method of collection, screening and treatment are of Shengyang Yiwei decoction clinical literature of 96 references, including the number of cases in 909 cases, the types of diseases in digestive system disease, a total of 313 cases, accounting for 34.4%. The main clinical syndrome differentiation and indications for use of core indications:loose stool, eating less poor appetite, lassitude;the main indications:frequent urination, lusterless complexion, limb discomfort;the tongue:tongue pale moss white or fat have teeth marks;the pulse:pulse or ru. The use of modified drugs mainly compatibility, astringent, tonic, Qi dampness Xiaoshi drugs.关键词：Shengyang Yiwei decoction;literature information;data mining;correspondence of prescription and syndrome
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