最新刊期
卷 19 ， 期 9 ， 2013
- 摘要：Objective:To investigate influence of ultrafine grinding on stability of Qili powders.Method:Common powder and micropowder of Qili powders were placed in the high humidity,high temperature and lighting conditions separately,the moisture content,angle of repose,content of dracorhodin and in vitro dissolution were measured regularly.Result:Under the high humidity cindition,the moisture content of these two kinds of powders increaced,while the flowability and the content of dracorhodin droped significantly,dissolution of dracorhodin had no significant change.Under the high temperature condition,the moisture content of the common powder and the micropowder droped,the flowability increased while dissolution behavior of dracorhodin became bad,the content of dracorhodin in the common powder droped while there was no significant change in the micropowder.Under the lighting condition,the moisture content of these two kinds of powders increaced,while the flowability remained and the content of dracorhodin droped significantly,dissolution behavior of dracorhodin in the common powder became bad,but it kept stable in the micropowder.Conclusion:Compared with the common powder,in vitro dissolution stability of dracorhodin in the micropowder was worse under the high temperature,but the micropowder was more stable under the lighting condition.Change of each assessment index had no significant difference between the common powder and the micropowder under the remaining conditions.关键词：ultrafine grinding;Qili powder;high humidity;high temperature;illumination;stability
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发布时间：2024-09-23 - 摘要：Objective: To investigate influence of ultrafine grinding on stability of Qili powders. Method: Common powder and micropowder of Qili powders were placed in the high humidity,high temperature and lighting conditions separately,the moisture content,angle of repose,content of dracorhodin and in vitro dissolution were measured regularly. Result: Under the high humidity cindition,the moisture content of these two kinds of powders increaced,while the flowability and the content of dracorhodin droped significantly,dissolution of dracorhodin had no significant change.Under the high temperature condition,the moisture content of the common powder and the micropowder droped,the flowability increased while dissolution behavior of dracorhodin became bad,the content of dracorhodin in the common powder droped while there was no significant change in the micropowder.Under the lighting condition,the moisture content of these two kinds of powders increaced,while the flowability remained and the content of dracorhodin droped significantly,dissolution behavior of dracorhodin in the common powder became bad,but it kept stable in the micropowder. Conclusion: Compared with the common powder,in vitro dissolution stability of dracorhodin in the micropowder was worse under the high temperature,but the micropowder was more stable under the lighting condition.Change of each assessment index had no significant difference between the common powder and the micropowder under the remaining conditions.关键词：ultrafine grinding;Qili powder;high humidity;high temperature;illumination;stability
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发布时间：2024-09-23 - 摘要：Objective: To investigate effects of Guizhi decoction and its disassembled prescriptions on the content of paeoniflorin. Method: A gradient HPLC system was used in the quantitative analysis of paeoniflorin on Diamonsil RP-C18 column (4.6 mm×250 mm,5 μm) with acetonitrile and water as mobile phase,the flow rate was 1 mL·min-1and the detection wavelength was 230 nm. Result: The content of paeoniflorin had great changes comparing Guizhi decoction with its different disassembled prescriptions,the order of it was group 4>group 3>group 5>group 1>group 2;By comparing with group 5,group 2 and group 1 had significant differences(P<0.05),but group 3 and group 4 had not. Conclusion: Radix Ei Rhizoma Glycyrrhizae and Ramulus Cinnamomi were helpful to the dissolution of paeoniflorin from Radix Paeoniae Alba,but Rhizoma Zingiberis Recens and Fructus Jujubae had few effects on it.关键词：Guizhi decoction;disassembled prescriptions;paeoniflorin;determination of content;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To investigate effects of Guizhi decoction and its disassembled prescriptions on the content of paeoniflorin.Method:A gradient HPLC system was used in the quantitative analysis of paeoniflorin on Diamonsil RP-C18 column（4.6 mm×250 mm,5 μm） with acetonitrile and water as mobile phase,the flow rate was 1 mL·min-1and the detection wavelength was 230 nm.Result:The content of paeoniflorin had great changes comparing Guizhi decoction with its different disassembled prescriptions,the order of it was group 4>group 3>group 5>group 1>group 2;By comparing with group 5,group 2 and group 1 had significant differences（P<0.05）,but group 3 and group 4 had not.Conclusion:Radix Ei Rhizoma Glycyrrhizae and Ramulus Cinnamomi were helpful to the dissolution of paeoniflorin from Radix Paeoniae Alba,but Rhizoma Zingiberis Recens and Fructus Jujubae had few effects on it.关键词：Guizhi decoction;disassembled prescriptions;paeoniflorin;determination of content;HPLC
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发布时间：2024-09-23 - 摘要：Objective: To study correlation between boiling point and vacuum in vacuum water extraction technology,and compare effects of different medicinal dosage on the vacuum and the average molar heat of vaporization. Method: Theoretical calculation and decompression test determination were used to obtain the vacuum at different boiling point.Then the saturated vapor pressure value and the average molar heat of vaporization in this system were calculated. Result: There are changes between the measured and theoretical values of vacuum with the pure solvent;After added medicinal materials,the measured values of vacuum were lower than the measured values of the pure solvent,the measured values of the average molar heat of vaporization were lower than the values of the pure solvent.Effects of medicinal dosage on the measured value of vacuum had no significant. Conclusion: There was no substantive impact on vacuum control in water vacuum extraction process by adding Chinese medicines.关键词：vacuum extraction;boiling point;vacuum;the average molar heat of vaporization
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发布时间：2024-09-23 - 摘要：Objective:To study correlation between boiling point and vacuum in vacuum water extraction technology,and compare effects of different medicinal dosage on the vacuum and the average molar heat of vaporization.Method:Theoretical calculation and decompression test determination were used to obtain the vacuum at different boiling point.Then the saturated vapor pressure value and the average molar heat of vaporization in this system were calculated.Result:There are changes between the measured and theoretical values of vacuum with the pure solvent;After added medicinal materials,the measured values of vacuum were lower than the measured values of the pure solvent,the measured values of the average molar heat of vaporization were lower than the values of the pure solvent.Effects of medicinal dosage on the measured value of vacuum had no significant.Conclusion:There was no substantive impact on vacuum control in water vacuum extraction process by adding Chinese medicines.关键词：vacuum extraction;boiling point;vacuum;the average molar heat of vaporization
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发布时间：2024-09-23 - 摘要：Objective: To study on solubilization of dihydromyricetin(DMY) by hydroxpropyl-β-cyclodextrin(HP-β-CD). Method: Inclusion of HP-β-CD on DMY under different temperatures was investigated by phase-solubility method.The content of DMY was determined by HPLC. Result: With increasing of HP-β-CD in aqueous solution,solubility of DMY increased linearly,which showed typical AL-type phase solubility diagram.The molar ratio of DMY and HP-β-CD in the inclusion complex was 1:1.The apparent stability constants of inclusion reaction at 25,35,45℃ were determined as 500.0,944.8,2 449.8 mol·L-1,respectively. Conclusion: The solubility of DMY was improved remarkably by HP-β-CD inclusion.关键词：hydroxpropyl-β-cyclodextrin;dihydromyricetin;phase-solubility method;inclusion complex
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发布时间：2024-09-23 - 摘要：Objective:To study on solubilization of dihydromyricetin（DMY） by hydroxpropyl-β-cyclodextrin（HP-β-CD）.Method:Inclusion of HP-β-CD on DMY under different temperatures was investigated by phase-solubility method.The content of DMY was determined by HPLC.Result:With increasing of HP-β-CD in aqueous solution,solubility of DMY increased linearly,which showed typical AL-type phase solubility diagram.The molar ratio of DMY and HP-β-CD in the inclusion complex was 1∶ 1.The apparent stability constants of inclusion reaction at 25,35,45 ℃ were determined as 500.0,944.8,2 449.8 mol · L-1,respectively.Conclusion:The solubility of DMY was improved remarkably by HP-β-CD inclusion.关键词：hydroxpropyl-β-cyclodextrin;dihydromyricetin;phase-solubility method;inclusion complex
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发布时间：2024-09-23 - 摘要：Objective:To optimize formation technology of compound Lujiao granules.Method:With hygroscopicity,formability,mobility,dissolubility and appearance as comprehensive index,kinds and ratio of excipients were selected by single factor test;With forming rate as index,L9（34） orthogonal test was used to investigate effects of horizontal speed,roller speed and pre-pressure on dry granulation process.Result:Optimum ratio of prescription was extract powder-dextrin-lactose-colloidal silicon dioxide 84.5%∶ 12%∶ 3%∶ 0.5%;The best technology parameters were as follows:vertical speed 10 r·min-1,horizontal speed at 30 r·min-1,roller speed 4 r·min-1,pre-pressure 6 MPa.Conclusion:These prepared granules had good formability,good anti-wet performance,high solubility and color uniformity;This optimized formation technology was simple,feasible and suit to industrial production.关键词：Compound Lujiao granules;dry granulation;orthogonal test;molding technology
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发布时间：2024-09-23 - 摘要：Objective: To optimize formation technology of compound Lujiao granules. Method: With hygroscopicity, formability, mobility, dissolubility and appearance as comprehensive index,kinds and ratio of excipients were selected by single factor test;With forming rate as index,L9(34) orthogonal test was used to investigate effects of horizontal speed,roller speed and pre-pressure on dry granulation process. Result: Optimum ratio of prescription was extract powder-dextrin-lactose-colloidal silicon dioxide 84.5%: 12%: 3%: 0.5%;The best technology parameters were as follows:vertical speed 10 r·min-1,horizontal speed at 30 r·min-1,roller speed 4 r·min-1,pre-pressure 6 MPa. Conclusion: These prepared granules had good formability,good anti-wet performance, high solubility and color uniformity;This optimized formation technology was simple,feasible and suit to industrial production.关键词：Compound Lujiao granules;dry granulation;orthogonal test;molding technology
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of shikimic acid from wastewater,which was produced after volatile oil was extracted from leaves and stems of Star Anise.Method:With the content of shikimic acid as index,orthogonal tests were adopted to optimize technology conditions for removing impurities and pigments by lime paste and powdered activated carbon,then wastewater was enriched by resin 201×7（717） anion exchange resin column,eluted by 1%HCl,collected eluate,concentrated to receive crude products;The crude products was recrystallized by acetic acid,obtained shikimic acid products.Result:Optimum impurity removal process of lime paste was as following:added 5% lime paste as per the westewater volum,incubated for 20 min at 60 ℃ after mixing;Optimum impurity removal process of activated carbon was:added 5% as per the westewater volum incubated for 60 min at 50 ℃ after mixing,operation at any pH.The average yield and purity of shikimic acid were 37.60%,98.84%,respectively.Conclusion:This optimized technology was simple and feasible by deal with wastewater effectively,and it was suitable for industrial production.关键词：leaves of Star Snise;shikimic acid;extraction technology
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction technology of shikimic acid from wastewater,which was produced after volatile oil was extracted from leaves and stems of Star Anise. Method: With the content of shikimic acid as index,orthogonal tests were adopted to optimize technology conditions for removing impurities and pigments by lime paste and powdered activated carbon,then wastewater was enriched by resin 201×7(717) anion exchange resin column,eluted by 1%HCl,collected eluate,concentrated to receive crude products;The crude products was recrystallized by acetic acid,obtained shikimic acid products. Result: Optimum impurity removal process of lime paste was as following:added 5% lime paste as per the westewater volum,incubated for 20 min at 60℃ after mixing;Optimum impurity removal process of activated carbon was:added 5% as per the westewater volum incubated for 60 min at 50℃ after mixing,operation at any pH.The average yield and purity of shikimic acid were 37.60%,98.84%,respectively. Conclusion: This optimized technology was simple and feasible by deal with wastewater effectively,and it was suitable for industrial production.关键词：leaves of Star Snise;shikimic acid;extraction technology
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction technology of compound Zhichan decoction. Method: With the content of rhynchophylline,isorhynchophylline,paeoniflorin as index,which were determined by HPLC,effects of the amount of water,extraction time,extraction times on extraction technology was investigated by L9(34) orthogonal test. Result: Optimal extraction technology conditions were as followings:extracted 2 times with 10 folds the amount of water,1 h per time. Conclusion: This optimized extraction technology was stable and feasible with high extraction rate.关键词：orthogonal test;extraction process;Zhichan decoction;rhynchophylline;isorhynchophylline;paeoniflorin
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of compound Zhichan decoction.Method: With the content of rhynchophylline,isorhynchophylline,paeoniflorin as index,which were determined by HPLC,effects of the amount of water,extraction time,extraction times on extraction technology was investigated by L9（34） orthogonal test.Result: Optimal extraction technology conditions were as followings:extracted 2 times with 10 folds the amount of water,1 h per time.Conclusion: This optimized extraction technology was stable and feasible with high extraction rate.关键词：orthogonal test;extraction process;Zhichan decoction;rhynchophylline;isorhynchophylline;paeoniflorin
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发布时间：2024-09-23 - 摘要：Objective: To study inhibitory activity of raw products and five processed products of Psoralea corylifolia for α-glucosidase. Method: With acarbose as positive control,inhibitory activity was screened by α-glucosidase in vitro inhibitory model. Result: Each parts from raw and processed products of P. corylifolia had some α-glucosidase inhibitory activity,and the activity of them were higher than acarbose.Methanol total extracts of salt steamed P. corylifolia,fried salt P. corylifolia and fried P. corylifolia showed higher inhibitory activity (IC50=23.03,31.26,36.06 μg·L-1,respectively) than methanol total extract of raw P. corylifolia (IC50=46.47 μg·L-1). Petroleum ether extracts of salt steamed P. corylifolia and tripterygium wilfordii method P. corylifolia had higher inhibitory activity (IC50=17.96,34.02 μg·L-1,respectively) than petroleum ether extracts of raw P. corylifolia (IC50=46.94 μg·L-1).Acetic ether extracts of five processed products (salt steamed P. corylifolia,tripterygium wilfordii method P. corylifolia,fried salt P. corylifolia,fried P. corylifolia ang wine fried P. corylifolia) had higher inhibitory activity (IC50=51.27,39.08,40.86,45.54,36.06 μg·L-1,respectively) than acetic ether extracts of raw P. corylifolia (IC50=71.28 μg·L-1).n-Butyl alcohol extacts of salt steamed P. corylifolia and wine fried P. corylifolia showed higher inhibitory activity (IC50=47.52,36.06 μg·L-1,respectively) than n-butyl alcohol extacts of raw P. corylifolia (IC50=51.27 μg·L-1). In addition,the inhibitory activity of each parts extracts was positively correlated with the mass concentration,that was in a concentration dependent manner. Conclusion: Raw products and five processed products of P. corylifolia exhibited certain α-glucosidase inhibitory activity.关键词：Psoralea corylifolia;processed products;α-glucosidase;inhibitory activity
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发布时间：2024-09-23 - 摘要：Objective:To study inhibitory activity of raw products and five processed products of Psoralea corylifolia for α-glucosidase.Method: With acarbose as positive control,inhibitory activity was screened by α-glucosidase in vitro inhibitory model.Result: Each parts from raw and processed products of P.corylifolia had some α-glucosidase inhibitory activity,and the activity of them were higher than acarbose.Methanol total extracts of salt steamed P.corylifolia,fried salt P.corylifolia and fried P.corylifolia showed higher inhibitory activity（IC50=23.03,31.26,36.06 μg·L-1,respectively） than methanol total extract of raw P.corylifolia（IC50=46.47 μg·L-1）.Petroleum ether extracts of salt steamed P.corylifolia and tripterygium wilfordii method P.corylifolia had higher inhibitory activity（IC50=17.96,34.02 μg·L-1,respectively） than petroleum ether extracts of raw P.corylifolia（IC50=46.94 μg·L-1）.Acetic ether extracts of five processed products（salt steamed P.corylifolia,tripterygium wilfordii method P.corylifolia,fried salt P.corylifolia,fried P.corylifolia ang wine fried P.corylifolia） had higher inhibitory activity（IC50=51.27,39.08,40.86,45.54,36.06 μg·L-1,respectively） than acetic ether extracts of raw P.corylifolia（IC50=71.28 μg·L-1）.n-Butyl alcohol extacts of salt steamed P.corylifolia and wine fried P.corylifolia showed higher inhibitory activity（IC50=47.52,36.06 μg·L-1,respectively） than n-butyl alcohol extacts of raw P.corylifolia（IC50=51.27 μg·L-1）.In addition,the inhibitory activity of each parts extracts was positively correlated with the mass concentration,that was in a concentration dependent manner.Conclusion: Raw products and five processed products of P.corylifolia exhibited certain α-glucosidase inhibitory activity.关键词：Psoralea corylifolia;processed products;α-glucosidase;inhibitory activity
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发布时间：2024-09-23 - 摘要：Objective: To verify anti-experimental autoimmune neuritis(EAN) activity of dregs in Yisui Tongjing prescription after being extracted by ethanol and optimize its extraction process. Method: EAN rats model was established,then these rats divided into model group,immunoglobulin group,administration group,these rats were administered by the corresponding method,pathomorphological ultrastructure of rats peripheral nerve was observed by electron microscope.With comprehensive score of dry extract quality and yield of protein as index,orthogonal test was adopted to optimize extraction process of water-soluble constituents in Yisui Tongjing prescription with the amount of water,extraction time and times as factors. Result: The situation of demyelination of the administration group was significantly improved,the structure of myelinated fibers myelin lamellar was neat,at the same time you could still see the change in aggregation of collagen fibers and the newborn myelinated nerve fibers.The best extraction process for water-soluble constituents in Yisui Tongjing prescription was:extracted 3 times with 8 times the amount of water for 3 h each time. Conclusion: Water-soluble constituents in Yisui Tongjing prescription had treatment effect for EAN;This optimized extraction technology was simple,stable and suitable for industrial production.关键词：Yisui Tongjing prescription;experimental autoimmune neuritis;water-soluble constituents;extraction technology
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发布时间：2024-09-23 - 摘要：Objective:To verify anti-experimental autoimmune neuritis（EAN） activity of dregs in Yisui Tongjing prescription after being extracted by ethanol and optimize its extraction process.Method:EAN rats model was established,then these rats divided into model group,immunoglobulin group,administration group,these rats were administered by the corresponding method,pathomorphological ultrastructure of rats peripheral nerve was observed by electron microscope.With comprehensive score of dry extract quality and yield of protein as index,orthogonal test was adopted to optimize extraction process of water-soluble constituents in Yisui Tongjing prescription with the amount of water,extraction time and times as factors.Result:The situation of demyelination of the administration group was significantly improved,the structure of myelinated fibers myelin lamellar was neat,at the same time you could still see the change in aggregation of collagen fibers and the newborn myelinated nerve fibers.The best extraction process for water-soluble constituents in Yisui Tongjing prescription was:extracted 3 times with 8 times the amount of water for 3 h each time.Conclusion:Water-soluble constituents in Yisui Tongjing prescription had treatment effect for EAN;This optimized extraction technology was simple,stable and suitable for industrial production.关键词：Yisui Tongjing prescription;experimental autoimmune neuritis;water-soluble constituents;extraction technology
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction process of compound Weishu capsules(CWC) and establish its quality standard. Method: With yield of dry extract,berberine hydrochloride(BH) and palmatine hydrochloride(PH) as comprehensive evaluation index,orthogonal design was carried out to optimize extraction process by taking the concentration of ethanol,extraction time and liquid-solid ratio as factors,extraction times was investigated by single factor test.Coptidis Rhizoma and Euodiae Fructus in CWC were identified by TLC,the content of BH and PH were determined by HPLC. Result: Optimum extraction process was as following:reflux extracted 3 times with 11 times the amount of 60% ethanol with 1 h each time.TLC identification spots were fairly clear and the blank test showed no interference.The linear range of BH and PH was 3.156-202 and 1.375-88 mg·L-1,respectively with r=0.999 9;The average recovery was 100.2% and 99.7%,respectively with RSD of 1.76% and 1.89%. Conclusion: This optimized technology was stable and feasible with good repeatability,and it could be used in industrial production of CWC. This established quality control method was simple,special,reproducible,and effective for quality control of CWC.关键词：compound Weishu capsules;extraction process;quality standard;berberine hydrochloride;palmatine hydrochloride
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction process of compound Weishu capsules（CWC） and establish its quality standard.Method:With yield of dry extract,berberine hydrochloride（BH） and palmatine hydrochloride（PH） as comprehensive evaluation index,orthogonal design was carried out to optimize extraction process by taking the concentration of ethanol,extraction time and liquid-solid ratio as factors,extraction times was investigated by single factor test.Coptidis Rhizoma and Euodiae Fructus in CWC were identified by TLC,the content of BH and PH were determined by HPLC.Result:Optimum extraction process was as following:reflux extracted 3 times with 11 times the amount of 60% ethanol with 1 h each time.TLC identification spots were fairly clear and the blank test showed no interference.The linear range of BH and PH was 3.156-202 and 1.375-88 mg·L-1,respectively with r=0.999 9;The average recovery was 100.2% and 99.7%,respectively with RSD of 1.76% and 1.89%.Conclusion:This optimized technology was stable and feasible with good repeatability,and it could be used in industrial production of CWC.This established quality control method was simple,special,reproducible,and effective for quality control of CWC.关键词：compound Weishu capsules;extraction process;quality standard;berberine hydrochloride;palmatine hydrochloride
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发布时间：2024-09-23 - 摘要：Objective:To optimize flocculation clarification technology of Sangshen Bunao creams.Method:With the rate of impurity removal,the retention rate of stilbene glucoside and polysaccharide as comprehensive evaluation indexes,taking the degree of concentration,pH value,temperature and chitosan dosage as factors,L9（34） orthogonal test was adopted to optimize flocculation clarification technology of Sangshen Bunao creams by comprehensively weighted grading method.Result:Optimum flocculation clarification process conditions were as followings:water extraction liquid concentrated to 1∶2,flocculation temperature of 50 ℃,per 100 mL concentrated solution plus 1% chitosan solution 10 mL,pH of 4.Conclusion:This flocculation clarification technology was feasible and stable with high rate of impurity removal and high retention rate of active ingredients.关键词：flocculation;chitosan;stilbene glycoside;total polysaccharides;orthogonal test
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发布时间：2024-09-23 - 摘要：Objective: To optimize flocculation clarification technology of Sangshen Bunao creams. Method: With the rate of impurity removal, the retention rate of stilbene glucoside and polysaccharide as comprehensive evaluation indexes,taking the degree of concentration,pH value,temperature and chitosan dosage as factors,L9(34) orthogonal test was adopted to optimize flocculation clarification technology of Sangshen Bunao creams by comprehensively weighted grading method. Result: Optimum flocculation clarification process conditions were as followings:water extraction liquid concentrated to 1:2,flocculation temperature of 50℃,per 100 mL concentrated solution plus 1% chitosan solution 10 mL,pH of 4. Conclusion: This flocculation clarification technology was feasible and stable with high rate of impurity removal and high retention rate of active ingredients.关键词：flocculation;chitosan;stilbene glycoside;total polysaccharides;orthogonal test
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction technology of total alkaloids from Baphicacanthus cusia. Method: Based on single factor test,effects of extraction time,ethanol concentration and liquid-solid ratio on yield of total alkaloids from B. cusia was investigated with a three-factor Box-Behnken center-united design,extraction parameters were optimized by response surface methodology. Result: Optimized extraction technology conditions was as following:extraction time 20.23 min,the concentration of ethanol 83.21 %,liquid-solid ratio 21.16: 1,theoretical yield of total alkaloids could be up to 0.781%.According to actual situation in production,optimum extraction parameters were modified as extraction time of 20.5 min,the concentration of ethanol 83.0%,liquid-solid ratio 21.0: 1,validated experiment showed yield of total alkaloids was (0.78 ± 0.024)% under these conditions,which was in consistency with the theoretic value. Conclusion: This modified extraction parameters were credible and reliable with some practical value.关键词：Baphicacanthus cusia;total alkaloids;response surface methodology;extraction technology
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of total alkaloids from Baphicacanthus cusia.Method:Based on single factor test,effects of extraction time,ethanol concentration and liquid-solid ratio on yield of total alkaloids from B.cusia was investigated with a three-factor Box-Behnken center-united design,extraction parameters were optimized by response surface methodology.Result:Optimized extraction technology conditions was as following:extraction time 20.23 min,the concentration of ethanol 83.21 %,liquid-solid ratio 21.16∶ 1,theoretical yield of total alkaloids could be up to 0.781%.According to actual situation in production,optimum extraction parameters were modified as extraction time of 20.5 min,the concentration of ethanol 83.0%,liquid-solid ratio 21.0∶ 1,validated experiment showed yield of total alkaloids was（0.78 ± 0.024）% under these conditions,which was in consistency with the theoretic value.Conclusion:This modified extraction parameters were credible and reliable with some practical value.关键词：Baphicacanthus cusia;total alkaloids;response surface methodology;extraction technology
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发布时间：2024-09-23 - 摘要：Objective:To prepare Brucea javanica oil self-microemulsion,and investigate its dissolution.Method: Solubility test and gas chromatography were used to select adjuvants,pseudo-ternary phase diagram was used to optmize prescription of the self-microemulsion,and dissolution test of the self-microemulsion was adopted.Result: Optimum prescription of B.javanica oil self-microemulsion was B.javanica oil-caprylic capric triglyceride-polyoxyethylene hydrogenated castor oil-polyethylene glycol 400 12%∶ 12%∶ 65%∶ 11%,particle size of it was<50 nm and it could be completely dissolved in 15-20 minutes.Conclusion: B.javanica oil self-microemulsion had good stability,it improved medication compliance,and this preparation technology was simple and stable.关键词：Brucea javanica oil;self-emulsifying system;pseudo-ternary phase diagram;dissolution
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发布时间：2024-09-23 - 摘要：Objective: To prepare Brucea javanica oil self-microemulsion,and investigate its dissolution. Method: Solubility test and gas chromatography were used to select adjuvants,pseudo-ternary phase diagram was used to optmize prescription of the self-microemulsion,and dissolution test of the self-microemulsion was adopted. Result: Optimum prescription of B. javanica oil self-microemulsion was B. javanica oil-caprylic capric triglyceride-polyoxyethylene hydrogenated castor oil-polyethylene glycol 400 12%: 12%: 65%: 11%,particle size of it was<50 nm and it could be completely dissolved in 15-20 minutes. Conclusion: B. javanica oil self-microemulsion had good stability,it improved medication compliance,and this preparation technology was simple and stable.关键词：Brucea javanica oil;self-emulsifying system;pseudo-ternary phase diagram;dissolution
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发布时间：2024-09-23 - 摘要：Objective: To prepare glycyrrhizin acid phospholipids-bile salts mixed micelles,and optimize its formulation by central composite design-response surface methodology. Method: Glycyrrhizin acid phospholipids-bile salts mixed micelles was prepared by film dispersion method,solubilization of bile salts to phospholipids was investigated by single factor test,solubility of phospholipids in bile salt solution was determined.The molar concentration of bile salts and pH of buffered saline solution were selected as investigation factors,with solubility of glycyrrhizin acid and drug loading of mixed micelles as indexes,formulation was optimized by central composite design test,and fitted by multiple linear and binomial nonlinear equation,then optimum precription ratio was determined by response surface methodology. Result: The best prescription was as following:phospholipids-bile salts 1.19: 1,pH of buffered saline solution 7.4,the molar concentration of bile salts 15 mmol·L-1;Solubility of glycyrrhizin acid 21.12 g·L-1,drug loading of mixed micelles was 53.93%;There was a little deviation between the theoretic value and the measured value,it showed that this model had a good prediction. Conclusion: The solubility of glycyrrhizin acid could significantly increase by preparing bile salts-phospholipids micelles,and this optimized technology was simple and stable.关键词：glycyrrhizin acid;bile salts-phospholipids mixed micelles;central composite design-response surface methodology
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- 1
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发布时间：2024-09-23 - 摘要：Objective:To prepare glycyrrhizin acid phospholipids-bile salts mixed micelles,and optimize its formulation by central composite design-response surface methodology.Method:Glycyrrhizin acid phospholipids-bile salts mixed micelles was prepared by film dispersion method,solubilization of bile salts to phospholipids was investigated by single factor test,solubility of phospholipids in bile salt solution was determined.The molar concentration of bile salts and pH of buffered saline solution were selected as investigation factors,with solubility of glycyrrhizin acid and drug loading of mixed micelles as indexes,formulation was optimized by central composite design test,and fitted by multiple linear and binomial nonlinear equation,then optimum precription ratio was determined by response surface methodology.Result:The best prescription was as following:phospholipids-bile salts 1.19∶ 1,pH of buffered saline solution 7.4,the molar concentration of bile salts 15 mmol · L-1;Solubility of glycyrrhizin acid 21.12 g · L-1,drug loading of mixed micelles was 53.93%;There was a little deviation between the theoretic value and the measured value,it showed that this model had a good prediction.Conclusion:The solubility of glycyrrhizin acid could significantly increase by preparing bile salts-phospholipids micelles,and this optimized technology was simple and stable.关键词：glycyrrhizin acid;bile salts-phospholipids mixed micelles;central composite design-response surface methodology
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- 202
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- 7
发布时间：2024-09-23 - 摘要：Objective: To optimize microwave extraction technology of polysaccharides from seeds of Vicia villosa,and preliminarily investigate its antioxidant activity. Method: With extraction amount of polysaccharides as index,L9(34) orthogonal test was adopted to optimize extraction technology of polysaccharides from seeds of V. villosa with liquid-solid ratio,pH,microwave temperature and time as factors.The content of polysaccharides was determined by phenol sulfuric acid method,antioxidant activity of polysaccharides from seeds of V. villosa was detected by Fention reaction. Result: Optimum extraction conditions for polysaccharides from seeds of V. villosa were as followings:microwave extraction temperature 50℃,microwave treatment time 5 min,solid-liquid ratio 1:25 with pH 7;Under these conditions,extraction amount of polysaccharides was 30.043 mg·g-1.Antioxidant activity test showed that polysaccharides from seeds of V. villosa had strong antioxidant activity. Conclusion: This optimized technology was stable and feasible,it could provide experimental basis for comprehensive utilization and development of seeds of V. villosa.关键词：seeds of Vicia villosa;polysaccharides;microwave;antioxidant activity
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To optimize microwave extraction technology of polysaccharides from seeds of Vicia villosa,and preliminarily investigate its antioxidant activity.Method:With extraction amount of polysaccharides as index,L9（34） orthogonal test was adopted to optimize extraction technology of polysaccharides from seeds of V.villosa with liquid-solid ratio,pH,microwave temperature and time as factors.The content of polysaccharides was determined by phenol sulfuric acid method,antioxidant activity of polysaccharides from seeds of V.villosa was detected by Fention reaction.Result:Optimum extraction conditions for polysaccharides from seeds of V.villosa were as followings:microwave extraction temperature 50 ℃,microwave treatment time 5 min,solid-liquid ratio 1∶25 with pH 7;Under these conditions,extraction amount of polysaccharides was 30.043 mg·g-1.Antioxidant activity test showed that polysaccharides from seeds of V.villosa had strong antioxidant activity.Conclusion:This optimized technology was stable and feasible,it could provide experimental basis for comprehensive utilization and development of seeds of V.villosa.关键词：seeds of Vicia villosa;polysaccharides;microwave;antioxidant activity
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- 153
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- 3
发布时间：2024-09-23 - 摘要：Objective:To optimize molding process of compound Lingdan capsules and establish its standard.Method:Dry granulation technology was adopted,molding technology of compound Lingdan capsules was optimized by single factor test;TLC was used for qualitative identification of Salvia miltiorrhiza and Sophora tonkinensisi;The content of tanshinone IIA and salvianolic acid B was determined by HPLC.Result:The best molding process of compound Lingdan capsules was as following:took prescription amount herbs extracts,added 3% talc,mixed evenly,dry granulating and then filled into 0 capsule.The spots of S.miltiorrhiza and S.tonkinensisi on TLC plates were clear without interference by the blank reference;Anshinone IIA showed a good linear relationship at the range of 0.155-0.003 1 g · L-1,the average recovery was 100.9% with RSD of 1.25%;Salvianolic acid B showed a good linear relationship at the range of 1.750-0.003 5 g·L-1,the average recovery was 101.1% with RSD of 1.25%.Conclusion:This optimized process was simple and stable.This established quality control method was specific and reproducible,which could be applied to quality assessment of compound Lingdan capsules.关键词：compound Lingdan capsules;molding process;quality standard
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- 279
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发布时间：2024-09-23 - 摘要：Objective: To optimize molding process of compound Lingdan capsules and establish its standard. Method: Dry granulation technology was adopted,molding technology of compound Lingdan capsules was optimized by single factor test;TLC was used for qualitative identification of Salvia miltiorrhiza and Sophora tonkinensisi;The content of tanshinone IIA and salvianolic acid B was determined by HPLC. Result: The best molding process of compound Lingdan capsules was as following:took prescription amount herbs extracts,added 3% talc,mixed evenly,dry granulating and then filled into 0 capsule.The spots of S. miltiorrhiza and S. tonkinensisi on TLC plates were clear without interference by the blank reference;Anshinone IIA showed a good linear relationship at the range of 0.155-0.003 1 g·L-1,the average recovery was 100.9% with RSD of 1.25%;Salvianolic acid B showed a good linear relationship at the range of 1.750-0.003 5 g·L-1,the average recovery was 101.1% with RSD of 1.25%. Conclusion: This optimized process was simple and stable.This established quality control method was specific and reproducible,which could be applied to quality assessment of compound Lingdan capsules.关键词：compound Lingdan capsules;molding process;quality standard
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To optimize water extraction technology of Xinmaikang tablets. Method: Heat circulating reflux extraction and concentration unit was taken,with yield of dry extract and the content of synephrine as indexes,the latter was determined by HPLC,effects of extraction time,the amount of water and vapor pressure on extraction technology of Xinmaikang tablets was investigated by orthogonal test. Result: Optimal extraction technology was as following:reflux extracted 4 h with 15 folds the amount of water,vapor pressure 0.15 MPa. Conclusion: This optimized extraction procedure was stable and reliable,and it could be used as industrial production extraction technology of Xinmaikang tablets.关键词：Xinmaikang tablets;heat circulating reflux extraction and concentration unit;extraction technology;orthogonal design;synephrine
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To optimize water extraction technology of Xinmaikang tablets.Method: Heat circulating reflux extraction and concentration unit was taken,with yield of dry extract and the content of synephrine as indexes,the latter was determined by HPLC,effects of extraction time,the amount of water and vapor pressure on extraction technology of Xinmaikang tablets was investigated by orthogonal test.Result:Optimal extraction technology was as following:reflux extracted 4 h with 15 folds the amount of water,vapor pressure 0.15 MPa.Conclusion:This optimized extraction procedure was stable and reliable,and it could be used as industrial production extraction technology of Xinmaikang tablets.关键词：Xinmaikang tablets;heat circulating reflux extraction and concentration unit;extraction technology;orthogonal design;synephrine
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- 66
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- 1
发布时间：2024-09-23 - 摘要：Objective:To analyze the main chemical constituents of volatile oil from barks of Juglans mandshurica.Method:Volatile oil from barks of Juglans mandshurica was extracted by vapour-vapour extraction method（VVE） and steam distillation method（SD）,respectively.These constituents separated from the volatile oil were identified by GC-MS,and the relative percentage of these constituents were calculated by area normalization method.Result:34 components in volatile oil extracted by VVE were separated and identified,which took 97.76% of the total volatile oil;19 constituents in volatile oil extracted by SD were also separated and identified,and their relative percentage was 69.06% of the total volatile oil.Conclusion:The constituents and content of volatile oil extracted by VVE and SD exhibited significant differences,these results provided experimental basis for understanding the constituents and further exploitation of volatile oil from barks of J.mandshurica.关键词：Juglans mandshurica;extraction methods;volatile oil;GC-MS
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- 327
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- 6
发布时间：2024-09-23 - 摘要：Objective: To analyze the main chemical constituents of volatile oil from barks of Juglans mandshurica. Method: Volatile oil from barks of Juglans mandshurica was extracted by vapour-vapour extraction method(VVE) and steam distillation method(SD),respectively.These constituents separated from the volatile oil were identified by GC-MS,and the relative percentage of these constituents were calculated by area normalization method. Result: 34 components in volatile oil extracted by VVE were separated and identified,which took 97.76% of the total volatile oil;19 constituents in volatile oil extracted by SD were also separated and identified,and their relative percentage was 69.06% of the total volatile oil. Conclusion: The constituents and content of volatile oil extracted by VVE and SD exhibited significant differences,these results provided experimental basis for understanding the constituents and further exploitation of volatile oil from barks of J. mandshurica.关键词：Juglans mandshurica;extraction methods;volatile oil;GC-MS
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To optimize ultrasonic extraction process of total alkaloids in Isatis indigotica.Method:With extraction amount of total alkaloids as index,effects of ethanol concentration,liquid-solid ratio,ultrasonic time on extraction technology was investigated by by L9（34） orthogonal test.Result:The influencing order of each factors on ultrasonic-assistant extraction process of total alkaloids was ethanol concentration>extraction time>liquid-solid ratio.Optimum extraction process was as following:extracted 20 min with 10 times the amount of 80% ethanol.Conclusion:This optimized technology was stable and feasible with high extraction rate.关键词：Isatis indigotica;total alkaloids;Orthogonal Design;extraction process
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- 708
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发布时间：2024-09-23 - 摘要：Objective: To optimize ultrasonic extraction process of total alkaloids in Isatis indigotica. Method: With extraction amount of total alkaloids as index,effects of ethanol concentration,liquid-solid ratio,ultrasonic time on extraction technology was investigated by by L9(34) orthogonal test. Result: The influencing order of each factors on ultrasonic-assistant extraction process of total alkaloids was ethanol concentration>extraction time>liquid-solid ratio. Optimum extraction process was as following:extracted 20 min with 10 times the amount of 80% ethanol. Conclusion: This optimized technology was stable and feasible with high extraction rate.关键词：Isatis indigotica;total alkaloids;Orthogonal Design;extraction process
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To isolate and purify the crude polysaccharide from Polygonatum odoratum and then to identify the chemical structure.Method: A polysaccharide-rich extract was obtained by hot water extraction from Polygonatum odoratum and purified by grading ethanol precipitation and collect the precipitate at 80% volume fraction of ethanol,were sequentially purified by DEAE-cellulose chromatography and Sephadex G-100 molecular sieve chromatography and an acidic polysaccharide fraction named POAP80 was obtained.The purity of POAP80 was identified by UV absorption spectrum,IR spectrum and paper chromatogram.The monosaccharide compositions of POAP80 were analyzed by infrared spectroscopy and PMP pre-column derivatization high performance liquid chromatography.Result: An acidic polysaccharide was isolated and purified from Polygonatum odoratum and it was mainly composed of mannose,glucose,galacturonic acid and galactose at a molar ratio of 0.93∶2.65∶29.38∶3.47.Conclusion: POAP80 was found in the Polygonatum odoratum for a novel component.The structure information POAP80 was obtained through the above-mentioned methods,which provides the basis for the further study of the constitution and function of POAP80.关键词：Polygonatum odoratum;polysaccharide;purification;monosaccharide composition
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- 547
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- 20
发布时间：2024-09-23 - 摘要：Objective: To isolate and purify the crude polysaccharide from Polygonatum odoratum and then to identify the chemical structure. Method: A polysaccharide-rich extract was obtained by hot water extraction from Polygonatum odoratum and purified by grading ethanol precipitation and collect the precipitate at 80% volume fraction of ethanol, were sequentially purified by DEAE-cellulose chromatography and Sephadex G-100 molecular sieve chromatography and an acidic polysaccharide fraction named POAP80 was obtained. The purity of POAP80 was identified by UV absorption spectrum, IR spectrum and paper chromatogram. The monosaccharide compositions of POAP80 were analyzed by infrared spectroscopy and PMP pre-column derivatization high performance liquid chromatography. Result: An acidic polysaccharide was isolated and purified from Polygonatum odoratum and it was mainly composed of mannose, glucose, galacturonic acid and galactose at a molar ratio of 0.93: 2.65: 29.38: 3.47. Conclusion: POAP80 was found in the Polygonatum odoratum for a novel component. The structure information POAP80 was obtained through the above-mentioned methods, which provides the basis for the further study of the constitution and function of POAP80.关键词：Polygonatum odoratum;polysaccharide;purification;monosaccharide composition
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To establish quantitative and qualitative determination for the quality control standard of K-CoxB-JN. Method: TLC was used to identify Panacis Quiaquefolii Radix, Ophiopogonis Radix, Raphani Semen and Vaccariae Semen. The content of ginsenoside Rg1, Re, Rb1 in K-CoxB-JN was determined by HPLC-ELSD. An Agilent ZORBAX XDB C18 (4.6 mm×250 mm, 5 μm) was used, and the mobile phase consisted of acetonitrile-0.2% formic acid (gradient elution). The temperature was set at room condition. The parameter of evaporative light-scattering detector: the temperature of drift tube was at 50℃ and the nebulizer nitrogen flow rate was 2.0 L·min-1; gain was 1. Result: The TLC spots were highly clear without the interference of negative sample, and had good separation effect. The linear relationship was obtained within the range of 0.75-3.75, 4.5-30 and 6-40 μg for ginsenoside Rg1, Re, Rb1 respectively (r>0.999). The average recoveries of the three components were 97.10% (RSD 3.96%), 95.15% (RSD 2.21%), 95.60% (RSD 3.00%) respectively. Conclusion: The method appeared to be simple, accurate, sensitive and reliable, and can be used in qualitative and quantitative analysis of K-CoxB-JN.关键词：K-CoxB-JN;TLC;Ginsenoside Rg1;Ginsenoside Re;ginsenoside Rb1;HPLC-ELSD;content determination
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- 1
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发布时间：2024-09-23 - 摘要：Objective:To establish quantitative and qualitative determination for the quality control standard of K-CoxB-JN.Method: TLC was used to identify Panacis Quiaquefolii Radix,Ophiopogonis Radix,Raphani Semen and Vaccariae Semen.The content of ginsenoside Rg1,Re,Rb1 in K-CoxB-JN was determined by HPLC-ELSD.An Agilent ZORBAX XDB C18（4.6 mm×250 mm,5 μm） was used,and the mobile phase consisted of acetonitrile-0.2% formic acid（gradient elution）.The temperature was set at room condition.The parameter of evaporative light-scattering detector: the temperature of drift tube was at 50 ℃ and the nebulizer nitrogen flow rate was 2.0 L · min-1;gain was 1.Result: The TLC spots were highly clear without the interference of negative sample,and had good separation effect.The linear relationship was obtained within the range of 0.75-3.75,4.5-30 and 6-40 μg for ginsenoside Rg1,Re,Rb1 respectively（r>0.999）.The average recoveries of the three components were 97.10%（RSD 3.96%）,95.15%（RSD 2.21%）,95.60%（RSD 3.00%） respectively.Conclusion: The method appeared to be simple,accurate,sensitive and reliable,and can be used in qualitative and quantitative analysis of K-CoxB-JN.关键词：K-CoxB-JN;TLC;Ginsenoside Rg1;Ginsenoside Re;ginsenoside Rb1;HPLC-ELSD;content determination
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- 81
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- 2
发布时间：2024-09-23 - 摘要：Objective:To revise and improve quality standard of Solanum nigrum.Method:According to the research methods of pharmaceutical standard in 2010 version of ’Chinese Pharmacopoeia’ Appendix,S.nigrum was identified by TLC and microscopy.Moisture,ash and acid insoluble ash from 10 batches of S.nigrum were detected,and the content of active ingredients were determined.Result:Microscopic characteristics of S.nigrum was derermined,identification methods of TLC was established;The content of moisture could not more than 10%,acid insoluble ash could not more than 3.0%;Solasonine was linear in the range of 1.002-20.04 μg,but solamargine was linear in 1.023-20.46 μg.Conclusion:The total content of solasonine and solamargine from S.nigrum in different areas had significant difference.This established method could accurately reflect the inherent quality of S.Nigrum,which could be used as the amendments of quality standard for S.nigrum.关键词：Solanum nigrum;quality standard;TLC;HPLC;solasonine;solamargine
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- 417
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- 16
发布时间：2024-09-23 - 摘要：Objective: To revise and improve quality standard of Solanum nigrum. Method: According to the research methods of pharmaceutical standard in 2010 version of 'Chinese Pharmacopoeia’ Appendix, S. nigrum was identified by TLC and microscopy.Moisture,ash and acid insoluble ash from 10 batches of S. nigrum were detected,and the content of active ingredients were determined. Result: Microscopic characteristics of S. nigrum was derermined,identification methods of TLC was established;The content of moisture could not more than 10%,acid insoluble ash could not more than 3.0%;Solasonine was linear in the range of 1.002-20.04 μg,but solamargine was linear in 1.023-20.46 μg. Conclusion: The total content of solasonine and solamargine from S. nigrum in different areas had significant difference.This established method could accurately reflect the inherent quality of S. Nigrum,which could be used as the amendments of quality standard for S. nigrum.关键词：Solanum nigrum;quality standard;TLC;HPLC;solasonine;solamargine
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for the assaying of aconitine in Xiaojin pills. Method: C18 (4.6 mm×250 mm,5 μm) was used with methanol-30 mmol·L-1phosphate solution-triethylamine (45: 55: 0.04) as the mobile phase. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 240 nm. Result: Aconitine concentration presented a good linear range of 0.114-2.28 μg (r=0.999 8). The average recovery was 97.79% and RSD was 2.31%. Conclusion: This method is rapid, simple, accurate and suitable for the quality control of the pill.关键词：Xiaojin pills;aconitine;HPLC
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for the assaying of aconitine in Xiaojin pills.Method: C18（4.6 mm×250 mm,5 μm） was used with methanol-30 mmol · L-1phosphate solution-triethylamine（45∶55∶0.04） as the mobile phase.The flow rate was 1.0 mL · min-1 and the detection wavelength was set at 240 nm.Result: Aconitine concentration presented a good linear range of 0.114-2.28 μg（r=0.999 8）.The average recovery was 97.79% and RSD was 2.31%.Conclusion: This method is rapid,simple,accurate and suitable for the quality control of the pill.关键词：Xiaojin pills;aconitine;HPLC
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- 188
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- 8
发布时间：2024-09-23 - 摘要：Objective: To study the quality standard of Liujing Toutong tablets in order to improve its quality standard. Method: The herbs of preparation were identified by TLC, the content of puerarin in Puerariae Lobatae Radix was determined by HPLC. The separation was performed on shimadzu C18 column with methanol-0.05 mol·L-1 phorsphoric acid solution as mobile phese. The flow rate was 1 mL·min-1. The column was kept at 30℃ and detected at 250 nm. Result: The herbs of preparation could be identified by TLC. Puerarin showed a good linear relationship in a range of 0.047 2-0.378 μg (r=0.999 9). The average recovery was 98.368% (RSD 1.87%). Conclusion: The methods are available with a good reproducibility and can control the quality of this preparation effectively.关键词：Liujing Toutong tablets;quality standard;puerarin;HPLC
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To study the quality standard of Liujing Toutong tablets in order to improve its quality standard.Method: The herbs of preparation were identified by TLC,the content of puerarin in Puerariae Lobatae Radix was determined by HPLC.The separation was performed on shimadzu C18 column with methanol-0.05 mol·L-1 phorsphoric acid solution as mobile phese.The flow rate was 1 mL·min-1.The column was kept at 30 ℃ and detected at 250 nm.Result: The herbs of preparation could be identified by TLC.Puerarin showed a good linear relationship in a range of 0.047 2-0.378 μg（r=0.999 9）.The average recovery was 98.368%（RSD 1.87%）.Conclusion: The methods are available with a good reproducibility and can control the quality of this preparation effectively.关键词：Liujing Toutong tablets;quality standard;puerarin;HPLC
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- 111
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- 5
发布时间：2024-09-23 - 摘要：Objective: To compare the changes of the flavonoids components in Astragali Radix before and after processing. Method: High performance liquid chromatograph was employed for the determination. The column was Kromasil C18 (4.6 mm ×250 mm, 5 μm) and the mobile phase was acetonitrile (A)-water (B) with the gradient elution (0-15 min, 20%-30% A, 15-25 min, 30%-50% A, 25-30 min, 50% A) at a flow rate of 1.0 mL·min-1. The wavelength was set up at 260 nm with the column temperature maintained at 35℃. Result: The calibration curve was linear in the range of 2.120-42.40 mg·L-1 for calycosin-7-O-β-D-glucoside, 1.360-27.20 mg·L-1 for formononetin-7-O-β-D-glucoside, 1.060-21.20 mg·L-1 for calycosin, and 0.360 0-7.200 mg·L-1 for formononetin, respectively, with the average recovery ranged from 95.1% to 98.5%. Honey processing decreases the content of the flavonoids in Astragali Radix and alcohol processing raises the content of calycosin. Rice or salt processing slightly decrease the content of flavonoids in the Astragali Radix with no statistical difference. Conclusion: Honey and alcohol processing change the content of flavonoids in Astragali Radix. Rice and salt processing have no significant influence on the content of flavonoids.关键词：Astragali Radix;processing;flavonoids;HPLC;content determination
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To compare the changes of the flavonoids components in Astragali Radix before and after processing.Method: High performance liquid chromatograph was employed for the determination.The column was Kromasil C18（4.6 mm ×250 mm,5 μm） and the mobile phase was acetonitrile（A）-water（B） with the gradient elution（0-15 min,20%-30% A,15-25 min,30%-50% A,25-30 min,50% A） at a flow rate of 1.0 mL · min-1.The wavelength was set up at 260 nm with the column temperature maintained at 35 ℃.Result: The calibration curve was linear in the range of 2.120-42.40 mg · L-1 for calycosin-7-O-β-D-glucoside,1.360-27.20 mg · L-1 for formononetin-7-O-β-D-glucoside,1.060-21.20 mg · L-1 for calycosin,and 0.360 0-7.200 mg · L-1 for formononetin,respectively,with the average recovery ranged from 95.1% to 98.5%.Honey processing decreases the content of the flavonoids in Astragali Radix and alcohol processing raises the content of calycosin.Rice or salt processing slightly decrease the content of flavonoids in the Astragali Radix with no statistical difference.Conclusion: Honey and alcohol processing change the content of flavonoids in Astragali Radix.Rice and salt processing have no significant influence on the content of flavonoids.关键词：Astragali Radix;processing;flavonoids;HPLC;content determination
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- 653
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- 23
发布时间：2024-09-23 - 摘要：Objective:To establish a method for determining polysaccharides in Atractylodis rhizoma,then compare the content of polysaccharides in Atractylodis rhizoma before and after processing from different sources and to investigate the effects on the content of polysaccharides in Atractylodis rhizoma by stir-frying with wheat bran.Method: The samples were extracted with water;the phenol-sulfuric acid method was used to detect the polysaccharide content.Result: The content of polysaccharides in Atractylodis rhizoma from 17 sources was determined,the glucose concentration value C and absorbance A were linear in the range of 0.004 08-0.012 24 mg · L-1（r=0.999 4）,the average recovery was 101.92%,RSD 2.58%.The content of polysaccharides in Atractylodis rhizoma from various cultivation locations varied from 3.45%-24.6%,and 3.34%-17.45% in its processed product.Conclusion: The content of polysaccharides in Atractylodis rhizoma from different sources was different,the content of polysaccharides in Atractylodis rhizoma decreased after being processed.关键词：Atractylodis rhizome;stir-frying with wheat bran;polysaccharide;content determination
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- 330
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- 13
发布时间：2024-09-23 - 摘要：Objective: To establish a method for determining polysaccharides in Atractylodis rhizoma, then compare the content of polysaccharides in Atractylodis rhizoma before and after processing from different sources and to investigate the effects on the content of polysaccharides in Atractylodis rhizoma by stir-frying with wheat bran. Method: The samples were extracted with water; the phenol-sulfuric acid method was used to detect the polysaccharide content. Result: The content of polysaccharides in Atractylodis rhizoma from 17 sources was determined, the glucose concentration value C and absorbance A were linear in the range of 0.004 08-0.012 24 mg·L-1 (r=0.999 4), the average recovery was 101.92%, RSD 2.58%. The content of polysaccharides in Atractylodis rhizoma from various cultivation locations varied from 3.45%-24.6%, and 3.34%-17.45% in its processed product. Conclusion: The content of polysaccharides in Atractylodis rhizoma from different sources was different, the content of polysaccharides in Atractylodis rhizoma decreased after being processed.关键词：Atractylodis rhizome;stir-frying with wheat bran;polysaccharide;content determination
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To improve the quality for Qingre Jiedu Tablets.Method: Lonicerae Japonicae Flos,Scrophulariae Radix,Forsythiae Fructus,Gardeniae Fructus,Ophiopogonis Radix were indentified by TLC.The content of baicalin and geniposide were determined by HPLC.Cosmosil C18-MS-Ⅱ column was used with the mobile phase of methanol-0.2% phosphatic acid in a gradient manner.The detection wavelength was set up at 238 nm.The flow rate was 1.0 mL · min-1and the column temperature was set at 30 ℃.Result: The average recovery was 95.4% with RSD 2.2% for baicalin,99.3% with RSD 1.7% for geniposide（n=6）.Conclusion: The method is simple,exclusive and accurate,which can be used for determination and quality control for Qingrejiedu Tablets.关键词：Qingre Jiedu Tablets;quality standard;TLC;HPLC
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- 147
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- 5
发布时间：2024-09-23 - 摘要：Objective: To improve the quality for Qingre Jiedu Tablets. Method: Lonicerae Japonicae Flos, Scrophulariae Radix, Forsythiae Fructus, Gardeniae Fructus, Ophiopogonis Radix were indentified by TLC. The content of baicalin and geniposide were determined by HPLC. Cosmosil C18-MS-Ⅱ column was used with the mobile phase of methanol-0.2% phosphatic acid in a gradient manner. The detection wavelength was set up at 238 nm. The flow rate was 1.0 mL·min-1and the column temperature was set at 30℃. Result: The average recovery was 95.4% with RSD 2.2% for baicalin, 99.3% with RSD 1.7% for geniposide (n=6). Conclusion: The method is simple, exclusive and accurate, which can be used for determination and quality control for Qingrejiedu Tablets.关键词：Qingre Jiedu Tablets;quality standard;TLC;HPLC
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- 1
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发布时间：2024-09-23 - 摘要：Objective:To establish a method for determination of total sugar and reducing sugar in processed Fructus Trichosanthis,and to detect and compare the content of sugars with the methods.Method: The content of total sugar and reducing sugar was determined by phenol-sulfuric acid method and 3,5-dinitrosalicylic acid（DNS） method,respectively.Result: The content of total sugar and reducing sugar was 16.17%-32.65% and 3.29%-13.29%,respectively,in 18 batches of processed Fructus Trichosanthis purchased from 15 provinces.Conclusion: These methods are easily operated and reliable.The content of total sugar and reducing sugar in processed Fructus Trichosanthis with good quality is above 25% and 9%,respectively.The content of total sugar and reducing sugar can explain the traditional opinion that Fructus Trichosanthis with higher sugar content is of better quality.关键词：Fructus Trichosanthis;total sugar;reducing sugar;phenol-sulfuric acid method;3,5-dinitrosalicylic acid method;Trichosanthes kirilowii
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- 1828
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发布时间：2024-09-23 - 摘要：Objective: To establish a method for determination of total sugar and reducing sugar in processed Fructus Trichosanthis, and to detect and compare the content of sugars with the methods. Method: The content of total sugar and reducing sugar was determined by phenol-sulfuric acid method and 3,5-dinitrosalicylic acid (DNS) method, respectively. Result: The content of total sugar and reducing sugar was 16.17%-32.65% and 3.29%-13.29%, respectively, in 18 batches of processed Fructus Trichosanthis purchased from 15 provinces. Conclusion: These methods are easily operated and reliable. The content of total sugar and reducing sugar in processed Fructus Trichosanthis with good quality is above 25% and 9%, respectively. The content of total sugar and reducing sugar can explain the traditional opinion that Fructus Trichosanthis with higher sugar content is of better quality.关键词：Fructus Trichosanthis;total sugar;reducing sugar;phenol-sulfuric acid method;3,5-dinitrosalicylic acid method;Trichosanthes kirilowii
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To evaluate the quality of cultivated Gentiana in Ningxia Province by the comparative study of effective components in different varieties and wild Gentiana.Method: The content of gentiopicroside and loganin acid was determined according to the method listed in 2010 version of Chinese Pharmacopoeia.The amino acid content was determined by automatic amino acid analyzer,and the content of polysaccharide was determined by anthrone-sulfuric acid method.Result: The content of gentiopicroside and loganin acid in three varieties of Gentiana cultivated in Ningxia was higher than prescribed limits in Chinese Pharmacopoeia,and there were no significant differences in amino acid content,and there were significant differences in the content of polysaccharides compared with wild Gentiana.Conclusion: The quality of three kinds of Gentiana macrophylla cultivated in Ningxia is good.The medicinal value of amino acids and polysaccharides in Gentiana macrophylla can be further researched and utilized.关键词：planting Gentiana;wild Gentiana macrophylla;effective component;comparative study
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- 257
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- 13
发布时间：2024-09-23 - 摘要：Objective: To evaluate the quality of cultivated Gentiana in Ningxia Province by the comparative study of effective components in different varieties and wild Gentiana. Method: The content of gentiopicroside and loganin acid was determined according to the method listed in 2010 version of Chinese Pharmacopoeia. The amino acid content was determined by automatic amino acid analyzer, and the content of polysaccharide was determined by anthrone-sulfuric acid method. Result: The content of gentiopicroside and loganin acid in three varieties of Gentiana cultivated in Ningxia was higher than prescribed limits in Chinese Pharmacopoeia, and there were no significant differences in amino acid content, and there were significant differences in the content of polysaccharides compared with wild Gentiana. Conclusion: The quality of three kinds of Gentiana macrophylla cultivated in Ningxia is good. The medicinal value of amino acids and polysaccharides in Gentiana macrophylla can be further researched and utilized.关键词：planting Gentiana;wild Gentiana macrophylla;effective component;comparative study
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To study the HPLC fingerprint of leaf of Picria fel-tarrae in different growth periods. Method: High performance of liquid chromatography (HPLC) was adopted, the separation was performed on C18 (4.6 mm×250 mm,5 μm) column with acetonitrile-0.1% aqueous acetic acid solution as mobile phase using a gradient elution; the detection wavelength was set at 264 nm, the column temperature was set at 25℃ and the flow rate was 1 mL·min-1; the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM (Version 2004A). Result: In the fingerprint,17 common peaks were confirmed and the similarity of 10 batches of leaf of Picria fel-tarrae. were above 0.9. Conclusion: The experiment result could provide experimental data for the quality control method of the leaf of Picria fel-tarrae. and for optimum collection time and actualizing standard planting of Picria fel-tarrae.关键词：leaf of Picria fel-tarrae Lour.;fingerprint;HPLC
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- 1
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发布时间：2024-09-23 - 摘要：Objective:To study the HPLC fingerprint of leaf of Picria fel-tarrae in different growth periods.Method:High performance of liquid chromatography（HPLC） was adopted,the separation was performed on C18（4.6 mm×250 mm,5 μm） column with acetonitrile-0.1% aqueous acetic acid solution as mobile phase using a gradient elution;the detection wavelength was set at 264 nm,the column temperature was set at 25 ℃ and the flow rate was 1 mL · min-1;the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM（Version 2004A）.Result: In the fingerprint,17 common peaks were confirmed and the similarity of 10 batches of leaf of Picria fel-tarrae.were above 0.9.Conclusion: The experiment result could provide experimental data for the quality control method of the leaf of Picria fel-tarrae.and for optimum collection time and actualizing standard planting of Picria fel-tarrae.关键词：leaf of Picria fel-tarrae Lour.;fingerprint;HPLC
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- 177
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- 4
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for simultaneous determination of rutin,forsythoside A and phillyrin of Forsythiae Fructus and its extract.Method: The procedure of HPLC-PDA was performed on the chromatographic column of Sunfire C18（4.6 mm×150 mm,5 μm）,and the mobile phase in the gradient elution（0 min,14%A;15 min,18%A;40 min,30%A） was acetonitrile（A）-0.2% acetic acid solution（B）.The flow rate was 1.0 mL · min-1;the temperature was kept at 40 ℃,and the detection wavelength was set at 354,329 nm and 277 nm respectively.Result: Rutin,forsythoside A and phillyrin were linear in the range of 0.1-2.0,0.12-2.4 μg and 0.05-1.0 μg respectively.The average recoveries were 98.63%,98.97% and 102.18% respectively（RSD 7.94%,0.93% and 1.63%）.Conclusion: The method is accurate with higher repeatability,which can be used in the quality control of Forsythiae Fructus and its extract.关键词：HPLC-DAD;rutin;forsythoside A;phillyrin
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- 446
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- 16
发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for simultaneous determination of rutin, forsythoside A and phillyrin of Forsythiae Fructus and its extract. Method: The procedure of HPLC-PDA was performed on the chromatographic column of Sunfire C18 (4.6 mm×150 mm, 5 μm), and the mobile phase in the gradient elution (0 min, 14%A; 15 min, 18%A; 40 min, 30%A) was acetonitrile(A)-0.2% acetic acid solution (B). The flow rate was 1.0 mL·min-1; the temperature was kept at 40℃, and the detection wavelength was set at 354, 329 nm and 277 nm respectively. Result: Rutin, forsythoside A and phillyrin were linear in the range of 0.1-2.0, 0.12-2.4 μg and 0.05-1.0 μg respectively. The average recoveries were 98.63%, 98.97% and 102.18% respectively (RSD 7.94%, 0.93% and 1.63%). Conclusion: The method is accurate with higher repeatability, which can be used in the quality control of Forsythiae Fructus and its extract.关键词：HPLC-DAD;rutin;forsythoside A;phillyrin
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To establish the method for determination of luteolin in Caulis et Folium Stauntoniae. Method: The chromatographic conditions was as follows: a Dikma Diamonsil C18 (4.6 mm×250 mm,5 μm) column,a mobile phase of acetonitrile-0.1% H3PO4 (26: 74); and a UV detector at 350 nm. Result: Luteolin was linear in the ranger of 0.067 75-2.168 μg (r=0.999 9); the average recovery of 99.25% (RSD 1.65%), the precision with RSD 2.22%, repetition with RSD 1.24% and stability with RSD 2.58% were obtained. Conclusion: The method is accurate,reliable,and can be used for quality control of the production.关键词：Caulis et Folium Stauntoniae;luteolin;HPLC
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish the method for determination of luteolin in Caulis et Folium Stauntoniae.Method: The chromatographic conditions was as follows: a Dikma Diamonsil C18（4.6 mm×250 mm,5 μm） column,a mobile phase of acetonitrile-0.1% H3PO4（26∶74）;and a UV detector at 350 nm.Result: Luteolin was linear in the ranger of 0.067 75-2.168 μg（r=0.999 9）;the average recovery of 99.25%（RSD 1.65%）,the precision with RSD 2.22%,repetition with RSD 1.24% and stability with RSD 2.58% were obtained.Conclusion: The method is accurate,reliable,and can be used for quality control of the production.关键词：Caulis et Folium Stauntoniae;luteolin;HPLC
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- 145
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- 1
发布时间：2024-09-23 - 摘要：Objective:To establish the HPLC fingerprint of Qingfei Yihuo Tablets.Method: Ultrasphere TM-ODS-C18 column（4.6 mm ×250 mm,5 μm） was used,the mobile phase was 0.1% phosphoric acid solution（A）-acetonitrile（B） gradient elution,the detection wavelength was set at 270 nm.The flow rate was 1 mL ·min-1,and column temperature was kept at 40 ℃.Result: Fingprints of the tablets from different manufacturers varies significantly,which keep stable at a certain level from the same manufacture.Conclusion: The method can be used for the determination of different source of Qingfei Yihuo Tablets and can provide a feasible method for comprehensive quality evaluation and production process monitoring.关键词：Qingfei Yihuo Tablets;fingerprint;HPLC;similarity
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- 140
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- 2
发布时间：2024-09-23 - 摘要：Objective: To establish the HPLC fingerprint of Qingfei Yihuo Tablets. Method: Ultrasphere TM-ODS-C18 column (4.6 mm ×250 mm, 5 μm) was used, the mobile phase was 0.1% phosphoric acid solution (A)-acetonitrile (B) gradient elution, the detection wavelength was set at 270 nm. The flow rate was 1 mL·min-1, and column temperature was kept at 40℃. Result: Fingprints of the tablets from different manufacturers varies significantly, which keep stable at a certain level from the same manufacture. Conclusion: The method can be used for the determination of different source of Qingfei Yihuo Tablets and can provide a feasible method for comprehensive quality evaluation and production process monitoring.关键词：Qingfei Yihuo Tablets;fingerprint;HPLC;similarity
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To study the antimicrobial activity of different extracts of Trapa acornis against SA, MRSA and ESBLs-SA in vitro. Method: The MIC (Minimun Inhibitory Concentration) of different extracts of T. acornis against SA, MRSA and ESBLs-SA were detected by K-B method, and The IC50 (half maximal Inhibitory Concentration of a Substance) of different extracts of T. acornis against SA, MRSA and ESBLs-SA were detected by solution culture method. Result: The petroleum ether (TASPE), EtOAC (TASEA) and n-butyl alcohol (TASBU) extracts exhibited significant inhibitory effects. TASPE showed the best antimicrobial activity of SA, MRSA and EsBLs-SA with IC50 value of 0.81, 0.62, 0.34 g·L-1 respeetively. Conclusion: Different extracts of T. acornis have the antimicrobial activity.关键词：Trapa acornis;antimicrobial activity;SA;MRSA;ESBLs
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To study the antimicrobial activity of different extracts of Trapa acornis against SA,MRSA and ESBLs-SA in vitro.Method:The MIC（Minimun Inhibitory Concentration） of different extracts of T.acornis against SA,MRSA and ESBLs-SA were detected by K-B method,and The IC50（half maximal Inhibitory Concentration of a Substance） of different extracts of T.acornis against SA,MRSA and ESBLs-SA were detected by solution culture method.Result:The petroleum ether（TASPE）,EtOAC（TASEA） and n-butyl alcohol（TASBU） extracts exhibited significant inhibitory effects.TASPE showed the best antimicrobial activity of SA,MRSA and EsBLs-SA with IC50 value of 0.81,0.62,0.34 g · L-1 respeetively.Conclusion:Different extracts of T.acornis have the antimicrobial activity.关键词：Trapa acornis;antimicrobial activity;SA;MRSA;ESBLs
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- 122
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- 10
发布时间：2024-09-23 - 摘要：Objective: To establish a determination method of paeonol and paeoniflorin content in GFW and to compare its content discrepancy in GFW from different pharmaceutical factories. Method: HPLC was used to simultaneously determine the content of paeonol and paeoniflorin. An Elite Hypersil BDS C18(4.6 mm×250 mm, 5 μm) column was used for content determination. The mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution. Flow rate was 1.0 mL·min-1. The detection wavelength was set at 230 nm (0-17 min)→274 nm (17-26 min). The injection volume was 20 μL and column temperature was maintained at 30℃. Result: The linear range of paeonol was 2.02-22.40 μg (r=0.999 9); the linear range of paeoniflorin was 2.40-20.26 μg (r=0.999 9). The average recovery was 99.5%, 99.4%, and RSD was 0.96%, 0.81% (n=9) respectively. Conclusion: The established chromatographic method which had good repeatability can be used to determine the content of paeonol and paeoniflorin in GFW simply and accurately and can be used to evaluate the quality of GFW from different pharmaceutical factories.关键词：Guizhi Fuling pills;HPLC;paeonol;paeoniflorin;content determination
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a determination method of paeonol and paeoniflorin content in GFW and to compare its content discrepancy in GFW from different pharmaceutical factories.Method: HPLC was used to simultaneously determine the content of paeonol and paeoniflorin.An Elite Hypersil BDS C18（4.6 mm×250 mm,5 μm） column was used for content determination.The mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution.Flow rate was 1.0 mL·min-1.The detection wavelength was set at 230 nm（0-17 min）→274 nm（17-26 min）.The injection volume was 20 μL and column temperature was maintained at 30 ℃.Result: The linear range of paeonol was 2.02-22.40 μg（r=0.999 9）;the linear range of paeoniflorin was 2.40-20.26 μg（r=0.999 9）.The average recovery was 99.5%,99.4%,and RSD was 0.96%,0.81%（n=9） respectively.Conclusion: The established chromatographic method which had good repeatability can be used to determine the content of paeonol and paeoniflorin in GFW simply and accurately and can be used to evaluate the quality of GFW from different pharmaceutical factories.关键词：Guizhi Fuling pills;HPLC;paeonol;paeoniflorin;content determination
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- 293
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- 15
发布时间：2024-09-23 - 摘要：Objective:To establish and perfect a method for determining the content of total atractylosides in Xanthium sibiricum.Method:Total atractylosides was extracted with water,the content of total atractylosides was determined by UV with carbxyatractyloside as a reference substance.Result:Absorbance and the concentration of carboxyl atractyloside showed good linear relationship in the range of 0-0.24 g · L-1（r=0.998 2）,the average recovery was 100.99% with RSD 3.57%.Conclusion: This method was simple and affordable with good repeatability.关键词：Xanthium sibiricum;total atractylosides;UV;determination
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- 349
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- 8
发布时间：2024-09-23 - 摘要：Objective: To establish and perfect a method for determining the content of total atractylosides in Xanthium sibiricum. Method: Total atractylosides was extracted with water,the content of total atractylosides was determined by UV with carbxyatractyloside as a reference substance. Result: Absorbance and the concentration of carboxyl atractyloside showed good linear relationship in the range of 0-0.24 g·L-1 (r=0.998 2),the average recovery was 100.99% with RSD 3.57%. Conclusion: This method was simple and affordable with good repeatability.关键词：Xanthium sibiricum;total atractylosides;UV;determination
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for determination of gallic acid and quercetin in Houerhuan Xiaoyan Jiaonang.Method: The analytical column was Hypersil BDS-C18（4.6 mm×150 mm,5 μm）,and the mobile phase was methanol-phosphate solution with the gradient elution.The flow rate was 1.0 mL · min-1 and the detection wavelength was 254 nm.The column temperature was kept at 30 ℃,and the sample volumn was 10 μL.Result: A good linearity was obtained in the range of 15.0-180.0 mg · L-1（r=0.999 6） and 1.25-15.0 mg · L-1（r=0.999 7） for gallic acid and quercetin respectively,and the average recovery was 98.2%（RSD 0.7%） and 99.2%（RSD 0.5%） respectively.Conclusion: The method is convenient,accurate,precise and can be used in the content determination of gallic acid and quercetin in Pithecellobium clypearia antiphlogistic capsule at the same time,and can be used for the quality control of this capsule.关键词：HPLC;Pithecellobium clypearia antiphlogistic capsule;gallic acid;quercetin
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- 156
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- 7
发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for determination of gallic acid and quercetin in Houerhuan Xiaoyan Jiaonang. Method: The analytical column was Hypersil BDS-C18(4.6 mm×150 mm, 5 μm), and the mobile phase was methanol-phosphate solution with the gradient elution. The flow rate was 1.0 mL·min-1 and the detection wavelength was 254 nm. The column temperature was kept at 30℃, and the sample volumn was 10 μL. Result: A good linearity was obtained in the range of 15.0-180.0 mg·L-1 (r=0.999 6) and 1.25-15.0 mg·L-1 (r=0.999 7) for gallic acid and quercetin respectively, and the average recovery was 98.2% (RSD 0.7%) and 99.2% (RSD 0.5%) respectively. Conclusion: The method is convenient, accurate, precise and can be used in the content determination of gallic acid and quercetin in Pithecellobium clypearia antiphlogistic capsule at the same time, and can be used for the quality control of this capsule.关键词：HPLC;Pithecellobium clypearia antiphlogistic capsule;gallic acid;quercetin
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for determination of ephedrine hydrochloride in Fengliaoxing Fengshi Dieda wine. Method: The chromatographic column was Yilite C18(4.6 mm×200 mm, 5 μm); the mobile phase was acetonitril-0.1% phosphoric acid (0.1% triethameamine) (3: 97); the flow rate was 1 mL·min-1; the detecting wavelength was set at 205 nm, and the column temperature was kept at 30℃. Result: The linear range of ephedrine hydrochloride was 0.188-0.940 μg. The average recovery was 99.39% and RSD was 1.38% (n=6). Conclusion: The method established was accuracy, sensitive and simple. It can be used for quality control of Fengliaoxing Fengshi Dieda wine.关键词：Fengliaoxing Fengshi Dieda wine;ephedrine hydrochloride;HPLC
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for determination of ephedrine hydrochloride in Fengliaoxing Fengshi Dieda wine.Method: The chromatographic column was Yilite C18（4.6 mm×200 mm,5 μm）;the mobile phase was acetonitril-0.1% phosphoric acid（0.1% triethameamine）（3∶ 97）;the flow rate was 1 mL · min-1;the detecting wavelength was set at 205 nm,and the column temperature was kept at 30 ℃.Result: The linear range of ephedrine hydrochloride was 0.188-0.940 μg.The average recovery was 99.39% and RSD was 1.38%（n=6）.Conclusion: The method established was accuracy,sensitive and simple.It can be used for quality control of Fengliaoxing Fengshi Dieda wine.关键词：Fengliaoxing Fengshi Dieda wine;ephedrine hydrochloride;HPLC
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- 193
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- 7
发布时间：2024-09-23 - 摘要：Objective: The self contrast and correction factor method was established to determine the content of the related substances in Compound Omeprazole Chewable Tablet. Method: Chromatography was carried out by reversed-phase technique on an Agilent XDB C8 column (4.6 mm×150 mm, 5 μm) with a mobile phase composed of 0.01 mol·L-1 disodium hydrogen phosphate (adjusted to pH 7.6 with phosphate acid) and acetonitrile in the ratio of (75: 25) pumped at a flow-rate of 1.0 mL·min-1. The detection was carried out at 280 nm and a column temperature of 35℃. The injection volume was 20 μL. The slope of linear equation was used to determine the correction factor between impurity A, B, C, D, E and omeprazole. The relative retention time was used to determine the position of impurities. Result: The relative retention time between impurity A, B, C, D, E and Omeprazole was 0.3, 0.9, 3.4, 0.8 and 0.4, respectively. The correction factor of impurity A, B, C, D, E was 1.45, 0.66, 1.21, 1.39 and 0.80, respectively. The content of impurity D was 0.023% in three batches of samples. Other impurities weren't detected. Conclusion: The method was simple, efficient and accurate for analyzing the related substances in Compound Omeprazole Chewable Tablet.关键词：omeprazole;the related substances;the correction factor;the self contrast
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- 1
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发布时间：2024-09-23 - 摘要：Objective:The self contrast and correction factor method was established to determine the content of the related substances in Compound Omeprazole Chewable Tablet.Method: Chromatography was carried out by reversed-phase technique on an Agilent XDB C8 column（4.6 mm×150 mm,5 μm） with a mobile phase composed of 0.01 mol · L-1 disodium hydrogen phosphate（adjusted to pH 7.6 with phosphate acid） and acetonitrile in the ratio of（75∶25） pumped at a flow-rate of 1.0 mL · min-1.The detection was carried out at 280 nm and a column temperature of 35 ℃.The injection volume was 20 μL.The slope of linear equation was used to determine the correction factor between impurity A,B,C,D,E and omeprazole.The relative retention time was used to determine the position of impurities.Result: The relative retention time between impurity A,B,C,D,E and Omeprazole was 0.3,0.9,3.4,0.8 and 0.4,respectively.The correction factor of impurity A,B,C,D,E was 1.45,0.66,1.21,1.39 and 0.80,respectively.The content of impurity D was 0.023% in three batches of samples.Other impurities weren’t detected.Conclusion: The method was simple,efficient and accurate for analyzing the related substances in Compound Omeprazole Chewable Tablet.关键词：omeprazole;the related substances;the correction factor;the self contrast
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- 404
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- 7
发布时间：2024-09-23 - 摘要：To provide the chemical data for resources development of Stephania mashanica,its chemical constituents were investigated; Method: The powered tuber of S. mashanica were extracted with 2% H2SO4-H2O and purified by silica gel, alumina oxide and Sephadex LH-20 column chromatograph. The structures were elucidated on the basis of spectral analysis and physico-chemical evidence. Result: Seven alkaloids were isolated from the thin sulfuric acid extracts of the fresh roots of Stephania mashanica, these alkaloids were identified as dicentrine (1), (() -crebanine (2), sinoacutine (3), tetrahydrocolumbamine (4), tetrahydropalmatine (5), palmatine (6), and jatrorrhizine (7). Conclusion: compounds 2-7 were obtained from this plant for the first time.关键词：Stephania mashanica;chemical constituents;alkaloids
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- 1
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- 0
发布时间：2024-09-23 - 摘要：To provide the chemical data for resources development of Stephania mashanica,its chemical constituents were investigated;Method: The powered tuber of S.mashanica were extracted with 2% H2SO4-H2O and purified by silica gel,alumina oxide and Sephadex LH-20 column chromatograph.The structures were elucidated on the basis of spectral analysis and physico-chemical evidence.Result: Seven alkaloids were isolated from the thin sulfuric acid extracts of the fresh roots of Stephania mashanica,these alkaloids were identified as dicentrine（1）,(（）-crebanine（2）,sinoacutine（3）,tetrahydrocolumbamine（4）,tetrahydropalmatine（5）,palmatine（6）,and jatrorrhizine（7）.Conclusion: compounds 2-7 were obtained from this plant for the first time.关键词：Stephania mashanica;chemical constituents;alkaloids
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- 235
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- 7
发布时间：2024-09-23 - 摘要：Objective:Inquiry into the correlation between residues（SO2） and the effective contents of imperatorin and oxypecedanin in Angelica Dahurica Radix after sulfur fumigated to provide scientific bases for formulating the scientific,rational,objective,controllable standards of sulfur dioxide limits.Method:To dynamic determine the residue of sulfur dioxide by appendix in 2010 version of ’Chinese Pharmacopoeia’ and the content of imperatorin & oxypeucedanin by HPLC.To correlation analyze the residue of sulfur dioxide and effective content by curve regression.Result: Residues（SO2） in samples without sulfur-fumigated is lower than sulfur-fumigated samples and imperatorin & oxypeucedanin in samples without sulfur-fumigated is higher than sulfur-fumigated samples.As the amount of sulfur and fumigating time increase,residues（SO2） increase and imperatorin & oxypeucedanin decrease.Conclusion: The experiment study the regulation of residues（SO2） and chemical contents with sulfur fumigation deepening to laid the foundation for the in-depth study.关键词：Angelica Dahurica Radix;sulfur fumigation;sulfur dioxide residues;Imperatorin;oxypeucedanin
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- 712
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- 43
发布时间：2024-09-23 - 摘要：Objective: Inquiry into the correlation between residues (SO2) and the effective contents of imperatorin and oxypecedanin in Angelica Dahurica Radix after sulfur fumigated to provide scientific bases for formulating the scientific, rational, objective, controllable standards of sulfur dioxide limits. Method: To dynamic determine the residue of sulfur dioxide by appendix in 2010 version of 'Chinese Pharmacopoeia’ and the content of imperatorin & oxypeucedanin by HPLC. To correlation analyze the residue of sulfur dioxide and effective content by curve regression. Result: Residues (SO2) in samples without sulfur-fumigated is lower than sulfur-fumigated samples and imperatorin & oxypeucedanin in samples without sulfur-fumigated is higher than sulfur-fumigated samples. As the amount of sulfur and fumigating time increase, residues (SO2) increase and imperatorin & oxypeucedanin decrease. Conclusion: The experiment study the regulation of residues (SO2) and chemical contents with sulfur fumigation deepening to laid the foundation for the in-depth study.关键词：Angelica Dahurica Radix;sulfur fumigation;sulfur dioxide residues;Imperatorin;oxypeucedanin
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- 1
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发布时间：2024-09-23 - 摘要：Objective: To study correlation between the GC-MS chromatograms of polysaccharide and Cold-Heat nature of traditional Chinese medicine(TCM). Method: The polysaccharide hydrolysis composition acetylation GC-MS fingerprints of 30 kinds of hot herbs and 30 kinds of cold herbs were determined. Cold-hot medicine property (CHMP) markers were determined by the best discriminant model. Result: The best statistical identification model for GC-MS of polysaccharide hydrolysis composition acetylation was PLS-DA. The corresponding cold nature retention time was 3.489, 3.529, 3.557, 3.695, 3.849, 3.986, 6.047, 6.132, 6.241, 6.510, 6.836, 7.294,12.564 min. The corresponding hot nature retention time was 3.523, 3.861, 3.929, 5.526, 6.006, 6.269, 6.516, 6.562, 6.842, 7.397, 10.778,10.784 min. Conclusion: There is relationship between the GC-MS chromatograms of polysaccharide hydrolysis composition acetylation and Cold-heat nature of TCM.关键词：Cold-Hot nature;carbohydrate components;GC-MS;discriminant analysis;model
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发布时间：2024-09-23 - 摘要：Objective:To study correlation between the GC-MS chromatograms of polysaccharide and Cold-Heat nature of traditional Chinese medicine（TCM）.Method: The polysaccharide hydrolysis composition acetylation GC-MS fingerprints of 30 kinds of hot herbs and 30 kinds of cold herbs were determined.Cold-hot medicine property（CHMP） markers were determined by the best discriminant model.Result: The best statistical identification model for GC-MS of polysaccharide hydrolysis composition acetylation was PLS-DA.The corresponding cold nature retention time was 3.489,3.529,3.557,3.695,3.849,3.986,6.047,6.132,6.241,6.510,6.836,7.294,12.564 min.The corresponding hot nature retention time was 3.523,3.861,3.929,5.526,6.006,6.269,6.516,6.562,6.842,7.397,10.778,10.784 min.Conclusion: There is relationship between the GC-MS chromatograms of polysaccharide hydrolysis composition acetylation and Cold-heat nature of TCM.关键词：Cold-Hot nature;carbohydrate components;GC-MS;discriminant analysis;model
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- 458
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- 15
发布时间：2024-09-23 - 摘要：Objective: To analyze and identify the alkaloids in Strychnos nux-vomica. Method: HPLC-ESI-MS-MS was adopted to induce the characteristic fragmentation pathways of four kinds of alkaloid reference standards, which was applied to identify the alkaloids in S. nux-vomica. Result: The diversities of their structures lead to different ESI-MS-MS fragmentation. According to the fragmentation pathways, retention times, mass charge ratio and MS-MS data, eighteen alkaloids including 8 strychnine type, 2 pseudo type, 5 N→O type and 3 N-Me type were identified from the extract of S. nux-vomica. Conclusion: This study is helpful for the quality analysis and control of the extract of S. nux-vomica.关键词：Strychnos nux-vomica;alkaloids;LC-MS;ESI-MS-MS;fragmentation pathways;structural identification
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发布时间：2024-09-23 - 摘要：Objective:To analyze and identify the alkaloids in Strychnos nux-vomica.Method: HPLC-ESI-MS-MS was adopted to induce the characteristic fragmentation pathways of four kinds of alkaloid reference standards,which was applied to identify the alkaloids in S.nux-vomica.Result: The diversities of their structures lead to different ESI-MS-MS fragmentation.According to the fragmentation pathways,retention times,mass charge ratio and MS-MS data,eighteen alkaloids including 8 strychnine type,2 pseudo type,5 N→O type and 3 N-Me type were identified from the extract of S.nux-vomica.Conclusion: This study is helpful for the quality analysis and control of the extract of S.nux-vomica.关键词：Strychnos nux-vomica;alkaloids;LC-MS;ESI-MS-MS;fragmentation pathways;structural identification
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- 647
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- 13
发布时间：2024-09-23 - 摘要：Objective:To study the relationship between the chemical components and the planting area of Corni Fructus,Method: Samples collected from 14 localities of three main production areas of Henan,Zhejiang and Shanxi province were obtained.The contents of main chemical components including loganin,ursolic aicd,polysaccharide,tannins,organic acid,water-soluble extraction and alcohol-soluble extraction were determined to analyze the correlation between chemical components and meteorological factors by PASW STATISTICS 18 software.Result: The results showed the content of ursolic acid was negatively correlated with sunshine hours,polysaccharide content was positively with sunshine hours and average annual temperature,water-soluble extraction was positively correlated with average annual temperature.Conclusion: The results provided theoretical support for good quality cultivation of Corni Fructus.关键词：Corni Fructus;chemical components;meteorological factors;correlation
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- 279
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- 11
发布时间：2024-09-23 - 摘要：Objective: To study the relationship between the chemical components and the planting area of Corni Fructus, Method: Samples collected from 14 localities of three main production areas of Henan, Zhejiang and Shanxi province were obtained. The contents of main chemical components including loganin, ursolic aicd, polysaccharide, tannins, organic acid, water-soluble extraction and alcohol-soluble extraction were determined to analyze the correlation between chemical components and meteorological factors by PASW STATISTICS 18 software. Result: The results showed the content of ursolic acid was negatively correlated with sunshine hours, polysaccharide content was positively with sunshine hours and average annual temperature, water-soluble extraction was positively correlated with average annual temperature. Conclusion: The results provided theoretical support for good quality cultivation of Corni Fructus.关键词：Corni Fructus;chemical components;meteorological factors;correlation
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- 1
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发布时间：2024-09-23 - 摘要：Objective: To develop a HPLC method for determination of paeoniflorin and limit examination of aconitine in compound Xiaohuoluo pills. Method: For paeoniflorin, C18 column (4.6 mm×150 mm, 5 μm) was used at 30℃; with the mobile phase was acetonitrile-0.4% phosphate solution (14: 86). The detection wavelength was 230 nm and the flow rate wsa set at 1.0 mL·min-1. For aconitine, C18 column (4.6 mm×150 mm, 5 μm) was used at 35℃; with the mobile phase was acetonitrile-tetrahydrofuran(25: 15)and 0.1 mol·L-1 ammonium acetate solution by gradient elution. The detection wavelength was 235 nm and the flow rate wsa set at 0.8 mL·min-1. Result: The linear range of Paeoniflorin was 0.035 5-0.709 6 μg(r=0.999 9).The average recoveries was 101.9% and RSD was 1.86%(n=6). The linear range of aconitine was 0.066 0-0.495 0 μg(r=0.999 8).The average recoveries was 102.0% and RSD was 1.75%(n=6). Conclusion: The method is simple, accurate, stable and can be used for determination of paeoniflorin and limit examination of aconitine in compound Xiaohuoluo pills.关键词：HPLC;paeoniflorin;aconitine;compound Xiaohuoluo pills
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- 1
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发布时间：2024-09-23 - 摘要：Objective:To develop a HPLC method for determination of paeoniflorin and limit examination of aconitine in compound Xiaohuoluo pills.Method: For paeoniflorin,C18 column（4.6 mm×150 mm,5 μm） was used at 30 ℃;with the mobile phase was acetonitrile-0.4% phosphate solution（14∶86）.The detection wavelength was 230 nm and the flow rate wsa set at 1.0 mL · min-1.For aconitine,C18 column（4.6 mm×150 mm,5 μm） was used at 35 ℃;with the mobile phase was acetonitrile-tetrahydrofuran（25∶15）and 0.1 mol·L-1 ammonium acetate solution by gradient elution.The detection wavelength was 235 nm and the flow rate wsa set at 0.8 mL · min-1.Result: The linear range of Paeoniflorin was 0.035 5-0.709 6 μg（r=0.999 9）.The average recoveries was 101.9% and RSD was 1.86%（n=6）.The linear range of aconitine was 0.066 0-0.495 0 μg（r=0.999 8）.The average recoveries was 102.0% and RSD was 1.75%（n=6）.Conclusion: The method is simple,accurate,stable and can be used for determination of paeoniflorin and limit examination of aconitine in compound Xiaohuoluo pills.关键词：HPLC;paeoniflorin;aconitine;compound Xiaohuoluo pills
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- 201
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- 5
发布时间：2024-09-23 - 摘要：Objective:To investigate the chemical constituents from mango seed kernel.Method: The chemical constituents were isolated and purified by silica gel,Sephadex LH-20,preparative TLC and recrystallization.Their structures were identified on the basis of physicochemical property and multiple spectral data.Result: Nine known compounds were obtained from ethyl acetate position,whose structures were elucidated as coumarin（1）,β-sitosterol（2）,vanillin（3）,epicatichin（4）,gallic acid（5）,caffeic acid（6）,ferulic acid（7）,arbutin（8）,ellagic acid（9） respectively.Conclusion: 1-4,6-9 were obtained from mango seed kernel for the first time.关键词：mango seed kernel;crop residues;chemical constituents
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- 265
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- 8
发布时间：2024-09-23 - 摘要：Objective: To investigate the chemical constituents from mango seed kernel. Method: The chemical constituents were isolated and purified by silica gel,Sephadex LH-20,preparative TLC and recrystallization.Their structures were identified on the basis of physicochemical property and multiple spectral data. Result: Nine known compounds were obtained from ethyl acetate position, whose structures were elucidated as coumarin(1),β-sitosterol(2),vanillin(3),epicatichin(4),gallic acid(5),caffeic acid(6),ferulic acid(7),arbutin(8),ellagic acid(9) respectively. Conclusion: 1-4,6-9 were obtained from mango seed kernel for the first time.关键词：mango seed kernel;crop residues;chemical constituents
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发布时间：2024-09-23 - 摘要：Objective: To study the metabolism of combination adiminastration of main components of Gegen Qinlian decoction in rats. Method: The urine samples were collected 0-24 h after ig main components of Gegen Qinlian decoction,then the samples were purified through C18 solid-phase extration cartridge. The purified samples were analyzed by UPLC-Q-TOF-MS. Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses, retention times and full scan metabolisms(MS) spectra with those of the parent drug,and the relative data of authentic reference. Result: Sixteen metabolism were identified as puerarin,daidzin,daidzein,dihydrodaidzein, puerarin 4'-O-β-sulfate, puerarin-7-O-glucuronide, baicalin-6-O-β-glucose-7-O-β-glucopyranuronoside, baicalin-6-O-β-glucopyranuronoside-7-O-β-glucopyranuro-noside, thalifendine, berberrubine,demethyleneberberine,jatrorrhizine,demethyleneberberine-glucuronide,jatrorrhizine-glucuronide, berberrubine-9-O-β-D-glucuronide,demethyleneberberine-2,3-O-β-D-glucuronide. Conclusion: Oxidative demethylenation and subsequent glucuronidation are the major metabolic pathways of combination adiminastration of main components of Gegen Qinlian decoction, some components were mainly excreted unmetabolized in the urine; because of lower bioavailability,first-pass effect in intestine of glycyrrhizic acid,and only a minor part eliminated by urine,the metabolisms of it were not detected.关键词：Gegen Qinlian decoction;metabolisms;UPLC-Q-TOF-MS
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发布时间：2024-09-23 - 摘要：Objective:To study the metabolism of combination adiminastration of main components of Gegen Qinlian decoction in rats.Method: The urine samples were collected 0-24 h after ig main components of Gegen Qinlian decoction,then the samples were purified through C18 solid-phase extration cartridge.The purified samples were analyzed by UPLC-Q-TOF-MS.Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses,retention times and full scan metabolisms（MS） spectra with those of the parent drug,and the relative data of authentic reference.Result: Sixteen metabolism were identified as puerarin,daidzin,daidzein,dihydrodaidzein,puerarin 4′-O-β-sulfate,puerarin-7-O-glucuronide,baicalin-6-O-β-glucose-7-O-β-glucopyranuronoside,baicalin-6-O-β-glucopyranuronoside-7-O-β-glucopyranuro-noside,thalifendine,berberrubine,demethyleneberberine,jatrorrhizine,demethyleneberberine-glucuronide,jatrorrhizine-glucuronide,berberrubine-9-O-β-D-glucuronide,demethyleneberberine-2,3-O-β-D-glucuronide.Conclusion: Oxidative demethylenation and subsequent glucuronidation are the major metabolic pathways of combination adiminastration of main components of Gegen Qinlian decoction,some components were mainly excreted unmetabolized in the urine;because of lower bioavailability,first-pass effect in intestine of glycyrrhizic acid,and only a minor part eliminated by urine,the metabolisms of it were not detected.关键词：Gegen Qinlian decoction;metabolisms;UPLC-Q-TOF-MS
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- 811
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发布时间：2024-09-23 - 摘要：Objective: To investigate the absorption kinetics of 3-n-butylphthalide(NBP) in intestines of rats. Method: The intestine in rat was cannulated for in situ perfusion. UV and HPLC were used to determine the concentration of phenolsulfonphthalein and NBP, respectively. The effect of drug concentration and hydroxypropyl cyclodextrin(HPCD) on the absorption had been studied. Result: When the NBP concentration of perfusion solution was increased, no specific absorption was founded in different segment. The Papp for NBP decreased when HPCD was co-perfused in the intestine. Conclusion: Passive diffusion dominates the absorptive transport behavior of NBP. NBP is well absorbed at all segments of intestine in rats. HPCD can inhibit the absorption of NBP in the intestinal segment.关键词：3-n-butylphthalide;in situ single-pass intestine perfusion model;absorption
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发布时间：2024-09-23 - 摘要：Objective:To investigate the absorption kinetics of 3-n-butylphthalide（NBP） in intestines of rats.Method: The intestine in rat was cannulated for in situ perfusion.UV and HPLC were used to determine the concentration of phenolsulfonphthalein and NBP,respectively.The effect of drug concentration and hydroxypropyl cyclodextrin（HPCD） on the absorption had been studied.Result: When the NBP concentration of perfusion solution was increased,no specific absorption was founded in different segment.The Papp for NBP decreased when HPCD was co-perfused in the intestine.Conclusion: Passive diffusion dominates the absorptive transport behavior of NBP.NBP is well absorbed at all segments of intestine in rats.HPCD can inhibit the absorption of NBP in the intestinal segment.关键词：3-n-butylphthalide;in situ single-pass intestine perfusion model;absorption
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- 179
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Plantaginis Semen extracts（PASEs） on the level of uric acid in hyperuricemia mice and investigate the mechanism.Method:PASEs were administrated to hyperuricemia mice induced by potassium oxonate.The effects of PASEs on serum levels of uric acid,creatinine,and activities of hepatic xanthine oxidase（XOD） and adenosine deaminase（ADA） in hyperuricemia mice were studied.Moreover,The effects of PASEs on renal urate transporter（mURAT1） were analyzed by semi-quantitative RT-PCR method.Result: Different dose groups of observing extract could obviously reduce the level of uric acid,inhibited hepatic xanthine oxidase and denosine deaminase activities in hyperuricemia mice.Furthermore,the PASEs could effectively down-regulate renal mURAT1 level in hyperuricemia mice.And it reversed oxonate-induced elevation in serum creatinine levels of mice.Conclusion:These findings suggested that the PASEs could obviously produce hypourice action by supressing hepatic XOD and ADA to reduce uric acid production and down-regulate renal mURAT1 to decrease urate reabsorption and enhance urate excretion in hyperuricemia mice.关键词：Plantaginis Semen;hyperuricemia;xanthine oxidase;adenosine deaminase;renal urate transporter
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- 1345
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of Plantaginis Semen extracts (PASEs) on the level of uric acid in hyperuricemia mice and investigate the mechanism. Method: PASEs were administrated to hyperuricemia mice induced by potassium oxonate. The effects of PASEs on serum levels of uric acid, creatinine, and activities of hepatic xanthine oxidase (XOD) and adenosine deaminase (ADA) in hyperuricemia mice were studied. Moreover, The effects of PASEs on renal urate transporter (mURAT1) were analyzed by semi-quantitative RT-PCR method. Result: Different dose groups of observing extract could obviously reduce the level of uric acid, inhibited hepatic xanthine oxidase and denosine deaminase activities in hyperuricemia mice. Furthermore, the PASEs could effectively down-regulate renal mURAT1 level in hyperuricemia mice. And it reversed oxonate-induced elevation in serum creatinine levels of mice. Conclusion: These findings suggested that the PASEs could obviously produce hypourice action by supressing hepatic XOD and ADA to reduce uric acid production and down-regulate renal mURAT1 to decrease urate reabsorption and enhance urate excretion in hyperuricemia mice.关键词：Plantaginis Semen;hyperuricemia;xanthine oxidase;adenosine deaminase;renal urate transporter
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of the Shuangyi Jiangtang prescription on the blood glucose and blood lipids in type 2 diabetic rats. Method: rats model of type 2 diabetes mellitus was established by taking high fat diet for 4weeks and combined with STZ ip for 40 mg·kg-1,and the model rats were randomly divided into diabetes model group (T2DM), rosiglitazone maleate positivecontrol groups (RM,ig 0.29 mg·kg-1·d-1), Shuangyi Jiangtang prescription high dose group (SG,ig 4 000 mg·kg-1·d-1), Shuangyi Jiangtang prescription low dose group (SD ig 1 000 mg·kg-1·d-1), and the normal control group (NC). We had observed the general condition of rats during and after the whole treatment of 4 weeks, and tested glucose tolerance, glycated hemoglobin(HbA1c), blood lipids, and fasting serum insulin (INS). Result: In the group of SG, the rats’ symptoms of polydipsia, polyuria and mental status were improved in some degree,fasting blood glucose(FBG), 0.5 h blood glucose(BG) and 2 h BG were lower than the T2DM group,the fasting serum insulin level was higher than the T2DM group (P<0.05). Compared with the model group, fasting blood glucose, glycosylated hemoglobin, lipids were improved in some degree, but there was no significant statistics difference. In the groups of SD and RM, the general condition of rats were bettered, and compared with the model group, the glucose tolerance, HbA1c and the blood lipid of the rats decreased significantly(P<0.05), and the serum insulin increased significantly(P<0.05), but between the two groups of SD and RM, there was no difference in those indexes. Conclusion: The Shuangyi Jiangtang prescrription can improve sugar, lipid metabolism and islet cells function of type 2 diabetic rats,the low-dose group has the best effect, and its efficacy is similar to the rosiglitazone maleate.关键词：Shuangyi Jiangtang prescription;type 2 diabeteas;HbA1c;blood lipids;serum insulin
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of the Shuangyi Jiangtang prescription on the blood glucose and blood lipids in type 2 diabetic rats.Method: rats model of type 2 diabetes mellitus was established by taking high fat diet for 4weeks and combined with STZ ip for 40 mg · kg-1,and the model rats were randomly divided into diabetes model group（T2DM）,rosiglitazone maleate positivecontrol groups（RM,ig 0.29 mg · kg-1 ·d-1）,Shuangyi Jiangtang prescription high dose group（SG,ig 4 000 mg · kg-1 · d-1）,Shuangyi Jiangtang prescription low dose group（SD ig 1 000 mg · kg-1 · d-1）,and the normal control group（NC）.We had observed the general condition of rats during and after the whole treatment of 4 weeks,and tested glucose tolerance,glycated hemoglobin（HbA1c）,blood lipids,and fasting serum insulin（INS）.Result: In the group of SG,the rats’ symptoms of polydipsia,polyuria and mental status were improved in some degree,fasting blood glucose（FBG）,0.5 h blood glucose（BG） and 2 h BG were lower than the T2DM group,the fasting serum insulin level was higher than the T2DM group（P<0.05）.Compared with the model group,fasting blood glucose,glycosylated hemoglobin,lipids were improved in some degree,but there was no significant statistics difference.In the groups of SD and RM,the general condition of rats were bettered,and compared with the model group,the glucose tolerance,HbA1c and the blood lipid of the rats decreased significantly（P<0.05）,and the serum insulin increased significantly（P<0.05）,but between the two groups of SD and RM,there was no difference in those indexes.Conclusion: The Shuangyi Jiangtang prescrription can improve sugar,lipid metabolism and islet cells function of type 2 diabetic rats,the low-dose group has the best effect,and its efficacy is similar to the rosiglitazone maleate.关键词：Shuangyi Jiangtang prescription;type 2 diabeteas;HbA1c;blood lipids;serum insulin
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- 346
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- 12
发布时间：2024-09-23 - 摘要：Objective:To study the effects of Millettia pulcha polysaccharides（YLSP） on neuronal loss and Tau（Ser202） phosphorylation in the frontal lobe and hippocampus of SAMP8 mouse.Method: The SAMP8 mice were adopted as AD model group,and the SAMR1 mice as the control group.Both groups were randomized and given the blank configuration solution.Huperzine（0.02 mg · kg-1） was given to drug-positive group and two YLSP groups were given 45,180 mg · kg-1 respectively,continuous administration for 40 days.In the frontal lobe and hippocampus,neuronal loss and Tau（Ser202） phosphorylation（pser202） were respectively detected by Nissl staining and immunohistochemical method.Result: Comparing with the normal group,the neuronal loss and the number of pSer202 positive cells of the model group were higer（P<0.01） in the frontal lobe and hippocampus.In drug-positive and YLSP groups,the neuronal loss and the number of pSer202 positive cells in the frontal lobe and hippocampus were observed obviously lower（P<0.05 or P<0.01）,comparing with the model group.Conclusion: The abnormal Ser202 phosphorylation is reduced and neuronal loss of brain is ameliorated by YLSP.关键词：Millettia pulcha polysaccharides;SAMP8;Tau phosphorylation;neuronal loss
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- 135
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- 6
发布时间：2024-09-23 - 摘要：Objective: To study the effects of Millettia pulcha polysaccharides(YLSP) on neuronal loss and Tau(Ser202) phosphorylation in the frontal lobe and hippocampus of SAMP8 mouse. Method: The SAMP8 mice were adopted as AD model group, and the SAMR1 mice as the control group. Both groups were randomized and given the blank configuration solution. Huperzine(0.02 mg·kg-1) was given to drug-positive group and two YLSP groups were given 45,180 mg·kg-1 respectively, continuous administration for 40 days. In the frontal lobe and hippocampus, neuronal loss and Tau(Ser202) phosphorylation(pser202) were respectively detected by Nissl staining and immunohistochemical method. Result: Comparing with the normal group, the neuronal loss and the number of pSer202 positive cells of the model group were higer(P<0.01) in the frontal lobe and hippocampus. In drug-positive and YLSP groups, the neuronal loss and the number of pSer202 positive cells in the frontal lobe and hippocampus were observed obviously lower (P<0.05 or P<0.01), comparing with the model group. Conclusion: The abnormal Ser202 phosphorylation is reduced and neuronal loss of brain is ameliorated by YLSP.关键词：Millettia pulcha polysaccharides;SAMP8;Tau phosphorylation;neuronal loss
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- 1
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- 0
发布时间：2024-09-23 - 摘要：Objective:To compare the hepatoprotective effect of Dicliptera chinensi polysaccharide（DCH） with different molecular weight on carbon tetrachloride-induced acute liver injury in rats and its possible mechanism was evaluated.Method:Seventy male Sprague-Dawley rats were randomly divided into model,biphenyl dimethyl dicarboxylate treated group,the total polysaccharide of DCH treated group（Group A）,the polysaccharide of DCH treated groups（Group B,Group C,Group D,which molecular weight is<6 000,6 000-10 000,>10 000）and normal control group.It should be lavaged during 7 day before established the model of Group A（250 mg · kg-1）,Group B（250 mg · kg-1）,Group C（250 mg · kg-1）,Group D（250 mg · kg-1） and biphenyl dimethyl dicarboxylate treated group（250 mg · kg-1）,while other groups were controlled by administration of normal saline.One hour after the final intragastric administration,all rats apart from the rats in the normal control group were lavaged with 0.01% ip carbon-tetrachloride（CCl4） with peanuts oil（10 mL·kg-1） according to establish the model of acute liver injury in rats,while other groups were controlled by administration of normal saline.To detect the ALT,AST and TPA,serum sample were collected after 16 hours by aorta sampling.Result:Compared with the normal control group,the AST,TP and ALT in serum of rats in model group were significantly increased（P<0.01）.The content of TP was reduced by the total polysaccharides of D.chinensis（the A group）（P<0.05 or P<0.01）.The SOD activity was obviously increased by the molecular weight<6 000 group（the B group）（P<0.01）.The GSH-Px activity was increased by the A group and the molecular weight 6 000-10 000 group（the C group）（P<0.01）.Necrosis and passive congestion of hepatic tissue in model group were numerous.The injury of hepatic tissue were inhibited in different polysaccharide of DCH treated groups while the optimum effect is in Group B.The optimum effect of hepatic index appeared in Group D.Conclusion:It was significant that the inhibition of hepatic injury in the polysaccharide of DCH which had different molecular weight treated groups while the effect was the best when molecular weight<6 000.关键词：Dicliptera chinensis;polysaccharide;hepatoprotective effects
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- 284
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发布时间：2024-09-23 - 摘要：Objective: To compare the hepatoprotective effect of Dicliptera chinensi polysaccharide (DCH) with different molecular weight on carbon tetrachloride -induced acute liver injury in rats and its possible mechanism was evaluated. Method: Seventy male Sprague-Dawley rats were randomly divided into model,biphenyl dimethyl dicarboxylate treated group,the total polysaccharide of DCH treated group (Group A),the polysaccharide of DCH treated groups (Group B,Group C,Group D, which molecular weight is<6 000,6 000-10 000,>10 000)and normal control group. It should be lavaged during 7 day before established the model of Group A(250 mg·kg-1),Group B(250 mg·kg-1),Group C(250 mg·kg-1),Group D(250 mg·kg-1) and biphenyl dimethyl dicarboxylate treated group(250 mg·kg-1),while other groups were controlled by administration of normal saline.One hour after the final intragastric administration,all rats apart from the rats in the normal control group were lavaged with 0.01% ip carbon-tetrachloride(CCl4) with peanuts oil(10 mL·kg-1) according to establish the model of acute liver injury in rats,while other groups were controlled by administration of normal saline.To detect the ALT,AST and TPA,serum sample were collected after 16 hours by aorta sampling. Result: Compared with the normal control group,the AST,TP and ALT in serum of rats in model group were significantly increased (P<0.01).The content of TP was reduced by the total polysaccharides of D. chinensis(the A group)(P<0.05 or P<0.01). The SOD activity was obviously increased by the molecular weight<6 000 group(the B group)(P<0.01). The GSH-Px activity was increased by the A group and the molecular weight 6 000-10 000 group(the C group)(P<0.01).Necrosis and passive congestion of hepatic tissue in model group were numerous.The injury of hepatic tissue were inhibited in different polysaccharide of DCH treated groups while the optimum effect is in Group B.The optimum effect of hepatic index appeared in Group D. Conclusion: It was significant that the inhibition of hepatic injury in the polysaccharide of DCH which had different molecular weight treated groups while the effect was the best when molecular weight<6 000.关键词：Dicliptera chinensis;polysaccharide;hepatoprotective effects
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of Zingiber officinale carbo on coagulation function and thromboxane B2（TXB2）,6-keta-prostaglandin F1α（6-keto-PGF1α） in asthenia cold and blood deficiency syndrome Rats.Method: Eighty SD rats were randomly divided into 8 groups: normal control,model,small,medium and large dose groups of Z.officinale carbo.The model of asthenia cold was made by oral administration of rhubarb for 10 days.After 6 days of model,the normal control group and the model group were ig given normal saline.Z.officinale carbo groups were given the Z.officinale carbo decoction at different doses（10,20,40 g · kg-1） daily for 5 days.Blood deficiency syndrome was made by the orbit blooding method.1 h after lasting administration,blood samples were collected to detect the serum levels of thrombin time（TT）,prothrombin time（PT）,activated thromboplastin time（APTT）,fibrinogen（FIB）,d-dimer（D-D） and TXB2/6-keto-PGF1α were observed.Result: Z.officinale carbo could effectively shorten TT（P<0.05）,PT（P<0.05）,APTT（P<0.05）,increase the contents of FIB（P<0.01）,and decrese the contents of D-D（P<0.05）,increase the level of TXB2（P<0.01） and decrease the level of 6-keto-PGF1α（P<0.01）.Conclusion: The mechanism of Z.officinale carbo may affect the coagulation and the level of TXB2 and 6-keto-PGF1α.
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effects of Zingiber officinale carbo on coagulation function and thromboxane B2(TXB2), 6-keta-prostaglandin F1α(6-keto-PGF1α) in asthenia cold and blood deficiency syndrome Rats. Method: Eighty SD rats were randomly divided into 8 groups: normal control,model,small, medium and large dose groups of Z. officinale carbo.The model of asthenia cold was made by oral administration of rhubarb for 10 days.After 6 days of model, the normal control group and the model group were ig given normal saline.Z. officinale carbo groups were given the Z. officinale carbo decoction at different doses (10,20,40 g·kg-1) daily for 5 days.Blood deficiency syndrome was made by the orbit blooding method. 1 h after lasting administration, blood samples were collected to detect the serum levels of thrombin time(TT), prothrombin time(PT), activated thromboplastin time(APTT), fibrinogen(FIB), d-dimer(D-D) and TXB2/6-keto-PGF1α were observed. Result: Z. officinale carbo could effectively shorten TT(P<0.05), PT(P<0.05), APTT(P<0.05), increase the contents of FIB(P<0.01), and decrese the contents of D-D(P<0.05), increase the level of TXB2 (P<0.01) and decrease the level of 6-keto-PGF1α(P<0.01). Conclusion: The mechanism of Z. officinale carbo may affect the coagulation and the level of TXB2 and 6-keto-PGF1α.
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发布时间：2024-09-23 - 摘要：Objective: to investigate the effect of gorgon polysaccharide on the rat’s sports ability and myocardial oxidation resistance ability. Method: Rats were divided into control group and low-dosage, medium-dosage and high-dosage groups.gorgon polysaccharide solution of 100, 200,300 mg·kg-1·d-1 was given for 21 days.The exhaustive swimming time was observed, the content of superoxide dismutase (SOD), malondialdehyde(MDA) and glutathione peroxidase(GSH-Px) was assayed. Result: Gorgon polysaccharide could lengthen the exhaustive swimming time and reduce the content of MDA and increase the content of SOD, MDA and GSH-Px. Conclusion: Gorgon polysaccharide has obvious function on increasing sports ability and myocardial oxidation resistance ability.关键词：gorgon polysaccharide;athletic ability;myocardium;antioxidant capacity
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发布时间：2024-09-23 - 摘要：Objective:to investigate the effect of gorgon polysaccharide on the rat’s sports ability and myocardial oxidation resistance ability.Method: Rats were divided into control group and low-dosage,medium-dosage and high-dosage groups.gorgon polysaccharide solution of 100,200,300 mg · kg-1·d-1 was given for 21 days.The exhaustive swimming time was observed,the content of superoxide dismutase（SOD）,malondialdehyde（MDA） and glutathione peroxidase（GSH-Px） was assayed.Result: Gorgon polysaccharide could lengthen the exhaustive swimming time and reduce the content of MDA and increase the content of SOD,MDA and GSH-Px.Conclusion:Gorgon polysaccharide has obvious function on increasing sports ability and myocardial oxidation resistance ability.关键词：gorgon polysaccharide;athletic ability;myocardium;antioxidant capacity
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发布时间：2024-09-23 - 摘要：Objective: To study the effects of proliferation and apoptosis on K562 cells induced by 17-hydroxy-jolkinolide B (HJB). Method: After the cells were treated with HJB at various concentrations (0, 6.25, 12.5,25,50,100,200,400 μmol·L-1)for 24 h, or at 200 μmol·L-1for various times (12, 24, 48 h), cell viability was measured by 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT). Annexin V-FITC was used to test the apoptosis rate. Absorption spectrometry was adopt to measure the relative activity of Caspase-3 and Caspase-8. Result: The HJB evidently inhibited the growth of K562 cells in a time and dose-dependent manner(P<0.05), with IC50value for 24 h being 158.7 μmol·L-1, and it could induce apoptosis of K562 cells. After HJB treatment at 50, 100, 200 μmol·L-1 for 24 h, the apoptosis rate of K562 cells was 12.14%, 19.26% and 21.78%, respectively(P<0.05),and the relative activity of Caspase-3 or Caspase-8 was advanced with dose-dependent manner. Conclusion: The HJB evidently inhibits the growth of K562 and induces the apoptosis of K562.The mechanism of apoptosis-induced of HJB may be mediated by death receptor pathways.关键词：17-hydroxy-jolkinolide B;K562;cell apoptosis;Caspase-3;caspase-8
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of proliferation and apoptosis on K562 cells induced by 17-hydroxy-jolkinolide B（HJB）.Method:After the cells were treated with HJB at various concentrations（0,6.25,12.5,25,50,100,200,400 μmol · L-1）for 24 h,or at 200 μmol · L-1for various times（12,24,48 h）,cell viability was measured by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）.Annexin V-FITC was used to test the apoptosis rate.Absorption spectrometry was adopt to measure the relative activity of Caspase-3 and Caspase-8.Result:The HJB evidently inhibited the growth of K562 cells in a time and dose-dependent manner（P<0.05）,with IC50value for 24 h being 158.7 μmol·L-1,and it could induce apoptosis of K562 cells.After HJB treatment at 50,100,200 μmol · L-1 for 24 h,the apoptosis rate of K562 cells was 12.14%,19.26% and 21.78%,respectively（P<0.05）,and the relative activity of Caspase-3 or Caspase-8 was advanced with dose-dependent manner.Conclusion: The HJB evidently inhibits the growth of K562 and induces the apoptosis of K562.The mechanism of apoptosis-induced of HJB may be mediated by death receptor pathways.关键词：17-hydroxy-jolkinolide B;K562;cell apoptosis;Caspase-3;caspase-8
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- 148
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发布时间：2024-09-23 - 摘要：Objective: To observe the effects of Shengqing Jiangzhuo Fang(SQJZF) on skeletal muscle in monosodium L-glutamate(MSG) obese rats with insulin resistance. Method: The rat MSG-obese model was produced by giving neonatal rats subcutaneous injection of monosodium glutamate and induced with high-fat diet. Sixty MSG rats were divided into seven grous(4 male and 4 female per gtoup) randomly.the rosiglitazone group,the fenofibrate group,high dose,middle dose and low dose of SQJZF.After the administration for 50 days, levels of cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterin(LDL-C),high density lipoprotein cholesterin(HDL-C) and glucogn in muscle tissue were measured. Result: SQJZF showed good effects on the animal models. Compared with the model group,treatment with SQJZF at a dose of 6,3,1.5 g·kg-1 for 50 d decreased levels of TC,TG,LDL-C and glucogn in muscle tissue (P<0.01);levels of HDL-C was significantly elvated by treatment with SQJZF at a dose of 3 g·kg-1(P<0.01). Conclusion: SQJZF may contribute to ameliorate the glucolipid metablization in diabetic skeletal muscle.关键词：Shengqing Jiangzhuo Fang;MSG;obese rat;IR;skeletal muscle.
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Shengqing Jiangzhuo Fang（SQJZF） on skeletal muscle in monosodium L-glutamate（MSG） obese rats with insulin resistance.Method: The rat MSG-obese model was produced by giving neonatal rats subcutaneous injection of monosodium glutamate and induced with high-fat diet.Sixty MSG rats were divided into seven grous（4 male and 4 female per gtoup） randomly.the rosiglitazone group,the fenofibrate group,high dose,middle dose and low dose of SQJZF.After the administration for 50 days,levels of cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterin（LDL-C）,high density lipoprotein cholesterin（HDL-C） and glucogn in muscle tissue were measured.Result:SQJZF showed good effects on the animal models.Compared with the model group,treatment with SQJZF at a dose of 6,3,1.5 g · kg-1 for 50 d decreased levels of TC,TG,LDL-C and glucogn in muscle tissue（P<0.01）;levels of HDL-C was significantly elvated by treatment with SQJZF at a dose of 3 g · kg-1（P<0.01）.Conclusion:SQJZF may contribute to ameliorate the glucolipid metablization in diabetic skeletal muscle.关键词：Shengqing Jiangzhuo Fang;MSG;obese rat;IR;skeletal muscle.
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发布时间：2024-09-23 - 摘要：Objective: To explore the anti-oxidation effects of daidzein (DD)on rat's myocardial hypertrophy caused by isopropylarterenol (Iso). Method: The rat's myocardial hypertrophy model was formed by hypodermic injection of Iso 1 mg·kg-1)for days at back skin,DD (0.1, 0.3 μmol·kg-1),dimethylsulfoxide(DMSO) or normal saline(NS) were administrated in intragastric way from the second day for 14 days. After the last administration, 12 hours after fasting blood and heart were collected The indexes of whole heart and left ventricle were calculated,superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)activity and malondialdehyde (MDA) concentration in serum were measured. Result: It showed that the indexes of whole heart and left ventriclein Iso group were much bigger than control one,the activity of SOD and GSH-Px in serum was decreased, and the concentration of MDA in serum enhanced. However, DD improved these indexes. Conclusion: DD has a protection on rat's myocardial hypertrophy caused by Iso through enhancing the anti-oxidation activity of rats.关键词：daidzin;isopropylarterenol;myocardial hypertrophy;anti-oxidation
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发布时间：2024-09-23 - 摘要：Objective:To explore the anti-oxidation effects of daidzein（DD）on rat’s myocardial hypertrophy caused by isopropylarterenol（Iso）.Method: The rat’s myocardial hypertrophy model was formed by hypodermic injection of Iso 1 mg · kg-1)for days at back skin,DD（0.1,0.3 μmol · kg-1）,dimethylsulfoxide（DMSO） or normal saline（NS） were administrated in intragastric way from the second day for 14 days.After the last administration,12 hours after fasting blood and heart were collected The indexes of whole heart and left ventricle were calculated,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）activity and malondialdehyde（MDA） concentration in serum were measured.Result:It showed that the indexes of whole heart and left ventriclein Iso group were much bigger than control one,the activity of SOD and GSH-Px in serum was decreased,and the concentration of MDA in serum enhanced.However,DD improved these indexes.Conclusion: DD has a protection on rat’s myocardial hypertrophy caused by Iso through enhancing the anti-oxidation activity of rats.关键词：daidzin;isopropylarterenol;myocardial hypertrophy;anti-oxidation
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- 150
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发布时间：2024-09-23 - 摘要：Objective: We explore a practical feed formulation in order to establish a stable atherosclerosis animal model.With high fat and hyperhomocysteinemia in shorter period. Method: Forty male SD rats were divided into 4 groups (n=10), given different formula feeding with common feed,formulationⅠ(not containing propylthiouracil),formulation II(containing propylthiouracil of 0.15%) and formulation Ⅲ(containing propylthiouracil of 0.25%). weighed once a week.The blood samples were collected at week 2 and 4 respectively, then to determine the levels of total cholesterol(TC),low density lipoprotein cholesterin(LDL-C) in serum and homocysteine(Hcy) in plasma. Result: Compared with the control group, weight of formulationⅠhad no significant difference, while weight of formulation II and formulation Ⅲ decreased significantly (P<0.01);TC,LDL-C and Hcy levels in formulation II and formulation Ⅲ groups increased significantly (P<0.01) at 2, 4 weeks,respectively,but formulationⅠhave no significant difference. Conclusion: In this study,we found that the rats fed with the formulation II can establish a stable atherosclerosis animal model with high fat and hyperhomocysteinemia in shorter period.关键词：atherosclerosis;blood lipid;Hcy
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发布时间：2024-09-23 - 摘要：Objective:We explore a practical feed formulation in order to establish a stable atherosclerosis animal model.With high fat and hyperhomocysteinemia in shorter period.Method:Forty male SD rats were divided into 4 groups（n=10）,given different formula feeding with common feed,formulationⅠ（not containing propylthiouracil）,formulation II（containing propylthiouracil of 0.15%） and formulation Ⅲ（containing propylthiouracil of 0.25%）.weighed once a week.The blood samples were collected at week 2 and 4 respectively,then to determine the levels of total cholesterol（TC）,low density lipoprotein cholesterin（LDL-C） in serum and homocysteine（Hcy） in plasma.Result: Compared with the control group,weight of formulationⅠhad no significant difference,while weight of formulation II and formulation Ⅲ decreased significantly（P<0.01）;TC,LDL-C and Hcy levels in formulation II and formulation Ⅲ groups increased significantly（P<0.01） at 2,4 weeks,respectively,but formulationⅠhave no significant difference.Conclusion: In this study,we found that the rats fed with the formulation II can establish a stable atherosclerosis animal model with high fat and hyperhomocysteinemia in shorter period.关键词：atherosclerosis;blood lipid;Hcy
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发布时间：2024-09-23 - 摘要：Objective:To explore the molecular mechanisms of Purendan superfine powder（PRD）on metabolic disorder and the protection on macroangiopathy by investigating the protein expression of insulin receptor substrate-1（IRS-1）,phosphatidylinositol 3-kinase（PI3K）,glucose transporter 4（GLUT4） and nuclear factor-κB（NF-κB） of skeletal muscles in type 2 diabetes mellitus（T2DM） rats with macroangiopathy.Method: High-fat-diet/streptozotocin（STZ,30 mg · kg-1）-induced type 2 diabetes mellitus rats were developed and treated with PRD（0.885,1.770,3.540 g · kg-1） for four weeks and rosiglitazone（0.36×10-3 g · kg-1） was used as positive control drugs,then western blot assay was used to analyze the protein expression of IRS-1,PI3K,GLUT 4 and NF-κB of skeletal muscle in the rats.Result: The protein expression of IRS-1,PI3K and GLUT-4 was significantly decreased in T2DM group compared with the control group（P<0.01）,while NF-κB expression was significantly increased（P<0.01）.After the treatment of PRD,the expression of IRS-1,PI3K and GLUT-4 was up-regulated,and NF-κB was down-regulated significantly（P<0.05 or P<0.01）,which was equivalent to the positive drugs.Conclusion: PRD could improve insulin resistance,decrease blood glucose and fat,protect the vascular endothelium injury by up-regulating IRS-1,PI-3K and GLUT 4 protein expression and down-regulating NF-κB protein expression of skeletal muscle in T2DM rats.关键词：PRD;T2DM;IRS-1;GLUT 4;PI3K;NF-κB
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发布时间：2024-09-23 - 摘要：Objective: To explore the molecular mechanisms of Purendan superfine powder(PRD)on metabolic disorder and the protection on macroangiopathy by investigating the protein expression of insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase (PI3K), glucose transporter 4 (GLUT4) and nuclear factor-κB (NF-κB) of skeletal muscles in type 2 diabetes mellitus(T2DM) rats with macroangiopathy. Method: High-fat-diet/streptozotocin (STZ, 30 mg·kg-1)-induced type 2 diabetes mellitus rats were developed and treated with PRD (0.885,1.770,3.540 g·kg-1) for four weeks and rosiglitazone (0.36×10-3 g·kg-1) was used as positive control drugs, then western blot assay was used to analyze the protein expression of IRS-1, PI3K, GLUT 4 and NF-κB of skeletal muscle in the rats. Result: The protein expression of IRS-1, PI3K and GLUT-4 was significantly decreased in T2DM group compared with the control group (P<0.01), while NF-κB expression was significantly increased (P<0.01). After the treatment of PRD, the expression of IRS-1, PI3K and GLUT-4 was up-regulated, and NF-κB was down-regulated significantly (P<0.05 or P<0.01), which was equivalent to the positive drugs. Conclusion: PRD could improve insulin resistance, decrease blood glucose and fat, protect the vascular endothelium injury by up-regulating IRS-1, PI-3K and GLUT 4 protein expression and down-regulating NF-κB protein expression of skeletal muscle in T2DM rats.关键词：PRD;T2DM;IRS-1;GLUT 4;PI3K;NF-κB
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发布时间：2024-09-23 - 摘要：Objective: To identify the effect of rat serum containing different concentrations of Buzhong Yiqi decoction(BZYQT) on reverse the chemotherapeutic resistance in A549/DDP cells and on the expression of mTOR. Method: Animals were randomized to prepared the rat serum containing varied does levels of BZYQT(does for 11.34,5.67,2.83 g·kg-1·d-1),the A549,A549/DDP were equally divided into six groups in random: the DDP group, BZYQT low does+DDP group, BZYQT middle does+DDP group, BZYQT high does+DDP group, NS+DDP group and the cell control group.Followed by 15%serum of every group and the different concentrations of DDP(10,20,40,80,100,200 μmol·L-1) for 48 h, assayed by MTT to detect the rat serum containing of BZYQT reverse the chemotherapeutic resistance in A549/DDP.The expression of mTOR in cells were evaluated by indirect immunofluorescence, immunocytochemistry technique and Western-blot. Result: The median inhibitory concentration IC50 and resistant multiple of A549/DDP is significant reduction by the serum containing varied does levels of BZYQT(P<0.05),especially the effect of the middle dose group is the most significant(P<0.01),the optical density and fluorescence intensity of mTOR were significantly reduced(P<0.01) and the protein expression levels were significantly lower in the cells(P<0.01). Conclusion: BZYQT appeared to increase the chemotherapeutic sensitivity of A549/DDP cells, showing regression of multiple chemotherapeutic resistance in pulmonary adenocarcinoma by down-regulating expression levels of mTOR.关键词：Buzhong Yiqi Decoction;tumor;A549/DDP cells;mTOR
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发布时间：2024-09-23 - 摘要：Objective:To identify the effect of rat serum containing different concentrations of Buzhong Yiqi decoction（BZYQT） on reverse the chemotherapeutic resistance in A549/DDP cells and on the expression of mTOR.Method: Animals were randomized to prepared the rat serum containing varied does levels of BZYQT（does for 11.34,5.67,2.83 g · kg-1 · d-1）,the A549,A549/DDP were equally divided into six groups in random: the DDP group,BZYQT low does+DDP group,BZYQT middle does+DDP group,BZYQT high does+DDP group,NS+DDP group and the cell control group.Followed by 15%serum of every group and the different concentrations of DDP（10,20,40,80,100,200 μmol · L-1） for 48 h,assayed by MTT to detect the rat serum containing of BZYQT reverse the chemotherapeutic resistance in A549/DDP.The expression of mTOR in cells were evaluated by indirect immunofluorescence,immunocytochemistry technique and Western-blot.Result: The median inhibitory concentration IC50 and resistant multiple of A549/DDP is significant reduction by the serum containing varied does levels of BZYQT（P<0.05）,especially the effect of the middle dose group is the most significant（P<0.01）,the optical density and fluorescence intensity of mTOR were significantly reduced（P<0.01） and the protein expression levels were significantly lower in the cells（P<0.01）.Conclusion: BZYQT appeared to increase the chemotherapeutic sensitivity of A549/DDP cells,showing regression of multiple chemotherapeutic resistance in pulmonary adenocarcinoma by down-regulating expression levels of mTOR.关键词：Buzhong Yiqi Decoction;tumor;A549/DDP cells;mTOR
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发布时间：2024-09-23 - 摘要：Objective: To evaluate the mechanism of licochalcone B induce mouse melanoma B16F0 cells apoptosis. Method: Effect of licochalcone B on proliferation of B16F0 cells was determined by MTT. Hoechst 33258 fluorescent staining were used to observe the morphological changes of apoptotic cell. Apoptosis was determined by staining cells with annexin V fluorescein isothiocyanate (FITC) and propidium iodide (PI) labeling.The mRNA expression levels of B cell lymphoma/lewkmia-2(Bcl-2) associated X protein(Bax) and Bcl-2 were detected using RT-PCR analysis. Changes of Caspase-9 and Caspase-3 were measured with fluorescence assay. Result: Licochalcone B(5-15 mg·L-1) can effectively inhibit B16F0 cell proliferation in a dose-dependent manner. IC50 was 11.3 mg·L-1, and cell obviously appeared apoptosis characteristics, with the concentration of licochalcone B increased, the apoptosis rate was increased, licochalcone B(10, 12.5 mg·L-1) significantly lowered the mRNA expression levels of Bcl-2/Bax, and raised the expression of caspase-9 and caspase-3, licochaleone B(7.5 mg·L-1) cause the activation degree of Caspase-9 and Caspase-3 higher than 12.5 mg·L-1. Conclusion: Licochalcone B induce B16F0 cell apoptosis through mitochondria-dependent pathway.
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the mechanism of licochalcone B induce mouse melanoma B16F0 cells apoptosis.Method: Effect of licochalcone B on proliferation of B16F0 cells was determined by MTT.Hoechst 33258 fluorescent staining were used to observe the morphological changes of apoptotic cell.Apoptosis was determined by staining cells with annexin V fluorescein isothiocyanate（FITC） and propidium iodide（PI） labeling.The mRNA expression levels of B cell lymphoma/lewkmia-2（Bcl-2） associated X protein（Bax） and Bcl-2 were detected using RT-PCR analysis.Changes of Caspase-9 and Caspase-3 were measured with fluorescence assay.Result: Licochalcone B（5-15 mg·L-1） can effectively inhibit B16F0 cell proliferation in a dose-dependent manner.IC50 was 11.3 mg·L-1,and cell obviously appeared apoptosis characteristics,with the concentration of licochalcone B increased,the apoptosis rate was increased,licochalcone B（10,12.5 mg·L-1） significantly lowered the mRNA expression levels of Bcl-2/Bax,and raised the expression of caspase-9 and caspase-3,licochaleone B（7.5 mg·L-1） cause the activation degree of Caspase-9 and Caspase-3 higher than 12.5 mg·L-1.Conclusion: Licochalcone B induce B16F0 cell apoptosis through mitochondria-dependent pathway.
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of Zhengan Xifeng decoction (ZGXFD) on pathological changes and neuron apoptosis in cerebral tissuein of middle cerebral artery occlusion(MACO) in rats. The effect of ZGXFD on the behavior obstade in rats was observed. Method: The model of focal cerebral ischemia in rats was established with the suture-occuluded method and was used to intervene with different dose of ZGXFD (15,30 g·kg-1,respectively)before MCAO. The effect of ZGXFD on the behavior obstacle in rats was observed, the infarct size and the pathological change were observed by TTC staining, and HE dyeing,respectively. TUNEL was used to evaluate the apoptosis of neurons, and Western blot was used to observe the expression of Caspase 3 and poly ADP-ribose polymerase(PARP). Result: After modeling, behavior obstacle with different degree appeared in all rats. The infarct size could be observed in all groups except sham group. The nerve cell apoptosis could be found in all rats after 6 h of MCAO. The result showed that the ZGXFT improved the behavior obstacle, reduced the infarct size and cerebral edema obviously;and the AI and apoptosis of Caspase-3 and PARP were decreased significantly after 6 h MCAO. Conclusion: ZGXFD pretreatment has obvious protective effects on the focal cerebral ischemia in rats.关键词：Zhengan Xifeng decoction (ZGXFD);middle cerebral artery occlusion (MCAO);TUNEL;Caspase-3;PARP
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Zhengan Xifeng decoction（ZGXFD） on pathological changes and neuron apoptosis in cerebral tissuein of middle cerebral artery occlusion（MACO） in rats.The effect of ZGXFD on the behavior obstade in rats was observed.Method: The model of focal cerebral ischemia in rats was established with the suture-occuluded method and was used to intervene with different dose of ZGXFD（15,30 g ·kg-1,respectively）before MCAO.The effect of ZGXFD on the behavior obstacle in rats was observed,the infarct size and the pathological change were observed by TTC staining,and HE dyeing,respectively.TUNEL was used to evaluate the apoptosis of neurons,and Western blot was used to observe the expression of Caspase 3 and poly ADP-ribose polymerase（PARP）.Result: After modeling,behavior obstacle with different degree appeared in all rats.The infarct size could be observed in all groups except sham group.The nerve cell apoptosis could be found in all rats after 6 h of MCAO.The result showed that the ZGXFT improved the behavior obstacle,reduced the infarct size and cerebral edema obviously;and the AI and apoptosis of Caspase-3 and PARP were decreased significantly after 6 h MCAO.Conclusion: ZGXFD pretreatment has obvious protective effects on the focal cerebral ischemia in rats.关键词：Zhengan Xifeng decoction（ZGXFD）;middle cerebral artery occlusion（MCAO）;TUNEL;Caspase-3;PARP
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of saikosaponin a（SSa） on expression of multidrug resistant protein P-glycoprotein（P-gp） in refractory epilepsy rats induced by licl-pilocarpine.Method: Except for the blank control group（A）,thirty SD refractory epilepsy rats were randomly divided into 5 groups,namely,sodium valproate（VPA） group C（200 mg · kg-1,ig）,low,middle and high dose of SSa group（0.54,1.09,2.18 mg · kg-1,ip）.The model group（B） and group A received the same amount of saline.At the end of treatment for 8 weeks,the expression of P-gp in rat hippocampus and temporal lobe cortex was detected by Western blotting.Result: The P-gp expressed in the rat hippocampus and temporal cortex in different groups had statistically significant difference（P<0.001）.Compared with group A,the expression of P-gp were increased significantly in the other groups（P<0.05）;the expression was increased in group C（P<0.05）,but decreased in group D,E and F when compared with group B.The reduction of expression was most apparent in group E（P<0.05）.Conclusion:SSa can reduce the expression of P-gp in hippocampus and temporal cortex with a slightly dose-dependent manner,which may be one of the anticonvulsant mechanisms of SSa.关键词：refractory epilepsy;saikosaponin A;multiple drug resistance;P-glycoprotein
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of saikosaponin a(SSa) on expression of multidrug resistant protein P-glycoprotein(P-gp) in refractory epilepsy rats induced by licl-pilocarpine. Method: Except for the blank control group (A),thirty SD refractory epilepsy rats were randomly divided into 5 groups, namely,sodium valproate(VPA) group C (200 mg·kg-1,ig),low,middle and high dose of SSa group(0.54, 1.09, 2.18 mg·kg-1,ip). The model group (B) and group A received the same amount of saline. At the end of treatment for 8 weeks, the expression of P-gp in rat hippocampus and temporal lobe cortex was detected by Western blotting. Result: The P-gp expressed in the rat hippocampus and temporal cortex in different groups had statistically significant difference(P<0.001). Compared with group A, the expression of P-gp were increased significantly in the other groups(P<0.05);the expression was increased in group C(P<0.05),but decreased in group D,E and F when compared with group B. The reduction of expression was most apparent in group E(P<0.05). Conclusion: SSa can reduce the expression of P-gp in hippocampus and temporal cortex with a slightly dose-dependent manner,which may be one of the anticonvulsant mechanisms of SSa.关键词：refractory epilepsy;saikosaponin A;multiple drug resistance;P-glycoprotein
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发布时间：2024-09-23 - 摘要：Objective:The goal of the present study is to explore the interactions between Wutou Tang and CYP3A4 enzyme by determining the influence of Wutou tang on the activity of CPY3A4 in rat liver microsomes.Method: Fifty male SD rats were randomly divided into five groups.3 groups were intragastrically treated with Wutou tang high dose（2 times of the original prescription dose）,medium dose（original prescription dose） and low dose（0.5 times of the original prescription dose） for 7 consecutive days,respectively.Drinking water was given intragastrically to blank control group and SKF-525A（0.1 g · kg-1） was given intragastrically to inhibitory control group.The activity of CPY3A4 was determined by UPLC method using testosterone as probe in vitro.Result: Compared with the blank control group,the testosterone metabolic rates were significantly lower of groups which were treated with Wutou tang high dose,medium dose and low dose（P<0.05）.The testosterone metabolic rates of Wutou tang high dose group was lower than Wutou tang medium dose group and low dose group.Conclusion: The activity of CPY3A4 was significantly inhibited with the continuous use of Wutou tang,which indicates that taking Wutou tang with CYP3A4 enzyme substrates might cause drug interactions.关键词：Wutou tang;testosterone;UPLC;CYP3A4
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- 467
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发布时间：2024-09-23 - 摘要：Objective: The goal of the present study is to explore the interactions between Wutou Tang and CYP3A4 enzyme by determining the influence of Wutou tang on the activity of CPY3A4 in rat liver microsomes. Method: Fifty male SD rats were randomly divided into five groups. 3 groups were intragastrically treated with Wutou tang high dose (2 times of the original prescription dose), medium dose (original prescription dose) and low dose (0.5 times of the original prescription dose) for 7 consecutive days, respectively. Drinking water was given intragastrically to blank control group and SKF-525A (0.1 g·kg-1) was given intragastrically to inhibitory control group. The activity of CPY3A4 was determined by UPLC method using testosterone as probe in vitro. Result: Compared with the blank control group, the testosterone metabolic rates were significantly lower of groups which were treated with Wutou tang high dose, medium dose and low dose (P<0.05). The testosterone metabolic rates of Wutou tang high dose group was lower than Wutou tang medium dose group and low dose group. Conclusion: The activity of CPY3A4 was significantly inhibited with the continuous use of Wutou tang, which indicates that taking Wutou tang with CYP3A4 enzyme substrates might cause drug interactions.关键词：Wutou tang;testosterone;UPLC;CYP3A4
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发布时间：2024-09-23 - 摘要：Objective:To investigate the influence of artesunate（ART） on invasion and apoptosis of novel human palatal salivary gland cell line of adenoid cystic carcinoma（NACC）.Method: After treated with ART 100 mg · L-1,apoptosis cells were observed after loaded with acridine orange/ethidium bromide dye（AO/EB）.The anti-invasion effect of ART at 25,50,100 mg · L-1 were examined by transwell assay.The expression of vascular endothelial growth factor（VEGF） and its mRNA of NACC cells was evaluated by immunohistochemistry and RT-qPCR technique separately.Result:①Compared with the control group,apoptosis cells were observed after treated with ART 100 mg · L-1,with irregular and shrinked nucleolus;②The result of transwell assay showed that ART at 25,50,100 mg · L-1 could inhibit the transmembrane cells obviously compared with control group（P<0.01）;③Compared with the control group,the VEGF protein expression decreased obviously after stimulated with ART（P<0.01）;④Same result were observed in VEGF mRNA expression by RT-qPCR（P<0.01）.Conclusion: ART could induce apoptosis,decrease VEGF expression and invasion of NACC cells in vitro.关键词：artesunate;adenoid cystic carcinoma;apoptosis;invasion;VEGF
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发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of artesunate (ART) on invasion and apoptosis of novel human palatal salivary gland cell line of adenoid cystic carcinoma (NACC). Method: After treated with ART 100 mg·L-1, apoptosis cells were observed after loaded with acridine orange/ethidium bromide dye (AO/EB). The anti-invasion effect of ART at 25,50,100 mg·L-1 were examined by transwell assay. The expression of vascular endothelial growth factor (VEGF) and its mRNA of NACC cells was evaluated by immunohistochemistry and RT-qPCR technique separately. Result: ①Compared with the control group, apoptosis cells were observed after treated with ART 100 mg·L-1, with irregular and shrinked nucleolus; ②The result of transwell assay showed that ART at 25, 50, 100 mg·L-1 could inhibit the transmembrane cells obviously compared with control group(P<0.01); ③Compared with the control group, the VEGF protein expression decreased obviously after stimulated with ART (P<0.01); ④Same result were observed in VEGF mRNA expression by RT-qPCR (P<0.01). Conclusion: ART could induce apoptosis, decrease VEGF expression and invasion of NACC cells in vitro.关键词：artesunate;adenoid cystic carcinoma;apoptosis;invasion;VEGF
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发布时间：2024-09-23 - 摘要：Objective: To study effect of different technologic extracts from shengzhi Yingyang fang on renal function poisoned by adenine in mice. Method: One hundred SPF grade male healthy mice were randomly divided into 5 groups, which were the control group, the model group, Shengzhi Yingyang Fang 1 group(water extraction), Shengzhi Yingyang Fang 2 group(75% alcohol), Shengzhi Yingyang Fang 3 group(40% alcohol). The models were established by orally giving adenine to the mice at 300 mg·kg-1 and at the same time, 3 experimental groups were orally given different technologic extracts from sheng zhiyingyang fang decoctum (crude drugs at 1.96 g·kg-1), five times a week for 2 months. Result: Shengzhi Yingyang fang significantly decreased (P<0.05)the contents of creatinine (SCr),urea nitrogen(BUN),lactic acid(LD) and the activity of xanthine oxidase(XOD)in plasma. Furthermore, it could significantly reduce(P<0.05) the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) and improve(P<0.05)the activities of succinodehydrogenase (SDH) of renal tissue poisoned by adenine in mice. The effect of water extraction is the best. Conclusion: Different technologic extracts from shengzhi Yingyang fang decoctum have protective effects on renal function poisoned by adenine, among which the effect of water extract is the best.关键词：shengzhi Yingyang fang;adenine;kidney
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发布时间：2024-09-23 - 摘要：Objective:To study effect of different technologic extracts from shengzhi Yingyang fang on renal function poisoned by adenine in mice.Method: One hundred SPF grade male healthy mice were randomly divided into 5 groups,which were the control group,the model group,Shengzhi Yingyang Fang 1 group（water extraction）,Shengzhi Yingyang Fang 2 group（75% alcohol）,Shengzhi Yingyang Fang 3 group（40% alcohol）.The models were established by orally giving adenine to the mice at 300 mg · kg-1 and at the same time,3 experimental groups were orally given different technologic extracts from sheng zhiyingyang fang decoctum（crude drugs at 1.96 g · kg-1）,five times a week for 2 months.Result: Shengzhi Yingyang fang significantly decreased（P<0.05）the contents of creatinine（SCr）,urea nitrogen（BUN）,lactic acid（LD） and the activity of xanthine oxidase（XOD）in plasma.Furthermore,it could significantly reduce（P<0.05） the activities of acid phosphatase（ACP） and alkaline phosphatase（AKP） and improve（P<0.05）the activities of succinodehydrogenase（SDH） of renal tissue poisoned by adenine in mice.The effect of water extraction is the best.Conclusion: Different technologic extracts from shengzhi Yingyang fang decoctum have protective effects on renal function poisoned by adenine,among which the effect of water extract is the best.关键词：shengzhi Yingyang fang;adenine;kidney
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- 103
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发布时间：2024-09-23 - 摘要：Objective: To observe the therapeutic effects of Rhamnus Songorica extract (RSE) in rats with alcoholic liver disease. Method: A rat model of alcoholic liver disease was established by intragastric infusion of alcohol, once a day continuously for 10 weeks. Positive control group was given yiganling at 100 mg·kg-1 and the low, middle, high dose RSE groups were given RSE at 0.9, 1.8, 3.6 g·kg-1, respectively after 10 weeks. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) in serum, maleic dialdehyde (MDA), total superoxide dismutase (T-SOD), nitric oxide (NO), reduced glutathione (GSH) in liver tissue and the evaluation of the histopathological changes of liver in rats were detected after 4 weeks treatment. Result: Compared with the rats in model group, RSE significantly decreased the levels of ALT, AST, TG, TC, and LDL-C in serum as well as MDA and NO in liver tissue. The T-SOD conten was obviously increased in liver tissue. The hepatic pathological changes were milder in the RSE groups than in the model group. Conclusion: RSE has obviously therapeutic effects on alcoholic liver disease in rat. The mechanisms may be relative to reducing lipid peroxide and protecting the structure of hepatocyte via enhancing the abilities of the free radical scavenging and antilipoperoxidant activities.关键词：alcoholic liver disease;rhamnus songorica extract;oxidative stress;lipid peroxide;free radial;antilipoperoxidant
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发布时间：2024-09-23 - 摘要：Objective:To observe the therapeutic effects of Rhamnus Songorica extract（RSE） in rats with alcoholic liver disease.Method: A rat model of alcoholic liver disease was established by intragastric infusion of alcohol,once a day continuously for 10 weeks.Positive control group was given yiganling at 100 mg · kg-1 and the low,middle,high dose RSE groups were given RSE at 0.9,1.8,3.6 g · kg-1,respectively after 10 weeks.The levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,triglyceride（TG）,total cholesterol（TC）,low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C） in serum,maleic dialdehyde（MDA）,total superoxide dismutase（T-SOD）,nitric oxide（NO）,reduced glutathione（GSH） in liver tissue and the evaluation of the histopathological changes of liver in rats were detected after 4 weeks treatment.Result: Compared with the rats in model group,RSE significantly decreased the levels of ALT,AST,TG,TC,and LDL-C in serum as well as MDA and NO in liver tissue.The T-SOD conten was obviously increased in liver tissue.The hepatic pathological changes were milder in the RSE groups than in the model group.Conclusion: RSE has obviously therapeutic effects on alcoholic liver disease in rat.The mechanisms may be relative to reducing lipid peroxide and protecting the structure of hepatocyte via enhancing the abilities of the free radical scavenging and antilipoperoxidant activities.关键词：alcoholic liver disease;rhamnus songorica extract;oxidative stress;lipid peroxide;free radial;antilipoperoxidant
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- 108
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发布时间：2024-09-23 - 摘要：Objective: To investigate the benefitial and protective effects of Jili Liangdi Tang (JLLDT) on Streptozotocin (STZ)-induced diabetes mellitus and periphery neuropathic pain. Method: Sixty rats were employed and divided into control, model, positive control (metformin 200 mg·kg-1) and JLLDT low, medium and high dose (16.2, 32.4, 64.8 g·kg-1) groups evenly. Except of the control group, all the rats were established type II diabetes mellitus induced by intraperitoneal injection of STZ. The rats were intragastric administration of corresponding agent for 4 weeks, while the intake of food and water were recorded. The blood glucose and insulin levels were also measured by blood glucose meter or ELISA kit. Sixty rats were employed and divided into control, model, positive control (gabapentin 120 mg·kg-1) and JLLDT low, medium and high dose (16.2, 32.4, 64.8 g·kg-1) groups evenly. Except of control group, all the rats were established diabetes mellitus neuropathy induced by intravenous injection of STZ for 7 days. The rats were intragastric administration of corresponding drugs, while the thermal hyperalgesia and mechanical allodynia were measured respectively. Result: JLLDT could decrease the food and water intake and elevate the body weight of diabetic rats significantly in a dose-dependent manner. The blood glucose concentration was deceased to 10.3 mmol·L-1and insulin level was elevated to 0.61 μg·L-1significantly after administration of JLLDT high dose. The three doses of JLLDT could ameliorate the thermal hyperagesia and allodynia of model rats respectively. The analygesia rate in JLLDT high dose reached at 50.3% and 63.2%, respectively (P<0.05). Conclusion: JLLDT exhibits pronounced ability of decrease diabetic blood glucose by elevating insulin secretion, and has protective effect in diabetic periphery neuropathic pain.关键词：Jili Liangdi Tang;diabetes mellitus;peripheral neuropathic pain
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发布时间：2024-09-23 - 摘要：Objective:To investigate the benefitial and protective effects of Jili Liangdi Tang（JLLDT） on Streptozotocin（STZ）-induced diabetes mellitus and periphery neuropathic pain.Method: Sixty rats were employed and divided into control,model,positive control（metformin 200 mg · kg-1） and JLLDT low,medium and high dose（16.2,32.4,64.8 g · kg-1） groups evenly.Except of the control group,all the rats were established type II diabetes mellitus induced by intraperitoneal injection of STZ.The rats were intragastric administration of corresponding agent for 4 weeks,while the intake of food and water were recorded.The blood glucose and insulin levels were also measured by blood glucose meter or ELISA kit.Sixty rats were employed and divided into control,model,positive control（gabapentin 120 mg · kg-1） and JLLDT low,medium and high dose（16.2,32.4,64.8 g · kg-1） groups evenly.Except of control group,all the rats were established diabetes mellitus neuropathy induced by intravenous injection of STZ for 7 days.The rats were intragastric administration of corresponding drugs,while the thermal hyperalgesia and mechanical allodynia were measured respectively.Result: JLLDT could decrease the food and water intake and elevate the body weight of diabetic rats significantly in a dose-dependent manner.The blood glucose concentration was deceased to 10.3 mmol · L-1and insulin level was elevated to 0.61 μg · L-1significantly after administration of JLLDT high dose.The three doses of JLLDT could ameliorate the thermal hyperagesia and allodynia of model rats respectively.The analygesia rate in JLLDT high dose reached at 50.3% and 63.2%,respectively（P<0.05）.Conclusion: JLLDT exhibits pronounced ability of decrease diabetic blood glucose by elevating insulin secretion,and has protective effect in diabetic periphery neuropathic pain.关键词：Jili Liangdi Tang;diabetes mellitus;peripheral neuropathic pain
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发布时间：2024-09-23 - 摘要：Objective: To analyze the rationality of Sangi fang. Method: Rats were divided randomly into control group, Sanqi fang group(0.767 g·kg-1), Sanqi fang without Ziziphi Spinesae Semen group(0.517 g·kg-1), Sanqi fang without Spatholobi Caulis group(0.433 g·kg-1), Sanqi fang without Notoginseng Radix et Rhizoma group(0.683 g·kg-1) and Sanqi fang without Cirsii Herba group (0.667 g·kg-1). All of rats in therapeutic groups were administered the medication onally for 11 days and the rats in blank control group were given the distilled water with same volume. On the 11th day, the behavior test was performed including spontaneous movement and cooperating function with Pelltobarbitalum Natricum. Result: Compared to the control group, Sanqi fang and Sanqigang without Spatholobi Caulis group could remarkably reduce the degree of rat's spontaneous movement(P<0.05); Sanqi fang group could increase the sleeping rate significantly(P<0.05). While both Sanqi fang without Spatholobi Caulis and without Cirsii Herba group could increase the tendency of sleeping rate. Sanqi fang goup(P<0.05) and Sanqi fang without Sanqi group(P<0.01) could reduce fall-sleep time significantly, while Sanqi fang without Cirsii Herba group had the tendency of reduce fall-sleep time. Sanqi fang group, Sanqi fang without Cirsii Herba group and Sanqi fang without Notoginseng Radix et Rhizoma group can increase sleeping time of rats significantly(P<0.05). Conclusion: Sanqi fang has sedative-hypnotics effect, and its the prescription formula is reasonable.关键词：insomnia;sedative-hypnotics;Sanqi fang;prescription research
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发布时间：2024-09-23 - 摘要：Objective:To analyze the rationality of Sangi fang.Method: Rats were divided randomly into control group,Sanqi fang group（0.767 g · kg-1）,Sanqi fang without Ziziphi Spinesae Semen group（0.517 g · kg-1）,Sanqi fang without Spatholobi Caulis group（0.433 g · kg-1）,Sanqi fang without Notoginseng Radix et Rhizoma group（0.683 g · kg-1） and Sanqi fang without Cirsii Herba group（0.667 g · kg-1）.All of rats in therapeutic groups were administered the medication onally for 11 days and the rats in blank control group were given the distilled water with same volume.On the 11th day,the behavior test was performed including spontaneous movement and cooperating function with Pelltobarbitalum Natricum.Result: Compared to the control group,Sanqi fang and Sanqigang without Spatholobi Caulis group could remarkably reduce the degree of rat’s spontaneous movement（P<0.05）;Sanqi fang group could increase the sleeping rate significantly（P<0.05）.While both Sanqi fang without Spatholobi Caulis and without Cirsii Herba group could increase the tendency of sleeping rate.Sanqi fang goup（P<0.05） and Sanqi fang without Sanqi group（P<0.01） could reduce fall-sleep time significantly,while Sanqi fang without Cirsii Herba group had the tendency of reduce fall-sleep time.Sanqi fang group,Sanqi fang without Cirsii Herba group and Sanqi fang without Notoginseng Radix et Rhizoma group can increase sleeping time of rats significantly（P<0.05）.Conclusion: Sanqi fang has sedative-hypnotics effect,and its the prescription formula is reasonable.关键词：insomnia;sedative-hypnotics;Sanqi fang;prescription research
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of aqueous extract of Polygala tenuifolia(APT) on myocardial contractility and atrial natriuretic peptide (ANP) secretion in beating rabbit atria. Method: The APT-induced changes in atrial dynamics, cAMP efflux and ANP secretion were determined in perfused beating rabbit atria. The changes of cAMP and ANP were measured by radioimmunoassay. Result: APT increased atrial stroke volume, pulse pressure and cAMP efflux in a concentration-dependent manner with inhibition of ANP secretion in beating rabbit atria. The APT-induced positive inotropic effect was significantly blocked by pretreatment with blockade of β and β1-adrenoceptors (AR) (P<0.05) but not β2-AR. Furthermore, the APT-induced changes in myocardial contractility were attenuated by inhibition of L-type Ca2+channel and protein kinase A(PKA) activation with no changes in cAMP efflux. Conclusion: APT could increase atrial dynamics via β1-AR-cAMP-Ca2+signaling and decreases ANP secretion in beating rabbit atria.关键词：Polygala tenuifolia;myocardial contractility;ANP secretion;β1-AR-cAMP-Ca2+signaling pathway
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of aqueous extract of Polygala tenuifolia（APT） on myocardial contractility and atrial natriuretic peptide（ANP） secretion in beating rabbit atria.Method: The APT-induced changes in atrial dynamics,cAMP efflux and ANP secretion were determined in perfused beating rabbit atria.The changes of cAMP and ANP were measured by radioimmunoassay.Result: APT increased atrial stroke volume,pulse pressure and cAMP efflux in a concentration-dependent manner with inhibition of ANP secretion in beating rabbit atria.The APT-induced positive inotropic effect was significantly blocked by pretreatment with blockade of β and β1-adrenoceptors（AR）（P<0.05） but not β2-AR.Furthermore,the APT-induced changes in myocardial contractility were attenuated by inhibition of L-type Ca2+channel and protein kinase A（PKA） activation with no changes in cAMP efflux.Conclusion: APT could increase atrial dynamics via β1-AR-cAMP-Ca2+signaling and decreases ANP secretion in beating rabbit atria.关键词：Polygala tenuifolia;myocardial contractility;ANP secretion;β1-AR-cAMP-Ca2+signaling pathway
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- 212
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发布时间：2024-09-23 - 摘要：Objective:To study the anti-invation effct of triptolide for U87 glioma cells and its molecular mechanism.Method: MTT assay was used to determine the growth inhibition by triptolide in glioma U87 cells.Tumor invasion and migration were examined by Transwell chamber assay and wound-healing method.Real-time PCR was conducted to detect alteration of matrix metallopeptidase 9（MMP-9） and Cathepsins B（CB）.The alteration of nuclear factor kappa B（NF-κB） p65 expression were studied by Western blot.Result: Triptolide could significantly inhibite glioma U87 cell proliferation,reduce the cell ability of migration and invasion,down-regulating its MMP-9,CB and NF-κB p65 expression level.Conclusion: Triptolide could depress the invasion of glioma cells owing to down-regulating the level of NF-κB pathway which could inhibit MMP-9 activities,reduce the ability of hydrolyzing extracellular matrix in vitro.关键词：triptolide;glioma;U87 cells;invasion;nuclear factor kappa B;callepsins B
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发布时间：2024-09-23 - 摘要：Objective: To study the anti-invation effct of triptolide for U87 glioma cells and its molecular mechanism. Method: MTT assay was used to determine the growth inhibition by triptolide in glioma U87 cells. Tumor invasion and migration were examined by Transwell chamber assay and wound-healing method. Real-time PCR was conducted to detect alteration of matrix metallopeptidase 9 (MMP-9) and Cathepsins B (CB). The alteration of nuclear factor kappa B (NF-κB) p65 expression were studied by Western blot. Result: Triptolide could significantly inhibite glioma U87 cell proliferation, reduce the cell ability of migration and invasion, down-regulating its MMP-9, CB and NF-κB p65 expression level. Conclusion: Triptolide could depress the invasion of glioma cells owing to down-regulating the level of NF-κB pathway which could inhibit MMP-9 activities, reduce the ability of hydrolyzing extracellular matrix in vitro.关键词：triptolide;glioma;U87 cells;invasion;nuclear factor kappa B;callepsins B
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of Huanglian Jiedu Tang (HJT) in the reversal effect of drug resistance in Escherichia coli. Method: E. coli with extended-spectrum β-lactamase is screened by disc diffusion method. The minimum inhibitory concentration of HJT was determined.The extended-spectrum β-lactamase enzyme E. coli was cultured with a HJT concentration lower than the minimum inhibitory concentration. The cultivation duration and optimal concentration for reversal effect were selected, and the reversal rate was determined. Result: Minimum inhibitory concentration of HJT to the E. coli ATCC8099 was 0.6 g·L-1. The cultivation duration was 30 h and the optimal concentration for reversal effect was 0.3 g·L-1.The reversal rate of ampicillin was 60.0%. The reversal rate of ceftazidime was 66.7%. The reversal rate of cefmetazole was 75.0%. The reversal rate of ceftriaxone was 66.7%. Conclusion: The effect of HJT for reversal drug resistance in E. coli is defined. It is a way to reverse drug resistance in E. coli.关键词：Huanglian Jiedu Tang;drug resistance;E. coli;reversal effect
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Huanglian Jiedu Tang（HJT） in the reversal effect of drug resistance in Escherichia coli.Method: E.coli with extended-spectrum β-lactamase is screened by disc diffusion method.The minimum inhibitory concentration of HJT was determined.The extended-spectrum β-lactamase enzyme E.coli was cultured with a HJT concentration lower than the minimum inhibitory concentration.The cultivation duration and optimal concentration for reversal effect were selected,and the reversal rate was determined.Result: Minimum inhibitory concentration of HJT to the E.coli ATCC8099 was 0.6 g · L-1.The cultivation duration was 30 h and the optimal concentration for reversal effect was 0.3 g · L-1.The reversal rate of ampicillin was 60.0%.The reversal rate of ceftazidime was 66.7%.The reversal rate of cefmetazole was 75.0%.The reversal rate of ceftriaxone was 66.7%.Conclusion: The effect of HJT for reversal drug resistance in E.coli is defined.It is a way to reverse drug resistance in E.coli.关键词：Huanglian Jiedu Tang;drug resistance;E.coli;reversal effect
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- 275
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the inhibitory activity of Sanqing Jiangtang decoction and chemical constituents on alpha glucosidase enzyme and to exploit the bioactive constituents.Method: The samples were prepared by macroporous adsorptive resins（JT-1-JT-13）.Glucose oxidase method and starch tolerance test experiments were adopted to evaluate the inhibitory activity of Sanqing Jiangtang decoction and its different separated fractions.Result: It was found that fractions（JT-1,JT-3,JT-6,JT-7）,separated from Sanqing Jiangtang decoction,mainly contributed to the inhibitory activity.JT-7 showed the most significant activities in the experiments.Conclusion:Glycosides and alkaloid may be the principle effective substance in the active fraction.关键词：Sanqing Jiangtang decoction;α-glucosidase enzyme;effective substance
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- 141
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发布时间：2024-09-23 - 摘要：Objective: To evaluate the inhibitory activity of Sanqing Jiangtang decoction and chemical constituents on alpha glucosidase enzyme and to exploit the bioactive constituents. Method: The samples were prepared by macroporous adsorptive resins(JT-1-JT-13). Glucose oxidase method and starch tolerance test experiments were adopted to evaluate the inhibitory activity of Sanqing Jiangtang decoction and its different separated fractions. Result: It was found that fractions(JT-1,JT-3,JT-6,JT-7),separated from Sanqing Jiangtang decoction,mainly contributed to the inhibitory activity. JT-7 showed the most significant activities in the experiments. Conclusion: Glycosides and alkaloid may be the principle effective substance in the active fraction.关键词：Sanqing Jiangtang decoction;α-glucosidase enzyme;effective substance
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发布时间：2024-09-23 - 摘要：Objective: To study anti-inflammatory and analgesic effect of water extract of Sauropus rostratus. Method: The LD50level of the water extract was determined by modified Karber's method. Dimethylbenzene-induced auricle edema in mice,carrageenan-induced hind paw edema in rats and cotton ball-induced granuloma in mice were respectively performed to investigate the anti-inflammatory effect of the extract (14,7,3.5 g·kg-1). Mouse twisting numbers induced by acetic acid and mouse pain threshold after heat stimulation on hot-plate tests were respectively performed to investigate the analgesic effect of S. rostratus. Result: The LD50level of water extract of S. rostratus. was 68 g·kg-1, the auricle edema induced by dimethylbenzene in mice,catton ball-induced granuloma in mice and carrageenan-induced hind paw edema in rats were inhibited significantly by water extract of S. rostratus. (P<0.05 or P<0.01), water extract of S. rostratus could remarkably reduce mouse twisting numbers (P<0.05 or P<0.01), but could not improve pain threshold. Conclusion: The water extract of S. rostratus had anti-inflammatory effect and analgesic effect,the researches could provide a scientific basis for developing and utilizing of S. rostratus.关键词：Sauropus rostratus;anti-inflammatory effect;analgesic effect
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发布时间：2024-09-23 - 摘要：Objective:To study anti-inflammatory and analgesic effect of water extract of Sauropus rostratus.Method: The LD50level of the water extract was determined by modified Karber’s method.Dimethylbenzene-induced auricle edema in mice,carrageenan-induced hind paw edema in rats and cotton ball-induced granuloma in mice were respectively performed to investigate the anti-inflammatory effect of the extract（14,7,3.5 g · kg-1）.Mouse twisting numbers induced by acetic acid and mouse pain threshold after heat stimulation on hot-plate tests were respectively performed to investigate the analgesic effect of S.rostratus.Result:The LD50level of water extract of S.rostratus.was 68 g · kg-1,the auricle edema induced by dimethylbenzene in mice,catton ball-induced granuloma in mice and carrageenan-induced hind paw edema in rats were inhibited significantly by water extract of S.rostratus.（P<0.05 or P<0.01）,water extract of S.rostratus could remarkably reduce mouse twisting numbers（P<0.05 or P<0.01）,but could not improve pain threshold.Conclusion:The water extract of S.rostratus had anti-inflammatory effect and analgesic effect,the researches could provide a scientific basis for developing and utilizing of S.rostratus.关键词：Sauropus rostratus;anti-inflammatory effect;analgesic effect
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发布时间：2024-09-23 - 摘要：Objective: To study the effects of Cuscuta chinensis on free radical in brain tissue and ability of resistance in exercise-induced fatigue rats. Method: The high-intensity endurance training in rats was used to establish exercise-induced fatigue model.The modeling rats were divided into the 4 groups:model group, low,medium and high groups of C. chinensis group (1.16, 2.32, 6.96 g·kg-1·d-1, ig). Another group without exercise as a control group was set after 6 weeks of exhaustive swimming training. 24 h after the last training, weight, exhaustive swimming time and malondialdehyde(MDA), superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in brain were observed. Result: The weight in C. chinensis groups was increased group(P<0.05), swimming time became longer (P<0.01) compared with model.In C. chinensis groups MDA were decreased compared with model group (P<0.01), and SOD, GSH-Px activity was significantly increasee. Conclusion: C. chinensis has the antioxidation action and increase ability of resistance on exercise-induced fatigue关键词：Cuscuta chinensis;anti-fatigue;brain;free radical
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of Cuscuta chinensis on free radical in brain tissue and ability of resistance in exercise-induced fatigue rats.Method: The high-intensity endurance training in rats was used to establish exercise-induced fatigue model.The modeling rats were divided into the 4 groups:model group,low,medium and high groups of C.chinensis group（1.16,2.32,6.96 g · kg-1 · d-1,ig）.Another group without exercise as a control group was set after 6 weeks of exhaustive swimming training.24 h after the last training,weight,exhaustive swimming time and malondialdehyde（MDA）,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in brain were observed.Result: The weight in C.chinensis groups was increased group（P<0.05）,swimming time became longer（P<0.01） compared with model.In C.chinensis groups MDA were decreased compared with model group（P<0.01）,and SOD,GSH-Px activity was significantly increasee.Conclusion: C.chinensis has the antioxidation action and increase ability of resistance on exercise-induced fatigue关键词：Cuscuta chinensis;anti-fatigue;brain;free radical
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发布时间：2024-09-23 - 摘要：Objective: To observe effect of astragalosides extract on liver fibrosis in rats. Method: Sixty SD rats were randomly divided into six groups: normal control group (A), model group (B), colchicine group(C),low-dose astragalosides group(D),middle-dose astragalosides group (E) and high-dose astragalosides group (F).Liver fibrosis model in rat was produced with bovine serum albumin(ABS). Group C received colchicine (0.1 mg·kg-1·d-1);group D,E, F received low-dose astragalosides (15 mg·kg-1·d-1), middle-dose astragalosides (30 mg·kg-1·d-1) and high-dose astragalosides (60 mg·kg-1·d-1) ig respectively. After 55 days, serum levels of serum total protein (TP), albumin(ALB),alanine aminotransferase(ALT),aspartate aminotransferase(AST),hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PC-Ⅲ) and type Ⅳ collagen (Ⅳ-C) in different groups were detected. Result: Compared with model group,levels of ALT,AST,HA,LN,PC-Ⅲand Ⅳ-C in the treatment groups were obviously improved.It had significantly statistical difference(P<0.05, P<0.01).Pathological result showed that the liver fibrosis in the treatment groups was signiflcantly attanuated. Conclusion: These results suggested that astragalosides shows obvious effect against rat hepatic fibrosis.关键词：Astragalosides;liver fibrosis;bovine serum albumin
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发布时间：2024-09-23 - 摘要：Objective:To observe effect of astragalosides extract on liver fibrosis in rats.Method: Sixty SD rats were randomly divided into six groups: normal control group（A）,model group（B）,colchicine group（C）,low-dose astragalosides group（D）,middle-dose astragalosides group（E） and high-dose astragalosides group（F）.Liver fibrosis model in rat was produced with bovine serum albumin（ABS）.Group C received colchicine（0.1 mg · kg-1 · d-1）;group D,E,F received low-dose astragalosides（15 mg · kg-1 · d-1）,middle-dose astragalosides（30 mg·kg-1·d-1） and high-dose astragalosides（60 mg · kg-1 · d-1） ig respectively.After 55 days,serum levels of serum total protein（TP）,albumin（ALB）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,hyaluronic acid（HA）,laminin（LN）,type Ⅲ procollagen（PC-Ⅲ） and type Ⅳ collagen（Ⅳ-C） in different groups were detected.Result: Compared with model group,levels of ALT,AST,HA,LN,PC-Ⅲand Ⅳ-C in the treatment groups were obviously improved.It had significantly statistical difference（P<0.05,P<0.01）.Pathological result showed that the liver fibrosis in the treatment groups was signiflcantly attanuated.Conclusion: These results suggested that astragalosides shows obvious effect against rat hepatic fibrosis.关键词：Astragalosides;liver fibrosis;bovine serum albumin
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发布时间：2024-09-23 - 摘要：Objective: To study Didang decoction on effect of tumor necrosis factor-α (TNF-α) and interleukin-6(IL-6), immunoglobulin G(IgG) levels of tissue homogenate in chronic prostatitis rats. Method: Divide 50 rats into 5 groups,the black contrast group(distilled water 10 mL·kg-1),model contrast group(distilled water 10 mL·kg-1),Didang decoction high-dose(16.2 g·kg-1) and low-dose(8.1 g·kg-1) groups,Yang contrast group(Qian liekang 0.5 g·kg-1).The black contrast group was injected with Nacl into prostates by 0.1 mL.The other groups were injected with Xiaozhiling into the prostates by 0.1 mL.All of the groups were injected the stomach with the medicines one time everyday at the same time when models had been made.Testing tissue homogenate TNF-α,IL-6,IgG after 5 weeks. Result: Comparying with the model contrast group, tissue homogenate TNF-α,IL-6,IgG(67.63±15.15),(56.43±23.75),(23.26±2.57)ng·L-1, other 4 groups were significantly lower(P<0.05). Comparying with the Yang contrast group, tissue homogenate TNF-α,IgG(37.90±13.64), (19.08±2.47) ng·L-1,Didang decoction high-dose group (23.88±8.18),(15.94±1.24) ng·L-1,is significantly descreased (P<0.05).Comparying with Didang decoction low-dose group,tissue homogenate TNF-α,IgG(39.80±11.12),(19.22±1.11)ng·L-1,Didang decoction high-dose group is significantly descreased (P<0.05). Conclusion: Didang decoction can improve the immune function,inhibiting the inflammatory reaction.关键词：chronic prostatitis;Didang Decoction;TNF-α;IL-6;IgG
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发布时间：2024-09-23 - 摘要：Objective:To study Didang decoction on effect of tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6）,immunoglobulin G（IgG） levels of tissue homogenate in chronic prostatitis rats.Method:Divide 50 rats into 5 groups,the black contrast group（distilled water 10 mL · kg-1）,model contrast group（distilled water 10 mL · kg-1）,Didang decoction high-dose（16.2 g · kg-1） and low-dose（8.1 g · kg-1） groups,Yang contrast group（Qian liekang 0.5 g · kg-1）.The black contrast group was injected with Nacl into prostates by 0.1 mL.The other groups were injected with Xiaozhiling into the prostates by 0.1 mL.All of the groups were injected the stomach with the medicines one time everyday at the same time when models had been made.Testing tissue homogenate TNF-α,IL-6,IgG after 5 weeks.Result: Comparying with the model contrast group,tissue homogenate TNF-α,IL-6,IgG（67.63±15.15）,（56.43±23.75）,（23.26±2.57）ng · L-1,other 4 groups were significantly lower（P<0.05）.Comparying with the Yang contrast group,tissue homogenate TNF-α,IgG（37.90±13.64）,（19.08±2.47） ng · L-1,Didang decoction high-dose group（23.88±8.18）,（15.94±1.24） ng · L-1,is significantly descreased（P<0.05）.Comparying with Didang decoction low-dose group,tissue homogenate TNF-α,IgG（39.80±11.12）,（19.22±1.11）ng · L-1,Didang decoction high-dose group is significantly descreased（P<0.05）.Conclusion: Didang decoction can improve the immune function,inhibiting the inflammatory reaction.关键词：chronic prostatitis;Didang Decoction;TNF-α;IL-6;IgG
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Shenxiong Huayu capsule preconditioning on the expression of vascular endothelial growth factor（VEGF） after cerebral ischemia reperfusion injury in rats,to investigate the protective effect of shenxiong Huayu capsule on acute cerebral ischemia reperfusion injury in rats.Method:The rats were randomly divided into five groups:sham operatiion group,ischemia reperfusion group,Shenxiong Huayu capsule high,medium,low dose preconditioning group（480,240,120 mg · kg-1）.The group were divided randomly into 3,6,12,24,48,72 h group after the 2 hour focal cerebral ischemia reperfusion（n=6）.Intragastric administration 7 day,once a day.Middle cerebral artery occlusion（MCAO） and reperfushion model was made by an intraluminal filament method.The expressions of VEGF proteins were detected by using immunohistochemical method,the neurological deficit score was assessed at the same time.Result:Compared with the sham operation group,severe neurologic deficits of rats were presented and increases of VEGF in the ischemia reperfusion group at every time point（3,6,12,24,48,72 h）,and showing a significant difference（P<0.05,or P<0.01）.Compared with the ischemia reperfusion group,neurologic deficits of rats were relieved significantly and VEGF expression increased distinctly in Shenxiong Huayu capsule dose preconditioning group at every time point（3,6,12,24,48,72 h）,showing a significant difference（P<0.05,or P<0.01）,high dose group showing best significant.Conclusion:Shenxiong Huayu Capsule may relive acute cerebral ischemia reperfusion injury in rats by increasing VEGF expression.关键词：cerebral ischemia-reperfusion;vascular endothelial growth factor;Shenxiong-Huayu capsule
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发布时间：2024-09-23 - 摘要：Objective: To observe the effect of Shenxiong Huayu capsule preconditioning on the expression of vascular endothelial growth factor(VEGF) after cerebral ischemia reperfusion injury in rats, to investigate the protective effect of shenxiong Huayu capsule on acute cerebral ischemia reperfusion injury in rats. Method: The rats were randomly divided into five groups:sham operatiion group, ischemia reperfusion group, Shenxiong Huayu capsule high, medium,low dose preconditioning group(480,240,120 mg·kg-1). The group were divided randomly into 3, 6, 12, 24, 48, 72 h group after the 2 hour focal cerebral ischemia reperfusion(n=6). Intragastric administration 7 day, once a day. Middle cerebral artery occlusion (MCAO) and reperfushion model was made by an intraluminal filament method. The expressions of VEGF proteins were detected by using immunohistochemical method, the neurological deficit score was assessed at the same time. Result: Compared with the sham operation group, severe neurologic deficits of rats were presented and increases of VEGF in the ischemia reperfusion group at every time point(3, 6,12,24,48,72 h), and showing a significant difference(P<0.05, or P<0.01). Compared with the ischemia reperfusion group, neurologic deficits of rats were relieved significantly and VEGF expression increased distinctly in Shenxiong Huayu capsule dose preconditioning group at every time point(3,6,12,24,48, 72 h), showing a significant difference(P<0.05, or P<0.01), high dose group showing best significant. Conclusion: Shenxiong Huayu Capsule may relive acute cerebral ischemia reperfusion injury in rats by increasing VEGF expression.关键词：cerebral ischemia-reperfusion;vascular endothelial growth factor;Shenxiong-Huayu capsule
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发布时间：2024-09-23 - 摘要：Objective: To explore the effect of Simo decoction on gastrointestinal function and brain-gut peptide in serum of patients with non-gastrointestinal postoperative. Method: To use randomized, double-blind and double-dummy study.72 cases of gynecologic tumor surgery were randomly divided into observation group and control group. The observation group was given Simo decoction and domperidone placebo,control group was given domperidone and Simo decoction placebo. Observed postoperative recovery of gastrointestinal function and serum cholecystobinin(CCK) and leptin level changes after treated 5 days. Result: The postoperative bowel sound recovery time and first flatus,defecation time in observation group significantly earlier than the control group, the differences were statistically significant (P<0.05 or P<0.01). After treatment,the level of serum CCK and leptin declined in observation group, the difference was also statistically significant(P<0.05 or P<0.01) compared with before treatment. Conclusion: Simo decoction can promote non-gastrointestinal postoperative recovery of gastrointestinal function,it may be associated with the regulation of serum CCK and leptin levels change.关键词：Simo decoction;non-gastrointestinal postoperative;gastrointestinal function;cholecystobinin;leptin
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发布时间：2024-09-23 - 摘要：Objective:To explore the effect of Simo decoction on gastrointestinal function and brain-gut peptide in serum of patients with non-gastrointestinal postoperative.Method:To use randomized,double-blind and double-dummy study.72 cases of gynecologic tumor surgery were randomly divided into observation group and control group.The observation group was given Simo decoction and domperidone placebo,control group was given domperidone and Simo decoction placebo.Observed postoperative recovery of gastrointestinal function and serum cholecystobinin（CCK） and leptin level changes after treated 5 days.Result:The postoperative bowel sound recovery time and first flatus,defecation time in observation group significantly earlier than the control group,the differences were statistically significant（P<0.05 or P<0.01）.After treatment,the level of serum CCK and leptin declined in observation group,the difference was also statistically significant（P<0.05 or P<0.01） compared with before treatment.Conclusion:Simo decoction can promote non-gastrointestinal postoperative recovery of gastrointestinal function,it may be associated with the regulation of serum CCK and leptin levels change.关键词：Simo decoction;non-gastrointestinal postoperative;gastrointestinal function;cholecystobinin;leptin
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发布时间：2024-09-23 - 摘要：Objective: To observe the efficacy of tiaoqichanglong decoction of Zhuang medicine in the treatment of overweight and obesity. Method: The treatment group was treated with the Tiaoqi Changlong decoction of Zhuang medicine.And the control group was given the routine Western medication. Result: The effective rate in treatment group was 92%, improving the obesity indications was better than control group. Conclusion: Tiaoqi Changlong decoction of Zhuang medicine has a good effect on overweight and obesity.关键词：Zhuang medicine;decoction;overweight;obesity
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发布时间：2024-09-23 - 摘要：Objective:To observe the efficacy of tiaoqichanglong decoction of Zhuang medicine in the treatment of overweight and obesity.Method: The treatment group was treated with the Tiaoqi Changlong decoction of Zhuang medicine.And the control group was given the routine Western medication.Result: The effective rate in treatment group was 92%,improving the obesity indications was better than control group.Conclusion: Tiaoqi Changlong decoction of Zhuang medicine has a good effect on overweight and obesity.关键词：Zhuang medicine;decoction;overweight;obesity
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发布时间：2024-09-23 - 摘要：Objective: The aim of this study are to evaluate the clinical effect and toxicities of cocurrent nedaplatin chemoradiotherapy with intensity modulated radiotherapy (IMRT)plus adjuvant chemotherapy for locally advanced nasopharyngeal carcinoma. Method: A total of 60 patients with locally advanced nasopharyngeal carcinoma(according to Fuzhou 92 staging system)were enrolled into this study,40 patients had stage Ⅲ lesion while 20 patients had stage Ⅳa lesion.The regions of nasopharynx and upper and lower neck were all irradiated by IMRT technique. The prescribed dose 69.96 Gy was delivered to the gross tumor volume (PTV) and positive neck nodes (PTVnd); 59.36 Gy to the clinical target volume (PTV1), covering the upper neck and area around the nasopharynx; and 50.96 Gy to the low neck and supraclavicular area (PTV2). The dose to 50% of the parotid was ≤35 Gy. The maximum dose to the lens, pituitary gland, temporo-mandibular joint, mandible, and temporal lobe was 9, 54, 60, 70, 60 Gy. The maximum dose to the brain stem, spinal cord, optic nerve and optic chiasma (PRV) was 54, 40, 54, 54 Gy, respectively.Acute toxicities were evaluated according to EORTC/RTOG radiation morbidity scoring criteria.All patients received concurre chemoradiotherapy before three cycles of adjuvant chemotherapy. Result: With a median follow-up of 56 months,the 1,2,3,4-year locoregional control rate was 100% and 98.33%,93.3%,90.0%, the 1,2,3,4-year overall survival and distant metastasis free survival were 98.33%,98.33%,90.00%,85.0%and 96.67%,93.33%,86.7%,80.0%respectively. Conclusion: Cocurrent chemoradiotherapy with IMRT plus adjuvant chemotherapy for locally advanced nasopharyngeal carcinoma is feasible,grade 3 to 4 mucositis and grade 3 to 4 hematologic toxicity are considered to be limiting factors of chemoradiotherapy and influence the quality of life.It may be able to improve the locoregional control rate and overall survival for locally advanced nasopharyngeal carcinoma and be worthy of further clinical investigation.关键词：nasopharyngeal carcinoma;intensity modulated radiotherapy;concurrent radiochemotherapy;adjuvant chemotherapy;nedaplatin
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发布时间：2024-09-23 - 摘要：Objective:The aim of this study are to evaluate the clinical effect and toxicities of cocurrent nedaplatin chemoradiotherapy with intensity modulated radiotherapy（IMRT）plus adjuvant chemotherapy for locally advanced nasopharyngeal carcinoma Method:A total of 60 patients with locally advanced nasopharyngeal carcinoma（according to Fuzhou 92 staging system）were enrolled into this study,40 patients had stage Ⅲ lesion while 20 patients had stage Ⅳa lesion.The regions of nasopharynx and upper and lower neck were all irradiated by IMRT technique.The prescribed dose 69.96 Gy was delivered to the gross tumor volume（PTV） and positive neck nodes（PTVnd）;59.36 Gy to the clinical target volume（PTV1）,covering the upper neck and area around the nasopharynx;and 50.96 Gy to the low neck and supraclavicular area（PTV2）.The dose to 50% of the parotid was ≤35 Gy.The maximum dose to the lens,pituitary gland,temporo-mandibular joint,mandible,and temporal lobe was 9,54,60,70,60 Gy.The maximum dose to the brain stem,spinal cord,optic nerve and optic chiasma（PRV） was 54,40,54,54 Gy,respectively.Acute toxicities were evaluated according to EORTC/RTOG radiation morbidity scoring criteria.All patients received concurre chemoradiotherapy before three cycles of adjuvant chemotherapy.Result: With a median follow-up of 56 months,the 1,2,3,4-year locoregional control rate was 100% and 98.33%,93.3%,90.0%,the 1,2,3,4-year overall survival and distant metastasis free survival were 98.33%,98.33%,90.00%,85.0%and 96.67%,93.33%,86.7%,80.0%respectively.Conclusion: Cocurrent chemoradiotherapy with IMRT plus adjuvant chemotherapy for locally advanced nasopharyngeal carcinoma is feasible,grade 3 to 4 mucositis and grade 3 to 4 hematologic toxicity are considered to be limiting factors of chemoradiotherapy and influence the quality of life.It may be able to improve the locoregional control rate and overall survival for locally advanced nasopharyngeal carcinoma and be worthy of further clinical investigation.关键词：nasopharyngeal carcinoma;intensity modulated radiotherapy;concurrent radiochemotherapy;adjuvant chemotherapy;nedaplatin
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发布时间：2024-09-23 - 摘要：Objective: To observe the effect of the therapeutic principle of Waisan Neixi on C-reactive protein in patients with stroke. Method: One hundred and twenty-two stroke patients with acute phase wind syndrome were randomly divided into the control group and the observation group. The two groups were treated by basic intervention for 14-28 d. In addition to the basic intervention, the control group was adopted a simple Pinggan Xifeng treatment by the prescription of Tianma Goutengyin. While the observation group was received the therapeutic principle of Waisan Neixi with Shuangjie Tang. The Drink and the Tang were given one dose daily for 14 days. Result: Compared with the control group, the observation group showed early significant improvement on the Wind Syndrome, and decreased the level of C-reactive protein(CRP)(P<0.05). Conclusion: Stroke patients in the acute phase with wind syndrome are affected by the external wind factors. The therapeutic principle of Waisan Neixi can improve the scores of wind syndrome,and reduce the inflammatory response at early stage.关键词：stroke;wind syndrome;the therapeutic principle of Waisan Neixi;CRP
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of the therapeutic principle of Waisan Neixi on C-reactive protein in patients with stroke.Method: One hundred and twenty-two stroke patients with acute phase wind syndrome were randomly divided into the control group and the observation group.The two groups were treated by basic intervention for 14-28 d.In addition to the basic intervention,the control group was adopted a simple Pinggan Xifeng treatment by the prescription of Tianma Goutengyin.While the observation group was received the therapeutic principle of Waisan Neixi with Shuangjie Tang.The Drink and the Tang were given one dose daily for 14 days.Result: Compared with the control group,the observation group showed early significant improvement on the Wind Syndrome,and decreased the level of C-reactive protein（CRP）（P<0.05）.Conclusion: Stroke patients in the acute phase with wind syndrome are affected by the external wind factors.The therapeutic principle of Waisan Neixi can improve the scores of wind syndrome,and reduce the inflammatory response at early stage.关键词：stroke;wind syndrome;the therapeutic principle of Waisan Neixi;CRP
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发布时间：2024-09-23 - 摘要：Objective:To observe the Bufei granules in the treatment of asthma in remission stage effect.Method: Fourty-three cases of asthma patients in remission,were randomly divided into a treatment group（22 cases）,21 cases of the control group.The treatment group given Bufei particles,the control group was given Bufei particle simulation agent.Treatment for 1 month,followed up for 3 months.Observation: asthma symptoms integral,asthma control test（ACT） score,indices of pulmonary function,blood eosinophil count index.Using IBM SPSS Statistics 19 statistical analysis.Result: Traditional Chinese Medicine（TCM） syndrome scoring,the treatment group decreased obviously,compared with the control group（P<0.01） has significant difference;patients with ACT score,the treatment group increased significantly,（P<0.01） has significant difference;and can reduce the peripheral blood eosinophil count,and before treatment is compared to P<0.05,have the difference of statistics;improved peak expiratory flow（PEF）,with significant difference,（P<0.01）;reduce attack frequency.Conclusion: Bufei granules can obviously improve the symptoms of patients with bronchial asthma in remission stage,and improve lung function,reduce the level of eosinophils（EOS）,through the inhibition of airway inflammation,and to control the remission stage of bronchial asthma symptoms,reduce episodes of purpose.关键词：Bufei granules;asthma in remission stage;asthma control test;eosinophil;lung function;efficacy
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发布时间：2024-09-23 - 摘要：Objective: To observe the Bufei granules in the treatment of asthma in remission stage effect. Method: Fourty-three cases of asthma patients in remission, were randomly divided into a treatment group (22 cases), 21 cases of the control group. The treatment group given Bufei particles, the control group was given Bufei particle simulation agent. Treatment for 1 month, followed up for 3 months.Observation: asthma symptoms integral, asthma control test (ACT) score, indices of pulmonary function, blood eosinophil count index. Using IBM SPSS Statistics 19 statistical analysis. Result: Traditional Chinese Medicine(TCM) syndrome scoring, the treatment group decreased obviously, compared with the control group (P<0.01) has significant difference; patients with ACT score, the treatment group increased significantly, (P<0.01) has significant difference; and can reduce the peripheral blood eosinophil count, and before treatment is compared to P<0.05, have the difference of statistics; improved peak expiratory flow(PEF), with significant difference, (P<0.01); reduce attack frequency. Conclusion: Bufei granules can obviously improve the symptoms of patients with bronchial asthma in remission stage, and improve lung function, reduce the level of eosinophils(EOS), through the inhibition of airway inflammation, and to control the remission stage of bronchial asthma symptoms, reduce episodes of purpose.关键词：Bufei granules;asthma in remission stage;asthma control test;eosinophil;lung function;efficacy
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发布时间：2024-09-23 - 摘要：Objective:Preliminary analysis based on multiple linear regression model the poly fine Gouju particles intervene in the clinical efficacy and the mechanism of abnormal sperm,to explore further optimize the clinical treatment of the field Traditional Chinese Medicine.Method:Randomized control design,divided into standard control group of 65 cases;experimental group,65 cases were treated with poly pill poly fine Juju particles,first off,and two groups of sperm morphology,athletic ability and DNA fragmentation values indicators for detection analysis.Result: Multiple linear regression analysis of the experimental group before and after treatment,semen from each of the important indicators majority of Table 1-4 in each experimental group are statistically significant indicators of duplication,this complex statistical analysis on this study the clinical efficacy of the experimental group total efficiency of 42（64.61%）,control group,the total efficiency of 22（33.84%）.Conclusion:Poly fine Juju Gouju particles can effectively intervene in patients with Abnormal sperm.关键词：multivariate linear regression;Juju Gouju particles;abnormal sperm disease;morphology;movement ability;DNA fragmentation index
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发布时间：2024-09-23 - 摘要：Objective: Preliminary analysis based on multiple linear regression model the poly fine Gouju particles intervene in the clinical efficacy and the mechanism of abnormal sperm, to explore further optimize the clinical treatment of the field Traditional Chinese Medicine. Method: Randomized control design, divided into standard control group of 65 cases; experimental group, 65 cases were treated with poly pill poly fine Juju particles, first off, and two groups of sperm morphology, athletic ability and DNA fragmentation values indicators for detection analysis. Result: Multiple linear regression analysis of the experimental group before and after treatment, semen from each of the important indicators majority of Table 1-4 in each experimental group are statistically significant indicators of duplication, this complex statistical analysis on this study the clinical efficacy of the experimental group total efficiency of 42 (64.61%), control group, the total efficiency of 22 (33.84%). Conclusion: Poly fine Juju Gouju particles can effectively intervene in patients with Abnormal sperm.关键词：multivariate linear regression;Juju Gouju particles;abnormal sperm disease;morphology;movement ability;DNA fragmentation index
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发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Astragalus injection on urinary albumin and cytokines in patients with early diabetic nephropathy.To evaluate the efficacy and possible mechanisms in patients with early diabetic nephropathy.Method:Seventy-seven patients with early diabetic nephropathy were randomly divided into two groups: the control group（38 cases） was given Benazepril;Astragalus group（39 cases） was given Benazepril and Astragalus injection.These patients of two groups were treated for 30 days.Indicator of urinary microalbumin,β2-microglobulin（β2-MC）,urinary albumin excretion rate（UAER） and serum β2-MC,interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α）,vascular endothelial growth factor（VEGF）,transforming growth factor β1（TGF-β1） were observed before and after treatment.Result: Before the treatment,Indicator of urinary microalbumin,urinary albumin excretion rate,β2-microglobulin and serum β2-microglobulin,interleukin-6,tumor necrosis factor-α,vascular endothelial growth factor,transforming growth factor β1 were significantly higher than the health group（all P<0.01）,there were no significant difference in these indicators between two groups（all P>0.05）,it has comparability.After 30 days,there indicators had significantly improved compared with before treatment in patients of two groups（P<0.05 or P<0.01）,but patients in Astragalus group improved more significantly（P<0.05 or P<0.01）;Application Astragalus injection were no adverse reactions.Conclusion: It was safe and effective that Astragalus injection in the treatment of patients with early diabetic nephropathy,reduce the urinary mAlb,UAER,β2-MG and serum β2-MG significantly,inhibit the expression of IL-6,TNF-α,VEGF,TGF-β1 may be one of the mechanism of the protective effect of kidney.关键词：microalbumin;β2-microglobulin;urinary albumin excretion rate;interleukin-6;tumor necrosis factor-α;vascular endothelial growth factor;transforming growth factor β1
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发布时间：2024-09-23 - 摘要：Objective: To observe the effect of Astragalus injection on urinary albumin and cytokines in patients with early diabetic nephropathy. To evaluate the efficacy and possible mechanisms in patients with early diabetic nephropathy. Method: Seventy-seven patients with early diabetic nephropathy were randomly divided into two groups: the control group (38 cases) was given Benazepril;Astragalus group (39 cases) was given Benazepril and Astragalus injection. These patients of two groups were treated for 30 days. Indicator of urinary microalbumin,β2-microglobulin(β2-MC),urinary albumin excretion rate(UAER) and serum β2-MC,interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),vascular endothelial growth factor(VEGF),transforming growth factor β1(TGF-β1) were observed before and after treatment. Result: Before the treatment, Indicator of urinary microalbumin,urinary albumin excretion rate,β2-microglobulin and serum β2-microglobulin,interleukin-6,tumor necrosis factor-α,vascular endothelial growth factor,transforming growth factor β1 were significantly higher than the health group(all P<0.01),there were no significant difference in these indicators between two groups(all P>0.05), it has comparability.After 30 days, there indicators had significantly improved compared with before treatment in patients of two groups(P<0.05 or P<0.01),but patients in Astragalus group improved more significantly(P<0.05 or P<0.01);Application Astragalus injection were no adverse reactions. Conclusion: It was safe and effective that Astragalus injection in the treatment of patients with early diabetic nephropathy,reduce the urinary mAlb,UAER,β2-MG and serum β2-MG significantly,inhibit the expression of IL-6,TNF-α,VEGF,TGF-β1 may be one of the mechanism of the protective effect of kidney.关键词：microalbumin;β2-microglobulin;urinary albumin excretion rate;interleukin-6;tumor necrosis factor-α;vascular endothelial growth factor;transforming growth factor β1
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发布时间：2024-09-23 - 摘要：Objective:From the clinical trial to observe the therapeutic effects of Shenfu injection（SFI） on patients of chronic heart failure（CHF）.Method: Seventy-two CHF patients were randomly assigned to treatment group（36 cases） and control group（36 cases）.All were treated by the regular CHF treatment,but SFI was given to the patients in treatment group and Shengmai injection（SMI） to the patients in control group additionally.The course of treatment was two weeks.Before and after the treatments,the integral of Traditional Chinese Medicine（TCM） syndrome and quanlity of life（QOL）,6-minute walking distance,left ventricular ejection fraction（LVEF） and amino-terminal pro-brain natriuretic peptide（NT-proBNP） were observed.Result: Compared with before treatment,obvious improvement of clinical symptom and experiment index was obtained in two groups（P<0.05）.After treatment,the integral of TCM syndrome and QOL were reduced and 6-minute walking distance was increased further in treatment group（P<0.05）.The treatment group was superior to the control group in the amendment of the clinical symptom of gasp,fatigue,shortness of breath,cold extremities,and tongue picture（P<0.05）.There was no statistical difference in the increased LVEF and descended serum levels of NT-proBNP between the two groups.Conclusion: SFI showing definite effects on CHF with yang deficiency syndrome.Clinical using TCM injection still should follow the guidance of TCM syndrome differentiation theory.关键词：Shenfu Injection;chronic heart failure;amino-terminal pro-brain natriuretic peptide
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发布时间：2024-09-23 - 摘要：Objective: From the clinical trial to observe the therapeutic effects of Shenfu injection(SFI) on patients of chronic heart failure(CHF). Method: Seventy-two CHF patients were randomly assigned to treatment group(36 cases) and control group(36 cases). All were treated by the regular CHF treatment, but SFI was given to the patients in treatment group and Shengmai injection(SMI) to the patients in control group additionally. The course of treatment was two weeks. Before and after the treatments, the integral of Traditional Chinese Medicine(TCM) syndrome and quanlity of life(QOL), 6-minute walking distance, left ventricular ejection fraction(LVEF) and amino-terminal pro-brain natriuretic peptide(NT-proBNP) were observed. Result: Compared with before treatment, obvious improvement of clinical symptom and experiment index was obtained in two groups(P<0.05). After treatment, the integral of TCM syndrome and QOL were reduced and 6-minute walking distance was increased further in treatment group(P<0.05). The treatment group was superior to the control group in the amendment of the clinical symptom of gasp, fatigue, shortness of breath, cold extremities,and tongue picture(P<0.05). There was no statistical difference in the increased LVEF and descended serum levels of NT-proBNP between the two groups. Conclusion: SFI showing definite effects on CHF with yang deficiency syndrome. Clinical using TCM injection still should follow the guidance of TCM syndrome differentiation theory.关键词：Shenfu Injection;chronic heart failure;amino-terminal pro-brain natriuretic peptide
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发布时间：2024-09-23 - 摘要：Objective: To explore influence factors of female hyperplasia of mammary glands in Zhengzhou city. Method: In 2010 September-2011 May, one group of 435 outpatients with hyperplasia of the mammary glands in the second group of 242 women without hyperplasia of the mammary glands were collected.Using a case-control study method,with the information of the two groups which general, fertility, sociology etc. collection and statistics, to explore the influence factors of patients with hyperplasia of mammary glands by logistic regression analysis. Result: By multivariate logistic regression analysis, the risk factors are that dysmenorrhea, pregnancy times greater than 2 times, stream of people time is more than 2 times, moderate to low family income, interpersonal relations in general, profession mainly doing mental work,work to suppress with higher levels of anxiety and passive smoking;and the protective factors are that lean body mass, higher education, old age at menarche,birth order is more than 2 times,light diet and the occasional drink. Conclusion: The influencing factors of hyperplasia of mammary glands include menstrual and reproductive history, and occupation factors and emotional factors make a great impact on the disease.关键词：hyperplasia of mammary glands;influencing factor;case-control
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发布时间：2024-09-23 - 摘要：Objective:To explore influence factors of female hyperplasia of mammary glands in Zhengzhou city.Method: In 2010 September-2011 May,one group of 435 outpatients with hyperplasia of the mammary glands in the second group of 242 women without hyperplasia of the mammary glands were collected.Using a case-control study method,with the information of the two groups which general,fertility,sociology etc.collection and statistics,to explore the influence factors of patients with hyperplasia of mammary glands by logistic regression analysis.Result:By multivariate logistic regression analysis,the risk factors are that dysmenorrhea,pregnancy times greater than 2 times,stream of people time is more than 2 times,moderate to low family income,interpersonal relations in general,profession mainly doing mental work,work to suppress with higher levels of anxiety and passive smoking;and the protective factors are that lean body mass,higher education,old age at menarche,birth order is more than 2 times,light diet and the occasional drink.Conclusion:The influencing factors of hyperplasia of mammary glands include menstrual and reproductive history,and occupation factors and emotional factors make a great impact on the disease.关键词：hyperplasia of mammary glands;influencing factor;case-control
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发布时间：2024-09-23 - 摘要：Objective:Compare the proposed traditional prescription joint metalizing and mesalazine treatment the curative effect of ulcerative colitis,provide rational drug use advice for clinical treatment of ulcerative colitis.Method: Ninty cases of ulcerative colitis patients in our hospital from June 2010 to May 2011,randomly divided into treatment group（45 cases） and control group（45 cases）,were given the proposed traditional prescription joint mesalazine and mesalazine treatment respectively.After that,compare with two groups of treatment effective rate,under colonoscopy form change,C-reactive protein（CRP）,ergthrocyte sedimentration rate（ESR） etc,evaluate its effectiveness and safety.Result: The treatment effective rate and under colonoscopy form change of the two groups after treatment are all have statistical significance（P<0.05）.Conclusion: The curative effect of the proposed traditional prescription joint mesalazine is better than that of mesalazine.It is worthy of clinical application.
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发布时间：2024-09-23 - 摘要：Objective: Compare the proposed traditional prescription joint metalizing and mesalazine treatment the curative effect of ulcerative colitis, provide rational drug use advice for clinical treatment of ulcerative colitis. Method: Ninty cases of ulcerative colitis patients in our hospital from June 2010 to May 2011, randomly divided into treatment group (45 cases) and control group (45 cases), were given the proposed traditional prescription joint mesalazine and mesalazine treatment respectively. After that, compare with two groups of treatment effective rate, under colonoscopy form change,C-reactive protein(CRP),ergthrocyte sedimentration rate(ESR) etc, evaluate its effectiveness and safety. Result: The treatment effective rate and under colonoscopy form change of the two groups after treatment are all have statistical significance (P<0.05). Conclusion: The curative effect of the proposed traditional prescription joint mesalazine is better than that of mesalazine. It is worthy of clinical application.
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of tetramethylpyrazine on pulmonary artery hypertension in patients with chronic obstructive pulmonary disease at acute exacerbation. Method: Seventy-six patients with chronic obstructive pulmonary disease at acute exacerbation and pulmonary artery hypertension were randomly divided into two groups: the tetramethylpyrazine group and the control group (n=38 each). Basic routine therapy plus alprostadil was given to underlying diseases of both groups, Beside this basis, patients in the tetramethylpyrazine group were given tetramethylpyrazine injection 120 mg plus 5% glucose solution, intravenous infusion, once a day. Continuous medication lasted 14 days in both groups. Forty normal persons in outpatient were chosen as the health group. Blood gas analysis indicators, including partial pressure of oxygen in artery (PaO2) and blood oxygen saturation (SaO2); hemodynamic indicators, including left ventricular ejection fraction (LVEF), pulmonary artery systolic pressure (PASP), pulmonary artery diastolic pressure (PADP), mean pulmonary artery pressure (MPAP); biochemical indicators, including endothelin-1 (ET-1) and nitric oxide (NO) were detected in the health group and in both groups before and after treatment. Result: Before the treatment, there were significant differences in eight indicators (PaO2, SaO2, LVEF, PASP, PADP, MPAP, ET-1, NO) between the two groups and the health group (P<0.01), there were no significant difference in eight indicators of two groups. After 14 days, eight indicators had significantly improved compared with before treatment in patients of the two groups (P<0.05 or P<0.01), however patients in the tetramethylpyrazine group improved more significantly (P<0.05 or P<0.01). There were no adverse reactions associated with tetramethylpyrazine. Conclusion: It was safe and effective that tetramethylpyrazine for the treatment of patients with chronic obstructive pulmonary disease and pulmonary artery hypertension. The possible mechanism is likely related with inhibiting of the vasoconstrictor ET-1 and increasing in vasodilator factor NO to reduce pulmonary artery hypertension.关键词：chronic obstructive pulmonary disease;pulmonary artery hypertension;tetramethylpyrazine;endothelin-1;nitric oxide
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of tetramethylpyrazine on pulmonary artery hypertension in patients with chronic obstructive pulmonary disease at acute exacerbation.Method: Seventy-six patients with chronic obstructive pulmonary disease at acute exacerbation and pulmonary artery hypertension were randomly divided into two groups: the tetramethylpyrazine group and the control group（n=38 each）.Basic routine therapy plus alprostadil was given to underlying diseases of both groups,Beside this basis,patients in the tetramethylpyrazine group were given tetramethylpyrazine injection 120 mg plus 5% glucose solution,intravenous infusion,once a day.Continuous medication lasted 14 days in both groups.Forty normal persons in outpatient were chosen as the health group.Blood gas analysis indicators,including partial pressure of oxygen in artery（PaO2） and blood oxygen saturation（SaO2）;hemodynamic indicators,including left ventricular ejection fraction（LVEF）,pulmonary artery systolic pressure（PASP）,pulmonary artery diastolic pressure（PADP）,mean pulmonary artery pressure（MPAP）;biochemical indicators,including endothelin-1（ET-1） and nitric oxide（NO） were detected in the health group and in both groups before and after treatment.Result: Before the treatment,there were significant differences in eight indicators（PaO2,SaO2,LVEF,PASP,PADP,MPAP,ET-1,NO） between the two groups and the health group（P<0.01）,there were no significant difference in eight indicators of two groups.After 14 days,eight indicators had significantly improved compared with before treatment in patients of the two groups（P<0.05 or P<0.01）,however patients in the tetramethylpyrazine group improved more significantly（P<0.05 or P<0.01）.There were no adverse reactions associated with tetramethylpyrazine.Conclusion: It was safe and effective that tetramethylpyrazine for the treatment of patients with chronic obstructive pulmonary disease and pulmonary artery hypertension.The possible mechanism is likely related with inhibiting of the vasoconstrictor ET-1 and increasing in vasodilator factor NO to reduce pulmonary artery hypertension.关键词：chronic obstructive pulmonary disease;pulmonary artery hypertension;tetramethylpyrazine;endothelin-1;nitric oxide
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发布时间：2024-09-23 - 摘要：Objective: To investigate the efficacy of ganglioside supplemented by Danshen injection in the treatment of neonatal hypoxic-ischemic encephalopathy (HIE). Method: Seventy neonates with HIE were randomly divided into observation group and the control group, the control group were given symptomatic and supportive treatment, the observation group was received ganglioside and Danshen injection. The efficacy, neonatal behavioral neurological assessment(NBNA) scores changes and the improvement of convulsions, disturbance of consciousness, abnormal primitive reflexes and dystonia were observed and compared. Result: The effective rate of the observation group was 94.29%, significantly higher than that of the control group (77.14%), the difference had statistically significant (P<0.05). After one week and two weeks of treatment, the NBNA scores of two groups were significantly improved compared with before treatment, there were statistically significant (P<0.05). Compared with the control group, NBNA scores in the observation group after treatment were improved significantly (P<0.05). The time of convulsions, disturbance of consciousness, the original abnormal reflexes and dystonia were improved in the observation group significantly compared with those of the control group (P<0.05). Conclusion: Ganglioside supplemented by Danshen injection is effective in the treatment of HIE, and shows marked clinical value.关键词：ganglioside;Danshen injection;neonatal hypoxic-ischemic encephalopathy
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发布时间：2024-09-23 - 摘要：Objective:To investigate the efficacy of ganglioside supplemented by Danshen injection in the treatment of neonatal hypoxic-ischemic encephalopathy（HIE）.Method:Seventy neonates with HIE were randomly divided into observation group and the control group,the control group were given symptomatic and supportive treatment,the observation group was received ganglioside and Danshen injection.The efficacy,neonatal behavioral neurological assessment（NBNA） scores changes and the improvement of convulsions,disturbance of consciousness,abnormal primitive reflexes and dystonia were observed and compared.Result:The effective rate of the observation group was 94.29%,significantly higher than that of the control group（77.14%）,the difference had statistically significant（P<0.05）.After one week and two weeks of treatment,the NBNA scores of two groups were significantly improved compared with before treatment,there were statistically significant（P<0.05）.Compared with the control group,NBNA scores in the observation group after treatment were improved significantly（P<0.05）.The time of convulsions,disturbance of consciousness,the original abnormal reflexes and dystonia were improved in the observation group significantly compared with those of the control group（P<0.05）.Conclusion:Ganglioside supplemented by Danshen injection is effective in the treatment of HIE,and shows marked clinical value.关键词：ganglioside;Danshen injection;neonatal hypoxic-ischemic encephalopathy
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发布时间：2024-09-23 - 摘要：Objective: To observe the clinical efficacy of combination of traditional Chinese medicine and western medicine for treating acute respiratory distress syndrome (ARDS) after chest trauma. Method: Eighty patients with ARDS treated in our hospital divided into 2 groups randomly, the control group (routine western medicine therapy) and the treatment group (modified Liangge San one dose daily, 10 d for a course, based on the routine western medicine). Hospitalization duration, mechanical ventilation time, oxygenation index, the change of arterial partial pressure of oxygen, complications and death rate in the 2 groups were observed and compared. Result: Arterial partial pressure of oxygen at 48, 72 h and oxygenation index at 24.36, 48, 72 h after treatment in the treatment group improved more evidently than those in the control group (P<0.05). Comparisons on mechanical ventilation time [(7.31±3.12)d vs (10.22±4.42)d], average hospitalization days [(12.32±2.35)d vs (16.43±3.43)d] and death rate (22.5% vs 42.5%) between treatment group and control group showed significant differences(P<0.05). Conclusion: Combined treatment of traditional Chinese medicine and western medicine for treat ARDS after chest trauma can not only reduce evidently mechanical ventilation time and hospitalization duration, but also improve success rate of rescue and reduce mortality.关键词：combined treatment of traditional Chinese and western medicine;chest trauma;acute respiratory distress syndrome
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发布时间：2024-09-23 - 摘要：Objective:To observe the clinical efficacy of combination of traditional Chinese medicine and western medicine for treating acute respiratory distress syndrome（ARDS） after chest trauma.Method: Eighty patients with ARDS treated in our hospital divided into 2 groups randomly,the control group（routine western medicine therapy） and the treatment group（modified Liangge San one dose daily,10 d for a course,based on the routine western medicine）.Hospitalization duration,mechanical ventilation time,oxygenation index,the change of arterial partial pressure of oxygen,complications and death rate in the 2 groups were observed and compared.Result: Arterial partial pressure of oxygen at 48,72 h and oxygenation index at 24.36,48,72 h after treatment in the treatment group improved more evidently than those in the control group（P<0.05）.Comparisons on mechanical ventilation time[（7.31±3.12）d vs（10.22±4.42）d],average hospitalization days [（12.32±2.35）d vs（16.43±3.43）d] and death rate（22.5% vs 42.5%） between treatment group and control group showed significant differences（P<0.05）.Conclusion: Combined treatment of traditional Chinese medicine and western medicine for treat ARDS after chest trauma can not only reduce evidently mechanical ventilation time and hospitalization duration,but also improve success rate of rescue and reduce mortality.关键词：combined treatment of traditional Chinese and western medicine;chest trauma;acute respiratory distress syndrome
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发布时间：2024-09-23 - 摘要：Objective:To study the influence of Danhong injection on cerebrovascular hemodynamic parameters and nerve function related factors in patients with transient ischemic attack.Method:Fifty-eight patients with transient ischemic attack in our hospital were selected and randomly divided into control group and observation group with 29 cases in each group,the control group were treated with routine treatment,the observation group were treated with Danhong injection 40 mL+5% glucose solution iv,daily,for 2 weeks,based on the treatment of control group.Then cerebrovascular hemodynamic parameters and serum neuron specific enolase（NSE）,S100B protein（S-100B）,myelin basic protein（MBP）,glial fibrillary acidic protein（GFAP） of two groups before and after the treatment after 1 and 2 weeks were compared.Result:The mean flow velocity（Vmean）,maximum-portal flow velocity（Vmax）,minimum flow velocity（Vmin） and mean blood flow（Qmean） of observation group after the treatment for 1 and 2 weeks were higher than those in control group,resistance（R） were lower than that of control group,serum NSE,S-100B,MBP and GFAP were all lower than those of control group（all P<0.05）.Conclusion:The influence of Danhong injection on cerebrovascular hemodynamic parameters and nerve function related factors in patients with transient ischemic attack is significant,and the injection can effectively improve the blood supply in brain.关键词：Danhong injection;transient ischemic attack;cerebrovascular hemodynamic parameters;nerve function related factors
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发布时间：2024-09-23 - 摘要：Objective: To study the influence of Danhong injection on cerebrovascular hemodynamic parameters and nerve function related factors in patients with transient ischemic attack. Method: Fifty-eight patients with transient ischemic attack in our hospital were selected and randomly divided into control group and observation group with 29 cases in each group,the control group were treated with routine treatment,the observation group were treated with Danhong injection 40 mL+5% glucose solution iv,daily,for 2 weeks, based on the treatment of control group. Then cerebrovascular hemodynamic parameters and serum neuron specific enolase(NSE),S100B protein (S-100B),myelin basic protein (MBP),glial fibrillary acidic protein (GFAP) of two groups before and after the treatment after 1 and 2 weeks were compared. Result: The mean flow velocity(Vmean),maximum-portal flow velocity(Vmax),minimum flow velocity (Vmin) and mean blood flow(Qmean) of observation group after the treatment for 1 and 2 weeks were higher than those in control group,resistance (R) were lower than that of control group,serum NSE,S-100B,MBP and GFAP were all lower than those of control group (all P<0.05). Conclusion: The influence of Danhong injection on cerebrovascular hemodynamic parameters and nerve function related factors in patients with transient ischemic attack is significant,and the injection can effectively improve the blood supply in brain.关键词：Danhong injection;transient ischemic attack;cerebrovascular hemodynamic parameters;nerve function related factors
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of Naomaitai capsule on cognitive dysfunction of vascular dementia patients.Method: Eighty four vascular dementia patients were randomly divided into therapeutic group and control group（n=41 each）.The therapeutic group was treated by Naomaitai capsule（2 capsules,tid） and the control group by Naofukang（2 tablets,tid）.The therapeutic course was 12 weeks.Before and after treatment,mimi-mental state examination（MMSE） grade and variation of frenquently appeared potential（P300） were evaluated.Clinical efficacy was evaluated for the two groups.Result: After the therapeutic course,the total efficacy of the observation group was better than that of the control group,the difference was statistically significant（p<0.05）.In two groups MMSE scores,P300 latency and P300 amplitude was significantly improved after treatment compared with those before treatment（P<0.05 or P<0.01）.The improvement in the treatment group was better than that in the controls.Conclusion: Naomaitai capsule for treating vascular dementia shows good efficacy and less adverse reactions.The capsule can promote cognitive recovery.关键词：Namaitai capsule;vascular dementia;MMSE scores;P300;safety
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of Naomaitai capsule on cognitive dysfunction of vascular dementia patients. Method: Eighty four vascular dementia patients were randomly divided into therapeutic group and control group (n=41 each). The therapeutic group was treated by Naomaitai capsule (2 capsules, tid) and the control group by Naofukang (2 tablets, tid). The therapeutic course was 12 weeks. Before and after treatment, mimi-mental state examination (MMSE) grade and variation of frenquently appeared potential (P300) were evaluated. Clinical efficacy was evaluated for the two groups. Result: After the therapeutic course, the total efficacy of the observation group was better than that of the control group, the difference was statistically significant (p<0.05). In two groups MMSE scores, P300 latency and P300 amplitude was significantly improved after treatment compared with those before treatment (P<0.05 or P<0.01). The improvement in the treatment group was better than that in the controls. Conclusion: Naomaitai capsule for treating vascular dementia shows good efficacy and less adverse reactions. The capsule can promote cognitive recovery.关键词：Namaitai capsule;vascular dementia;MMSE scores;P300;safety
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发布时间：2024-09-23 - 摘要：Objective:To explore the Clinical treatment of adhesive ileus by Dachengqi Tang.Method: We analyzed 113 cases suffered from adhesive ileus.The 113 cases were randomly divided into 2 groups.Fifty-eight cases in treatment group had been given conventional therapy and Dachengqi Tang,and the other 55 cases in control group had been given conventional therapy.The total effective,exhaust time,defecation time,duration of stomach tube application,flow volume led by stomach tube,hospitalized duration and adverse reactions were compared between the two groups.Result: The total effective were 96.5% in the treatment group,and 76.4% in control group（P<0.05）.There were significant differences in the exhaust time（73.2±3.2）,（123.7±2.9） h,defecation time（99.6±3.2）,（143.2±2.0） h,duration of stomach tube application（104.3±3.3）,（163.5±2.1） h,flow volume led by stomach tube（380.2±0.5）,（640.6±0.9） mL,hospitalized duration（8.1±0.6）,（12.3±0.8） d,the incidence of adverse reaction（1.7%,10.9%） between the treatment group and the control group（P<0.05）.Concolusion: Combine treatment of traditional Chinese with western medicine for adhesion ileus shows better curative effect compared with that of pure western medicine treatment group.It is safe and promising clinically.关键词：adhesive ileus;Dachengqi Tang;conventional therapy by western medicine
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发布时间：2024-09-23 - 摘要：Objective: To explore the Clinical treatment of adhesive ileus by Dachengqi Tang. Method: We analyzed 113 cases suffered from adhesive ileus. The 113 cases were randomly divided into 2 groups. Fifty-eight cases in treatment group had been given conventional therapy and Dachengqi Tang, and the other 55 cases in control group had been given conventional therapy. The total effective, exhaust time, defecation time, duration of stomach tube application, flow volume led by stomach tube, hospitalized duration and adverse reactions were compared between the two groups. Result: The total effective were 96.5% in the treatment group,and 76.4% in control group (P<0.05). There were significant differences in the exhaust time (73.2±3.2), (123.7±2.9) h, defecation time (99.6±3.2), (143.2±2.0) h, duration of stomach tube application (104.3±3.3), (163.5±2.1) h, flow volume led by stomach tube (380.2±0.5), (640.6±0.9) mL, hospitalized duration (8.1±0.6), (12.3±0.8) d, the incidence of adverse reaction (1.7%, 10.9%) between the treatment group and the control group(P<0.05). Concolusion: Combine treatment of traditional Chinese with western medicine for adhesion ileus shows better curative effect compared with that of pure western medicine treatment group. It is safe and promising clinically.关键词：adhesive ileus;Dachengqi Tang;conventional therapy by western medicine
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发布时间：2024-09-23 - 摘要：Objective:Search all studies that have been published in the world with regard to the curative effect of traditional Chinese medicine on essential hypertension and analyze them by means of meta-analysis,in order to give some evidences for the doctors treating hypertension.Method: Evaluate strictly all the studies that have been searched and do meta-analysis in the eligible ones by the software RevMan5.1.Evaluate the curative effect of Traditional Chinese medicine on essential hypertension.Result: Totally 19 studies including 1 635 cases were analyzed.Comparison between（traditional Chinese medicine or traditional Chinese medicine plus western medicine） and western medicine in antihypertensive effectP<0.000 01],comparison between Traditional Chinese medicine and western medicine in antihypertensive effectP<0.000 01],Comparison between（traditional Chinese medicine or traditional Chinese medicine plus western medicine） and western medicine in curative effect of symptoms P=0.44],comparison between traditional Chinese medicine and western medicine in curative effect of symptomsP < 0.000 01].Conclusion: Traditional Chinese medicine play a role in antihypertensive effect and improve symptoms.关键词：hypertension;traditional Chinese medicine;Meta-analysis
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发布时间：2024-09-23 - 摘要：Objective: Search all studies that have been published in the world with regard to the curative effect of traditional Chinese medicine on essential hypertension and analyze them by means of meta-analysis, in order to give some evidences for the doctors treating hypertension. Method: Evaluate strictly all the studies that have been searched and do meta-analysis in the eligible ones by the software RevMan5.1.Evaluate the curative effect of Traditional Chinese medicine on essential hypertension. Result: Totally 19 studies including 1 635 cases were analyzed. Comparison between (traditional Chinese medicine or traditional Chinese medicine plus western medicine) and western medicine in antihypertensive effect [OR=2.05,95%CI( 1.56,2.69),Z=5.15,P<0.000 01], comparison between Traditional Chinese medicine and western medicine in antihypertensive effect [OR=2.05,95%CI(1.56,2.69),Z=5.15,P<0.000 01], Comparison between (traditional Chinese medicine or traditional Chinese medicine plus western medicine) and western medicine in curative effect of symptoms [OR=1.17,95%CI( 0.79,1.73),Z=0.78,P=0.44], comparison between traditional Chinese medicine and western medicine in curative effect of symptoms [OR=5.76,95%CI( 3.68,9.03),Z=7.65,P < 0.000 01]. Conclusion: Traditional Chinese medicine play a role in antihypertensive effect and improve symptoms.关键词：hypertension;traditional Chinese medicine;Meta-analysis
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发布时间：2024-09-23 - 摘要：Objective:To conduct a review to explore the efficacy of the Shengmai injection in treating ischemic stroke.Method:Search all randomized controlled trails（RCTs） that can be found with regard to the effectiveness of the Shengmai injection for ischemic stroke.Evaluate strictly all the studies that have been searched and adopt the studies that can be analyzed by means of Meta-analysis.Calculate the relative risk（RR） for effective rate of the Shengmai injection for ischemic stroke.Result: Totally 12 RCTs including 960 cases were analyzed.The cumulative cases in using the Shengmai Injection for Ischemic stroke group were 493,in which 444 cases were effective,while in the group treat without shengmai injection was 467 and 351 respectively.The pooled RR was 1.20,with a 95%confidence interval（1.13,1.27）.Conclusion: This review showed that the Shengmai injection is effective for ischemic stroke.关键词：Shengmai Injection;ischemic stroke;Meta-analysis
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发布时间：2024-09-23 - 摘要：Objective: To conduct a review to explore the efficacy of the Shengmai injection in treating ischemic stroke. Method: Search all randomized controlled trails (RCTs) that can be found with regard to the effectiveness of the Shengmai injection for ischemic stroke. Evaluate strictly all the studies that have been searched and adopt the studies that can be analyzed by means of Meta-analysis. Calculate the relative risk (RR) for effective rate of the Shengmai injection for ischemic stroke. Result: Totally 12 RCTs including 960 cases were analyzed. The cumulative cases in using the Shengmai Injection for Ischemic stroke group were 493,in which 444 cases were effective,while in the group treat without shengmai injection was 467 and 351 respectively. The pooled RR was 1.20, with a 95%confidence interval (1.13, 1.27). Conclusion: This review showed that the Shengmai injection is effective for ischemic stroke.关键词：Shengmai Injection;ischemic stroke;Meta-analysis
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发布时间：2024-09-23 - 摘要：Objective:Eighteen incompatible herbs and Nineteen herbs of mutual antagonism,the incompatibility between the traditional Chinese medicine（TCM） has had the history of thousands of years so far.Whether Eighteen incompatible herbs and Nineteen herbs of mutual antagonism are incompatibility or not has become one of the hot spot of academia right now.Method:The medicine catalogue for the national basic medical insurance,injury insurance and maternity insurance was issued by the department of social security and Human Resource,which improving the level of clinical use of drugs.Result:According to statistics,there are 987 kinds of patent Chinese medicine products（CPMs） in medicine catalogue for the national basic medical insurance,injury insurance and maternity insurance（2009 Edition）,in which 5 CPMs contain eighteen incompatible herbs and 4 CPMs contain Nineteen herbs of mutual antagonism.Conclusion:This paper analyze CPMs containing Eighteen incompatible herbs and Nineteen herbs of mutual antagonism to offer some elicitation for promoting rational drug use in the clinic.关键词：medical insurance list;Chinese patent medicine;Eighteen incompatible herbs;nineteen herbs of mutual antagonism
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发布时间：2024-09-23 - 摘要：Objective: Eighteen incompatible herbs and Nineteen herbs of mutual antagonism, the incompatibility between the traditional Chinese medicine(TCM) has had the history of thousands of years so far. Whether Eighteen incompatible herbs and Nineteen herbs of mutual antagonism are incompatibility or not has become one of the hot spot of academia right now. Method: The medicine catalogue for the national basic medical insurance, injury insurance and maternity insurance was issued by the department of social security and Human Resource, which improving the level of clinical use of drugs. Result: According to statistics, there are 987 kinds of patent Chinese medicine products (CPMs) in medicine catalogue for the national basic medical insurance, injury insurance and maternity insurance (2009 Edition), in which 5 CPMs contain eighteen incompatible herbs and 4 CPMs contain Nineteen herbs of mutual antagonism. Conclusion: This paper analyze CPMs containing Eighteen incompatible herbs and Nineteen herbs of mutual antagonism to offer some elicitation for promoting rational drug use in the clinic.关键词：medical insurance list;Chinese patent medicine;Eighteen incompatible herbs;nineteen herbs of mutual antagonism
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发布时间：2024-09-23 - 摘要：To discuss status and proposals of applying proteomics to research essence of liver injury. Looking through the related literatures from home and abroad, then analyzing and compiling them.①Proteomics technique has been applied to many types of liver injury induced by drug, carbon tetrachloride (CCl4), alcohol, lipopolysaccharides (LPS), immune related, thioacetamide, radiation, bromobenzene, hydrazine, or non-alcoholic fatty liver disease (NAFLD). ②The protein samples for analysis are mainly taken from the animal liver, serum, urine, bile acids, mitochondria, etc. ③Main analyzing tools of proteomics are two-dimensional gel electrophoresis (2-DE), Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and multi-stage MS. ④Research on proteome mechanism of liver injury needs to be strengthened. ⑤Hepatoprotective effect of drugs is not enough through proteomics. We should strengthen researches on essence of liver injury and essence-based hepatoprotection of drugs through proteomics.关键词：proteomics;liver injury;drug-induce liver injury;hepatoprotective effect;two-dimensional gel electrophoresis;mass spectrometry;essence
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发布时间：2024-09-23 - 摘要：To discuss status and proposals of applying proteomics to research essence of liver injury.Looking through the related literatures from home and abroad,then analyzing and compiling them.①Proteomics technique has been applied to many types of liver injury induced by drug,carbon tetrachloride（CCl4）,alcohol,lipopolysaccharides（LPS）,immune related,thioacetamide,radiation,bromobenzene,hydrazine,or non-alcoholic fatty liver disease（NAFLD）.②The protein samples for analysis are mainly taken from the animal liver,serum,urine,bile acids,mitochondria,etc.③Main analyzing tools of proteomics are two-dimensional gel electrophoresis（2-DE）,Matrix-assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF-MS） and multi-stage MS.④Research on proteome mechanism of liver injury needs to be strengthened.⑤Hepatoprotective effect of drugs is not enough through proteomics.We should strengthen researches on essence of liver injury and essence-based hepatoprotection of drugs through proteomics.关键词：proteomics;liver injury;drug-induce liver injury;hepatoprotective effect;two-dimensional gel electrophoresis;mass spectrometry;essence
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发布时间：2024-09-23 - 摘要：To investigate difference between Chinese pharmacopeia commission(ChP) and United States Pharmacopeia commission(USP) at enacting and revising plant medicines standards,and suggest reasonable proposals to revision of standards for Chinese medicine in China.By analyze the current situation of standards revision in USA and China,basic research of standards in "Chinese Pharmacopeia" should be improved;Focus on coordination and harmonization of series of varieties and wholeness of analysis methods;Difficulty of analysis consistency and chromatography normalization could be solved by applying control extract of botanicals or the control preparation;Pay high attention to the overall safety of drugs and choice of detection methods;We recommended to learn from USP,such as experience of drafting revised standards with low-cost,management model and philosophy of varieties of responsible person system.Found out insufficient in ChP by analyzing difference between ChP and USP,these advanced experience in USP should be mirrored and shifted to ChP,in order to provide a reference for development of our botanical drugs.关键词：United States Pharmacopeia;Chinese Pharmacopeia;enacting and revising plant medicines standards;reference
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发布时间：2024-09-23 - 摘要：To investigate difference between Chinese pharmacopeia commission（ChP） and United States Pharmacopeia commission（USP） at enacting and revising plant medicines standards,and suggest reasonable proposals to revision of standards for Chinese medicine in China.By analyze the current situation of standards revision in USA and China,basic research of standards in "Chinese Pharmacopeia" should be improved;Focus on coordination and harmonization of series of varieties and wholeness of analysis methods;Difficulty of analysis consistency and chromatography normalization could be solved by applying control extract of botanicals or the control preparation;Pay high attention to the overall safety of drugs and choice of detection methods;We recommended to learn from USP,such as experience of drafting revised standards with low-cost,management model and philosophy of varieties of responsible person system.Found out insufficient in ChP by analyzing difference between ChP and USP,these advanced experience in USP should be mirrored and shifted to ChP,in order to provide a reference for development of our botanical drugs.关键词：United States Pharmacopeia;Chinese Pharmacopeia;enacting and revising plant medicines standards;reference
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发布时间：2024-09-23 - 摘要：<正>网址:www.syfjxzz.com《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期
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发布时间：2024-09-23
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