最新刊期
卷 18 ， 期 18 ， 2012
- 摘要：Objective: To analyze the composition principles of the external use prescriptions for urticaria collected in 〈The Prescription Dictionary of Traditional Chinese Medicine〉. Method: The external use formulas used for urticaria in 〈The Prescription Dictionary of Traditional Chinese Medicine〉 were collected, then entered the Traditional Chinese Medicine Inheritance Support System (TCMISS;V1.1). By using data mining methods, composition principles of the prescriptions were analyzed. Result: Based on the analysis of 134 cases of external use formulas, the frequency of each herb included in the database was computed, twenty-seven core combinations and 5 new prescriptions were mined from the database. Conclusion: The composition principles of the formulas for urticaria collected in 〈The Prescription Dictionary of Traditional Chinese Medicine〉 were summarized. This would be the foundation for further research and development of new drugs for treating urticaria.
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发布时间：2024-09-23 - 摘要：Objective:To analyze the composition principles of the external use prescriptions for urticaria collected in 〈The Prescription Dictionary of Traditional Chinese Medicine〉.Method:The external use formulas used for urticaria in 〈The Prescription Dictionary of Traditional Chinese Medicine〉 were collected,then entered the Traditional Chinese Medicine Inheritance Support System（TCMISS;V1.1）.By using data mining methods,composition principles of the prescriptions were analyzed.Result:Based on the analysis of 134 cases of external use formulas,the frequency of each herb included in the database was computed,twenty-seven core combinations and 5 new prescriptions were mined from the database.Conclusion:The composition principles of the formulas for urticaria collected in 〈The Prescription Dictionary of Traditional Chinese Medicine〉 were summarized.This would be the foundation for further research and development of new drugs for treating urticaria.
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发布时间：2024-09-23 - 摘要：Objective: To optimize purification technology of total saponins, flavonoids and lignins from K-CoxB-JN by macroporous resin. Method: Taking the contents of total saponins, flavonoids and lignins as indexes,adsorption and desorption properties of 10 kinds macroporous resins were compared,based on these,maximum sample volume,sample flow rate and elution solvent were investigated by single-factor test. Result: D101 macroporous resin gave good static-dynamic and adsorption-desorption characteristics for all components,optimized purification technology was:crude drug concentration of alcohol extraction solution 0.5 g ·mL-1,herbs-wet resin 1: 4,adsorption flow rate 2 BV ·h-1, washed with 3 BV distilled water,4 BV of 70% ethanol as elution solvent.Under these conditions,purity of total saponins, total flavonoids and total lignins were enriched from 5.36%,0.3%,2.26% to 53.82%,3.01%,22.87%. Conclusion: This optimum purification technology of K-CoxB-JN was simple, stable and effective with good purification effect,it could be promoted in large-scale production.关键词：K-CoxB-JN;total saponins;total flavonoids;total lignins;Macroporous resin;purification
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发布时间：2024-09-23 - 摘要：Objective:To optimize purification technology of total saponins,flavonoids and lignins from K-CoxB-JN by macroporous resin.Method:Taking the contents of total saponins,flavonoids and lignins as indexes,adsorption and desorption properties of 10 kinds macroporous resins were compared,based on these,maximum sample volume,sample flow rate and elution solvent were investigated by single-factor test.Result:D101 macroporous resin gave good static-dynamic and adsorption-desorption characteristics for all components,optimized purification technology was:crude drug concentration of alcohol extraction solution 0.5 g·mL-1,herbs-wet resin 1 ∶ 4,adsorption flow rate 2 BV·h-1,washed with 3 BV distilled water,4 BV of 70% ethanol as elution solvent.Under these conditions,purity of total saponins,total flavonoids and total lignins were enriched from 5.36%,0.3%,2.26% to 53.82%,3.01%,22.87%.Conclusion:This optimum purification technology of K-CoxB-JN was simple,stable and effective with good purification effect,it could be promoted in large-scale production.关键词：K-CoxB-JN;total saponins;total flavonoids;total lignins;Macroporous resin;purification
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction and purification technology of total flavones from Aster ageratoides. Method: With the content of total flavones as index,extraction technology conditions of total flavones was optimized by orthogonal test;Type of macroporous resin was selected with static adsorption capacity and desorption rate as indexes,and to optimized purification technology of total flavones. Result: Optimum extraction conditions were as follows:soaking extracted 3 times with 10 times the amount of 60% ethanol at 50℃. HPD-500 type macro porous resin showed better adsorption and desorption property,optimum purification conditions were as follows:sample solution was prepared with the concentration of 50 g · L-1, subjected to HPD-500 type macroporous resin column chromatography with load ratio of 16 mg total flavones per gram of resin,standing for 1 hour, column was eluted with 4 BV water before being eluted with 2 BV 70% ethanol.Under these conditions,purity of total flavones was 76.7%,which enhanced 3.83 times. Conclusion: This optimized technology was convenient and practical,it could provide a reference for industrial production of A. ageratoides.关键词：Aster ageratoides;total flavones;extraction;purification;technology optimization
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction and purification technology of total flavones from Aster ageratoides.Method:With the content of total flavones as index,extraction technology conditions of total flavones was optimized by orthogonal test;Type of macroporous resin was selected with static adsorption capacity and desorption rate as indexes,and to optimized purification technology of total flavones.Result:Optimum extraction conditions were as follows:soaking extracted 3 times with 10 times the amount of 60% ethanol at 50 ℃.HPD-500 type macro porous resin showed better adsorption and desorption property,optimum purification conditions were as follows:sample solution was prepared with the concentration of 50 g · L-1,subjected to HPD-500 type macroporous resin column chromatography with load ratio of 16 mg total flavones per gram of resin,standing for 1 hour,column was eluted with 4 BV water before being eluted with 2 BV 70% ethanol.Under these conditions,purity of total flavones was 76.7%,which enhanced 3.83 times.Conclusion:This optimized technology was convenient and practical,it could provide a reference for industrial production of A.ageratoides.关键词：Aster ageratoides;total flavones;extraction;purification;technology optimization
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发布时间：2024-09-23 - 摘要：Objective: To compare the contents of epiberberine,coptisine,berberine and palmatine in different processed of Coptis chinensis. Method: Quantitative analysis multi-components by single marker was used to determine the the contents of four alkaloids in different processed C. chinensis.Chromatographic conditions were:Agilent TC-C18 column(2) (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.05 mol · L-1 potassium dihydrogen phosphate(50: 50,plus 0.4 g sodium dodecyl sulfate per 100 mL,phosphate-regulating pH 4.0),detection wavelength 345 nm,column temperature 25℃,flow rate 1 mL · min-1,number of theoretical plates calculated to berberine hydrochloride should be greater than 2 000. Result: The contents of epiberberine, coptisine,berberine and palmatine in different processed of C. chinensis had different degrees of reduction. Conclusion: Established determination method in this test was rapid and economical,it could be used as quality testing method for different processed of C. chinensis.关键词：quantitative analysis multi-components by single marker;Coptis chinensis;processed product;alkaloid
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发布时间：2024-09-23 - 摘要：Objective:To compare the contents of epiberberine,coptisine,berberine and palmatine in different processed of Coptis chinensis.Method:Quantitative analysis multi-components by single marker was used to determine the the contents of four alkaloids in different processed C.chinensis.Chromatographic conditions were:Agilent TC-C18 column（2）（4.6 mm×250 mm,5 μm）,mobile phase of acetonitrile-0.05 mol · L-1 potassium dihydrogen phosphate（50 ∶ 50,plus 0.4 g sodium dodecyl sulfate per 100 mL,phosphate-regulating pH 4.0）,detection wavelength 345 nm,column temperature 25 ℃,flow rate 1 mL · min-1,number of theoretical plates calculated to berberine hydrochloride should be greater than 2 000.Result:The contents of epiberberine,coptisine,berberine and palmatine in different processed of C.chinensis had different degrees of reduction.Conclusion:Established determination method in this test was rapid and economical,it could be used as quality testing method for different processed of C.chinensis.关键词：quantitative analysis multi-components by single marker;Coptis chinensis;processed product;alkaloid
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- 410
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发布时间：2024-09-23 - 摘要：Objective: To investigate preparation process of low molecular weight glycopeptide from Panax ginseng by dialysis method, and study on its physical and chemical properties, purity and molecular weight distribution. Method: With physical and chemical properties and yield as indexes,preparation technology of low molecular weight glycopeptide from P. ginseng by dialysis was investigated by single-factor test,purity and molecular weight distribution were determined by HPLC and GPC software. Result: Preparation process was:closure molecular weight of dialysis bag 1 000 Da, added 6 L water and dialysis time 4 h.The content of total glycopeptide from P. ginseng was 92.49%, yield was 18.95%,it was a mixture with molecular weight 300-3 500 Da. Conclusion: This preparation technology of dialysis was simple,the content of total glycopeptide was high, and molecular weight was low.关键词：Panax ginseng;glycopeptides;dialysis;physical and chemical properties;HPLC
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发布时间：2024-09-23 - 摘要：Objective:To investigate preparation process of low molecular weight glycopeptide from Panax ginseng by dialysis method,and study on its physical and chemical properties,purity and molecular weight distribution.Method:With physical and chemical properties and yield as indexes,preparation technology of low molecular weight glycopeptide from P.ginseng by dialysis was investigated by single-factor test,purity and molecular weight distribution were determined by HPLC and GPC software.Result:Preparation process was:closure molecular weight of dialysis bag 1 000 Da,added 6 L water and dialysis time 4 h.The content of total glycopeptide from P.ginseng was 92.49%,yield was 18.95%,it was a mixture with molecular weight 300-3 500 Da.Conclusion:This preparation technology of dialysis was simple,the content of total glycopeptide was high,and molecular weight was low.关键词：Panax ginseng;glycopeptides;dialysis;physical and chemical properties;HPLC
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction technology of Pueraria lobata. Method: With the content of puerarin and pharmacodynamics of in vitro myocardial cell viability as comprehensive analysis indexes,orthogonal test was used to optimize extraction technology with extraction time,solid-liquid ratio,extraction times and the concentration of ethanol as factors. Result: The best extract condition of P. lobata was:refluxing extracted 2 times with 6 times the amount of 70% ethanol,1.5 h per time. Conclusion: Combining with chemical and pharmacodynamic indexes to optimize extraction and purification process could better guarantee quality of preparations,and this optimized technology was stable and feasible.关键词：Pueraria lobata;puerarin;orthogonal test;drug-containing plasma;pharmacodynamic
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of Pueraria lobata.Method:With the content of puerarin and pharmacodynamics of in vitro myocardial cell viability as comprehensive analysis indexes,orthogonal test was used to optimize extraction technology with extraction time,solid-liquid ratio,extraction times and the concentration of ethanol as factors.Result:The best extract condition of P.lobata was:refluxing extracted 2 times with 6 times the amount of 70% ethanol,1.5 h per time.Conclusion:Combining with chemical and pharmacodynamic indexes to optimize extraction and purification process could better guarantee quality of preparations,and this optimized technology was stable and feasible.关键词：Pueraria lobata;puerarin;orthogonal test;drug-containing plasma;pharmacodynamic
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the feasibility of removing heavy metal ions from the extract of Chinese herbal medicines containing alkaloids by γ-mercaptopropyl-modified silica gel（MPS）.Method:Some influence factors on removal of cadmium ion in the aqueous extract of Coptidis Rhizoma were studied,such as the amount of MPS,flow velocity,ratio of column diameter to height and temperature.The chemical changes were evaluated by the HPLC fingerprint profiles and dried extract content,before and after the removal of cadmium ion.Result:The optimum parameters were as follows:the ratio of medicinal herb to MPS 40∶1,1∶3 of ratio of diameter to height,10 BV · h-1 flow velocity,and the room temperature.The removing rate of cadmium ion and copper ion reached 81.3% and 35.9%,respectively.The content of magnesium and zinc ion hardly changed.Dried extract content of aqueous solution of Coptidis Rhizoma decreased by 1.3%,and the similarity of HPLC fingerprint profiles was 0.99.Conclusion:MPS can remove efficiently heavy metal ions and there was no significant change on the chemical components of aqueous solution of Coptidis Rhizoma.关键词：γ-mercaptopropyl-modified silica gel;Coptidis Rhizoma;heavy metals;cadmium
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发布时间：2024-09-23 - 摘要：Objective: To evaluate the feasibility of removing heavy metal ions from the extract of Chinese herbal medicines containing alkaloids by γ-mercaptopropyl-modified silica gel(MPS). Method: Some influence factors on removal of cadmium ion in the aqueous extract of Coptidis Rhizoma were studied, such as the amount of MPS, flow velocity, ratio of column diameter to height and temperature. The chemical changes were evaluated by the HPLC fingerprint profiles and dried extract content, before and after the removal of cadmium ion. Result: The optimum parameters were as follows:the ratio of medicinal herb to MPS 40: 1, 1: 3 of ratio of diameter to height, 10 BV · h-1 flow velocity,and the room temperature. The removing rate of cadmium ion and copper ion reached 81.3% and 35.9%, respectively. The content of magnesium and zinc ion hardly changed. Dried extract content of aqueous solution of Coptidis Rhizoma decreased by 1.3%, and the similarity of HPLC fingerprint profiles was 0.99. Conclusion: MPS can remove efficiently heavy metal ions and there was no significant change on the chemical components of aqueous solution of Coptidis Rhizoma.关键词：γ-mercaptopropyl-modified silica gel;Coptidis Rhizoma;heavy metals;cadmium
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发布时间：2024-09-23 - 摘要：Objective:To study on characteristics of in vitro release and transdermal penetration of Danhuang gel.Method:Modified Franz diffusion cell was used,with berberine and chrysophanol as indexes,in vitro drug release and percutaneous penetration laws of index components were described by kinetic model.Result:Release of chrysophanol could be fitted best by zero-power kinetics model,but berberine by Ritger-Peppas model;Penetration of chrysophanol and berberine in vitro skin were best by fitting for one-power kinetics model;Relevant linear equation of in vitro release and transdermal penetration for chrysophanol could be described as Y=0.468 7X+4.972 2（r=0.966 4）,berberine as Y=0.666 9X+2.487 4（r=0.974 0）,and in vitro release rate was higher than in vitro transdermal rate of two index components in Danhuang gel.Conclusion:In vitro release and transdermal penetration of berberine and chrysophanol had a good linear correlation.关键词：Danhuang gel;in vitro release;transdermal permeability;chrysophanol;berberine
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发布时间：2024-09-23 - 摘要：Objective: To study on characteristics of in vitro release and transdermal penetration of Danhuang gel. Method: Modified Franz diffusion cell was used,with berberine and chrysophanol as indexes,in vitro drug release and percutaneous penetration laws of index components were described by kinetic model. Result: Release of chrysophanol could be fitted best by zero-power kinetics model,but berberine by Ritger-Peppas model;Penetration of chrysophanol and berberine in vitro skin were best by fitting for one-power kinetics model;Relevant linear equation of in vitro release and transdermal penetration for chrysophanol could be described as Y=0.468 7X+4.972 2 (r=0.966 4), berberine as Y=0.666 9X+2.487 4 (r=0.974 0),and in vitro release rate was higher than in vitro transdermal rate of two index components in Danhuang gel. Conclusion: In vitro release and transdermal penetration of berberine and chrysophanol had a good linear correlation.关键词：Danhuang gel;in vitro release;transdermal permeability;chrysophanol;berberine
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发布时间：2024-09-23 - 摘要：Objective: To optimize microwave-assisted semi-bionic extraction technology of Ampelopsis grossedentata. Method: With ampelopsin as index which was determined by HPLC,extraction technology parameters was optimized by uniform design. Result: Optimum extraction technology were:pH values of water in three times for extracting were 6.0,7.5,9.0,microwave processing time of 3,1.5,1.5 min, respectively. Conclusion: Microwave-assisted semi-bionic extraction technology could effectively and quickly extract effective components from A. grossedentata.关键词：semi-bionic extraction;microwave-assisted;Ampelopsis grossedentata;ampelopsin;uniform design
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发布时间：2024-09-23 - 摘要：Objective:To optimize microwave-assisted semi-bionic extraction technology of Ampelopsis grossedentata.Method:With ampelopsin as index which was determined by HPLC,extraction technology parameters was optimized by uniform design.Result:Optimum extraction technology were:pH values of water in three times for extracting were 6.0,7.5,9.0,microwave processing time of 3,1.5,1.5 min,respectively.Conclusion:Microwave-assisted semi-bionic extraction technology could effectively and quickly extract effective components from A.grossedentata.关键词：semi-bionic extraction;microwave-assisted;Ampelopsis grossedentata;ampelopsin;uniform design
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发布时间：2024-09-23 - 摘要：Objective:To study on intestinal absorption characteristics of total saponins from Pulsatilla chinensis.Method:Isolated everted gut sac model was used to study on intestinal absorption of total saponins from P.chinensis in different segments and different drug concentration,concentration of ivy sapogenin 3-O-α-L-rhamnopyranosyl-（1→2）-[β-D-glucopyranosyl-（1→4）]-L-pyran Arab glucoside in samples were determined by HPLC.Result:Absorption of index components for total saponins from P.chinensis in different intestinal segments had no significant difference,every intestine segments cumulative absorption increased along with concentration of total saponins from P.chinensis.Conclusion:Absorption of total saponins from P.chinensis may be passive diffusion absorption and didn’t be a special absorption window.关键词：total saponins from Pulsatilla chinensis;everted intestine method;intestine absorption;HPLC
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发布时间：2024-09-23 - 摘要：Objective: To optimize extraction technology of astragaloside Ⅳ from Astragalus membranaceus by response surface methodology(RSM). Method: Three-factor and three-level Box-Behnken experimental design was employed with ethanol concentration,flow rate and liquid-solid ratioas independence variables,extraction rate of astragaloside Ⅳ as dependent variable which was determined by HPLC-ELSD,experimental data obtained by multiple regression and binomial fitting of levels from independent variables, ethanol percocation extraction technology was optimized by RSM,and to predictive analysis. Result: Optimum extraction conditions were as follows:30% ethanol as solvent, flow rate 1.9 mL · min-1 · cm-2,liquid-solid ratio 6.5 mL · g-1.Under these conditions,theoretical value of extraction rate of astragaloside Ⅳ 1.50 mg · g-1,but measured value of 1.487 mg · g-1,it showed optimized technology model fitted well. Conclusion: Box-Behnken experimental design could be used for extraction optimization of percocation extraction process for A. membranaceus,optimized technology was simple and stable.关键词：astragaloside Ⅳ;Box-Behnken experimental design;response surface methodology;HPLC-ELSD
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction technology of astragaloside Ⅳ from Astragalus membranaceus by response surface methodology（RSM）.Method:Three-factor and three-level Box-Behnken experimental design was employed with ethanol concentration,flow rate and liquid-solid ratioas independence variables,extraction rate of astragaloside Ⅳ as dependent variable which was determined by HPLC-ELSD,experimental data obtained by multiple regression and binomial fitting of levels from independent variables,ethanol percocation extraction technology was optimized by RSM,and to predictive analysis.Result:Optimum extraction conditions were as follows:30% ethanol as solvent,flow rate 1.9 mL · min-1 · cm-2,liquid-solid ratio 6.5 mL · g-1.Under these conditions,theoretical value of extraction rate of astragaloside Ⅳ 1.50 mg · g-1,but measured value of 1.487 mg · g-1,it showed optimized technology model fitted well.Conclusion:Box-Behnken experimental design could be used for extraction optimization of percocation extraction process for A.membranaceus,optimized technology was simple and stable.关键词：astragaloside Ⅳ;Box-Behnken experimental design;response surface methodology;HPLC-ELSD
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发布时间：2024-09-23 - 摘要：Objective: To optimize ultrasonic-assisted extraction technology of total flavonoids from Penthorum chinense and evaluate its in vitro antioxidant activity. Method: With yield of total flavonoids as index,the concentration of ethanol,extraction time, liquid-material ratio were optimized by response surface methodology based on single-factor tests,and its in vitro antioxidant activity were evaluated by multi-test systems. Result: Optimal extraction technology conditions were:extracted 20 min with 26 times the amount of 60% ethanol at 50℃. Under these conditions,yield of total flavonoids reached 4.14%, which was agreed with model predictions.In vitro antioxidant activities results showed that total flavonoids from P. chinense had good antioxidant activities of scavenging DPPH free radical and hydroxyl free radical, but also had strong reducing power and total antioxidant capability. Conclusion: Optimized ultrasonic-assisted extraction technology was simple, rapid and efficient.Total flavonoids from P. chinense had good antioxidant activity which was worth of further study.关键词：Penthorum chinense;total flavonoids;ultrasonic-assisted extraction;response surface methodology;antioxidant activity
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发布时间：2024-09-23 - 摘要：Objective:To optimize ultrasonic-assisted extraction technology of total flavonoids from Penthorum chinense and evaluate its in vitro antioxidant activity.Method:With yield of total flavonoids as index,the concentration of ethanol,extraction time,liquid-material ratio were optimized by response surface methodology based on single-factor tests,and its in vitro antioxidant activity were evaluated by multi-test systems.Result:Optimal extraction technology conditions were:extracted 20 min with 26 times the amount of 60% ethanol at 50 ℃.Under these conditions,yield of total flavonoids reached 4.14%,which was agreed with model predictions.In vitro antioxidant activities results showed that total flavonoids from P.chinense had good antioxidant activities of scavenging DPPH free radical and hydroxyl free radical,but also had strong reducing power and total antioxidant capability.Conclusion:Optimized ultrasonic-assisted extraction technology was simple,rapid and efficient.Total flavonoids from P.chinense had good antioxidant activity which was worth of further study.关键词：Penthorum chinense;total flavonoids;ultrasonic-assisted extraction;response surface methodology;antioxidant activity
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发布时间：2024-09-23 - 摘要：Objective:To optimize water extraction and alcohol precipitation technology for Pingxiao granule.Method:With solid ratio,total extraction rate of ephedrine hydrochloride and paeoniflorin as comprehensive evaluation indexes,orthogonal test was used to optimize extraction technology with soaking time,the amount of water,decocting times and decocting time as factors;With total transfer rate of ephedrine hydrochloride and paeoniflorin as index,effect of solid-liquid ratio,alcohol precipitation time and the concentration of ethanol on alcohol precipitation technology was optimized by orthogonal test.Result:Optimum water extraction process was as follows:decocted 3 times with 8 times the amount water,1 h each time;Optimum alcohol precipitation process was:the concentration of decoction liquid concentrated 2 g · mL-1,precipitated to the concentration of ethanol 60%,stirred well,stilled 8 h.Conclusion:These optimized technology could ensure effective components saved to the maximum,it could be used for industrial production.关键词：Pingxiao granule;extraction process;alcohol precipitation process;orthogonal test;ephedrine hydrochlorid;paeoniflorin
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发布时间：2024-09-23 - 摘要：Objective: To optimize water extraction and alcohol precipitation technology for Pingxiao granule. Method: With solid ratio,total extraction rate of ephedrine hydrochloride and paeoniflorin as comprehensive evaluation indexes,orthogonal test was used to optimize extraction technology with soaking time,the amount of water,decocting times and decocting time as factors;With total transfer rate of ephedrine hydrochloride and paeoniflorin as index,effect of solid-liquid ratio,alcohol precipitation time and the concentration of ethanol on alcohol precipitation technology was optimized by orthogonal test. Result: Optimum water extraction process was as follows:decocted 3 times with 8 times the amount water,1 h each time;Optimum alcohol precipitation process was:the concentration of decoction liquid concentrated 2 g · mL-1,precipitated to the concentration of ethanol 60%,stirred well,stilled 8 h. Conclusion: These optimized technology could ensure effective components saved to the maximum, it could be used for industrial production.关键词：Pingxiao granule;extraction process;alcohol precipitation process;orthogonal test;ephedrine hydrochlorid;paeoniflorin
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发布时间：2024-09-23 - 摘要：Objective: To analyze transdermal effect and transdermal composition of volatile oil from Notopterygium incisum,Angelica pubescens and pair of them by GC-MS. Method: Chemical constituents of volatile oil in in vitro transdermal donor cell and receiving cell were identified by GC-MS,The relative contents in percentage were calculated with area normalization method.According to mathematical derivation,relative contents was changed into translation rate,which reflected transdermal effect of every components. Result: After percutaneous absorption, thirty-nine components were identified in N. incisum (93.22%),twenty-nine in A. pubescens (93.74%) and thirty-six in drug pair of them (92.75%).Main components of percutaneous absorption in N. incisum were 1-terpineol (30.96%),4-terpineol (28.22%),borneol(7.10%),etc;Main components of percutaneous absorption in A. pubescens were o-acetyl-p-methylbenzyl alcohol (55.70%), trans-oxy-linalool (6.79%),1,7,7-trimethyl-bicyclic 5-hexene-2-one(4.78%),etc;Main components of percutaneous absorption in drug pair of them were 1-terpineol (28.86%), 4-terpineol (22.41%), o-acetyl-p-methylbenzyl alcohol (12.15%), borneol (5.62%),etc. Conclusion: Transmission rate of every components in three kinds of volatile oil could be sorted qualitatively of transdermal effect.Transmission rate of 1-terpineol was the most in volatile oil of N. incisum. 1,7,7-trimethyl-bicyclic 5-hexene-2-one had the most transmission rate in volatile oil of A. pubescens. 1-terpineol had the most transmission ratio in volatile oil of drug pair of them.关键词：Notopterygium incisum;Angelica pubescens;volatile oil;GC-MS;transmission rate
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发布时间：2024-09-23 - 摘要：Objective:To analyze transdermal effect and transdermal composition of volatile oil from Notopterygium incisum,Angelica pubescens and pair of them by GC-MS.Method:Chemical constituents of volatile oil in in vitro transdermal donor cell and receiving cell were identified by GC-MS,The relative contents in percentage were calculated with area normalization method.According to mathematical derivation,relative contents was changed into translation rate,which reflected transdermal effect of every components.Result:After percutaneous absorption,thirty-nine components were identified in N.incisum（93.22%）,twenty-nine in A.pubescens（93.74%） and thirty-six in drug pair of them（92.75%）.Main components of percutaneous absorption in N.incisum were 1-terpineol（30.96%）,4-terpineol（28.22%）,borneol（7.10%）,etc;Main components of percutaneous absorption in A.pubescens were o-acetyl-p-methylbenzyl alcohol（55.70%）,trans-oxy-linalool（6.79%）,1,7,7-trimethyl-bicyclic 5-hexene-2-one（4.78%）,etc;Main components of percutaneous absorption in drug pair of them were 1-terpineol（28.86%）,4-terpineol（22.41%）,o-acetyl-p-methylbenzyl alcohol（12.15%）,borneol（5.62%）,etc.Conclusion:Transmission rate of every components in three kinds of volatile oil could be sorted qualitatively of transdermal effect.Transmission rate of 1-terpineol was the most in volatile oil of N.incisum.1,7,7-trimethyl-bicyclic 5-hexene-2-one had the most transmission rate in volatile oil of A.pubescens.1-terpineol had the most transmission ratio in volatile oil of drug pair of them.关键词：Notopterygium incisum;Angelica pubescens;volatile oil;GC-MS;transmission rate
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发布时间：2024-09-23 - 摘要：Objective:To optimize softening and cutting technology of Coptis chinensis.Method:With the content of epiberberine,coptisine,palmatine and berberine as indexes,which were determined by HPLC,softening and cutting technology of C.chinensis was optimized by single-factor test and orthogonal test.Result:Spraying was the best softening method,C.chinensis was sprayed in 48 h with 60 L water per 100 kg at room temperature medicinal herbs.Optimum cutting technology was:cutted C.chinensis into 1-2 mm piece.Conclusion:These optimized process were feasible,and could be promoted in industrial production.关键词：Coptis chinensis;softening;cutting;HPLC;orthogonal test
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发布时间：2024-09-23 - 摘要：Objective: To optimize softening and cutting technology of Coptis chinensis. Method: With the content of epiberberine,coptisine,palmatine and berberine as indexes,which were determined by HPLC,softening and cutting technology of C. chinensis was optimized by single-factor test and orthogonal test. Result: Spraying was the best softening method,C. chinensis was sprayed in 48 h with 60 L water per 100 kg at room temperature medicinal herbs. Optimum cutting technology was:cutted C. chinensis into 1-2 mm piece. Conclusion: These optimized process were feasible,and could be promoted in industrial production.关键词：Coptis chinensis;softening;cutting;HPLC;orthogonal test
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发布时间：2024-09-23 - 摘要：Objective: To study on separation and purification feasible process of polysaccharides from R. palmatum by ultrafiltration. Method: Centrifugation conditions were optimized by orthogonal test before ultrafiltration, ultrafiltration technology parameters were optimized by uniform design with yield and concent of polysaccharides as indexes,and ultrafiltration pressure,liquid temperature and liquid concentration were selected as investigated factors. Result: Optimum ultra-filtration process was:centrifuged 12 min with 3 000 r · min-1,membrane with 105 molecular interception,ultrafiltration pressure 0.05-0.09 MPa, liquid temperature 40℃, ratio of solid-liquid 1: 10-1: 30, pH 6.0,ultrafiltration membrane was pretreated 10 min with tween-80 (5 g ·L-1). Conclusion: Optimized ultrafiltration process could obtain higher yield and concent of polysaccharides while compared with non-ultrafiltration. Ultrafiltration technology could be used for purification and enrichment of polysaccharides from R. palmatum with industrial development prospect.关键词：polysaccharides from Rheum palmatum;ultra-filtration;purification process
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发布时间：2024-09-23 - 摘要：Objective:To study on separation and purification feasible process of polysaccharides from R.palmatum by ultrafiltration.Method:Centrifugation conditions were optimized by orthogonal test before ultrafiltration,ultrafiltration technology parameters were optimized by uniform design with yield and concent of polysaccharides as indexes,and ultrafiltration pressure,liquid temperature and liquid concentration were selected as investigated factors.Result:Optimum ultra-filtration process was:centrifuged 12 min with 3 000 r · min-1,membrane with 105 molecular interception,ultrafiltration pressure 0.05-0.09 MPa,liquid temperature 40 ℃,ratio of solid-liquid 1∶10-1∶30,pH 6.0,ultrafiltration membrane was pretreated 10 min with tween-80（5 g·L-1）.Conclusion:Optimized ultrafiltration process could obtain higher yield and concent of polysaccharides while compared with non-ultrafiltration.Ultrafiltration technology could be used for purification and enrichment of polysaccharides from R.palmatum with industrial development prospect.关键词：polysaccharides from Rheum palmatum;ultra-filtration;purification process
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- 266
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发布时间：2024-09-23 - 摘要：Objective:To optimize extraction and purification technology of Qionggui Shuloug granule.Method:With transfer rate of ferulic acid as index,water extraction technology of Qionggui Shuloug granule was optimized by orthogonal test,soaking time,extraction times,extraction time and the amount of water were selected as factors,the content of ferulic acid was determined by HPLC;With transfer rate and purity of ferulic acid as indexes,single-factor test was used to investigate alcohol precipitation technology.Result:Optimized extraction technology was:soaked 1 h with 15 times the amount of water,extracted 2 times with 1 h per time;alcohol precipitation technology was:alcohol precipitation to the concentration of ethanol 60%,the relative density of 1.08-1.14（60 ℃）.Conclusion:This optimized technology was stable and feasible,it could provide experimental basis for industrial production of Qionggui Shuloug granule.关键词：Qionggui Shuloug granule;ferulic acid;alcohol precipitation;extraction
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- 43
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- 1
发布时间：2024-09-23 - 摘要：Objective: To optimize extraction and purification technology of Qionggui Shuloug granule. Method: With transfer rate of ferulic acid as index,water extraction technology of Qionggui Shuloug granule was optimized by orthogonal test,soaking time,extraction times,extraction time and the amount of water were selected as factors,the content of ferulic acid was determined by HPLC;With transfer rate and purity of ferulic acid as indexes,single-factor test was used to investigate alcohol precipitation technology. Result: Optimized extraction technology was:soaked 1 h with 15 times the amount of water,extracted 2 times with 1 h per time;alcohol precipitation technology was:alcohol precipitation to the concentration of ethanol 60%,the relative density of 1.08-1.14(60℃). Conclusion: This optimized technology was stable and feasible,it could provide experimental basis for industrial production of Qionggui Shuloug granule.关键词：Qionggui Shuloug granule;ferulic acid;alcohol precipitation;extraction
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发布时间：2024-09-23 - 摘要：Objective:To optimize one-step granulation technology for Xinjianwei package tablet.Method: With particle hardness,particle size,compressibility,properties and the content of sodium bicarbonate in tablet core as indexes,single-factors test was used to investigate key factors on granulation technology for Xinjianwei package tablet.Result: Granulation technology of Xinjianwei package tablet didn’t change original materials,material temperature was 45 ℃,inlet air temperature was 50-60 ℃,5% starch slurry as adhesive,under these conditions,prepared particle had excellent compressibility and mobility,package tablet was stability,and the content of sodium bicarbonate had been raised.Conclusion:One-step granulation had very excellent operability,quality of products was stability and was improved,it could provided reference for mass production.关键词：Xinjianwei package tablet;one-step granulation;technology optimization
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- 181
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发布时间：2024-09-23 - 摘要：Objective: To optimize one-step granulation technology for Xinjianwei package tablet. Method: With particle hardness,particle size,compressibility,properties and the content of sodium bicarbonate in tablet core as indexes,single-factors test was used to investigate key factors on granulation technology for Xinjianwei package tablet. Result: Granulation technology of Xinjianwei package tablet didn't change original materials,material temperature was 45℃,inlet air temperature was 50-60℃,5% starch slurry as adhesive,under these conditions, prepared particle had excellent compressibility and mobility, package tablet was stability,and the content of sodium bicarbonate had been raised. Conclusion: One-step granulation had very excellent operability, quality of products was stability and was improved,it could provided reference for mass production.关键词：Xinjianwei package tablet;one-step granulation;technology optimization
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》由国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的学术刊物,已成为"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊";"中国学术期刊综合评价
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- 5
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发布时间：2024-09-23 - 摘要：Objective: To study the rule of the dynamic HPLC fingerprints of safflower from different source and find a comprehensive evaluation method suitable for quality control of TCM(or compounds) multicomponent. Method: Thirty-two batches safflower sample were collected and safflower yellow A was used for reference. RP-HPLC was used to establish chromatographic fingerprints for evaluated and analyzed by total quantum statistical moment similarity (TQSMS) and principal component analysis (PCA). Result: Twenty samples in 32 comply with Chinese pharmacopoeia; the TQSMPM parameters of the AUCT, MCRTT, VCRTT were 4 454 mV · s-1,27.89 min,305.49 min2,and the RSD is 22%,4.69%,7.32% respectively for all. The 20 samples up to standard safflower were analyzed by TQSMS:37 common characteristic peaks, the similarity range of 0.82-0.99 contrasts to the number 5 samples, the average similarity was 0.94, RSD was 5.03%. Seven principal components which accounted for over 88.72% of the total variance were extracted from the original data. Conclusion: TQSMS combined with PCA can comprehensively analyze the quality and dynamic regulation of safflower, and it is an effective method that is fit to the quality evaluation of chemical components group of Chinese medicine.关键词：Carthamus tinctorius L.;multicomponent;dynamic fingerprint;comprehensive evaluation;similarity
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发布时间：2024-09-23 - 摘要：Objective: To develop an HPLC method for simultaneous determination of uridine, adenosine, guanosine and epigoitrin in Radix Isatidis, and compare the content variation of the four components of herbs harvested at different time. Method: Four components were separated by Kromasil C18 column (4.6 mm×250 mm, 5 μm) with gradient mobile phase of methanol-0.1% phosphoric acid at the flow rate of 0.7 mL ·min-1. The detection wavelength was set at 254 nm (0-30 min) and 245 nm (30-45 min). The column temperature was maintained at 25℃. Result: The calibration curves of uridine, adenosine, guanosine and epigoitrin were in good linearity over the range of 14.80-1 184 ng (r=0.999 9), 14.55-1 164 ng (r=0.999 9), 13.75-1 100 ng (r=0.999 9), 12.25-980 ng (r=0.999 9), respectively. The average recoveries of the four components were 99.5% (RSD 1.2%), 98.3% (RSD 1.4%), 98.1% (RSD 1.2%), 99.0% (RSD 0.9%), respectively. The content of four components in Radix Isatidis varied with different harvest time. Conclusion: The method is simple, accurate and reproducible for quality control of Radix Isatidis.关键词：Radix Isatidis;uridine;adenosine;guanosine;epigoitrin;HPLC
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发布时间：2024-09-23 - 摘要：<正>《中国中药杂志》系中国科协主管,中国药学会主办,中国中医科学院中药研究所承办的综合性中药学术期刊。创刊于1955年7月,是创刊最早、发行量最大的中药学术刊物。《中国中药杂志》全面反映我国中医科研最高学术水平,主要报道该领域新成果、新技术、新方法与新思路,内容包括栽培、资源与鉴定、炮制、药剂、化学、药理、不良反应、临床等。设有专论、综
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- 6
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发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC method for content determination of 5-hydroxymethylfurfural (5-HMF) in commercial Trichosanthis Fructus, a commonly used Chinese material medica. Method: The HPLC analysis was performed on an ODS column (4.6 mm×250 mm, 5 μm) and the mobile phase consisted of methanol-water (15: 85) at a flow rate of 0.8 mL · min-1. The detective wavelength was set at 284 nm and the column temperature was maiutain at 30℃. Result: The HPLC method for content determination of 5-HMF in Trichosanthis Fructus was established. 5-HMF was detected in 18 batches of commercial Trichosanthis Fructus, the content of which were in the range of 11.10-193.6 μg ·g-1. Conclusion: The content of 5-HMF in commercial Trichosanthis Fructus is quite different. This method is suitable for the quality control of Trichosanthis Fructus.关键词：Trichosanthis Fructus;5-hydroxymethylfurfural (5-HMF);HPLC;content determination;Maillard reaction
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发布时间：2024-09-23 - 摘要：<正>《中国中医药信息杂志》是由国家中医药管理局主管、中国中医科学院中医药信息研究所主办的中医药学术期刊。本刊立足于行业报道的前沿,关注相关的政策动态,跟踪报道中医药重大课题,及时分析报道中医药的新政策、新技术、新发明、新成果、新疗法,努力使信息的选择与表达方式能够充分体现中医药发展水平,为广大读者提供一流的信息服务。
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- 4
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发布时间：2024-09-23 - 摘要：Objective: To establish a method for determination of total flavonoids in Polygonum perfoliatum. Method: Total flavoaoids were determined by UV-Vis spectrophotometer with rutiu as reference substance and 0.1 mol · L-1 AlCl3, 1 mol · L-1 NaAc as chromogenic agent; the detection wavelength was at 413 nm. Result: A good linear relationship was achieved over the concentration range of 6.24-21.84 μg(r =0.999 8) for Rutin. The regression equation of total flavonoids was Y=38.183X-0.001, with an average recovery of 97.4%, RSD 2.2%. Conclusion: The method is simple and stable, and it is suitable for the content determination of total flavonoids from P. perfoliatum.关键词：Polygonum perfoliatum;total flavonoids;colorimetry;the way of coloration;extraction;UV-Vis spectrophotometer
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- 0
发布时间：2024-09-23 - 摘要：<正>《天津中医药》（原名《天津中医》）创刊于1984年,是由天津市卫生局主管、天津中医药大学、天津中医药学会和天津中西医结合学会主办的综合性中医药学术期刊。本刊继承与发展并重,中医与中药兼顾,理论与实践并举,坚持中医特色,内容
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- 4
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发布时间：2024-09-23 - 摘要：Objective: To establish an HPLC-ELSD method for the simultaneous determination of the content of chikusetsusaponin Ⅳa and 3β -([[BFQ]O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus. Method: The RP-HPLC-ELSD method was used. Chromatographic conditions:AlltimaTM-C18 chromatographic column (4.6 mm×250 mm, 5 μm);acetonitrile(A)-0.5% acetic acid water(B) as mobile phase;column temperature at 35℃. ELSD detector conditions:drift tube temperature: 106℃;carrier gas(nitrogen) flow rate: 2.9 L · min-1;amplification factor:1;impactor:close. Result: The calibration curves showed good linear relationship in the range from 0.535 to 8.560 μg (r=0.996 5) for chikusetsusaponin Ⅳa and 0.515 to 8.240 μg (r=0.998 7) for 3β-( oxy)-olean-12-ene-28-olc acid. The average recovery rates(n=6) were 98.39% and 98.97%, and RSDs were 1.50% and 1.59%. Conclusion: This method is accurate, sensitive and can be used to determine the content of chikusetsusaponin Ⅳa and 3β-( oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.关键词：Acanthopanax gracilistylus;fruits;triterpenoidal saponins;HPLC-ELSD;determination of content
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发布时间：2024-09-23 - 摘要：<正>《中国实验方剂学杂志》系国家中医药管理局主管,中国中医科学院中药研究所和中国中西医结合学会中药专业委员会主办的专业性学术刊物,系"中国科技论文统计源期刊"（中国科技核心期刊）、"中国中文核心期刊"、"万方数据—数字化期刊群"、"中国学术期刊综合评价数据库来源"期刊、"中国期刊网、中国学术
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- 14
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发布时间：2024-09-23 - 摘要：Objective:To study the rule of the dynamic HPLC fingerprints of safflower from different source and find a comprehensive evaluation method suitable for quality control of TCM（or compounds） multicomponent.Method:Thirty-two batches safflower sample were collected and safflower yellow A was used for reference.RP-HPLC was used to establish chromatographic fingerprints for evaluated and analyzed by total quantum statistical moment similarity（TQSMS） and principal component analysis（PCA）.Result:Twenty samples in 32 comply with Chinese pharmacopoeia;the TQSMPM parameters of the AUCT,MCRTT,VCRTT were 4 454 mV · s-1,27.89 min,305.49 min2,and the RSD is 22%,4.69%,7.32% respectively for all.The 20 samples up to standard safflower were analyzed by TQSMS:37 common characteristic peaks,the similarity range of 0.82-0.99 contrasts to the number 5 samples,the average similarity was 0.94,RSD was 5.03%.Seven principal components which accounted for over 88.72% of the total variance were extracted from the original data.Conclusion:TQSMS combined with PCA can comprehensively analyze the quality and dynamic regulation of safflower,and it is an effective method that is fit to the quality evaluation of chemical components group of Chinese medicine.关键词：Carthamus tinctorius L.;multicomponent;dynamic fingerprint;comprehensive evaluation;similarity
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- 333
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- 6
发布时间：2024-09-23 - 摘要：Objective: The aroma compounds in Huoshanhuangya Tea were studied. Method: The aroma compounds were analyzed by CTC head-space solid micro-extraction, coupled with GC-MS for the first time. A quantitative analysis in percent was performed by peak area normalization measurements. Result: One hundred and three compounds were separated and 30 compounds were identified, representative of 44.85% of total aroma compounds. The main compounds were 2,6-bis(1,1-dimethylethyl)-4-(1-oxopropyl) phenol (9.56%) and 3,7-dimethyl-1,6-octadien-3-ol (5.07%). Conclusion: The main aroma compounds in Huoshanhuangya Tea were aliphatic hydrocarbon and aromatic compounds.关键词：Huoshanhuangya Tea;CTC-HS-SPME-GC/MS;aroma components
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- 0
发布时间：2024-09-23 - 摘要：Objective:To develop an HPLC method for simultaneous determination of uridine,adenosine,guanosine and epigoitrin in Radix Isatidis,and compare the content variation of the four components of herbs harvested at different time.Method:Four components were separated by Kromasil C18 column（4.6 mm×250 mm,5 μm） with gradient mobile phase of methanol-0.1% phosphoric acid at the flow rate of 0.7 mL ·min-1.The detection wavelength was set at 254 nm（0-30 min） and 245 nm（30-45 min）.The column temperature was maintained at 25 ℃.Result:The calibration curves of uridine,adenosine,guanosine and epigoitrin were in good linearity over the range of 14.80-1 184 ng（r =0.999 9）,14.55-1 164 ng（r=0.999 9）,13.75-1 100 ng（r=0.999 9）,12.25-980 ng（r=0.999 9）,respectively.The average recoveries of the four components were 99.5%（RSD 1.2%）,98.3%（RSD 1.4%）,98.1%（RSD 1.2%）,99.0%（RSD 0.9%）,respectively.The content of four components in Radix Isatidis varied with different harvest time.Conclusion:The method is simple,accurate and reproducible for quality control of Radix Isatidis.关键词：Radix Isatidis;uridine;adenosine;guanosine;epigoitrin;HPLC
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- 159
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- 3
发布时间：2024-09-23 - 摘要：Objective: To establish the method for determining the content of berberine in traditional Chinese medicine with online solid phase extraction-HPLC. Method: Using one of the dual gradient pumps to enrich and purify the sample solution, then wash the aimed analyte into analytical column by switching the valve to another position after removing of matrix interference substance. Silica-based strong cation exchange column (300 Å, 4.6 mm×50 mm) was used as online-SPE column with a mobile phase consisting of acetonitrile-500 mmol ·L-1 ammonium acetate in a gradient elution. Acclaim C18(4.6 mm×250 mm, 5 μm) was used as analytical column with a mobile phase of acetonitrile-30 mmol ·L-1 KH2PO4 at a flow rate of 1.0 mL · min-1. UV detection wavelength was set up at 345 nm. Result: There is a good linear relationship(r=0.999) within the concentration range of 1.24-124 mg · L-1. The average recovery was 99.5%. Conclusion: This method was simple, fast and has a better speciality. The results of determination were reliable and reproducible by validating for accuracy, precision and reproducibility.
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC method for content determination of 5-hydroxymethylfurfural（5-HMF） in commercial Trichosanthis Fructus,a commonly used Chinese material medica.Method:The HPLC analysis was performed on an ODS column（4.6 mm×250 mm,5 μm） and the mobile phase consisted of methanol-water（15∶85） at a flow rate of 0.8 mL · min-1.The detective wavelength was set at 284 nm and the column temperature was maiutain at 30 ℃.Result:The HPLC method for content determination of 5-HMF in Trichosanthis Fructus was established.5-HMF was detected in 18 batches of commercial Trichosanthis Fructus,the content of which were in the range of 11.10-193.6 μg·g-1.Conclusion:The content of 5-HMF in commercial Trichosanthis Fructus is quite different.This method is suitable for the quality control of Trichosanthis Fructus.关键词：Trichosanthis Fructus;5-hydroxymethylfurfural（5-HMF）;HPLC;content determination;Maillard reaction
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- 385
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- 27
发布时间：2024-09-23 - 摘要：Objective: To investigate the content differences of four effective components in different parts of seeds of Euphorbia lathyris in different areas and to be used for total quality control of seeds of E. lathyris. Method: An RP-HPLC method had been developed for detecting. The system consisting of an Apollo C18 column (4.6 mm×250 mm,5 μm) and a mixture of acetonitrile and 0.1% phosphate acid as the mobile phase in gradient mode was adopted. The detection wavelength was set at 280 nm and the column temperature was room temperature. Result: The linear response range was 3.23-162 mg ·L-1(r =0.999 8) for aesculetin, 3.02-152 mg ·L-1(r=1.000 0) for Euphorbia factor L1, 1.84-91.8 mg ·L-1(r=0.999 9) for Euphorbia factor L2 and 3.64-182 mg ·L-1(r=1.000 0) for Euphorbia factor L3.The average recoveries of these components were 98.2% (RSD 1.9%), 99.8% (RSD 2.3%), 100.9% (RSD 1.9%) and 101.9% (RSD 2.5%), respectively. Conclusion: The method is simple, accurate and reproducible for quality control of seeds of E. lathyris and can be used for comparing the four effective components of seeds of E. lathyris of different parts in different areas.关键词：seeds of Euphorbia lathyris;HPLC;effective components;different parts
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发布时间：2024-09-23 - 摘要：Objective:To establish a method for determination of total flavonoids in Polygonum perfoliatum.Method:Total flavoaoids were determined by UV-Vis spectrophotometer with rutiu as reference substance and 0.1 mol · L-1 AlCl3,1 mol · L-1 NaAc as chromogenic agent;the detection wavelength was at 413 nm.Result:A good linear relationship was achieved over the concentration range of 6.24-21.84 μg（r =0.999 8） for Rutin.The regression equation of total flavonoids was Y=38.183X-0.001,with an average recovery of 97.4%,RSD 2.2%.Conclusion:The method is simple and stable,and it is suitable for the content determination of total flavonoids from P.perfoliatum.关键词：Polygonum perfoliatum;total flavonoids;colorimetry;the way of coloration;extraction;UV-Vis spectrophotometer
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- 447
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- 26
发布时间：2024-09-23 - 摘要：Objective: To establish assaying methods for the determination of quercetin and kaempferol in Silkworm Powder. Method: The content of quercetin and kaempferol was determined by RP-HPLC, using a KromasiL-C18 column (4.6 mm×250 mm, 5 μm). The column temperature was fixed on room temperature. Methanol-0.4% phosphoric acid (50: 50) was used as mobile phase, flow-rate was 1.0 mL · min-1.The detecting wavelength was set at 360 nm. Result: The linear range were 0.012 5-0.4 μg (r=0.999 8) and 0.014-0.448 μg (r=0.999 9). The average recoveries were 97.53% and 100.3%, with the RSD of 1.9% and 1.0%. Conclusion: The method established is rapid and accurate, which could be used for quality control of Silkworm Powder.关键词：Silkworm Powder;quercetin;Kaempferol;RP-HPLC
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an HPLC-ELSD method for the simultaneous determination of the content of chikusetsusaponin Ⅳa and 3β-（[O-β-D-glucopyranuronosyl]oxy）-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.Method:The RP-HPLC-ELSD method was used.Chromatographic conditions:AlltimaTM-C18 chromatographic column（4.6 mm×250 mm,5 μm）;acetonitrile（A）-0.5% acetic acid water（B） as mobile phase;column temperature at 35 ℃.ELSD detector conditions:drift tube temperature:106 ℃;carrier gas（nitrogen） flow rate:2.9 L · min-1;amplification factor:1;impactor:close.Result:The calibration curves showed good linear relationship in the range from 0.535 to 8.560 μg（r=0.996 5） for chikusetsusaponin Ⅳa and 0.515 to 8.240 μg（r=0.998 7） for 3β-（[O-β-D-glucopyranuronosyl]oxy）-olean-12-ene-28-olc acid.The average recovery rates（n=6） were 98.39% and 98.97%,and RSDs were 1.50% and 1.59%.Conclusion:This method is accurate,sensitive and can be used to determine the content of chikusetsusaponin Ⅳa and 3β-（[O-β-D-glucopyranuronosyl]oxy）-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.关键词：Acanthopanax gracilistylus;fruits;triterpenoidal saponins;HPLC-ELSD;determination of content
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- 175
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- 4
发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for determining puerarin in Chaiqin soft capsules. Method: The HPLC analysis was performed on a C18 column (4.6 mm×250 mm, 5 μm) with methanol-water-phosphoric acid (25: 75: 0.2) as mobile phase, detected at the wavelength of 250 nm. Result: Puerarinwas linear in the range of 0.559 5-111.9 mg · L-1(r=0.999 8). The average recovery was 99.85%, RSD 0.83%. the RSDs of precision and reproducibility were 0.52% and 0.57% respectively. Conclusion: The method is simple, accurate and reproducible, which can be used to determine puerarin in Chaiqin soft capsules.关键词：HPLC;Chaiqin soft capsules;puerarin
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- 0
发布时间：2024-09-23 - 摘要：Objective:The aroma compounds in Huoshanhuangya Tea were studied.Method:The aroma compounds were analyzed by CTC head-space solid micro-extraction,coupled with GC-MS for the first time.A quantitative analysis in percent was performed by peak area normalization measurements.Result:One hundred and three compounds were separated and 30 compounds were identified,representative of 44.85% of total aroma compounds.The main compounds were 2,6-bis（1,1-dimethylethyl）-4-（1-oxopropyl） phenol（9.56%） and 3,7-dimethyl-1,6-octadien-3-ol（5.07%）.Conclusion:The main aroma compounds in Huoshanhuangya Tea were aliphatic hydrocarbon and aromatic compounds.关键词：Huoshanhuangya Tea;CTC-HS-SPME-GC/MS;aroma components
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- 290
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- 13
发布时间：2024-09-23 - 摘要：Objective: Study and revise the quality standard of Zhuangyao Bushen pills. Method: Astragalus membranceus, red ginseng, Dipsacus asper, Radix Astragal,Schisandrae Chiensis Fructus in Zhuangyao Bushen pills were identified by TLC method and the content of asperosaponin Ⅵ was determined by HPLC. Result: TLC identification method for A. membranceus, Angelicae,red ginseng,dipsacus asperoides,Radix Astragal,Schisandrae Sphenantherae Fructus was simple and feasible with strong specificity. AsperosaponinⅥ content was linear in the range of 0.071 6-0.716 g · L-1;the recovery was 99.15%,RSD 1.42%. Conclusion: The method can effectively control the quality of Zhuangyao Bushen pills.关键词：Zhuangyao Bushen pills;TLC;HPLC;quality standards
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish the method for determining the content of berberine in traditional Chinese medicine with online solid phase extraction-HPLC.Method:Using one of the dual gradient pumps to enrich and purify the sample solution,then wash the aimed analyte into analytical column by switching the valve to another position after removing of matrix interference substance.Silica-based strong cation exchange column（300 ,4.6 mm×50 mm） was used as online-SPE column with a mobile phase consisting of acetonitrile-500 mmol·L-1 ammonium acetate in a gradient elution.Acclaim C18（4.6 mm×250 mm,5 μm） was used as analytical column with a mobile phase of acetonitrile-30 mmol·L-1 KH2PO4 at a flow rate of 1.0 mL · min-1.UV detection wavelength was set up at 345 nm.Result:There is a good linear relationship（r=0.999） within the concentration range of 1.24-124 mg · L-1.The average recovery was 99.5%.Conclusion:This method was simple,fast and has a better speciality.The results of determination were reliable and reproducible by validating for accuracy,precision and reproducibility.
- 0
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- 165
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- 8
发布时间：2024-09-23 - 摘要：Objective: To establish a method for determing Salvianolic acid B in Yishen Juanbi capsule. Method: Determination of salvianolic acid B in Yishen Juanbi capsule was performed on Kromasil-C18 column (4.6 mm×250 mm,5 μm); mobile phase was methanol-acetonitrile-1.7% formic acid solution(28: 8: 64); flow rate was 1.0 mL · min-1; detection wavelength was set at 286 nm. Result: Salvianolic acid B was linear in the range of 0.150 4-15.04 μg(r=0.999 9); the average recovery was 98.90%, RSD 1.22%. Conclusion: This method has good repeatability and flexibility.It can be used for quality control in production of Yishen Juanbi capsule.关键词：HPLC;Yishen Juanbi capsule;salvianolic acid B;content determination
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the content differences of four effective components in different parts of seeds of Euphorbia lathyris in different areas and to be used for total quality control of seeds of E.lathyris.Method:An RP-HPLC method had been developed for detecting.The system consisting of an Apollo C18 column（4.6 mm×250 mm,5 μm） and a mixture of acetonitrile and 0.1% phosphate acid as the mobile phase in gradient mode was adopted.The detection wavelength was set at 280 nm and the column temperature was room temperature.Result:The linear response range was 3.23-162 mg·L-1（r =0.999 8） for aesculetin,3.02-152 mg·L-1（r=1.000 0） for Euphorbia factor L1,1.84-91.8 mg·L-1（r=0.999 9） for Euphorbia factor L2 and 3.64-182 mg·L-1（r =1.000 0） for Euphorbia factor L3.The average recoveries of these components were 98.2%（RSD 1.9%）,99.8%（RSD 2.3%）,100.9%（RSD 1.9%） and 101.9%（RSD 2.5%）,respectively.Conclusion:The method is simple,accurate and reproducible for quality control of seeds of E.lathyris and can be used for comparing the four effective components of seeds of E.lathyris of different parts in different areas.关键词：seeds of Euphorbia lathyris;HPLC;effective components;different parts
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- 245
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- 7
发布时间：2024-09-23 - 摘要：Objective: To establish a HPLC method for the determination of eldrin,quertetin and kaempferol in Impatiens balsamina. Method: The determination was performed on a Diamonsil C18 column(4.6 mm×250 mm,5 μm),with a gredient mobile phase of acetonitrile-0.1% glacial acetic acid;the flow rate was 1.0 mL · min-1;column temperature was 30℃;the detection wavelength was at 260 nm. Result: The chromatographic peaks of eldrin,quertetin and kaempferol with other components were completely separated.The average recovery was 98.71%,98.96%,98.89%,RSD 2.13%,2.34%,2.38%. Conclusion: The method was rapid and accurate for quality control of I. balsamina, and laid foundations for further research and development.关键词：HPLC;Impatiens balsamina;eldrin;quertetin;kaempferol
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish assaying methods for the determination of quercetin and kaempferol in Silkworm Powder.Method:The content of quercetin and kaempferol was determined by RP-HPLC,using a KromasiL-C18 column（4.6 mm×250 mm,5 μm）.The column temperature was fixed on room temperature.Methanol-0.4% phosphoric acid（50∶50） was used as mobile phase,flow-rate was 1.0 mL · min-1.The detecting wavelength was set at 360 nm.Result:The linear range were 0.012 5-0.4 μg（r=0.999 8） and 0.014-0.448 μg（r=0.999 9）.The average recoveries were 97.53% and 100.3%,with the RSD of 1.9% and 1.0%.Conclusion:The method established is rapid and accurate,which could be used for quality control of Silkworm Powder.关键词：Silkworm Powder;quercetin;Kaempferol;RP-HPLC
- 0
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- 105
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- 6
发布时间：2024-09-23 - 摘要：Objective: To establish an method for determination of hydroxysafflor yellow A, gallic acid and aristolochic acid A in Wuwei Zhaxun pill. Method: The hydroxysafflor yellow A, gallic acid and aristolochic acid A in Wuwei Zhaxun pill was determined by HPLC. CAPCELL PAK C18(4.6 mm×250 mm,5 μm)column was used and detection wavelength was at 403, 273, 390 nm. The mobile phase was methanol-0.4% phosphoric acid (30: 70), methanol-0.2%phosphoric acid (7: 93), methanol-0.05% glacial acetic acid (79: 21),with the flow rate of 1.0 mL · min-1.Result: The calibration curves of hydroxysafflor yellow A, gallic acid and aristolochic acid A were in a good linearity over the ranges of 0.116-1.740 μg (r=0.999 97), 0.067-0.673 μg(r=0.999 95), 0.024-0.247 μg(r=0.999 99),respectively. The average recoveries were 98.62%(RSD 2.15%),100.01%(RSD 2.03%),96.83%(RSD 1.74%). Conclusion: The method is simple, accurate and can be used for the control.关键词：hydroxysafflor yellow A;gallic acid;aristolochic acid A;HPLC;Wuwei Zhaxun pill
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for determining puerarin in Chaiqin soft capsules.Method: The HPLC analysis was performed on a C18 column（4.6 mm×250 mm,5 μm） with methanol-water-phosphoric acid（25 ∶ 75 ∶ 0.2） as mobile phase,detected at the wavelength of 250 nm.Result: Puerarinwas linear in the range of 0.559 5-111.9 mg · L-1（r=0.999 8）.The average recovery was 99.85%,RSD 0.83%.the RSDs of precision and reproducibility were 0.52% and 0.57% respectively.Conclusion: The method is simple,accurate and reproducible,which can be used to determine puerarin in Chaiqin soft capsules.关键词：HPLC;Chaiqin soft capsules;puerarin
- 0
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- 75
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- 10
发布时间：2024-09-23 - 摘要：Objective: The content ofquercetin in Ilex kudingcha was determined by HPLC. Method: The separation was performed on a Kromasil C18 column (4.6 mm×250 mm, 5 μm) with methanol-0.4% phosphoric acid (50: 50) as mobile phase, detected at 371 nm and 30℃. Result: Quercetinwas linear in the range of 5.98-47.84 g · mL-1 (r=0.999 7). Conclusion: The content ofquercetin in Ilex kudingcha could be determined by HPLC precisely.关键词：Ilex kudingcha;HPLC;quercetin;content determination
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:Study and revise the quality standard of Zhuangyao Bushen pills.Method:Astragalus membranceus,red ginseng,Dipsacus asper,Radix Astragal,Schisandrae Chiensis Fructus in Zhuangyao Bushen pills were identified by TLC method and the content of asperosaponin Ⅵ was determined by HPLC.Result:TLC identification method for A.membranceus,Angelicae,red ginseng,dipsacus asperoides,Radix Astragal,Schisandrae Sphenantherae Fructus was simple and feasible with strong specificity.AsperosaponinⅥ content was linear in the range of 0.071 6-0.716 g · L-1;the recovery was 99.15%,RSD 1.42%.Conclusion:The method can effectively control the quality of Zhuangyao Bushen pills.关键词：Zhuangyao Bushen pills;TLC;HPLC;quality standards
- 0
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- 99
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- 4
发布时间：2024-09-23 - 摘要：Objective: To establish the method for content determination of Yigangao. Method: HPLC was used to determine the content of icariin. Result: The spots were clear on TLC, and icariin was linear in the range of 0.020 06-0.802 4μg. The average recovery was 100.25%, with RSD of 1.4%. Conclusion: The method established is simple and feasible with good reproducibility.关键词：Yigangao;HPLC;icariin
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a method for determing Salvianolic acid B in Yishen Juanbi capsule.Method: Determination of salvianolic acid B in Yishen Juanbi capsule was performed on Kromasil-C18 column（4.6 mm×250 mm,5 μm）;mobile phase was methanol-acetonitrile-1.7% formic acid solution（28∶8∶64）;flow rate was 1.0 mL · min-1;detection wavelength was set at 286 nm.Result:Salvianolic acid B was linear in the range of 0.150 4-15.04 μg（r=0.999 9）;the average recovery was 98.90%,RSD 1.22%.Conclusion:This method has good repeatability and flexibility.It can be used for quality control in production of Yishen Juanbi capsule.关键词：HPLC;Yishen Juanbi capsule;salvianolic acid B;content determination
- 0
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- 57
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- 2
发布时间：2024-09-23 - 摘要：Objective: To establish evaluation method of quality of borneol in Huoxue Zhitong preparations. Method: A ZB-WAX capillary column was used and the column temperature was kept at 110℃ for simultaneous determination of camphor, iso-borneol and borneol in Huoxue Zhitong Powder and Huoxue Zhitong Capsules. Result: Good linearities were obtained for camphor, iso-borneol and borneol respectively and the average recoveries were all between 95% and 105% with RSD below 3%. Conclusion: The proposed method is accurate, sensitive and simple, which is suitable for quality control of Huoxue Zhitong preparations.关键词：Huoxue Zhitong preparations;borneol;camphor;GC;qualtity control
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish a HPLC method for the determination of eldrin,quertetin and kaempferol in Impatiens balsamina.Method:The determination was performed on a Diamonsil C18 column（4.6 mm×250 mm,5 μm）,with a gredient mobile phase of acetonitrile-0.1% glacial acetic acid;the flow rate was1.0 mL·min-1;column temperature was 30 ℃;the detection wavelength was at 260 nm.Result:The chromatographic peaks of eldrin,quertetin and kaempferol with other components were completely separated.The average recovery was 98.71%,98.96%,98.89%,RSD 2.13%,2.34%,2.38%.Conclusion:The method was rapid and accurate for quality control of I.balsamina,and laid foundations for further research and development.关键词：HPLC;Impatiens balsamina;eldrin;quertetin;kaempferol
- 0
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- 695
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- 22
发布时间：2024-09-23 - 摘要：Objective: To study the chemical constituents of the fruits of Acanthopanax sessiliflorus. Method: The methods of solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography and recrystallization were used for the isolation;spectroscopy was used for the structure identification. Result: Six compounds were isolated and identified as oleanolic acid 3-O-6'-methyl-β-D-glucuronopyranoside (1), 3-O-α-L-arabinopyranosyl-oleanolic acid (2), oleanolic acid 3-O-6'-buthyl-β-D-glucurono-pyranoside (3), momordin lb (4), oleanolic acid (5), 3-O-[(α-L-arabinopyranosyl)-(1→2)]-[-D-glucuronopyranosyl-6-O-methyl ester]-olean-12-ene-28-olic acid (6). Conclusion: Compounds 2, 3 were first isolated from the fruits of A. sessiliflorus.关键词：Acanthopanax sessiliflorus;glycosides of oleanolic acid;structure identification
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish an method for determination of hydroxysafflor yellow A,gallic acid and aristolochic acid A in Wuwei Zhaxun pill.Method:The hydroxysafflor yellow A,gallic acid and aristolochic acid A in Wuwei Zhaxun pill was determined by HPLC.CAPCELL PAK C18（4.6 mm×250 mm,5 μm）column was used and detection wavelength was at 403,273,390 nm.The mobile phase was methanol-0.4% phosphoric acid（30∶70）,methanol-0.2%phosphoric acid（7∶93）,methanol-0.05% glacial acetic acid（79∶21）,with the flow rate of 1.0 mL · min-1.Result:The calibration curves of hydroxysafflor yellow A,gallic acid and aristolochic acid A were in a good linearity over the ranges of 0.116-1.740 μg（r=0.999 97）,0.067-0.673 μg（r=0.999 95）,0.024-0.247 μg（r=0.999 99）,respectively.The average recoveries were 98.62%（RSD 2.15%）,100.01%（RSD 2.03%）,96.83%（RSD 1.74%）.Conclusion:The method is simple,accurate and can be used for the control.关键词：hydroxysafflor yellow A;gallic acid;aristolochic acid A;HPLC;Wuwei Zhaxun pill
- 0
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- 145
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- 6
发布时间：2024-09-23 - 摘要：Objective: Constitutents of stems and leaves of Litsea pungens in Taibai Mountain were studied. Method: Compounds were isolated by silica gel column chromatography, and purified by recrystallization. Their structures were identified by physicochemical properties and spectra analysis. Result: Five chemical compounds were obtained and identified as β-sitosterol (1), palmitic acid (2), pinocembrin (3), apigenin (4), isoquercitrin (5). Conclusion: Compounds 1,2,4 and 5 were isolated from this plant for the first time. Compounds 2, 4 and 5 were isolated from the genus for the first time.关键词：Litsea pungens;chemical constitutents;structure identification
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:The content ofquercetin in Ilex kudingcha was determined by HPLC.Method:The separation was performed on a Kromasil C18 column（4.6 mm×250 mm,5 μm） with methanol-0.4% phosphoric acid（50 ∶ 50） as mobile phase,detected at 371 nm and 30 ℃.Result:Quercetinwas linear in the range of 5.98-47.84 g · mL-1（r=0.999 7）.Conclusion:The content ofquercetin in Ilex kudingcha could be determined by HPLC precisely.关键词：Ilex kudingcha;HPLC;quercetin;content determination
- 0
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- 154
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- 1
发布时间：2024-09-23 - 摘要：Objective: To investigate the chemical constituents of the whole grass of Lespedeza virgat. Method: Various chromatographic techniques were used to separate and purify the chemical constituents from ethyl acetate part and n-butyl alcohol part extract of Lespedeza virgate, and the isolated compounds were identified according to their physicochemical and spectroscopic data. Result: Seven compounds were isolated and identified as physcion (1), chrysophanol (2), kaempferol-3,7-di-O-β-D-glucopy-ranoside (3),kaempferol-3-O-β-D-rutinoside (4), kaempferol-7-O-α-L-rhamnopyranoside (5), n-hexacosanoic acid (6), n-octacosanol (7). Conclusion: The compounds 1,2,3,4,6 were isolated for the first time from this genus, and the compound 7 was isolated from the plant for the first time.关键词：Lespedeza;Lespedeza virgata;chemical constituents
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish the method for content determination of Yigangao.Method:HPLC was used to determine the content of icariin.Result:The spots were clear on TLC,and icariin was linear in the range of 0.020 06-0.802 4μg.The average recovery was 100.25%,with RSD of 1.4%.Conclusion:The method established is simple and feasible with good reproducibility.关键词：Yigangao;HPLC;icariin
- 2
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- 21
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- 1
发布时间：2024-09-23 - 摘要：Objective: Explore safety and effectiveness of medicine in clinical and reasonableness of content limitation to evaluate quality of various Chinese materia medica by berberine hydrochloride as reference substance, and propose an attention to establish quality specification corresponding to the clinical effects. Method: Berberine hydrochloride was determined by HPLC, and he safety and effectiveness in clinical and rationality of quality indexes were discussed by comparing assay and effect in intervention levels of traditional decoction, drugs compatibility and veritaserum. Result: Content of berberine hydrochloride was:(5.52%±0.065%)-(0.12%±0.007%) in the solution extracted traditional decoction, drugs compatibility and veritaserum of Rhizoma Coptidis Chinensis, (3.39%±0.065%)-(0.13%±0.005%) in the solution extracted traditional decoction, drugs compatibility and veritaserum of Cortex Phellodendri Chinensis. Conclusion: Chinese materia medica is the active components alignment, and the reference standard to evaluate Chinese materia medica should be the active components alignment. The content of berberine hydrochloride is lower than the current standard in clinical, and the dose in clinical would lead to effectiveness and safety problem. So should pay attention to the counterparts with the specification of single component in the active components alignment and its clinical effects.
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To establish evaluation method of quality of borneol in Huoxue Zhitong preparations.Method: A ZB-WAX capillary column was used and the column temperature was kept at 110 ℃ for simultaneous determination of camphor,iso-borneol and borneol in Huoxue Zhitong Powder and Huoxue Zhitong Capsules.Result:Good linearities were obtained for camphor,iso-borneol and borneol respectively and the average recoveries were all between 95% and 105% with RSD below 3%.Conclusion:The proposed method is accurate,sensitive and simple,which is suitable for quality control of Huoxue Zhitong preparations.关键词：Huoxue Zhitong preparations;borneol;camphor;GC;qualtity control
- 0
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- 202
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- 14
发布时间：2024-09-23 - 摘要：Objective: To establish FTIR method of brag-zhun. Method: The technique of FTIR and the method of quick comparison were used to determine and compare crude and processed brag-zhun from Yushu. Result: The IR spectra of crude and processed brag-zhun from Yushu were similar, and the absorption area was divided into four regions:OH stretching vibration absorption at 3 436 cm-1, H2O bending vibration absorption at 1 628 cm-1, C-O stretching vibration absorption at 1 425-712 cm-1 and Si-O stretching vibration absorption at 400-550 cm-1. The semblance of brag-zhun with LiI, CaCl2 · 2H2O and tachyhydrite respectively was 89.75%,89.75% and 87.23%. Conclusion: The technique of IR was provided for classification and identification of brag-zhun.关键词：brag-zhun;processed;infrared spectroscopy
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To study the chemical constituents of the fruits of Acanthopanax sessiliflorus.Method:The methods of solvent extraction,silica gel column chromatography,Sephadex LH-20 column chromatography and recrystallization were used for the isolation;spectroscopy was used for the structure identification.Result:Six compounds were isolated and identified as oleanolic acid 3-O-6′-methyl-β-D-glucuronopyranoside（1）,3-O-α-L-arabinopyranosyl-oleanolic acid（2）,oleanolic acid 3-O-6′-buthyl-β-D-glucurono-pyranoside（3）,momordin lb（4）,oleanolic acid（5）,3-O-[（α-L-arabinopyranosyl）-（1→2）]-[-D-glucuronopyranosyl-6-O-methyl ester]-olean-12-ene-28-olic acid（6）.Conclusion:Compounds 2,3 were first isolated from the fruits of A.sessiliflorus.关键词：Acanthopanax sessiliflorus;glycosides of oleanolic acid;structure identification
- 0
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- 148
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- 11
发布时间：2024-09-23 - 摘要：Objective: To investigate the expression levels of Serotonin 3A/B receptor (5-HT3A/BR) in primarily cultured rat hippocampus neurons stimulated with serum respectively from depressive rats and different drugs treated depressive rats for exploring mechanisms of Shuyu Capsule. Method: The rats were divided randomly into five groups including normal control, model with depression, treatment groups treated with Shuyu capsule(0.51 g · kg-1),saikosaponin(0.4 g · kg-1) and fluoxetine(0.002 g · kg-1),respectively. The depression emotion model was set up by the methods of isolated breed and chronic mild stress for 28 days. Serum of rats in every group was collected, inactivated and sterilized. After serum contained hippocampus neurons treatment in every group respectively for 24 hours, Western blot and immunoflurescence were separately used to detect the expression levels of 5-HT3A and 5-HT3B receptor protein in neurons. Result: The expression of 5-HT3A and 5-HT3B receptor in neurons incubating with serum of rats in model group increased obviously (P<0.01,P<0.05)compared with those in the serum from normal control rat treated neurons. Comparing with the neurons treated with model rats serum, the expression of 5-HT3A receptor in neurons incubated with serum of drug treatment rats were significantly improved(P<0.01), but the expression of 5-HT3B receptor was no statistical difference. Conclusion: The expression of 5-HT3 receptor in neurons was closely related with depression, and the differential expression of 5-HT3AR and 5-HT3BR may play important roles in depression emotion. Shuyu, saikosaponin and fluoxetine showed the same tendency on these two proteins expression of neurons, indicating that Shuyu capsule and saikosaponin have notable effect on depression therapy by improving the 5-HT3AR protein expression levels in hippocampus neurons.关键词：hippocampus neuron;Shuyu capsule;5-HT3R
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:Constitutents of stems and leaves of Litsea pungens in Taibai Mountain were studied.Method:Compounds were isolated by silica gel column chromatography,and purified by recrystallization.Their structures were identified by physicochemical properties and spectra analysis.Result:Five chemical compounds were obtained and identified as β-sitosterol（1）,palmitic acid（2）,pinocembrin（3）,apigenin（4）,isoquercitrin（5）.Conclusion:Compounds 1,2,4 and 5 were isolated from this plant for the first time.Compounds 2,4 and 5 were isolated from the genus for the first time关键词：Litsea pungens;chemical constitutents;structure identification
- 0
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- 149
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- 7
发布时间：2024-09-23 - 摘要：Objective: To study on the antioxidant activity of Schisandrae Chinensis Fructus red pigment. Method: With UV-V is spectrophotometry, compared with vitamin C(VC) and vitamin E(VE), the antioxidant activity of Schisandrae Chinensis Fructus red pigment was studied by measuring its scavenging effects on 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH ·),hydroxyl radical(· OH) and superoxide radical(O2-·). Result: Antioxidant activity of Schisandrae Chinensis Fructus red pigment with EC50as index, the results were 27.95 mg · L-1(scavenging DPPH ·), 98.03 mg · L-1(scavenging · OH) and 154.27 mg · L-1(scavenging O2- ·). Its scavenging effects on radicals were not significant with VC, but much stronger than VE. Conclusion: Schisandrae Chinensis Fructus red pigment has strong antioxidant activity and shows a significant dose-effect relationship.关键词：Schisandrae Chinensis Fructus red pigment;radical;antioxidant activity;vitamin C(VC);vitamin E(VE)
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the chemical constituents of the whole grass of Lespedeza virgat.Method:Various chromatographic techniques were used to separate and purify the chemical constituents from ethyl acetate part and n-butyl alcohol part extract of Lespedeza virgate,and the isolated compounds were identified according to their physicochemical and spectroscopic data.Result:Seven compounds were isolated and identified as physcion（1）,chrysophanol（2）,kaempferol-3,7-di-O-β-D-glucopy-ranoside（3）,kaempferol-3-O-β-D-rutinoside（4）,kaempferol-7-O-α-L-rhamnopyranoside（5）,n-hexacosanoic acid（6）,n-octacosanol（7）.Conclusion:The compounds 1,2,3,4,6 were isolated for the first time from this genus,and the compound 7 was isolated from the plant for the first time.关键词：Lespedeza;Lespedeza virgata;chemical constituents
- 0
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- 128
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- 7
发布时间：2024-09-23 - 摘要：Objective: To study the anti-inflammatory mechanism of Qishe pill on stimulated macrophage. Method: Interferon-γ(IFN-γ) plus lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage was used as inflammatory experimental model. Griess reaction for nitric oxide production and Western blot for protein expression examination were performed. Result: Qishe pill inhibited nitric oxide(NO) production in a dose-dependent manner without cytotoxicity. The protein expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX-2) were also suppressed by Qishe pill treatment. Furthermore, phosphorylation of extracellular signal-regulated kinase(ERK), c-Jun NH2-terminal kinase (JNK) and p38 protein was markedly inhibited. Conclusion: Qishe pill suppressed over production of NO and iNOS expression via inhibition of phosphorylation of ERK, JNK and p38/MAPK(mitogen-activated protein kinas) activated signalling pathway partly.关键词：inflammation;nitric oxide;inducible nitric oxide synthase;Qishe pill;molecular mechanism
- 0
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:Explore safety and effectiveness of medicine in clinical and reasonableness of content limitation to evaluate quality of various Chinese materia medica by berberine hydrochloride as reference substance,and propose an attention to establish quality specification corresponding to the clinical effects.Method:Berberine hydrochloride was determined by HPLC,and he safety and effectiveness in clinical and rationality of quality indexes were discussed by comparing assay and effect in intervention levels of traditional decoction,drugs compatibility and veritaserum.Result:Content of berberine hydrochloride was:（5.52%±0.065%）-（0.12%±0.007%） in the solution extracted traditional decoction,drugs compatibility and veritaserum of Rhizoma Coptidis Chinensis,（3.39%±0.065%）-（0.13%±0.005%） in the solution extracted traditional decoction,drugs compatibility and veritaserum of Cortex Phellodendri Chinensis.Conclusion:Chinese materia medica is the active components alignment,and the reference standard to evaluate Chinese materia medica should be the active components alignment.The content of berberine hydrochloride is lower than the current standard in clinical,and the dose in clinical would lead to effectiveness and safety problem.So should pay attention to the counterparts with the specification of single component in the active components alignment and its clinical effects.
- 0
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- 367
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effects of Kaixin Jieyu decoction(KJD) on changes of 5-HT contents and the gene-expression of 5-HT receptor subtypes in the brain of rats with depression. Method: SD rats were randomly divided into 4 groups:control, depression model (CUMS), CUMS+KJD, CUMS+fluoxetine. The depression model of rat was established by chronic unpredictable mild stress (CUMS). The treatments were given contemporarily, KJD group was given crud drug 22 g · kg-1, as fluoxetine was given 2.4 mg · kg-1, and the other groups were given same volume normal saline for once a day for 22 days. Regularly changes on body weight were evaluated and the behaviors changes were recorded after 1, 8, 15, 22 days;22 d later, the cerebral cortex and hippocampus were acquired respectively, the content of monoamines neurotransmitter 5-HT in cerebral cortex was determined by HPLC-ECD and the 5-HT1A/ 2A receptor mRNA were determined by real-time PCR. Result: Compared with control group, the growth of body weight was significantly decreased in those CUMS-induced groups, the consumption of hedonia and number of the horizontal and vertical activity were decreased also (P<0.01,P<0.05), while increased gradually (P<0.05) in the KLD group. The total 5-HT levels in cerebral cortex were declined in CUMS-induced groups, while those in KJD and fluoxetine groups were increased remarkable (P<0.05). In cerebral cortex of the control group, 5-HT1A mRNA level declined while 5-HT2A mRNA level increased, both KJD and fluoxetine showed different decreasing effects. As those in hippocampus, changes were reversed. Conclusion: The depression model of rat can be established by CUMS. These CUMS-induced depression state can be ameliorated by KJD, and possible mechanism consists of improving the level of 5-HT in cerebral cortex and increasing 5-HT1Areceptor mRNA in hippocampus and 5-HT2A receptor mRNA in cerebral cortex, meanwhile reducing 5-HT2A receptor mRNA in hippocampus and 5-HT1A receptor mRNA in cerebral cortex.关键词：Kaixin Jieyu Decoction;depression;5-HT1A/ 2Areceptor;cerebral cortex;hippocampus
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发布时间：2024-09-23 - 摘要：Objective:To establish FTIR method of brag-zhun.Method:The technique of FTIR and the method of quick comparison were used to determine and compare crude and processed brag-zhun from Yushu.Result:The IR spectra of crude and processed brag-zhun from Yushu were similar,and the absorption area was divided into four regions:OH stretching vibration absorption at 3 436 cm-1,H2O bending vibration absorption at 1 628 cm-1,C-O stretching vibration absorption at 1 425-712 cm-1 and Si-O stretching vibration absorption at 400-550 cm-1.The semblance of brag-zhun with LiI,CaCl2 · 2H2O and tachyhydrite respectively was 89.75%,89.75% and 87.23%.Conclusion:The technique of IR was provided for classification and identification of brag-zhun.关键词：brag-zhun;processed;infrared spectroscopy
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- 113
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发布时间：2024-09-23 - 摘要：Objective: To compare the liver-protective effects between traditional decoction and Chinese medicinal dispensing granule of Sini San. Method: Liver injury models were induced by establishing restraint stress and Propionibacterium acnes-lipopolysaccharide (LPS) in mice. Kunming mice were divided into 7 groups,normal control, stress model, positive control, Sini San decoction (600, 1 200 mg · kg-1) and Sini San dispensing granule (600, 1 200 mg · kg-1). Mice were administered for consecutive 6 days and restrained for 18 h. Hepatic function was evaluated by assessing assessing alanine aminotransferase(ALT) and aspartate aminotransferase (AST) level in plasma. Kuming mice were divided into 7 groups, such as normal control, P.acnes-LPS model, positive control, Sini San decoction (600, 1 200 mg · kg-1) and Sini San dispensing granule (600, 1 200 mg · kg-1). Mice were injected with 20 mg · kg-1 P. acnes and 5 μg · kg-1 LPS to induce liver injury. Hepatic function was evaluated by ALT and AST level in plasma. Result: Compared with the model groups, the traditional decoction and Chinese dispensing granule of Sini San significantly reduced ALT and AST levels induced by restraint stress or P.acne-LPS. However, there were no significant difference between the two samples. Conclusion: The traditional decoction and Chinese medicinal dispensing granule of Sini San both are effective on improving the liver function in liver-injury mice model.关键词：Sini San;liver function;restraint stress
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发布时间：2024-09-23 - 摘要：Objective:To investigate the expression levels of Serotonin 3A/B receptor（5-HT3A/BR） in primarily cultured rat hippocampus neurons stimulated with serum respectively from depressive rats and different drugs treated depressive rats for exploring mechanisms of Shuyu Capsule.Method:The rats were divided randomly into five groups including normal control,model with depression,treatment groups treated with Shuyu capsule（0.51 g · kg-1）,saikosaponin（0.4 g · kg-1） and fluoxetine（0.002 g · kg-1）,respectively.The depression emotion model was set up by the methods of isolated breed and chronic mild stress for 28 days.Serum of rats in every group was collected,inactivated and sterilized.After serum contained hippocampus neurons treatment in every group respectively for 24 hours,Western blot and immunoflurescence were separately used to detect the expression levels of 5-HT3A and 5-HT3B receptor protein in neurons.Result:The expression of 5-HT3A and 5-HT3B receptor in neurons incubating with serum of rats in model group increased obviously（P<0.01,P<0.05）compared with those in the serum from normal control rat treated neurons.Comparing with the neurons treated with model rats serum,the expression of 5-HT3A receptor in neurons incubated with serum of drug treatment rats were significantly improved（P<0.01）,but the expression of 5-HT3B receptor was no statistical difference.Conclusion:The expression of 5-HT3 receptor in neurons was closely related with depression,and the differential expression of 5-HT3AR and 5-HT3BR may play important roles in depression emotion.Shuyu,saikosaponin and fluoxetine showed the same tendency on these two proteins expression of neurons,indicating that Shuyu capsule and saikosaponin have notable effect on depression therapy by improving the 5-HT3AR protein expression levels in hippocampus neurons.关键词：hippocampus neuron;Shuyu capsule;5-HT3R
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect and mechanism of alcoholic extract of Pinellia ternate at non-cytotoxic concentration(inhibit rate≤5%)reversing the multidrug resistant (MDR) leukemia cell lines K562/A02. Method: Cells were seeded into 72-well plates at 2×108/L with varying concentrations (2.5,5,10,20,40 μg · L-1) of P. ternate for 72 h, MTT colorimetry was used to determine the cytotoxic effect of alcoholic extract of P. ternate and the sensibility to adriamycin of K562/A02 cell line. Flow cytometry was used to determine the expressive and functional changes of glycoprotein-170(p170)on the cell membrane dealed with alcoholic extract of P. ternate at non-cytotoxic concentration. And daunorubicin concentration in the cells was detected by flow cytometry. The statistical treatment with SPSS 16.0 was applied to deal with all the experimental data. Result: Alcoholic extract of P. ternate had definite cytotoxic effect on K562/A02,the non-cytotoxic dose of oxymatrine was 6.5 μg · L-1. Non-cytotoxic dose of alcoholic extract of P. ternate can significantly decrease the medium inhibition concentration (IC50) value of K562/ADM cell line to adriamycin (ADM).The IC50was obviously reduced from (30.9±0.11) μg · L-1 to (10.1±0.21) μg · L-1,alcoholic extract of P. ternate at non-cytotoxic concentration could partly reverse MDR of K562/A02 (3.06times), and could descend the expression of p170 from 91.21% to 45.12%(P<0.01).The exosmosis test of daunorubicin showed that intracellular concentration of chemotherapeutics concentration increased evidently. Conclusion: Alcoholic extract of P. ternate is able to partly reverse MDR of K562/A02 cell line could descend the expression of P170,and can restrain the function of pumping chemotherapeutics out of the cel1 and enhance the efficient intracellular concentration of chemotherapeutics which can kill leukemia cells of multidrug resistance,and partly reversed MDR of K562/A02.关键词：alcoholic extract of Pinellia ternate;K562/A02 cell line;multidrug resistance
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发布时间：2024-09-23 - 摘要：Objective:To study on the antioxidant activity of Schisandrae Chinensis Fructus red pigment.Method:With UV-V is spectrophotometry,compared with vitamin C（VC） and vitamin E（VE）,the antioxidant activity of Schisandrae Chinensis Fructus red pigment was studied by measuring its scavenging effects on 1,1-Diphenyl-2-picrylhydrazyl radical（DPPH ·）,hydroxyl radical（· OH） and superoxide radical（O-2 ·）.Result:Antioxidant activity of Schisandrae Chinensis Fructus red pigment with EC50as index,the results were 27.95 mg · L-1（scavenging DPPH ·）,98.03 mg · L-1（scavenging · OH） and 154.27 mg · L-1（scavenging O-2 ·）.Its scavenging effects on radicals were not significant with VC,but much stronger than VE.Conclusion:Schisandrae Chinensis Fructus red pigment has strong antioxidant activity and shows a significant dose-effect relationship.关键词：Schisandrae Chinensis Fructus red pigment;radical;antioxidant activity;vitamin C（VC）;vitamin E（VE）
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- 208
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发布时间：2024-09-23 - 摘要：Objective: To investigate the effect of wogonin on the nuclear translocation and expression of nuclear factor-kappa B(NF-κB) in NR8383 cells infected by influenza virus and the relationship with Toll like receptor 7(TLR7) mediated myeloid differentiation factor 88(MyD88)-dependent signaling pathway. Method: After 1 h adsorption of influenza virus, the medium containing 0.016 g · L-1 wogonin was added to NR8383 cells in wells. At 24 h after drug application, reverse transcription polymerase chain reaction(RT-PCR) was used to detect the mRNA level of MyD88, NF-κB p65 and TLR7. At 4, 6, 24 h after drug application, immunocytochemistry was used to detect nuclear translocation of NF-κB p65 in the cells and perform semi-quantity analysis. At 24 h after wogonin application, Western blot was used to detect the expression of NF-κB p65 protein. Result: Wogonin reduced the mRNA level of TLR7, MyD88, and NF-κB p65(P<0.05,P<0.01,P<0.05)and inhibited the nuclear translocation and expression of NF-κB p65(P<0.01). Conclusion: Wogonin inhibited the nuclear translocation and expression of NF-κB by repressing TLR7 mediated MyD88-dependent signaling pathway, and alleviated the inflammatory reaction in influenza virus infection.关键词：wogonin;influenza virus;TLR7;MyD88;NF-κB p65
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发布时间：2024-09-23 - 摘要：Objective:To study the anti-inflammatory mechanism of Qishe pill on stimulated macrophage.Method:Interferon-γ（IFN-γ） plus lipopolysaccharide（LPS） stimulated RAW 264.7 macrophage was used as inflammatory experimental model.Griess reaction for nitric oxide production and Western blot for protein expression examination were performed.Result:Qishe pill inhibited nitric oxide（NO） production in a dose-dependent manner without cytotoxicity.The protein expression of inducible nitric oxide synthase（iNOS） and cyclooxygenase（COX-2） were also suppressed by Qishe pill treatment.Furthermore,phosphorylation of extracellular signal-regulated kinase（ERK）,c-Jun NH2-terminal kinase（JNK） and p38 protein was markedly inhibited.Conclusion:Qishe pill suppressed over production of NO and iNOS expression via inhibition of phosphorylation of ERK,JNK and p38/MAPK（mitogen-activated protein kinas） activated signalling pathway partly.关键词：inflammation;nitric oxide;inducible nitric oxide synthase;Qishe pill;molecular mechanism
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- 155
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发布时间：2024-09-23 - 摘要：Objective: To study on the effect of Siraitiae Fructus extracts from different growth period fruit on postprandial blood glucose levels in mice, and examine the impact on postprandial insulin and α-glucosidase enzyme activity. Method: Kuenming mice were divided into starch load (6 g · kg-1) and glucose load (2 g · kg-1) group, each group consists of 9 subgroups:normal control, starch load or glucose load control, positive control (acarbose 0.01 g · kg-1), 90 days Siraitiae Fructus extracts high, medium and low dose (1,0.5,0.25 g · kg-1), 60 days Siraitiae Fructus extracts high, medium and low dose (1,0.5,0.25 g · kg-1). The postprandial blood glucose induced by starch and glucose were observed after 7 days Siraitiae Fructus extracts administration, and the postprandial insulin levels were determined via ELISA method. Moreover, the inhibition effect of Siraitiae Fructus extracts on α-glucosidase activity was determined in vitro. Result: All dosage of 90 days and 60 days Siraitiae Fructus extracts inhibited peak value of postprandial blood glucose;all dosage of 90 days Siraitiae Fructus extracts reduced glycemic index induced by starch (P<0.05), and the glycemic index induced by glucose was reduced at 0.25 g · kg-1(P<0.05);moreover, 0.5 g · kg-1of 60 days Siraitiae Fructus extracts significantly reduced glycemic index induced by starch (P<0.01), but all dose of 60 days Siraitiae Fructus extracts only demonstrated a trend to reduce the glycemic index induced by glucose. All dose of 90 days Siraitiae Fructus extracts significantly increased postprandial insulin (P<0.001), and 60 days Siraitiae Fructus extracts increased postprandial insulin dose dependently. The IC50 value of 90 and 60 days Siraitiae Fructus extracts was 11.51 g · L-1 and 2.40 g · L-1, respectively. Conclusion: Mangosteen extract is effectual in the inhibition of the rapid increase of postprandial blood glucose, which are primarily mediated by increasing postprandial insulin levels andα-glucosidase inhibition, there is a difference in hypoglycemic mechanism between Siraitiae Fructus extracts from different growth period fruit.关键词：Siraitiae Fructus;blood glucose;insulin;α-glucosidase
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effects of Kaixin Jieyu decoction（KJD） on changes of 5-HT contents and the gene-expression of 5-HT receptor subtypes in the brain of rats with depression.Method:SD rats were randomly divided into 4 groups:control,depression model（CUMS）,CUMS+KJD,CUMS+fluoxetine.The depression model of rat was established by chronic unpredictable mild stress（CUMS）.The treatments were given contemporarily,KJD group was given crud drug 22 g · kg-1,as fluoxetine was given 2.4 mg · kg-1,and the other groups were given same volume normal saline for once a day for 22 days.Regularly changes on body weight were evaluated and the behaviors changes were recorded after 1,8,15,22 days;22 d later,the cerebral cortex and hippocampus were acquired respectively,the content of monoamines neurotransmitter 5-HT in cerebral cortex was determined by HPLC-ECD and the 5-HT1A/ 2A receptor mRNA were determined by real-time PCR.Result:Compared with control group,the growth of body weight was significantly decreased in those CUMS-induced groups,the consumption of hedonia and number of the horizontal and vertical activity were decreased also（P<0.01,P<0.05）,while increased gradually（P<0.05） in the KLD group.The total 5-HT levels in cerebral cortex were declined in CUMS-induced groups,while those in KJD and fluoxetine groups were increased remarkable（P<0.05）.In cerebral cortex of the control group,5-HT1A mRNA level declined while 5-HT2A mRNA level increased,both KJD and fluoxetine showed different decreasing effects.As those in hippocampus,changes were reversed.Conclusion:The depression model of rat can be established by CUMS.These CUMS-induced depression state can be ameliorated by KJD,and possible mechanism consists of improving the level of 5-HT in cerebral cortex and increasing 5-HT1Areceptor mRNA in hippocampus and 5-HT2A receptor mRNA in cerebral cortex,meanwhile reducing 5-HT2A receptor mRNA in hippocampus and 5-HT1A receptor mRNA in cerebral cortex.关键词：Kaixin Jieyu Decoction;depression;5-HT1A/ 2Areceptor;cerebral cortex;hippocampus
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- 465
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of tumor necrosis factor α(TNF-α) on glucose consumption in 3T3-L1 adipocyte and find the method of establishing a cell insulin resistance model. Method: The 3T3-L1 preadipocytes were differentiated to 3T3-L1 adipocytes with 1-methyl-3-isobuthylxanthine, dexamethasone and insulin. 3T3-L1 adipocytes were exposed to 20, 10, 5 μg · L-1 TNF-α for 96 h, then added 100 nmol · L-1 insulin for 30 min. The amount of glucose consumption was determined by detecting the glucose content in cell culture supernatants with glucose oxidase(GOD) assay. Result: TNF-α markedly decreased both basal and insulin-stimulated glucose uptake.This effect was concentration-dependent, and 20 μg · L-1TNF-α decreased glucose uptake of 79.2% and 81.4% (P<0.05) in the basal and 100 μg · L-1 insulin-stimulated conditions, respectively. Conclusion: 20 μg · L-1TNF-α can induce insulin resistance in 3T3-L1 adipocytes.A novel and reliable cell model of insulin resistance is successfully established.关键词：TNF-α;insulin resistance;insulin;3T3-L1 cells
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发布时间：2024-09-23 - 摘要：Objective:To compare the liver-protective effects between traditional decoction and Chinese medicinal dispensing granule of Sini San.Method:Liver injury models were induced by establishing restraint stress and Propionibacterium acnes-lipopolysaccharide（LPS） in mice.Kunming mice were divided into 7 groups,normal control,stress model,positive control,Sini San decoction（600,1 200 mg · kg-1） and Sini San dispensing granule（600,1 200 mg · kg-1）.Mice were administered for consecutive 6 days and restrained for 18 h.Hepatic function was evaluated by assessing assessing alanine aminotransferase（ALT） and aspartate aminotransferase（AST） level in plasma.Kuming mice were divided into 7 groups,such as normal control,P.acnes-LPS model,positive control,Sini San decoction（600,1 200 mg · kg-1） and Sini San dispensing granule（600,1 200 mg · kg-1）.Mice were injected with 20 mg · kg-1 P.acnes and 5 μg · kg-1 LPS to induce liver injury.Hepatic function was evaluated by ALT and AST level in plasma.Result:Compared with the model groups,the traditional decoction and Chinese dispensing granule of Sini San significantly reduced ALT and AST levels induced by restraint stress or P.acne-LPS.However,there were no significant difference between the two samples.Conclusion:The traditional decoction and Chinese medicinal dispensing granule of Sini San both are effective on improving the liver function in liver-injury mice model.关键词：Sini San;liver function;restraint stress
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- 313
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发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of Gegen decoction (GGD) and the potential mechanism on primary dysmenorrhea with cold-damp coagulation induced byoxytocin and cold simulating. Method: Seventy female ICR mice were randomly divided into seven groups:normal group, model group, buprofen group, Guizhi Fuling capsule group, low-dose GGD group, middle-dose GGD group and high-dose GGD group. Subcutaneous injection of estrogen and cold stimulation were used for 3 consecutive days, and peritoneal injection of oxytocin on the 4th day to construct primary dysmenorrhea model with cold-damp coagulation, the writhing response and the levels of nitric oxide (NO) and calcium ion(Ca2+) in mice uterine tissue were investigated. Meanwhile, ibuprofen group received ibuprofen (0.12 g · kg-1), GFC group received Guizhi Fuling Capsule (1.12 g · kg-1), GGD group received GGD (low-dose, 3.5 g · kg-1;middle-dose, 7.0 g · kg-1;high-dose, 14.0 g · kg-1). Result: GGD could inhibit the writhing response effectively (the inhibition rate was 81.03%, 84.14%, 90.15% at low, middle and high-dose, respectively) and increase NO levels and reduce Ca2+ concentration in uterine tissue of mice (P<0.001). The efficacy of GGD was equal to the positive drug ibuprofen and Guizhi Fuling capsule. Conclusion: GGD could effectively alleviate the spasm of uterine smooth induced by oxytocin and cold. The mechanism might be relative to regulation of uterine NO and Ca2+ levels.关键词：Gegen decoction;primary dysmenorrhea;cold-damp coagulation
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect and mechanism of alcoholic extract of Pinellia ternate at non-cytotoxic concentration（inhibit rate≤5%）reversing the multidrug resistant（MDR） leukemia cell lines K562/A02.Method:Cells were seeded into 72-well plates at 2×108/L with varying concentrations（2.5,5,10,20,40 μg · L-1） of P.ternate for 72 h,MTT colorimetry was used to determine the cytotoxic effect of alcoholic extract of P.ternate and the sensibility to adriamycin of K562/A02 cell line.Flow cytometry was used to determine the expressive and functional changes of glycoprotein-170（p170）on the cell membrane dealed with alcoholic extract of P.ternate at non-cytotoxic concentration.And daunorubicin concentration in the cells was detected by flow cytometry.The statistical treatment with SPSS 16.0 was applied to deal with all the experimental data.Result:Alcoholic extract of P.ternate had definite cytotoxic effect on K562/A02,the non-cytotoxic dose of oxymatrine was 6.5 μg · L-1.Non-cytotoxic dose of alcoholic extract of P.ternate can significantly decrease the medium inhibition concentration（IC50） value of K562/ADM cell line to adriamycin（ADM）.The IC50was obviously reduced from（30.9±0.11） μg · L-1 to（10.1±0.21） μg · L-1,alcoholic extract of P.ternate at non-cytotoxic concentration could partly reverse MDR of K562/A02（3.06times）,and could descend the expression of p170 from 91.21% to 45.12%（P<0.01）.The exosmosis test of daunorubicin showed that intracellular concentration of chemotherapeutics concentration increased evidently.Conclusion:Alcoholic extract of P.ternate is able to partly reverse MDR of K562/A02 cell line could descend the expression of P170,and can restrain the function of pumping chemotherapeutics out of the cel1 and enhance the efficient intracellular concentration of chemotherapeutics which can kill leukemia cells of multidrug resistance,and partly reversed MDR of K562/A02.关键词：alcoholic extract of Pinellia ternate;K562/A02 cell line;multidrug resistance
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发布时间：2024-09-23 - 摘要：Objective: Using rat intravenous endotoxin model of fever to observe the antifebrile effect of Maxing Shigan Tang. Method: the effects of Maxing Shigan Tang on body temperature was observed on the fever induced by intravenous injection of lipopolysaccharide(LPS) at the dose 5,10,20, 40, 60, 80 μg · kg-1,respectively. The rats were randomly divided into blank group, model group, treatment group (low, medium and high dose respectively 1.33, 4.5, 15.2 g · kg-1), the model was induced immediately after administration. Result: Intravenous injection of endotoxin could increase body temperature significantly (P<0.01). The results showed that the dose of intravenous injection of endotoxin induced SD rat fever model was 20 μg · kg-1. The body temperature was significantly lower compared with model group after fed with low,medium and high dose of Maxing Shigan Tang (P<0.05). TRI showed a clear dose-response relationship. Conclusion: Maxing Shigan Tang has a better antifebrile effects on the fever induced by intravenous injection of LPS in rats, and show a dose-dependent manner.关键词：LPS;fever;TRI;Maxing Shigan tang;antifebrile effects
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发布时间：2024-09-23 - 摘要：Objective:To investigate the effect of wogonin on the nuclear translocation and expression of nuclear factor-kappa B（NF-κB） in NR8383 cells infected by influenza virus and the relationship with Toll like receptor 7（TLR7） mediated myeloid differentiation factor 88（MyD88）-dependent signaling pathway.Method:After 1 h adsorption of influenza virus,the medium containing 0.016 g · L-1 wogonin was added to NR8383 cells in wells.At 24 h after drug application,reverse transcription polymerase chain reaction（RT-PCR） was used to detect the mRNA level of MyD88,NF-κB p65 and TLR7.At 4,6,24 h after drug application,immunocytochemistry was used to detect nuclear translocation of NF-κB p65 in the cells and perform semi-quantity analysis.At 24 h after wogonin application,Western blot was used to detect the expression of NF-κB p65 protein.Result:Wogonin reduced the mRNA level of TLR7,MyD88,and NF-κB p65（P<0.05,P<0.01,P<0.05）and inhibited the nuclear translocation and expression of NF-κB p65（P<0.01）.Conclusion:Wogonin inhibited the nuclear translocation and expression of NF-κB by repressing TLR7 mediated MyD88-dependent signaling pathway,and alleviated the inflammatory reaction in influenza virus infection.关键词：wogonin;influenza virus;TLR7;MyD88;NF-κB p65
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- 435
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发布时间：2024-09-23 - 摘要：Objective: To observe the effects of Zhiyuanfang inhibiting platelets amplified inflammation and proliferation of synovial cells in the rheumatoid arthritis (RA) rats. Method: Normal cultured RSC364 cells were divided into normal control group, RA model group, activated platelet intervention group and Zhiyuanfang group.The different concentrations of tumor necrosis factor-α(TNF-α) were used to induce RA rat cell models, stimulate platelets activating by bovine type II collagen, and interfere platelet intervention group with Zhiyuanfang containing serum. The expression of interleukin-6(IL-6),interleukin-8(IL-8) and P-selecting were assayed respectively by enzyme-linked immunosorbnent assay (ELISA), and the proliferation of RSC364 cells were determined by MTT colorimetric assay. Result: ①The expression of P-selecting was at peak when platelets were activated by bovine type II collagen for 5 h.②the expression of IL-6 and IL-8 on synovial cells in RA model group was increased, which was compared with the same concentration of normal group showed a significant difference(P<0.05);the rate of RSC364 cells proliferation was also higher than normal group(P<0.05)in a dose-dependent manner.③Compared with RA model group, the expression of IL-6 and IL-8 on synovial cells in platelets intervention group was significantly increased(P<0.05, P<0.01), and the rate of synovial cells proliferation was also higher(P<0.05).④ Compared with platelets intervention group, the expression of IL-6 and IL-8 on synovial cells in Zhiyuanfang group was significantly reduced (P<0.05,P<0.01), the proliferation rate of synovial cells was declined(P<0.05), and the expression of P-selecting was also lower. Conclusion: Activated platelets amplify the inflammation and proliferation of synoviocytes;Zhiyuanfang effectively can relieve the amplification of platelets on the inflammation and proliferation in RA rats probably by the inhibition of P-selecting expression.关键词：rheumatoid arthritis (RA);activated platelet;Zhiyuanfang;IL-6;IL-8;P-selecting
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发布时间：2024-09-23 - 摘要：Objective:To study on the effect of Siraitiae Fructus extracts from different growth period fruit on postprandial blood glucose levels in mice,and examine the impact on postprandial insulin and α-glucosidase enzyme activity.Method:Kuenming mice were divided into starch load（6 g · kg-1） and glucose load（2 g · kg-1） group,each group consists of 9 subgroups:normal control,starch load or glucose load control,positive control（acarbose 0.01 g · kg-1）,90 days Siraitiae Fructus extracts high,medium and low dose（1,0.5,0.25 g · kg-1）,60 days Siraitiae Fructus extracts high,medium and low dose（1,0.5,0.25 g · kg-1）.The postprandial blood glucose induced by starch and glucose were observed after 7 days Siraitiae Fructus extracts administration,and the postprandial insulin levels were determined via ELISA method.Moreover,the inhibition effect of Siraitiae Fructus extracts on α-glucosidase activity was determined in vitro.Result:All dosage of 90 days and 60 days Siraitiae Fructus extracts inhibited peak value of postprandial blood glucose;all dosage of 90 days Siraitiae Fructus extracts reduced glycemic index induced by starch（P<0.05）,and the glycemic index induced by glucose was reduced at 0.25 g · kg-1（P<0.05）;moreover,0.5 g · kg-1of 60 days Siraitiae Fructus extracts significantly reduced glycemic index induced by starch（P<0.01）,but all dose of 60 days Siraitiae Fructus extracts only demonstrated a trend to reduce the glycemic index induced by glucose.All dose of 90 days Siraitiae Fructus extracts significantly increased postprandial insulin（P<0.001）,and 60 days Siraitiae Fructus extracts increased postprandial insulin dose dependently.The IC50 value of 90 and 60 days Siraitiae Fructus extracts was 11.51 g · L-1 and 2.40 g · L-1,respectively.Conclusion:Mangosteen extract is effectual in the inhibition of the rapid increase of postprandial blood glucose,which are primarily mediated by increasing postprandial insulin levels andα-glucosidase inhibition,there is a difference in hypoglycemic mechanism between Siraitiae Fructus extracts from different growth period fruit.关键词：Siraitiae Fructus;blood glucose;insulin;α-glucosidase
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- 404
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of effective parts from Musa basjoo(EPM) on blood glucose and glucose tolerance in mice. Method: Diabetic model was induced by intraperitoneal injection of Alloxan once. Diabetic mice were randomly divided into model group, Metformin group and high,middle and low dose of EPM groups(200,100,50 mg · kg-1). All groups were fed for 14 consecutive days, and then the blood glucose was detected on the fifteenth day. Effect on the glucose tolerance in model and normal mice were detected at the same time. Result: EPM could significantly decrease the blood glucose level of diabetic mice(P<0.05)and ameliorate the glucose tolerance in normal and diabetic mice(P<0.05), but it had no significant effect on the blood glucose level of normal mice. Conclusion: EPM can significantly decrease the blood glucose level in diabetic mice and ameliorate the glucose tolerance in normal and diabetic mice.关键词：Musa basjoo;effective parts;blood glucose;glucose tolerance
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of tumor necrosis factor α（TNF-α） on glucose consumption in 3T3-L1 adipocyte and find the method of establishing a cell insulin resistance model.Method:The 3T3-L1 preadipocytes were differentiated to 3T3-L1 adipocytes with 1-methyl-3-isobuthylxanthine,dexamethasone and insulin.3T3-L1 adipocytes were exposed to 20,10,5 μg · L-1 TNF-α for 96 h,then added 100 nmol · L-1 insulin for 30 min.The amount of glucose consumption was determined by detecting the glucose content in cell culture supernatants with glucose oxidase（GOD） assay.Result: TNF-α markedly decreased both basal and insulin-stimulated glucose uptake.This effect was concentration-dependent,and 20 μg · L-1TNF-α decreased glucose uptake of 79.2% and 81.4%（P<0.05） in the basal and 100 μg · L-1 insulin-stimulated conditions,respectively.Conclusion:20 μg · L-1TNF-α can induce insulin resistance in 3T3-L1 adipocytes.A novel and reliable cell model of insulin resistance is successfully established.关键词：TNF-α;insulin resistance;insulin;3T3-L1 cells
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发布时间：2024-09-23 - 摘要：Objective: To observe protective effects of Astragalus injection on renal function, morphological changes in type 2 diabetic KKAy mice. Method: Male KKAy mice were randomly divided into model group, Astragalus injection treatment group(ip, 3 mL ·kg-1 ·d-1), the same age male C57BL/6J mice were selected as normal control group. Blood glucose regularly, blood urea and serum creatinine at 20,24 and 28 weeks of age were measured respectively. Light microscopy and electron microscope were used to observ renal pathological changes in mice of different ages. Result: At 20 weeks of age,blood glucose and blood urea levels in model group KKAy mice were significantly higher than the normal mice (P<0.01). At 24 and 28 weeks of age, serum creatinine levels were significantly higher than the normal mice (P<0.01). At Astragalus injection treatment group KKAy mice with 20 weeks of age, blood glucose was significantly higher than that of normal group(P<0.01), but lower than the model group mice(P<0.05 or P<0.01). At 24 and 28 weeks of age, Astragalus injection treatment group KKAy mice serum creatinine level was significantly lower than model group mice (P <0.01); and from 20 weeks of age, blood urea levels were significantly lower than the model group mice (P <0.01), but compared with normal group, no significant difference was found. Model group KKAy mice showed widened mesangial areas, basement membrane thickening, vacuoles in renal tubular epithelial cells, increased renal interstitial connective tissue and other pathological changes from 20 weeks of age, lesions with ages increased. Lesions in the mice treated by Astragalus injection were markedly improved. Conclusion: Astragalus injection can improve kidney function, reduced pathological changes in kidney of KKAy mice with type 2 diabetes.关键词：KKAy mice;Astragalus injection;diabetic nephropathy;pathological changes
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发布时间：2024-09-23 - 摘要：Objective:To investigate the influence of Gegen decoction（GGD） and the potential mechanism on primary dysmenorrhea with cold-damp coagulation induced byoxytocin and cold simulating.Method:Seventy female ICR mice were randomly divided into seven groups:normal group,model group,buprofen group,Guizhi Fuling capsule group,low-dose GGD group,middle-dose GGD group and high-dose GGD group.Subcutaneous injection of estrogen and cold stimulation were used for 3 consecutive days,and peritoneal injection of oxytocin on the 4th day to construct primary dysmenorrhea model with cold-damp coagulation,the writhing response and the levels of nitric oxide（NO） and calcium ion（Ca2+） in mice uterine tissue were investigated.Meanwhile,ibuprofen group received ibuprofen（0.12 g · kg-1）,GFC group received Guizhi Fuling Capsule（1.12 g · kg-1）,GGD group received GGD（low-dose,3.5 g · kg-1;middle-dose,7.0 g · kg-1;high-dose,14.0 g · kg-1）.Result:GGD could inhibit the writhing response effectively（the inhibition rate was 81.03%,84.14%,90.15% at low,middle and high-dose,respectively） and increase NO levels and reduce Ca2+ concentration in uterine tissue of mice（P<0.001）.The efficacy of GGD was equal to the positive drug ibuprofen and Guizhi Fuling capsule.Conclusion:GGD could effectively alleviate the spasm of uterine smooth induced by oxytocin and cold.The mechanism might be relative to regulation of uterine NO and Ca2+ levels.关键词：Gegen decoction;primary dysmenorrhea;cold-damp coagulation
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发布时间：2024-09-23 - 摘要：Objective: To investigate the protective effects and its mechanism of Supplemental Sini San(SNS) on hepatic fibrosis in rats. Method: The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum. Eighty Wister rats were divided into eight groups:normal control,model control,Sini San Jiawei(SNSJW) high-dose,mediate-dose and low-dose,prevention and SNS group. Except the normal control groups,all the other groups were injected with pig's serum without inactivation into abdominal cavity, twice per week and 0.5 mL each time,persisting for 10 weeks. The prevention groups were orally given with the medicines(SNSJW 7 g · kg-1,10 weeks)at the same time models wasduplicated, the other treatment groups were dealed with just as the prevention groups 6 weeks later, lasted for 4 weeks. The dose was following:SNS group 4 g · kg-1, SNSJW high-dose group 14 g · kg-1, mediate-dose group 7 g · kg-1,low-dose group 3.5 g · kg-1. The changes of hepatic fibrosis were detected. The content of liver tissue interleukin-1(IL-1) and tumor necrosis factor-alpha (TNF-α)were tested by RIA. In situ hybridization assay and immunohistochemical S-P method were used to detect the expression of transforming growth factor-β1(TGF-β1) mRNA and α-smoth muscle actin(α-SMA) mRNA and there protein in hepatic fibrosis tissues. Result: Compared with model group in SNSJW mediate-dose group, the content of HYP, IL-1,TNF-α in liver tissue was depressed significantly, the expression of TGF-β1 and α-SMA genes was significantly lower in SNSJW mediate-dose group. Conclusion: Supplemental SNS has an action on hepatic fibrosis in rats. The mechanism is related with its inhibiting the expression of TGF-β1 and α-SMA genes in rats liver.关键词：Sini San;liver fibrosis;transforming growth factor-β1;α-smooth muscle actin
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发布时间：2024-09-23 - 摘要：Objective:Using rat intravenous endotoxin model of fever to observe the antifebrile effect of Maxing Shigan Tang.Method: the effects of Maxing Shigan Tang on body temperature was observed on the fever induced by intravenous injection of lipopolysaccharide（LPS） at the dose 5,10,20,40,60,80 μg · kg-1,respectively.The rats were randomly divided into blank group,model group,treatment group（low,medium and high dose respectively 1.33,4.5,15.2 g · kg-1）,the model was induced immediately after administration.Result: Intravenous injection of endotoxin could increase body temperature significantly（P<0.01）.The results showed that the dose of intravenous injection of endotoxin induced SD rat fever model was 20 μg · kg-1.The body temperature was significantly lower compared with model group after fed with low,medium and high dose of Maxing Shigan Tang（P<0.05）.TRI showed a clear dose-response relationship.Conclusion: Maxing Shigan Tang has a better antifebrile effects on the fever induced by intravenous injection of LPS in rats,and show a dose-dependent manner.关键词：LPS;fever;TRI;Maxing Shigan tang;antifebrile effects
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- 551
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发布时间：2024-09-23 - 摘要：Objective: The aim of our study is to investigate the effect of Yulangsan flavonoids(YLSF) against CCl4-induced liver fibrosis in rats and study its mechanism. Method: The SD rats were divided into two groups randomly: hepatic fibrosis group and normal control group (NC). The rats of hepatic fibrosis group were induced by intragastric administration (ig) of 50% CCl4, and rats in NC group were given normal saline (NS). The rats of hepatic fibrosis group confirmed by the pathological inspection were divided into 2 groups randomly:Drug intervention group and the model group which were treated with NS. The drug intervention group was divided randomly into 3 different does YLSF groups (20, 40,80 mg · kg-1) and a positive control group (colchicine tablets 0.20 mg · kg-1). All rats were treated with drugs or NS for four consecutive weeks ig, one time a day. Twenty-four hours after the last administration of drugs, all rats were sacrificed, and the blood serum and hepatic tissue were taken quickly. The activities of alanine transaminase(ALT) and aspartale transaminase (AST) in the serum and the levels of superoxide dismutase(SOD), malonaldehyde(MDA), glutathione(GSH), and glutathione synthesis (GSH-Px) in hepatic tissue were analyzed, and the degree of hepatic injury was examined. Result: The content of AST, ALT in serum of each YLSF groups’ rats were (207.85±101.72),(131.55±35.09), (129.98±37.21) U · L-1, (90.51±44.24), (47.79±16.11), (44.56±16.31) U · L-1;and the content of SOD, MDA, GSH-Px, GSH in the tissue of each YLSF groups’ rats were (143.14±42.82), (146.53±31.98), (147.41±32.82) U · mg-1, (2.57±0.54), (2.19±0.57), (2.11±0.59) nmol · mg-1, (463.55±271.07), (659.14±162.23), (752.08±200.70) nmol · mg-1, (5.06±1.09), (6.10±0.97), (6.89±0.98) nmol · mg-1. Compared with the model control group, the increased activity of AST in serum and the increased content of MDA in liver induced by CCl4 were decreased significantly, and the decreased levels of SOD in liver was increased significantly in all dose of YLSF groups and positive control group. The activity of ALT in serum was decreased significantly (P<0.01) and the content of GSH (P<0.01) in liver was increased in the high dose and the middle dose of YLSF groups and positive control group. Meanwhile the activity of GSH-Px in the liver was increased significantly in the high dose and the middle dose of YLSF groups (P<0.01). The degree of hepatic injury in all dose of YLSF groups and positive control group could be lessened (P<0.05 or P<0.01). Conclusion: Our findings demonstrate that YLSF has a certain curative effect on CCl4-induced liver fibrosis in rats, and its mechanism maybe involve in attenuating free radical and inhibiting the lipid peroxidation.关键词：Yulangsan;flavonoids;liver fibrosis
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发布时间：2024-09-23 - 摘要：Objective:To observe the effects of Zhiyuanfang inhibiting platelets amplified inflammation and proliferation of synovial cells in the rheumatoid arthritis（RA） rats.Method:Normal cultured RSC364 cells were divided into normal control group,RA model group,activated platelet intervention group and Zhiyuanfang group.The different concentrations of tumor necrosis factor-α（TNF-α） were used to induce RA rat cell models,stimulate platelets activating by bovine type II collagen,and interfere platelet intervention group with Zhiyuanfang containing serum.The expression of interleukin-6（IL-6）,interleukin-8（IL-8） and P-selecting were assayed respectively by enzyme-linked immunosorbnent assay（ELISA）,and the proliferation of RSC364 cells were determined by MTT colorimetric assay.Result:①The expression of P-selecting was at peak when platelets were activated by bovine type II collagen for 5 h.②the expression of IL-6 and IL-8 on synovial cells in RA model group was increased,which was compared with the same concentration of normal group showed a significant difference（P<0.05）;the rate of RSC364 cells proliferation was also higher than normal group（P<0.05）in a dose-dependent manner.③Compared with RA model group,the expression of IL-6 and IL-8 on synovial cells in platelets intervention group was significantly increased（P<0.05,P<0.01）,and the rate of synovial cells proliferation was also higher（P<0.05）.④ Compared with platelets intervention group,the expression of IL-6 and IL-8 on synovial cells in Zhiyuanfang group was significantly reduced（P<0.05,P<0.01）,the proliferation rate of synovial cells was declined（P<0.05）,and the expression of P-selecting was also lower.Conclusion:Activated platelets amplify the inflammation and proliferation of synoviocytes;Zhiyuanfang effectively can relieve the amplification of platelets on the inflammation and proliferation in RA rats probably by the inhibition of P-selecting expression.关键词：rheumatoid arthritis（RA）;activated platelet;Zhiyuanfang;IL-6;IL-8;P-selecting
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发布时间：2024-09-23 - 摘要：Objective: To study the effect of dihydroartemisinin on immune-regulation of T-lymphocytes from peripheral blood in adjuvant-induced arthritis (AA) rats. Method: Sprague Dawley rats with a mean weight of 160-180 g were randomly divided into 6 groups, including normal group, AA model group, methotrexate group(1 mg · kg-1),dihydroartemisinine high-dose, medium-dose and low-dose group(11.2,5.6,2.8 mg ·kg-1 ). The rat adjuvant arthritis model were induced. The drugs were administrated by ig for consecutive 28 days after inducing AA model. T-lymphocyte in peripheral blood was detected by flow cytometry,and the levels of interferon-γ(INF-γ) and interleukin-4(IL-4) in serum were measured by ELISA on AA animal model treated bydihydroartemisinin. Result: Compared with the normal group, the ratio of CD4+T, CD4+T/ CD8+T from peripheral blood in model group and IFN-γ levels in serum were significantly increased(P<0.01),and the levels of IL-4 were significantly decreased(P<0.01).In high and medium dose group, the percentages of CD4+T was (34.81±3.80)%,(34.92±5.14)%,the ratio of CD4+T/ CD8+T was 2.21±0.43,2.27±0.48,respectively,compared with model group they were significantly decreased(P<0.05,P<0.01).In dihydroartemisinin three dose group, the levels of INF-γ were (15.90±2.05),(16.27±2.11),(18.15±2.15) ng ·L-1, and the levels of IL-4 were (40.21±4.89),(40.04±4.56),(34.81±4.02) ng ·L-1,compared with the model group they had significant changes(P<0.01).For IL-4,dihydroartemisinin’s effect showed a dose-dependent manner at concentrations between 2.8-5.6 mg · kg-1(P<0.05). Conclusion: Dihydroartemisinin could effectively improve the immunity mess in T-lymphocytes from peripheral blood in AA rats, which is useful for curing rheumatoid arthritis.关键词：dihydroartemisinine;T-lymphocytes;AA
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发布时间：2024-09-23 - 摘要：Objective:To study the effect of effective parts from Musa basjoo（EPM） on blood glucose and glucose tolerance in mice.Method: Diabetic model was induced by intraperitoneal injection of Alloxan once.Diabetic mice were randomly divided into model group,Metformin group and high,middle and low dose of EPM groups（200,100,50 mg · kg-1）.All groups were fed for 14 consecutive days,and then the blood glucose was detected on the fifteenth day.Effect on the glucose tolerance in model and normal mice were detected at the same time.Result: EPM could significantly decrease the blood glucose level of diabetic mice（P<0.05）and ameliorate the glucose tolerance in normal and diabetic mice（P<0.05）,but it had no significant effect on the blood glucose level of normal mice.Conclusion: EPM can significantly decrease the blood glucose level in diabetic mice and ameliorate the glucose tolerance in normal and diabetic mice.关键词：Musa basjoo;effective parts;blood glucose;glucose tolerance
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发布时间：2024-09-23 - 摘要：Objective: To study in vitro bacteriostasis of ethanol extract from the rhizomes of Zingiber striolatum Diels. Method: The rhizomes were percolated with ethanol and then the residue was sequentially extracted with petroleum ether, chloroform, ethyl acetate and n-butanol after reduce-pressure distillation. The ethanol extract and various polar fractions were screened for in vitro antibacterial activities by disc-diffusion method. The minimum inhibition concentrations (MICs) and minimum bacteriocidal concentrations (MBCs) were further determined for active polar fractions by broth microdilution method. Result: The crude ethanol extract (CEE), petroleum ether-and chloroform soluble-parts (PE and CE, resp.) inhibited growth of Gram-positive (Staphylococcus aureus, Baicllus licheniformis and Streptococcus agalactiae) and Gram-negative (Escherichia coli,Klebsiella pneumonia and Pseudomonas aeruginosa) with MICs ranging from 312.5 mg · L-1 to 1 250 mg · L-1. The MBCs of CEE against B. licheniformis, S. agalactiae and E. coli were 1 250 mg · L-1, while those of PE and CE against all the test strains varied from 625 mg · L-1 to 1 250 mg · L-1. Conclusion: The CEE, PE and CE from the rhizomes of Z. striolatum possess antibacterial and bacteriocidal activities against S. aureus, B. licheniformis, S. agalactiae, E. coli,K. pneumonia and P. aeruginosa.关键词：Zingiberaceae;Zingiber striolatum;rhizoma;bacteriostasis
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发布时间：2024-09-23 -
Effects of Astragalus Injection on Pathological Changes of Renal Damage in Type 2 Diabetic KKAy Mice
摘要：Objective:To observe protective effects of Astragalus injection on renal function,morphological changes in type 2 diabetic KKAy mice.Method:Male KKAy mice were randomly divided into model group,Astragalus injection treatment group（ip,3 mL·kg-1·d-1）,the same age male C57BL/6J mice were selected as normal control group.Blood glucose regularly,blood urea and serum creatinine at 20,24 and 28 weeks of age were measured respectively.Light microscopy and electron microscope were used to observ renal pathological changes in mice of different ages.Result: At 20 weeks of age,blood glucose and blood urea levels in model group KKAy mice were significantly higher than the normal mice（P<0.01）.At 24 and 28 weeks of age,serum creatinine levels were significantly higher than the normal mice（P<0.01）.At Astragalus injection treatment group KKAy mice with 20 weeks of age,blood glucose was significantly higher than that of normal group（P<0.01）,but lower than the model group mice（P<0.05 or P<0.01）.At 24 and 28 weeks of age,Astragalus injection treatment group KKAy mice serum creatinine level was significantly lower than model group mice（P <0.01）;and from 20 weeks of age,blood urea levels were significantly lower than the model group mice（P <0.01）,but compared with normal group,no significant difference was found.Model group KKAy mice showed widened mesangial areas,basement membrane thickening,vacuoles in renal tubular epithelial cells,increased renal interstitial connective tissue and other pathological changes from 20 weeks of age,lesions with ages increased.Lesions in the mice treated by Astragalus injection were markedly improved.Conclusion: Astragalus injection can improve kidney function,reduced pathological changes in kidney of KKAy mice with type 2 diabetes.关键词：KKAy mice;Astragalus injection;diabetic nephropathy;pathological changes- 0
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发布时间：2024-09-23 - 摘要：Objective: To study the mechanism of Shehuang cream on prevention and curing of eczema. Method: Seventy male mice were randomized to control group,model group, Asmanex group, different concentration groups of Shehuang cream,mice delayed hypersensitivity reaction was induced by dinitrofluobenzene;mice pinna and paw swelling were induced by carrageenan to investigate inhibition rate on tumefaction, and the glacial acetic acid induced capillary permeability model was used to evaluate the effect of Shehuang cream and explore it’s mechanism. Sixty SD rats were randomized to control group,model group,Ioratadine group, different concentration groups of Shehuang cream,0.1% phosphoric acid histamine was used to detect the degree of clarification. Sixty-five guinea-pigs were divided into differents groups,guinea pig pruritus were induced by histamine phosphate. Result: Shehuang cream could significantly inhibit the delayed hypersensitivity induced by 2,4-dinitrofluorobanzene(DNFB), but has no effect on the indexes of thymus and spleen in mice. Shehuang cream could relieve the itch responses in guinea pig and skin permeability in rat induced by histamine(P<0.01);it also inhibited the auricula inflammation induced by of xylene and enterocoelia capillary permeability by glacial acetic acid in mice(P<0.01), but had a little action on the paw swelling induced by carrageenan in mice. Conclusion: The results indicte that Shehuang cream’s antipruritic effect may be related to regulation of immune function, antagonistic action on histamine and reducing the inflammatory reaction.关键词：Shehuang cream;allergy;anti-inflammatory;eczema
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发布时间：2024-09-23 - 摘要：Objective:To investigate the protective effects and its mechanism of Supplemental Sini San（SNS） on hepatic fibrosis in rats.Method:The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum.Eighty Wister rats were divided into eight groups:normal control,model control,Sini San Jiawei（SNSJW） high-dose,mediate-dose and low-dose,prevention and SNS group.Except the normal control groups,all the other groups were injected with pig’s serum without inactivation into abdominal cavity,twice per week and 0.5 mL each time,persisting for 10 weeks.The prevention groups were orally given with the medicines（SNSJW 7 g · kg-1,10 weeks）at the same time models wasduplicated,the other treatment groups were dealed with just as the prevention groups 6 weeks later,lasted for 4 weeks.The dose was following:SNS group 4 g · kg-1,SNSJW high-dose group 14 g · kg-1,mediate-dose group 7 g · kg-1,low-dose group 3.5 g · kg-1.The changes of hepatic fibrosis were detected.The content of liver tissue interleukin-1（IL-1） and tumor necrosis factor-alpha（TNF-α）were tested by RIA.In situ hybridization assay and immunohistochemical S-P method were used to detect the expression of transforming growth factor-β1（TGF-β1） mRNA and α-smoth muscle actin（α-SMA） mRNA and there protein in hepatic fibrosis tissues.Result:Compared with model group in SNSJW mediate-dose group,the content of HYP,IL-1,TNF-α in liver tissue was depressed significantly,the expression of TGF-β1 and α-SMA genes was significantly lower in SNSJW mediate-dose group.Conclusion:Supplemental SNS has an action on hepatic fibrosis in rats.The mechanism is related with its inhibiting the expression of TGF-β1 and α-SMA genes in rats liver.关键词：Sini San;liver fibrosis;transforming growth factor-β1;α-smooth muscle actin
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发布时间：2024-09-23 - 摘要：Objective: In order to investigate the effects of Xianti JiangzhiⅠPrescription in proventing obeseing induced by ovariectory in ratsand explore the possible mechanism. Method: Seventy female SD rats, weighting about 270 g were randomly divided into 7 groups:sham-operated group (sham),simple ovariectomized group and three treated groups with low(3.3 g · kg-1 · d-1),middle(6.6 g · kg-1 · d-1) and high(13.2 g · kg-1 · d-1) dosage of Xianti JiangzhiⅠprescription, diethyllstillbestrol group(2.2×10-5g · kg-1 · d-1),trimetazidine group(8.9×10-4g · kg-1 · d-1),ten each group. The administration started 10 days after operation and continued for 3 weeks. In the end, the blood samples were collected to measure following indexes:serum estradiol (E2), blood lipids, leptin,wet weight of fat, et al. Result: Xianti JiangzhiⅠprescription can significantly inhibit the increase of weight and food intake in the model rats(P<0.05 or P<0.01),decreased the weight of wet abdominal fat, reduce the lees indexes(P<0.05 or P<0.01), significantly prevented obesity induced by ovariectory, significantly decreased the TG,TC,LDL-C level(P<0.05 or P<0.01)and increased the level of blood estrogen(P<0.05 or P<0.01). Conclusion: Xianti JiangzhiⅠprescription can significantly prevent obesity induced by ovariectory by;decreasing the blood lipids and increasing the level of blood Estrogen, this may be one of its mechanisms.关键词：obesity;Xianti JiangzhiⅠprescription;ovariectomized rats;effect mechanism.
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发布时间：2024-09-23 - 摘要：Objective:The aim of our study is to investigate the effect of Yulangsan flavonoids（YLSF） against CCl4-induced liver fibrosis in rats and study its mechanism.Method:The SD rats were divided into two groups randomly:hepatic fibrosis group and normal control group（NC）.The rats of hepatic fibrosis group were induced by intragastric administration（ig） of 50% CCl4,and rats in NC group were given normal saline（NS）.The rats of hepatic fibrosis group confirmed by the pathological inspection were divided into 2 groups randomly:Drug intervention group and the model group which were treated with NS.The drug intervention group was divided randomly into 3 different does YLSF groups（20,40,80 mg · kg-1） and a positive control group（colchicine tablets 0.20 mg · kg-1）.All rats were treated with drugs or NS for four consecutive weeks ig,one time a day.Twenty-four hours after the last administration of drugs,all rats were sacrificed,and the blood serum and hepatic tissue were taken quickly.The activities of alanine transaminase（ALT） and aspartale transaminase（AST） in the serum and the levels of superoxide dismutase（SOD）,malonaldehyde（MDA）,glutathione（GSH）,and glutathione synthesis（GSH-Px） in hepatic tissue were analyzed,and the degree of hepatic injury was examined.Result:The content of AST,ALT in serum of each YLSF groups’ rats were（207.85±101.72）,（131.55±35.09）,（129.98±37.21） U · L-1,（90.51±44.24）,（47.79±16.11）,（44.56±16.31） U · L-1;and the content of SOD,MDA,GSH-Px,GSH in the tissue of each YLSF groups’ rats were（143.14±42.82）,（146.53±31.98）,（147.41±32.82） U · mg-1,（2.57±0.54）,（2.19±0.57）,（2.11±0.59） nmol · mg-1,（463.55±271.07）,（659.14±162.23）,（752.08±200.70） nmol · mg-1,（5.06±1.09）,（6.10±0.97）,（6.89±0.98） nmol · mg-1.Compared with the model control group,the increased activity of AST in serum and the increased content of MDA in liver induced by CCl4 were decreased significantly,and the decreased levels of SOD in liver was increased significantly in all dose of YLSF groups and positive control group.The activity of ALT in serum was decreased significantly（P<0.01） and the content of GSH（P<0.01） in liver was increased in the high dose and the middle dose of YLSF groups and positive control group.Meanwhile the activity of GSH-Px in the liver was increased significantly in the high dose and the middle dose of YLSF groups（P<0.01）.The degree of hepatic injury in all dose of YLSF groups and positive control group could be lessened（P<0.05 or P<0.01）.Conclusion:Our findings demonstrate that YLSF has a certain curative effect on CCl4-induced liver fibrosis in rats,and its mechanism maybe involve in attenuating free radical and inhibiting the lipid peroxidation.关键词：Yulangsan;flavonoids;liver fibrosis
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发布时间：2024-09-23 -
Effect of Dihydroartemisinin on Immune-regulation of T-lymphocytes from Peripheral Blood in AA Rats.
摘要：Objective:To study the effect of dihydroartemisinin on immune-regulation of T-lymphocytes from peripheral blood in adjuvant-induced arthritis（AA） rats.Method: Sprague Dawley rats with a mean weight of 160-180 g were randomly divided into 6 groups,including normal group,AA model group,methotrexate group（1 mg · kg-1）,dihydroartemisinine high-dose,medium-dose and low-dose group（11.2,5.6,2.8 mg ·kg-1）.The rat adjuvant arthritis model were induced.The drugs were administrated by ig for consecutive 28 days after inducing AA model.T-lymphocyte in peripheral blood was detected by flow cytometry,and the levels of interferon-γ（INF-γ） and interleukin-4（IL-4） in serum were measured by ELISA on AA animal model treated bydihydroartemisinin.Result: Compared with the normal group,the ratio of CD4+T,CD4+T/ CD8+T from peripheral blood in model group and IFN-γ levels in serum were significantly increased（P<0.01）,and the levels of IL-4 were significantly decreased（P<0.01）.In high and medium dose group,the percentages of CD4+T was（34.81±3.80）%,（34.92±5.14）%,the ratio of CD4+T/ CD8+T was 2.21±0.43,2.27±0.48,respectively,compared with model group they were significantly decreased（P<0.05,P<0.01）.In dihydroartemisinin three dose group,the levels of INF-γ were（15.90±2.05）,（16.27±2.11）,（18.15±2.15） ng·L-1,and the levels of IL-4 were（40.21±4.89）,（40.04±4.56）,（34.81±4.02） ng·L-1,compared with the model group they had significant changes（P<0.01）.For IL-4,dihydroartemisinin’s effect showed a dose-dependent manner at concentrations between 2.8-5.6 mg · kg-1（P<0.05）.Conclusion:Dihydroartemisinin could effectively improve the immunity mess in T-lymphocytes from peripheral blood in AA rats,which is useful for curing rheumatoid arthritis.关键词：dihydroartemisinine;T-lymphocytes;AA- 0
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发布时间：2024-09-23 - 摘要：Objective: To investigate the influence of quercetin on the apoptosis of hepatic cancer cells SMMC-7721 and on PI3K/AKT signal pathways in SMMC-7721 cells. Method: Inhibitory effect of quercetin on SMMC-7721 cell growth was detected by MTT assay. Cell cycle changes were analyzed by flow cytometry (FCM). The Western blot test was employed for evaluating influence of quercetin on apoptosis related protein expression in PI3K/AKT signal pathways of SMMC-7721. Result: Quercetin inhibited cell proliferation of SMMC-7721 obviously, and in a concentration and time dependence manner. The inhibition rates of cisplatin and quercetin (40,80,160,320 μmol · L-1, 48 h) were 62.19%, 25.47%, 27.18%, 36.96%, 51.28% respectively. FCM showed that 80,160,320 μmol · L-1 of quercetin could arrest SMMC-7721 cells in G0/G1 phase. Western blot showed that apoptosis related protein expression testing showed that the expression of AKT inhibited in the drug groups, and the protein expression of PTEN, caspase-9 increased with the drug concentration. Conclusion: Quercetin can induce apoptosis of SMMC-7721 cell, its mechanism could be related to hepatic cancer cells SMMC-7721 arrested in G0/G1 phase, the inhibition of AKT activation by PTEN gene over-expression, and the promotion of apoptosis by caspase-9 activation.关键词：quercetin;hepatic carcinoma cell lines SMMC-7721;PI3K/AKT signal pathway;apoptosis
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发布时间：2024-09-23 - 摘要：Objective:To study in vitro bacteriostasis of ethanol extract from the rhizomes of Zingiber striolatum Diels.Method:The rhizomes were percolated with ethanol and then the residue was sequentially extracted with petroleum ether,chloroform,ethyl acetate and n-butanol after reduce-pressure distillation.The ethanol extract and various polar fractions were screened for in vitro antibacterial activities by disc-diffusion method.The minimum inhibition concentrations（MICs） and minimum bacteriocidal concentrations（MBCs） were further determined for active polar fractions by broth microdilution method.Result:The crude ethanol extract（CEE）,petroleum ether-and chloroform soluble-parts（PE and CE,resp.） inhibited growth of Gram-positive（Staphylococcus aureus,Baicllus licheniformis and Streptococcus agalactiae） and Gram-negative（Escherichia coli,Klebsiella pneumonia and Pseudomonas aeruginosa） with MICs ranging from 312.5 mg · L-1 to 1 250 mg · L-1.The MBCs of CEE against B.licheniformis,S.agalactiae and E.coli were 1 250 mg · L-1,while those of PE and CE against all the test strains varied from 625 mg · L-1 to 1 250 mg · L-1.Conclusion:The CEE,PE and CE from the rhizomes of Z.striolatum possess antibacterial and bacteriocidal activities against S.aureus,B.licheniformis,S.agalactiae,E.coli,K.pneumonia and P.aeruginosa.关键词：Zingiberaceae;Zingiber striolatum;rhizoma;bacteriostasis
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- 269
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发布时间：2024-09-23 - 摘要：Objective: To investigate effects of Erzi Capsule (EZC) on apoptosis genes in rats with spermatogenic failure and to explore the related mechanism of action for treatment of male infertility. Method: Forty male rats were randomly divided in to 4 groups:model group, control group and two EZC dose groups (4.8,2.4 g · kg-1). The rat model of spermatogenic failure was established by intraperitoneal injection of cyclophosphamide (40 mg · kg-1). The treatments were lasted for 60 days. TUNEL method was used to determine apoptosis in spermatogenic cells. Bax and Bcl-2 expressions in testis were tested by immunohistochemical method. Result: Compared with the model group, two doses of EZC group decreased apoptosis index, lowered Bax expression and increased Bcl-2 expression significantly(P<0.01). Conclusion: EZC could downregulate the expression of Bax and upregulation Bcl-2 expression, reduce the incidence of apoptosis in spermatogenic cells.关键词：Erzi Capsule;spermatogenic failure;cell apoptosis
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发布时间：2024-09-23 - 摘要：Objective:To study the mechanism of Shehuang cream on prevention and curing of eczema.Method:Seventy male mice were randomized to control group,model group,Asmanex group,different concentration groups of Shehuang cream,mice delayed hypersensitivity reaction was induced by dinitrofluobenzene;mice pinna and paw swelling were induced by carrageenan to investigate inhibition rate on tumefaction,and the glacial acetic acid induced capillary permeability model was used to evaluate the effect of Shehuang cream and explore it’s mechanism.Sixty SD rats were randomized to control group,model group,Ioratadine group,different concentration groups of Shehuang cream,0.1% phosphoric acid histamine was used to detect the degree of clarification.Sixty-five guinea-pigs were divided into differents groups,guinea pig pruritus were induced by histamine phosphate.Result:Shehuang cream could significantly inhibit the delayed hypersensitivity induced by 2,4-dinitrofluorobanzene（DNFB）,but has no effect on the indexes of thymus and spleen in mice.Shehuang cream could relieve the itch responses in guinea pig and skin permeability in rat induced by histamine（P<0.01）;it also inhibited the auricula inflammation induced by of xylene and enterocoelia capillary permeability by glacial acetic acid in mice（P<0.01）,but had a little action on the paw swelling induced by carrageenan in mice.Conclusion:The results indicte that Shehuang cream’s antipruritic effect may be related to regulation of immune function,antagonistic action on histamine and reducing the inflammatory reaction.关键词：Shehuang cream;allergy;anti-inflammatory;eczema
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发布时间：2024-09-23 - 摘要：Objective: To investigate protective effects of the compatibility of paeonol and paeoniflorin on experimental myocardial ischemia in rats. Method: Using the method of ligation of left descending coronary artery the rat model of myocardial ischemia was established. Experiment group:sham group, model group, control group, paeonol 100 mg · kg-1+paeoniflorin 25 mg · kg-1, paeonol 50 mg · kg-1 +paeoniflorin 12.5 mg · kg-1, paeonol 25 mg · kg-1 +paeoniflorin 6.25 mg · kg-1. ig 10 mL · kg-1 for 5 d. Sham group and model group was given ig physiological saline. electrocardiogram(ECG), aspartate, transaminase(AST), crealine kinase(CK), lactate dehydrogenase(LDH) and the myocardial infarct size were measured. Result: Compared with the model group, the S-T segment and T waves elevation levels in myocardial ischemia rats in the medium dose and high dose groups of the compatibility of paeonol and paeoniflorin were obviously reduced(P<0.05 or P<0.01).The myocardial infarct sizes were decreased(P<0.05 or P<0.01). The content of AST, CK and LDH in rat serum were reduced. Conclusion: The compatibility of paeonol and paeoniflorin can effectively protect myocardial ischemic injury of rats.关键词：paeonol;paeoniflorin;compatibility;myocardial ischemia
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发布时间：2024-09-23 - 摘要：Objective:In order to investigate the effects of Xianti JiangzhiⅠPrescription in proventing obeseing induced by ovariectory in ratsand explore the possible mechanism.Method:Seventy female SD rats,weighting about 270 g were randomly divided into 7 groups:sham-operated group（sham）,simple ovariectomized group and three treated groups with low（3.3 g · kg-1 · d-1）,middle（6.6 g · kg-1 · d-1） and high（13.2 g · kg-1 · d-1） dosage of Xianti JiangzhiⅠprescription,diethyllstillbestrol group（2.2×10-5g · kg-1 · d-1）,trimetazidine group（8.9×10-4g · kg-1 · d-1）,ten each group.The administration started 10 days after operation and continued for 3 weeks.In the end,the blood samples were collected to measure following indexes:serum estradiol（E2）,blood lipids[total cholesterol（TC）,trigly cerides（TG）,high density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）],leptin,wet weight of fat,et al.Result:Xianti JiangzhiⅠprescription can significantly inhibit the increase of weight and food intake in the model rats（P<0.05 or P<0.01）,decreased the weight of wet abdominal fat,reduce the lees indexes（P<0.05 or P<0.01）,significantly prevented obesity induced by ovariectory,significantly decreased the TG,TC,LDL-C level（P<0.05 or P<0.01）and increased the level of blood estrogen（P<0.05 or P<0.01）.Conclusion:Xianti JiangzhiⅠprescription can significantly prevent obesity induced by ovariectory by;decreasing the blood lipids and increasing the level of blood Estrogen,this may be one of its mechanisms.关键词：obesity;Xianti JiangzhiⅠprescription;ovariectomized rats;effect mechanism
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- 73
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发布时间：2024-09-23 - 摘要：Objective: To investigate 1,1-diphenyl-2-picrylhydrazy1(DPPH) free radical scavenging activities of extracts from Orobanche cernua var. cumana. Method: The 70% ethanol extracts from O. cernua var. cumana was extracted successively with ethyl acetate and n-butanol. The three fractions of ethyl acetate,n-butanol,and water were tested using assays of the scavenging effects on DPPH. The model of oxidative damage of human embryonic kidney cell line HEK-293 cells was induced by H2O2,MTT assay was used to evaluate the cell viability. Result: The IC50 in DPPH test were in the following orders:ethyl acetate(0.042 9 g · L-1)关键词：Orobanche cernua var. cumana;DPPH free radical scavenging activities;human embryonic kidney 293 cells
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发布时间：2024-09-23 - 摘要：目的:观察化痰通络法对急性脑梗死大鼠重组组织纤溶酶原激活剂（rt-PA）溶栓后神经功能缺损评分及神经元微观形态的影响,为化痰通络法防治急性脑梗死溶栓后缺血再灌注损伤提供实验依据。方法:选择健康雄性SD大鼠80只,随机分为3组,即假手术组、模型组、化痰通络法联合rt-PA组（每组又分6,24 h,3,7 d 4个时相）,采用自身栓子法制备大鼠大脑中动脉栓塞模型（MCAO）,化痰通络法结合rt-PA组于血栓注入后3 h一次性由尾静脉缓慢注入2 mL 0.9%生理盐水+5.67 mg.kg-1rt-PA,并分别给予高、低剂量化痰通络方灌胃治疗（2.88,1.44 g.kg-1）,每日2次。对大鼠不同时相进行神经功能缺损评分,并通过光电镜对大脑皮质缺血区神经元进行形态观察。结果:Bederson’s评分:与模型组相比,治疗组神经功能缺损评分明显降低（P<0.01）,其中以rt-PA联合化痰通络方低、高剂量组神经功能缺损评分降低最为明显（P<0.05）。光镜下,在同一时相中,与模型组相比,治疗组皮质神经元少量变性,组间并无显著差别。同组不同时相比较,以7 d神经元变性最多。电镜下,在同一时相中,与模型组相比,治疗组均起到保护神经元及内皮细胞的作用,但以化痰通络方联合rt-PA组更为显著。同组不同时相比较,以24 h神经元水肿最为严重,7 d神经元破坏最为严重。各组横向比较,以化痰通络方联合rt-PA高剂量组24 h时相对神经元的保护作用最好,化痰通络方联合rt-PA低剂量组3 d和7 d对神经元形态保护为最佳。结论:化痰通络法可以减轻急性脑梗死溶栓后神经功能缺损症状,改善皮质微观形态学变化,同时针对溶栓后不同时相调整中药的用药剂量,可以更好的减轻溶栓后缺血再灌注损伤。关键词：化痰通络法;脑梗死;溶栓;神经元;形态学
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- 186
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发布时间：2024-09-23 - 摘要：Objective: To investigate the antioxidant effects of addition and subtraction Bufei decoction (ASBD) on pulmonary fibrosis (PF) in rats. Method: Forty male Sprague-Dawley rats were divided randomly into sham operation group, model group,ASBD group. The PF model was reproduced by intratracheal injection of bleomycin. ASBD group was given traditional Chinese medicine decoction (ig, 2 times to clinic dose, 16 g · kg-1) starting from 2 days after operation. The sham operation group and model group were given the same amount of normal saline. The blood and lung tissue of rats were took on the 14 d and 28 d. The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum were measured. The content of hydroxyproline in lung tissue was detected. The mRNA of extracellular SOD(ECSOD) was measured by real-time PCR. Result: ASBD significantly improved the survival state of PF rats, reduced content of hydroxyproline in the lung tissue, decreased the level of MDA in the serum, improved the expression of ECSOD mRNA. Conclusions: ASBD could improve the survival rate of PF rats, and the fibrosis index. The mechanism might be related to decrease in the level of serum MDA and up-regulation of the expression of ECSOD mRNA.关键词：Addition and Subtraction Bufei decoction;superoxide dismutase;idiopathic fibrosis;antioxidant
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发布时间：2024-09-23 - 摘要：Objective:To investigate the influence of quercetin on the apoptosis of hepatic cancer cells SMMC-7721 and on PI3K/AKT signal pathways in SMMC-7721 cells.Method:Inhibitory effect of quercetin on SMMC-7721 cell growth was detected by MTT assay.Cell cycle changes were analyzed by flow cytometry（FCM）.The Western blot test was employed for evaluating influence of quercetin on apoptosis related protein expression in PI3K/AKT signal pathways of SMMC-7721.Result:Quercetin inhibited cell proliferation of SMMC-7721 obviously,and in a concentration and time dependence manner.The inhibition rates of cisplatin and quercetin（40,80,160,320 μmol · L-1,48 h） were 62.19%,25.47%,27.18%,36.96%,51.28% respectively.FCM showed that 80,160,320 μmol · L-1 of quercetin could arrest SMMC-7721 cells in G0/G1 phase.Western blot showed that apoptosis related protein expression testing showed that the expression of AKT inhibited in the drug groups,and the protein expression of PTEN,caspase-9 increased with the drug concentration.Conclusion:Quercetin can induce apoptosis of SMMC-7721 cell,its mechanism could be related to hepatic cancer cells SMMC-7721 arrested in G0/G1 phase,the inhibition of AKT activation by PTEN gene over-expression,and the promotion of apoptosis by caspase-9 activation.关键词：quercetin;hepatic carcinoma cell lines SMMC-7721;PI3K/AKT signal pathway;apoptosis
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- 375
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发布时间：2024-09-23 - 摘要：Objective: To compare the analgesic effect and effective fractions of wild and cultivated Psammosilene tunicoides. Method: compounds of different polarity from roots of wild and cultivated P. tunicoides were extracted, hot plate test and acetic acid writhing test were used in mice for evaluating analgesic effect of the extracts. Result: The extracts of wild and cultivated P. tunicoides produced the similar analgesic effect;both of them contained highly active analgesic ingredients. For the inhibitory effect of the extracts on chemical stimulation induced pain, the active ingredients of wild and cultivated P. tunicoides were found in the parts of n-butanol, ethyl acetate and petroleum. But the ethyl acetate part of artificial cultivation sample had a significant analgesic activity better than that of the wild sample (P<0.05). For the analgesic effect on thermal stimulation induced pain model, the effective fractions of wild P. tunicoides was the n-butanol part, the n-butanol and ethyl acetate parts were in cultivation sample. Conclusion: The artificial cultivation and wild herbs of P. tunicoides, haas the different active ingredients of analgesic function, but their analgesic efficacy do not show a significant difference. So the cultivated P. tunicoides could be used as the equivalent analgesic medicine to substitute the wild herbs.关键词：Psammosilene tunicoides;wild;cultivation;analgesia;effective fractions
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发布时间：2024-09-23 - 摘要：Objective:To investigate effects of Erzi Capsule（EZC） on apoptosis genes in rats with spermatogenic failure and to explore the related mechanism of action for treatment of male infertility.Method:Forty male rats were randomly divided in to 4 groups:model group,control group and two EZC dose groups（4.8,2.4 g · kg-1）.The rat model of spermatogenic failure was established by intraperitoneal injection of cyclophosphamide（40 mg · kg-1）.The treatments were lasted for 60 days.TUNEL method was used to determine apoptosis in spermatogenic cells.Bax and Bcl-2 expressions in testis were tested by immunohistochemical method.Result:Compared with the model group,two doses of EZC group decreased apoptosis index [（7.16±1.47）%,（13.83±2.40）% vs（20.50±2.17）%],lowered Bax expression [（16.01±2.02）,（26.67±1.79） vs（9.94±1.14）] and increased Bcl-2 expression [（40.12±3.06）,（22.87±2.94） vs（16.26±1.03）] significantly（P<0.01）.Conclusion:EZC could downregulate the expression of Bax and upregulation Bcl-2 expression,reduce the incidence of apoptosis in spermatogenic cells.关键词：Erzi Capsule;spermatogenic failure;cell apoptosis
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发布时间：2024-09-23 - 摘要：Objective: To determine the content of tannins in above and under ground parts of scorzonera sinensis. To observe antioxidative activity of tannin from scorzonera sinensis. Method: The complexometric titration was used to determine the content of tannins. Iodometric and spectrophotometry were used to determine the effects of tannins on H2O2 and · OH. Result: The tannins in above and under ground parts of scorzonera sinensis was 8.18% and 9.27% accordingly. With the Concentration of 0.05 g · mL-1, the clearance rates on H2O2 and · OH were 8.89% and 88.24%. Conclusion: Tannins of scorzonera sinensis have a strong ability to resistance oxidation.
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发布时间：2024-09-23 - 摘要：Objective:To investigate protective effects of the compatibility of paeonol and paeoniflorin on experimental myocardial ischemia in rats.Method:Using the method of ligation of left descending coronary artery the rat model of myocardial ischemia was established.Experiment group:sham group,model group,control group,paeonol 100 mg · kg-1+paeoniflorin 25 mg · kg-1,paeonol 50 mg · kg-1 +paeoniflorin 12.5 mg · kg-1,paeonol 25 mg · kg-1 +paeoniflorin 6.25 mg · kg-1.ig 10 mL · kg-1 for 5 d.Sham group and model group was given ig physiological saline.electrocardiogram（ECG）,aspartate,transaminase（AST）,crealine kinase（CK）,lactate dehydrogenase（LDH） and the myocardial infarct size were measured.Result:Compared with the model group,the S-T segment and T waves elevation levels in myocardial ischemia rats in the medium dose and high dose groups of the compatibility of paeonol and paeoniflorin were obviously reduced（P<0.05 or P<0.01）.The myocardial infarct sizes were decreased（P<0.05 or P<0.01）.The content of AST,CK and LDH in rat serum were reduced.Conclusion:The compatibility of paeonol and paeoniflorin can effectively protect myocardial ischemic injury of rats.关键词：paeonol;paeoniflorin;compatibility;myocardial ischemia
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- 180
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发布时间：2024-09-23 - 摘要：Objective: To evaluate the acute toxicity and the protective effects of Radix Adenophorae Potanini Korsh Polysaccharides dispersible tablets (RPDTS) on acute hepatic injury induced by carbon tetrachloride ( CCl4) in mice. Method: The maximum dosage test was used as intragastric administration in mice to observe acute toxicity of RPDTS. After intragastric administration RPDTS(0.75,1.5,3 g · kg-1)in mice for 7 days, the acute liver injury model was induced by CCl4. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) in serum and total superoxide dismutase(T-SOD), malondialdehyde(MDA), glutathione peroxideobjects enzyme(GSH-Px) in liver were determined by kits respectively. Histopathological examination was also carried out. Result: Acute toxicity test showed that the total dose of intragastric administration of RPDTS was 208 g · kg-1 (according to Adenophorae tetraphylla) equivalent to 120 times the clinical dose for adults. Acute liver injury test showed that RPDTS could obviously decrease the levels of ALT, AST in serum and MDA in liver(P<0.05), obviously increase the levels of T-SOD, GSH-Px in liver,the histopathological examination indicated that liver injuries were relieved. Conclusion: RPDTS has no acute toxicity,RPDTS has protective effects on acute liver injuries induced by CCl4 in mice.关键词：Radix Adenophorae Potanini Korsh Polysaccharides;dispersible tablets;acute toxicity;acute liver injury;CCl4;the maximum dosage test
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发布时间：2024-09-23 - 摘要：Objective:To investigate 1,1-diphenyl-2-picrylhydrazy1（DPPH） free radical scavenging activities of extracts from Orobanche cernua var.cumana.Method:The 70% ethanol extracts from O.cernua var.cumana was extracted successively with ethyl acetate and n-butanol.The three fractions of ethyl acetate,n-butanol,and water were tested using assays of the scavenging effects on DPPH.The model of oxidative damage of human embryonic kidney cell line HEK-293 cells was induced by H2O2,MTT assay was used to evaluate the cell viability.Result:The IC50 in DPPH test were in the following orders:ethyl acetate（0.042 9 g · L-1）<n-butanol（0.059 9 g · L-1）<VC（0.071 8 g · L-1）<water（0.330 3 g · L-1）.The ethyl acetate fraction showed the strongest scavenging activity to DPPH free radicals.The three fractions of ethyl acetate,n-butanol and water could significantly improve the survival rate of injured HEK-293 cells,showing the antioxidant activities.Conclusion:These results demonstrate that the extracts from O.cernua var.cumana have some anti-oxidant and free radical scavenging activities.关键词：Orobanche cernua var.cumana;DPPH free radical scavenging activities;human embryonic kidney 293 cells
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发布时间：2024-09-23 - 摘要：Objective: To investigate the poctect function of Diyu Shengbai tabletts on bone marrow depression induced by cyclophosphamide in mice. Method: Twenty-four Balb/C mice, half male and femal, were categorized into three groups. Mice in model group and Diyu Shengbai group were administered cyclophosphamide (Cy) (100 mg · kg-1 once a day for 3 days by intraperitoneal injection) to induce marrow suppression. Diyu Shengbai group was received Diyu Shengbai table treatment (100 mg · kg-1body weight orally once a day for 10 days). A vehicle treated control group was also arrangeed. The changes of peripheral hemogram, bone marrow nucleated cells (BMCs), DNA content and bone marrow cell cycle were detected. Result: Cy-treated mice showed a significant decrease in the number of white blood cells, thrombocyte, bone marrow nucleated cell proliferation and the content of DNA in bone (compared with control group, there were significant differences,P<0.05 or P<0.01).The percentage of G0/G1 phase was increased and the percentage G2/M phase decreased (compared with control group, there were significant differences,P<0.05 or P<0.01). Diyu Shengbai tablets significantly increased the number of white blood cells, thrombocyte, bone marrow nucleated cell proliferation and markedly increased the percentage of G2/M phase(compared with model group (P<0.05 or P<0.01). Conclusion: Diyu Shengbai Tablets could improve the hemopoietic function for marrow suppression induced by Cy in mice.关键词：Diyu Shengbai tablets;cyclophosphamide;bone marrow depression;cell cycle;bone marrow nucleated cells
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发布时间：2024-09-23 - 摘要：Objective:To investigate the antioxidant effects of addition and subtraction Bufei decoction（ASBD） on pulmonary fibrosis（PF） in rats.Method:Forty male Sprague-Dawley rats were divided randomly into sham operation group,model group,ASBD group.The PF model was reproduced by intratracheal injection of bleomycin.ASBD group was given traditional Chinese medicine decoction（ig,2 times to clinic dose,16 g · kg-1） starting from 2 days after operation.The sham operation group and model group were given the same amount of normal saline.The blood and lung tissue of rats were took on the 14 d and 28 d.The superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in serum were measured.The content of hydroxyproline in lung tissue was detected.The mRNA of extracellular SOD（ECSOD） was measured by real-time PCR.Result:ASBD significantly improved the survival state of PF rats,reduced content of hydroxyproline in the lung tissue,decreased the level of MDA in the serum,improved the expression of ECSOD mRNA.Conclusions:ASBD could improve the survival rate of PF rats,and the fibrosis index.The mechanism might be related to decrease in the level of serum MDA and up-regulation of the expression of ECSOD mRNA.关键词：Addition and Subtraction Bufei decoction;superoxide dismutase;idiopathic fibrosis;antioxidant
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- 202
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发布时间：2024-09-23 - 摘要：Objective: To study the effects of 17-hydroxy-jolkinolide B (HJB) on proliferation and apoptosis of U251 cells. Method: After the cells were treated with HJB at various concentrations (0, 6.25, 12.5,25,50,100,200,400,800 μmol ·L-1)for 24 h, or at half maximal inhibitory concentration of a substance (IC50) for various durations(12, 24, 48, 72 h), cell viability was measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT). Annexin V-FITC by cytometry was used to test the apoptosis rate. Absorption spectrometry was adopt to measure the relative activity of Caspase-3 and Caspase-9. Result: The HJB evidently inhibited the growth of U251 cells in a time and dose-dependent manner(P<0.05), with IC50value for 24 h being 62.236 11 μmol · L-1, and it could induce apoptosis of U251 cells. After HJB treatment at 30, 60 and 120 μmol · L-1for 24 h, the apoptosis rate of U251 cells was 3.10%, 3.51% and 5.98%, respectively(P<0.05), and the relative activity of Caspase-3 or Caspase-9 was advanced with dose-dependent manner. Conclusion: The HJB evidently inhibits the growth of U251 and induces the apoptosis of U251.The mechanism may be mediated by mitochondrion pathways.关键词：17-hydroxy-jolkinolide B;U251;cell apoptosis;Caspase-3;caspase-9
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发布时间：2024-09-23 - 摘要：Objective:To compare the analgesic effect and effective fractions of wild and cultivated Psammosilene tunicoides.Method:compounds of different polarity from roots of wild and cultivated P.tunicoides were extracted,hot plate test and acetic acid writhing test were used in mice for evaluating analgesic effect of the extracts.Result:The extracts of wild and cultivated P.tunicoides produced the similar analgesic effect;both of them contained highly active analgesic ingredients.For the inhibitory effect of the extracts on chemical stimulation induced pain,the active ingredients of wild and cultivated P.tunicoides were found in the parts of n-butanol,ethyl acetate and petroleum.But the ethyl acetate part of artificial cultivation sample had a significant analgesic activity better than that of the wild sample（P<0.05）.For the analgesic effect on thermal stimulation induced pain model,the effective fractions of wild P.tunicoides was the n-butanol part,the n-butanol and ethyl acetate parts were in cultivation sample.Conclusion:The artificial cultivation and wild herbs of P.tunicoides,haas the different active ingredients of analgesic function,but their analgesic efficacy do not show a significant difference.So the cultivated P.tunicoides could be used as the equivalent analgesic medicine to substitute the wild herbs.关键词：Psammosilene tunicoides;wild;cultivation;analgesia;effective fractions
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发布时间：2024-09-23 - 摘要：Objective: To study the hypotensive effect of Wuwei Jiangya Fang to spontaneous hypertensive rats(SHR), and analyze its blood exposure compositions which would be the effective components. Method: Wuwei Jiangya Fang was extracted with water and administrated to the SHR rats per oral, once a day; the dosage was 2.6 g · kg-1 per day. During the period of the experiment, the tail blood pressure was measured every week. SHR rats were anesthetized and the blood was taken from abdominal aorta. We established the fingerprints of Wuwei Jiangya Fang and its serum compounds with RP-HPLC method, and determined the exposed components which showed antihypertensive efficacy. Result: Wuwei Jiangya Fang showed obvious anti-hypertension effect (P<0.01) when the rats were administered for 2 weeks or longer. Through the analysis to blood multiple component fingerprint, we found that when Wuwei Jiangya Fang showed its antihypertensive effect, certain components which had UV absorption were found in the blood. Conclusion: Wuwei Jiangya Fang showed obvious anti-hypertension effect and certain exposure components of it which showed UV absorption were found in the blood. Further chemical research works were needed to clarify the character of the exposure components.关键词：exposure response;Wuwei Jiangya Fang;anti-hypertension;serum compounds
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发布时间：2024-09-23 - 摘要：Objective:To determine the content of tannins in above and under ground parts of scorzonera sinensis.To observe antioxidative activity of tannin from scorzonera sinensis.Method:The complexometric titration was used to determine the content of tannins.Iodometric and spectrophotometry were used to determine the effects of tannins on H2O2 and · OH.Result:The tannins in above and under ground parts of scorzonera sinensis was 8.18% and 9.27% accordingly.With the Concentration of 0.05 g · mL-1,the clearance rates on H2O2 and · OH were 8.89% and 88.24%.Conclusion:Tannins of scorzonera sinensis have a strong ability to resistance oxidation.
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- 170
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发布时间：2024-09-23 - 摘要：Objective: The evaluate the efficacy of the Hari Gabur-10 flavor tablets on digest. Method: The impact of the tablets (1.52,0.76,0.38 g ·kg-1)on pepsin activity and gastric secretion in rats was investigated by titration method and protein glass capillary method. The influence of the tablets (three doses) on gastric emptiness and intestinal propulsion was evaluated in mice through colorimetry and intestinal propulsion method. Result: Hari Gabur-10 flavored tablets could significantly promote gastric juice secretion in rats, increase gastric total acidity and pepsin activity;and accelerate the gastric emptying rate(P<0.05). It showed a certain role in propulsion of small intestine(P<0.05). Conclusion: Mongolian medicine Hari Gabur-10 flavor tablets has the effect of promoting digestion.关键词：Mongolian medicine;Hari gabur-10 flavor tablets;gastric juice;pepsin activity;gastrointestinal motility
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发布时间：2024-09-23 - 摘要：Objective:To evaluate the acute toxicity and the protective effects of Radix Adenophorae Potanini Korsh Polysaccharides dispersible tablets（RPDTS） on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.Method: The maximum dosage test was used as intragastric administration in mice to observe acute toxicity of RPDTS.After intragastric administration RPDTS（0.75,1.5,3 g · kg-1）in mice for 7 days,the acute liver injury model was induced by CCl4.The levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST） in serum and total superoxide dismutase（T-SOD）,malondialdehyde（MDA）,glutathione peroxideobjects enzyme（GSH-Px） in liver were determined by kits respectively.Histopathological examination was also carried out.Result: Acute toxicity test showed that the total dose of intragastric administration of RPDTS was 208 g · kg-1（according to Adenophorae tetraphylla） equivalent to 120 times the clinical dose for adults.Acute liver injury test showed that RPDTS could obviously decrease the levels of ALT,AST in serum and MDA in liver（P<0.05）,obviously increase the levels of T-SOD,GSH-Px in liver,the histopathological examination indicated that liver injuries were relieved.Conclusion: RPDTS has no acute toxicity,RPDTS has protective effects on acute liver injuries induced by CCl4 in mice.关键词：Radix Adenophorae Potanini Korsh Polysaccharides;dispersible tablets;acute toxicity;acute liver injury;CCl4;the maximum dosage test
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- 212
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发布时间：2024-09-23 - 摘要：Objective:To investigate the poctect function of Diyu Shengbai tabletts on bone marrow depression induced by cyclophosphamide in mice.Method: Twenty-four Balb/C mice,half male and femal,were categorized into three groups.Mice in model group and Diyu Shengbai group were administered cyclophosphamide（Cy）（100 mg · kg-1 once a day for 3 days by intraperitoneal injection） to induce marrow suppression.Diyu Shengbai group was received Diyu Shengbai table treatment（100 mg · kg-1body weight orally once a day for 10 days）.A vehicle treated control group was also arrangeed.The changes of peripheral hemogram,bone marrow nucleated cells（BMCs）,DNA content and bone marrow cell cycle were detected.Result:Cy-treated mice showed a significant decrease in the number of white blood cells,thrombocyte,bone marrow nucleated cell proliferation and the content of DNA in bone（compared with control group,there were significant differences,P<0.05 or P<0.01）.The percentage of G0/G1 phase was increased and the percentage G2/M phase decreased（compared with control group,there were significant differences,P<0.05 or P<0.01）.Diyu Shengbai tablets significantly increased the number of white blood cells,thrombocyte,bone marrow nucleated cell proliferation and markedly increased the percentage of G2/M phase(compared with model group（P<0.05 or P<0.01）.Conclusion:Diyu Shengbai Tablets could improve the hemopoietic function for marrow suppression induced by Cy in mice.关键词：Diyu Shengbai tablets;cyclophosphamide;bone marrow depression;cell cycle;bone marrow nucleated cells
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- 523
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- 34
发布时间：2024-09-23 - 摘要：Objective: To analysis chronic heart failure with blood stasis syndrome of different subtypes,to figure out the distribution of the drug of traditional Chinese medicine(TCM), so as to guide clinical drug use. Method: The retrospective analysis of 151 patients with chronic heart failure of combining traditional Chinese and western medicine treatment of patients with TCM diagnosis, syndrome type, heart function was classified. Result: The heart and blood stasis are the occurrence and development of important reason and the inevitable result, blood stasis syndrome types of heart failure for main (105 cases, 69.54%); and always with qi, yang deficiemcy.All patients with heart failure, heart function Ⅲ grade most see more, the different stages of the blood stasis subtypes distribution, blood stasis, different phlegm retention and mark the ups and downs of the empirical, this disease is repeated attacks of important reasons. Conclusion: Blood stasis syndrome is the most freguently occured syndrome with qi deficlency,yang deficiency,yin deficienty,phlegm stagnation etc. At different stages. In the treatment of the disease, the herbs for activating blood circulation and eliminating stasis appears to be the highest frequency, often used together with herbs of supplementing qi,alleviating water,warming yang and eliminating phlegm.关键词：chronic heart failure;blood stasis syndrome;evolution law;drug characteristics
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发布时间：2024-09-23 - 摘要：Objective:To study the effects of 17-hydroxy-jolkinolide B（HJB） on proliferation and apoptosis of U251 cells.Method:After the cells were treated with HJB at various concentrations（0,6.25,12.5,25,50,100,200,400,800 μmol·L-1）for 24 h,or at half maximal inhibitory concentration of a substance（IC50） for various durations（12,24,48,72 h）,cell viability was measured by 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）.Annexin V-FITC by cytometry was used to test the apoptosis rate.Absorption spectrometry was adopt to measure the relative activity of Caspase-3 and Caspase-9.Result:The HJB evidently inhibited the growth of U251 cells in a time and dose-dependent manner（P<0.05）,with IC50value for 24 h being 62.236 11 μmol · L-1,and it could induce apoptosis of U251 cells.After HJB treatment at 30,60 and 120 μmol · L-1for 24 h,the apoptosis rate of U251 cells was 3.10%,3.51% and 5.98%,respectively（P<0.05）,and the relative activity of Caspase-3 or Caspase-9 was advanced with dose-dependent manner.Conclusion:The HJB evidently inhibits the growth of U251 and induces the apoptosis of U251.The mechanism may be mediated by mitochondrion pathways.关键词：17-hydroxy-jolkinolide B;U251;cell apoptosis;Caspase-3;caspase-9
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- 137
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- 10
发布时间：2024-09-23 - 摘要：Objective: To observe Xuanfei Tongluo Pingchuan Decoction (XTPD) in treating attack stage of child asthma (cold and heat mixed type), to find effective traditional Chinese medicine for children asthma. Method: Children with asthma (cold and heat mixed type) of 60 cases were randomly divided into treatment group and control group (n=30,each). The treatment group was treated with XTPD except routine treatment. The treatment was carried out for 1 week. Result: The comparison of effect of the two groups:the total effective rate of the treatment group was 93.33%,the control group was 66.67%,the treatment group was better than the control group (P<0.05).The comparison of clinical effect of traditional Chinese medicine of the two groups,the total effective rate of the treatment group was 93.33%,the control group was 70.00%,the treatment group was better than the control group (P<0.05).The treatment group in the onset time of effetiveness, anti-asthma time, anti-coughing time,elimination of sputum was better than the control group (P<0.05). Conclusion: XTPD treatment was better than western medicine alone.关键词：attack stage of child asthma;cold and heat mixed type;Xuanfei Tongluo pgngchuan decoction
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- 0
发布时间：2024-09-23 - 摘要：Objective:To study the hypotensive effect of Wuwei Jiangya Fang to spontaneous hypertensive rats（SHR）,and analyze its blood exposure compositions which would be the effective components.Method: Wuwei Jiangya Fang was extracted with water and administrated to the SHR rats per oral,once a day;the dosage was 2.6 g · kg-1 per day.During the period of the experiment,the tail blood pressure was measured every week.SHR rats were anesthetized and the blood was taken from abdominal aorta.We established the fingerprints of Wuwei Jiangya Fang and its serum compounds with RP-HPLC method,and determined the exposed components which showed antihypertensive efficacy.Result: Wuwei Jiangya Fang showed obvious anti-hypertension effect（P<0.01） when the rats were administered for 2 weeks or longer.Through the analysis to blood multiple component fingerprint,we found that when Wuwei Jiangya Fang showed its antihypertensive effect,certain components which had UV absorption were found in the blood.Conclusion: Wuwei Jiangya Fang showed obvious anti-hypertension effect and certain exposure components of it which showed UV absorption were found in the blood.Further chemical research works were needed to clarify the character of the exposure components.关键词：exposure response;Wuwei Jiangya Fang;anti-hypertension;serum compounds
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- 152
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- 9
发布时间：2024-09-23 - 摘要：Objective: To study the clinical effect of traditional Chinese medicine combined with western medicine in treatment of liver cirrhosis ascites. Method: One handred and sixty patients with liver cirrhosis ascites from Mar, 2009 to Sep, 2011 were randomly divided into treatment group and control group. The patients in control group were treated with westeren medicine, the patients in treatment group was received with traditional Chinese medicine combined with western medicine. Three month after, clinical symptom, liver function, liver fibrosis and total effective ratio were compared. Result: After 3 months, the clinical symptom of treatment group was improved faster than that in control group(P<0.05);the functional marker of liver in treatment group was lower than that in control group(P<0.05). And liver fibrosis in treatment group was much optimistic than control group. And the abdominal circumference was less than control group(P<0.05);the total effective rate of treatment group was 90.0%, and the rate of control group was 68.8%(P<0.05). Conclusion: Traditional Chinese medicine combined with western medicine showed satisfactory clinical efficacy in the treatment of liver cirrhosis ascites. It improved clinical symptom significantly, live function and fibrosis of patients in treatment group were better those in than control group. and it was worthy of clinical use.关键词：traditional Chinese medicine;liver cirrhosis;ascites;clinical observation
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- 0
发布时间：2024-09-23 - 摘要：Objective:The evaluate the efficacy of the Hari Gabur-10 flavor tablets on digest.Method:The impact of the tablets（1.52,0.76,0.38 g·kg-1）on pepsin activity and gastric secretion in rats was investigated by titration method and protein glass capillary method.The influence of the tablets（three doses） on gastric emptiness and intestinal propulsion was evaluated in mice through colorimetry and intestinal propulsion method.Result:Hari Gabur-10 flavored tablets could significantly promote gastric juice secretion in rats,increase gastric total acidity and pepsin activity;and accelerate the gastric emptying rate（P<0.05）.It showed a certain role in propulsion of small intestine（P<0.05）.Conclusion:Mongolian medicine Hari Gabur-10 flavor tablets has the effect of promoting digestion.关键词：Mongolian medicine;Hari gabur-10 flavor tablets;gastric juice;pepsin activity;gastrointestinal motility
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- 236
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发布时间：2024-09-23 - 摘要：Objective: This study aimed to investigate the clinical efficacy of Shujin Tongluo granule (STG) in radicular type of cervical spondylosis and cervical spondylosis vertebral artery. Method: A retrospective analysis of the 172 outpatients and inpatients suffered from radicular type of cervical spondylosis and cervical spondylosis vertebral artery from May 2010 to May 2011 was carried out.Two types of cervical spondylosis were treated similarly,the control group was treated with conventional traction treatment,the massage therapy.Treatment group on the basis of the control group was given STG,1 bag/time,tid. The course of a month. Result: ①Compared the clinical efficacy of the two groups of radicular type of cervical spondylosis patients after treatment,the difference was statistically significant (treatment 85.45%,control 69.77%,P<0.05);②The main symptoms and signs disappeared with regard to the treatment group and control group revealed that pain,upper extremity numbness,limited neck mobility,tenderness,brachial plexus traction test,the test of top pressure difference were statistically significant (P<0.05). However,the upper limb muscle strength was no statistical differences.The treatment group,after treatment,reveals that pain,upper extremity numbness,limited Compared the clinical efficacy of the two groups of cervical spondylosis vertebral artery patients,the difference was statistically significant (treatment 92.86%,control 76.74%,P<0.05). The disappearance of main symptoms and signs with regard to the treatment group and control group revealed that vertigo,headache,the test of top pressure difference was significant(P<0.05),neck-rotation test was not significant. After treatment the diffence of vertigo,headache,the test of top pressure was statistically significant(P<0.05),but neck-rotation test showed no significantdifference. Conclusion: STG shows significant efficacy in radicular type of cervical spondylosis and cervical spondylosis vertebral artery formain symptoms and disappear ance of signs.关键词：Shujin Tongluo granule;cervical spondylosis;analysis of clinical efficacy
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- 0
发布时间：2024-09-23 - 摘要：目的:观察自拟补肾活血方桑仙降压颗粒剂对自发性高血压大鼠（SHR）心肌微血管密度（MVD）的干预,探讨其对微血管数目变化的影响。方法:SHR随机分为3组,模型对照组、吲哒帕胺（0.25 mg.kg-1）西药对照组、桑仙降压颗粒（含生药8 g.kg-1）组,以Wister大鼠为正常对照（蒸馏水）,每组6只,每周用药6 d,1次/d,4周及8周后观察SHR心肌MVD的表达。结果:与模型组比较,4周及8周后桑仙降压颗粒组标记心肌MVD的CD34表达均明显增多。结论:桑仙降压颗粒能有效减轻SHR大鼠心肌微血管损害。关键词：桑仙降压颗粒;自发性高血压大鼠;心肌微血管密度;CD34
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发布时间：2024-09-23 - 摘要：Objective: To observe effect of Huoxue Tiaozhi Decoction on blood rheology of blood lipid regulation in coronary heart disease patients after percutaneous coronary intervention (PCI). Method: With incorporated into standard in 60 cases of PCI patients were randomly divided into observation group and control group,40 cases in each. Two groups of patients after PCI were given aspirin enteric-coated tablets 100 mg,bid, simvastatin tablet:(40 mg,bid).Observation group was added with Huoxue Tiaozhi Decoction, boiling water service,250 mL/bid. The 2 groups were treated for 1 month as 1 course,2 courses as the treatment.Hemorheology and serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) were observed. Result: The blood high, medium, low shear viscosity, plasma viscosity, hematocrit in treatment group were (3.52±0.62), (4.33±0.54), (7.18±1.55), (1.32±0.14) mPa · s, (34.43±5.54)%;the control group were (3.72±0.58), (4.52±0.64), (7.41±1.23), (1.41± 0.15) mPa · s, (36.78±5.68)%. The two groups after treatment were significantly improved (P<0.05), and the treat ment group was better than the control group (P<0.05). TC, TG, LDL-C, HDL-C indexes after treatment in treatment group were (3.61±0.41), (2.31±0.52), (3.75±0.38), (1.95±0.26) mmol · L-1, those in the control group were (3.72±0.58), (4.52±0.64), (7.41±1.23), (1.41±0.15) mmol · L-1. The two groups after treatment were significantly improved (P<0.05), and the treatment group was better than the control group (P<0.05). Conclusion: Huoxue Tiaozhi decoction used in coronary heart disease patients after PCI can improve hemorheological paramenters, blood lipids, reduce the incidence of restenosis after PCI.关键词：coronary heart disease;PCI;Huoxue Tiaozhi decoction;hemorheology;blood lipid
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发布时间：2024-09-23 - 摘要：Objective:To analysis chronic heart failure with blood stasis syndrome of different subtypes,to figure out the distribution of the drug of traditional Chinese medicine（TCM）,so as to guide clinical drug use.Method:The retrospective analysis of 151 patients with chronic heart failure of combining traditional Chinese and western medicine treatment of patients with TCM diagnosis,syndrome type,heart function was classified.Result:The heart and blood stasis are the occurrence and development of important reason and the inevitable result,blood stasis syndrome types of heart failure for main（105 cases,69.54%）;and always with qi,yang deficiemcy.All patients with heart failure,heart function Ⅲ grade most see more,the different stages of the blood stasis subtypes distribution,blood stasis,different phlegm retention and mark the ups and downs of the empirical,this disease is repeated attacks of important reasons.Conclusion:Blood stasis syndrome is the most freguently occured syndrome with qi deficlency,yang deficiency,yin deficienty,phlegm stagnation etc.At different stages.In the treatment of the disease,the herbs for activating blood circulation and eliminating stasis appears to be the highest frequency,often used together with herbs of supplementing qi,alleviating water,warming yang and eliminating phlegm.关键词：chronic heart failure;blood stasis syndrome;evolution law;drug characteristics
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- 156
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- 14
发布时间：2024-09-23 - 摘要：Objective: To investigate the Integrative Medicine clinical efficacy of treatment with stationary phase chronic obstructive pulmonary disease (COPD) with pulmonary heart disease. Method: A clear diagnosis of stationary phase COPD with pulmonary heart disease, 100 patients were randomly divided into control group of 50 cases and the observation group of 50 cases, the control group only received the formal Western medical treatment;the observation group in the control group treatment program combined with traditional Chinese medicine theory, with traditional Chinese medicine to carry out comprehensive treatment. Both groups were at least 4 weeks of treatment to be evaluated. Result: After 4 weeks of treatment, the observation group, whether the efficacy of chronic pulmonary heart disease (total efficiency of 94%) or respiratory symptoms improved (total effective rate of 82%) are significantly better than the control group only to Western medicine treatment (total efficiency of 76% and 74% respectively, P<0.01 or P<0.05). In addition, the SGRQ score showed that the patients by the integrative medicine has been markedly improved quality of life of the observation group than before treatment (P<0.01). Conclusion: Integrative treatment of COPD with pulmonary heart disease have obvious advantages, can effectively increase the clinical efficacy of the disease, improve the quality of life of patients.关键词：stationary phase;COPD;pulmonary heart disease;Integrative Medicine;clinical observation
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发布时间：2024-09-23 - 摘要：Objective:To observe Xuanfei Tongluo Pingchuan Decoction（XTPD） in treating attack stage of child asthma（cold and heat mixed type）,to find effective traditional Chinese medicine for children asthma.Method:Children with asthma（cold and heat mixed type） of 60 cases were randomly divided into treatment group and control group（n=30,each）.The treatment group was treated with XTPD except routine treatment.The treatment was carried out for 1 week.Result:The comparison of effect of the two groups:the total effective rate of the treatment group was 93.33%,the control group was 66.67%,the treatment group was better than the control group（P<0.05）.The comparison of clinical effect of traditional Chinese medicine of the two groups,the total effective rate of the treatment group was 93.33%,the control group was 70.00%,the treatment group was better than the control group（P<0.05）.The treatment group in the onset time of effetiveness,anti-asthma time,anti-coughing time,elimination of sputum was better than the control group（P<0.05）.Conclusion:XTPD treatment was better than western medicine alone.关键词：attack stage of child asthma;cold and heat mixed type;Xuanfei Tongluo pgngchuan decoction
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- 177
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- 23
发布时间：2024-09-23 - 摘要：Objective: To evaluate the effect of Levam Lodipine and Salvia Miltiorrhiza Freeze-dried powder injection combination on in-stentrestenosis after intracoronary stenting. Method: Two hundreds and Twenty-two coronary heart disease patients with successful intracoronary stenting were divided randomly into the control group, the group of Salvia Miltiorrhiza Freeze-dried powder injection,the group of Levam Lodipine and the group of combination (Levam Lodipine plus Salvia Miltiorrhiza Freeze-dried powder injection). Coronary angiograhpic(CAG) was made in patients with the angiograms of post-intervention and 6 months after treatment for all group. And serum(endothelin) ET, (nitrogen monoxide)NO, (triglycerid)TG and(serum total cholestero)TC were detected in patients with the angiograms of post-intervention and 6 months after treatment for all group. Result: In-stent restenosis (ISR) rate was significant decreased in the group of combination(P<0.05). The serum TC and TG levels were decreased in the group of Salvia Miltiorrhiza Freeze-dried powder injection, the group of Levam Lodipine and the group of combination. The serum ET level was decreased in the group of Levam Lodipine and the group of combination, while the NO levels was increased(P<0.05). Conclusion: Both Salvia Miltiorrhiza Freeze-dried powder injection and Levam Lodipine has benefit for preventing restenosis after intracoronary stenting.关键词：Levam Lodipine;Salvia Miltiorrhiza Freeze-dried Powder injection;in-stentrestenosis after intracoronary stenting
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发布时间：2024-09-23 - 摘要：Objective:To study the clinical effect of traditional Chinese medicine combined with western medicine in treatment of liver cirrhosis ascites.Method:One handred and sixty patients with liver cirrhosis ascites from Mar,2009 to Sep,2011 were randomly divided into treatment group and control group.The patients in control group were treated with westeren medicine,the patients in treatment group was received with traditional Chinese medicine combined with western medicine.Three month after,clinical symptom,liver function,liver fibrosis and total effective ratio were compared.Result:After 3 months,the clinical symptom of treatment group was improved faster than that in control group（P<0.05）;the functional marker of liver in treatment group was lower than that in control group（P<0.05）.And liver fibrosis in treatment group was much optimistic than control group.And the abdominal circumference was less than control group（P<0.05）;the total effective rate of treatment group was 90.0%,and the rate of control group was 68.8%（P<0.05）.Conclusion:Traditional Chinese medicine combined with western medicine showed satisfactory clinical efficacy in the treatment of liver cirrhosis ascites.It improved clinical symptom significantly,live function and fibrosis of patients in treatment group were better those in than control group.and it was worthy of clinical use.关键词：traditional Chinese medicine;liver cirrhosis;ascites;clinical observation
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- 312
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- 30
发布时间：2024-09-23 - 摘要：Objective: To explore effect of combination of Aidi injection and neoadjuvant chemotherapy on patients with non-small-cell lung cancer and number of CD4+CD25+ T regulatory cells (NSCLC). Method: Fifty patients with NSCLC were chosen for this research. Patients were divided into control group of 20 patients without application of Aidi injection and treatment group of 35 patients applied with Aidi injection. The following indexes like their short-term curative effect, operation time, blood loss in operations, ablation rate and side effects were observed;flow cytometry was used to detect and compare number of Treg before and after treatments. Result: Compared with control group, blood loss in operation was decreased significantly (615.49±114.08)mL vs.(530.15±104.55)mL (P<0.05), curative effect increased significantly (30.00% vs 45.71%) (P<0.05), and occurrence of leucopenia decreased significantly (60.00% vs 37.14%) (P<0.05). Number of Treg was decreased significantly after treatments for all patients (P<0.05),which was more significant in treatment group than in control group (P<0.05). Conclusion: Combination of Aidi injection and neoadjuvant chemotherapy can enhance clinical effects on patients with NSCLC, meanwhile enhance tumor immunity and reduce side effect during the treatment.关键词：Aidi injection;neoadjuvant chemotherapy;non-small-cell lung cancer;tumor immunity
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发布时间：2024-09-23 - 摘要：Objective:This study aimed to investigate the clinical efficacy of Shujin Tongluo granule（STG） in radicular type of cervical spondylosis and cervical spondylosis vertebral artery.Method:A retrospective analysis of the 172 outpatients and inpatients suffered from radicular type of cervical spondylosis and cervical spondylosis vertebral artery from May 2010 to May 2011 was carried out.Two types of cervical spondylosis were treated similarly,the control group was treated with conventional traction treatment,the massage therapy.Treatment group on the basis of the control group was given STG,1 bag/time,tid.The course of a month.Result:①Compared the clinical efficacy of the two groups of radicular type of cervical spondylosis patients after treatment,the difference was statistically significant（treatment 85.45%,control 69.77%,P<0.05）;②The main symptoms and signs disappeared with regard to the treatment group and control group revealed that pain,upper extremity numbness,limited neck mobility,tenderness,brachial plexus traction test,the test of top pressure difference were statistically significant（P<0.05）.However,the upper limb muscle strength was no statistical differences.The treatment group,after treatment,reveals that pain,upper extremity numbness,limited Compared the clinical efficacy of the two groups of cervical spondylosis vertebral artery patients,the difference was statistically significant（treatment 92.86%,control 76.74%,P<0.05）.The disappearance of main symptoms and signs with regard to the treatment group and control group revealed that vertigo,headache,the test of top pressure difference was significant（P<0.05）,neck-rotation test was not significant.After treatment the diffence of vertigo,headache,the test of top pressure was statistically significant（P<0.05）,but neck-rotation test showed no significantdifference.Conclusion:STG shows significant efficacy in radicular type of cervical spondylosis and cervical spondylosis vertebral artery formain symptoms and disappear ance of signs.关键词：Shujin Tongluo granule;cervical spondylosis;analysis of clinical efficacy
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- 197
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发布时间：2024-09-23 - 摘要：Objective: To observe the effect of Huomai powder on thromboangiitis obliterans. Method: Ninety subjects who suffered from thromboangiitis obliterans with syndrome of blood stasis were divided into three groups:treatment group(30 cases),given routine treatment of western medicine and Huomai powder;control group 1(30 cases),given Routine treatment of western medicine and Xuefu Zhuyu capsule;control group 2 (30 cases),given routine treatment of western medicine. Result: In the clinical efficacy, the treatment group was better than the control group(P<0.05). The treatment group before and after treatment with ABI, FIB had significant difference(P<0.05). Conclusion: The treatment group was more effective than control group. Huomai powder was effective in treating thromboangiitis obliterans with syndrome of blood stasis.关键词：Huomai powder;thromboangiitis obliterans;blood stasis
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发布时间：2024-09-23 - 摘要：Objective:To observe effect of Huoxue Tiaozhi Decoction on blood rheology of blood lipid regulation in coronary heart disease patients after percutaneous coronary intervention（PCI）.Method:With incorporated into standard in 60 cases of PCI patients were randomly divided into observation group and control group,40 cases in each.Two groups of patients after PCI were given aspirin enteric-coated tablets 100 mg,bid,simvastatin tablet:（40 mg,bid）.Observation group was added with Huoxue Tiaozhi Decoction,boiling water service,250 mL/bid.The 2 groups were treated for 1 month as 1 course,2 courses as the treatment.Hemorheology and serum total cholesterol（TC）,triglyceride（TG）,low density lipoprotein（LDL-C）,high density lipoprotein（HDL-C） were observed.Result:The blood high,medium,low shear viscosity,plasma viscosity,hematocrit in treatment group were（3.52±0.62）,（4.33±0.54）,（7.18±1.55）,（1.32±0.14） mPa · s,（34.43±5.54）%;the control group were（3.72±0.58）,（4.52±0.64）,（7.41±1.23）,（1.41± 0.15） mPa · s,（36.78±5.68）%.The two groups after treatment were significantly improved（P<0.05）,and the treat ment group was better than the control group（P<0.05）.TC,TG,LDL-C,HDL-C indexes after treatment in treatment group were（3.61±0.41）,（2.31±0.52）,（3.75±0.38）,（1.95±0.26） mmol · L-1,those in the control group were（3.72±0.58）,（4.52±0.64）,（7.41±1.23）,（1.41±0.15） mmol · L-1.The two groups after treatment were significantly improved（P<0.05）,and the treatment group was better than the control group（P<0.05）.Conclusion:Huoxue Tiaozhi decoction used in coronary heart disease patients after PCI can improve hemorheological paramenters,blood lipids,reduce the incidence of restenosis after PCI.关键词：coronary heart disease;PCI;Huoxue Tiaozhi decoction;hemorheology;blood lipid
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- 66
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- 4
发布时间：2024-09-23 - 摘要：Objective: To observe the clinical efficacy of sugar-free particle of Rhodobryum giganteum in treatment angina of diabetic patients with coronary heart disease. Method: A randomized double-blind method was used, 105 patients were divided into two groups, and respectively treated with Huixin grass sugar-free particles (66 cases in treatment group) and musk heart-protecting pill (36 patients in control group), the change was observed on angina pain, electrocardiograph(ECG), stop or reduction rate nitroglycerin. Result: The efficiency of treatment group was 92.4% in curing angina and 89.7% in control group. There was no significant difference between treatment and control groups. Electrocardiogram efficiency in the treatment group was 57.6% and the control group was 43.6%(P<0.05).Reduction rate of nitroglycerin was 83.3% in treatment group and the control group was 79.5%. Conclusion: The sugar-free particle of R. giganteum of angina pectoris has a good effect in treating angina of diabetic patients with coronary heart disease (ECG improved more significantly).关键词：sugar-free particle of Rhodobryum giganteum;coronary heart disease;angina
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To investigate the Integrative Medicine clinical efficacy of treatment with stationary phase chronic obstructive pulmonary disease（COPD） with pulmonary heart disease.Method:A clear diagnosis of stationary phase COPD with pulmonary heart disease,100 patients were randomly divided into control group of 50 cases and the observation group of 50 cases,the control group only received the formal Western medical treatment;the observation group in the control group treatment program combined with traditional Chinese medicine theory,with traditional Chinese medicine to carry out comprehensive treatment.Both groups were at least 4 weeks of treatment to be evaluated.Result:After 4 weeks of treatment,the observation group,whether the efficacy of chronic pulmonary heart disease（total efficiency of 94%） or respiratory symptoms improved（total effective rate of 82%） are significantly better than the control group only to Western medicine treatment（total efficiency of 76% and 74% respectively,P<0.01 or P<0.05）.In addition,the SGRQ score showed that the patients by the integrative medicine has been markedly improved quality of life of the observation group than before treatment（P<0.01）.Conclusion:Integrative treatment of COPD with pulmonary heart disease have obvious advantages,can effectively increase the clinical efficacy of the disease,improve the quality of life of patients.关键词：stationary phase;COPD;pulmonary heart disease;Integrative Medicine;clinical observation
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- 147
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- 18
发布时间：2024-09-23 - 摘要：Objective:To evaluate the effect of Levam Lodipine and Salvia Miltiorrhiza Freeze-dried powder injection combination on in-stentrestenosis after intracoronary stenting.Method:Two hundreds and Twenty-two coronary heart disease patients with successful intracoronary stenting were divided randomly into the control group,the group of Salvia Miltiorrhiza Freeze-dried powder injection,the group of Levam Lodipine and the group of combination（Levam Lodipine plus Salvia Miltiorrhiza Freeze-dried powder injection）.Coronary angiograhpic（CAG） was made in patients with the angiograms of post-intervention and 6 months after treatment for all group.And serum（endothelin） ET,（nitrogen monoxide）NO,（triglycerid）TG and（serum total cholestero）TC were detected in patients with the angiograms of post-intervention and 6 months after treatment for all group.Result:In-stent restenosis（ISR） rate was significant decreased in the group of combination（P<0.05）.The serum TC and TG levels were decreased in the group of Salvia Miltiorrhiza Freeze-dried powder injection,the group of Levam Lodipine and the group of combination.The serum ET level was decreased in the group of Levam Lodipine and the group of combination,while the NO levels was increased（P<0.05）.Conclusion:Both Salvia Miltiorrhiza Freeze-dried powder injection and Levam Lodipine has benefit for preventing restenosis after intracoronary stenting.关键词：Levam Lodipine;Salvia Miltiorrhiza Freeze-dried Powder injection;in-stentrestenosis after intracoronary stenting
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- 67
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- 5
发布时间：2024-09-23 - 摘要：Objective:To explore effect of combination of Aidi injection and neoadjuvant chemotherapy on patients with non-small-cell lung cancer and number of CD4+CD25+ T regulatory cells（NSCLC）.Method:Fifty patients with NSCLC were chosen for this research.Patients were divided into control group of 20 patients without application of Aidi injection and treatment group of 35 patients applied with Aidi injection.The following indexes like their short-term curative effect,operation time,blood loss in operations,ablation rate and side effects were observed;flow cytometry was used to detect and compare number of Treg before and after treatments.Result:Compared with control group,blood loss in operation was decreased significantly（615.49±114.08）mL vs.（530.15±104.55）mL（P<0.05）,curative effect increased significantly（30.00% vs 45.71%）（P<0.05）,and occurrence of leucopenia decreased significantly（60.00% vs 37.14%）（P<0.05）.Number of Treg was decreased significantly after treatments for all patients（P<0.05）,which was more significant in treatment group than in control group（P<0.05）.Conclusion:Combination of Aidi injection and neoadjuvant chemotherapy can enhance clinical effects on patients with NSCLC,meanwhile enhance tumor immunity and reduce side effect during the treatment.关键词：Aidi injection;neoadjuvant chemotherapy;non-small-cell lung cancer;tumor immunity
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- 133
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- 11
发布时间：2024-09-23 - 摘要：Objective:To observe the effect of Huomai powder on thromboangiitis obliterans.Method:Ninety subjects who suffered from thromboangiitis obliterans with syndrome of blood stasis were divided into three groups:treatment group（30 cases）,given routine treatment of western medicine and Huomai powder;control group 1（30 cases）,given Routine treatment of western medicine and Xuefu Zhuyu capsule;control group 2（30 cases）,given routine treatment of western medicine.Result:In the clinical efficacy,the treatment group was better than the control group（P<0.05）.The treatment group before and after treatment with ABI,FIB had significant difference（P<0.05）.Conclusion:The treatment group was more effective than control group.Huomai powder was effective in treating thromboangiitis obliterans with syndrome of blood stasis.关键词：Huomai powder;thromboangiitis obliterans;blood stasis
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- 151
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- 9
发布时间：2024-09-23 - 摘要：Objective: To analyze application of Chinese herbal injections in our hospital, and provide reference for clinical rational application. Method: The amount of medication, medication frequency defined daily dose(DDDs), daily expense DDC and the amount of drug scheduling and DDDs sort ratio were given as the evaluation indexes, the application status of Chinese herbal injection was analyzed in 2011. Result: Fourty-two chemicals regulation of 34 varieties of Chinese herbal injection were used, highest frequency is xingnaojing injection, Chinese herbal withpromoting blood circulation and removing blood stasis is in key position. Conclusion: Application of Chinese herbal is reasonable, but there are still weakness,to which more attention should be paid.关键词：Chinese herbal injection;amount of medication;DDDs;daily cost;order ratio
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To observe the clinical efficacy of sugar-free particle of Rhodobryum giganteum in treatment angina of diabetic patients with coronary heart disease.Method: A randomized double-blind method was used,105 patients were divided into two groups,and respectively treated with Huixin grass sugar-free particles（66 cases in treatment group） and musk heart-protecting pill（36 patients in control group）,the change was observed on angina pain,electrocardiograph（ECG）,stop or reduction rate nitroglycerin.Result:The efficiency of treatment group was 92.4% in curing angina and 89.7% in control group.There was no significant difference between treatment and control groups.Electrocardiogram efficiency in the treatment group was 57.6% and the control group was 43.6%（P<0.05）.Reduction rate of nitroglycerin was 83.3% in treatment group and the control group was 79.5%.Conclusion:The sugar-free particle of R.giganteum of angina pectoris has a good effect in treating angina of diabetic patients with coronary heart disease（ECG improved more significantly）.关键词：sugar-free particle of Rhodobryum giganteum;coronary heart disease;angina
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- 99
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- 2
发布时间：2024-09-23 - 摘要：目的:探讨灯盏花素联合低分子肝素钙治疗急性脑梗死的疗效及安全性。方法:将急性脑梗死患者132例随机分为治疗组（66例）和对照组（66例）,在两组患者均给予急性脑梗死常规治疗的基础上,对照组给予灯盏花素;治疗组给予灯盏花素联合低分子肝素钙。观察两组患者NIHSS评分、日常生活能力（Barthel指数）评分及血液流变学指标变化,并随时记录用药不良反应。结果:两组患者治疗后的NIHSS评分较治疗前明显降低,Barthel指数评分明显升高,且治疗组的改善比对照组更明显（P<0.05）,治疗组总有效率为90.9%,对照组为77.3%（P<0.05）。治疗组未发现明显不良反应。结论:灯盏花素联合低分子肝素钙治疗急性脑梗死能明显改善患者的神经功能、日常生活能力及血液流变学指标,且安全有效。关键词：灯盏花素;低分子肝素钙;急性脑梗死
- 0
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- 70
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- 8
发布时间：2024-09-23 - 摘要：目的:探讨中药联合促性腺激素释放激素治疗子宫内膜异位症的疗效。方法:将诊断为子宫内膜异位症的240例患者随机分为对照组和中西医联合治疗组,对照组应用促性腺激素释放激素亮丙瑞林,0.375 mg.kg-1,1次/月,sc共6次);中西药联合治疗组在对照治疗的基础上,加用中药汤剂内服和肛门灌肠。比较两组治疗期间的围绝经期症状严重程度、两组的治疗效果及停药后复发情况。结果:治疗组用药期间围绝经期症状轻或无症状,对照组症状加重,Kupperman评分治疗组明显低于对照组。治疗组显效率为74.17%,复发率15%;对照组显效率为33.33%,复发率81.67%,两组比较差异有统计学意义（P<0.05）。结论:中西药结合治疗子宫内膜异位症具有疗效好,副作用小,复发率低的特点。关键词：促性腺激素释放激素;子宫内膜异位症;围绝经期症状
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- 86
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- 10
发布时间：2024-09-23 - 摘要：目的:观察自拟降糖方治疗2型糖尿病（痰瘀互结型）的临床疗效及血糖、血脂、血液流变指标的变化。方法:76例随机分为治疗组和对照组各38例。两组均予糖尿病饮食、适当运动等基础治疗,并口服二甲双胍、阿卡波糖,治疗组加服自拟降糖方,疗程均为30 d。比较两组中医证候变化,血糖（FPG）,2 h糖耐量（2 hPG）、血脂、血液流变之指标变化。结果:治疗组总有效率为97.37%、对照组总有效率为89.47%,两组比较差异有统计学意义（P<0.05）。治疗组治疗后2 hPG,FPG,血清总胆固醇TC,低密度脂蛋白胆固醇（LDL-C）,血液流变学指标均明显下降（P<0.05）。结论:自拟降糖方治疗2型糖尿病（痰瘀互结型）明显改善症状、调节血脂、血糖,改善血液流变性等,疗效优于单纯西药组。关键词：2型糖尿病;自拟降糖方;痰瘀互结
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- 139
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- 6
发布时间：2024-09-23 - 摘要：To analyze and review on the literature about the research of traditional Chinese medicine that acting on calcium signal transduction pathways of myocardial cell. So far, more and more traditional Chinese medicine used in the prevention and treatment of clinical cardiovascular arrhythmia disease, and the method which to combine calcium ion channels and related receptor to research the mechanism of anti-arrhythmic drugs has become a hot spot. 21 latest and representative references was searched and chosen from the MEDLINE and CNKI database using calcium channel, traditional Chinese medicine and patch clamp technique etc. as keywords, then summarizes the new progress on the research of traditional Chinese medicine that acting on calcium signal transduction pathways of myocardial cell. This paper reviews lately research status about effects of Chinese herbs on calcium channels and related protein receptors of calcium signal transduction pathways of myocardial cells, and states some problems existing in current research, and looks ahead the future study to provide new ideas and experimental basis.关键词：Myocardial cells;Calcium channel;signal transduction pathway;patch clamp technique
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- 0
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- 0
发布时间：2024-09-23 - 摘要：Objective:To analyze application of Chinese herbal injections in our hospital,and provide reference for clinical rational application.Method:The amount of medication,medication frequency defined daily dose（DDDs）,daily expense DDC and the amount of drug scheduling and DDDs sort ratio were given as the evaluation indexes,the application status of Chinese herbal injection was analyzed in 2011.Result:Fourty-two chemicals regulation of 34 varieties of Chinese herbal injection were used,highest frequency is xingnaojing injection,Chinese herbal withpromoting blood circulation and removing blood stasis is in key position.Conclusion:Application of Chinese herbal is reasonable,but there are still weakness,to which more attention should be paid.关键词：Chinese herbal injection;amount of medication;DDDs;daily cost;order ratio
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- 104
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- 5
发布时间：2024-09-23 - 摘要：This paper revieed the progress on the researches and applications of microfluidics in medical field. We summarized the history of microfluidics and introduced its application in medicine quality control, clinical trial and pharmacological research. The characteristics of microfluidics such as rapid, miniature, automatic, low consumption and high efficiency have been effectively reflected, while, there still need to be improved in the aspects of precision, accuracy and commercialization. The development prospect of microfluidics in drug discovery and traditional Chinese medicine quality control was also discussed. Microfluidics applied in the medical field is still in the primary stage, there are still some problems need to be solved. This review expects to provide reference for the further development of microfluidics in medical field.关键词：microfluidics;quality control;clinical testing;pharmacological research;drug discovery
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:观察红花逍遥片治疗围绝经期综合征的临床疗效。方法:将67例围绝经期综合征患者随机分为两组,观察组35人应用红花逍遥片（1.56 g,3次/d）治疗,对照组32人应用逍遥丸（9 g,3次/d）治疗。20 d1个疗程,5个疗程后观察两组症状积分、临床疗效、及血清促卵泡生成素（FSH）、黄体生成素（LH）、雌二醇（E2）含量。结果:观察组症状改善总有效率94.3%,优于对照组78.2%（P<0.05）;观察组临床疗效总有效率91.4%,优于对照组71.9%（P<0.05）;治疗后观察组FSH,LH,E2分别为[（18.3±6.17）,（16.5±4.2）U.L-1],（80.6±18.3）ng.L-1,对照组为[（25.9±7.2）,（20.3±3.5）U.L-1],（50.7±15.6）ng.L-1。观察组降低血清FSH,LH含量,升高血清E2含量疗效明显优于对照组（P<0.01）。结论:红花逍遥片治疗围绝经期综合征疗效显著。关键词：红花逍遥片;围绝经期综合征;临床研究
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- 196
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- 14
发布时间：2024-09-23 - 摘要：To summarize the research process of universal herbal pair containing aconitine in recent years, including clinical application, chemical research, pharmacological and toxicological research, apply theoretical bases of the aconite root species safely and effectively used in clinical application. Literatures on universal herbal pair containing aconitine in recent years were summarized and analyzed. Suitable compatibility of Aconitine root species can decrease toxicity and increase efficacy, enlarge applied range. There are some advancements in studies on reducing the toxicity and increasing efficacy of aconitine by using compatibility at present, but many researches are superficial, so it is necessary for us to further study the mechanism of detoxification and synergism by using compatibility, in order to apply theoretical bases of clinical application.关键词：Radix Aconiti;Radix Aconite Laterlis Prepapata;compatibility;decreasing toxicity
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:观察疏肝健脾和胃方治疗肝郁脾虚型慢性胃炎的临床疗效。方法:将175例慢性胃炎患者按随机数字表法分为治疗组（87例）、对照组（88例）。治疗组服用疏肝健脾和胃方,每日1剂,水煎,早晚分服。对照组口服奥美拉唑肠溶胶囊、多潘立酮片、硫糖铝片、阿莫西林胶囊治疗。30 d为1个疗程。结果:治疗组总有效率为86.90%;对照组总有效率为73.33%,两组比较差异显著（P<0.05）;两组幽门螺杆菌清除率、胃黏膜炎症改善率比较均有统计学差异,治疗组优于对照组（P<0.05）。结论:疏肝健脾和胃方治疗肝郁脾虚型慢性胃炎有较好的临床疗效,且安全性较高。关键词：疏肝健脾和胃方;慢性胃炎;肝郁脾虚证
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- 162
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- 36
发布时间：2024-09-23 - 摘要：To explore the characteristics of era, preparation and drugs of the formulas which contain the incompatible Radix et Rhizoma Veratri Nigri pairs of the '18 forbidden incompatible drugs’. One hundred formulas containing Radix et Rhizoma Veratri Nigri pairs were selected out of 96 429 formulas;association rules and correspondence analysis were applied to the 100 formulas to investigate the above-mentioned features. The formulas containing Radix et Rhizoma Veratri Nigri pairs were usually combined with Radix et Rhizoma Asari, Radix Aconiti Lateralis Praeparata, Realgar, Radix Sophorae Flavescentis, Rhizoma Chuanxiong, Radix et Rhizoma Ginseng, etc;and powder was the most common preparation for external use. The formulas containing Radix et Rhizoma Veratri Nigri pairs were most frequently used in Song Dynasty in powder;they lay heavy emphasis on the combination with drugs for warming yang qi, drying dampness to resolve phlegm, and activating qi and blood circulation.关键词：the 18 incompatible pairs of drugs;the ancient formulas;Radix et Rhizoma Veratri Nigri;data mining
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- 0
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- 0
发布时间：2024-09-23 - 摘要：目的:通过临床观察评价栀子豉汤对抑郁证的临床作用。方法:采用随机、对照原则,将患者分成栀子豉汤治疗组44例剂量,盐酸氟西汀对照组46例,15 d为1个疗程,3个疗程结束后,对患者汉密尔顿（HAMD,24项）积分、Kupperman（KMI）指数、中医症候积分的变化做统计学处理,评价栀子豉汤治疗郁证的临床疗效。结果:在改善某些症状体征方面与盐酸氟西汀疗效相当,在对全身症状的综合改善上优于盐酸氟西汀,副作用比盐酸氟西汀少。结论:本论文为栀子豉汤治疗郁证的临床应用可行性提供理论依据。关键词：栀子豉汤;郁证;临床观察
- 0
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- 440
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- 22
发布时间：2024-09-23 - 摘要：To analyze and review on the literature about the research of traditional Chinese medicine that acting on calcium signal transduction pathways of myocardial cell.So far,more and more traditional Chinese medicine used in the prevention and treatment of clinical cardiovascular arrhythmia disease,and the method which to combine calcium ion channels and related receptor to research the mechanism of anti-arrhythmic drugs has become a hot spot.21 latest and representative references was searched and chosen from the MEDLINE and CNKI database using calcium channel,traditional Chinese medicine and patch clamp technique etc.as keywords,then summarizes the new progress on the research of traditional Chinese medicine that acting on calcium signal transduction pathways of myocardial cell.This paper reviews lately research status about effects of Chinese herbs on calcium channels and related protein receptors of calcium signal transduction pathways of myocardial cells,and states some problems existing in current research,and looks ahead the future study to provide new ideas and experimental basis.关键词：Myocardial cells;Calcium channel;signal transduction pathway;patch clamp technique
- 0
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- 557
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- 9
发布时间：2024-09-23 - 摘要：This paper revieed the progress on the researches and applications of microfluidics in medical field.We summarized the history of microfluidics and introduced its application in medicine quality control,clinical trial and pharmacological research.The characteristics of microfluidics such as rapid,miniature,automatic,low consumption and high efficiency have been effectively reflected,while,there still need to be improved in the aspects of precision,accuracy and commercialization.The development prospect of microfluidics in drug discovery and traditional Chinese medicine quality control was also discussed.Microfluidics applied in the medical field is still in the primary stage,there are still some problems need to be solved.This review expects to provide reference for the further development of microfluidics in medical field.关键词：microfluidics;quality control;clinical testing;pharmacological research;drug discovery
- 0
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- 1192
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- 11
发布时间：2024-09-23 - 摘要：To summarize the research process of universal herbal pair containing aconitine in recent years,including clinical application,chemical research,pharmacological and toxicological research,apply theoretical bases of the aconite root species safely and effectively used in clinical application.Literatures on universal herbal pair containing aconitine in recent years were summarized and analyzed.Suitable compatibility of Aconitine root species can decrease toxicity and increase efficacy,enlarge applied range.There are some advancements in studies on reducing the toxicity and increasing efficacy of aconitine by using compatibility at present,but many researches are superficial,so it is necessary for us to further study the mechanism of detoxification and synergism by using compatibility,in order to apply theoretical bases of clinical application.关键词：Radix Aconiti;Radix Aconite Laterlis Prepapata;compatibility;decreasing toxicity
- 0
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- 1276
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- 37
发布时间：2024-09-23 - 摘要：To explore the characteristics of era,preparation and drugs of the formulas which contain the incompatible Radix et Rhizoma Veratri Nigri pairs of the ’18 forbidden incompatible drugs’.One hundred formulas containing Radix et Rhizoma Veratri Nigri pairs were selected out of 96 429 formulas;association rules and correspondence analysis were applied to the 100 formulas to investigate the above-mentioned features.The formulas containing Radix et Rhizoma Veratri Nigri pairs were usually combined with Radix et Rhizoma Asari,Radix Aconiti Lateralis Praeparata,Realgar,Radix Sophorae Flavescentis,Rhizoma Chuanxiong,Radix et Rhizoma Ginseng,etc;and powder was the most common preparation for external use.The formulas containing Radix et Rhizoma Veratri Nigri pairs were most frequently used in Song Dynasty in powder;they lay heavy emphasis on the combination with drugs for warming yang qi,drying dampness to resolve phlegm,and activating qi and blood circulation.关键词：the 18 incompatible pairs of drugs;the ancient formulas;Radix et Rhizoma Veratri Nigri;data mining
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- 331
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发布时间：2024-09-23 - 摘要：目的:探讨慢性湿疹的中医用药规律。方法:从中国期刊全文数据库中搜索慢性湿疹文献,提取中药处方,建立慢性湿疹中医用药数据库,进行统计分析。结果:治疗慢性湿疹以选用治血、水湿、风燥和止痒的药物为主。使用频率最高的的中药依次为当归（59.54%）、生地黄（50.38%）、白鲜皮（48.09%）、苦参（44.27%）、茯苓（41.98%）、地肤子（31.30%）、黄柏（29.77%）、丹参（29.00%）、荆芥（25.95%）、白芍（25.19%）、蝉蜕（24.43%）、刺蒺藜（21.37%）等。结论:慢性湿疹的中医用药以养血、凉血、活血、燥湿、利湿、祛风、健脾和止痒为主,应当分清复杂病理因素,选用适当的药物。关键词：慢性湿疹;用药规律;养血祛风
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发布时间：2024-09-23 - 摘要：<正>本刊讯8月17-19日,首届全国方剂组成原理高峰论坛在安徽举行。该论坛由中国实验方剂学杂志、天津中医药杂志、现代中药国家重点实验室联袂主办、北京东石时代国际文化传媒有限公司、安徽中医学院协办,中国中医科学院、天津中医药大学等单位给予大力支持,以岭医药集团、赛默
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发布时间：2024-09-23
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